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| 1 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 2 | Genome Sequences Provide Insights into the Reticulate Origin and Unique Traits of Woody Bamboos显示文摘Polyploidization is a major driver of speciation and its importance to plant evolution has been well recognized.Bamboos comprise one diploid herbaceous and three polyploid woody lineages,and are members of the only major subfamily in grasses that diversified in forests,with the woody members having a tree-like lignified culm.In this study,we generated four draft genome assemblies of major bamboo lineages with three different ploidy levels(diploid,tetraploid,and hexaploid).We also constructed a high-density genetic linkage map for a hexaploid species of bamboo,and used a linkage-map-based strategy for genome assembly and identification of subgenomes in polyploids.Further phylogenomic analyses using a large dataset of syntenic genes with expected copies based on ploidy levels revealed that woody bamboos originated subsequent to the divergence of the herbaceous bamboo lineage,and experienced complex reticulate evolution through three independent allopolyploid events involving four extinct diploid ancestors.A shared but distinct subgenome was identified in all polyploid forms,and the progenitor of this subgenome could have been critical in ancient polyploidizations and the origin of woody bamboos.Important genetic clues to the unique flowering behavior and woody trait in bamboos were also found.Taken together,our study provides significant insights into ancient reticulate evolution at the subgenome level in the absence of extant donor species,and offers a potential model scenario for broad-scale study of angiosperm origination by allopolyploidization. | Zhen-Hua Guo Peng-Fei Ma Guo-Qian Yang Jin-Yong Hu Yun-Long Liu En-Hua Xia Mi-Cai Zhong Lei Zhao Gui-Ling Sun Yu-Xing Xu You-Jie Zhao Yi-Chi Zhang Yu-Xiao Zhang Xue-Mei Zhang Meng-Yuan Zhou Ying Guo Cen Guo Jing-Xia Liu Xia-Ying Ye Yun-Mei Chen Yang Yang Bin Han Choun-Sea Lin Ying Lu De-Zhu Li | 2019 | Molecular Plant2019，12，10： | 13 |
| 3 | Hangzhou criteria as downstaging criteria in hepatocellular ca显示文摘Background:The downstaging of hepatocellular carcinoma(HCC)has been confirmed to benefit liver transplantation(LT)patients whose tumors are beyond the transplantation criteria.Milan criteria(MC),a tumor size and number-based assessment,is currently used as the endpoint in these patients.However,many studies believe that tumor biological behavior should be added to the evaluation criteria for downstaging efficacy.Hence,this study aimed to explore the feasibility of Hangzhou criteria(HC),which introduced tumor grading and alpha-fetoprotein in addition to tumor size and number,as an endpoint of downstaging.Methods:We performed a multicenter and retrospective study of 206 patients accepted locoregional therapy(LRT)as downstaging/bridge treatment prior to LT in three centers of China.Results:Recipients were divided into four groups:failed downstaging to the HC(group A,n=46),successful downstaging to the HC(group B,n=30),remained within the HC all the time(group C,n=113),and tumor progressed(group D,n=17).The 3-year HCC recurrence probabilities of groups B and C were not significantly different(10.3%vs.11.6%,P=0.87).The HCC recurrent rate was significantly higher in group A(52.3%)compared with that in group B/C(P<0.05).Seven patients(7/76,9.2%)whose tumor exceeded the the HC were successfully downstaged to the MC,and 39.5%(30/76)to the the HC.In group B,23 patients remained beyond the MC and their survivals were as well as those of patients within the MC.Conclusions:Compared to the MC,HC downstaging criteria can give more HCC patients access to LT and furthermore,the outcome of these patients is the same as those matching MC downstaging criteria.Hangzhou downstaging criteria therefore is applicable in clinical practice. | Qi-Fan Zhan Sun-Bin Ling Yi-Nan Deng Qiao-Nan Shan Qian-Wei Ye Sheng-Jun Xu Guang-Jiang Jiang Di Lu Xu-Yong Wei Li Zhuang Wu Zhang Tian Shen Bei-Ni Cen Hai-Yang Xie Ji-Min Liu Jian Wu Shu-Sen Zheng Yang Yang Xiao Xu | 2020 | Hepatobiliary & Pancreatic Diseases International2020，19，4： | 11 |
| 4 | Bottom water temperature measurements in the South China Sea,eastern Indian Ocean and western Pacific Ocean显示文摘文章报道了一批新的海底底水温度(BWT)数据,其中南海(SCS)158个站位、东印度洋(EIO)30个站位及西太平洋(WPO)37个站位。基于这批新的BWT数据,获得南海和西太平洋海域底水温度与水深经验关系,可为地球物理和物理海洋提供准确、可靠的海底温度边界。这将有助于海底油气资源调查与评估。同时,这批实测数据表明:1)水深超过3500m的海域,其底水温度在南海约为2.47℃,比东印度洋(~1.34℃)和西太平洋(~1.60℃)稍微偏高。这与大洋传送带模式所预测的情况比较吻合。该模式认为:低温高盐的海水,从北大西洋格陵兰岛和冰岛附近海域下沉到深层,然后向南流动,再与南极洲周围海域的低温高盐海水一同向北进入印度洋和太平洋。而南海是一个相对比较封闭的热带边缘海,其内部海水与印度洋和菲律宾海交换有限,导致海水温度整体高于印度洋和太平洋。2)台西南盆地水深在2700~3000m的部分站位,其底水温高达约3.00℃,明显高于其周边同水深海域底水温度(平均值约为2.33℃)。这可能是台西南盆地海底水热活动导致的结果。3)在东印度洋和西太平洋水深超过4800m海域,底水温度随着水压增大稍有升高,其升高率分别为10.6mK·MPa^(-1)和12.0mK·MPa^(-1)。这与理论估算的深层底水绝热压力温度梯度范围较为吻合。这也意味着东印度洋和西太平洋深层底水,主要由绝热自压作用导致其温度随着深度的增大而升高。 | YANG Xiaoqiu SHI Xiaobin ZHAO Junfeng YU Chuanhai GAO Hongfang CHEN Aihua LU Yuanzheng CEN Xianrong LIN Weiren ZENG Xin XU Hehua REN Ziqiang ZHOU Shengqi XU Ziying SUN Jinlong KAMIYA Nana LIN Jian | 2018 | 热带海洋学报2018，37，5： | 8 |
| 5 | Influence of ash composition on the sintering behavior during pressurized combustion and gasification process显示文摘To determine the ash characteristics during fluidized bed combustion and gasification purposes, the investigation of the impacts of chemical composition of Jincheng coal ash on the sintering temperature was conducted. A series of experiments on the sintering behavior at 0.5 MPa was performed using the pressurized pressure-drop technique in the combustion and gasification atmospheres. Meanwhile, the mineral transformations of sintered ash pellets were observed using X-ray diffractometer (XRD) analyzer to better understand the experimental results. In addition, quantitative XRD and field emission scanning electron microscope/energy dispersive X-ray spectrometer (FE-SEM/EDS) analyses of ash samples were used for clarifying the detailed ash melting mechanism. These results show that the addition of Fe2O3 can obviously reduce the sintering temperatures under gasification atmospheres, and only affect a little the sintering temperature under combustion atmosphere. This may be due to the presence of iron-bearing minerals, which will react with other ash compositions to produce low-melting-point eutectics. The FE-SEM/EDS analyses of ash samples with Fe2O3 additive show consistent results with the XRD measurements. The CaO and Na2O can reduce the sintering temperatures under both the combustion and gasification atmospheres. This can be also contributed to the formation of low-melting-point eutectics, decreasing the sintering temperature. Moreover, the fluxing minerals, such as magnetite, anhydrite, muscovite, albite and nepheline, contribute mostly to the reduction of the sintering temperature while the feldspar minerals, such as anorthite, gehlenite and sanidine, can react with other minerals to produce low-melting-point eutectics, and thereby reduce the sintering temperatures. | Ni-jie JING Qin-hui WANG Yu-kun YANG Le-ming CHENG Zhong-yang LUO Ke-fa CEN | 2012 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2012，13，3： | 7 |
| 6 | Effect on platelet aggregation activity: extracts from 31 Traditional Chinese Medicines with the property of activating blood and resolving stasis显示文摘OBJECTIVE: To evaluate the anti-platelet aggregation effects of extracts from 31 Traditional Chinese Medicines(TCM) with the property of activating blood and resolving stasis in terms of TCM theory.METHODS: The 31 TCMs extracts were prepared using water, 90% ethanol and ethyl acetate., and the effects on anti-platelet aggregation were tested on a platelet aggregation analyzer in vitro with adenosine 5'-diphosphate, bovine thrombin and arachi-donic acid(AA) as aggregation inducers, respectively. Aspirin was the positive control.RESULTS: Lots of the tested TCMs had inhibitory effects with concentration-dependent manner on platelet aggregations induced by various agonists.Especially, some of the TCMs such as Chuanxiong(Rhizoma Chuanxiong), Yanhusuo(Rhizoma Corydalis Yanhusuo) and Danshen(Radix Salviae Miltiorrhizae) showed good anti-platelet aggregation effect similar or higher than that in positive control group.CONCLUSION: The study provided scientific references that several TCMs such as Chuanxiong(Rhizoma Chuanxiong), Yanhusuo(Rhizoma Corydalis Yanhusuo) and Danshen(Radix Salviae Miltiorrhizae),possess the property of anti-platelet aggregation. | Chen Cen Wang Fengqin Xiao Wen Xia Zhining Hu Guang Wan Jianbo Yang Fengqing | 2017 | Journal of Traditional Chinese Medicine2017，37，1： | 7 |
| 7 | Plant phylogenomics based on genome-partitioning strategies:Progress and prospects显示文摘The rapid expansion of next-generation sequencing(NGS) has generated a powerful array of approaches to address fundamental questions in biology. Several genome-partitioning strategies to sequence selected subsets of the genome have emerged in the fields of phylogenomics and evolutionary genomics.In this review, we summarize the applications, advantages and limitations of four NGS-based genomepartitioning approaches in plant phylogenomics: genome skimming, transcriptome sequencing(RNAseq), restriction site associated DNA sequencing(RAD-Seq), and targeted capture(Hyb-seq). Of these four genome-partitioning approaches, targeted capture(especially Hyb-seq) shows the greatest promise for plant phylogenetics over the next few years. This review will aid researchers in their selection of appropriate genome-partitioning approaches to address questions of evolutionary scale, where we anticipate continued development and expansion of whole-genome sequencing strategies in the fields of plant phylogenomics and evolutionary biology research. | Xiangqin Yu Dan Yang Cen Guo Lianming Gao | 2018 | Plant Diversity2018，40，4： | 6 |
| 8 | Peripheral Lymphocyte Subsets as a Marker of Parkinson's Disease in a Chinese Population显示文摘In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson's disease(PD) to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer(NK) cells and CD3+, CD3+CD4+, CD3+CD8+, and CD19+ lymphocytes from peripheral blood were determined by immunostaining and flow cytometric analysis and the percentages of these CD+ T cells were calculated. The ratio of regulatory T(Treg)/helper T 17(Th17) lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis.The results showed that the percentage of NK cells was higher in advanced PD patients than in controls(22.92% ±10.08% versus 19.76% ± 10.09%, P = 0.006), while CD3+ T cells are decreased(62.93% ± 9.27% versus65.75% ± 9.13%, P = 0.005). The percentage of CD19+B cells in male patients was lower(P = 0.021) than in female patients, whereas NK cells were increased(P \ 0.0001). The scores on the Unified Parkinson's Disease Rating Scale(UPDRS) and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in earlyonset patients(P = 0.024 and P = 0.007, respectively). The percentage of CD19+ B cells in patients with UPDRS scores[24 was lower than in those with scores \24(10.17% ±4.19% versus 12.22% ± 5.39%, P = 0.009). In addition, the Treg/Th17 ratio in female patients was higher than that in female controls(13.88 ± 6.32 versus 9.94 ± 4.06, P =0.042). These results suggest that the percentages of NK cells,CD3+ T cells, and CD19+ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs. | Luan Cen Chaohao Yang Shuxuan Huang Miaomiao Zhou Xiaolu Tang Kaiping Li Wenyuan Guo Zhuohua Wu Mingshu Mo Yousheng Xiao Xiang Chen Xinling Yang Qinhui Huang Chaojun Chen Shaogang Qu Pingyi Xu | 2017 | Neuroscience Bulletin2017，33，5： | 5 |
| 9 | HNCO hydrolysis performance in urea-water solution thermohydrolysis process with and without catalysts显示文摘The thermolysis of urea-water solution and its product, HNCO hydrolysis is investigated in a dual-reactor system. For the thermal decomposition below about 1073 K, the main products are ammonia (NH3) and isocyanic acid (HNCO) whereas at higher temperatures the oxidation processes take effect and the products include a low concentration of nitric oxide (NO) and nitrous oxide (N2O). The gas HNCO is quite stable and a high yield of HNCO is observed. The ratio of NH3 to HNCO increases from approximately 1.2 to 1.7 with the temperature. The chemical analysis shows that H radical is in favor of HNCO hydrolysis by instigating the reaction HNCO+H·→·NH2+CO and high temperature has positive effect on H radical. The hydrolysis of HNCO over an alumina catalyst made using a sol-gel process (designated as γ-Al2O3) is investigated. The conversion of HNCO is high even at the high space velocities (6×105 h-1) and low temperatures (393–673 K) in the tests with catalysts, which enhances HNCO hydrolysis and raises the ratio of NH3 to HNCO to approximately 100. The pure γ-Al2O3 shows a better catalytic performance than CuO/γ-Al2O3. The addition of CuO not only reduces the surface area but also decreases the Lewis acid sites which are recognized to have a positive effect on the catalytic activity. The apparent activation energy of the hydrolysis reaction amounts to about 25 kJ/mol in 393–473 K while 13 kJ/mol over 473 K. The overall hydrolysis reaction rate on catalysts is mainly determined by external and internal mass-transfer limitations. | Zhen-chao CHEN Wei-juan YANG Jun-hu ZHOU Hong-kun LV Jian-zhong LIU Ke-fa CEN | 2010 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2010，11，11： | 5 |
| 10 | Premixed jet flame characteristics of syngas using OH planar laser induced fluorescence显示文摘Lean premixed flame characteristics of several typical low calorific value (LCV) syngases (basis CO/H2/CH4/CO2/N2),including bituminous coal,wood residue,corn core,and wheat straw gasification syngas,were investigated using OH planar laser induced fluorescence (PLIF) technology.OH radical distributions within the turbulent flame were measured for different turbulence intensities.Flame structures of syngases were analyzed and characterized with respect to burnt and unburnt regions,flame curvature (sharp cusp),local extinction (holes and penetration),OH reaction layer thickness,wrinkling,and other features,with OH-PLIF instantaneous images and statistical analysis.Results show that H2 content,LCV,and turbulence intensity are the most effective factors influencing the OH radical intensity and thickness of OH radical layers.The bituminous coal gasification syngas with relatively higher LCV and H2 content tends to burn out easily.Through changes in thickness of the OH radical layers and signal intensities,the reaction layer can be compressed by intensifying turbulence and thereby the combustion processes of syngas. | YANG Li WANG ZhiHua ZHU YanQun LI ZhongShan ZHOU JunHu HUANG ZhenYu CEN KeFa | 2011 | Chinese Science Bulletin2011，56，26： | 5 |
| 11 | Abrogation of Hn RNP L enhances anti-PD-1 therapy efficacy via diminishing PD-L1 and promoting CD8^(+) T cell-mediated ferroptosis in castration-resistant prostate cancer显示文摘Owing to incurable castration-resistant prostate cancer(CRPC)ultimately developing after treating with androgen deprivation therapy(ADT),it is vital to devise new therapeutic strategies to treat CRPC.Treatments that target programmed cell death protein 1(PD-1)and programmed death ligand-1(PD-L1)have been approved for human cancers with clinical benefit.However,many patients,especially prostate cancer,fail to respond to anti-PD-1/PD-L1 treatment,so it is an urgent need to seek a support strategy for improving the traditional PD-1/PD-L1 targeting immunotherapy.In the present study,analyzing the data from our prostate cancer tissue microarray,we found that PD-L1 expression was positively correlated with the expression of heterogeneous nuclear ribonucleoprotein L(Hn RNP L).Hence,we further investigated the potential role of Hn RNP L on the PD-L1 expression,the sensitivity of cancer cells to T-cell killing and the synergistic effect with anti-PD-1 therapy in CRPC.Indeed,Hn RNP L knockdown effectively decreased PD-L1 expression and recovered the sensitivity of cancer cells to T-cell killing in vitro and in vivo,on the contrary,Hn RNP L overexpression led to the opposite effect in CRPC cells.In addition,consistent with the previous study,we revealed that ferroptosis played a critical role in T-cell-induced cancer cell death,and Hn RNP L promoted the cancer immune escape partly through targeting YY1/PD-L1 axis and inhibiting ferroptosis in CRPC cells.Furthermore,Hn RNP L knockdown enhanced antitumor immunity by recruiting infiltrating CD8^(+)T cells and synergized with anti-PD-1 therapy in CRPC tumors.This study provided biological evidence that Hn RNP L knockdown might be a novel therapeutic agent in PD-L1/PD-1 blockade strategy that enhanced anti-tumor immune response in CRPC. | Xumin Zhou Libin Zou Hangyu Liao Junqi Luo Taowei Yang Jun Wu Wenbin Chen Kaihui Wu Shengren Cen Daojun Lv Fangpeng Shu Yu Yang Chun Li Bingkun Li Xiangming Mao | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 4 |
| 12 | MiR-301a transcriptionally activated by HIF-2αpromotes hypoxiainduced epithelial-mesenchymal transition by targeting TP63 in pancreatic cancer显示文摘BACKGROUND Pancreatic cancer(PC)is one of the deadliest cancers worldwide.PC metastasis involves a complex set of events,including epithelial-mesenchymal transition(EMT),that increase tumor cell invasiveness.Recent evidence has shown that hypoxia is a major EMT regulator in pancreatic cancer cells and facilitates metastasis;however,the mechanisms remain elusive.AIM To investigate the role of miR-301a in hypoxia-induced EMT in PC cells.METHODS Real-time PCR and Western blot analysis were used to detect the expression of miR-301a and EMT markers in PDAC cells cultured in hypoxic and normoxic conditions.Western blot analysis was used to detect the expression of EMT markers in PDAC cells with miR-301a overexpression.Wound healing assay and Transwell assay were used to detect the migration capabilities of PDAC cells with miR-301a overexpression and knockout.Luciferase assay was used to detect the miR-301a promoter and the 3’untranslated region activity of TP63.Orthotopic PC mouse models were used to study the role of miR-301a in metastasis of PDAC cells in vivo.In situ hybridization assay was used to detect the expression of miR-301a in PDAC patient samples(adjacent paratumor and paired tumor tissues).RESULTS Hypoxic environment could directly promote the EMT of PC cells.The expression level of miR-301a was increased in a HIF2αdependent manner in hypoxia-cultured CFPAC-1 and BxPC-3 cells.Overexpression of miR-301a enhanced the hypoxia-induced EMT of PC cells,while knocking out miR-301a result in the suppression of hypoxia-induced EMT.TP63 was a direct target of miR-301a and involved in the metastatic process of PC cells.Furthermore,miR-301a upregulation facilitated PDAC distant metastasis and lymph node metastasis in vivo.Additionally,miR-301a overexpression was indicative of aggressive clinicopathological behaviors and poor prognosis.CONCLUSION The newly identified HIF-2α-miR301a-TP63 signaling pathway may play a crucial role in hypoxia-induced EMT in PDAC cells. | Kun-Dong Zhang Bin Hu Gang Cen Yu-Han Yang Wei-Wei Chen Zeng-Ya Guo Xiao-Feng Wang Qian Zhao Zheng-Jun Qiu | 2020 | World Journal of Gastroenterology2020，26，19： | 4 |
| 13 | Polyethyleneimine-modified calcium carbonate nanoparticles for p53 gene delivery显示文摘In this study,calcium carbonate(CaCO3)nanoparticles with spherical structure were regulated by arginine and successfully synthesized via a facile co-precipitation method.The average particle size of as-prepared CaCO3 was about 900 nm.The properties of nanostructured CaCO3 particles were characterized by scanning electron microscope,Fourier transform infrared spectroscopy,Xray diffraction and size distribution.After modified with polyethyleneimine(PEI),the ability of PEICaCO3 nanoparticles to carry GFP-marked p53 gene(pEGFP-C1-p53)into cancer cells to express P53 protein were studied.Meanwhile,the cytotoxicity,transfection efficiency,cells growth inhibition and the ability to induce apoptosis by expressed P53 protein were conducted to evaluate the performances of PEI-CaCO3 nanoparticles.The results show that prepared PEI-CaCO3 nanoparticles had good biocompatibility and low cytotoxicity in a certain concentration range.PEI-CaCO3 effectively transfected pEGFP-C1 gene into epithelial-like cancer cells.And with the expression of GFP-P53 fusion protein,pEGFP-C1-p53-gene-loaded PEI-CaCO3 particles significantly reduced the proliferation of cancer cells.These findings indicate that our PEI-modified CaCO3 nanoparticles are potential to be successfully used as carriers for gene therapy. | Cen Chen Huafeng Han Wei Yang Xiaoyuan Ren Xiangdong Kong | 2016 | Regenerative Biomaterials2016，3，1： | 4 |
| 14 | Discovery of Wolitu Pb-Zn deposit through geochemical prospecting under loess cover in Inner Mongolia, China显示文摘We report the finding of the Wolitu Pb-Zn deposit in Inner Mongolia, China, through a series of geochemical surveys. The Wolitu area, located in the loess-cover area in the Hure Banner, Tongliao City,Inner Mongolia, and neighboring the Horqin Sandy Land to the north, had no previous history of Pb-Zn mining or record of Pb-Zn mineralization. Our study identified a large Pb-Zn anomaly with potential zones of mineralization by stream sediment survey. Random rock sampling reveals limonitization at sporadic outcrops in the gullies. The high concentrations of Pb in the residual debris provided guidelines to fix the position for exploratory trench. Oxidized concealed orebodies were identified by trenching.Blind orebodies in veins hosted within the structural zone between slates and marbles of the upper Carboniferous Shizuizi Formation and the Permian granite were discovered by drilling. It is computed that the ore reserve may reach up to 540,000 tones with Pb grade of 1.27% and Zn of 1.9%. This case study is an excellent example for identifying potential polymetallic deposits in loess covered terrains using geochemical exploration. | Fan Yang Mu Kong Huazhong Liu Jinsong Yu Shaoping Yang Zhihong Hao Dehui Zhang Kuang Cen | 2017 | Geoscience Frontiers2017，8，5： | 3 |
| 15 | Graphene Array-Based Anti-fouling Solar Vapour Gap Membrane Distillation with High Energy Efficiency显示文摘Photothermal membrane distillation(MD)is a promising technology for desalination and water purification.However,solar-thermal conversion suffers from low energy efficiency(a typical solar-water efficiency of ~50%),while complex modifications are needed to reduce membrane fouling.Here,we demonstrate a new concept of solar vapour gap membrane distillation(SVGMD)synergistically combining self-guided water transport,localized heating,and separation of membrane from feed solution.A free-standing,multifunctional light absorber based on graphene array is custom-designed to locally heat the thin water layer transporting through graphene nanochannels.The as-generated vapour passes through a gap and condenses,while salt/contaminants are rejected before reaching the membrane.The high solar-water efficiency(73.4% at 1 sun),clean water collection ratio(82.3%),excellent anti-fouling performance,and stable permeate flux in continuous operation over 72 h are simultaneously achieved.Meanwhile,SVGMD inherits the advantage of MD in microorganism removal and water collection,enabling the solar-water efficiency 3.5 times higher compared to state-of-the-art solar vapour systems.A scaled system to treat oil/seawater mixtures under natural sunlight is developed with a purified water yield of 92.8 kg m-2 day-1.Our results can be applied for diverse mixed-phase feeds,leading to the next-generation solar-driven MD technology. | Biyao Gong Huachao Yang Shenghao Wu Guoping Xiong Jianhua Yan Kefa Cen Zheng Bo Kostya Ostrikov | 2019 | Nano-Micro Letters2019，11，3： | 3 |
| 16 | Effectiveness and Safety of a Novel Approach for Management of Patients with Potential Difficult Mask Ventilation and Tracheal Intubation: A Multi-center Randomized Trial显示文摘 | Ji-Ming Wang Er-Li Ma Qing-Ping Wu Ming Tian Yan-Yan Sun Jing Lin Liang Peng Qiang Xu Wei Wei Hong Tan Cen Yang Xiao-Qiang Li Yun-Xia Zuo Jin Liu | 2018 | Chinese Medical Journal2018，，6： | 3 |
| 17 | The biomarkers of immune dysregulation and inflammation response in Parkinson disease显示文摘Parkinson’s disease(PD)is referring to the multi-systemicα-synucleinopathy with Lewy bodies deposited in midbrain.In ageing,the environmental and genetic factors work together and overactive major histocompatibility complex pathway to regulate immune reactions in central nerve system which resulting in neural degeneration,especially in dopaminergic neurons.As a series of biomarkers,the human leukocyte antigen genes with its related proteomics play cortical roles on the antigen presentation of major histocompatibility complex molecules to stimulate the differentiation of T lymphocytes and i-proteasome activities under their immune response to the PD-related environmental alteration and genetic variation.Furthermore,dopaminergic drugs change the biological characteristic of T lymphatic cells,affect theα-synuclein presentation pathway,and inhibit T lymphatic cells to release cytotoxicity in PD development.Taking together,the serum inflammatory factors and blood T cells are involved in the immune dysregulation of PD and inspected as the potential clinic biomarkers for PD prediction. | Li Chen Mingshu Mo Guangning Li Luan Cen Lei Wei Yousheng Xiao Xiang Chen Shaomin Li Xinling Yang Shaogang Qu Pingyi Xu | 2016 | Translational Neurodegeneration2016，5，1： | 3 |
| 18 | Association Analysis of Proteasome Subunits and Transporter Associated with Antigen Processing on Chinese Patients with Parkinson's Disease显示文摘 | Ming-Shu Mo Wei Huang Cong-Cong Sun Li-Min Zhang Luan Cen You-Sheng Xiao Guo-Fei Li Xin-Ling Yang Shao-Gang Qu Ping-Yi Xu | 2016 | Chinese Medical Journal2016，，9： | 3 |
| 19 | Atmospheric emission characterization of a novel sludge drying and co-combustion system显示文摘A novel system combining sludge drying and co-combustion with coal was applied in disposing sludge and its atmospheric emission characteristics were tested. The system was composed of a hollow blade paddle dryer, a thermal drying exhaust gas control system,a 75 tons/hr circulating fluidized bed and a flue gas cleaning system. The emissions of NH3, SO2, CH4and some other pollutants released from thermal drying, and pollutants such as NOx, SO2etc. discharged by the incinerator, were all tested. Polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans(PCDD/Fs) in the flue gas from the incinerator were investigated as well. The results indicated that the concentrations of NOx and SO2in the flue gas from the incinerator were 145 and 16 mg/m3, respectively,and the I-TEQ concentration of 2,3,7,8-substitued PCDD/Fs was 0.023 ng I-TEQ/Nm3. All these values were greatly lower than the emission standards of China. In addition, there was no obvious odor in the air around the sludge dryer. The results demonstrated that this drying and co-combustion system is efcient in controlling pollutants and is a feasible way for large-scale treatment of industrial sludge and sewage sludge. | Shengyong Lu Liqin Yang Fa Zhou Fei Wang Jianhua Yan Xiaodong Li Yong Chi Kefa Cen | 2013 | Journal of Environmental Sciences2013，25，10： | 3 |
| 20 | 荚膜红细菌(Rhodobacter capsulatus)hemA基因的高效可溶表达及其酶学性质对比研究(英文)显示文摘研究目的:提供一种酶学性质优良的,可在大肠杆菌体内高活性可溶表达的,具有工业应用前景的5-氨基乙酰丙酸合成酶。创新要点:利用E.coli Rosetta(DE3)表达编码荚膜红细菌5-氨基乙酰丙酸合成酶(RC-ALAS)的hemA基因,实现其在大肠杆菌体内高活性可溶表达。以放射形土壤杆菌和类球红细菌hemA基因表达的5-氨基乙酰丙酸合成酶(AR-ALAS和RS-ALAS)为参照,对分离纯化后的RC-ALAS进行酶学性质对比研究。研究方法:工程菌的构建如图1所示,酶的分离纯化采用镍柱亲和层析和凝胶过滤层析,酶学性质的测定采用对比研究法。重要结论:(1)RC-ALAS酶活性高达198.2 U/mg,比AR-ALAS(151.1 U/mg)和RS-ALAS(116.9 U/mg)分别提高31.2%和69.5%;(2)RC-ALAS对底物琥珀酰辅酶A的专一性常数((kcat/Km)S-CoA)为1.4989,高于AR-ALAS(0.7456)和RS-ALAS(1.1699),具有较高的催化效率;(3)对含有荚膜红细菌hemA基因的工程菌进行补料分批发酵,发酵结束时,ALA的产量高达8.8 g/L(67 mmol/L)。 | Jia-wei LOU Li ZHU Mian-bin WU Li-rong YANG Jian-ping LIN Pei-lin CEN | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，5： | 2 |
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