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| 1 | A human circulating immune cell landscape in aging and COVID-19显示文摘Age-associated changes in immune cells have been linked to an increased risk for infection.However,a global and detailed characterization of the changes that human circulating immune cells undergo with age is lacking.Here,we combined scRNA-seq,mass cytometry and sCATAC-seq to compare immune cell types in peripheral blood collected from young and old subjects and patients with COVID-19.We found that the immune cell landscape was reprogrammed with age and was characterized by T cell polarization from naive and memory cells to effector,cytotoxic,exhausted and reg-ulatory cells,along with increased late natural killer cells,age-associated B cells,inflammatory monocytes and age-associated dendritic cells.In addition,the expression of genes,which were implicated in coron-avirus susceptibility,was upregulated in a cell subtype-specific manner with age.Notably,COVID-19 promoted age-induced immune cell polarization and gene expression related to inflammation and cellular senes-cence.Therefore,these findings suggest that a dysreg-ulated immune system and increased gene expression associated with SARS-CoV-2 susceptibility may at least partially account for COVID-19 vulnerability in the elderly. | Yingfeng Zheng Xiuxing Liu Wenqing Le Lihui Xie He Li Wen Wen Si Wang Shuai Ma Zhaohao Huang Jinguo Ye Wen Shi Yanxia Ye Zunpeng Liu Moshi Song Weiqi Zhang Jing-Dong J.Han Juan Carlos lzpisua Belmonte Chuanle Xiao Jing Qu Hongyang Wang Guang-Hui Liu Wenru Su | 2020 | Protein & Cell2020，11，10： | 10 |
| 2 | Hepatoprotective ability of tetramethylpyrazine produced by Bacillus amyloliquefaciens显示文摘Tetramethylpyrazine(TTMP),an important aroma compound,was produced by Bacillus amyloliquefaciens XJB-104 with distillers’grains as raw material.The yield of TTMP under optimized fermentation procedure was 3176.52 mg/L.TTMP in the fermentation broth was purified and the purity(>99%)was measured by gas chromatography-mass spectrometry.Furthermore,the hepatoprotective activity of TTMP in ethanol-water system was evaluated by biochemical indicators of liver injury,parameters of antioxidant defense system and inflammatory response,and liver histopathological assessment in mice.Ethanol treatment increased serum levels of alanine aminotransferase,aspartate aminotransferase,alkaline phos-phatase and lactate dehydrogenase,suggesting liver damage.Both high and low dose of TTMP significantly decreased levels of these indicators.Furthermore,the reduction in the activities or concentrations of superoxide dismutase,catalase,reduced glutathione and malondialdehyde in liver tissue caused by ethanol was significantly alleviated by TTMP.Increased inflammatory cytokines including transcription factor,tumor necrosis factor,interleukin-1beta,interleukin,macrophage chemoattractant protein,inducible nitric oxide synthase and cyclooxygenase were also suppressed by TTMP treatment.It is concluded that TTMP in ethanol-water system has potential liver-protective activity,especially when ethanol is consumed at low doses for short periods of time. | Wenqing Zhang Guanru Si Zhiming Rao Shuai Zong Jinglei Li Xian Zhang Chuanqiang Gao Zhou Ping Ming Ye | 2021 | Systems Microbiology and Biomanufacturing2021，1，2： | 4 |
| 3 | Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene显示文摘The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases(TALENs) technology to generate Leptin receptor(Lepr) knockout rats on the Sprague Dawley(SD) genetic background. Through direct injection of in vitro transcribed m RNA of TALEN pairs into SD rat zygotes, somatic mutations were induced in two of three resulting pups. One of the founders carrying bi-allelic mutation exhibited early onset of obesity and infertility. The other founder carried a chimeric mutation which was efficiently transmitted to the progenies. Through phenotyping of the resulting three lines of rats bearing distinct mutations in the Lepr locus, we found that the strains with a frame-shifted or premature stop codon mutation led to obesity and metabolic disorders. However, no obvious defect was observed in a strain with an in-frame 57 bp deletion in the extracellular domain of Lepr. This suggests the deleted amino acids do not significantly affect Lepr structure and function. This is the first report of generating the Lepr mutant obese rat model in SD strain through a reverse genetic approach. This suggests that TALEN is an efficient and powerful gene editing technology for the generation of disease models. | Yuting Chen Wenqing Lu Na Gao Yi Long Yanjiao Shao Meizhen Liu Huaqing Chen Shixin Ye Xueyun Ma Mingyao Liu Dali Li | 2017 | Science China(Life Sciences)2017，60，2： | 4 |
| 4 | Combined detection and subclass characteristics analysis of CTCs and CTECs by SE-iFISH in ovarian cancer显示文摘Objective:Hematogenous metastasis is essential for the progression of ovarian cancer(OC),and circulating tumor cells(CTCs)are part of the metastatic cascade.However,the detection rate of CTC is low due to the use of less sensitive detection methods.Therefore,this study aimed to detect CTCs and circulating tumorigenic endothelial cells(CTECs)in patients with OC using subtraction enrichment and immunostaining and fluorescence in situ hybridization(SE-iFISH).Methods:We enrolled a total of 56 subjects,including 20 OC patients and 36 ovarian benign tumor patients.CTCs and CTECs were captured by subtraction enrichment(SE)and counted and classified according to immunofluorescence staining of tumor markers(TMs)carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)combined with fluorescence in situ hybridization(iFISH)of chromosome 8(Chr8)aneuploidy.The diagnostic value and subtype characteristics of CTCs and CTECs were investigated.Results:The detection rate of CTCs by SE-iFISH was high.Compared with CA125 and HE4,Chr8 aneuploidy was the major identification feature of CTC.CTC counts in OC were statistically higher than those in benign groups.CTC and CTEC with≥pentaploidy were detected in both groups,illustrating the poor diagnostic value of CTC or CTEC.Distributions of triploid and tetraploid CTC subtypes were significantly different,and combined detection of triploid and tetraploid CTCs showed the best diagnostic value.In contrast,the distribution of CTECs in the OC and benign groups had no statistically significant difference.Small CTCs accounted for over 1/3 of the total CTC count.We also found that small CTCs and CTECs primarily comprised triploid cells,while large CTCs and CTECs mainly comprised pentaploidy and beyond.Conclusions:The application of SE-iFISH offered a more comprehensive understanding of heterogeneous CTCs and CTECs in OC.Analysis of subclass characteristics of the CTCs and CTECs according to Chr8 aneuploidy and cell size may broaden their potential clinical utility and deepen mechanistic studies in OC. | Hongyan Cheng Shang Wang Wenqing Luan Xue Ye Sha Dou Zhijian Tang Honglan Zhu Peter Ping Lin Yi Li Heng Cui Xiaohong Chang | 2021 | Chinese Journal of Cancer Research2021，33，2： | 3 |
| 5 | Photocatalytic Conversion of Carbon Dioxide with Water into Methane: Platinum and Copper(I) Oxide Co‐catalysts with a Core–Shell Structure显示文摘 | Qingge Zhai Shunji Xie Wenqing Fan Qinghong Zhang Yu Wang Weiping Deng Ye Wang | 2013 | Angew Chem Int Ed2013，，22： | 1 |
| 6 | TPC in the leaves of 116 sweet potato ( Ipomoea batatas L.) varieties and Pushu 53 leaf extracts显示文摘 | Wenqing Xu Lixiang Liu Bing Hu Yi Sun Hong Ye Daifu Ma Xiaoxiong Zeng | 2010 | Journal of Food Composition and Analysis2010，，6： | 1 |
| 7 | Active-layer evolution and (CH3NH3)3Bi2l9-based solar substrate efficiency improvement of cell on TiO2-deposited ITO显示文摘 | Xinqian Zhang Gang Wu Zhuowei Gu Bing Guo Wenqing Liu Shida Yang Tao Ye Chen Chen Weiwei Tu Hongzheng Chen | 2016 | Nano Research2016，9，10： | 1 |
| 8 | Nanoeomposites of TiO2 and Reduced Graphene Oxide as Efficient Photocata- lysts for Hydrogen Evolution显示文摘 | Fan Wenqing Lai Qinghua Zhang Qinghong Wang Ye | 2011 | The Journal of Physical Chemistry C2011，115，10： | 1 |
| 9 | Heritable expansion of the genetic code in mouse and zebrafish显示文摘 | Yuting Chen Ji Ma Wenqing Lu Meilin Tian Marion Thauvin Chonggang Yuan Michel Volovitch Qian Wang Jeff Holst Mingyao Liu Sophie Vriz Shixin Ye Lei Wang Dali Li | 2017 | Cell Research2017，27，2： | 1 |
| 10 | Discussion on the causes of 21 cases of显示文摘 | Huang Guoping Liu Huifang Ye Wenqing | | 0，，14： | 1 |
| 11 | Activation and Coreceptor Expression of T Lymphocytes in HIV/AIDS Patients of China显示文摘 | Hong Shang Zining Zhang Yongjun Jiang Xiaoxu Han Yanan Wang Min Zhang Xiaohui Ye Ying Liu Yingying Diao Di Dai Wenqing Geng | 2005 | Journal of Clinical Immunology2005，，1： | 1 |
| 12 | 1α,25(OH)2D3对斑马鱼脂肪组织中脂肪酸氧化的促进显示文摘文章简介1α,25(OH)2D3被认为是哺乳类动物的矿物质代谢的重要调控激素,但其在动物脂肪组织代谢中的精确机制却不够清晰。肝细胞微小体中的细胞色素P450酶2R1(CYP2R1)是活化维生素D3的第一步,即25位的羟基化的羟基化酶,此功能在脊椎动物中是保守。 | Xuyan Peng Guohui Shang Wenqing Wang Xiaowen Chen Qiyong Lou Gang Zhai Dongliang Li Zhenyu Du Yali Ye Xia Jin Jiangyan He Yi Zhang 殷战 | 2018 | 科学新闻2018，0，4： | 0 |
| 13 | Semi-Empirical Formulas for Nuclear Charge Radii | Wang, Jiansong Shen, Wenqing Feng, Jun Ye, Wei | 1997 | Chinese journal of nuclear physics1997，，1： | 0 |
| 14 | Defect-mediated Rashba engineering for optimizing electrical transport in thermoelectric BiTeI显示文摘The Rashba effect plays a vital role in electronic structures and related functional properties.The strength of the Rashba effect can be measured by the Rashba parameter α_(R);it is desirable to manipulate α_(R) to control the functional properties.The current work illustrates how α_(R) can be systematically tuned by doping,taking BiTeI as an example. | Xin Li Ye Sheng Lihua Wu Shunbo Hu Jiong Yang David J.Singh Jihui Yang Wenqing Zhang | 2020 | npj Computational Materials2020，，1： | 0 |
| 15 | Violation of the T^(−1) Relationship in the Lattice Thermal Conductivity of Mg_(3)Sb_(2) with Locally Asymmetric Vibrations显示文摘Most crystalline materials follow the guidelines of T^(-1) temperature-dependent lattice thermal conductivity(κ_(L))at elevated temperatures.Here,we observe a weak temperature dependence ofκL in Mg_(3)Sb_(2),T^(-0:48) from theory and T-0:57 from measurements,based on a comprehensive study combining ab initio molecular dynamics calculations and experimental measurements on single crystal Mg_(3)Sb_(2).These results can be understood in terms of the so-called“phonon renormalization”effects due to the strong temperature dependence of the interatomic force constants(IFCs).The increasing temperature leads to the frequency upshifting for those low-frequency phonons dominating heat transport,and more importantly,the phononphonon interactions are weakened.In-depth analysis reveals that the phenomenon is closely related to the temperature-induced asymmetric movements of Mg atoms within MgSb_(4) tetrahedron.With increasing temperature,these Mg atoms tend to locate at the areas with relatively low force in the force profile,leading to reduced effective 3^(rd)-order IFCs.The locally asymmetrical atomic movements at elevated temperatures can be further treated as an indicator of temperature-induced variations of IFCs and thus relatively strong phonon renormalization.The present work sheds light on the fundamental origins of anomalous temperature dependence of κ_(L) in thermoelectrics. | Yifan Zhu Yi Xia Yancheng Wang Ye Sheng Jiong Yang Chenguang Fu Airan Li Tiejun Zhu Jun Luo Christopher Wolverton GJeffrey Snyder Jianjun Liu Wenqing Zhang | 2020 | Research2020，，1： | 0 |
| 16 | Polaron interfacial entropy as a route to high ther moelectric perfor mance in DAE-doped PEDOT:PSS films显示文摘Enhancing the thermoelectric transport properties of conductive polymer materials has been a long-term challenge,in spite of the success seen with molecular doping strategies.However,the strong coupling between the thermopower and the electrical conductivity limits thermoelectric performance.Here,we use polaron interfacial occupied entropy engineering to break through this intercoupling for a PEDOT:PSS(poly(3,4-ethylenedioxythiophene)-poly(4-styrenesulfonate))thin film by using photochromic diarylethene(DAE)dopants coupled with UV-light modulation.With a 10-fold enhancement of the thermopower from 13.5μV K^(-1) to 135.4μV K^(-1) and almost unchanged electrical conductivity,the DAE-doped PEDOT:PSS thin film achieved an extremely high power factor of 521.28μW m^(-1) K^(-2) from an original value of 6.78μW m^(-1) K^(-2).The thermopower was positively correlated with the UV-light intensity but decreased with increasing temperature,indicating resonant coupling between the planar closed DAE molecule and PEDOT.Both the experiments and theoretical calculations consistently confirmed the formation of an interface state due to this resonant coupling.Interfacial entropy engineering of polarons could play a critical role in enhancing the thermoelectric performance of the organic film. | Jiajia Zhang Caichao Ye Genwang Wei Liang Guo Yuhang Cai Zhi Li Xinzhi Wu Fangyi Sun Qikai Li Yupeng Wang Huan Li Yuchen Li Shuaihua Wang Wei Xu Xuefeng Guo Wenqing Zhang Weishu Liu | 2024 | National Science Review2024，11，3： | 0 |
| 17 | Incidence and risk factors of early transient intraocular pressure elevation after canaloplasty for primary open-angle glaucoma显示文摘Glaucoma is one of the most common optic neuropathies,featuring progressive retinal ganglion cell damage and visual field loss(Tham et al.,2014;Xu et al.,2020).Currently,the only effective treatment for this condition is the reduction of intraocular pressure(IOP)(Palmberg,2001;Heijl et al.,2002).Canaloplasty is a proven bleb-independent surgery with good efficacy and safety profiles in primary open-angle glaucoma(POAG)(Gołaszewska et al.,2021).However,early transient postoperative IOP elevation has been reported in up to 30%of cases(Riva et al.,2019),similar to that commonly observed in other internal drainage glaucoma surgeries such as implantation using iStent(0%–21.0%),CyPass(10.8%),and Hydrus(4.8%–6.5%)(Lavia et al.,2017). | Lijuan XU Xinyao ZHANG Yang CAO Yin ZHAO Juan GU Wenqing YE Xiaojie WANG Jinxin LI Ruiyi REN Yuanbo LIANG | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，4： | 0 |
| 18 | Analysis on Spatial Pattern of Land Use Based on Fractal Theory:A Case Study of a Southwest Town显示文摘Based on GIS,RS technology and fractal theory,this paper analyzes land use type of a southwest town in 2010. It obtains fractal model,fractal dimension and stability index of land use types,which will provide favorable reference for healthy social and economic development of this town and scientific decision making for rational control of land resource. | Haixia LUO Kai LUO Lusheng YE Wenqing CHEN Zhengshan LI | 2013 | Asian Agricultural Research2013，5，7： | 0 |
| 19 | Novel Way to Determine Reaction Plane Dispersion and Its Test to Reaction of 10.6 MeV/u ^(84)Kr on ^(27)Al | Shen Wenqing~(1,2) Ye Wei~1 Ma Yugang~(1,2) Feng Jun~(1,2) Wang Jiansong~11 Shanghai Institute of Nuclear Research,the Chinese Academy of Sciences,P.O.Box 800204,Shanghai 2018002 CCAST(World Laboratory),P.O.Box 8730,Beijing 100080 | 1997 | Chinese journal of nuclear physics1997，，2： | 0 |
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