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| 1 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 2 | Magnetic resonance susceptibility weighted imaging in detecting intracranial calcification and hemorrhage显示文摘背景计算断层摄影术(CT ) 在检测 intracranial 石灰化比平淡的磁性的回声成像(MRI ) 好。试图估计在 intracranial 石灰化和 hemorrhage.Methods 的察觉和区别的加权的成像(SWI ) 在这研究注册了的先生危险性的价值的这研究是包括 CT 表明的石灰化的 13 个盒子和 intracerebral 出血的 22 个盒子的 35 个病人。在所有这些题目使用的先生序列包括了轴的 T1WI, T2WI 和 SWI。SWI 上的石灰化和出血的阶段移动(PS ) 被计算,他们的信号在改正的阶段图象上展示被比较。在检测 intracranial 石灰化和出血的 T1WI, T2WI 和 SWI 的敏感是为头部的石灰化的 SWI 的察觉率是的分析 statistically.Results 98.2% ,比 T1 Wl 和 T2WI 的显著地高。它不与 CT 的显著地不同(P > 0.05 ) 。在不同阶段有 49 出血性的损害检测 n SWI, 30 在 T2WI 上并且 18 在 T1WI 上。石灰化和出血的平均 PS 是在检测的 +0.734han 常规 MRI 微出血, SWI 可以在区分与石灰化或出血联系的服的疾病起一个重要作用。 | ZHU Wen-zhen QI Jian-pin ZHAN Chuan-jia SHU Hong-ge ZHANG Lin WANG Cheng-yuan XIA Li-ming HU Jun-wu FENG Ding-yi | 2008 | Chinese Medical Journal2008，，20： | 26 |
| 3 | Emodin and baicalein inhibit pancreatic stromal derived factor-1 expression in rats with acute pancreatitis显示文摘BACKGROUND: Stromal derived factor-1 (SDF-1) is an efficacious leukocyte chemoattractant, which can attract lymphocytes and mononuclear cells from bloodstream into the site of inflammation. Emodin, an anthraquinone derivative from Radix et Rhizoma Rhei, and baicalein, a flavone from Scutellaria baicalensis Georgi, both have been reported to possess anti-inflammatory activities. The expression pattern of SDF-1 in experimental acute pancreatitis (AP) and the effect of emodin or baicalein on that are not well defined. The present study aimed to investigate the effects of emodin and baicalein on pancreatic myeloperoxidase (MPO) activity (reflecting leukocyte sequestration) and cytokine production, as well as tissue SDF-1 expression in the setting of AP. METHODS: A rat model of AP was induced by administration of 5% sodium taurocholate through the biliopancreatic duct. The level of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and MPO in the pancreas, and serum amylase were tested by immunohistochemistry, ELISA and chromatometry. The expressions of SDF-1α and SDF-1β were detected by real-time PCR, Western blotting, and immunohistochemistry. RESULT: Combination of emodin and baicalein signifi- cantly reduced pancreatic TNF-α, IL-6 and MPO, and also inhibited pancreatic SDF-1 expression.CONCLUSIONS: The inhibition of SDF-1 expression by emodin and baicalein might contribute, in part at least, to the amelioration of pancreatic inflammation. The present study also shows benefits of simultaneous treatment of AP. | Li, Zong-Fang Xia, Xian-Ming Huang, Chen Zhang, Shu Zhang, Jian Zhang, Ai-Jun | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，2： | 20 |
| 4 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 5 | Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice显示文摘Objective To determine the ability of grape seed proanthocyanidin extract(GSPE) in alleviating arsenic-induced reproductive toxicity. Methods Sixty male Kunming mice received the following treatments by gavage: normal saline solution(control); arsenic trioxide(ATO; 4 mg/kg); GSPE(400 mg/kg); ATO+GSPE(100 mg/kg); ATO+GSPE(200 mg/kg) and ATO+GSPE(400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor(erythroid-derived 2)-like 2(Nrf2), heme oxygenase 1(HO1), glutathione S-transferase(GST), NAD(P)H dehydrogenase, and quinone 1(NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase(SOD), glutathione(GSH), total antioxidative capability(T-AOC), malondialdehyde(MDA), 8-hydroxydeoxyguanosine(8-OHd G), and reproductive indexes were analyzed. Results ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO +GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of m RNA expression of Nrf2, HO, NQO1, and GST. Conclusion GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity. | LI Shu Gang DING Yu Song NIU Qiang XU Shang Zhi PANG Li Juan MA Ru Lin JING Ming Xia FENG Gang Ling LIU Jia Ming GUO Shu Xia | 2015 | Biomedical and Environmental Sciences2015，28，4： | 14 |
| 6 | Exosomes derived from human amniotic epithelial cells accelerate diabetic wound healing via PI3K-AKT-mTOR-mediated promotion in angiogenesis and fibroblast function显示文摘Background:Diabetic wounds are one of the most common and serious complications of diabetes mellitus,characterized by the dysfunction of wound-healing-related cells in quantity and quality.Our previous studies revealed that human amniotic epithelial cells(hAECs)could promote diabetic wound healing by paracrine action.Interestingly,numerous studies demonstrated that exosomes derived from stem cells are the critical paracrine vehicles for stem cell therapy.However,whether exosomes derived from hAECs(hAECs-Exos)mediate the effects of hAECs on diabetic wound healing remains unclear.This study aimed to investigate the biological effects of hAECs-Exos on diabetic wound healing and preliminarily elucidate the underlying mechanism.Methods:hAECs-Exos were isolated by ultracentrifugation and identified by transmission electron microscopy,dynamic light scattering and flow cytometry.A series of in vitro functional analyses were performed to assess the regulatory effects of hAECs-Exos on human fibroblasts(HFBs)and human umbilical vein endothelial cells(HUVECs)in a high-glycemic microenvironment.Highthroughput sequencing and bioinformatics analyses were conducted to speculate the related mechanisms of actions of hAECs-Exos on HFBs and HUVECs.Subsequently,the role of the candidate signaling pathway of hAECs-Exos in regulating the function of HUVECs and HFBs,as well as in diabetic wound healing,was assessed.Results:hAECs-Exos presented a cup-or sphere-shaped morphology with a mean diameter of 105.89±10.36 nm,were positive for CD63 and TSG101 and could be internalized by HFBs and HUVECs.After that,hAECs-Exos not only significantly promoted the proliferation and migration of HFBs,but also facilitated the angiogenic activity of HUVECs in vitro.High-throughput sequencing revealed enriched miRNAs of hAECs-Exos involved in wound healing.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses have shown that the target genes of the top 15 miRNAs were highly enriched in the PI3K-AKT pathway.Further functional studies demonstrated that the PI3K-AKT-mTOR pathway was necessary for the induced biological effects of hAECs-Exos on HFBs and HUVECs,as well as on wound healing,in diabetic mice.Conclusions:Our findings demonstrated that hAECs-Exos represent a promising,novel strategy for diabetic wound healing by promoting angiogenesis and fibroblast function via activation of the PI3K-AKT-mTOR pathway. | Pei Wei Chenjian Zhong Xiaolan Yang Futing Shu Shichu Xiao Teng Gong Pengfei Luo Li Li Zhaohong Chen Yongjun Zheng Zhaofan Xia | 2020 | Burns & Trauma2020，8，1： | 12 |
| 7 | Diet and gastric cancer:a case-control study in Fujian Province,China显示文摘NTRODUCTIONChangleandFuqingcitiesarelocatedinthesoutheasternpartofFujianProvince,Chinawithahighincidenceofgastriccancer.Howev... | YE Wei Min 1, YI Ying Nan 1, LUO Ren Xia 2, ZHOU Tian Shu 3, LIN Ru Tao 1 and CHEN Gui Dong 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 12 |
| 8 | Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens显示文摘Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1~3CFU) M.pneumoniae DNA.The sensitivity of MpP1,RepMp1,and Mp181 assays appeared to be 100%,100%,and 85%,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens. | ZHAO Fei CAO Bin HE Li Hua YIN Yu Dong TAO Xiao Xia SONG Shu Fan MENG Fan Liang ZHANG Jian Zhong | 2012 | Biomedical and Environmental Sciences2012，25，1： | 11 |
| 9 | Heterogeneous oxidation of cyclohexanone catalyzed by TS-1:Combined experimental and DFT studies显示文摘 | Changjiu Xia Long Ju Yi Zhao Hongyi Xu Bin Zhu Feifei Gao Min Lin Zhenyu Dai Xiaodong Zou Xingtian Shu | 2015 | 催化学报2015，36，6： | 11 |
| 10 | Analysis of detection capabilities of LEO reconnaissance satellite constellation based on coverage performance显示文摘In the design problem of low earth orbit(LEO) reconnaissance satellite constellation, optimization of coverage performance is the design goal in most current methods. However,in the using process, the user only concerns with the detection capabilities rather than coverage performance. To establish the relationship between these two aspects, the reconnaissance processes of normal stochastic targets are considered and the mathematic models of detection processes are built. The indicators of coverage performance are used to evaluate the detection probability and expectation of detection time delay, which are important factors in reconnaissance constellation estimation viewed from military intelligence discipline. The conclusions confirmed by the final simulation will be useful in LEO reconnaissance constellation design, optimization and evaluation. | MENG Shaofei SHU Jiansheng YANG Qi XIA Wei | 2018 | Journal of Systems Engineering and Electronics2018，29，1： | 10 |
| 11 | Apoptosis of Spodoptera litura larval hemocytes induced byheavy metal zinc显示文摘By adding different amount of zinc into the artificial medium of the insect larvae, the zinc-induced apoptosis of the larvae haemocytes of the herbivorous insect Spodoptera litura Fabricius was investigated with flow cy- tometer. The results showed that the increase of zinc dose in the artificial feed led to the accumulations of zinc in the lar- val hemolymph and fat body, and more zinc was accumu- lated in fat body than in hemolymph. The apoptosis of hemocytes was significantly induced at high zinc concentra- tion (1000 mg·kg?1) in the insect diet, and the apoptosis rate was 63.63%, which was remarkably higher than that at con- trol and lower concentrations (50―500 mg·kg?1). This sug- gests that the high dose of zinc in the artificial diet of S. litura larvae could induce the apoptosis of the larval hemocytes of S. litura. | XIA Qiang SUN Hongxia HU Xinjun SHU Yinghua GU Dexiang ZHANG Guren | 2005 | Chinese Science Bulletin2005，50，24： | 9 |
| 12 | mTORC1 inhibitor RAD001(everolimus)enhances non-small cell lung cancer cell radiosensitivity in vitro via suppressing epithelial-mesenchymal transition显示文摘Resistance to radiotherapy causes non-small cell lung cancer (NSCLC) treatment failure associated with local recurrence and metastasis. Thus, understanding the radiosensitization of NSCLC cells is crucial for developing new treatments and improving prognostics. mTORC1 has been shown to regulate tumor cell radiosensitivity, but the underlying mechanisms are unclear. Moreover, mTORC1 also regulates epithelial–mesenchymal transition (EMT) that is important to metastasis and recurrence. In this study we explored whether mTORC1 regulated NSCLC cell radiosensitivity by altering EMT. We performed immunohistichemical analysis using tumor, adjacent and normal tissues from 50 NSCLC patients, which con?rmed signi?cantly elevated mTOR protein expression in NSCLC tissue. Then we used NCI-H460 and NCI-H661 cell lines to examine the effects of the mTORC1 inhibitor RAD001 (everolimus) on in vitro radiosensitivity, protein expression and dose-survival curves. RAD001 (10 nmol/L) signi?cantly inhibited the mTORC1 pathway in both the cell lines. Pretreatment with RAD001 (0.1 nmol/L) enhanced the radiosensitivity in NCI-H661 cells with wild-type PIK3CA and KRAS but not in NCI-H460 cells with mutant PIK3CA and KRAS;the sensitivity enhancement ratios in the two NSCLC cell lines were 1.40 and 1.03, respectively. Furthermore, pretreatment with RAD001 (0.1 nmol/L) signi?cantly decreased the migration and invasion with altered expression of several EMT-associated proteins (signi?cantly increased E-cadherin and decreased vimentin expression) in irradiated NCI-H661 cells. Publicly available expression data con?rmed that irradiation affected mTOR and EMT-associated genes at the transcript level in NSCLC cells. These results suggest that mTORC1 inhibition enhances the in vitro radiosensitivity of NSCLC cells with wild-type PIK3CA and KRAS by affecting EMT. Our preclinical data may provide a potential new strategy for NSCLC treatment. | Yu Chen Wen-wen LI Ping Peng Wei-heng Zhao Yi-jun Tian Yu Huang Shu Xia Yuan Chen | 2019 | Acta Pharmacologica Sinica2019，40，8： | 8 |
| 13 | Predictive validity of BODE index for anxious and depressive symptoms in patients with chronic obstructive pulmonary disease显示文摘背景焦虑和消沉是长期的妨碍的肺的疾病(COPD ) 的二最普通、很可修改的 comorbidities 并且在健康和预后上有独立效果。FEV1% 被显示了是焦虑和消沉的一个差的预言者。身体团索引,气流阻塞的度,呼吸困难,和锻练能力(预示) 索引是可以在 COPD 病人预言健康结果的一个多维的评价系统。这研究的目的是在这是的 COPD patients.Methods 为焦急、压抑的症状调查 BODE 索引的预兆的有效性 multicenter 在有稳定的 COPD 的 256 个病人的未来的代表性的学习。焦急、压抑的症状用医院焦虑和消沉规模(HADS ) 被估计。在焦虑,消沉和潜在的预言者之间的关系(包括 BODE 索引) 被一个二进制逻辑回归模型分析。焦急、压抑的结果题目走了更短的六分钟的走距离(6MWD ) ,有更多的呼吸困难,一更高预示索引,并且降低生活的健康相关的质量(P < 0.01 ) 。焦虑和萧条分数显著地被相关与预示指数,分别地(r=0.335, P < 0.001;r=0.306, P < 0.001 ) 。焦虑和消沉的流行增加了与预示舞台增加(P < 0.05 ) 。根据二进制逻辑回归,因为它包括了呼吸困难和 6MWD, BODE 索引是焦虑和消沉的一个好、独立的预言者,它被显示是主要 determinants.Conclusions 为焦虑和消沉的 BODE 索引的预兆的有效性被表明。我们建议 BODE 索引应该在对 COPD 严厉的评价被包括。 | AN Li LIN Ying-xiang YANG Ting ZHANG Hong JIAO Xia ZHANG Shu CHANG Xiao-hong WANG Zhao-mei WANG Chen | 2010 | Chinese Medical Journal2010，，14： | 8 |
| 14 | Genome analysis and signature discovery for diving and sensory properties of the endangered Chinese alligator显示文摘Crocodilians 正在潜水能为时间的长时期在水下面屏息并且是有优秀感觉能力的黄昏的动物的爬行动物。他们包括鸟的一个姐妹系并且没有性染色体。这里,我们报导危及的中国短吻鳄(短吻鳄 sinensis ) 的染色体顺序并且描述它的唯一的特征。下一代的定序产生了未加工的顺序的 314 Gb,产出 2.3 Gb 的一种染色体尺寸。22 200 基因的一个总数用 de novo 在短吻鳄 sinensis 被预言,基于相同、基于 RNA 的联合模型。长潜水的行为的基因基础包括酸式碳酸盐绑定血红素基因,磷酸盐绑定和特殊酸式碳酸盐绑定氧搬运的常规的 co-functioning,和断然选择的精力的复制新陈代谢,铵酸式碳酸盐排泄和心肌收缩。进一步,我们阐明了柔韧的短吻鳄 sinensis 感觉系统包括一个显著地扩展的嗅觉的受体全部剧目,很快演变神经相关的细胞的部件和视觉感觉,和夜里的积极选择视觉相关的视蛋白和健全联系察觉的 otopetrin。我们也为抗菌剂肽的天生的免疫和表示为分成三部分的包含主题的 C 类型 lectin 和 butyrophilin 基因的适应免疫以及扩大与系特定的抗原演讲基因的一个可观的数字发现了一个开发得好的免疫系统。在 situ 杂交的 Multifluorescence 显示出那个短吻鳄染色体 3,它编码 DMRT1,与鸡染色体 Z 一起展出重要 synteny。最后,人口历史分析百万年以前显示了人口混合 0.60-1.05,当 Qinghai 西藏的高原被高举时。 | Qiu-Hong Wan Sheng-Kai Pan Li Hu Ying Zhu Peng-Wei Xu Jin-Quan Xia Hui Chen Gen-Yun He Jing He Xiao-Wei Ni Hao-Long Hou Sheng-Guang Liao Hai-Qiong Yang Ying Chen Shu-Kun Gao Yun-Fa Ge Chang-Chang Cao Peng-Fei Li Li-Ming Fang Li Liao Shu Zhang Meng-Zhen Wang Wei Dong Sheng-Guo Fang | 2013 | Cell Research2013，23，9： | 8 |
| 15 | Comparison of activated carbon and iron/cerium modified activated carbon to remove methylene blue from wastewater显示文摘The methylene blue(MB)removal abilities of raw activated carbon and iron/cerium modified raw activated carbon(Fe–Ce-AC)by adsorption were researched and compared.The characteristics of Fe–Ce-AC were examined by N_2adsorption,zeta potential measurement,FTIR,Raman,XRD,XPS,SEM and EDS.After modification,the following phenomena occurred:The BET surface area,average pore diameter and total pore volume decreased;the degree of graphitization also decreased.Moreover,the presence of Fe_3O_4led to Fe–Ce-AC having magnetic properties,which makes it easy to separate from dye wastewater in an external magnetic field and subsequently recycle.In addition,the equilibrium isotherms and kinetics of MB adsorption on raw activated carbon and Fe–Ce-AC were systematically examined.The equilibrium adsorption data indicated that the adsorption behavior followed the Langmuir isotherm,and the pseudo-second-order model matched the kinetic data well.Compared with raw activated carbon,the maximum monolayer adsorption capacity of Fe–Ce-AC increased by27.31%.According to the experimental results,Fe–Ce-AC can be used as an effective adsorbent for the removal of MB from dye wastewater. | Song Cheng Libo Zhang Aiyuan Ma Hongying Xia Jinhui Peng Chunyang Li Jianhua Shu | 2018 | Journal of Environmental Sciences2018，30，3： | 8 |
| 16 | Commuting Dual Toeplitz Operators on the Polydisk显示文摘在 polydisk 上,双 Toeplitz 操作员的交换性被学习。变换双 Toeplitz 操作员,实质上变换的双 Toeplitzoperators 和实质上变换半的双 Toeplitz 操作员的 Weobtain 描述。 | Yu Feng LU Shu Xia SHANG | 2007 | Acta Mathematica Sinica,English Series2007，23，5： | 8 |
| 17 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 18 | Successful Production of an All-Female Common Carp (Cyprinus carpio L.) Population Using cyp17a1-Deficient Neomale Carp显示文摘Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus. | Gang Zhai Tingting Shu Kuangxin Chen Qiyong Lou Jingyi Jia Jianfei Huang Chuang Shi Xia Jin Jiangyan He Donghuo Jiang Xueqiao Qian Wei Hu Zhan Yin | 2022 | Engineering2022，8，1： | 7 |
| 19 | Functional analysis of low-density lipoprotein receptor in homozygous familial hypercholesterolemia patients with novel 1439 C →T mutation of low-density lipoprotein receptor gene显示文摘背景家庭血胆脂醇过多(FH ) ,由低密度引起了脂蛋白(LDL ) 受体(LDL-R ) 基因变化,与早熟的冠的心疾病的增加的风险被联系。直到现在,有关 FH 的有限分子的数据在中国是可得到的。现在的学习描述了临床的侧面和房间有新奇 LDL-R 基因 mutation.Methods 的一个中国 FH 家族的生物缺点病人编码区域的 LDL-R 基因被定序。病人的淋巴细胞被孤立, LDL-R 表达式,绑定和举起函数被 immunohistochemistry 染色和流动 cytometry 察觉观察。病人的心和主要大容器被容器超声检查和病人的 LDL-R 表示, LDL 绑定和举起功能是的心肌的灌注成像(MPI ).Results 检测比正常控制显著地低(39% , 63% 和 76% 分别地) 。一篇小说同型结合 LDL-R 基因的 1439 CT 变化在病人和他的家庭被检测。ECG 显示出不正常的心绞痛。Echocardiogram 显示出冠的动脉的狭窄和大动脉的阀门和它的根的石灰化。血容器超声检查显示出大容器 intima 的厚度,并且容器腔被 71% 缩小。MPI 显示出的 ischemic changes.Conclusions FH 病人的 LDL-R 合成机能障碍导致动脉的狭窄和石灰化,它是临床的混乱的主要显型。LDL-R 基因的变化是坚定的。这些数据增加在中国的 FH 的 mutational 光谱。 | LIN Jie WANG Lu-ya LIU Shu XIA Jun-hui YONG Qiang DU Lan-ping PAN Xiao-dong XUE Hong CHEN Bao-sheng JIANG Zhi-sheng | 2008 | Chinese Medical Journal2008，，9： | 7 |
| 20 | Androgen signaling regulates the transcription of anti-Müllerian hormone via synergy with SRY-related protein SOX9A显示文摘Anti-Müllerian hormone(amh) is one of the earliest functional genes expressed during testicular differentiation. It has been suggested that androgen signaling regulates critical genes for the differentiation and development of the testis. To elucidate the exact regulatory mechanisms involved in amh transcription mediated by androgen signaling, androgen signaling was manipulated in zebrafish by cytochrome P45017a1(cyp17a1) knockout and Flutamide treatment. In cyp17a1-deficient and Flutamide-treated testes,up-regulated sry-box9a(sox9a) and down-regulated amh were observed. Moreover, a physical association of the zebrafish androgen receptor(AR) and SOX9 A was found. The interaction between AR and SOX9 A was mediated via the DNA binding domain(DBD) of AR and the transactivation domain(TA) of SOX9 A,and was further enhanced by 5-alpha dihydrotestosterone(DHT), one of the most potent androgens.Intriguingly, together with SOX9 A, androgen signaling synergistically promoted amh transcription,mainly through the proximal 1 kb of the amh promoter region. Taken together, our data demonstrate a critical mechanism underlying the direct synergy of androgen signaling and SOX9 A in the regulation of amh transcription. | Gang Zhai Tingting Shu Yuguo Xia Xia Jin Jiangyan He Zhan Yin | 2017 | Science Bulletin2017，62，3： | 7 |
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