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| 1 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 2 | Endothelial cells-targeted soluble human Delta-like 4 suppresses both physiological and pathological ocular angiogenesis显示文摘Due to its essential roles in angiogenesis, Notch pathway has emerged as an attractive target for the treatment of pathologic angiogenesis. Although both activation and blockage of Notch signal can impede angiogenesis, activation of Notch signal may be more promising because it was shown that long-term Notch signal blockage resulted in vessel neoplasm. However, an in vivo deliverable Notch ligand with highly efficient Notch-activating capacity has not been developed. Among all the Notch ligands, Delta-like4(Dll4) is specifically involved in angiogenesis. In this study, we generated a novel soluble Notch ligand h D4 R, which consists of the Delta-Serrate-Lag-2 fragment of human Dll4 and an arginine-glycine-aspartate(RGD) motif targeting endothelial cells(ECs). We demonstrated that h D4 R could bind to ECs through its RGD motif and effectively triggered Notch signaling in ECs. Further, we confirmed that h D4 R could suppress angiogenesis in vitro as manifested by network formation assay and sprouting assay. More importantly, h D4 R efficiently repressed neonatal retinal angiogenesis and laser-induced choroidal neovascularization(CNV) as well in vivo. In conclusion, we have developed an in vivo deliverable Notch ligand h D4 R, which suppresses angiogenesis both in vitro and in vivo, thus providing a new approach to tackle excessive angiogenesis relevant disease such as CNV. | YAN XianChun YANG ZiYan CHEN Yan LI Na WANG Li DOU GuoRui LIU Yuan DUAN JuanLi FENG Lei DENG SanMing HAN Hua ZHANG Ping | 2015 | Science China(Life Sciences)2015，58，5： | 5 |
| 3 | Effect of water stress on the structure and chlorophyll content of Sedum tatarinowii Maxim leaves显示文摘 | Na Dong-chen Wang Wen dou Yang Li j ing | 2011 | Medici- hal Plant2011，2，9： | 1 |
| 4 | Effect of cryostructures on the uniaxial compressive strength of frozen clay显示文摘Unconfined uniaxial compressive tests were performed to study the influence of cryostructure on frozen clay's behavior,such as strain-stress,compressive strength,and failure characteristics,at temperatures varying from-10 to-2°C and strain rates varying from 1.0×10-5to 1.0×10-3s-1.Artificial samples were prepared of three types:(1)integral structure,(2)laminar structure,and(3)reticular structure.The impact of temperature,strain rate,and cryostructure on the mechanical properties is discussed.In general,frozen clay with various cryostructures shows strain-softening behavior in the range of testing temperatures and strain rates.For frozen clay of different cryostructures,the ultimate compressive strength increases with increasing strain rate and decreasing temperature.Under the same testing conditions,the ultimate compressive strengths from high to low are in integral samples,laminar samples,and reticular samples.Failure strain of frozen clay generally increases with increasing temperature and does not indicate any correlation with cryostructure or strain rate.The failure mode of integral and reticular samples was shear failure,while laminar samples showed tensile failure. | Jian Wei Wang Hai Peng Li Lei Song Shuai Dou Xin Lei Na | 2017 | Research in Cold and Arid Regions2017，9，3： | 1 |
| 5 | Enhanced oral bioavailability and anti‐tumour effect of paclitaxel by 20(s)‐ginsenoside Rg3 in vivo显示文摘 | Lei‐Qiong Yang Bin Wang Hui Gan Shou‐Ting Fu Xiao‐Xia Zhu Zhuo‐Na Wu Da‐Wei Zhan Ruo‐Lan Gu Gui‐Fang Dou Zhi‐Yun Meng | 2012 | Biopharm Drug Dispos2012，，8： | 1 |
| 6 | Performance calculation and improved model research of direct-drive permanent magnet generator based on FEM显示文摘In some control strategies of the direct-rive permanent magnet generator(DDPMG),the mathematics model is excessively simplified and some complex nonlinear characteristics,such as core saturation and cross-saturation,are generally neglected.To solve this problem,this paper utilizes the frozen element permeability method to compute the armature self-and mutual-inductance,permanent magnet d-and q-axis flux varying with d-axis and q-axis current,then an improved model is presented in which the core saturation and cross-saturation between d-axis and q-axis are considered effectively.Based on this model,the method for computing the performance of the generators is also proposed.Taking a 1.5-MW DDPMG as an example,the time-stepping finite element method(T-S FEM) is adopted to analyze the performance with no-load and loaded conditions,the results show a good agreement with the ones obtained by the improved model.Compared with the simplified model,it is demonstrated that the presented model has the high efficiency and reliability and can provide a good reference for optimization design of DDPMG and other PM motors. | ZHANG Jian LI HeMing LUO YingLi DOU Na CUI XueShen | 2012 | Science China(Technological Sciences)2012，55，8： | 1 |
| 7 | Microengineered platforms for characterizing the contractile function of in vitro cardiac models显示文摘Emerging heart-on-a-chip platforms are promising approaches to establish cardiac cell/tissue models in vitro for research on cardiac physiology,disease modeling and drug cardiotoxicity as well as for therapeutic discovery.Challenges still exist in obtaining the complete capability of in situ sensing to fully evaluate the complex functional properties of cardiac cell/tissue models.Changes to contractile strength(contractility)and beating regularity(rhythm)are particularly important to generate accurate,predictive models.Developing new platforms and technologies to assess the contractile functions of in vitro cardiac models is essential to provide information on cell/tissue physiologies,drug-induced inotropic responses,and the mechanisms of cardiac diseases.In this review,we discuss recent advances in biosensing platforms for the measurement of contractile functions of in vitro cardiac models,including single cardiomyocytes,2D monolayers of cardiomyocytes,and 3D cardiac tissues.The characteristics and performance of current platforms are reviewed in terms of sensing principles,measured parameters,performance,cell sources,cell/tissue model configurations,advantages,and limitations.In addition,we highlight applications of these platforms and relevant discoveries in fundamental investigations,drug testing,and disease modeling.Furthermore,challenges and future outlooks of heart-on-a-chip platforms for in vitro measurement of cardiac functional properties are discussed. | Wenkun Dou Manpreet Malhi Qili Zhao Li Wang Zongjie Huang Junhui Law Na Liu Craig A.Simmons Jason T.Maynes Yu Sun | 2022 | Microsystems & Nanoengineering2022，8，1： | 0 |
| 8 | SYNTHESIS AND STRUCTURE OF Cu(Ⅱ)COMPLEXES BY OXIDATIVE ADDITION OF DIBENZOYL PEROXIDE ON METALLIC COPPER显示文摘Dibenzoyl peroxide undergoes oxidative addition on metallic copperpowder with 2,2′-bipyridine(or imidazole)in a mixed solvent(methanol andtetrahydrofuran),and affords the Cu(Ⅱ)complexes-[Cu(Ce(C6H5COO)2(2,2’-bipy)]H2O(1)and[Cu(C6H5COO)2(C3H4N2)2](2).The structure was solved by direct methodsand Fourier synthesis.C24H20N2O5Cu (1),Mr=479.78,space group P2(1)/c,a=6.986(7),b=18.833(I),c=17.021(3),α=γ=90°,Z=4,V=2218.13,Dc=1.443g/cm\+3,R=0.055Rw=0.062.Complex(2),C20H18N4O4Cu(2),Mr=441.74,space group P2(1)/n,a=8.699(4),b=9.840(6),c=12.399(5),α=γ=90°,β=100.8°,Z=4,V=1010.93,Dc=1.654g/cm\+3,R=0.055,Rw=0.062. | Rui Na YANG Dong Mei WANG Dou Man JIN Henan Institute of Chemistry,Zhengzhou 450003Hua Qin WANG Yao YANG Nanjing University,Nanjing 210008 | 1993 | Chinese Chemical Letters1993，4，2： | 0 |
| 9 | Observation of Arctic surface currents using data from a surface drifting buoy显示文摘During the 10th Chinese Arctic scientific expedition carried out in the summer of 2019,the surface current in the high-latitude areas of the Arctic Ocean was observed using a self-developed surface drifting buoy,which was initially deployed in the Chukchi Sea.The buoy traversed the Chukchi Sea,Chukchi Abyssal Plain,Mendeleev Ridge,Makarov Basin,and Canada Basin over a period of 632 d.After returning to the Mendeleev Ridge,it continued to drift toward the pole.Overall,the track of the buoy reflected the characteristics of the transpolar drift and Chukchi Slope Current,as well as the inertial flow,cross-ridge surface flow,and even the surface disorganized flow for some time intervals.The results showed that:(1)the transpolar drift mainly occurs in the Chukchi Abyssal Plain,Mendeleev Ridge,and western Canada Basin to the east of the ridge where sea ice concentration is high,and the average northward flow velocity in the region between 79.41°N and 86.32°N was 5.1 cm/s;(2)the average surface velocity of the Chukchi Slope Current was 13.5 cm/s,and while this current moves westward along the continental slope,it also extends northwestward across the continental slope and flows to the deep sea;and(3)when sea ice concentration was less than 50%,the inertial flow was more significant(the maximum observed inertial flow was 26 cm/s,and the radius of the inertia circle was 3.6 km). | Hongxia Chen Lina Lin Long Fan Wangxiao Yang Yinke Dou Bingrui Li Yan He Bin Kong Guangyu Zuo Na Liu | 2024 | Acta Oceanologica Sinica2024，43，1： | 0 |
| 10 | A NOVEL SYNTHETIC METHOD AND STRUCTURE OF TETRAKIS(BENZOATE)BIS(TRIPHENYLPHOSPHINE OXIDE)DICOPPER(II).OXIDATIVE ADDITION OF DIBENZOYL ON METALLIC COPPER显示文摘Dibenzoyl peroxide undergoes oxidative addition on metalliccopper with triphenylphosphine in a mixed solvent(acetone,dichloromethaneand trichloromethane),and affords the binuclear copper complex(Cu(C6H5COO)2(OPPh3))2.Crystals are monoclinic,space group A2/a,withcell parameters,a=24.337(3),b=10.566(1),c=21.579(2),β= 93.18(1)°,V=5540(1)3,Z=4,R=0.042,and Rw=0.044 for 5872 observed reflections.Each copper ion is coordinated by four bridging benzoato ligands and onetriphenylphosphine oxide group to form binuclear complexes. | Rui Na YANG Bao Yu XUE and Dou Man JIN Henan Institute of Chemistry,Zhengzhou 450003 KOZO KOZAWA and TOKIKO UCHIDA Science University of Tokyo,Noda,Chiba 278,Japan | 1992 | Chinese Chemical Letters1992，3，9： | 0 |
| 11 | SYNTHESIS AND MAGNETIC PROPERTY OF CARBOXYL BRIDGING DICOPPER(Ⅱ) COMPLEXES显示文摘Two new dicopper(Ⅱ) complexes with two carboxyl bridgings in one molecule[Cu(dm-phen)(HCOO)]2(NO3)2 3H2O(1). [Cu(ds-phen)(CH3COO)]2 (NO3)2 3H2O(2). one dicopper(Ⅱ) complex with one carboxyl bridging and one hydroxo bridging in one molecule [Cu2(OH)(C2H5COO)(ds-phen)2]. (NO3)2 H2O(3) and one cononuclear Cu(Ⅱ) complex [Cu(de-phen)(CF3COO)](NO3).H2O(4)(dm-phen= 2,9-dimethyl-1,10-phenanthrolin)were prepared and characterized. The effective magnetic moment, u elf, was measured and their magnetic properties were discussed. The coordination modes of carboxyl moieties in all the title complexes were proposed. | Zhen Feng XI Rui Na YANG Dou Nan JIN Henan Institute of Chemistry, Zhengzhou 450003 | 1992 | Chinese Chemical Letters1992，3，10： | 0 |
| 12 | SYNTHESIS AND CHARACTERIZATION OF A NOVEL BONDING TRINUCLEAR(Mo/Fe/Mo) COMPLEX (MoCl_4)_2·dppf显示文摘By the reaction of bidentate ligand 1,1’-bis(diphenylphosphino)-ferrocene (dppf) with MoCl4(CH3CN)2, a novel bonding type complex (MoCl4)2· (dppf) was synthesized and characterized by elemental analyses, 31p-NMR, IR and electronic spectra, TG and ICP-AES. Structure of the title complex was proposed. | Zhen Feng XI Rui Na YANG Dou Man JIN~* Henan Institute of Chemistry, Zhengzhou 450003 Jin Chun WU Depart. of Chem. Engin., Zhengzhou Inst. of Tech., Zhengzhou 450002 | 1992 | Chinese Chemical Letters1992，3，10： | 0 |
| 13 | Space advanced technology demonstration satellite显示文摘The Space Advanced Technology demonstration satellite(SATech-01),a mission for low-cost space science and new technology experiments,organized by Chinese Academy of Sciences(CAS),was successfully launched into a Sun-synchronous orbit at an altitude of~500 km on July 27,2022,from the Jiuquan Satellite Launch Centre.Serving as an experimental platform for space science exploration and the demonstration of advanced common technologies in orbit,SATech-01 is equipped with 16 experimental payloads,including the solar upper transition region imager(SUTRI),the lobster eye imager for astronomy(LEIA),the high energy burst searcher(HEBS),and a High Precision Magnetic Field Measurement System based on a CPT Magnetometer(CPT).It also incorporates an imager with freeform optics,an integrated thermal imaging sensor,and a multi-functional integrated imager,etc.This paper provides an overview of SATech-01,including a technical description of the satellite and its scientific payloads,along with their on-orbit performance. | ZHANG XiaoFeng CHEN Wen ZHU XiaoCheng MENG Na HE JunWang BI XingZi ZHANG YongHe SHI Qi LI Fei LIU Rui FENG ZhengGong LIU Liu LI JinSong WU HaiChen XU DongXiao LI TaiJie HUANG JiangJiang LIU Shuo LI TianTong YU XianSheng GAO Yang ZHOU Heng BAN HanYu ZHANG YanLi ZHANG YueTing YANG YingQuan HE Tao DUAN XuLiang CHEN Xin WANG YaMin SUN AnTai ZHANG KuoXiang SUN Ying WANG YaoBin FAN ChengCheng XIONG ShaoLin LI XinQiao WEN XiangYang LING ZhiXing SUN XiaoJin ZHANG Chen BAI XianYong WANG ZhanShan DENG YuanYong TIAN Hui YANG JianFeng XUE HongBo SANG Peng LIU JinGuo ZHENG HuiLong ZHU Xiang HE JianWu LI Hui XU LuXiang XU ShuYan CHEN WenWu LIU ZhenDong WANG ZhaoLi MAO XiangLong GAO Rong LI ZongXuan DING GuoPeng WANG XinYu DOU RunJiang WENG LuBin LUO Hao WANG YaPing LIANG XianFeng FANG ZiRuo | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 14 | Eustachian tube teratoma: A case report显示文摘BACKGROUND Mature teratoma composed of all three basic germ cell layers of the head and neck is a rare disease.Teratomas involving the temporal bone are particularly scarce.CASE SUMMARY A 48-year-old male patient with a history of chronic otitis of the left ear from infancy,for which he had been operated on twice,was referred to our hospital for chronic otitis,cholesteatoma and a middle ear mass.Computed tomography(CT)scan and magnetic resonance imaging(MRI)revealed a eustachian tube teratoma,in which the anterior lower part and posterior upper part were connected by a thin membranaceous tissue.The mass was removed completely under general anesthesia by mastoidectomy.As of last follow-up(2 years post-surgery),the disease had not relapsed.CONCLUSION Pre-operative CT and MRI are necessary for eustachian tube teratoma.Complete surgical resection provided excellent prognosis. | Jin-Ye Li Li-Xin Sun Na Hu Ge-Sheng Song Wei-Qiang Dou Ruo-Zhen Gong Chuan-Ting Li | 2022 | World Journal of Clinical Cases2022，10，1： | 0 |
| 15 | Combining methylated SEPTIN9 and RNF180 plasma markers for diagnosis and early detection of gastric cancer显示文摘Dear Editor,Early diagnosis is critical for successful treatment of gastric adenocarcinoma(GA).However,the sensitivities of tumor markers carcinoembryonic antigen(CEA),cancer antigen 19-9(CA19-9)and CA72-4 for GA detection are approximately 20%[1],and the sensitivities of all markers combined for early gastric cancer detection is still very low[2].DNA methylation plays a major role in tumorigenesis and therefore has obvious potential as a non-invasive biomarker for cancer detection[3].Through genome-wide methylation analysis and histological verification,we previously identified ring finger protein 180(RNF180)as a novel preferentially methylated gene in GA[4,5]. | Yongzhan Nie Xianchun Gao Xiqiang Cai Zhen Wu Qiaoyi Liang Guobing Xu Na Liu Peng Gao Jingyu Deng Hongzhi Xu Zhanlong Shen Changqi Cao Fenrong Chen Nannan Zhang Yongxi Song Mingjun Sun Chengyin Liu Guangpeng Zhou Weili Han Jianhua Dou Huahong Xie Liping Yao Zhiguo Liu Gang Ji Xin Wang Qingchuan Zhao Lei Shang Daiming Fan Xiaoliang Han Jianlin Ren Han Liang Zhenning Wang Jinhai Wang Qi Wu Jun Yu Kaichun Wu the MAGIS Study Group | 2023 | Cancer Communications2023，43，11： | 0 |
| 16 | Mettl13 protects against cardiac contractile dysfunction by negatively regulating C-Cbl-mediated ubiquitination of SERCA2a in ischemic heart failure显示文摘Ischemic heart failure(HF)remains a leading cause of morbidity and mortality.Maintaining homeostasis of cardiac function and preventing cardiac remodeling deterioration are critical to halting HF progression.Methyltransferase-like protein 13(Mettl13)has been shown to regulate protein translation efficiency by acting as a protein lysine methyltransferase,but its role in cardiac pathology remains unexplored.This study aims to characterize the roles and mechanisms of Mettl13 in cardiac contractile function and HF.We found that Mettl13 was downregulated in the failing hearts of mice post-myocardial infarction(MI)and in a cellular model of oxidative stress.Cardiomyocyte-specific overexpression of Mettl13 mediated by AAV9-Mettl13 attenuated cardiac contractile dysfunction and fibrosis in response to MI,while silencing of Mettl13 impaired cardiac function in normal mice.Moreover,Mettl13 overexpression abrogated the reduction in cell shortening,Ca^(2+)transient amplitude and SERCA2a protein levels in the cardiomyocytes of adult mice with MI.Conversely,knockdown of Mettl13 impaired the contractility of cardiomyocytes,and decreased Ca^(2+)transient amplitude and SERCA2a protein expression in vivo and in vitro.Mechanistically,Mettl13 impaired the stability of c-Cbl by inducing lysine methylation of c-Cbl,which in turn inhibited ubiquitination-dependent degradation of SERCA2a.Furthermore,the inhibitory effects of knocking down Mettl13 on SERCA2a protein expression and Ca^(2+)transients were partially rescued by silencing c-Cbl in H_(2)O_(2)-treated cardiomyocytes.In conclusion,our study uncovers a novel mechanism that involves the Mettl13/c-Cbl/SERCA2a axis in regulating cardiac contractile function and remodeling,and identifies Mettl13 as a novel therapeutic target for ischemic HF. | Shuting Yu ZhiYong Sun Xiuzhu Wang Tiantian Ju Changhao Wang Yingqi Liu Zhezhe Qu KuiWu Liu Zhongting Mei Na Li Meixi Lu Fan Wu Min Huang Xiaochen Pang Yingqiong Jia Ying Li Yaozhi Zhang Shunkang Dou Jianhao Jiang Xin Li Baofeng Yang Weijie Du | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 17 | Bio-inspired chiral self-assemblies promoted neuronal differentiation of retinal progenitor cells through activation of metabolic pathway显示文摘Retinal degeneration is a main class of ocular diseases.So far,retinal progenitor cell(RPC)transplantation has been the most potential therapy for it,in which promoting RPCs neuronal differentiation remains an unmet challenge.To address this issue,innovatively designed L/D-phenylalanine based chiral nanofibers(LPG and DPG)are employed and it finds that chirality of fibers can efficiently regulate RPCs differentiation.qPCR,western blot,and immunofluorescence analysis show that right-handed helical DPG nanofibers significantly promote RPCs neuronal differentiation,whereas left-handed LPG nanofibers decrease this effect.These effects are mainly ascribed to the stereoselective interaction between chiral helical nanofibers and retinol-binding protein 4(RBP4,a key protein in the retinoic acid(RA)metabolic pathway).The findings of chirality-dependent neuronal differentiation provide new strategies for treatment of neurodegenerative diseases via optimizing differentiation of transplanted stem cells on chiral nanofibers. | Na Sun Xiaoqiu Dou Zhimin Tang Dandan Zhang Ni Ni Jiajing Wang Huiqin Gao Yahan Ju Xiaochan Dai Changli Zhao Ping Gu Jing Ji Chuanliang Feng | 2021 | Bioactive Materials2021，6，4： | 0 |
| 18 | Mechanochromism of polyurethane based on folding-unfolding of cyano-substituted oligo(p-phenylene)vinylene dimer显示文摘The incorporation of mechanophores,motifs that transform mechanical stimulus into chemical reaction or optical variation,allows creating materials with stressresponsive properties.The most widely used mechanophore generally features a weak bond,but its cleavage is typical an irreversible process.Here,we showed that this problem can be solved by folding–unfolding of a molecular tweezer.We systematically studied the mechanochromic properties of polyurethanes with cyano-substituted oligo(p-phenylene)vinylene(COP)tweezer(DPU).As a control experiment,a class of polyurethanes containing only a single COP moiety(MPU)was also prepared.The DPU showed prominent mechanochromic properties,due to the intramolecular folding–unfolding of COP tweezer under mechanical stimulus.The process was efficient,reversible and optical detectable.However,due to the disability to form either intramolecular folding or intermolecular aggregation,the MPU sample was mechanical inert. | Na Zhang Xiang-Yu Ma Shun Li Yu-Xin Zhang Chen Lv Zheng-Peng Mao Zi-Yi Dou Tai-Sheng Wang | 2023 | Frontiers of Materials Science2023，17，2： | 0 |
| 19 | SYNTHESIS AND CHARACTERIZATION OF ZEROVALENT PALLADIUM COMPLEXES显示文摘A series of nine Pd(0)complexes(L)Pd(L’)[L=bis(diphenylphosphino)methane(dppm),1,2-bis(diphenylphosphino)ethane(dppe),and 1,1’-bis(diphenyl-phosphino)ferrocene(dppf),respectively;L=dibenzylideneacetone(dba),2,3,5,6-tetrachloro-1.4-benzoquinone(q)and 2,3-dichloro-1,4-naphthoquinone(nq)]were synthesized and characterized by means of elemental analyses,IR,UV,1H-NMR and31P-NMR spectra.The redox wave peak potentials of the ferrocenylgroup of complexes with dppf were measured by using CV method.Results showedthat all the diphosphorus ligands behaved as chelating bidentate ligand anddba,q,nq were coordinated to the Pd(0)atom through their C=C moieties,nottheir carbonyl ones.Analytical data also showed that there were interactionsbetween the two different ligands in each complex. | Zhen Feng XI Rui Na YANG Dou Man JIN | 1991 | Chinese Chemical Letters1991，2，4： | 0 |
| 20 | Causative Microorganisms Isolated from Patients with Intra-Abdominal Infections and Their Drug Resistance Profiles:An 11-Year(2011–2021)Single-Center Retrospective Study显示文摘Objective To investigate the distribution and antimicrobial susceptibility of causative microorganisms recovered from patients with intra-abdominal infections(IAIs).Methods A total of 2,926 bacterial and fungal strains were identified in samples collected from 1,679 patients with IAIs at the Peking Union Medical College Hospital between 2011 and 2021.Pathogenic bacteria and fungi were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Antimicrobial susceptibility testing(AST)was performed using the VITEK 2 compact system and the Kirby–Bauer method.AST results were interpreted based on the M100-Ed31 clinical breakpoints of the Clinical and Laboratory Standards Institute.Results Of the 2,926 strains identified,49.2%,40.8%,and 9.5%were gram-negative bacteria,gram-positive bacteria,and fungi,respectively.Escherichia coli was the most prevalent pathogen in intensive care unit(ICU)and non-ICU patients;however,a significant decrease was observed in the isolation of E.coli between 2011 and 2021.Specifically,significant decreases were observed between 2011 and 2021 in the levels of extended-spectrumβ-lactamase(ESBL)-producing E.coli(from 76.9%to 14.3%)and Klebsiella pneumoniae(from 45.8%to 4.8%).Polymicrobial infections,particularly those involving co-infection with gram-positive and gram-negative bacteria,were commonly observed in IAI patients.Moreover,Candida albicans was more commonly isolated from hospital-associated IAI samples,while Staphylococcus epidermidis had a higher ratio in community-associated IAIs.Additionally,AST results revealed that most antimicrobial agents performed better in non-ESBL-producers than in ESBL-producers,while the overall resistance rates(56.9%–76.8%)of Acinetobacter baumanmii were higher against all antimicrobial agents than those of other common gram-negative bacteria.Indeed,Enterococcus faecium,Enterococcus faecalis,S.epidermidis,and S.aureus were consistently found to be susceptible to vancomycin,teicoplanin,and linezolid.Similarly,C.albicans exhibited high susceptibility to all the tested antifungal drugs.Conclusion The distribution and antimicrobial susceptibility of the causative microorganisms from patients with IAls were altered between 2011 and 2021.This finding is valuable for the implementation of evidence-based antimicrobial therapy and provides guidance for the control of hospital infections. | DING Rui MA Rui Rui LIU Ya Li ZHAO Ying GUO Li Na DOU Hong Tao SUN Hong Li LIU Wen Jing ZHANG Li WANG Yao LI Ding Ding YI Qiao Lian XU Ying Chun | 2023 | Biomedical and Environmental Sciences2023，36，8： | 0 |
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