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| 1 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 2 | Microstructure Evolution and Tensile Properties of Ti_3Al/Ni-based Superalloy Welded Joint显示文摘In the present work,the dissimilar joining of a Ti_3AI-based alloy to a Ni-based superalloy was attempted by gas tungsten arc(GTA) welding technology.Sound joints were successfully achieved by using a Cu—Ni alloy as filler material.According to X-ray energy dispersive spectroscopy and X-ray diffraction analysis results three transitional layers at the weld/Ti_3AI interface were verified as follows:Ti_2AINb phase dissolved with Cu and Ni;AI(Cu,Ni)_2Ti,(Cu,Ni)_2Ti and(Nb,Ti) solid solution;Cu-rich phase and a complex multi-element phase.The In718/weld interface is characterized by solid solutions of Ni,Cu,Cr,Fe and Nb.The average tensile strength of the as-welded joints at room temperature is 1 63 MPa,and after a post—weld heat treatment it is increased slightly to 177 MPa.The fracture occurred at the surfacial layer of the joined Ti_3AI base alloy,indicating that the Ti_2AINb layer dissolved with Cu and Ni is the weak link of the Ti_3AI/ln71 8 joint. | Bingqing Chen Huaping Xiong Bingbing Sun Siyi Tang Shaoqing Guo Xuejun Zhang | 2014 | Journal of Materials Science & Technology2014，30，7： | 7 |
| 3 | Microstructures and mechanical properties of Ti_3Al/Ni-based superalloy joints arc welded with Ti–Nb and Ti–Ni–Nb filler alloys显示文摘Dissimilar joining of Ti3Al-based alloy to Ni-based superalloy has been carried out using gas tungsten arc(GTA) welding technology with Ti–Nb and Ti–Ni–Nb filler alloys.The joint welded with the Ti–Nb filler alloy contained much less interfacial brittle phases than the one using the Ti–Ni–Nb filler alloy.The average room-temperature tensile strength of the joint welded with Ti–Nb was 202 MPa and the strength value of the one welded with Ti–Ni–Nb was 128 MPa.For both fillers,the weak links of the dissimilar joints were the weld/In718 interfaces.The presence of TiNi,TiNi3 and Ni3Nb intermetallic compounds in the joint welded with Ti–Ni–Nb induced microcracks at the weld/In718 interface and deteriorated the mechanical properties of the joint.And the adoption of the Ti–Nb filler alloy decreased the formation tendency of interfacial brittle phases to some extent and thus enhanced the tensile strength of the joint. | Bingqing Chen Huaping Xiong Bingbing Sun Siyi Tang Borui Du Neng Li | 2014 | Progress in Natural Science:Materials International2014，24，4： | 3 |
| 4 | Enabling End-to-End Communication Between Wireless Sensor Networks and the Internet Based on 6Lo WPAN显示文摘In the paradigms of the Internet of things(Io T), sensor nodes could collect the data through the Wireless sensor network(WSN), and could be managed and accessed by human users through the Internet. There are many challenges remain. Taking advantage of IPv6,IPv6 over Low-power personal area networks(6Lo WPANs)implemented on resource constrained devices makes the connection possible and easier. This paper is contributing on constructing architecture of the end-to-end communication system based on 6Lo WPAN gateway, which enabled convergence of IPv6 network and low-power wireless networking, featuring encapsulating 6Lo WPAN adapter layer in a Network adapter driver(NAD) of personal computer. Using real commercial deployment, the result of application test bed shows that the IPv6 hosts could be interactive with IP-based sensor nodes through 6Lo WPAN gateway with acceptable latency and packet loss. | LUO Bingqing SUN Zhixin | 2015 | Chinese Journal of Electronics2015，24，3： | 2 |
| 5 | Tuning the properties of Ni-based catalyst via La incorporation for efficient hydrogenation of petroleum resin显示文摘The hydrogenation of petroleum resin(PR)is an effective process to prepare high value-added hydrogenated PR(HPR).However,the preparation of non-noble metal-based catalysts with high catalytic activity for PR hydrogenation still remains a challenge.Herein,a La promoted Ni-based catalyst is reported through the thermal reduction of quaternary Ni La Mg Al-layered double hydroxides(Ni La Mg Al-LDHs).The incorporation of La is beneficial to the reduction and stability of Ni particles with reduced particle size,and the increased alkalinity effectively mitigates the breakage of molecular chains of PR.As a result,the La promoted Ni-based catalyst exhibits high catalytic activity and excellent stability for PR hydrogenation.A hydrogenation degree of 95.4%and 96.1%can be achieved for HC_(5)PR and HC_(9) PR with less reduced softening point,respectively.Notably,the hydrogenation degree still maintains at 92.7%even after 100 hours’reaction,much better than that without La incorporation or prepared using conventional impregnation method. | Qunhong Liu Jiangtao Yang Hongwei Zhang Hongming Sun Shuzheng Wu Bingqing Ge Rong Wang Pei Yuan | 2022 | Chinese Journal of Chemical Engineering2022，35，5： | 2 |
| 6 | Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton显示文摘Spinosyns are natural broad-spectrum biological insecticides with a double glycosylated polyketide structure that are produced by aerobic fermentation of the actinomycete,Saccharopolyspora spinosa.However,their large-scale overproduction is hindered by poorly understood bottlenecks in optimizing the original strain,and poor adaptability of the heterologous strain to the production of spinosyn.In this study,we genetically engineered heterologous spinosyn-producer Streptomyces albus J1074 and optimized the fermentation to improve the production of spinosad(spinosyn A and spinosyn D)based on our previous work.We systematically investigated the result of overexpressing polyketide synthase genes(spnA,B,C,D,E)using a constitutive promoter on the spinosad titer in S.albus J1074.The supply of polyketide synthase precursors was then increased to further improve spinosad production.Finally,increasing or replacing the carbon source of the culture medium resulted in a final spinosad titer of~70 mg/L,which is the highest titer of spinosad achieved in heterologous Streptomyces species.This research provides useful strategies for efficient heterologous production of natural products. | Ziheng An Hui Tao Yong Wang Bingqing Xia Yang Zou Shuai Fu Fang Fang Xiao Sun Renqiong Huang Yao Xia Zixin Deng Ran Liu Tiangang Liu | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 1 |
| 7 | Secure P2P topology based on a multidimensional DHT space mapping显示文摘Distributed search and routing algorithms based on the DHT(distributed hash table) protocol have attracted considerable attention in structured P2P(peer-to-peer) research as a result of favorable properties such as distribution,self-organization,and high scalability.Compared with a traditional C/S(client/server) network,the probability of peers initiating malicious behavior increases dramatically because of their self-governing and dynamic characteristics,which also make it harder to satisfy the peers' security required by DHT.In this paper,we propose a new secure DHT protocol based on a multidimensional mapping mechanism.This mechanism maps peers to a multidimensional space by dividing the identifiers into groups.Moreover,a series of secure methods and routing algorithms are provided to achieve secure DHT in smaller spaces.Compared with state-ofthe-art approaches,the theoretical analysis and experimental results show that the multidimensional mapping mechanism can effectively improve the average success rate of a resource search by inhibiting malicious behavior. | SUN ZhiXin LUO BingQing CHEN YaDang BU Kai | 2013 | Science China(Information Sciences)2013，56，5： | 1 |
| 8 | Ultra-thin metal-organic framework nanoribbons显示文摘Structure engineering of metal-organic frameworks(MOFs)at the nanometer scale is attracting increasing interest due to their unique properties and new functions that normally cannot be achieved in bulk MOF crystals.Here,we report the preparation of ultra-thin MOF nanoribbons(NRBs)by using metal-hydroxide nanostructures as the precursors.Importantly,this general method can be used to synthesize various kinds of ultra-thin MOF NRBs,such as MBDC(M=Co,Ni;BDC=1,4-benzenedicarboxylate),NiCoBDC,CoTCPP(TCPP=tetrakis(4-carboxyphenyl)porphyrin)and MIL-53(Al)NRBs.As a proof-of-concept application,the as-prepared ultra-thin Co BDC NRBs have been successfully used as a fluorescent sensing platform for DNA detection,which exhibited excellent sensitivity and selectivity.The present strategy might open an avenue to prepare MOF nanomaterials with new structures and unique properties for various promising applications. | Bingqing Wang Meiting Zhao Liuxiao Li Ying Huang Xiao Zhang Chong Guo Zhicheng Zhang Hongfei Cheng Wenxian Liu Jing Shang Jing Jin Xiaoming Sun Junfeng Liu Hua Zhang | 2020 | National Science Review2020，7，1： | 1 |
| 9 | Electrolysis removal of methyl orange dye from water by electrospun activated carbon fibers modified with carbon nanotubes显示文摘 | Yingying Sun Gang Wang Qiang Dong Bingqing Qian Yulan Meng Jieshan Qiu | 2014 | Chemical Engineering Journal2014，，： | 1 |
| 10 | γ-Tocotrienol-induced apoptosis in human gastric cancer SGC-7901 cells is associated with a suppression in mitogen-activated protein kinase signalling显示文摘 | Wenguang Sun Qi Wang Bingqing Chen | 2008 | British Journal of Nutrition2008，99，： | 1 |
| 11 | Impact of low-molecular-weight organic acids on the availability of phenanthrene and pyrene in soil显示文摘 | Wanting Ling Lili Ren Yanzheng Gao Xuezhu Zhu Bingqing Sun | 2009 | Soil Biology and Biochemistry2009，，10： | 1 |
| 12 | Desorption of phenanthrene and pyrene in soils by root exudates显示文摘 | Yanzheng Gao Lili Ren Wanting Ling Shuaishuai Gong Bingqing Sun Yi Zhang | 2009 | Bioresource Technology2009，，4： | 1 |
| 13 | Impact of low-molecular-weight organic acids on the availability of phenanthrene and pyrene in soil显示文摘 | Wanting Ling Lili Ren Yanzheng Gao Xuezhu Zhu Bingqing Sun | 2009 | Soil Biology and Biochemistry2009，，10： | 1 |
| 14 | Derivation of totipotent-like stem cells with blastocyst-like structure forming potential显示文摘It is challenging to derive totipotent stem cells in vitro that functionally and molecularly resemble cells from totipotent embryos.Here,we report that a chemical cocktail enables the derivation of totipotent-like stem cells,designated as totipotent potential stem(TPS)cells,from 2-cell mouse embryos and extended pluripotent stem cells,and that these TPS cells can be stably maintained long term in vitro.TPS cells shared features with 2-cell mouse embryos in terms of totipotency markers,transcriptome,chromatin accessibility and DNA methylation patterns.In vivo chimera formation assays show that these cells have embryonic and extraembryonic developmental potentials at the single-cell level.Moreover,TPS cells can be induced into blastocyst-like structures resembling preimplantation mouse blastocysts.Mechanistically,inhibition of HDAC1/2 and DOT1L activity and activation of RARγsignaling are important for inducing and maintaining totipotent features of TPS cells.Our study opens up a new path toward fully capturing totipotent stem cells in vitro. | Yaxing Xu Jingru Zhao Yixuan Ren Xuyang Wang Yulin Lyu Bingqing Xie Yiming Sun Xiandun Yuan Haiyin Liu Weifeng Yang Yenan Fu Yu Yu Yinan Liu Rong Mu Cheng Li Jun Xu Hongkui Deng | 2022 | Cell Research2022，32，6： | 1 |
| 15 | Dynamic antimicrobial resistant patterns of Escherichia coli from healthy poultry and swine over 10 years in Chongming Island,Shanghai显示文摘Background:Antimicrobial resistance(AMR)is one of the greatest threats to animal and public health.Here,we conducted a dynamic surveillance of Escherichia coli on Chongming Island in Shanghai during 2009-2021 to identify the characteristics and trends of Chongming’s AMR pandemic.Methods:Rectal(cloaca)swabs from four poultry and nine swine farms(Chongming Island,2009-2021)were collected for E.coli strains acquisition.The micro-broth dilution method was used to test antimicrobial susceptibility of E.coli isolates against 10 antimicrobial classes including 15 antimicrobials.Utilizing generalized linear mixed models(GLMMs)and co-occurrence analyses,we further explored the multiple-drug-resistance(MDR)combinations and dynamic patterns of E.coli over 10 years in two food animals.Results:Total of 863 MDR isolates were found among 945 collected E.coli isolates,337 from poultry and 608 from swine.Both isolates exhibited high resistant rates(>70%)to tetracyclines,phenicols,sulfonamides,penicillins,and aminoglycosides(only in swine).The resistant rates of swine isolates to penicillins,aminoglycosides,tetracyclines,phenicols,and polymyxins were significantly higher than those of poultry isolates,whereas resistance to fluoroquinolones was reversed.Resistance to polymyxins decreased similarly in swine(42.4%in 2009 to 0.0%in 2021)and poultry isolates(from 16.5%to 0.0%).However,resistance to other seven antimicrobial classes(excluding carbapenems and penicillins)declined dramatically in swine isolates,particularly fluoroquinolones(from 80.5%to 14.4%),and tendencies of resistance to the seven classes showed markedly divergent patterns in poultry isolates.Using Poisson GLMMs,the AMR carriage since 2016 was significantly lower than that of 2009(odds ratio<1),indicating a decline in the risk of MDR emergence.Furthermore,despite the highly diverse MDR profiles,co-occurrence analysis identified two prominent MDR clusters of penicillins-phenicols-fluoroquinolones in poultry and aminoglycosides-tetracyclines-sulfonamides-phenicols in swine.Conclusions:Our study uncovered vastly distinct AMR patterns and dynamic tendencies of poultry and swine E.coli isolates from Chongming.Meanwhile,Chongming’s AMR status has ameliorated,as indicated by the decline in antimicrobials prevalence(particularly in swine),lower likelihood of MDR emergence and low carbapenem-,cephalosporin-,and polymyxin resistance.Importantly,this surveillance results are the vital basis for future policy development in Chongming and Shanghai. | Chao Lv Jun Shang Wengang Zhang Bingqing Sun Min Li Chaoyi Guo Nan Zhou Xiaokui Guo Shixin Huang Yongzhang Zhu | 2022 | Infectious Diseases of Poverty2022，11，5： | 0 |
| 16 | 在成纤维细胞中通过一个化学小分子诱导的类XEN状态实现直接重编程显示文摘文章简介直接谱系重编程已经成为了一种具备应用前景的制备目的细胞类型的新方法。在通过化学小分子将小鼠成纤维细胞诱导为多能干细胞形成的过程中。 | Xiang Li Defang Liu Yantao Ma Xiaomin Du Junzhan Jing Lipeng Wang Bingqing Xie Da Sun Shaoqiang Sun Xueqin Jin Xu Zhang Ting Zhao Jingyang Guan Zexuan Yi Weifeng Lai Ping Zheng Zhuo Huang Yanzhong Chang 柴真 Jun Xu 邓宏魁 | 2018 | 科学新闻2018，0，4： | 0 |
| 17 | Research on the Model of a Lightweight Resource Addressing显示文摘This paper discussed the characteristics of addressing from the perspective of Internet addressing mechanism. An Internet of things(IOT) resource addressing iteration model was defined. In the model, a direct addressing mode for active nodes and an indirect addressing mode for passive codes were proposed, which meet the requirement for multiple encoding mode. A unified IOT resource lightweight addressing scheme based on IPv6 has been proposed to implement the two addressing modes. The scheme utilized the virtual domain to solve the problem of the heterogeneous encoding. The paper implemented the addressing process from the Internet host to the sensor node based on IPv6 over low-power wireless personal area networks(6Lo WPAN) protocol. The experiment results show that the scheme is performed to realize communication between wireless sensor networks and IPv6 networks. | LUO Bingqing SUN Zhixin | 2015 | Chinese Journal of Electronics2015，24，4： | 0 |
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