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| 1 | Expression Analysis of miRNAs and Highly-expressed Small RNAs in Two Rice Subspecies and their Reciprocal Hybrids显示文摘Heterosis,or hybrid vigor,is the phenomenon whereby progeny of two inbred lines exhibit superior agronomic performance compared with either parent.We analyzed the expression of miRNAs and highly expressed small RNAs (defined according to Solexa sequencing results) in two rice (Oryza sativa) subspecies (japonica cv.Nipponbare and indica cv.93-11) and their reciprocal hybrids using microarrays.We found that of all the 1141 small RNAs tested,140 (12%,140 of 1141) and 157 (13%,157 of 1141) were identified being significantly differentially expressed in two reciprocal hybrids,respectively.All possible modes of action,including additive,high-and low-parent,above high-and below low-parent modes were exhibited.Both F1 hybrids showed non-additive expression patterns,with downregulation predominating.Interestingly,15 miRNAs displayed stark opposite expression trends relative to midparent in reciprocal hybrids.Computational prediction of targets of differentially expressed miRNAs showed that they participated in multifaceted developmental pathways,and were not distinguishable from the targets of non-differentially expressed miRNAs.Together,our findings reveal that small RNAs play roles in heterosis and add a new layer in the understanding and exploitation of molecular mechanisms of heterosis. | Fangfang Chen Guangming He Hang He Wei Chen Xiaopeng Zhu Manzhong Liang Liangbi Chen Xing Wang Deng | 2010 | Journal of Integrative Plant Biology2010，52，11： | 10 |
| 2 | A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding显示文摘Watermelon,Citrullus lanatus,is the world's third largest fruit crop.Reference genomes with gaps and a narrow genetic base hinder functional genomics and genetic improvement of watermelon.Here,we report the assembly of a telomere-to-telomere gap-free genome of the elite watermelon inbred line G42 by incorporating high-coverage and accurate long-read sequencing data with multiple assembly strategies.All 11 chromosomes have been assembled into single-contig pseudomolecules without gaps,representing the highest completeness and assembly quality to date.The G42 reference genome is 369321829 bp in length and contains 24205 predicted protein-coding genes,with all 22 telomeres and 11 centromeres characterized.Furthermore,we established a pollen-EMS mutagenesis protocol and obtained over 200000M1 seeds from G42.In a sampling pool,48 monogenic phenotypic mutations,selected from 223M1and 78 M2 mutants with morphological changes,were confirmed.The average mutation density was 1 SNP/1.69Mband1 indel/4.55 Mb per M1 plant and 1SNP/1.08Mb and 1 indel/6.25 Mb per M2 plant.Taking advantage of the gap-free G42 genome,8039 mutations from 32 plants sampled from M1 and M2 families were identified with 100%accuracy,whereas only 25% of the randomly selected mutations identified using the 97103v2 reference genome could be confirmed.Using this library and the gap-free genome,two genes responsible for elongated fruit shape and male sterility(CiMs1)were identified,both caused by a single basechange from G to A.The validated gap-free genome and its EMS mutation library provide invaluable resources for functional genomics and genetic improvement of watermelon. | Yun Deng Shoucheng Liu Yilin Zhang Jingsheng Tan Xiaopeng Li Xiao Chu Binghua Xu Yao Tian Yudong Sun Bosheng Li YunbiXu Xing Wang Deng Hang He Xingping Zhang | 2022 | Molecular Plant2022，15，8： | 9 |
| 3 | LSECtin on tumor-associated macrophages enhances breast cancer sternness via interaction with its receptor BTN3A3显示文摘Macrophages have bee n suggested to con tribute to con structing a cancer stem cell(CSC)niche.However,whether and how macrophages regulate the activity of CSCs through juxtacrine signaling are poorly understood.Here we report LSECtin,a transmembrane protein highly expressed on tumor-associated macrophages(TAMs),enhances sternness of breast cancer cells(BCCs).We identified BTN3A3,a B7 family member with previously unknown functions as the receptor for LSECtin on BCCs responsible for stemness-promoting effect of LSECtin.In mice bearing human tumor xenografts,either macrophage-specific ablation of LSECtin or silencing of BTN3A3 in BCCs decreased CSC frequency and tumor growth.Admixture of LSECtin-positive macrophages increased the tumorigenic activity of BCCs dependent on BTN3A3.Disruption of the LSECtin-BTN3A3 axis with BTN3A3-Fc or anti-BTN3A3 mAb has a therapeutic effect on breast cancer.These findings define a juxtacrine signaling mechanism by which TAMs promote cancer stemness.Targeting this axis in the CSC niche may provide potential therapies to breast cancer. | Di Liu Qian Lu Xing Wang Jun Wang Ning Lu Zefei Jiang Xiaopeng Hao Jianbin Li Jing Liu Pengbo Cao Guilin Peng Yuandong Tao Dianyuan Zhao Fuchu He Li Tang | 2019 | Cell Research2019，29，5： | 8 |
| 4 | A collinear tandem time-of-flight mass spectrometer for infrared photodissociation spectroscopy of mass-selected ions显示文摘An apparatus based on collinear tandem time-of-flight mass spectrometer has been designed for the measurement of infrared photodissociation spectroscopy of mass-selected ions in the gas phase.The ions from a pulsed laser vaporization supersonic ion source are skimmed and mass separated by a Wiley-McLaren time-of-flight mass spectrometer.The ion of interest is mass selected,decelerated and dissociated by a tunable IR laser.The fragment and parent ions are reaccelerated and mass analyzed by the second time-of-flight mass spectrometer.A simple new assembly integrated with mass gate,deceleration and reacceleration ion optics was designed,which allows us to measure the infrared spectra of mass selected ions with high sensitivity and easy timing synchronization. | WANG GuanJun CHI ChaoXian XING XiaoPeng DING ChuanFan ZHOU MingFei | 2014 | Science China Chemistry2014，57，1： | 2 |
| 5 | The Study of Visible Implant Elastomer(VIE)Tag on Small-sized Chinese Shrimp(Fenneropenaeus chinensis)显示文摘[Objective] The paper was to prove the feasibility of using visible implant elastomer( VIE) tags to mark Chinese shrimp of about 1 cm in length.[Method]In June 2014,2 856 Chinese shrimps with an average length of 1. 14 cm were tagged with red VIE. After temporary rearing for one day,2 321 tagged shrimps were cultured in the same pond with 60 000 untagged shrimps in this experiment. In predation test,spottedtail gobies( Synechogobius ommaturus) were selected as the predator,and the predation rate of spottedtail gobies on small-sized Chinese shrimps was measured. [Result]On September 21,2014,206 kg of Chinese shrimp was harvested in the pond. There were 389 tagged shrimps,with an average body weight of 16. 84 g,and an average length of 11. 15 cm. The untagged shrimps had an average body weight of 17. 08 g,and average length of 11. 23 cm. The results indicated that there was no significant difference in growth between the tagged and untagged shrimps. The survival rate of the tagged shrimps was 2. 7% lower than the untagged ones. Although the fluorescent tag dispersed and the position changed,the tag was still distinct both under ultraviolet light and to the naked eye. The predation test revealed that the fluorescent tag of shrimps did not increase the predation rate by spottedtail gobies. [Conclusion]Red VIE tagging is feasible in Chinese shrimps. There was no significant difference in predation rate between untagged and VIE tagged shrimps. | Hao Xiaopeng Liu Haiying Zhang Guosheng Liu Qing Yin Zengqiang Wang Xiuli Xing Kun Liu Qi Ma Jun Chen Lei | 2017 | Animal Husbandry and Feed Science2017，9，1： | 1 |
| 6 | Herbal medicine in the treatment of COVID-19 based on the gut-lung axis显示文摘Respiratory symptoms are most commonly experienced by patients in the early stages of novel coronavirus disease 2019 (COVID-19). However, with a better understanding of COVID-19, gastrointestinal symptoms such as diarrhea, nausea, and vomiting haveattracted increasing attention. The gastrointestinal tract may be a target organ of severe acute respiratory syndrome coronavirus2 (SARS-CoV-2) infection. The intestinal microecological balance is a crucial factor for homeostasis, including immunity andinflammation, which are closely related to COVID-19. Herbal medicine can restore intestinal function and regulate the gut florastructure. Herbal medicine has a long history of treating lung diseases from the perspective of the intestine, which is called thegut-lung axis. The physiological activities of guts and lungs influence each other through intestinal flora, microflora metabolites,and mucosal immunity. Microecological modulators are included in the diagnosis and treatment protocols for COVID-19. Inthis review, we demonstrate the relationship between COVID-19 and the gut, gut-lung axis, and the role of herbal medicine intreating respiratory diseases originating from the intestinal tract. It is expected that the significance of herbal medicine in treatingrespiratory diseases from the perspective of the intestinal tract could lead to new ideas and methods for treatment. | Qiaoyu He Yumeng Shi Qian Tang Hong Xing Han Zhang Mei Wang Xiaopeng Chen | 2022 | Acupuncture and Herbal Medicine2022，2，3： | 1 |
| 7 | Gut lumen-targeted oral delivery system for bioactive agents to regulate gut microbiome显示文摘Gut microbiome(GM)is closely related to the overall health status for the human being.The dysfunction of microbiome can lead to many diseases such as inflammation,cancer and neurodegenerative disease.Therefore,it's of great importance to develop effective strategy to regulate GM.Gut lumen-targeted oral delivery system(GLT-ODS)has been extensively investigated and widely used in food science and engineering in these years,due to the targeted delivery property,controlled release profile,high biocompatibility and enhanced bioavailability of cargos.Herein,we comprehensively summarized the current advances in GLT-ODS for bioactive agent.Specifically,we systematically summarize the GLT-ODS for pre-agents(prebiotics and probiotics)and anti-agents(antibiotics and bacteriophages)according to the type of cargos.Through in-depth discussion of representative researches,we refined the limitations of existing research in carrier material and target selection,and drew a blueprint for the future technological research and development.We believe that GLT-ODS will become a safe,efficient,simple and precise GM management strategy for improvement of health and shine in the development of precision food and pharmaceutical engineering in future. | Junyu Liu Xiaobin Li Xiaopeng Zhang Runming Wang Sanyang Han Peiwu Qin Xin-Hui Xing Canyang Zhang | 2022 | Journal of Future Foods2022，2，4： | 1 |
| 8 | Application of contrast-enhanced ultrasound in the diagnosis of solid pancreatic lesions—A comparison of conventional ultrasound and contrast-enhanced CT显示文摘 | Zhihui Fan Ying Li Kun Yan Wei Wu Shanshan Yin Wei Yang Baocai Xing Xiaoting Li Xiaopeng Zhang | 2013 | European Journal of Radiology2013，，： | 1 |
| 9 | Community poverty and inequality in western China: A tale of three villages in Guizhou Province 显示文摘 | Xing Li Fan Shenggen Luo Xiaopeng Zhang Xiaobe | 2009 | China Eco- nomic Review2009，20，2： | 1 |
| 10 | Application Effect of Clinical Pathway Teaching Method in Clinical Teaching of Cardiology显示文摘Objective:To study the clinical effect of applying clinical pathway teaching method in clinica1 teaching of cardiology.Methods:This paper verifies and calculates that 50 interns working in cardiology department in our hospita1 from October 2016 to October 2019 are divided into groups and comparea in the form of double-blind method.1he rererence group(n=25)uses traditional teachingmethods,and the experimental group(n=25)uses clinical pathway teaching method.The satisfaction degree,teaching quality,theoretical assessment results,operation assessment and comprehensive assessment results of interns in the experimental group and the reference group are compared.Results:The satisfaction degree,theoretical examination result,operation examinationt comprehensive examination result,improvement of team spirit,professiona1 quality,improvemen of professional ethics,standardized diagnosis and treatment,improvement of clinical problem handling ability,stimulation of learning interest and cultivation of clinical thinking of the interns in the experimental group were compared with those of the reference group(P<0.05),showing the value of statistica1 comparison and demonstration between data indexes.Conclusion:The application of clinical path-teaching method in clinical teaching of cardiology can improve the clinical teaching effect and teaching quality. | Xiaopeng Wu Hongmei Shangguan Leisen Han Tongchao Xing | 2020 | Journal of Clinical and Nursing Research2020，4，4： | 1 |
| 11 | Structures and properties the lead-doped carbon clusters PbCn/PbCn^+/PbCn(n=10)显示文摘 | Guoliang Li Xiaopeng Xing Zichao Tang | 2003 | J Chem Phys2003，118，15： | 1 |
| 12 | Assembly of 3D-printed Ti scaffold and free vascularized fibula using a customized Ti plate for the reconstruction of mandibular defects显示文摘Introduction Mandibular segmental defects result in significant cosmetic and functional deficiencies.Meanwhile,the reconstruction of both the contour and function of the mandible is a challenging task.At present,autologous vascularized fibula transplantation is the most common method to reconstruct a mandible with long-span defects[1]. | Chiyang Zhong Yixuan Zhao Hongyu Xing Qingguo Lai Runqi Xue Tianxiang Song Xiaopeng Tang Kaiwen Zhu Yanwei Deng | 2022 | Bio-Design and Manufacturing2022，5，2： | 0 |
| 13 | Mass spectrometric study of Ag's clustering ability with O,S and Se显示文摘Samples Ag/O/S, Ag/O/Se and Ag/S/Se were ablated directly by laser to produce clusters. The detection was performed with a time-of-flight mass spectrometer (TOF MS). The cluster ions produced had the following distribution: Ag/S binary cluster ions for sample Ag/O/S, Ag/Se binary cluster ions for sample Ag/O/Se, and Ag/Se binary cluster ions and Ag/S/Se ternary cluster ions as the main products for sample Ag/S/Se. Laser double ablation reactor was used to study the reaction between Ag/S(Se, O) binary clusters and Se(S) homoclusters. The experiments show that Se clusters can replace S and O in Ag/S and Ag/O clusters while S clusters can replace O in Ag/O clusters. This fact indicates the sequence of the clustering ability of Ag presented as follows: Ag/Se > Ag/S > Ag/O, which was also proved by theoretical calculation. The reason of this tendency was considered to be the different abilities of the elements’ polarization, which acts on the stability of clusters. | Xiaopeng Xing Xia Zhang Xiang Zhao Zhen Gao | 2001 | Chinese Science Bulletin2001，46，7： | 0 |
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