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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Strongly peraluminous granites of Mesozoic in Eastern Nanling Range, southern China: Petrogenesis and implications for tectonics显示文摘The strongly peraluminous granites (SPGs) of Eastern Nanling Range (ENR) are a characteristic of all bearing highly aluminous minerals, such as muscovite±Al-rich biotite±tourmaline±garnet, and lack of cordierite. In respect of petrography, geochemistry, Nd isotope, and single grain zircon U-Pb dating, the representative granite bodies of them are studied. The research shows that these granites were emplaced in two stages, namely 228-225 Ma BP and J2-3 159-156 Ma BP, belonging to Indosinian and early Yanshanian periods, respectively, and they have low εNd(t) values (-10.6--11.1), high A/CNK, Rb/Sr ratios and tDM values (1887-1817 Ma), and REE's tetrad effect (TE1, 3=1.13-1.34). In comparison with related geology, petrology and chronology of granites in adjacent regions, it is suggested that Indosinian SPGs of ENR formed in the circumstance of post-collisional extension 20 Ma after the major collision of Indosinian Movement (258-243 Ma BP) in Indo-China Peninsula, and early Yanshanian SPGs formed in the background of back-arc extension setting controlled by paleo-Pacific tectonic domain, and J1, the interval of two stages, is the interim from Tethyan to Pacific tectonic domains in South China. These SPGs have similar geological and geochemical characteristics, because they all crystallized from the magma of partial melting of early Proterozoic metasedimentary rocks when the thickened crust (≤50 km) became thinning, decompression, and transmitting of water. | SUN Tao, ZHOU Xinmin, CHEN Peirong, LI Huimin, ZHOU Hongying, WANG Zhicheng & SHEN Weizhou Department of Earth Sciences, Nanjing University, Nanjing 210093, China Tianjin Institute of Geology and Mineral Resources, China Geological Survey, Tianjin 300170, China | 2005 | Science China Earth Sciences2005，48，2： | 78 |
| 3 | A competitive RT-PCR-based approach to quantifying promoter activity of stress gene显示文摘Chloramphenicol acetyltransferase (CAT) as a stable reporter has been broadly adopted to detect promoter activity of many stress genes in eukaryotic cells. A simplified, convenient and relatively accurate assay to reveal CAT activities driven by heat shock gene promoter upon cell stress is reported here. A CAT mutant control plasmid pMCAT is constructed out of pBLCAT3, with a DNA segment within the CAT gene deleted. The control plasmid pMCAT along with the wild type CAT reporter gene driven by the regulatory sequence of hsp90β gene are co-transfected into Jurkat cells. Following total cellular RNA extracted and reverse transcribed, PCR is carried out to amplify two individual fragments with the same pair of synthesized primers, which bind to the identical sequences in both pMCAT and the CAT reporter plasmids. The two PCR fragments amplified from the reporter and control RNAs are identified by their size differences in agarose gel electrophoresis. The ratio of intensity of the two PCR bands in the same lane is taken as 'relative units of promoter efficiency' of a gene. The transcripts instead of CAT activity thus detected, may not only provide a closer look at the promoter activity of the stress gene but also normalize the efficiency of transfection simultaneously. With experimental conditions optimized, visible and reliable results can be obtained without using any sophisticated instruments. Since the results obtained with the RT-PCR approach are reproducible, much less troublesome and time-consuming, and comparable to our previous data, we thus take it as a conventional procedure for promoter activity assay of human hsp gene upon heat shock. Additionally, results from the assay have recently provided the first evidence that Rac, MEKK and JNK signals participate in the heat shock-induced expression of hsp90β gene. | MO Zhicheng, LI Xiaoyan, LI Hongfan, CHENG Xiaokuan, WU Ninghua and SHEN Yufei(National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China) | 2002 | Progress in Natural Science:Materials International2002，12，10： | 4 |
| 4 | A compact polarization-integrated long wavelength infrared focal plane array based on In As/GaSb superlattice显示文摘Real-time polarization imaging plays a vital role in target detection,recognition,and tracking in complex scenes.However,large optical crosstalk and the integration technology are difficult problems in the development of polarization-integrated infrared detectors.We demonstrate a monolithic long wavelength infrared(LWIR)In As/Ga Sb superlattice focal plane array(FPA)integrated with the polarizer.To obtain high performance and real-time polarization imaging devices,the parameters of the grating were optimized and the optical crosstalk between adjacent polarization bands was studied.We analyze the light diffraction depending on the distance between the polarizer and photosensitive elements and the coupling between oblique incident light and device.The fabrication process of the antireflective polarizer,the integration technology of the polarizer and the FPA composed of 512×4×3 pixels were explored.Finally,a polarization integrated focal plane array(PI-FPA)was fabricated to detect polarized light with different oscillating directions.The large response extinction ratio of corresponding polarization bands was measured as 50:1,49:1,and 44:1 of 0°,60°,and 120°,respectively. | Jian ZHOU Yi ZHOU Ying SHI Fangfang WANG Zhicheng XU Zhizhong BAI Min HUANG Lulu ZHENG Zhaoming LIANG Yihong ZHU Qingqing XU Yiming SHEN Xiangxiao YING Jianxin CHEN | 2022 | Science China(Information Sciences)2022，65，2： | 2 |
| 5 | New insight into the action of supercritical carbon dioxide for grafting of maleic anhydride onto isotactic polypropylene by reactive extrusion 显示文摘 | Cao Kun Shen Zhicheng Yao Zhen | 2010 | Chemical Engineering Science2010，65，5： | 1 |
| 6 | Low-temperature formation of nanocrystalline β-SiC with high surface area and mesoporosity via reaction of mesoporous carbon and silicon powder显示文摘 | Zhicheng Liu Weihua Shen Wenbo Bu Hangrong Chen Zile Hua Lingxia Zhang Lei Li Jianlin Shi Shouhong Tan | 2005 | Microporous and Mesoporous Materials2005，，1： | 1 |
| 7 | Halogenated Thermally Activated Delayed Fluorescence Materials for Efficient Scintillation显示文摘Organic scintillators,materials with the ability to exhibit luminescence when exposed to X-rays,have aroused increasing interest in recent years.However,the enhancement of radioluminescence and improving X-ray absorption of organic scintillators lie in the inherent dilemma,due to the waste of triplet excitons and weak X-ray absorption during scintillation.Here,we employ halogenated thermally activated delayed fluorescence materials to improve the triplet exciton utilization and X-ray absorption simultaneously,generating efficient scintillation with a low detection limit,which is one order of magnitude lower than the dosage for X-ray medical diagnostics.Through experimental study and theoretical calculation,we reveal the positive role of X-ray absorption,quantum yields of prompt fluorescence,and intersystem crossing in promoting the radioluminescence intensity.This finding offers an opportunity to design diverse types of organic scintillators and expands the applications of thermally activated delayed fluorescence. | Xiao Wang Guowei Niu Zixing Zhou Zhicheng Song Ke Qin Xiaokang Yao Zhijian Yang Xiaoze Wang He Wang Zhuang Liu Chengzhu Yin Huili Ma Kang Shen Huifang Shi Jun Yin Qiushui Chen Zhongfu An Wei Huang | 2023 | Research2023，，4： | 1 |
| 8 | Low-temperature formation of nanocrystalline β-SiC with high surface area and mesoporosity via reaction of mesoporous carbon and silicon powder显示文摘 | Liu Zhicheng Shen Weihua Bu Wenbo | 2005 | Microporous and Mesoporous Materials2005，82，12： | 1 |
| 9 | New insight into the action of supercritical carbon dioxide for grafting of maleic anhydride onto isotactic polypropylene by reactive extrusion 显示文摘 | Cao Kun Shen Zhicheng Yao Zhen | 2010 | Chemical Engineering Science2010，65，5： | 1 |
| 10 | Spin-coating derived LSM-SDC films with uniform pore structure显示文摘 | Fei Ye Zhicheng Wang Wenjian Weng Kui Cheng Chenlu Song Piyi Du Ge Shen Gaorong Han | 2007 | Thin Solid Films2007，，16： | 1 |
| 11 | Preparation and performance of nanostructured porous thin cathode for low-temperature solid oxide fuel cells by spin-coating method显示文摘 | Zhicheng Wang Wenjian Weng Kui Chen Ge Shen Piyi Du Gaorong Han | 2007 | Journal of Power Sources2007，，1： | 1 |
| 12 | Enantiomeric characterization and structure elucidation of Otamixaban显示文摘Otamixaban is a potent(Ki=0.5 nM) fXa inhibitor currently in late-stage clinical development at Sanofi for the management of acute coronary syndrome. Being unproductive in obtaining a suitable crystal of Otamixaban, the required enantiomeric characterization has been accomplished using vibrational circular dichroism(VCD) spectroscopy. Selected by a spectrum similarity index, the calculated spectra of several higher energy conformers were found to match well with the observed spectra. The characteristic IR bands of these conformers were also identified and attributed to the solvation effect.Combined with both the single crystal x-ray diffraction results for an intermediate and the proton NMR study, the absolute configuration of Otamixaban is unambiguously determined to be(R,R). | Jian Shen Jiping Yang Winfried Heyse Harald Schweitzer Norbert Nagel Doris Andert Chengyue Zhu Vincent Morrison Gregory A.Nemeth Teng-Man Chen Zhicheng Zhao Timothy A.Ayers Yong-Mi Choi | 2014 | Journal of Pharmaceutical Analysis2014，4，3： | 1 |
| 13 | Low -temperature formation of nanocrystalline β - SiC with high surface area and mesoporosity via reaction of mesoporous carbon and silicon powder 显示文摘 | Liu Zhicheng Shen Weihua Bu Wenbo | 2005 | Micro- porous Mesoporons Mater2005，82，12： | 1 |
| 14 | Evolution of chitin-binding proteins in invertebrates显示文摘 | Marcelo J L | 1999 | J Mol Evol1999，48，： | 1 |
| 15 | The size and temperature effects of coercivity for the magnetic nanowire : Monte Carlo simulation 显示文摘 | Zhong Kehua Zhang Zhicheng Shen Baohua | 2007 | Solid State Phenomena Vols2007，，： | 1 |
| 16 | Evolution of chitin-binding proteins in invertebrates显示文摘 | Marcelo J L | 1999 | J Mol Evol1999，48，： | 1 |
| 17 | Achieving data-driven actionability by combining learning and planning显示文摘 | Qiang LV Yixin CHEN Zhaorong LI Zhicheng CUI Ling CHEN Xing ZHANG Haihua SHEN | 2018 | Frontiers of Computer Science2018，12，5： | 1 |
| 18 | Preparation and properties of the composite core shell latex of poly ( 3 - methacryloxylpropyltrimethoxylsilane)/poly(methyl acrylate) induced by gamma ray radiation显示文摘 | Zou Mingxuan Zhicheng Zhang Shen Xuefeng | 2005 | Radiation Physics and Chemistry2005，74，： | 1 |
| 19 | Therapeutic effect of ketogenic diet treatment on type 2 diabetes显示文摘Diet plays an important role in diabetes development.The effect of ketogenic diet on type 2 diabetes remains elusive.In this study,we collect diabetes related physiological parameters and abdominal MRI images to evaluate the effect of ketogenic diet after 3-months of consecutive treatment.Ketogenic diet mitigates the diabetes symptom inferring from the statistically significant reduction of key diabetic physiological parameters such as Hemoglobin A1c(HbA1c)concentration,Triglyceride(TG),Fasting blood glucose(FBG),Body Mass Index(BMI),and adipose tissue volume.Moreover,we propose a new parameter to quantify the treating effect of ketogenic diet since Slope and Offset of Oral Glucose Tolerance Test(SOOGTT)incorporate more sampling points by fitting OGTT(Oral Glucose Tolerance Test)curve.SOOGTT shows statistically significant(P<0.001)treating effect of ketogenic diet on type 2 diabetes.We use artificial intelligence to segment adipose tissue for treatment evaluation,which shows that ketogenic diet reduces the amount of adipose tissue. | Fang Li Yang Shen Qun Chen Xingyu Li Hongnan Yang Canyang Zhang Jinjun Lin Zhicheng Du Chenyao Jiang Chengming Yang Dongmei Yu Peiwu Qin | 2022 | Journal of Future Foods2022，2，2： | 0 |
| 20 | An Implicit Solver for the Time-Dependent Kohn-Sham Equation显示文摘The implicit numerical methods have the advantages on preserving the physical properties of the quantum system when solving the time-dependent Kohn-Sham equation.However,the efficiency issue prevents the practical applications of those implicit methods.In this paper,an implicit solver based on a class of Runge-Kutta methods and the finite element method is proposed for the time-dependent Kohn-Sham equation.The efficiency issue is partially resolved by three approaches,i.e.,an h-adaptive mesh method is proposed to effectively restrain the size of the discretized problem,a complex-valued algebraic multigrid solver is developed for efficiently solving the derived linear system from the implicit discretization,as well as the OpenMP based parallelization of the algorithm.The numerical convergence,the ability on preserving the physical properties,and the efficiency of the proposed numerical method are demonstrated by a number of numerical experiments. | Lei Yang Yedan Shen Zhicheng Hu Guanghui Hu | 2021 | Numerical Mathematics(Theory,Methods and Applications)2021，14，1： | 0 |
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