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| 1 | MdGSTF6,activated by MdMYB1,plays an essential role in anthocyanin accumulation in apple显示文摘Anthocyanins are biosynthesized on the cytosolic surface of the endoplasmic reticulum and then transported into the vacuole for storage.Glutathione S-transferases(GSTs)are considered to be responsible for the transport of anthocyanins into the vacuole.However,the regulatory mechanisms of GSTs in plants are still unclear.Here,we performed a genome-wide analysis and identified 69 GST genes in apple.The expression of MdGSTF6 was positively correlated with the anthocyanin content(r=0.949)during‘Yanfu 8’fruit development.The overexpression of MdGSTF6 in the Arabidopsis thaliana tt19 mutant resulted in seedlings of 35S::MdGSTF6-GFP/tt19 that could accumulate anthocyanin and rescue its phenotype,suggesting that MdGSTF6 was an anthocyanin transporter.The silencing of MdGSTF6 affected anthocyanin accumulation in apple fruit.Moreover,the knockdown of MdGSTF6 by RNA interference in cultured‘Gala’seedlings inhibited anthocyanin accumulation.The interaction experiments showed that MdMYB1 could bind directly to the MdGSTF6 promoter to transcriptionally activate its expression.Collectively,our results demonstrate that MdGSTF6 encodes an important GST transporter of anthocyanins in apple fruit and provide evidence for the associated regulatory mechanisms.Therefore,MdMYB1 can not only regulate anthocyanin synthesis,but also control the transport of anthocyanin in apples.This information may be useful for further clarifying the regulation of anthocyanin transport in apple. | Shenghui Jiang Min Chen Naibo He Xiaoliu Chen Nan Wang Qingguo Sun Tianliang Zhang Haifeng Xu Hongcheng Fang Yicheng Wang Zongying Zhang Shujing Wu Xuesen Chen | 2019 | Horticulture Research2019，6，1： | 16 |
| 2 | Anelasticity of twinned CuO nanowires显示文摘CuO nanowires (NW ) 的机械行为被调查由在 situ 传播电子显微镜学。在压缩期间, NW 展出了与高机械的应力联系的高弯曲的能力。有趣地,滞弹性一致地在压力版本以后被观察。进一步的调查显示滞弹性对 CuO NW 内在,尽管 electronbeam 照耀加速形状恢复被证明有能力。机制基于联系双胞胎的原子的合作运动被建议说明这现象。结果提供卓见进 CuO NW 的机械性质,它是为抑制系统的 nanoscale 的有希望的材料。 | Huaping Sheng He Zheng Fan Cao Shujing Wu Lei Li Chun Liu Dongshan Zhao Jianbo Wang | 2015 | Nano Research2015，8，11： | 9 |
| 3 | Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple显示文摘In plants,the vesicle fusion process plays a vital role in pathogen defence.However,the importance of the vesicle fusion process in apple ring rot has not been studied.Here,we isolated and characterised the apple syntaxin gene MdSYP121.Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection;this increased tolerance was correlated with salicylic acid(SA)synthesis-related and signalling-related gene transcription.In contrast,overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes.In addition,the results of RNA sequencing(RNA-Seq)and quantitative real-time PCR(qRT-PCR)assays suggested that MdSYP121 plays an important role in responses to oxidation–reduction reactions.Silencing MdSYP121 in apple calli enhanced the expression levels of reactive oxygen species(ROS)-related genes and the activity of ROS-related enzymes.The enhanced defence response status in MdSYP121-RNAi lines suggests that syntaxins are involved in the defence response to B.dothidea.More importantly,we showed that MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex with MdSNAP33,and the complex may participate in regulating resistance to B.dothidea.In conclusion,by regulating the interaction of SA pathway and oxidation–reduction process,MdSYP121 can influence the pathogen infection process in apple. | Xiaowen He Yanhong Huo Xiuxia Liu Qianqian Zhou Shouqian Feng Xiang Shen Baohua Li Shujing Wu Xuesen Chen | 2018 | Horticulture Research2018，5，1： | 3 |
| 4 | Implantable CMOS neuro-stimulus chip for visual prosthesis显示文摘A prototype chip with 2×2 pixels for implanting in blind patients affected by outer retinal degeneration is presented in this paper.This visual prosthesis chip imitates the degenerated photoreceptor cells,senses the incident light and stimulates the remaining healthy layers of retina or optic nerve.Each pixel integrates photodiode and stimulus pulse generator,converting the illumination on the eyes into 3-bit resolution bi-phasic current pulses.On-chip charge cancellation modules are used to discharge each electrode site for tissue safety.The prototype chip is designed and fabricated in 0.18-μm N-well CMOS (complementary metal oxide semiconductor) 1P6M Mix-signal process,with a ±2.5 V dual voltage supply.The functionality of the fabricated chip is demonstrated on anesthetized rabbits.Neural responses in visual cortex are successfully evoked by the neuro-stimulus chip through an on-board trigger interface and flexible electrode. | ZHANG Xu PEI WeiHua HUANG BeiJu WANG ShuJing GUAN Ning GUO Kai WANG Yu GUI Qiang CHEN Jin WANG Kai WU HuiJuan LI XiaoXin LI Kai CHEN HongDa | 2011 | Science China(Information Sciences)2011，54，4： | 3 |
| 5 | Generation of polarization-entangled photon pairs in a cold atomic ensemble显示文摘我们在一个冷原子整体报导纠缠极化的光子对的试验性的代。一个单身者司烧光子和光子纺纱波浪刺激同时经由散布的自发的拉曼被创造。纺纱波浪刺激然后被变换成反 -- 司烧经由一个 electromagnetic-induced-transparency 读物过程的光子。在 Stokes 和反之间的测量跨关联的功能 -- 司烧为二直角的极化的光子是分别地, 75 和 74 ~ 60/s 的光子对在代评估的 ~ 。基于如此的关联,我们获得纠缠极化的光子对, S 是其贝尔参数 ?=? 2.77 ?? ??? ??????? € 楎 ??? 猼'T 资 ? ?? ￣ ? 猼'T?? ?吗?? | Yuelong Wu Shujing Li Wei Ge Zhongxiao Xu Long Tian Hai Wang | 2016 | Science Bulletin2016，61，4： | 3 |
| 6 | The role of circadian rhythm in breast cancer显示文摘The circadian rhythm is an endogenous time keeping system shared by most organisms. The circadian clock is comprised of both peripheral oscillators in most organ tissues of the body and a central pacemaker located in the suprachiasmatic nucleus (SCN) of the central nervous system. The circadian rhythm is crucial in maintaining the normal physiology of the organism including, but not limited to, cell proliferation, cell cycle progression, and cellular metabolism; whereas disruption of the circadian rhythm is closely related to multi-tumorigenesis. In the past several years, studies from different fields have revealed that the genetic or functional disruption of the molecular circadian rhythm has been found in various cancers, such as breast, prostate, and ovarian. In this review, we will investigate and present an overview of the current research on the influence of circadian rhythm regulating proteins on breast cancer. | Shujing Li Xiang Ao Huijian Wu | 2013 | Chinese Journal of Cancer Research2013，25，4： | 3 |
| 7 | Construction of WO_(3)/Ti-doped WO_(3)bi-layer nanopore arrays with superior electrochromic and capacitive performances显示文摘Crystalline WO_(3)/Ti-doped WO_(3)bi-layer nanopore arrays were constructed by the template synthesis of a WO_(3)nanopore layer modified by a magnetron sputtering of an amorphous Ti-doped/WO_(3)layer.The obtained bi-layer nanopore array shows a remarkable electrochromic performance with large dual-band optical modulation in both visible(VIS)and near infrared(NIR)regions(optical modulation of over 70%in the wavelength range from 600 to 1600 nm)and the fast response speed(coloring for 3.4 s and bleaching for 6.6 s).In addition,the bi-layer WO_(3)/Ti-doped WO_(3)nanopore array also present superior energy-storage properties(areal capacitance of 44.0 mF cm−2 and good rate capability),better than that of titanium-free thin films.The special bifunctional characteristics of electrochromism and pseudocapacitance can be ascribed to the large specific surface area provided by the architectural design,rich ion channels in the amorphous layer as well as proper titanium doping,which bestows the bi-layer nanopore array a great potential in clean energy applications. | Yingdi Shi Shujing Liu Yong Zhang Mingjun Sun Kai Tang Jiewu Cui Xia Shu Yan Wang Jiaqin Liu Yucheng Wu | 2019 | Tungsten2019，1，3： | 2 |
| 8 | Development of potent promoters that drive the efficient expression of genes in apple protoplasts显示文摘Protoplast transient expression is a powerful strategy for gene functional characterization,especially in biochemical mechanism studies.We herein developed a highly efficient transient expression system for apple protoplasts.The abilities of the Arabidopsis thaliana and Malus domestica ubiquitin-10(AtUBQ10 and MdUBQ10)promoters to drive the expression of multiple genes were compared with that of the CaMV 35S promoter,and the results revealed that the AtUBQ10 and MdUBQ10 promoters were more efficient in apple protoplasts.With this system,we demonstrated that active AtMKK7ac could activate MAPK6/3/4 signaling cascades,which further regulated MdWRKY33 phosphorylation and stability in apple.Furthermore,the ligand-induced interaction between the immune receptor AtFLS2 and the coreceptor AtBAK1 was reconstituted in apple protoplasts.We also found that the stability of the bacterial effector AvrRpt2 was regulated by feedback involving auxin and the immune regulator RIN4.The system established herein will serve as a useful tool for the molecular and biochemical analyses of apple genes. | Xianpu Wang Lili Xu Xiuxia Liu Li Xin Shujing Wu Xuesen Chen | 2021 | Horticulture Research2021，8，1： | 2 |
| 9 | Creation of fragrant sorghum by CRISPR/Cas9显示文摘Sorghum,the fifth largest cereal crop,has high value as a staple food and raw material for liquor and vinegar brewing.Due to its high biomass and quality,it is also used as the second most planted silage resource.No fragrant sorghums are currently on the market.Through CRISPR/Cas9-mediated knockout of Sb BADH2,we obtained sorghum lines with extraordinary aromatic smell in both seeds and leaves.Animal feeding experiments showed that fragrant sorghum leaves were attractable.We believe this advantage will produce great value in the sorghum market for both grain and whole biomass forage. | Dan Zhang Sanyuan Tang Peng Xie Dekai Yang Yaorong Wu Shujing Cheng Kai Du Peiyong Xin Jinfang Chu Feifei Yu Qi Xie | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 10 | Solution-processable precursor route for fabricating ultrathin silica film for high performance and low voltage organic transistors显示文摘Silica is one of the most commonly used materials for dielectric layer in organic thin-film transistors due to its excellent stability, excellent electrical properties, mature preparation process, and good compatibility with organic semiconductors. However, most of conventional preparation methods for silica film are generally performed at high temperature and/or high vacuum. In this paper, we introduce a simple solution spin-coating method to fabricate silica thin film from precursor route, which possesses a low leakage current, high capacitance, and low surface roughness. The silica thin film can be produced in the condition of low temperature and atmospheric environment. To meet various demands, the thickness of film can be adjusted by means of preparation conditions such as the speed of spin-coating and the concentration of solution. The p-type and n-type organic field effect transistors fabricated by using this film as gate electrodes exhibit excellent electrical performance including low voltage and high performance. This method shows great potential for industrialization owing to its characteristic of low consumption and energy saving, time-saving and easy to operate. | Shujing Guo Zhongwu Wang Zeyang Xu Shuguang Wang Kunjie Wu Shufeng Chen Zongbo Zhang Caihong Xu Wenfeng Qiu Liqiang Li | 2017 | Chinese Chemical Letters2017，28，11： | 1 |
| 11 | A water - soluble two - photon photopolymerization initiation system: Methylated - β - cyclodextrin complex of xanthene dye/aryliodonium salt 显示文摘 | Shujing Li Lidong Li Feipeng Wu ErjianWang | 2009 | Journal of Photochemistry and Photobiology A: Chemistry2009，203，： | 1 |
| 12 | Photoreactive inclusion complex of aryhodonium salt encapsulated by methylated - β- cyclodextrin 显示文摘 | Shujing Li Feipeng Wu Erjian Wang Lidong Li | 2008 | Journal of Photochemistry and Photobiology A : Chemistry2008，197，： | 1 |
| 13 | Chinese emotional words in patients with major depressive disorder during a subliminal Stroop task An event-related potential study显示文摘Patients with major depressive disorder (MDD) develop a negative cognitive bias, but how they respond to information in Chinese emotional words is unclear. Here we used a Stroop paradigm with subliminal Chinese emotional words to explore the event-related potential components of abnormal emotional processing in patients with MDD. The correct rate was similar in MDD and normal control groups, but MDD reaction time was longer than the normal controls, especially to the negative and neutral stimuli. In N270, repeated-measure analysis of variance demonstrated a significant main effect of the relation electrode and valence on peak amplitude and interactions between valence and electrode site. The peak amplitudes of the three kinds of words were different in the two groups (positive > negative > neutral). The topography of the difference waves indicated that the difference distributed in the frontal and left parietal-temporal sites across the scalp. In N400, there was a significant main effect of the relation electrode and valence on peak amplitude, and the latency showed a main effect of the electrode and an interaction between electrode and group. The amplitudes induced by type of words were significantly different from each other in both groups (positive > negative > neutral). The topography of the difference waves indicated that the effect of relation type was primarily at left and right frontal and central and left parietal-temporal regions. Both MDD patients and normal controls exhibited significant emotional Stroop effects during the processing of positive/negative Chinese emotional words. MDD patients showed interference in emotional stimuli in early cognitive processing that induced psychological resource intervention during late emotional information processing. | Daxing Wu Shujing Xu Huifang Yin | 2010 | Neural Regeneration Research2010，5，16： | 1 |
| 14 | Determi-nation of the Thermal Loading in Laserdiode-pumped Erbium-ytterbium-codoped Phosphate Glass Micro-chip Laser显示文摘 | Song Feng Liu Shujing Wu Zhaohui | | 0，，： | 1 |
| 15 | Determination of the Thermal Loading in Laserdiode-pumped Erbium-ytterbium-codoped Phosphate Glass Microchip Laser显示文摘 | Feng Song Shujing Liu Zhaohui Wu | 2007 | Opt Soc Am B2007，24，9： | 1 |
| 16 | Determination of the Thermal Loading in Laserdiodepumped Erbium-ytterbium-codoped Phosphate Glass Mierochip Laser显示文摘 | SONG Feng LIU Shujing WU Zhaohui | 2007 | Journal of the Optical Society of America B2007，24，9： | 1 |
| 17 | Extracellular ATP-induced NO production and its dependence on membrane Ca2 + flux in Salvia miltiorrhiza hairy roots显示文摘 | WU Shujing WU Jianyong | 2008 | Journal of Experiment Botany2008，59，14： | 1 |
| 18 | Chlorophyllide-a Oxygenase 1(OsCAO1) Over-Expression Affects Rice Photosynthetic Rate and Grain Yield显示文摘Leaf color and photosynthesis are important factors for rice growth and development.Hence,improving the photosynthetic rate is an effective approach for increasing rice yield.We isolated a gene,chlorophyllide-a oxygenase 1(OsCAO1),which characterized a rice near-isogenic line named fgl(faded green leaf). | HU Ping MA Jie KANG Shujing LI Sanfeng WU Xianmei ZENG Longjun LU Caolin HE Rui HE Huiying SHANG Lianguang RAO Yuchun ZHU Xudong XIONG Guosheng QIAN Qian GUO Longbiao WANG Yuexing | 2023 | Rice science2023，30，2： | 1 |
| 19 | Real-time PCR method for detection of the transgenic rice event TT51 - 1 显示文摘 | WU Gang WU Yuhua NIE Shujing | 2010 | Food Chemistry2010，119，： | 1 |
| 20 | Extracellular and intracellular infection of Botryosphaeria dothidea and resistance mechanism in apple cells显示文摘Apple ring rot,which is caused by Botryosphaeria dothidea,severely affects apple production.The mechanisms employed in apple cells against B.dothidea remain unknown.In this research,the pathogen infection mode and the relationship between cell death and disease resistance in‘Fuji’/B.dothidea interaction pathosystem were investigated.By using transmission electron microscopy(TEM),our research showed that the pathogen infects apple cells both intracellularly and extracellularly.However,compared with that in immature fruit,the incidence of hyphae in the interior of mature apple fruit cells increased dramatically,suggesting that cell wall-mediated penetration resistance could be important in apple resistance against B.dothidea.TEM ultrastructural characterization identified the nuclear morphology of programmed cell death induction in both apple fruit and callus cells under B.dothidea infection.Overexpression of MdVDAC2(MDP0000271281),which encodes an outer-membrane localized anion channel protein in mitochondria,significantly promoted cell death under B.dothidea infection and simultaneously inhibited pathogen infection,suggesting that cell death represents a disease resistance mechanism in apple against B.dothidea infection.Furthermore,BdCatalase(KAF4307763),a cytochromeP450 family protein BdCYP52A4(KAF4300696),and subtilisin-domain containing proteinswere identified fromB.dothidea-secreted proteins,which suggested the potential involvement of active oxygen species and phytoalexins in combating B.dothidea infection and triggering or dampening apple resistance.Collectively,our research suggested that cell wall-mediated penetration resistance,programmed cell death machinery and microbial effector-interrelated signaling were among strategies recruited in apple to combat B.dothidea.The current research laid the foundation for further investigations into resistance mechanisms in apple. | Li Xin Rui Zhang Xianpu Wang Xiuxia Liu Yicheng Wang Peiran Qi Lishuang Wang Shujing Wu Xuesen Chen | 2023 | Horticultural Plant Journal2023，9，2： | 1 |
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