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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Effect of Microstructure Refinement on the Strength and Toughness of Low Alloy Martensitic Steel显示文摘马氏体的微观结构在熄灭,有从 6 亩 m 到 199 亩 m 的优先的奥氏体谷物尺寸的 tempered 17CrNiMo6 钢被光金属组织学( OM )描绘了,与各种各样的优先的奥氏体谷物尺寸扫描电子显微镜学( SEM )和传动电子显微镜学( TEM ) .Theyield 强度和钢的坚韧被测试并且与微观结构特征相关。结果证明在 17CrNiMo6 钢的优先的奥氏体谷物尺寸和马氏体的包尺寸与收益强度跟随一种 Hall-Petch 关系。当优先的奥氏体谷物尺寸从 199 亩 m 被精制到 6 亩 m 时,收益强度由 235 MPa 增加了,当在 77 K 的 Charpy U槽口冲击能多于 8times 改善了时,显示那微观结构精炼在比在增加强度改进抵抗到劈开骨折是更有效的。破裂表面暗示为劈开骨折的联合起来的裂缝路径作为是包被识别。 | Chunfang WANG Maoqiu WANG Jie SHI Weijun HUI Han DONG | 2007 | Journal of Materials Science & Technology2007，23，5： | 50 |
| 3 | Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction显示文摘 | Xiao-E Jia Ke Ma Tao Xu Lei Gao Shuang Wu Cong Fu Wenjuan Zhang Zhizhang Wang Kaiyu Liu Mei Dong Changbin Jing Chunguang Ren Zhiwei Dong Yi Chen Yi Jin Qiuhua Huang Xing Chang Min Deng Li Li Lingfei Luo Jun Zhu Yongjun Dang Hung-Chun Chang Leonard I Zon Yi Zhou Saijuan Chen Weijun Pan | 2015 | Cell Research2015，25，8： | 8 |
| 4 | Biofilm Microenvironment-Responsive Nanotheranostics for Dual-Mode Imaging and Hypoxia-Relief-Enhanced Photodynamic Therapy of Bacterial Infections显示文摘The formation of bacterial biofilms closely associates with infectious diseases.Until now,precise diagnosis and effective treatment of bacterial biofilm infections are still in great need.Herein,a novel multifunctional theranostic nanoplatform based on MnO_(2)nanosheets(MnO_(2)NSs)has been designed to achieve pH-responsive dual-mode imaging and hypoxia-relief-enhanced antimicrobial photodynamic therapy(aPDT)of bacterial biofilm infections.In this study,MnO_(2)NSs were modified with bovine serum albumin(BSA)and polyethylene glycol(PEG)and then loaded with chlorin e_(6)(Ce_(6))as photosensitizer to form MnO_(2)-BSA/PEG-Ce_(6)nanosheets(MBP-Ce_(6)NSs).After being delivered into the bacterial biofilm-infected tissues,the MBP-Ce_(6)NSs could be decomposed in acidic biofilm microenvironment and release Ce_(6)with Mn^(2+),which subsequently activate both fluorescence(FL)and magnetic resonance(MR)signals for effective dual-mode FL/MR imaging of bacterial biofilm infections.Meanwhile,MnO_(2)could catalyze the decomposing of H2O_(2)in biofilm-infected tissues into O_(2)and relieve the hypoxic condition of biofilm,which significantly enhances the efficacy of aPDT.An in vitro study showed that MBP-Ce_(6)NSs could significantly reduce the number of methicillin-resistant Staphylococcus aureus(MRSA)in biofilms after 635nm laser irradiation.Guided by FL/MR imaging,MRSA biofilm-infected mice can be efficiently treated by MBP-Ce_(6)NSs-based aPDT.Overall,MBP-Ce_(6)NSs not only possess biofilm microenvironment-responsive dual-mode FL/MR imaging ability but also have significantly enhanced aPDT efficacy by relieving the hypoxia habitat of biofilm,which provides a promising theranostic nanoplatform for bacterial biofilm infections. | Weijun Xiu Siyu Gan Qirui Wen Qiu Qiu Sulai Dai Heng Dong Qiang Li Lihui Yuwen Lixing Weng Zhaogang Teng Yongbin Mou Lianhui Wang | 2020 | Research2020，，1： | 7 |
| 5 | Effect of Cooling Rate and Vanadium Content on the Microstructure and Hardness of Medium Carbon Forging Steel显示文摘This paper reports the effect of cooling rate on the microstructure and hardness of a kind of medium carbon steel microalloyed with two levels of V content(0.15%and 0.28%)after hot deformation by using single compression tests on a Gleeble-3800 thermal simulator.The results show that cooling rate has a significant effect on the microstructure and hardness of the tested steels.Both the fraction of pearlite and hardness increase with increasing cooling rate,whereas a further increase of the cooling rate above a critical value promotes the formation of acicular ferrite(AF),and thus leads to a decrease of hardness mainly owing to the decrease of pearlite fraction and replacing it by AF and the less effective precipitation strengthening.Increasing V content results in a significant increase of hardness,and this tendency enhances with increasing cooling rate until the formation of AF.Furthermore,increasing V content also significantly enhances the formation of AF structure at a lower cooling rate.The results also suggest that by controlling microstructure,especially the precipitation of fine V(C,N)particles through adjusting postforging cooling,the strengthening and gradient function in one hot-forging part could be obtained. | Weijun Hui Yongjian Zhang Chengwei Shao Silian Chen Xiaoli Zhao Han Dong | 2016 | Journal of Materials Science & Technology2016，32，6： | 7 |
| 6 | Vegfa signaling regulates diverse artery/vein formation in vertebrate vasculatures显示文摘Vascular endothelial growth factor A(Vegfa) signaling regulates vascular development during embryogenesis and organ formation.However,the signaling mechanisms that govern the formation of various arteries/veins in various tissues are incompletely understood.In this study,we utilized transcription activator-like effector nuclease(TALEN) to generate zebrafish vegfaa mutants.vegfaa^(-/-) embryos are embryonic lethal,and display a complete loss of the dorsal aorta(DA) and expansion of the cardinal vein.Activation of Vegfa signaling expands the arterial cell population at the expense of venous cells during vasculogenesis of the axial vessels in the trunk.Vegfa signaling regulates endothelial cell(EC) proliferation after arterial-venous specification.Vegfa deficiency and overexpression inhibit the formation of tip cell filopodia and interfere with the pathfinding of intersegmental vessels(ISVs).In the head vasculature,vegfaa^(-/-) causes loss of a pair of mesencephalic veins(MsVs) and central arteries(CtAs),both of which usually dvelop via sprouting angiogenesis.Our results indicate that Vegfa signaling induces the formation of the DA at the expense of the cardinal vein during the trunk vasculogenesis,and that Vegfa is required for the angiogenic formation of MsVs and CtAs in the brain.These findings suggest that Vegfa signaling governs the formation of diverse arteries/veins by distinct cellular mechanisms in vertebrate vasculatures. | Daqing Jin Diqi Zhu Yabo Fang Yiwei Chen Gaihong Yu Weijun Pan Dong Liu Fen Li Tao P.Zhong | 2017 | Journal of Genetics and Genomics2017，44，10： | 6 |
| 7 | Novel dual fluorescence temperature-sensitive chameleon DNA-templated silver nanocluster pair for intracellular thermometry显示文摘第一次,我们正在报导双荧光的一种新奇类型温度敏感的模板 DNA 的银 nanocluster (AgNC ) 通过杂交在最近配对搁浅单人赛的 AgNC,它包含二。两变色龙 AgNC 对, A-NCP 和 B-NCP,拥有从 15 ~ 45 修改 tioxidant 的 chitosan 聚合物完成相应于温度变化的敏感变化的二座明亮的荧光山峰被开发。修改聚合物能有效地与 perovskite 保护太古的 AgNWs 免受方面反应的伤害,而它不通过绝缘的聚合物的接口堵住搬运人飘移。同样准备的 AgNW/polymer 合成电极在 50 m 的一种扫描尺寸在 10 nm 下面展出了根平均数平方粗糙 ????? | Weijun Zhou Jinbo Zhu Ye Teng Baoji Du Xu Han Shaojun Dong | 2018 | Nano Research2018，11，4： | 5 |
| 8 | Clinical and radiological features of medullary infarction caused by spontaneous vertebral artery dissection显示文摘Background and purpose Medullary infarction(MI)caused by spontaneous vertebral artery dissection(sVAD)is an important type of stroke.It is important to distinguish sVAD from other causes of stroke since the treatment strategies and prognosis were different between them.In this study,we aimed to explore the clinical and radiological features of MI in patients with acute MI caused by sVAD.Methods Patients with acute MI caused by sVAD and non-sVAD in a single tertiary hospital were enrolled from 2010 to 2020.Epidemiologic,clinical and image features were collected and analysed.MI lesions were categorised into three levels rostrocaudally and four arterial groups:anteromedial,anterolateral,lateral and posterior.Results A total of 128 patients with MI were enrolled with 47 cases of sVAD and 81 cases of non-sVAD.Patients with sVAD were younger than those with non-sVAD(med 44 years old vs 58 years old).The sVAD group was less likely to have hypertension(44.68%vs 67.90%;p=0.010)and diabetes(19.15%vs 45.69%;p=0.003),but more likely to have non-sudden onset(27.66%vs 9.87%,p=0.009),minor neck injury(19.15%vs 1.23%;p=0.001)and headache(46.81%vs 7.41%;p=0.000).Vertically,sVAD became more common in caudal medulla than in rostral medulla.Horizontally,the sVAD group was more likely to have lateral MI(91.48%vs 2.96%,p=0.000).In multivariable logistic regression analysis,age,non-sudden onset and headache were independently associated with sVAD with ORs of 0.935(95%CI 0.892 to 0.981,p=0.006),3.507(95%CI 1.060 to 11.599,p=0.040)and 5.426(95%CI 1.673 to 17.599,p=0.005).Conclusion sVAD was not uncommon in patients with MI,especially in patients with lateral MI.Young patients with headache and non-sudden onset should remind clinician the possibility of sVAD. | Chun Yu Zhu Zhu Siying Li Yi Xu Wei Yan Xiaocui Kang Yao Li Qiang Dong Weijun Tang Xiang Han | 2022 | Stroke & Vascular Neurology2022，7，3： | 3 |
| 9 | Integrative square-grid triboelectric nanogenerator as a vibrational energy harvester and impulsive force sensor显示文摘方形格子的摩擦电 nanogenerator (SG-TENG ) 为收获震动的精力并且察觉到冲动的力量被表明。每个广场三维(3D ) 打印了方形的格子充满铝(艾尔) 球。格子结构允许 SG-TENG 在宽广带宽上收获震动的精力并且在不同震动的角度操作。SG-TENG 的最惹人注目的特征是它被放大的能力并且综合。在在平行连接二 SG-TENGs 以后,开电路的电压和电线走火电流显著地在完整的震动的频率范围上被增加。与一个乒乓球球拍是综合的, SG-TENG 能从用球拍击一个砰恶臬球收获震动的精力,在由球拍的直接命中产生一般水准的地方,输出 10.9 | Chuan He Weijun Zhu Guang Qin Gu Tao Jiang Liang Xu Bao Dong Chen Chang Bao Han Dichen Li Zhong Lin Wang | 2018 | Nano Research2018，11，2： | 3 |
| 10 | High-cycle Fatigue Fracture Behavior of Ultrahigh Strength Steels显示文摘<正>The fatigue fracture behavior of four ultrahigh strength steels with different melting processes and therefore different inclusion sizes were studied by using a rotating bar two-point bending fatigue machine in the high-cycle regime up to 10~7 cycles of loading.The fracture surfaces were observed by field emission scanning electron microscopy(FESEM).It was found that the size of inclusion has significant effect on the fatigue behavior. For AISI 4340 steel in which the inclusion size is smaller than 5.5μm,all the fatigue cracks except one did not initiated from inclusion but from specimen surface and conventional S-N curve exists.For 65Si2MnWE and Aermet 100 steels in which the average inclusion sizes are 12.2 and 14.9μm,respectively,fatigue cracks initiated from inclusions at lower stress amplitudes and stepwise S-N curves were observed.The S-N curve displays a continuous decline and fatigue failures originated from large oxide inclusion for 60Si2CrVA steel in which the average inclusion size is 44.4μm.In the case of internal inclusion-induced fractures at cycles beyond about 1×10~6 for 65Si2MnWE and 60Si2CrVA steels,inclusion was always found inside the fish-eye and a granular bright facet(GBF)was observed in the vicinity around the inclusion.The GBF sizes increase with increasing the number of cycles to failure N_f in the long-life regime.The values of stress intensity factor range at crack initiation site for the GBF are almost constant with N_f,and are almost equal to that for the surface inclusion and the internal inclusion at cycles lower than about 1×10~6.Neither fish-eye nor GBF was observed for Aermet 100 steel in the present study. | Weijun HUI Yihong NIE Han DONG Yuqing WENG Chunxu WANG | 2008 | Journal of Materials Science & Technology2008，24，5： | 2 |
| 11 | Switching emissions of two tetraphenylethene derivatives with solvent vapor, mechanical, and thermal stimuli显示文摘Two derivatives of tetraphenylethene (TPE) were synthesized through one step cross McMurry coupling reaction. Both luminogens exhibit aggregation-induced emission (AIE) and crystallization induced emission enhancement (CIEE). The emissions of both luminogens could be switched between blue and green through reversible modulation of morphology with thermal, organic solvent fuming and mechanical stimuli. Thus we provide a possible design strategy for emission switching materials. | SHI JunQing ZHAO WeiJun LI CuiHong LIU ZhengPing BO ZhiShan DONG YuPing DONG YongQiang TANG Ben Zhong | 2013 | Chinese Science Bulletin2013，58，22： | 2 |
| 12 | Azomethine-H as a Highly Selective Fluorescent Probe for Fe^3+ Detection in 100% Aqueous Solution and Its Application in Living Cell Imaging显示文摘 | YU Fang QIN Liyuan SHANG Zhuobin DONG Zhenming WANG Yu JIN Weijun | 2015 | Chemical Research in Chinese Universities2015，31，6： | 2 |
| 13 | Enhanced work-hardening behavior and mechanical properties in ultrafine-grained steels with large-fractioned metastable austenite显示文摘 | Jie Shi Xinjun Sun Maoqiu Wang Weijun Hui Han Dong Wenquan Cao | 2010 | Scripta Materialia2010，，8： | 2 |
| 14 | Charge-controlled permeability of polyelectrolyte microcapsules显示文摘 | TONG Weijun DONG Wenfei GAO Changyou | 2005 | The Journal of Physical Chemistry B2005，，13： | 1 |
| 15 | Steady state electrical–thermal coupling analysis of TSV显示文摘This paper presents a blended analytical electrical–thermal model for steady state thermal analysis of through-silicon-via(TSV) in three-dimensional(3 D) integrated circuits. The proposed analytical model is validated by the commercial FEM tool—COMSOL. The comparison between the results of the proposed analytical formulas and COMSOL shows that the proposed formulas have very high accuracy with a maximum error of 0.1%.Based on the analytical model, the temperature performance of TSV is studied. Design guide lines of TSV are also given as:(1) the radius of the TSV increases, the resistance decreases and the temperature can be increased;(2) the thicker the dielectric layer, the higher the temperature;(3) compared with carbon nanotube, the Cu enlarges the temperature by 34 K, and the W case enlarges the temperature by 41 K. | Jingrui Chai Gang Dong Zheng Mei Weijun Zhu | 2018 | Journal of Semiconductors2018，39，9： | 1 |
| 16 | Novel evidence from Taxus fuana forests for niche-neutral process assembling community显示文摘Background:Understanding the mechanisms underlying community assembly is helpful for conservation and restoration of communities, particularly those that contain rare and endangered species like Taxus fuana, which are endemic to the Western Himalayas. The niche (limiting similarity) vs. neutral (randomness) assembly of the T.fuana forest community in Gyirong County, Tibet, China, was investigated. The net relatedness index (NRI) was calculated using a phylogenetic tree. The phylogenetic characteristics of the community and its relationships with environment were analyzed.Results:The value of the mean NRI at the community level was less than-1.96, indicating that the phylogenetic structure was overdispersed;whereas majority of the NRIs at the tree, shrub, and herb layers were within-1.96 to1.96, indicating random dispersion. Environmental factors accounted for 44.38%, 46.52%, 24.04%, and 14.07%of the variation at the community level, tree, shrub, and herb layer, respectively. The phylogenetic structure at the community level and tree layer were significantly influenced by both topographic and soil factors, while shrub and herb layers tended to be affected by a single environmental factor.Conclusions:Community assembly of the T. fuana forest was simultaneously affected by niche and neutral processes, and their variations were closely related to the environment. Neutral process dominated community assembly in the shrub and herb layers. However, the interaction of limiting similarity and randomness played a dominant role at the community level and tree layer;and contributed to maintenance of biodiversity stability. The synergy of multiple environmental factors had a more obvious influence on community assembly than individual environmental factors, especially at the community level. These findings would help to understand the conservation of rare and endangered tree species, such as T. fuana, in the native community;and highlight the importance of random and non-random processes in assembly and biodiversity maintenance of alpine plant communities. | Tianxiang Li Li Xu Feng Wang Weijun Zhang Junpeng Duan Xiaolu Shen-Tu Yaobin Song Runguo Zang Ming Dong | 2022 | Forest Ecosystems2022，9，3： | 1 |
| 17 | Chemical Joinal on Internet显示文摘 | Du Liming Jin Weijun Dong Chuan Liu Changsong Xu Qingqin | 2000 | 02(3): 0162000，02，3： | 1 |
| 18 | Synthesis of confined Ag nanowires within mesoporous silica via doub- le solvent technique and their catalytic properties 显示文摘 | Huang Xiubing Dong Weijun Wang Ge | 2011 | J Colloid Interface Sci2011，359，1： | 1 |
| 19 | Mechanical behavior of electric resistance welded pipes in pipe-making process显示文摘The changes in the mechanical behavior of electric resistance welded(ERW) pipes before and after the cage roll forming process were investigated through tensile experiments.It is found that the Bauschinger effect does not exist in the pipe product,while the work hardening effect introduced by pipe-making is the direct cause of the mechanical changes.The prestrain introduced during different pipe-making processes are accumulative to the work hardening effect.And the increment of the yield strength for making 244.48×8.94 pipe is approximately 45 MPa,higher than that of hot-rolled plates.It is verified that the strain ε=tD-t is an efficient index representing the work hardening effect from the engineering viewpoint. | TIAN Qingchao DONG Xiaoming SHI Hongde DING Weijun | 2010 | Baosteel Technical Research2010，4，3： | 1 |
| 20 | Grain Refinement in Steels and the Application Trials in China 显示文摘 | Dong Han Sun Xinjun Hui Weijun | 2008 | ISIJ International2008，48，8： | 1 |
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