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| 1 | Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis显示文摘Lung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders,including COVID-19.However,there are still no effective drugs to treat lung injury and fibrosis.In this study,we report the generation of clinical-grade human embryonic stem cells(hESCs)-derived immunity-and matrix-regulatory cells(IMRCs)produced under good manufacturing practice requirements,that can treat lung injury and fibrosis in vivo.We generate IMRCs by sequentially differentiating hESCs with serum-free reagents.IMRCs possess a unique gene expression profile distinct from that of umbilical cord mesenchymal stem cells(UCMSCs),such as higher expression levels of proliferative,immunomodulatory and anti-fibrotic genes.Moreover,intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury,and significantly improves the survival rate of the recipient mice in a dose-dependent manner,likely through paracrine regulatory mechanisms.IMRCs are superior to both primary UCMSCs and the FDA-approved drug pirfenidone,with an excellent efficacy and safety profile in mice and monkeys.In light of public health crises involving pneumonia,acute lung injury and acute respiratory distress syndrome,our findings suggest that IMRCs are ready for clinical trials on lung disorders. | Jun Wu Dingyun Song Zhongwen Li Baojie Guo Yani Xiao Wenjing Liu Lingmin Liang Chunjing Feng Tingting Gao Yanxia Chen Ying Li Zai Wang Jianyan Wen Shengnan Yang Peipei Liu Lei Wang Yukai Wang Liang Peng Glyn Nigel Stacey Zheng Hu Guihai Feng Wei Li Yan Huo Ronghua Jin Ng Shyh-Chang Qi Zhou Liu Wang Baoyang Hu Huaping Dai Jie Hao | 2020 | Cell Research2020，30，9： | 7 |
| 2 | Commuting Involutions with Fixed Point Set of Constant Codimension | Yanying Wang Zhende Wu Yanhong Ding, Department of Mathematics, Hebei Normal University, Shijiazhuang 050016, P. R. China | 1999 | Acta Mathematica Sinica,English Series1999，15，2： | 6 |
| 3 | The effects of asymmetric potential vorticity forcing on the instability of South Asia High and Indian summer monsoon onset显示文摘Based on the theory of potential vorticity(PV),the unstable development of the South Asia High(SAH)due to diabatic heating and its impacts on the Indian Summer Monsoon(ISM)onset are studied via a case diagnosis of 1998.The Indian Summer Monsoon onset in 1998 is related to the rapidly strengthening and northward moving of a tropical cyclone originally located in the south of Arabian Sea.It is demonstrated that the rapid enhancement of the cyclone is a consequence of a baroclinic development characterized by the phase-lock of high PV systems in the upper and lower troposphere.Both the intensification of the SAH and the development of the zonal asymmetric PV forcing are forced by the rapidly increasing latent heat released from the heavy rainfall in East Asia and South East Asia after the onsets of the Bay of Bengal(BOB)monsoon and the South China Sea(SCS)monsoon.High PV moves southwards along the intensified northerlies on the eastern side of the SAH and travels westwards on its south side,which can reach its northwest.Such a series of high PV eddies are transported to the west of the SAH continuously,which is the main source of PV anomalies in the upper troposphere over the Arabian Sea from late spring to early summer.A cyclonic curvature on the southwest of the SAH associated with increasing divergence,which forms a strong upper tropospheric pumping,is generated by the anomalous positive PV over the Arabian Sea on 355 K.The cyclone in the lower troposphere moves northwards from low latitudes of the Arabian Sea,and the upper-layer high PV extends downwards and southwards.Baroclinic development thus occurs and the tropical low-pressure system develops into an explosive vortex of the ISM,which leads to the onset of the ISM.In addition,evolution of subtropical anticyclone over the Arabian Peninsula is another important factor contributing to the onset of the ISM.Before the onset,the surface sensible heating on the Arabian Peninsula is very strong.Consequently the subtropical anticyclone which dominated the Arabian Sea in spring retreats westwards to the Arabian Peninsula and intensifies rapidly.The zonal asymmetric PV forcing develops gradually with high PV eddies moving southwards along northerlies on the eastern side of the anticyclone,and a high PV trough is formed in the middle troposphere over the Arabian Sea,which is favorable to the explosive barotropic development of the tropical cyclone into the vortex.Results from this study demonstrate that the ISM onset,which is different from the BOB and the SCS monsoon onset,is a special dynamical as well as thermodynamic process occurring under the condition of fully coupling of the upper,middle,and lower tropospheric circulations. | ZHANG YaNi WU GuoXiong LIU YiMin GUAN Yue | 2014 | Science China Earth Sciences2014，57，2： | 5 |
| 4 | Comparison of benzothiazole-based dyes for sensitive DNA detection显示文摘For efficient and quantitative DNA detection,fluorescence staining is the most often explored approach,which relies on non-covalent binding of dyes with double stranded DNA(dsDNA).Ethidium bromide(EB)is the most classic DNA stain,but suffers from its high carcinogenicity.A series of less toxic alternatives were developed,many of which contain the core structure of the benzothiazole ring.However,the relationship between the structure and the DNA detection performance was not illustrated.Herein,five benzothiazole dyes,namely thiazole orange,SYBR Green I,Pico Green,SYBR Safe,and thioflavine-T,were compared for DNA detection through direct fluorescence and gel electrophoresis,with particular focus on the structure-performance relationship.It turned out that SYBR Green I is currently the best choice for DNA detection.The results in this work may be useful for future DNA-staining dye developments. | Yanying Wang Ronghui Zhou Wenxue Liu Chao Liu Peng Wu | 2020 | Chinese Chemical Letters2020，31，11： | 4 |
| 5 | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function显示文摘Soy consumption has been associated with potential health benefits in reducing chronic diseases.These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides.Thus,more studies are required to identify these bioactive peptides,and elucidate their biological mechanisms of action.In the present study,a novel peptide iglycin was purifi ed from soybean seeds with a molecular mass of 3.88 k Da.Thereafter,iglycin reduced fasting blood glucose and restored insulin sensitivity of C57 BL/6 J mice on a high-fat diet with increased phosphorylation of insulin receptor substrate 1(IRS1)and AKT in adipose tissue.Furthermore,it improved glucose uptake,induced translocation of intracellular GLUT4 to plasma membrane and activation of insulin signaling in adipocytes under insulin-resistant condition.In addition,it decreased reactive oxygen species production,lipid peroxidation and inhibited adipocyte apoptosis with improved mitochondrial function as evidenced by up-regulation of succinate dehydrogenase activity,mitochondrial membrane potential and intracellular ATP store.These data suggested that iglycin ameliorated insulin resistance via activation of insulin signaling,which was associated with inhibition of oxidative stress,adipocyte apoptosis,and improvement of mitochondrial function. | Yinghuan Wu Ran Zhao Minxia Li Huiyun Li Zhengwang Chen Yanying Zhao | 2022 | Food Science and Human Wellness2022，11，6： | 3 |
| 6 | Associated risk factors in the early stage of diabetic retinopathy显示文摘Background:To investigate the retinal capillary density(RCD)of the macula using optical coherence tomography angiography(OCT-A)in type 2 diabetic patients and to further determine the association with risk factors.Methods:A total of 212 eyes from 212 subjects were recruited;subjects included diabetics with no retinopathy(NDR,n=90 eyes),diabetics with mild retinopathy DR(MDR,n=36 eyes),and healthy participants(Control,n=86 eyes).All participants underwent OCT-A scanning.RCD was quantified by superficial and deep retinal capillary layers(SRCL and DRCL)from OCT-A images.Results:RCD in SRCL and DRCL was lower in NDR(P<0.001)as well as in MDR(P<0.001)when compared with control eyes.Diabetic patients were subdivided according to individual risk factors,complications related to diabetes,and hyperglycemia.Diabetic patients showed lower RCD in both the SRCL and DRCL when compared with healthy controls.Diabetics with age>55y,HbA1c>7%had significantly reduced DRCL(P<0.05)when compared with the other group of diabetics(age<55y,HbA1c<7%).Diabetics with a blood urea nitrogen(BUN)>8.2 mmol/L had significantly reduced SRCL and DRCL when compared to the other group of diabetics.Conclusions:Risk factors including older age,higher level of HbA1c,LDL-C and BUN,were associated with lower RCDs found in type 2 diabetic patients with and without mild DR by OCT-A.The impairment of retinal capillary by OCT-A may play a key role in the early monitoring of management in diabetes. | Fan Tan Qi Chen Xiran Zhuang Chaoming Wu Yanying Qian Yuanyuan Wang Jianhua Wang Fan Lu Meixiao Shen Yingzi Li | 2019 | Eye and Vision2019，6，1： | 2 |
| 7 | The Asparagine-Rich Protein NRP Facilitates the Degradation of the PP6-type Phosphatase FyPP3 to Promote ABA Response in Arabidopsis显示文摘 | Tong Zhu Yanying Wu Xiaotong Yang Wenli Chen Qingqiu Gong Xinqi Liu | 2018 | Molecular Plant2018，11，2： | 2 |
| 8 | Degradation of 2,4-DCP by the Immobilized Laccase on the Carrier of Fe304@SiO2-NH2显示文摘在这份报纸,在 Fe3O4@SiO2-NH2 nanoparticles 上使不能调动的 laccase 被 glutaraldehyde cross-linking 方法成功地准备。laccase 催化并且使 laccase 不能调动的 2,4-dichlorophenol (2,4-DCP ) 的降级被执行。关于降级效率的最佳的条件也被讨论,它包括反应时间, pH 价值,温度, 2,4-DCP 和 laccase 的集中。当 laccase 被 crosslinking 与 glutaraldehyde 在 Fe3O4@SiO2-NH2 搬运人上使不能调动时,稳定性和重复显著地被改进。由使不能调动的 laccase 的 2,4-DCP 的移动效率在操作的六个周期以后仍然是超过 59% 。 2,4-DCP 的降级是一阶的反应, laccase 催化并且使 laccase 不能调动的 2,4-DCP 的激活精力是 51.93 kJ 敶?湡?硥楨楢整?楨桧猠汥'虡N楶祴愠摮猠湥楳楴楶祴映牯???潩獮?獁愠???浡汰晩敩?汦潵敲'諟?牰扯??獴映畬牯獥散据?灳'虪箾溪e潨敷???潦摬攠桮湡散敭瑮椠?桴?湩整獮瑩?景琠敨猠杩慮?瑡??渠?湯戠湩楤杮眠瑩?桴?牃??慂敳?湯琠敨映畬牯獥散据?楴牴瑡潩?灳'虪?湡?潊??汰瑯愠慮祬楳?戠湩楤杮洠摯?景丠児?眠瑩?牃?眠獡瀠潲潰敳??畬牯獥散据?湩整獮瑩?慷?楬敮牡眠瑩?潣据湥牴瑡潩?景???慣楴湯椠?? | Huan Weiwei Yang Yuxiang Wu Bin Yuan Hongming Zhang Yani Liu Xiangnong | 2012 | Chinese Journal of Chemistry2012，30，12： | 2 |
| 9 | Study on a series of main-chain liquid-crystalline ionomers containing sulfonate groups 显示文摘 | Zhi Junge Zhang Baoyan Wu Yanying | 2001 | Journal of Applied Polymer Science2001，81，9： | 1 |
| 10 | Pressure-induced amorphous zeolitic imidazole frameworks with reduced toxicity and increased tumor accumulation improves therapeutic efficacy In vivo显示文摘Zeolitic Imidazole Frameworks(ZIFs)are widely applied in nanomedicine for their high drug loading,suitable pore size,pH-responsive drug release,and so on.However,fast drug release during circulation,unexpected toxicity to mice major organs,undesirable long-term accumulation in the lung and even death currently hinder their in vivo biomedical applications.Herein,we report an amorphous ZIF-8(aZIF-8)with high loading of 5-Fu through pressure-induced amorphization.This nano-system avoids early drug release during circulation and provides tumor microenvironment-responsive drug release with improved in vitro cell viability,and survival rate in in vivo evaluations as compared to ZIF-8.Furthermore,aZIF-8 shows longer blood circulation and lower lung accumulation than ZIF-8 at same injected doses.Less drug release during circulation,longer blood circulation,and better biocompatibility of aZIF-8/5-Fu significantly improves its therapeutic efficacy in ECA-109 tumorbearing mouse,and result in 100%survival rate over 50 days after treatment.Therefore,aZIF-8 with favorable biocompatibility and long blood circulation is expected to be a promising nano-system for efficacious cancer therapy in vivo. | Zhenqi Jiang Yanying Li Zhenni Wei Bo Yuan Yinjie Wang Ozioma Udochukwu Akakuru Yong Li Juan Li Aiguo Wu | 2021 | Bioactive Materials2021，6，3： | 1 |
| 11 | Effect of salt concentrations on the displacement adsorption enthalpies of denatures protein folding at hydrophobic surface显示文摘 | GENG Xinpeng WU Yani SONG Jirong | 2006 | J Thetm Anal Calor2006，85，3： | 1 |
| 12 | Research progress on the substrate for metal-organic framework(MOF) membrane growth for separation显示文摘During the last decade, metal-organic frameworks(MOFs) have been applied in various fields due to their unique chemical and functional advantages. One of the widespread research hotspots is MOF-based membranes for separations, specifically continuous defect-free MOF membranes, which are usually grown on porous substrates. The substrate not only serves as the MOF layer support but also has a great influence on the membrane fabrication process and the final separation performance of the resultant membrane. In this review, we mainly introduce the progress focused on the substrates for MOF membranes fabrication. The substrate modifications and seeding methods aimed at synthesizing highquality MOF membranes are also summarized systematically. | Wufeng Wu Xilu Hong Jiang Fan Yanying Wei Haihui Wang | 2023 | Chinese Journal of Chemical Engineering2023，56，4： | 1 |
| 13 | Analysis of interfacial thermal stresses of chip-substrate structure 显示文摘 | Feng Yanying Wu Linzhi | 2001 | International Journal of Solids and Structures2001，38，： | 1 |
| 14 | Chemieal elemental characteristics of biomass fuels in China显示文摘 | LIAOC WU C YANY | 2004 | Biomass & Bioenergy2004，，27： | 1 |
| 15 | The binding epitope of sintilimab on PD-1 revealed by AbMap显示文摘PD-1 plays an important role as an immune checkpoint.Sintilimab is a newly approved PD-1 antibody for cancer immunotherapy with an unknown binding epitope on PD-1.In this study,to elucidate the molecular mechanism by which sintilimab blocks PD-1 activation,we applied Antibody binding epitope Mapping(AbMap)to identify the binding epitope of sintilimab.An epitope was successfully identified,i.e.SLAPKA,aa 127-132.By constructing a series of point mutations,the dominant residues S127,L128,A129,P130,and A132 of PD-1 were further validated by western blot analysis,biolayer interferometry,and flow cytometry.Structural analysis showed that the epitope is partially within the binding interface of PD-1 and PD-L1,and this epitope also partially overlaps with that of nivolumab and pembrolizumab.These results demonstrate that sintilimab can attenuate PD-1 activation by directly competing with the interaction between PD-1 and PD-L1 through binding with the key residues of the FG loop on PD-1.This study also demonstrates the high efficiency and accuracy of AbMap for determining the binding epitope of therapeutic antibodies. | Mingliang Ma Huan Qi Chuansheng Hu Zhaowei Xu Fanlin Wu Nan Wang Danyun Lai Yang Li Hainan Zhang Hewei Jiang Qingfeng Meng Shujuan Guo Yani Kang Xiaodong Zhao Hua Li Sheng-ce Tao | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 1 |
| 16 | Growth and characterization of Mg(OH) 2 film on magnesium alloy AZ31显示文摘 | Yanying Zhu Guangming Wu Yun-Hong Zhang Qing Zhao | 2011 | Applied Surface Science2011，，14： | 1 |
| 17 | Ectoplc expression of the rice lumazine synthase gene contributes to defense responses in transgenic tobacco 显示文摘 | Wu Tingquan Guo An Zhao Yanying | 2010 | Phytopathology2010，100，6： | 1 |
| 18 | Body-centered-cubic structure and weak anharmonic phonon scattering in tungsten显示文摘It was recently found that the anharmonic phonon–phonon scattering in tungsten is extremely weak at high frequencies,leading to a predominance of electron-phonon scattering and consequently anomalous phonon transport behaviors.In this work,we calculate the phonon linewidths of W along high-symmetry directions from first principles.We find that the weak phonon–phonon scattering can be traced back to two factors.The first is the triple degeneracy of the phonon branches at the P and H points,a universal property of elemental body-centered-cubic(bcc)structures.The second is a relatively isotropic character of the phonon dispersions.When both are met,phonon–phonon scattering rates must vanish at the P and H points.The weak phonon–phonon scattering feature is also applicable to Mo and Cr.However,in other elemental bcc substances like Na,the isotropy condition is violated due to the unusually soft character of the lower transverse acoustic phonon branch along the Γ-N direction,opening emission channels and leading to much stronger phonon–phonon scattering.We also look into the distributions of electron meanfree paths(MFPs)at room temperature in tungsten,which can help engineer the resistivity of nanostructured W for applications such as interconnects. | Yani Chen Jinlong Ma Shihao Wen Wu Li | 2019 | npj Computational Materials2019，，1： | 0 |
| 19 | Influences of Echinacea Polysaccharide on Secretion of IL-8mRNA by LPS-injured IEC-6 Cells显示文摘[Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8m RNA by LPS-injured IEC-6 cells,in order to figure out the mechanism of EPS on injured cells. [Method]Total RNA was extracted with TRIzon reagent. IL-8 m RNA was amplified by RT-PCR and detected by agarose gel electrophoresis. Furthermore,electrophoresis and image was analyzed. [Result] The 50 μg/m L EPS could partially inhibit IL-8 m RNA level produced by LPS-stimulated IEC-6; with the increasing concentration of EPS(200 and 500 μg/m L),the inhibitory effect against IL-8 m RNA gradually enhanced. IEC-6 was pretreated by 50,100,200 and 500 μg/m L EPS for 24 h,then stimulated by 10 μg/m L LPS for 1 and 4 h,respectively. RT-PCR analysis showed that the expression of IL-8 m RNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8m RNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at 1 h. [Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 m RNA by LPS-stimulated cells,and the inhibition of EPS was dependent to concentration and time. | Shi Qiumei Zhang Yanying Jia Qinghui Zhao Huiyuan Fu Yanfang Zhang Zhiqiang Wu Tonglei Hu Tiefeng An Jiwei | 2017 | Animal Husbandry and Feed Science2017，9，2： | 0 |
| 20 | Identification and optimization of peptide inhibitors to block VISTA/PSGL-1 interaction for cancer immunotherapy显示文摘Developing new therapeutic agents for cancer immunotherapy is highly demanding due to the low response ratio of PD-1/PD-L1 blockade in cancer patients.Here,we discovered that the novel immune checkpoint VISTA is highly expressed on a variety of tumor-infiltrating immune cells,especially myeloid derived suppressor cells(MDSCs)and CD8^(+)T cells.Then,peptide C1 with binding affinity to VISTA was developed by phage displayed bio-panning technique,and its mutant peptide VS3 was obtained by molecular docking based mutation.Peptide VS3 could bind VISTA with high affinity and block its interaction with ligand PSGL-1 under acidic condition,and elicit anti-tumor activity in vivo.The peptide DVS3-Pal was further designed by D-amino acid substitution and fatty acid modification,which exhibited strong proteolytic stability and significant anti-tumor activity through enhancing CD8^(+)T cell function and decreasing MDSCs infiltration.This is the first study to develop peptides to block VISTA/PSGL-1 interaction,which could act as promising candidates for cancer immunotherapy. | Xiaoshuang Niu Menghan Wu Guodong Li Xiuman Zhou Wenpeng Cao Wenjie Zhai Aijun Wu Xiaowen Zhou Shengzhe Jin Guanyu Chen Yanying Li Jiangfeng Du Yahong Wu Lu Qiu Wenshan Zhao Yanfeng Gao | 2023 | Acta Pharmaceutica Sinica B2023，13，11： | 0 |
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