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| 1 | shRNA targeting HDGF suppressed cell growth and invasion of squamous cell lung cancer显示文摘导出 Hepatoma 的生长因素(HDGF ) ,有 mitogenic 的原子蛋白质和 angiogenic 活动,被报导了主要涉及 tumorigenesis 和非小的房间肺癌症的前进。在这研究, HDGF 表示被 specific-shRNA 与在肺指向 HDGF 的 lentivirus 表示向量击倒有鳞的房间癌 520 房间。shRNA 击倒的 HDGF 在 vitro 并且在由 MTT,板克隆和移植肿瘤模型试金显示了的 vivo 显著地压制了房间增长。另外, HDGF 的组合式的表达式也禁止了出现在 transwell 和 Boyden 实验的房间移植和侵略。因为参予有鳞的房间肺癌症的致病的 oncogene,和 HDGF 可以是为非小的房间肺癌症的一个关键治疗学的目标,我们断定 HDGF 行动。 | Jie Meng Wei Xie Liming Cao Chengping Hu Zhiyuan Zhen | 2010 | Acta Biochimica et Biophysica Sinica2010,42,1: | 19 |
| 2 | Correlation between the coverage percentage of prosthesis and postoperative hidden blood loss in primary total knee arthroplasty显示文摘 | Gao Fuqiang Guo Wanshou Sun Wei Li Zirong Wang Weiguo Wang Bailiang Cheng Liming Kush Nepali | 2014 | Chinese Medical Journal2014,,12: | 18 |
| 3 | Gas desorption characteristics of the high-rank intact coal and fractured coal显示文摘The objective of this work is to study the gas desorption characteristics of the high-rank intact coal and fractured coal.The gas adsorption,mercury porosimetry and gas desorption experiments were carried out in this study.Then,the theories of thermodynamics,diffusion mechanism and desorption kinetics were used to estimate the gas desorption characteristics.The results of gas adsorption experiments show that the initial isosteric adsorption heat of the intact coal is greater than that of the fractured coal,indicating that the gas molecules desorb more easily from fractured coal than intact coal.Using the mercury porosimetry,we find that the diffusion channels of fractured coal are more developed than those of intact coal.The difficult diffusion form dominates in the intact coal during the gas diffusing,while the easy diffusion form dominates in the fractured coal.The results of gas desorption experiments show that the initial gas desorption volume and velocity of the fractured coal are both greater than those of the intact coal.Using the Fick diffusion law,the study calculates the gas diffusion coefficients of the intact coal and fractured coal.The diffusion coefficients of the fractured coal are 2 times and 10 times greater than those of the intact coal at the time of 0-120 and 0-10 min,respectively. | Lu Shouqing Cheng Yuanping Qin Liming Li Wei Zhou Hongxing Guo Haijun | 2015 | International Journal of Mining Science and Technology2015,25,5: | 15 |
| 4 | Progress of the key materials for organic solar cells显示文摘Organic solar cells have attracted academic and industrial interests due to the advantages like lightweight,flexibility and roll-to-roll fabrication.Nowadays,18%power conversion efficiency has been achieved in the state-of-the-art organic solar cells.The recent rapid progress in organic solar cells relies on the continuously emerging new materials and device fabrication technologies,and the deep understanding on film morphology,molecular packing and device physics.Donor and acceptor materials are the key materials for organic solar cells since they determine the device performance.The past 25 years have witnessed an odyssey in developing high-performance donors and acceptors.In this review,we focus on those star materials and milestone work,and introduce the molecular structure evolution of key materials.These key materials include homopolymer donors,D-A copolymer donors,A-D-A small molecular donors,fullerene acceptors and nonfullerene acceptors.At last,we outlook the challenges and very important directions in key materials development. | Yang Tong Zuo Xiao Xiaoyan Du Chuantian Zuo Yuelong Li Menglan Lv Yongbo Yuan Chenyi Yi Feng Hao Yong Hua Ting Lei Qianqian Lin Kuan Sun Dewei Zhao Chunhui Duan Xiangfeng Shao Wei Li Hin-Lap Yip Zhengguo Xiaol Bin Zhang Qingzhen Bian Yuanhang Cheng Shengjian Liu Ming Cheng Zhiwen Jin Shangfeng Yang Liming Ding | 2020 | Science China Chemistry2020,63,6: | 15 |
| 5 | Antioxidant and immunomodulatory activities of a polysaccharide from Flammulina velutipes显示文摘OBJECTIVE: To evaluate the antioxidant and immunomodulatory activities of a unique polysaccharide from the medicinal fungus Flammulina velutipes in vitro.METHODS: Using water extraction and alcohol precipitation, crude polysaccharides were obtained. After purification by DEAE-cellulose 52 ion exchange chromatography and Sephacryl S-300 HR gel filtration chromatography, High performance liquid chromatography equipped with evaporative light-scattering detector, Infrared radiation and Nuclear magnetic resonance were used to evaluate the structure of the polysaccharide. Its immunomodulatory activity was measured by examining the production of nitric oxide(NO) and cytokine secretion, and via lymphocyte proliferation experiments. Its effects on the scavenging activities of hydroxyl radical, superoxide anion and reducing power were also measured.RESULTS: A water-soluble polysaccharide, Flammulina velutipes polysaccharide I-A(FVP I-A), was obtained with a molecular mass of 8.14×104Da determined by high performance gel permeation chromatography. An in vitro antioxidant assay indicated that FVP I-A could scavenge hydroxyl radical, superoxide anion and possessed reducing power and could largely promote NO production and augment the interleukin-1β, interleukin-6, and tumor necrosis factor-α secretion by RAW264.7 macrophages(P<0.05). Moreover, FVP I-A could promote lymphocyte proliferation(P<0.05), and synergistically enhance the augmentation of the proliferation of mouse lymphocytes by concanavalin A and lipopolysaccharides(P<0.01, P<0.05).CONCLUSION: The FVP I-A obtained from Flammulina velutipes possessed antioxidant activity and could enhance non-specific and specific immune responses in vitro. | Ming Wu Xia Luo Xiaoyan Xu Wei Wei Mengyao Yu Nan Jiang Liming Ye Zhirong Yang Xiaofan Fei | 2014 | Journal of Traditional Chinese Medicine2014,34,6: | 14 |
| 6 | Low-temperature processed inorganic hole transport layer for efficient and stable mixed Pb-Sn low-bandgap perovskite solar cells显示文摘Metal halide perovskite solar cells(PSCs)have attracted tremendous attention as an emerging photovoltaic technology due to their high efficiency,low cost and ease of fabrication from earth-abundant materials[1,2].The power conversion efficiencies(PCEs)have been rapidly boosted from 3.8%in the pioneer’s work to a certified 24.2%nowadays in just ten years[3].The PCE breakthroughs in PSCs have mostly adopted full lead-based perovskites(APbX3)with bandgaps of 1.5–1.6 eV. | Qiaolei Han Ying Wei Renxing Lin Zhimin Fang Ke Xiao Xin Luo Shuai Gu Jia Zhu Liming Ding Hairen Tan | 2019 | Science Bulletin2019,64,19: | 13 |
| 7 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018,61,3: | 12 |
| 8 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019,62,2: | 11 |
| 9 | Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1显示文摘 | Liming Yan Yuanyuan Ma Dan Liu Xiaochao Wei Yuna Sun Xiaoyue Chen Huadong Zhao Jingwen Zhou Zhiyong Wang Wenqing Shui Zhiyong Lou | 2012 | Cell Research2012,22,8: | 10 |
| 10 | Relationship between endothelial cell protein C receptor gene 6936A/G polymorphisms and deep venous thrombosis显示文摘背景深静脉的血栓(DVT ) 能导致肺的栓塞,从进肺的手足由于血块(血栓) 的赶出和运动的致命的复杂并发症。与 DVT 开发有关的基因风险因素在凝结蛋白质包括变化,特别 endothelial 蛋白质 C 受体(EPCR ) , anticoaguiation 蛋白质 C (PC ) 小径的一个部件。现在的学习的目的是在 EPCR 基因和这研究包含了的 DVT.Methods 的出现分析在 6936A/G 多型性之间的关系有 DVT 和 71 匹配年龄、匹配性的健康控制的 65 个病人。外部血样品从所有题目被收集。可溶的 EPCR (sEPCR ) 的血浆层次被连接酶的 immunosorbent 试金测量。 Genomic DNA 被提取, EPCR 基因产品被一个标准 PCR reaction.Gene 产品放大乐队们被定序在控制组,识别 EPCR 基因 polymorphisms.Results 在有 6936AG 遗传型的题目的 sEPCR 的水平在有 6936AA 遗传型的题目比那显著地高(( 0.97ic ( OR:1.270 , P=0.044 )并且非糖尿病(或:1.369, P=0.011 ) 在为传统的风险因素调整以后的病人。在 CC 遗传型和在糖尿病患者(P=0.019 ) ,然而并非在非糖尿病患者(P=0.126 ) 的 diseased 容器的数字之间有一个协会。在糖尿病的病人之中, CC 遗传型搬运人有早发作的 CAD 的增加的风险(或: 。2.367, P=0.008 ) 并且更大的累积动脉粥样硬化患者负担与 non-CC 遗传型搬运人相比(Gensini 分数: 31.80 ????????挠潨敬瑳牥汯吮敨????????????????????????????映獡楴杮?????倠??‵??湯汣獵潩?????????????????????????僚?瀠汯浹牯桰獩?潈敷敶??????????????????????? | CHEN Xu-dong TIAN Lu LIMing JIN Wei ZHANG Hong-kun ZHENG Cheng-fei | 2011 | Chinese Medical Journal2011,,1: | 10 |
| 11 | Identification of botanical origin of Chinese unifloral honeys by free amino acid profiles and chemometric methods显示文摘The amino acid contents of five floral sources Chinese honeys(jujube, rape, chaste, acacia, and lungan) were measured using reversed phase high-performance liquid chromatography(RP-HPLC). The results showed that proline was the main amino acid in most of the analyzed samples. Phenylalanine presents at the highest content in chaste honey samples, and the total amino acid contents of chaste honeys were also significantly higher than those of other honey samples. Based on the amino acid contents, honey samples were classified using chemometric methods(cluster analysis(CA), principal component analysis(PCA), and discriminant analysis(DA)). According to the CA results, chaste honeys could be separated from other honeys, while the remaining samples were correctly grouped together when the chaste honey data were excluded. By using DA, the overall correct classification rate reached 100%. The results revealed that amino acid contents could potentially be used as indicators to identify the botanical origin of unifloral honeys. | Zheng Sun Lingling Zhao Ni Cheng Xiaofeng Xue Liming Wu Jianbin Zheng Wei Cao | 2017 | Journal of Pharmaceutical Analysis2017,7,5: | 9 |
| 12 | Endovascular treatment of blood blister-like aneurysms in the internal carotid artery using a Willis covered stent显示文摘Background Despite the current availability of flow diverter devices(FDD), problems remains regarding optimal endovascular treatment(EVT) for blood blister-like aneurysms(BBAs) of the internal carotid artery(ICA). Objective To evaluate the safety and efficacy of EVT of BBAs in the ICA with a Willis covered stent. Methods 20 consecutive patients(5 men and 15 women) with ruptured BBAs underwent EVT using a Willis covered stent in two institutions from March 2013 to March 2018. Clinical observations, angiographic characteristics, and procedural and follow-up outcomes were retrospectively evaluated. Results 20 consecutive patients(5 men and 15 women) with ruptured BBAs underwent EVT using a Willis covered stent in two institutions from March 2013 to March 2018. Clinical observations, angiographic characteristics, and procedural and follow-up outcomes were retrospectively evaluated. Conclusion Our initial results demonstrate that reconstructive EVT using a Willis covered stent provides a viable approach to treat ICA BBAs. However, an expanded clinical evaluation and larger cohort are needed to confirm the results. | Yi Gu Binxian Gu Yongdong Li Yueqi Zhu Haitao Lu Liming Wei Mo Chen Minhua Li Jieqing Wan Wu Wang | 2018 | Journal of Interventional Medicine2018,1,3: | 8 |
| 13 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021,14,6: | 8 |
| 14 | Tunable Schottky barrier width and enormously enhanced photoresponsivity in Sb doped SnS2 monolayer显示文摘Doping, which is the intentional introducti on of impurities into a material, can improve the metal-semiconductor interface by reducing Schottky barrier width. Here, we prese nt high-quality two-dime nsional SnS2 nano sheets with well-controlled Sb dopi ng concen tration via direct vapor growth approach and following micromechanical cleavage process. X-ray photoelectro n spectroscopy (XPS) measureme nt dem on strates that Sb contents of the doped samples are approximately 0.22%, 0.34% and 1.21%, respectively, and doping in duces the upward shift of the Fermi level with respect to the pristi ne SnS2. Tran smissio n electro n microscopy (TEM) characterizatio n exhibits that Sb-doped SnS2 nano sheets have a high-quality hexagonal symmetry structure and Sb element is uniformly distributed in the nano sheets. The phototra nsistors based on the Sb-doped SnS2 mono layers show n-type behavior with high mobility which is one order of magn itude higher than that of pristi ne SnS2 phototra nsistors. The photorespo nsivity and exter nal quantum efficiency (EQE) of Sb-S nS2 mono layers phototra nsistors are approximately three orders of magnitude higher than that of pristine SnS2 phototransistor. The results suggest that the method of reducing Shottky barrier width to achieve high mobility and photoresp on sivity is effective, and Sb-doped SnS2 mono layer has significant potential in future nanoelectronic and optoelectronic applications. | Junchi Liu Xiao Liu Zhuojun Chen Lili Miao Xingqiang Liu Bo Li Liming Tang Keqiu Chen Yuan Liu Jingbo Li Zhongming Wei Xidong Duan | 2019 | Nano Research2019,12,2: | 7 |
| 15 | GENETIC DIVERSITY AMONG CHINESE SIKA DEER (CERVUS NIPPON) POPULATIONS AND RELATIONSHIPS BETWEEN CHINESE AND JAPANESE SIKA DEER显示文摘Sika deer (Cervus nippon) is a cervid endemic to mainland and insular Asia and endan-gered. We analyzed variation in the mitochondrial DNA (mtDNA) control region for four subspecies to understand the genetic diversity, population structure and evolutionary history in China. 335 bp were se-quenced and eight haplotypes were identified based on 25 variable sites among the populations. Sika deer in China showed lower genetic diversity, sug-gesting a small effective population size due to habi-tat fragmentation, a low number of founder individu-als, or the narrow breeding program. AMOVA analy-sis indicated that there was significant genetic subdi-vision among the four populations, but no correlation between the genetic and geographic distance. Phy-logenetic analyses also revealed that Chinese sika deer may be divided into three genetic clades, but the genetic structure among Chinese populations was inconsistent with subspecies designations and pre-sent geographic distribution. Including the sequence data of Japanese sika deer, the results indicated that Chinese populations were more closely related to Southern Japanese populations than to the Northern Japanese one, and the Taiwan population was closer to populations of Northeastern China and Sichuan than to those of Southern China. | Lü Xiaoping WEI Fuwen LIMing YANG Guang LIU Hai | 2006 | Chinese Science Bulletin2006,51,4: | 7 |
| 16 | HQ-Tree: A Distributed Spatial Index Based on Hadoop显示文摘In this paper,we propose a novel spatial data index based on Hadoop:HQ-Tree.In HQ-Tree,we use PR QuadTrec to solve the problem of poor efficiency in parallel processing,which is caused by data insertion order and space overlapping.For the problem that HDFS cannot support random write,we propose an updating mechanism,called 'Copy Write',to support the index update.Additionally,HQ-Tree employs a two-level index caching mechanism to reduce the cost of network transferring and I/O operations.Finally,we develop MapReduce-based algorithms,which are able to significantly enhance the efficiency of index creation and query.Experimental results demonstrate the effectiveness of our methods. | FENG Jun TANG Zhixian WEI Mian XU Liming | 2014 | China Communications2014,11,7: | 5 |
| 17 | Ability of lower teardrop edge to restore anatomical hip center height in total hip arthroplasty显示文摘 | Lu Yufeng Cheng Liming Guo Wanshou Yu Qingsheng Gao Fuqiang Zhang Qidong Liu Zhaohui Sun Wei Shi Zhencai | 2014 | Chinese Medical Journal2014,,22: | 5 |
| 18 | A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria(Microcystis) in lake sediments显示文摘A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer,the CytoSense.For cell enumeration,diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells.An optimized procedure suggested that 5 mg dw(dry weight)/mL dilution combined with 200 W × 2 min sonication yielded the highest counting efficiency.Under the optimized determination conditions,the quantification limit of this protocol was 3.3×10 4 cells/g dw.For colony analysis,Microcystis were isolated from the sediment by filtration.Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 μm in length.Moreover,the relationship between colony size and cell number was determined for three Microcystis species,including Microcystis flos-aquae,M.aeruginosa and M.wessenbergii.Regression formulas were used to calculate the cell numbers in differentsized colonies.The developed protocol was applied to field sediment samples from Lake Taihu.The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis.This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium. | Quan Zhou Wei Chen Huiyong Zhang Liang Peng Liming Liu Zhiguo Han Neng Wan Lin Li Lirong Song | 2012 | Journal of Environmental Sciences2012,24,9: | 5 |
| 19 | Research progress on solidification structure of alloys by synchrotron X-ray radiography: A review显示文摘The synchrotron radiation technology has recently emerged as a powerful tool to characterize the real-time microstructure evolution during solidification of alloys.Compared with other methods,the synchrotron radiation technology,along with its unique advantages of strong brightness,high energy,excellent resolution,and good monochromaticity,allows for capturing the dendrite evolution behavior of alloys in real time and can be dynamically coordinated with high-resolution CCD(Charge-coupled Device)imaging systems.This paper briefly reviews the recent advances in developing synchrotron radiation for solidification of alloys with low,medium,and high melting points,and under the external electric,magnetic,and ultrasonic fields.Furthermore,a series of microstructural features and behaviors such as dendrite morphology,growth orientation,dendrite fracture,and rotation are described in detail.Finally,the development trends and application prospects of synchrotron radiation technology in alloy solidification are forecasted. | Yongbiao Wang Sensen Jia Mingguang Wei Liming Peng Yujuan Wu Xintian Liu | 2020 | Journal of Magnesium and Alloys2020,8,2: | 5 |
| 20 | Achieving ultra-high strength in Mg-Gd-Ag-Zr wrought alloy via bimodal-grained structure and enhanced precipitation显示文摘Mg-13.1 Gd-1.6 Ag-0.4 Zr(wt%)alloy was either iso-thermally extruded at 350℃ or differentialthermally extruded with respectively pre-heated billet at 500℃ and die at 350℃.The iso-thermal extrusion leads to a near fully recrystallized structure and a[0001]//ED(extrusion direction)texture.In contrast,the differential-thermally extruded alloy develops a bimodal-grained structure composed of fine equiaxed recrystallized grains and coarse elongated unrecrystallized grains with a0110//ED texture.The differential-thermally extruded alloy has a higher number density of precipitates after postextrusion ageing than that of the iso-thermally extruded counterpart.Moreover,precipitation in the differential-thermally extruded alloy is further enhanced with cold rolling before ageing.Finally,the alloy obtains room temperature tensile yield strength of 421 MPa and ultimate tensile strength of 515 MPa via differential-thermal extrusion,cold rolling and ageing,mainly ascribed to the coupled strengthening from the bimodal-grained structure and enhanced precipitation.Strength of the alloy is noticeably higher than those of Mg-Gd(-Y)-Ag extruded alloys with similar compositions reported previously and is comparable to those of other high-strength Mg wrought alloys.The findings suggest that differentialthermal extrusion plus strain ageing is a suitable approach for achieving high strength in age-hardenable Mg alloys. | Yu Zhang Wei Rong Yujuan Wu Liming Peng | 2020 | Journal of Materials Science & Technology2020,54,19: | 5 |