|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Aptamer-quantum dots and teicoplanin-gold nanoparticles constructed FRET sensor for sensitive detection of Staphylococcus aureus显示文摘The detection of bacterial pathogen such as Staphylococcus aureus(S.aureus) is essential for the regulation of food hygiene and disease diagnosis.Herein,we developed a simple one-step fluorescence resonance energy transfer(FRET)-based sensor for specific and sensitive detection of S.aureus in food and serum samples,in which aptamer-modified quantum dots(aptamer-QDs) was employed as the energy donor and antibiotic of teicoplanin functionalized-gold nanoparticles(Teico-AuNPs) was chosen as the energy acceptor.Within 1 h,the FRET-based sensor showed a linear range of from 10 cfu/mL to 5 × 10^(8) cfu/mL,with the low limit of detection(LOD,2 cfu/mL) for S.aureus in buffer.When further applied to assay S.aureus in real samples,the FRET-based sensor showed good recoveries ranging from 84.5% to 110.0%,with relative standard derivations(RSDs) of 0.01%-0.44% and a LOD of 100 cfu/mL in milk,orange juice and human serum. | Xiaoqi Tao Ziyi Liao Yaqing Zhang Fei Fu Mengqi Hao Yang Song Erqun Song | 2021 | Chinese Chemical Letters2021，32，2： | 5 |
| 2 | Higher dietary protein increases growth performance,anti-oxidative enzymes activity and transcription of heat shock protein 70 in the juvenile sea urchin(Strongylocentrotus intermedius)under a heat stress显示文摘This study was conducted to investigate the effect of dietary protein concentration(12%,18%,24%,30% and 36%)on the growth performance,activity of anti-oxidative enzymes and heat shock protein 70(HSP70)transcription in the sea urchin(Strongylocentrotus intermedius)under a heat stress.After 112 days of feeding trial the sea urchins were heat stressed(26C)and the coelomic fluid and intestine sampled at time 0 and 15 min,2 h and 6 h.The results showed that an increase in dietary protein(12%-24%),significantly increased(p<0.05)the sea urchin weight gain rate(WGR).As dietary protein increased(from 18% to 36%),the gonadosomatic index(GI)of juvenile sea urchins also significantly increased(p<0.05)from 18.0%to 22.6%.Superoxide dismutase(SOD)activity increased with dietary protein increase(12%-30%)and the enzyme activity was significantly higher(p<0.05)in the coelomic fluid of sea urchins that were fed with 30% diets when compared to 12% and 36% protein diets at all time points after the heat stress.Catalase(CAT)activity showed a similar tendency with the increase in dietary protein concentration at time 0 and 15 min after the heat stress(p<0.05).Transcription of HSP70 in the intestine also showed a similar trend to SOD and was highest in the animals that were fed with 30% protein diets(p<0.05).Our results suggest that 24% protein diets could meet the requirements for growth performance but a 30% protein diet resulted in improved gonad development and anti-heat stress capacity in this sea urchin species. | Rantao Zuo Shouquan Hou Fanxiu Wu Jian Song Weijie Zhang Chong Zhao Yaqing Chang | 2017 | Aquaculture and Fisheries2017，2，1： | 2 |
| 3 | Sequential immunizations confer cross-protection against variants of SARS-CoV-2,including Omicron in Rhesus macaques显示文摘Variants of concern(VOCs)like Delta and Omicron,harbor a high number of mutations,which aid these viruses in escaping a majority of known SARS-CoV-2 neutralizing antibodies(NAbs).In this study,Rhesus macaques immunized with 2-dose inactivated vaccines(Coronavac)were boosted with an additional dose of homologous vaccine or an RBD-subunit vaccine,or a bivalent inactivated vaccine(Beta and Delta)to determine the effectiveness of sequential immunization.The booster vaccination significantly enhanced the duration and levels of neutralizing antibody titers against wild-type. | Wei Deng Qi Lv Fengdi Li Jiangning Liu Zhiqi Song Feifei Qi Qiang Wei Pin Yu Mingya Liu Shasha Zhou Yaqing Zhang Hong Gao Nan Wang Zijing Jia Kai Gao Jiayi Liu Chong Xiao Haiquan Shang Xiangxi Wang Linlin Bao Chuan Qin | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 2 |
| 4 | Relationships between body weight and other morphological traits in young sea cucumbers Apostichopus japonicas显示文摘Correlation, multiple regression, and path analyses were used to investigate the relationships between body weight and three other morphological traits in juvenile Japanese sea cucumbers Apostichopus japonicus . We measured live body weight (BW), body length (BL), numbers of papillae (NP), and numbers of tube feet (NF) at 60, 80, 100, and 130 days post-hatching (dph). We calculated path correlation coefficients, correlation indices ( R 2 ), and coefficients of determination with BW as the dependent variable and the other morphological traits as independent variables. The coefficient of variation for BW was high across all age groups, and all measured morphological traits were significantly correlated ( P < 0.01). BL had the greatest direct eff ect on BW across all age groups (60 dph, 0.526;80 dph, 0.404;100 dph, 0.620;and 130 dph, 0.681), while NF had the greatest indirect eff ect on BW across all age groups (60 dph, 0.528;80 dph, 0.452;100 dph, 0.666;and 130 dph, 0.603). Regression analyses between morphological traits and BW indicated that R 2 was greater than 0.85 only in the 100-dph specimens. The indirect eff ects of the other measured morphological traits on BW were age-dependent. The optimal regression equations, as determined with stepwise regression, were, for 60-dph specimens: BW 60 =10 (-3.04+0.092BL+0.014NP+0.014NF)( R 2 =0.632);for 80-dph specimens: BW 80 =10 (- 3.035+0.056BL+0.017NP+0.02NF)( R 2 =0.686);for 100-dph specimens: BW 100 =10 (- 3.742+0.069BL+0. 633 · lg (NP)+0. 464 · lg (NF))( R 2 =0.893);and for 130-dph specimens: BW 130 =10 (- 2.472+0.065BL+0.012NP)( R 2 =0.774). Our work clarified the correlation between various morphological traits and body weight of a commercially-important sea cucumber species ( A . japonicus ). Our predictive models for body weight might be useful for the aquaculture and selective breeding of A . japonicus . These models might also provide theoretical support for the indirect selection of traits that are difficult to select directly. | ZHAN Yaoyao ZHANG Weijie GE Chen LIN Kai LI Guang SONG Jian CHANG Yaqing | 2019 | Journal of Oceanology and Limnology2019，37，2： | 2 |
| 5 | Corrigendum to“SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip”[Sci.Bull.(2021)66(8)783-793]显示文摘The authors would like to correct Fig.2g.An annotation error was introduced in the preparation of this figure for publication.The authors declare that this correction does not change the results or conclusions of this paper.The authors sincerely apologize for having this error in the article,and apologize for any inconvenience caused. | Yaqiong Guo Ronghua Luo Yaqing Wang Pengwei Deng Tianzhang Song Min Zhang Peng Wang Xu Zhang Kangli Cui Tingting Tao Zhongyu Li Wenwen Chen Yongtang Zheng Jianhua Qin | 2021 | Science Bulletin2021，66，20： | 2 |
| 6 | Tall gastrodis tuber combined with antiepileptic drugs repairs abnormal perfusion foci in focal epilepsy显示文摘One hundred patients with focal epilepsy were recruited for the present study and their seizures controlled with antiepileptic drugs. The patients then orally received a capsule of tall gastrodis tuber powder, a traditional Chinese drug, and underwent single photon emission computed tomography, long-term electroencephalogram, and CT/MRI. Blood drug levels were monitored throughout the study. Before treatment with tall gastrodis tuber, 35 of the 100 cases had abnormal CT/MRI scans; 79 cases had abnormal single photon emission computed tomography images; 86 cases had abnormal electroencephalogram; and a total of 146 abnormal perfusion foci were observed across the 100 subjects. After treatment, the number of patients with normal single photon emission computed tomography images increased by 12; normal electroencephalogram was observed in an additional 27 cases and the number of patients with epileptiform discharge decreased by 29 (34% of 86); the total number of abnormal perfusion foci decreased by 52 (36%) and changes in abnormal foci were visible in 65 patients. These changes indicate that the administration of tall gastrodis tuber in combination with antiepileptic drugs repairs abnormal perfusion foci in patients with focal epilepsy. Our results demonstrate that traditional Chinese drugs can repair abnormal perfusion foci and, as such, are a promising new pathway in the treatment of focal epilepsy. | Weimin Wang Zhenyu Fan Yongqin Zhang Yuxia Yang Yaqing Liu Xiaoli Dang Wenjun Song Yinping Wu Jiang Ye | 2013 | Neural Regeneration Research2013，8，3： | 2 |
| 7 | A preliminary genetic map of Zhikong scallop(Chlamys farreri Jones et Preston 1904)显示文摘 | Linsheng Song Yaqing Chang | 2005 | Aquaculture Reasearch2005，36，7： | 1 |
| 8 | Dissipation of polycyclic aromatic hydrocarbons in crop soils amended with oily sludge显示文摘Oil fields present a potential ecological risk to nearby farmland soil. Here we present a new method designed to evaluate the ability of winter wheat(Triticum aestivum) to contribute to the dissipation of polycyclic aromatic hydrocarbons(PAHs), which are priority pollutants in soils contaminated by oily sludge. The influence of different doses of oily sludge on the dissipation of PAHs was studied along with individual PAH profiles in soils after different periods of plant growth. Five soil samples were artificially contaminated with different percentages of oily sludge(0 %, 5 %, 10 %, 15 % and 20 %). Winter wheat grew in the oily sludge–amended soils for 265 days.PAH content in the soils was monitored over the course of the study. The rate of PAH dissipation is related to the properties of different PAHs, period of winter wheat growth, and oily sludge application dose. Analysis for treated soils indicates that the dissipation of PAHs increased significantly over the first 212 days, followed by minimal changes over the final 53 days of treatment. In contrast, PAH dissipation slowed with increasing oily sludge application. For each PAH, the experimental results showed a significant compound-dependent trend. Winter wheat in the present study significantly enhanced the dissipation of PAHs in oily sludge–contaminated soil. | Shaoping Kuang Wenjuan Yu Yan Song Yaqing Su Huihui Wang Lina Wang | 2016 | Acta Geochimica2016，35，4： | 1 |
| 9 | Isolation of a New PAK1 Gene from Sea Cucumber(Apostichopus japonicus) and Its Expression Analysis and Function Characterization显示文摘The p21-activated kinase 1(PAK1)is a downstream serine/threonine kinase effector of Rac1/Cdc42 that regulates various biological processes including those associating with pathological inflammation.To investigate the function of PAK1 in echinoderms,we isolated a new PAK1 from sea cucumber Apostichopus japonicus(AjPAK1)by transcriptome database mining and with rapid amplification of cDNA ends(RACE).The full-length cDNA AjPAK1 was 2303 bp in length,containing a 1587 bp ORF encoding 528 amino acid residues.The deduced AjPAK1 contained a p21-Rho-binding domain(PBD)and a serine/threonine protein kinase catalytic domain(S_TKc),which was similar to the PAK1 of crown-of-thorns starfish Acanthaster planci and other eukaryotes.AjPAK1 expressed in all tissues of adult A.japonicus analyzed with the highest transcript anumdance detected in coelomocytes.Significant change in AjPAK1 abundance was observed at 4,24,48 and 72 h after Vibrio splendidus infection.Silencing AjPAK1 induced a significant reduction of lysozyme content in coelomic fluid and relative transcript abundances of AjRac1 and AjMKK3/6 in A.japonicus coelomocytes.These results should aid to characterize PAK1 of sea cucumber and decipher its immune function. | REN Liyuan LI Kaiquan ZHAN Yaoyao WANG Yi YU Yao CHEN Yang LIN Kai SONG Jian CHANG Yaqing | 2019 | Journal of Ocean University of China2019，18，5： | 1 |
| 10 | Concurrence of cat and tet Genes in Multiple Antibiotic-Resistant Bacteria Isolated from a Sea Cucumber and Sea Urchin Mariculture Farm in China显示文摘 | Hongyue Dang Linsheng Song Mingna Chen Yaqing Chang | 2006 | Microbial Ecology2006，，4： | 1 |
| 11 | Review of environmental efficiency and its influencing factors in China : 1998 - 2009 显示文摘 | Song Malin Song Yaqing An Qingxian | 2013 | Renewable and Sustainable Energy Reviews2013，20，4： | 1 |
| 12 | The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri显示文摘 | Linsheng Song Huibin Zou Yaqing Chang Wei Xu Longtao Wu | 2005 | Developmental and Comparative Immunology2005，，3： | 1 |
| 13 | The Case-Based Rea- soning Technology Based on Functional Mode显示文摘 | Li Peigang Feng Xianying Song Yaqing | 2011 | Journal of Ad-vanced Manufacturing Systems2011，10，1： | 1 |
| 14 | Comparison of single-event upset generated by heavy ion and pulsed laser显示文摘Single-event upset(SEU) is investigated using heavy ion and pulsed laser. The measured SEU cross sections of D and DICE flip-flops are compared. Measurement results indicate pulsed laser is capable of inducing similar SEU to those induced by heavy ion. 3D-TCAD simulation is performed to investigate the factors to impact pulsed laser induced SEU. Simulation results show that the beam spot size significantly impacts SEU cross sections in both low and high laser energy while the variation of the equivalent LET only impacts SEU cross sections in the low laser energy. | Bin LIANG Ruiqiang SONG Jianwei HAN Yaqing CHI Rui CHEN Chunmei HU Jianjun CHEN Yingqi MA Shipeng SHANGGUAN | 2017 | Science China(Information Sciences)2017，60，7： | 0 |
| 15 | Rapid detection of pathogenic bacteria based on a universal dual-recognition FRET sensing system constructed with aptamer-quantum dots and lectin-gold nanoparticles显示文摘The threat to public health from bacterial infections has led to an urgent need to develop simpler,faster and more reliable bacterial detection methods.In this work,we developed a universal dual-recognition based sandwich fluorescence resonance energy transfer(FRET)sensor by using specific aptamer-modified quantum dots(Aptamer-QDs)as energy donor and lectin concanavalin A(Con A)modified gold nanoparticles(Con A-AuNPs)as energy acceptor to achieve rapid and sensitive detection of Escherichia coli(E.coli)within 0.5 h.In the presence of the target E.coli,the energy donor of Aptamer-QDs and acceptor of Con A-AuNPs were close to each other,causing changes of FRET signals.Based on the constructed FRET sensor,a linear detection range of from 10^(2)cfu/mL to 2×10^(8)cfu/mL with the detection limit of 45 cfu/mL for E.coli was achieved.Furthermore,the FRET sensor was applied to detect E.coli in the milk and orange juice with the detection limit of 300 cfu/mL and 200 cfu/mL,respectively and recovery rate from 83.1%to 112.5%.The strategy holds great promise in pathogenic bacteria detection due to its rapid and sensitivity. | Yaqing Zhang Yan Liu Yun Yang Linyao Li Xiaoqi Tao Erqun Song | 2023 | Chinese Chemical Letters2023，34，8： | 0 |
| 16 | Genome Characterization of COVID-19 Lineage B.1.1.7 Detected in the First Six Patients of a Cluster Outbreak——Shenzhen City,Guangdong Province,China,May 2021显示文摘Screening for coronavirus disease 2019(COVID-19)virus,also known as SARS-CoV-2,infection every seven days was performed for high-risk populations who worked at the Yantian Port in Yantian District,Shenzhen City,Guangdong Province.On May 20,2021,an oropharyngeal swab from a 44-year-old male(Case A)tested preliminarily positive for COVID-19 by a quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)method in a third-party laboratory. | Yaqing He Peihua Niu Bo Peng Ying Sun Ziquan Lyu Renli Zhang Xiang Zhao Yang Song Yenan Feng Xiujuan Tang Dongfeng Kong Xinyi Wei Can Zhu Qingju Lu Shimin Li Xiaoliang Xiao Weiwen Liu Junjia He Junjie Xia Long Chen Xuan Zou Tiejian Feng | 2021 | China CDC weekly2021，3，25： | 0 |
| 17 | Climate change in the Hongliujing area of Lop Nur over the past 200 years revealed by the stable oxygen isotopes of Tamarix cones显示文摘The layers of Tamarix cones within sedimentary deposits in arid regions have significant chronological and paleoenvironmental implications.Here,we compare the δ^(18)O values of Tamarix cones in the Hongliujing area of Lop Nur with meteorological data for the Ruoqiang meteorological station for 1960-2019 AD.Linear regression analysis was used to reconstruct the average temperature for April and the precipitation for November in the Hongliujing area over the past 200 years.The results showed that the δ^(18)O values were significantly negatively correlated with the temperature for February,April,May,August,December,and with the annual mean temperature;significantly negatively correlated with the precipitation for February and April;significantly negatively correlated with the sunshine hours for March and May;significantly positively correlated with the sunshine hours for February,July,August,October,and December,and with the annual mean values;and significantly correlated with the relative humidity for April,July,August,September,October,and November,and with the annual mean values.Based on the δ^(18)O record of the past 200 years,the Hongliujing area experienced two warm-wet periods(1874-1932 and 2004-2019 AD)and two cold-dry periods(1832–1873 and 1933-2003 AD).Thus,the climate was characterized by alternating warm-wet and cold-dry conditions.Wavelet analysis revealed three main cycles:45 years,29 years,and 14 years. | Zhiguang LI Yaqing DONG Haoyu ZHANG Hongxiao SUN Danyang JIA Shikai SONG Yuanjie ZHAO | 2023 | Frontiers of Earth Science2023，17，4： | 0 |
| 18 | Modeling the impact of process and operation variations on the soft error rate of digital circuits显示文摘Dear editor,Process or operation variations are important factors in the soft error rate(SER)of integrated circuits[1,2].During manufacturing or other operations,inevitable process or operation variations lead to changes in the electrical parameters of transistors,which can result in sizeable shifts in the SER of integrated circuits[3].Some studies have reported that the hardness of test chips can vary by more than 20%as a result of process or operation variations[4].Therefore,it is vital to | Ruiqiang SONG Shuming CHEN Bin LIANG Yaqing CHI Jianjun CHEN | 2017 | Science China(Information Sciences)2017，60，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员