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| 1 | Medical image segmentation using improved FCM显示文摘Image segmentation is one of the most important problems in medical image processing,and the existence of partial volume effect and other phenomena makes the problem much more complex.Fuzzy C-means,as an effective tool to deal with PVE,however,is faced with great challenges in efficiency.Aiming at this,this paper proposes one improved FCM algorithm based on the histogram of the given image,which will be denoted as HisFCM and divided into two phases.The first phase will retrieve several intervals on which to compute cluster centroids,and the second one will perform image segmentation based on improved FCM algorithm.Compared with FCM and other improved algorithms,HisFCM is of much higher efficiency with satisfying results.Experiments on medical images show that HisFCM can achieve good segmentation results in less than 0.1 second,and can satisfy real-time requirements of medical image processing. | ZHANG XiaoFeng ZHANG CaiMing TANG WenJing WEI ZhenWen | 2012 | Science China(Information Sciences)2012，55，5： | 20 |
| 2 | Research on Formation Mechanisms of Hot Dry Rock Resources in China显示文摘As an important geothermal resource, hot dry rock(HDR) reserves have been studied in many countries. HDR resources in China have huge capacity and have become one of the most important resources for the potential replacement of fossil fuels. However, HDR resources are difficult to develop and utilise. Technologies for use with HDR, such as high–temperature drilling, reservoir characterisation, reservoir fracturing, microseismic monitoring and high–temperature power stations, originate from the field of oil and drilling. Addressing how to take advantage of these developed technologies is a key factor in the development of HDR reserves. Based on the thermal crustal structure in China, HDR resources can be divided into four types: high radioactive heat production, sedimentary basin, modern volcano and the inner–plate active tectonic belt. The prospective regions of HDR resources are located in South Tibet, West Yunnan, the southeast coast of China, Bohai Rim, Songliao Basin and Guanzhong Basin. The related essential technologies are relatively mature, and the prospect of HDR power generation is promising. Therefore, analysing the formation mechanisms of HDR resources and promoting the transformation of technological achievements, large–scale development and the utilisation of HDR resources can be achieved in China. | WANG Guiling LIN Wenjing ZHANG Wei LU Chuan MA Feng GAN Haonan | 2016 | Acta Geologica Sinica(English Edition)2016，90，4： | 14 |
| 3 | DIDS blocks a chloride-dependent current that is mediated by the 2B protein of enterovirus 71显示文摘 | Shiqi Xie Kai Wang Wenjing Yu Wei Lu Ke Xu Jianwei Wang Bin Ye Wolfgang Schwarz Qi Jin Bing Sun | 2011 | Cell Research2011，21，8： | 11 |
| 4 | dentification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma显示文摘 | Fei Li Zhaoyuan Fang Jian Zhang Chen Li Hongyan Liu Jufeng Xia Hongwen Zhu Chenchen auo Zhen Qin Fuming Li Xiangkun Han Yuetong Wang Yan Feng Ye Wang Wenjing Zhang Zuoyun Wang Yujuan Jin Yihua Sun Wenyi Wei Rong Zeng Haiquan Chen Hongbin Ji | 2016 | Cell Research2016，26，10： | 9 |
| 5 | A Green Paradigm for Internet of Things: Ambient Backscatter Communications显示文摘Internet of Things (IoT) has attracted extensive interest from both academia and industries, and is recognized as an ultimate infrastructure to connect everything at anytime and anywhere. The implementation of IoT generally faces the challenges from energy constraint and implementation cost. In this paper, we will introduce a new green communication paradigm, the ambient backscatter (AmBC), that could utilize the environmental wireless signals for both powering a tiny-cost device and backscattering the information symbols. Specifically, we will present the basic principles of AmBC, analyze its features and advantages, suggest its open problems, and predict its potential applications for our future IoT. | Wei Zhang Yao Qin Wenjing Zhao Minzheng Jia Qiang Liu Ruisi He Bo Ai | 2019 | China Communications2019，16，7： | 9 |
| 6 | A disintegrin and metalloproteinase with thrombospondin motif 1(ADAMTS1) expression increases in acute aortic dissection显示文摘Acute aortic dissection(AAD) is a life-threatening cardiovascular disease caused by progressive medial degeneration of the aortic wall. A disintegrin and metalloproteinase with thrombospondin motifs 1(ADAMTS1) is a recently identified extracellular metalloproteinase participating in the development of vascular disease, such as atherosclerosis. In the present study, we found that ADAMTS1 was significantly elevated in blood samples from AAD patients compared with patients with acute myocardial infarction and healthy volunteers. Based on these findings, we established an AAD model by infusing angiotensin II in older mice. AAD was successfully developed in aorta tissues, with an incidence of 42% after 14 days in the angiotensin II group. Macrophage and neutrophil infiltration was observed in the media of the aorta, and ADAMTS1 overexpression was found in the aorta by Western blot and immunohistochemistry. Double immunofluorescence staining showed the expression of ADAMTS1 in macrophages and neutrophils. Consistent with the upregulation of ADAMTS1 in aortic dissection tissues, versican(a proteoglycan substrate of ADAMTS1) was degraded significantly more in these tissues than in control aortic tissues. These data suggest that the increased expression of ADAMTS1 protein in macrophages and neutrophils that infiltrated aortic tissues may promote the progression of AAD by degrading versican. | Yanxiang Gao Wenjing Wu Changan Yu Fangming Zhong Geng Li Wei Kong Jingang Zheng | 2016 | Science China(Life Sciences)2016，59，1： | 8 |
| 7 | Characterization and Fine Mapping of a Novel Rice Narrow Leaf Mutant nal9显示文摘A narrow leaf mutant was isolated from transgenic rice(Oryza sativa L.)lines carrying a T-DNA insertion.The mutant is characterized by narrow leaves during its whole growth period,and was named nal9(narrow leaf 9).The mutant also has other phenotypes,such as light green leaves at the seedling stage,reduced plant height,a small panicle and increased tillering.Genetic analysis revealed that the mutation is controlled by a single recessive gene.A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion,so a map-based cloning strategy was employed to isolate the nal9 gene.The mutant individuals from the F2generations of a cross between the nal9 mutant and Longtepu were used for mapping.With 24 F2mutants,the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3.New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156.The nal9 gene was fnally located in a 69.3 kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking.Sequence and expression analysis showed that an ATP-dependent Clp protease proteolytic subunit gene(ClpP)was most likely to be the nal9 gene.Furthermore,the nal9 mutation was rescued by transformation of the ClpP cDNA driven by the 35S promoter.Accordingly,the ClpP gene was identifed as the NAL9 gene.Our results provide a basis for functional studies of NAL9 in future work. | Wei Li Chao Wu Guocheng Hu Li Xing Wenjing Qian Huamin Si Zongxiu Sun Xingchun Wang Yaping Fu Wenzhen Liu | 2013 | Journal of Integrative Plant Biology2013，55，11： | 8 |
| 8 | Theoretical insight into the enhanced CH_4 desorption via H_2O adsorption on different rank coal surfaces显示文摘The density functional theory was used to investigate the adsorption of CH_4 and H_2O on different rank coal surfaces.The coal rank is the dominant factor in affecting the adsorption capacity of coal.In order to better understand gas and water interaction with coal of different maturity,we developed fourteen coal models to represent the different rank coal.The interactions of CH_4 and H_2O with coal surfaces were studied and characterized by their adsorption energies,Mulliken charges and electrostatic potential surfaces.The results revealed that the interaction between coal and CH_4 was weak physical adsorption,and that the interaction between coal and H_2 O consisted of physical and chemical adsorption.Adsorption energy of coal–H_2O system was larger than that of coal–CH_4 on all rank coals,suggesting that the adsorption priority in the coal models is H_2 O > CH_4.Consequently,the injection of H_2 O into the different rank coal could effectively enhance the coal bed methane(CBM) recovery. | Yanan Zhou Wenjing Sun Wei Chu Xiaoqiang Liu Fangli Jing Ying Xue | 2016 | Journal of Energy Chemistry2016，25，4： | 8 |
| 9 | Rapid eutectic growth during free fall显示文摘Rapid eutectic growth of Sb-24%Cu alloy is realized in the drop tube during the free fall under the con-tainerless condition. Based on the analysis of crystal nuclea-tion and eutectic growth in the free fall condition, it is indicated that, with the increase of undercooling, microstruc-tural transition of Sb-24%Cu eutectic alloy proceeds from lamellar to anomalous eutectic structure. Undercoolings of 0 -154 K have been obtained in experiment. The maximum undercooling exceeds to 0.19TE. Calculated results exhibit that Cu2Sb compound is the primary nucleation phase, and that the primary Sb dendrite will grow more rapidly than the eutectic structure when undercooling is larger than 40 K. The eutectic coupled zone around Sb-24%Cu eutectic alloy leads strongly to the Cu-rich side and covers a composition range from 23.0% to 32.7%Sb. | Wenjing Yao Xiujun Han Bingbo Wei | 2002 | Chinese Science Bulletin2002，47，15： | 7 |
| 10 | Fractal dimension of coal particles and their CH_4 adsorption显示文摘We describe the fractal analysis of three differently sized coal samples(0.350-0.833 mm,0.245-0.350 mm,and 0.198-0.245 mm).The influence of fractal dimension on CH 4 adsorption capacity is investigated.The physical parameters of the samples were determined via the Brunauer-Emmett-Teller(BET) theory.A CH 4 adsorption study over the pressures range from 0 to 5 MPa was carried out with a new volumetric measurement system.The CH 4 adsorption was measured using the differently sized coal.Two fractal dimensions,D 1 and D 2 were determined over the pressure ranges from 0 to 0.5 MPa and from 0.5 to 1 MPa,using the Frenkel-Halsey-Hill(FHH) method.We conclude that the two fractal dimensions correlate with the CH 4 adsorption capacity of the coal:increasing CH 4 adsorption capacity occurs with a corresponding increase in fractal dimension.Furthermore,D 1 and D 2 are positively correlated with surface area,pore volume,and samples size.The size distribution of the samples has fractal characteristics. | Wang Chengyang Hao Shixiong Sun Wenjing Chu Wei | 2012 | International Journal of Mining Science and Technology2012，22，6： | 7 |
| 11 | Gap junction-mediated cell-to-cell communication in oral development and oral diseases: a concise review of research progress显示文摘Homoeostasis depends on the close connection and intimate molecular exchange between extracellular, intracellular and intercellular networks. Intercellular communication is largely mediated by gap junctions(GJs), a type of specialized membrane contact composed of variable number of channels that enable direct communication between cells by allowing small molecules to pass directly into the cytoplasm of neighbouring cells. Although considerable evidence indicates that gap junctions contribute to the functions of many organs, such as the bone, intestine, kidney, heart, brain and nerve, less is known about their role in oral development and disease. In this review, the current progress in understanding the background of connexins and the functions of gap junctions in oral development and diseases is discussed. The homoeostasis of tooth and periodontal tissues, normal tooth and maxillofacial development, saliva secretion and the integrity of the oral mucosa depend on the proper function of gap junctions.Knowledge of this pattern of cell–cell communication is required for a better understanding of oral diseases. With the everincreasing understanding of connexins in oral diseases, therapeutic strategies could be developed to target these membrane channels in various oral diseases and maxillofacial dysplasia. | Wenjing Liu Yujia Cui Jieya Wei Jianxun Sun Liwei Zheng Jing Xie | 2020 | International Journal of Oral Science2020，12，2： | 7 |
| 12 | Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis显示文摘Lung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders,including COVID-19.However,there are still no effective drugs to treat lung injury and fibrosis.In this study,we report the generation of clinical-grade human embryonic stem cells(hESCs)-derived immunity-and matrix-regulatory cells(IMRCs)produced under good manufacturing practice requirements,that can treat lung injury and fibrosis in vivo.We generate IMRCs by sequentially differentiating hESCs with serum-free reagents.IMRCs possess a unique gene expression profile distinct from that of umbilical cord mesenchymal stem cells(UCMSCs),such as higher expression levels of proliferative,immunomodulatory and anti-fibrotic genes.Moreover,intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury,and significantly improves the survival rate of the recipient mice in a dose-dependent manner,likely through paracrine regulatory mechanisms.IMRCs are superior to both primary UCMSCs and the FDA-approved drug pirfenidone,with an excellent efficacy and safety profile in mice and monkeys.In light of public health crises involving pneumonia,acute lung injury and acute respiratory distress syndrome,our findings suggest that IMRCs are ready for clinical trials on lung disorders. | Jun Wu Dingyun Song Zhongwen Li Baojie Guo Yani Xiao Wenjing Liu Lingmin Liang Chunjing Feng Tingting Gao Yanxia Chen Ying Li Zai Wang Jianyan Wen Shengnan Yang Peipei Liu Lei Wang Yukai Wang Liang Peng Glyn Nigel Stacey Zheng Hu Guihai Feng Wei Li Yan Huo Ronghua Jin Ng Shyh-Chang Qi Zhou Liu Wang Baoyang Hu Huaping Dai Jie Hao | 2020 | Cell Research2020，30，9： | 7 |
| 13 | Tianwen-1 MINPA observations in the solar wind显示文摘The Mars Ion and Neutral Particle Analyzer(MINPA)is one of the three scientific instruments onboard the Tianwen-1 orbiter to investigate the Martian space environment.During Tianwen-1’s transfer orbit to Mars,the MINPA was switched on to measure the solar wind ions.Here,we present the first results of the MINPA observations in the solar wind.During cruise,nearly half of the MINPA ion field-of-view(FOV)was blocked by the lander capsule;thus only the solar-wind ions with azimuthal speeds pointing towards the unblocked FOV sectors could be detected.We perform a detailed comparison of the MINPA’s solar wind observations with data from Earth-based missions when MINPA reached its count-rate peak,finding a general consistency of the ion moments between them.The blocking effect due to the lander is evaluated quantitatively under varying solar-wind velocity conditions.Despite the blocking effect,the MINPA’s solar wind measurements during the transfer orbit suggest a good performance. | AiBing Zhang LingGao Kong WenYa Li Lei Li BinBin Tang ZhaoJin Rong Yong Wei JiJie Ma YiTeng Zhang LiangHai Xie YuXian Wang JianSen He Bin Liu WenJing Wang Bin Su JiaWei Li Xu Tan Fang Wang TaiFeng Jin FuHao Qiao Peter Wurz Yan Zhu YunFei Bai YiRen Li XinBo Zhu YueQiang Sun YongLiao Zou Chi Wang | 2022 | Earth and Planetary Physics2022，6，1： | 5 |
| 14 | Nondestructive diagnostics of soluble sugar,total nitrogen and their ratio of tomato leaves in greenhouse by polarized spectra-hyperspectral data fusion显示文摘Polarized spectra-hyperspectral data fusion technique was used to estimate the soluble sugar(SS),total nitrogen(N),and their ratio(SS/N),of greenhouse tomato leaves.Fresh tomato leaves of five different growth stages(seedling,flowering,initial fruiting,mid-fruiting and picking stage)and five different nitrogen treatments(severe stress 25%,moderate stress 50%,mild stress 75%,normal 100%,and excess 150%)at every stage were collected for spectra acquisition and SS and N determination.Polarized reflectance spectra were acquired with a polarization reflectance spectrum spectro-goniophotometer system and four polarization degree features were extracted.Hyperspectral data were collected with a hyperspectral imaging system and four reflectance spectrum features and eight image features were extracted.Initially,models were built with polarization degree features,image features,and spectral features respectively.Linear and nonlinear fusion methods were comparatively used for modeling based on normalized data of the three sources.The results suggest that the performances of SS/N models are better than those of N and SS models,and the prediction capability of the Support Vector Machine(SVM)models of N and SS/N are superior to those obtained with single kind feature.This work indicates that the polarized spectrum-hyperspectral multidimensional information detecting method can feasibly judge the tomato nutrient stress conditions.Multi-features data fusion analysis technique can enhance the prediction accuracy of spectral diagnostics technology in precision agriculture. | Wenjing Zhu Jinyang Li Lin Li Aichen Wang Xinhua Wei Hanping Mao | 2020 | International Journal of Agricultural and Biological Engineering2020，13，2： | 5 |
| 15 | Exosomes with low miR-34c-3p expression promote invasion and migration of non-small cell lung cancer by upregulating integrinα2β1显示文摘Exosomes play critical roles in regulating various physiological and pathological processes,including immune stimulation,immune suppression,cardiovascular diseases,and cancers.Recent studies show that exosomes that transport specific microRNAs(miRNAs)are involved in tumor development.However,the molecular mechanism by which tumor invasion and migration are regulated by exosomes from non-small cell lung cancer(NSCLC)is not well understood.Here,we show that exosomes shuttling low levels of miR-34c-3p are involved in NSCLC progression.Our results showed that exosomes derived from NSCLC cells carrying low levels of miR-34c-3p could be transported into the cytoplasm of NSCLC cells and accelerate NSCLC invasion and migration by upregulating integrinα2β1.A luciferase assay revealed that integrinα2β1 was the direct target of miR-34c-3p,and overexpression of integrinα2β1 could promote the invasion and migration of NSCLC cells.The analysis of exosomes derived from clinical serum samples indicated that the expression of miR-34c-3p was significantly downregulated in exosomes from NSCLC patients compared with that of normal controls.A549-derived exosomes promoted NSCLC cells lung metastases in vivo.Exosomes shuttling low levels of miR-34c-3p were associated with the progression of NSCLC in vitro and in vivo.Our data demonstrate that exosomes shuttling low levels of miR-34c-3p can accelerate the invasion and migration of NSCLC by upregulating integrinα2β1.MiR-34c-3p can be a diagnostic and prognostic marker for NSCLC.High expression of integrinα2β1 is positively related to the migration and metastasis of NSCLC cells. | Wenjing Huang Yanyan Yan Yun Liu Minting Lin Jinxiang Ma Wei Zhang Jianwei Dai Jiajun Li Qiaoru Guo Hubiao Chen Bolat Makabel Hong Liu Chaoyue Su Hong Bi Jianye Zhang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 16 | Small molecules in targeted cancer therapy:advances,challenges,and future perspectives显示文摘Due to the advantages in efficacy and safety compared with traditional chemotherapy drugs,targeted therapeutic drugs have become mainstream cancer treatments.Since the first tyrosine kinase inhibitor imatinib was approved to enter the market by the US Food and Drug Administration(FDA)in 2001,an increasing number of small-molecule targeted drugs have been developed for the treatment of malignancies.By December 2020,89 small-molecule targeted antitumor drugs have been approved by the US FDA and the National Medical Products Administration(NMPA)of China.Despite great progress,small-molecule targeted anti-cancer drugs still face many challenges,such as a low response rate and drug resistance.To better promote the development of targeted anti-cancer drugs,we conducted a comprehensive review of small-molecule targeted anti-cancer drugs according to the target classification.We present all the approved drugs as well as important drug candidates in clinical trials for each target,discuss the current challenges,and provide insights and perspectives for the research and development of anti-cancer drugs. | Lei Zhong Yueshan Li Liang Xiong Wenjing Wang Ming Wu Ting Yuan Wei Yang Chenyu Tian Zhuang Miao Tianqi Wang Shengyong Yang | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 5 |
| 17 | An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo显示文摘L-glutamate,the most common excitatory neurotransmitter in the mammalian central nervous system(CNS),is associated with a wide range of neurological diseases.Because neurons in CNS communicate with each other both electrically and chemically,dualmode(electric and chemical)analytical techniques with high spatiotemporal resolution are required to better understand glutamate function in vivo.In the present study,a silicon-based implantable microelectrode array(MEA)composed of both platinum electrochemical and electrophysiological microelectrodes was fabricated using micro-electromechanical system.In the MEA probe,the electrophysiological electrodes have a low impedance of 0.018 MΩat 1 kHz,and the electrochemical electrodes show a sensitivity of 56 pAμM^(−1) to glutamate and have a detection limit of 0.5μM.The MEA probe was used to monitor extracellular glutamate levels,spikes and local field potentials(LFPs)in the striatum of anaesthetised rats.To explore the potential of the MEA probe,the rats were administered to KCl via intraperitoneal injection.K+significantly increases extracellular glutamate levels,LFP low-beta range(12–18 Hz)power and spike firing rates with a similar temporal profile,indicating that the MEA probe is capable of detecting dual-mode neuronal signals.It was concluded that the MEA probe can help reveal mechanisms of neural physiology and pathology in vivo. | Wenjing Wei Yilin Song Li Wang Song Zhang Jinping Luo Shengwei Xu Xinxia Cai | 2015 | Microsystems & Nanoengineering2015，1，1： | 4 |
| 18 | Use of catalytic wet air oxidation (CWAO) for pretreatment of high-salinity high-organic wastewater显示文摘Catalytic wet air oxidation(CWAO)coupled desalination technology provides a possibility for the effective and economic degradation of high salinity and high organic wastewater.Chloride widely occurs in natural and wastewaters,and its high content jeopardizes the efficacy of Advanced oxidation process(AOPs).Thus,a novel chlorine ion resistant catalyst Bsite Ru doped LaFe_(1-x)Ru_(x)O_(3-)δin CWAO treatment of chlorine ion wastewater was examined.Especially,LaFe_(0.85)Ru_(0.15)O_(3-δ)was 45.5% better than that of the 6%RuO_(2)@TiO_(2)(commercial carrier)on total organic carbon(TOC)removal.Also,doped catalysts LaFe_(1-x)Ru_(x)O_(3-)δshowed better activity than supported catalysts RuO_(2)@LaFeO_(3) and RuO_(2)@TiO_(2) with the same Ru content.Moreover,LaFe_(0.85)Ru_(0.15)O_(3-)δhas novel chlorine ion resistance no matter the concentration of Cl^(−) and no Ru dissolves after the reaction.X-ray diffraction(XRD)refinement,X-ray photoelectron spectroscopy(XPS),transmission electron microscope(TEM),and X-ray absorption fine structure(XAFS)measurements verified the structure of LaFe_(0.85)Ru_(0.15)O_(3-)δ.Kinetic data and density functional theory(DFT)proved that Fe is the site of acetic acid oxidation and adsorption of chloride ions.The existence of Fe in LaFe_(0.85)Ru_(0.15)O_(3-)δcould adsorb chlorine ion(catalytic activity inhibitor),which can protect the Ru site and other active oxygen species to exert catalytic activity.This work is essential for the development of chloride-resistant catalyst in CWAO. | Wenjing Sun Hongxia Lv Lei Ma Xiangdong Tan Chengyu Jin Huiling Wu Lili Chen Mengyang Liu Huangzhao Wei Chenglin Sun | 2022 | Journal of Environmental Sciences2022，34，10： | 4 |
| 19 | Pristine PN junction toward atomic layer devices显示文摘In semiconductor manufacturing,PN junction is formed by introducing dopants to activate neighboring electron and hole conductance.To avoid structural distortion and failure,it generally requires the foreign dopants localize in the designated micro-areas.This,however,is challenging due to an inevitable interdiffusion process.Here we report a brand-new junction architecture,called'layer PN junction',that might break through such limit and help redefine the semiconductor device architecture.Different from all existing semiconductors,we find that a variety of van der Waals materials are doping themselves from n-to p-type conductance with an increasing/decreasing layer-number.It means the capability of constructing homogeneous PN junctions in monolayers'dimension/precision,with record high rectification-ratio(>10^(5))and low cut-off current(<1 pA).More importantly,it spawns intriguing functionalities,like gate-switchable-rectification and noise-signal decoupled avalanching.Findings disclosed here might open up a path to develop novel nanodevice applications,where the geometrical size becomes the only critical factor in tuning charge-carrier distribution and thus functionality. | Hui Xia Man Luo Wenjing Wang Hailu Wang Tianxin Li Zhen Wang Hangyu Xu Yue Chen Yong Zhou Fang Wang Runzhang Xie Peng Wang Weida Hu Wei Lu | 2022 | Light(Science & Applications)2022，11，7： | 4 |
| 20 | Transforming growth factor-β1-induced N-cadherin drives cell–cell communication through connexin43 in osteoblast lineage显示文摘Gap junction(GJ)has been indicated to have an intimate correlation with adhesion junction.However,the direct interaction between them partially remains elusive.In the current study,we aimed to elucidate the role of N-cadherin,one of the core components in adhesion junction,in mediating connexin 43,one of the functional constituents in gap junction,via transforming growth factor-β1(TGF-β1)induction in osteoblasts.We first elucidated the expressions of N-cadherin induced by TGF-β1 and also confirmed the upregulation of Cx43,and the enhancement of functional gap junctional intercellular communication(GJIC)triggered by TGF-β1 in both primary osteoblasts and MC3T3 cell line.Colocalization analysis and Co-IP experimentation showed that N-cadherin interacts with Cx43 at the site of cell–cell contact.Knockdown of N-cadherin by siRNA interference decreased the Cx43 expression and abolished the promoting effect of TGF-β1 on Cx43.Functional GJICs in living primary osteoblasts and MC3T3 cell line were also reduced.TGF-β1-induced increase in N-cadherin and Cx43 was via Smad3 activation,whereas knockdown of Smad3 signaling by using siRNA decreased the expressions of both N-cadherin and Cx43.Overall,these data indicate the direct interactions between N-cadherin and Cx43,and reveal the intervention of adhesion junction in functional gap junction in living osteoblasts. | Yueyi Yang Wenjing Liu JieYa Wei Yujia Cui Demao Zhang Jing Xie | 2021 | International Journal of Oral Science2021，13，2： | 4 |
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