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| 1 | AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants显示文摘AtbHLH29 of Arabidopsis, encoding a bHLH protein, reveals a high similarity to the tomato FER which is proposed as a transcriptional regulator involved in controlling the iron deficiency responses and the iron uptake in tomato. For identification of its biological functions, AtbHLH29 was introduced into the genome of the tomato FER mutant T3238fer mediated by Agrobacterium tumefaciencs. Transgenic plants were regenerated and the stable integration of AtbHLH29 into their genomes was confirmed by Southern hybridization. Molecular analysis demonstrated that expression of the exogenous AtbHLH29 of Arabidopsis in roots of the FER mutant T3238fer enabled to complement the defect functions of FER. The transgenic plants regained the ability to activate the whole iron deficiency responses and showed normal growth as the wild type under iron-limiting stress. Our transformation data demonstrate that AtbHLH29 is a functional ortholog of the tomato FER and can completely replace FER in controlling the effective iron acquisition in tomato. Except of iron, FER protein was directly or indirectly involved in manganese homeostasis due to that loss functions of FER in T3238fer resulted in strong reduction of Mn content in leaves and the defect function on Mn accumulation in leaves was complemented by expression of AtbHLH29 in the transgenic plants. Identification of the similar biological functions of FER and AtbHLH29, which isolated from two systematically wide-diverged “strategy I” plants, suggests that FER might be a universal gene presented in all strategy I plants in controlling effective iron acquisition system in roots. | You Xi YUAN Juan ZHANG Dao Wen WANG Hong Qing LING | 2005 | Cell Research2005，15，8： | 22 |
| 2 | Amplitude-Integrated Electroencephalography Predicts Outcome in Patients with Coma After Acute Brain Injury显示文摘Prognostication of coma patients after brain injury is important, yet challenging. In this study, we evaluated the predictive value of amplitude-integrated electroencephalography(a EEG) for neurological outcomes in coma patients. From January 2013 to January 2016, 128 coma patients after acute brain injury were prospectively enrolled and monitored with a EEG. The 6-month neurological outcome was evaluated using the Cerebral Performance Category Scale. a EEG monitoring commenced at a median of 7.5 days after coma onset. Continuous normal voltage predicted a good 6-month neurological outcome with a sensitivity of 93.6% and specificity of 85.2%. In contrast, continuous extremely low voltage, burst-suppression, or a flat tracing was correlated with poor 6-month neurological outcome with a sensitivity of 76.5% and specificity of 100%. In conclusion, a EEG is a promising predictor of 6-month neurological outcome for coma patients after acute brain injury. | Wendong You Qilin Tang Xiang Wu Junfeng Feng Qing Mao Guoyi Gao Jiyao Jiang | 2018 | Neuroscience Bulletin2018，34，4： | 15 |
| 3 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 4 | Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation显示文摘Anthocyanins are a class of products of plant secondary metabolism and are responsible for tubers color in potato.The biosynthesis of anthocyanins is a complex Researchbiological process,in which multiple genes are involved including structural genes and regulatory genes.In this study,StAN11,a WD40-repeat gene,was cloned from potato cultivar Chieftain(Solanum tuberosum L.).StAN11(HQ599506)contained no intron and its open reading frame(ORF)was 1,029 bp long,encoding a putative protein of 342 amino acids.In order to verify its role in anthocyanin biosynthesis,StAN11 was inserted behind the CaMV-35S promoter of pCMBIA1304 and the recombination vector was introduced into the potato cultivar Désirée plants by Agrobacterium-mediated transformation.The color of transgenic tuber skin was significantly deepened,compared to the wild-type control,which was highly consistent with the accumulation of anthocyanin and expression of StAN11 in transgenic lines tuber skin.Further analysis on the expression of Flavonone-3-hydroxylase(F3H),Dihydroflavonol reductase(DFR),Anthocyanidin synthase(ANS),and Flavonoid 3-O-glucosyl transferase(3GT)in transgenic plants revealed that only DFR was upregulated.This result suggested that StAN11 regulated anthocyanin biosynthesis in potato by controlling DFR expression and accumulation of anthocyanin could be increased through overexpression of StAN11 in the tubers with the genetic background of anthocyanin biosynthesis. | Wang Li Bing Wang Man Wang Min Chen Jing-Ming Yin Ghullam Murtaza Kaleri Rui-Jie Zhang Tie-Niu Zuo Xiong You Qing Yang | 2014 | Journal of Integrative Plant Biology2014，56，4： | 13 |
| 5 | Hepatitis C virus RNA detection in serum and peripheral blood mononuclear cells of patients with hepatitis C显示文摘HepatitisCvirusRNAdetectioninserumandperipheralbloodmononuclearcelsofpatientswithhepatitisCZHOUPing,CAIQing,CHENYouChun,ZHA... | ZHOU Ping, CAI Qing, CHEN You Chun, ZHANG Mu Sen, GUAN Jian and LI Xiao Juan | 1997 | World Journal of Gastroenterology1997，3，2： | 10 |
| 6 | Cytopathological evaluations combined RNA and protein analyses on defined cell regions using single frozen tissue block显示文摘The co-existence of multiple cell components in tissue samples is the main obstacle for precise molecular evaluation on defined cell types. Based on morphological examination, we developed an efficient approach for paralleled RNA and protein isolations from an identical histological region in frozen tissue section.The RNA and protein samples prepared were sufficient for RT-PCR and Western blot analyses, and the results obtained were well coincident each other as well as with the corresponding parameters revealed from immunohistochemical examinations. By this way, the sampling problem caused by cell-cross contamination can be largely avoided, committing the experimental data more specific to a defined cell type. These novel methods thus allow us to use single tissue block for a comprehensive study by integration of conventional cytological evaluations with nucleic acid and protein analyses. | HONG LI, XIAO YAN CHEN, QING You KONG, JIA LIULaboratory of Cell Biology and Molecular Genetics,College of Basic Medical Sciences, Dalian Medical University, Dalian 116027, China | 2002 | Cell Research2002，12，2： | 10 |
| 7 | First dark matter search results from the PandaX-Ⅰ experiment显示文摘We report on the first dark-matter(DM)search results from PandaX-I,a low threshold dual-phase xenon experiment operating at the China JinPing Underground Laboratory.In the 37-kg liquid xenon target with 17.4 live-days of exposure,no DM particle candidate event was found.This result sets a stringent limit for low-mass DM particles and disfavors the interpretation of previously-reported positive experimental results.The minimum upper limit,3.7×10-44cm2,for the spin-independent isoscalar DM-particle-nucleon scattering cross section is obtained at a DM-particle mass of 49 GeV/c2at 90%confidence level. | XIAO MengJiao XIAO Xiang ZHAO Li CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HU Jie HUANG XingTao JI XiangDong JU YongLin LEI SiAo LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing STEPHENSON Scott TAN AnDi TARL Greg WANG HongWei WANG JiMin WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIE PengWei YOU YingHui ZENG XiongHui ZHANG Hua ZHANG Tao ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，11： | 10 |
| 8 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 9 | PandaX: a liquid xenon dark matter experiment at CJPL显示文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented. | CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing SHI YuJie STEPHENSON Scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 9 |
| 10 | Ubiquitylation of p62/sequestosome1 activates its autophagy receptor function and controls selective autophagy upon ubiquitin stress显示文摘在细胞的 ubiquitin (Ub ) 的改变动态平衡,作为 Ub 知道强调,展示并且影响处于多重条件的细胞的回答,然而,内在的机制不完全地被理解。这里,我们报导 autophagy 受体 p62/sequestosome-1 与结合酶, UBE2D2 和 UBE2D3 的 E2 Ub 交往。内长的 p62 在 Ub 动态平衡的 upregulation 期间经历 E2 依赖的 ubiquitylation,一个条件作为 Ub + 应力称为,那对由 bortezomib 的 Ub overexpression,热吃惊或延长 proteasomal 抑制内在,化学疗法的药。p62 的 Ubiquitylation 破坏 p62 的 UBA 领域的 dimerization,解放它的能力认出为选择 autophagy 的 polyubiquitylated 货物。我们进一步证明这机制可能为在 Ub + 压力条件之上的 autophagy 激活是批评的。机制的描述和在察觉到 Ub 应力并且控制选择 autophagy 的 p62 的规章的角色能帮助理解并且调制细胞的回答到许多内长、环境的挑战,潜在地对 autophagy 相关的病为治疗学的策略的发展打开一条新大街。 | Hong Peng Jiao Yang Guangyi Li Qing You Wen Han Tianrang Li Darning Gao Xiaoduo Xie Byung-Hoon Lee Juan Du Jian Hou Tao Zhang Hai Rao Ying Huang Qinrun Li Rong Zeng Lijian Hui Hongyan Wang Qin Xia Xuemin Zhang Yongning He Masaaki Komatsu Ivan Dikic Daniel Finley Ronggui Hu | 2017 | Cell Research2017，27，5： | 9 |
| 11 | Screening of Serum Biomarkers for Distinguishing between Latent and Active Tuberculosis Using Proteome Microarray显示文摘Objective To identify potential serum biomarkers for distinguishing between latent tuberculosis infection(LTBI) and active tuberculosis(TB). Methods A proteome microarray containing 4,262 antigens was used for screening serum biomarkers of 40 serum samples from patients with LTBI and active TB at the systems level. The interaction network and functional classification of differentially expressed antigens were analyzed using STRING 10.0 and the TB database, respectively. Enzyme-linked immunosorbent assays(ELISA) were used to validate candidate antigens further using 279 samples. The diagnostic performances of candidate antigens were evaluated by receiver operating characteristic curve(ROC) analysis. Both antigen combination and logistic regression analysis were used to improve diagnostic ability. Results Microarray results showed that levels of 152 Mycobacterium tuberculosis(Mtb)-antigenspecific IgG were significantly higher in active TB patients than in LTBI patients(P < 0.05), and these differentially expressed antigens showed stronger associations with each other and were involved in various biological processes. Eleven candidate antigens were further validated using ELISA and showed consistent results in microarray analysis. ROC analysis showed that antigens Rv2031 c, Rv1408, and Rv2421 c had higher areas under the curve(AUCs) of 0.8520, 0.8152, and 0.7970, respectively. In addition, both antigen combination and logistic regression analysis improved the diagnostic ability. Conclusion Several antigens have the potential to serve as serum biomarkers for discrimination between LTBI and active TB. | CAO Shu Hui CHEN Yan Qing SUN Yong LIU Yang ZHENG Su Hua ZHANG Zhi Guo LI Chuan You | 2018 | Biomedical and Environmental Sciences2018，31，7： | 8 |
| 12 | Upregulation of Mark3 and Rpgripl mRNA expression by jujuboside A in mouse hippocampus显示文摘瞄准:在 hippocampus.Methods 在 modulating geneexpression 上调查 jujuboside A (JuA ) 的效果:老鼠的自发的活动被监视,并且 thedifferential 显示聚合酶链反应被改编在老鼠 hippocampus.Results 由 JuA 屏蔽表示差别的 genesmodulated:JuA 显著地减少了全部的 activityintensity (P > 0.01 对控制) 在 80 mg/kg,和基因 MAP/microtubuleaffinity-regulating kinase3 (Mark3 ) 和视网膜炎 pigmentosa GTPase 的剂量,管理者交往 proteinl (Rpgripl )是由在鼠标 hippocampus.Conclusion 的 JuA 的 upregulated :JuA 有禁止的 effecton 老鼠的自发的活动,和 JuA 调整了 Mark3 和 Rpgrip1 inthe 老鼠马头鱼尾的怪兽的抄写。 | Cheng WANG Zi-li YOU Qing XIA Tao XIONG Yang XIA De-zhong YAO | 2007 | Acta Pharmacologica Sinica2007，28，3： | 8 |
| 13 | Power electronic transformer with adaptive PLL technique for voltage-disturbance ride through显示文摘The power electronic transformer(PET) has recently emerged as a type of power converter. It features the basic functions of power conversion and isolation as well as additional functions related to power quality control. A novel PET for a distribution grid called a flexible power distribution unit is proposed in this paper, and the energy exchange mechanism between the network and the load is revealed. A 30 kW 600 VAC/220 VAC/110 VDC medium-frequency isolated prototype is developed and demonstrated. This paper also presents key control strategies of the PET for electrical distribution grid applications,especially under grid voltage disturbance conditions.Moreover, stability issues related to the grid-connected three-phase PET are discussed and verified with an impedance-based analysis. The PET prototype is tested, and it passes the voltage-disturbance ride-through function. The experimental results verify the power quality control abilities of the PET. | Jianhua WANG Fangfang LUO Qing DUAN Zhendong JI Binshi GU Jun YOU Wei GU Jianfeng ZHAO | 2018 | Journal of Modern Power Systems and Clean Energy2018，6，5： | 7 |
| 14 | Alteration of microRNA expression in cerebrospinal fluid of unconscious patients after traumatic brain injury and a bioinformatic analysis of related single nucleotide polymorphisms显示文摘 | Wen-Dong You Qi-Lin Tang Lei Wang Jin Lei Jun-Feng Feng Qing Mao Guo-Yi Gao Ji-Yao Jiang | 2016 | Chinese Journal of Traumatology2016，19，1： | 7 |
| 15 | PPC-based Reactive Hot Melt Polyurethane Adhesive(RHMPA)--Efficient Glues for Multiple Types of Substrates显示文摘Tens of billion metric tons of anthropogenic CO_2 discharged from the burning of fossil fuels lead to an enormous environmental and resource burden. It is charming to transform CO_2 to desirable, economical chemicals and materials. Poly(propylene carbonate)(PPC) is an emerging CO_2-based material. Herein, we report the design, synthesis and characterization of the reactive hot melt polyurethane adhesive(RHMPA) based on PPC polyol. The resultant RHMPAs exhibit good adhesion properties to multiple substrates including plastics(PC, PMMA, ABS) and metals(aluminium, steel), which is comparable to or even better than conventional RHMPAs prepared from petro-based polyol. Furthermore, the PPC-based RHMPAs have tunable mechanical properties, and are thermally stable in the typical working range of bonding process(up to 270 °C). The study is expected to expand the applications of PPC and provide a new type of CO_2-based renewable and eco-friendly materials. | Zeng-He Liu Ji-Qing Huang Li-Jie Sun Dong Lei Jing Cao Shuo Chen Wen-Chang Shih Feng-Ling Qing Zheng-Wei You | 2018 | Chinese Journal of Polymer Science2018，36，1： | 7 |
| 16 | 3-year Treatment of Tenofovir Alafenamide vs.Tenofovir Disoproxil Fumarate for Chronic HBV Infection in China显示文摘Background and Aims:Tenofovir alafenamide(TAF)has similar efficacy to tenofovir disoproxil fumarate(TDF)but with improved renal and bone safety in chronic hepatitis B patients studied outside of China.We report 3-year results from two phase 3 studies with TAF in China(Clinicaltrials.gov:NCT02836249 and NCT02836236).Methods:Chinese hepatitis B e antigen(HBeAg)-positive and-negative chronic hepatitis B patients with viremia and elevated alanine aminotransferase were randomized 2:1 to TAF or TDF treatment groups and treated in a double-blind fashion for 144 weeks(3 years).Efficacy responses were assessed by individual study while safety was assessed by a pooled analysis.Results:Of the 334 patients(180 HBeAg-positive and 154 HBeAg-negative)randomized and treated,baseline characteristics were similar between groups.The overall mean age was 38 years and 73%were male.The mean HBV DNA was 6.4 log10 IU/mL.The median alanine aminotransferase was 88 U/L,and 37%had a history of antiviral use.At week 144,the proportion with HBV DNA<29 IU/mL was similar among the two groups,with TAF at 83%vs.TDF at 79%,and TAF at 93%vs.TDF at 92%for the HBeAg-positive and-negative patients,respectively.In each study,higher proportions of TAF than TDF patients showed normalized alanine aminotransferase(via the American Association for the Study of Liver Diseases and the China criteria)and showed loss of HBsAg;meanwhile,the HBeAg seroconversion rates were similar.Treatment was well-tolerated among the TAF patients,who showed a smaller median decline in creatinine clearance(−0.4 vs.−3.2 mL/min;p=0.014)and less percentage change in bone mineral density vs.TDF at hip(−0.95%vs.−1.93%)and spine(+0.35%vs.−1.40%).Conclusions:In chronic hepatitis B patients from China,TAF treatment provided efficacy similar to TDF but with better renal and bone safety at 3 years. | Jinlin Hou Qin Ning Zhongping Duan You Chen Qing Xie Fu-Sheng Wang Lunli Zhang Shanming Wu Hong Tang Jun Li Feng Lin Yongfeng Yang Guozhong Gong John FFlaherty Anuj Gaggar Shuyuan Mo Cong Cheng Gregory Camus Chengwei Chen Yan Huang Jidong Jia Mingxiang Zhang GS-US-320-0110 and GS-US-320-0108 China Investigators | 2021 | Journal of Clinical and Translational Hepatology2021，9，3： | 7 |
| 17 | The Effects of Tetrandrine (TT) and Polyvinylpyridine-N-Oxide (PVNO) on Gene Expression of Type Ⅰand Type ⅢCollagens during Experimental Silicosis显示文摘In the screening tests of drugs for silicosis in our laboratory, we found that TT, a type of alkaloid isolated from Stephania tetrandra, could inhibit the development of experimental silicosis of rats and the synthesis of collagen in rat lung. Chest X-rays of silicotic patients treaied with TT for 1-3 years showed obvious changes. The silicotic nodules became smallel and shadows became clearer. PVNO was proved to have anti-silicotic effect on animal and clinically. This presentation reports the effect of them on collagen mRNA.Dot blot results showed that 1 (Ⅰ) and 1 (Ⅲ) mRNA levels increased significantly at 60 and 120 days after the rats were exposed to silica dust. The mRNA levels went down at 1 and 3 months after treated by TT and PVNO. In situ hybridization observation revealed that the silver grains of Type Ⅰand Type Ⅲ collagen were scattered within the fibroblasts in cellular nodules and in thickened interstitium of silicosis tissue. The amounts of mRNA silver grains decreased in the lung tissue treated by TT and PVNO. It was suggested that TT and PVNO may inhibil the gene expression of collagen during | LIU BING-CI HE YU-XIAN MIAO QING WANG HAI-HUA AND YOU BAO-RONG (Institute of Occupational Medicine, 29 Nan Wei Road,Beijing 100050, China) | 1994 | Biomedical and Environmental Sciences1994，7，3： | 7 |
| 18 | Study of differential polymerase chain reaction of C-erbB-2 oncogene amplification in gastric cancer显示文摘METHODSCerbB2oncogeneamplificationwasexaminedbyusingdiferentialpolymerasechainreaction(dPCR)insurgicalandendoscopicspecimen... | JI Feng, PENG Qing Bi, ZHAN Jing Biao and LI You Ming | 1999 | World Journal of Gastroenterology1999，5，2： | 7 |
| 19 | Thermal shock fatigue behavior of TiC/Al_2O_3 composite ceramics显示文摘纯 hot-pressed 氧化铝和 30 wt.% TiC/Al2O3 composites 的热吃惊疲劳行为被学习。composites 的机械性质上的 TiC 和 Al2O3 开始粒子尺寸的效果被讨论。缩进熄灭测试被进行在疲劳裂缝生长(a) 上评估热疲劳温度差别(T) 的效果和热周期(N) 的数字。机械性质和 TiC/Al2O3 composites 的热疲劳抵抗被 TiC 的增加显著地改进。整体的氧化铝和 TiC/Al2O3 composites 的热吃惊疲劳由于真骑车效果(热疲劳) 。裂缝偏转并且衔接是为 composites 的热吃惊疲劳抵抗的改进的占优势的原因。 | SI Tingzhi LIU Ning ZHANG Qing' an YOU Xianqing | 2008 | Rare Metals2008，27，3： | 6 |
| 20 | In vitro and in vivo activity of D-serine in combination with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus显示文摘As D-amino acids play important roles in the physiological metabolism of bacteria,combination of D-amino acids with antibiotics may provide synergistic antibacterial activity. The aim of the study was to evaluate in vitro and in vivo activity of D-serine alone and in combination withβ-lactams against methicillin-resistant Staphylococcus aureus(MRSA) strains, and to explore the possible sensitization mechanisms. The activity of D-serine, β-lactams alone and in combinations was evaluated both in vitro by standard MICs, time–kill curves and checkerboard assays, and in vivo by murine systemic infection model as well as neutropenic thigh infection model. An in vitro synergistic effect was demonstrated with the combination of D-serine and β-lactams against MRSA standard and clinical strains.Importantly, the combinations enhanced the therapeutic efficacy in the animal models as compared toβ-lactam alone groups. Initial mechanism study suggested possible revision of D-alanine-D-alanine residue to D-alanine-D-serine in peptidoglycan by adding of D-alanine in the medium, which may cause decreased affinity to PBPs during transpeptidation. In conclusion, D-serine had synergistic activity in combination with β-lactams against MRSA strains both in vitro and in vivo. Considering the relatively good safety of D-serine alone or in combination with β-lactams, D-serine is worth following up as new anti-MRSA infection strategies. | Qing Wang Yuemeng Lv Jing Pang Xue Li Xi Lu Xiukun Wang Xinxin Hu Tongying Nie Xinyi Yang Yan Q.Xiong Jiandong Jiang Congran Li Xuefu You | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 6 |
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