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| 1 | Effects of insoluble dietary fiber from wheat bran on noodle quality显示文摘Benefiting from its favorable effects in promoting intestinal peristalsis,increasing satiety and reducing postprandial blood sugar,wheat bran insoluble dietary fiber(IDF)could be added to noodles to improve the nutrition value of noodle products.However,the addition of IDF usually results in an adverse effect on the quality characteristics of fiber fortified products,leading to unfavorable texture and consumption drop.It was found that IDF could weaken dough characteristics,thereby causing the quality deterioration of noodles,but the mechanism is unclear as yet.This study aimed to investigate the effects of different amounts of IDF on the texture quality and cooking quality of noodles.The water distribution,rheological properties,pasting properties and cooking properties of the noodles were determined by low-field nuclear magnetic resonance(LF-NMR),dynamic rheometer,and rapid visco-analyzer.The results of the LF-NMR showed that the increased IDF in the noodles resulted in a contraction of relaxation time and an increase in the proportion of loosely bound water.At a high amount of IDF,the water absorption and tensile distance of the noodles significantly decreased and the cooking loss rate increased,with a downward trend in peak viscosity,final viscosity and setback values.Moreover,IDF could improve the storage modulus and loss modulus of sheeted dough and enhance the texture of noodles.Furthermore,the correlations between IDF addition amounts and rheological properties as well as cooking properties were analyzed,and the results confirmed that noodles with the IDF amount of 2%–4%had higher nutrition and quality.This study provides the basis for future development and improvement of IDF-enriched health foods. | Mengxu Lei Jihong Huang Xiaoling Tian Peng Zhou Qi Zhu Limin Li Li Li Sen Ma Xiaoxi Wang | 2021 | Grain & Oil Science and Technology2021，4，1： | 8 |
| 2 | Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis:a randomized,double-blind,placebo-controlled phase 2 trial显示文摘Rheumatoid arthritis(RA)is an aggressive autoimmune arthritis,and current therapies remain unsatisfactory due to low remission rate and substantially adverse effects.Low-dose interleukin-2(Ld-IL2)is potentially a therapeutic approach to further improve the disease.This randomized,double-blind,placebo-controlled trial was undertaken to evaluate the efficacy and safety of Ld-IL2 in patients with active RA.Patients were randomly assigned(1:1)to receive Ld-IL2,defined as a dose of 1 million IU,or placebo in a 12-week trial with a 12-week follow-up.Three cycles of Ld-IL2 or placebo were administered subcutaneously every other day for 2 weeks(a total of 7 doses),followed by a 2-week break.All patients received a stable dose of methotrexate(MTX).The primary outcomes were the proportion of patients achieving the ACR20,DAS28-ESR<2.6,and the change from baseline in CDAI or SDAI at week 24.Secondary endpoints included other clinical responses and safety.The primary outcomes were achieved in the perprotocol population.The improvements from baseline in CDAI and SDAI were significantly greater across time points for the LdIL2+MTX group(n=17)than for the placebo+MTX group(n=23)(P=0.018 and P=0.015,respectively).More patients achieved ACR20 response in the Ld-IL2+MTX group than those in the placebo+MTX group at week 12(70.6%vs 43.5%)and at week 24(76.5%vs 56.5%)(P=0.014).In addition,low Treg and high IL-21 were associated with good responses to Ld-IL2.Ld-IL-2 treatment was well-tolerated in this study.These results suggested that Ld-IL2 was effective and safe in RA.ClinicalTrials.gov number:NCT 02467504. | Xiaoying Zhang Miao Miao Ruijun Zhang Xu Liu Xiaozhen Zhao Miao Shao Tian Liu Yuebo Jin Jiali Chen Huixin Liu Xia Zhang Yun Li Yunshan Zhou Yue Yang Ru Li Haihong Yao Yanying Liu Chun Li Yuhui Li Limin Ren Yin Su Xiaolin Sun Jing He Zhanguo Li | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 7 |
| 3 | Triboelectrification induced UV emission from plasmon discharge显示文摘紫外有的高精力的电磁的放射广泛地在工业生产和科学研究领域被使用。在这个工作,深紫外的轻排出物用 triboelectrification 被获得没有任何额外的电源供应的导致的血浆分泌物。由在聚合物和石英玻璃之间的机械磨擦,摩擦电费用引起一个变化电场,它可以带低压力气体(Ar-Hg ) 的血浆分泌物并且给出 253.7 nm 照耀。连续磨擦引起的紫外光能使三色的黄磷激动并且负担得起明亮白的轻排出物。一个紫外消毒实验证明 98% Escherichia coli 能被紫外照耀在 30 min 打死,它表明有好消毒效果的一台自我动力的消毒仪器被制作。这个工作提供一个新奇图案用低频率的机械磨擦制作一台自我动力的紫外轻射出设备并且认识到联合 triboelectrification 和血浆光,它可以进一步在特殊情形扩展紫外光的申请。 | Chang Bao Han Chi Zhang Jingjing Tian Xiaohui Li Limin Zhang Zhou Li Zhong Lin Wang | 2015 | Nano Research2015，8，1： | 5 |
| 4 | Recent advances in persulfate-based advanced oxidation processes for organic wastewater treatment显示文摘In recent years,with the emergence of new pollutants,the effective treatment of wastewater has become very important.Persulfate-based advanced oxidation processes have been successfully applied to the treatment of wastewater,such as wastewater containing antibiotics,pharmaceuticals and personal care products,dyes,endocrine-disrupting chemicals,chlorinated organic pollutants,and phenolics,for the degradation of refractory organic contaminants.This paper summarizes the production of sulfate radicals,which can be generated by the activation of persulfate via conventional and emerging approaches.The existing problems of persulfate-based advanced oxidation processes were analyzed in detail,including residual sulfates,coexisting factors(coexisting inorganic anions and natural organic matter),and energy consumption.This paper proposes corresponding possible solutions to the problems mentioned above,and this paper could provide a reference for the application of persulfate-based advanced oxidation processes in actual wastewater treatment. | Ke Tian Limin Hu Letian Li Qingzhu Zheng Yanjun Xin Guangshan Zhang | 2022 | Chinese Chemical Letters2022，33，10： | 5 |
| 5 | Effects of preventive acupuncture or moxibustion at the Guanyuan acupoint on expression of interleukin-2 and its receptors in hypothalamus, serum, and uterus of menopausal rats显示文摘BACKGROUND: Previous studies have indicated preventive acupuncture and moxibustion at the Guanyuan point have positive effects on the immune system of menopausal rats. OBJECTIVE: To observe the effects of preventive acupuncture or moxibustion at the Guanyuan point on interleukin-2 (IL-2) and its receptor (IL-2R mRNA) levels in hypothalamus, serum, and uterus of menopausal rats. DESIGN: Randomized control animal experiment. SETTING: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine. MATERIALS: This study was performed at the Laboratory of Beijing University of Chinese Medicine from November 2004 to July 2005. The vaginal smear method was used to determine the estrus cycle of 9.5-month-old Sprague Dawley (SD) female rats. Three continuous estrus cycles were measured, and 160 rats with estrus cycle disorder, weighing (300 ± 20) g, were included in this study. The rats were randomly divided into four groups: preventive acupuncture group (n =48), preventive moxibustion group (n =48), model groups (n =48), and 10-month-old group (n =16). Sixteen 3.5-month-old healthy SD female rats, weighing (240 ± 20) g, were also assigned to the young group. All rats were clean grade and were purchased from the Laboratory Animal Center, Beijing University of Chinese Medicine. The experiment was in accordance with animal ethics standards. METHODS: Acupuncture was applied to the preventive acupuncture group at 10 months of age. The needle was inserted upward and perpendicularly two fen to a point that was two fen below the Guanyuan point. The rats freely moved around during needle retention (20 minutes). Acupuncture was administered twice a week and continued for eight weeks. A moxa cone (small) moxibustion was applied to the preventive moxibustion group at 10 months of age. The rats were caught gently and kept at the same position as the preventive acupuncture group. The moxa cone was ignited and held to the Guanyuan point directly. Only one moxa cone was used each time. After moxibustion, the local skin turned slightly red. Moxibustion was used twice a week and continued for eight weeks. Nothing except gently catching and lying was administered to the model group and the young group. MAIN OUTCOME MEASURES: Radioimmunoassay, in situ hybridization, and similar methods were employed to detect IL-2 in serum and the uterus, as well as IL-2R mRNA expression in the hypothalamus and uterus arcuate nuclei, of the preventive acupuncture group, preventive moxibustion group, model groups (at 12, 14, and 16 months), 10 month old group (at 10 months), and the young group (at 4 months). RESULTS: All 176 rats were included in the final analysis. ① IL-2R mRNA: IL-2R mRNA expression decreased significantly in the uterus of 12-, 14-, and 16-month-old model groups and in the hypothalamus of 14- and 16-month-old model groups (P < 0.05-0.01). Compared to the same month-aged model groups, the expression of IL-2R mRNA increased significantly in the hypothalamus and uterus of 16-month-old preventive acupuncture or moxibustion groups (P < 0.05-0.01).② IL-2: IL-2 in serum of 12- and 14-month-old model groups and in the uterus of 12-, 14-, and 16-month-old model groups decreased significantly compared to the young group (P < 0.05-0.01). IL-2 serum levels increased significantly in the 16-month-old group with preventive moxibustion; in addition, IL-2 expression levels increased significantly in the uterus of the 12-month-old group with preventive moxibustion, as well as the 12- and 16-month-old groups with preventive acupuncture (P < 0.05-0.01). CONCLUSION: As a rat ages and becomes menopausal, IL-2R mRNA expression in the hypothalamus anduterus, as well as IL-2 levels in the serum and uterus, decrease. However, preventive acupuncture and preventive moxibustion can repress the decrease to some extent. This effect is even greater in the late stages of menopause (16-month-old rats). | Xiaohong Li Yuwei He Jieping Xie Lufen Zhang Dengfang Zhou Jinghui Zhai Rui Zhan Jun Zhou Limin Qu Yangchun Tian | 2008 | Neural Regeneration Research2008，3，1： | 5 |
| 6 | Effect of focal adhesion kinase on cytoskeletal arrangement of HepG2 cells induced by hypoxia显示文摘对在 hypoxic HepG2 房间和 HepG2 房间的细胞骨架安排上的 FAK siRNA 的效果的焦点的粘附 kinase (FAK ) 表示由组织缺氧导致了的学习客观。方法 HepG2 房间在 21% O 2 和 1% O 2 是有教养的。词法变化在组织缺氧处理以后被观察。西方的污点被用来测量 FAK 表示。指向 FAK 和向量 pGensil-2 的 mRNA 的 siRNA 表示向量 pshRNA-FAK (作为控制) 被构造,然后 transfected 进 HepG2 房间。西方的污点被用来检测 FAK。有组织缺氧导致的 pshRNA-FAK 的 HepG2 房间 transfected 的细胞骨架安排被 phalloidin 分析。有组织缺氧导致的 pshRNA-FAK 的 HepG2 房间 transfected 的迁移的能力被房间迁居试金分析。结果对待组织缺氧的房间与房间分开的大度显示了更多的伸长的形状。FAK 表示在 hypoxic HepG2 房间增加了。FAK 蛋白质水平被 75.64% ±减少 3.12%(P <
0.01 ) 在 pshRNA-FAK transfection 以后。组织缺氧导致了 HepG2 房间的细胞骨架安排。然而,有组织缺氧导致的 pshRNA-FAK 的 HepG2 房间 transfected 的细胞骨架安排在 1% O 2 被禁止。当房间移植试金出现了,有 pshRNA-FAK 的 HepG 房间 transfected 的移居的数字比控制的显著地低(P <
0.05 ) 。在 hypoxic HCC 的 FAK 的表示可能有一种靠近的关系到 HepG2 房间的细胞骨架安排的结论由组织缺氧导致了。FAK 表示的起来规定可以是细胞骨架安排的机制和组织缺氧导致的 hepatocellular 癌的侵略之一。 | Wei Yan Yu Fu Jiazhi Liao Limin Xia Min Luo Qian Zhu Dean Tian | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，3： | 4 |
| 7 | A recombinant receptor-binding domain in trimeric form generates protective immunity against SARSCoV-2 infection in nonhuman primates显示文摘A safe and effective vaccine is critical to combat the COVID-19 pandemic.Here,we developed a trimeric SARS-CoV-2 receptor-binding domain(RBD)subunit vaccine candidate that simulates the natural structure of the spike(S)trimer glycoprotein.Immunization with the RBD trimer-induced robust humoral and cellular immune responses,and a high level of neutralizing antibodies was maintained for at least 4.5 months.Moreover,the antibodies that were produced in response to the vaccine effectively cross-neutralized the SARS-CoV-2501Y.V2 variant(B.1.351).Of note,when the vaccine-induced antibodies dropped to a sufficiently low level,only one boost quickly activated the anamnestic immune response,conferring full protection against a SARSCoV-2 challenge in rhesus macaques without typical histopathological changes in the lung tissues.These results demonstrated that the SARS-CoV-2 RBD trimer vaccine candidate is highly immunogenic and safe,providing long-lasting,broad,and significant immunity protection in nonhuman primates,thereby offering an optimal vaccination strategy against COVID-19. | Limin Yang Deyu Tian Jian-bao Han Wenhui Fan Yuan Zhang Yunlong Li Wenqiang Sun Yanqiu Wei Xiaodong Tian Dan-dan Yu Xiao-li Feng Gong Cheng Yuhai Bi Yong-tang Zheng Wenjun Liu | 2021 | The Innovation2021，2，3： | 3 |
| 8 | Identification of miRNomes in Human Liver and Hepatocellular Carcinoma Reveals miR-199a/b-3p as Therapeutic Target for Hepatocellular Carcinoma显示文摘 | Jin Hou Li Lin Weiping Zhou Zhengxin Wang Guoshan Ding Qiongzhu Dong Lunxiu Qin Xiaobing Wu Yuanyuan Zheng Yun Yang Wei Tian Qian Zhang Chunmei Wang Qinghua Zhang Shi-Mei Zhuang Limin Zheng Anmin Liang Wenzhao Tao Xuetao Cao | 2011 | Cancer Cell2011，，2： | 3 |
| 9 | The self-assembled nanoparticle-based trimeric RBD mRNA vaccine elicits robust and durable protective immunity against SARS-CoV-2 in mice显示文摘As COVID-19 continues to spread rapidly worldwide and variants continue to emerge,the development and deployment of safe and effective vaccines are urgently needed.Here,we developed an mRNA vaccine based on the trimeric receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)protein fused to ferritin-formed nanoparticles(TF-RBD).Compared to the trimeric form of the RBD mRNA vaccine(T-RBD),TF-RBD delivered intramuscularly elicited robust and durable humoral immunity as well as a Th 1-biased cellular response.After further challenge with live SARS-CoV-2,immunization with a two-shot low-dose regimen of TF-RBD provided adequate protection in hACE2-transduced mice.In addition,the mRNA template of TF-RBD was easily and quickly engineered into a variant vaccine to address SARS-CoV-2 mutations.The TF-RBD multivalent vaccine produced broad-spectrum neutralizing antibodies against Alpha(B.1.1.7)and Beta(B.1.351)variants.This mRNA vaccine based on the encoded self-assembled nanoparticle-based trimer RBD provides a referenee for the design of mRNA vaccines targeting SARS-CoV-2. | Wenqiang Sun Lihong He He Zhang Xiaodong Tian Zhihua Bai Lei Sun Limin Yang Xiaojuan Jia Yuhai Bi Tingrong Luo Gong Cheng Wenhui Fan Wenjun Liu Jing Li | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 3 |
| 10 | Single-atom Sn-Zn pairs in CuO catalyst promote dimethyldichlorosilane synthesis显示文摘Single-atom catalysts are of great interest because they can maximize the atom-utilization efficiency and generate unique catalytic properties;however,much attention has been paid to single-site active components,rarely to catalyst promoters.Promoters can significantly affect the activity and selectivity of a catalyst,even at their low concentrations in catalysts.In this work,we designed and synthesized CuO catalysts with atomically dispersed co-promoters of Sn and Zn.When used as the catalyst in the Rochow reaction for the synthesis of dimethyldichlorosilane,this catalyst exhibited much-enhanced activity,selectivity and stability compared with the conventional CuO catalysts with promoters in the form of nanoparticles.Density functional theory calculations demonstrate that single-atomic Sn substitution in the CuO surface can enrich surface Cu vacancies and promote dispersion of Zn to its atomic levels.Sn and Zn single sites as the co-promoters cooperatively generate electronic interaction with the CuO support,which further facilitates the adsorption of the reactant molecules on the surface,thereby leading to the superior catalytic performance. | Qi Shi Yongjun Ji Wenxin Chen Yongxia Zhu Jing Li Hezhi Liu Zhi Li Shubo Tian Ligen Wang Ziyi Zhong Limin Wang Jianmin Ma Yadong Li Fabing Su | 2020 | National Science Review2020，7，3： | 2 |
| 11 | A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification显示文摘Background:Porcine reproductive and respiratory syndrome virus(PRRSV),and particularly its highly pathogenic genotype(HP-PRRSV),have caused massive economic losses to the global swine industry.Results:To rapidly identify HP-PRRSV,we developed a direct real-time reverse transcription polymerase chain reaction method(dRT-PCR) that could detect the virus from serum specimen without the need of RNA purification.Our dRT-PCR assay can be completed in 1.5 h from when a sample is received to obtaining a result.Additionally,the sensitivity of dRT-PCR matched that of conventional reverse transcription PCR(cRT-PCR) that used purified RNA.The lowest detection limit of HP-PRRSV was 6.3 TCID_(50) using dRT-PCR.We applied dRT-PCR assay to 144 field samples and the results showed strong consistency with those obtained by cRT-PCR.Moreover,the dRT-PCR method was able to tolerate 5-20%(v/v) serum.Conclusions:Our dRT-PCR assay allows for easier,faster,more cost-effective and higher throughput detection of HP-PRRSV compared with cRT-PCR methods.To the best of our knowledge,this is the first report to describe a real-time RT-PCR assay capable of detecting PRRSV in crude serum samples without the requirement for purifying RNA.We believe our approach has a great potential for application to other RNA viruses. | Kang Kang Keli Yang Jiasheng Zhong Yongxiang Tian Limin Zhang Jianxin Zhai Li Zhang Changxu Song Christine Yuan Gou Jun Luo Deming Gou | 2015 | Journal of Animal Science and Biotechnology2015，6，1： | 2 |
| 12 | Upregulated FoxM1 expression induced by hepatitis B virus X protein promotes tumor metastasis and indicates poor prognosis in hepatitis B virus-related hepatocellular carcinoma显示文摘 | Limin Xia Wenjie Huang Dean Tian Hongwu Zhu Yongguo Zhang Hao Hu Daiming Fan Yongzhan Nie Kaichun Wu | 2012 | Journal of Hepatology2012，，3： | 1 |
| 13 | A Yeast BiFC-seq Method for Genome-wide Interactome Mapping显示文摘Genome-wide physical protein±protein interaction(PPI)mapping remains a major challenge for current technologies.Here,we reported a high-efficiency BiFC-seq method,yeastenhanced green fluorescent protein-based bimolecular fluorescence complementation(y EGFPBiFC)coupled with next-generation DNA sequencing,for interactome mapping.We first applied y EGFP-BiFC method to systematically investigate an intraviral network of the Ebola virus.Two-thirds(9/14)of known interactions of EBOV were recaptured,and five novel interactions were discovered.Next,we used the BiFC-seq method to map the interactome of the tumor protein p53.We identified 97 interactors of p53,more than three-quarters of which were novel.Furthermore,in a more complex background,we screened potential interactors by pooling two BiFC libraries together and revealed a network of 229 interactions among 205 proteins.These results show that BiFC-seq is a highly sensitive,rapid,and economical method for genome-wide interactome mapping. | Limin Shang Yuehui Zhang Yuchen Liu Chaozhi Jin Yanzhi Yuan Chunyan Tian Ming Ni Xiaochen Bo Li Zhang Dong Li Fuchu He Jian Wang | 2022 | Genomics, Proteomics & Bioinformatics2022，20，4： | 1 |
| 14 | Identification of miRNomes in Human Liver and Hepatocellular Carcinoma Reveals miR-199a/b-3p as Therapeutic Target for Hepatocellular Carcinoma显示文摘 | Jin Hou Li Lin Weiping Zhou Zhengxin Wang Guoshan Ding Qiongzhu Dong Lunxiu Qin Xiaobing Wu Yuanyuan Zheng Yun Yang Wei Tian Qian Zhang Chunmei Wang Qinghua Zhang Shi-Mei Zhuang Limin Zheng Anmin Liang Wenzhao Tao Xuetao Cao | 2011 | Cancer Cell2011，，2： | 1 |
| 15 | Glucose-regulated protein 78 may play a crucial role in promoting the pulmonary microvascular remodeling in a rat model of hepatopulmonary syndrome显示文摘 | Huiying Zhang Minli Lv Zhongfu Zhao Jiantao Jia Lili Zhang Peng Xiao Limin Wang Chen Li Jingquan Ji Xiaoxia Tian Xujiong Li Yimin Fan Lina Lai Yan Liu Baohong Li Cuiying Zhang Mingshe Liu Jianhong Guo Dewu Han Cheng Ji | 2014 | Gene2014，，1： | 1 |
| 16 | Sources of polycyclic aromatic hydrocarbons to sediments of the Bohai and Yellow Seas in East Asia显示文摘 | Lin Tian Hu Limin Guo Zhigang | 2011 | Journal of Geophysical Research: Atmospheres (1984 2012)2011，116，23： | 1 |
| 17 | Study on electrochemical oxidation of 4-chlorophenol on a vitreous carbon electrode using cyclic voltammetry显示文摘 | Xiaoyue Duan Lifeng Tian Wei Liu Limin Chang | 2013 | Electrochimica Acta2013，，： | 1 |
| 18 | Quaternary ammonium salt Gemini surfactants containing perfluoroalkyl tails catalyzed one-pot Mannich reactions in aqueous media显示文摘 | Shen Wei Wang Limin Tian He | 2008 | Journal of Fluorine Chemistry2008，129，： | 1 |
| 19 | Synthesis of SiC precursors by a two - step sol - gel process and their conversion to SiC powders 显示文摘 | Jinwang Li Jiemo Tian Limin Dong | 2000 | Journal of the European Ceramic Society2000，77，： | 1 |
| 20 | Forimation of dicalcium phosphate on magnesium alloy by micro-arc oxidation coupled with hydrothermal treatment 显示文摘 | Limin Chang Lifeng Tian Wei Liu | 2013 | Corrosion Science2013，72，： | 1 |
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