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| 1 | Genetic correction of p-thalassemia patient-specific iPS cells and its use in improving hemoglobin production in irradiated SCID mice显示文摘 | Yixuan Wang Chen-Guang Zheng Yonghua Jiang Jiqin Zhang Jiayu Chen Chao Yao Qingguo Zhao Sheng Liu Ke Chen Juan Du Ze Yang Shaorong Gao | 2012 | Cell Research2012，22，4： | 27 |
| 2 | MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions显示文摘在氮(N) 奢侈条件下面的住宿实质地减少庄稼产量和种子质量。然而,植物住宿抵抗的分子的机制仍然保持大部分不清楚,特别在玉米。我们这里报导 ZmmiR528 的表示, monocot 特定的 microRNA,被 N 导致 ? 奢侈但是由 N 缺乏减少了。我们由 thioacidolysis 和 N 奢侈显著地减少的乙酰溴化物分析证明在玉米的木质素以及它的全部的内容的 H, G,和 S 单体的产生射击。我们进一步示威 ? 那 ZmLACCASE3 (ZmLAC3 ) 编码包含铜的 laccases,和 ZmLACCASE5 (ZmLAC5 ) 是 ZmmiR528 的目标。在杂交显示出的 situ, ZmmiR528 是,这主要在玉米表示了脉管的纸巾。显著地增加的 ZmLAC3 的 ZmmiR528 或 overexpression 击倒木质素内容和玉米的树皮透度计抵抗起源。相反,转基因的玉米植物 overexpressing ZmmiR528 减少了木质素内容和树皮透度计抵抗并且对在揭示的 N 奢侈定序 conditions.?RNA 分析下面的住宿敏感那 ZmPAL7 和 ZmPAL8 是在转基因的玉米线 downregulating ZmmiR528 的 upregulated。在 N 奢侈条件下面, ZmPALs 的表示层次比在野类型在 ZmmiR528 击倒的线是高得多的并且 ? 转基因的玉米线 overexpressing ZmmiR528。一起拿,这些结果显示由调整 ZmLAC3 和 ZmLAC5 的表示, ZmmiR528 在 N 奢侈条件下面影响玉米住宿抵抗。 | Qing Sun Xiaogang Liu Juan Yang Wenwen Liu Qingguo Du Hongqiu Wang Chunxiang Fu Wen-Xue Li | 2018 | Molecular Plant2018，11，6： | 21 |
| 3 | Zircon U-Pb Geochronological Constraints on Rapid Exhumation of the Mantle Peridotite of the Xigaze Ophiolite, Southern Xizang(Tibet)显示文摘The Xigaze ophiolite crops out in the central segment of the Yarlung Zangbo suture zone,southern Tibet(Fig.1).It is characterized by large amounts of ultramafic units with minor mafic rocks.The mafic rocks consist of gabbros, | LIU Tong WU Fuyuan ZHANG Liangliang ZHAI Qingguo LIU Chuanzhou JI Wenbin Ji ZHANG Chang XUYang | 2016 | Acta Geologica Sinica(English Edition)2016，90，S1： | 12 |
| 4 | Experimental studies on the P-T stability conditions and influencing factors of gas hydrate in different systems显示文摘The P-T stability conditions of gas hydrate in different systems (i.e., solution, silica sand, and marine sediment) were studied using multi-step decomposition method with our experimental equipment. The effects of different ions with various concentrations and sediment grains on the P-T stability conditions of gas hydrate were investigated. The results show that different ions have different influences on the phase equilibrium of gas hydrate. However, the influence of ions is in a similar trend: the larger the concentration, the bigger the P-T curve shifts to the left. For the silica sand, the influence of pore capillarity of coarse particles (> 460 μm) can be negligible. The P-T curve measured in coarse silica is in agreement with that in pure water. However, the influence of pore capillarity of fine particles (< 35 μm) is significant. The maximum reduction value of temperature is 1.5 K for methane hydrate under stable state. The sediment from the South China Sea significantly affects the P-T stability conditions of methane hydrate, with an average reduction value of 1.9 K within the experimental conditions. This is mainly the result of both the pore water salinity and the pore capillarity of sediment. Because the pore water salinity is keeping diluted by the fresh water released from hydrate dissociation, the measured P-T stability points fall on different P-T curves with the decreasing salinity. | LIU ChangLing YE YuGuang SUN ShiCai CHEN Qiang MENG QingGuo HU GaoWei | 2013 | Science China Earth Sciences2013，56，4： | 11 |
| 5 | Effect of electro-acupuncture on gene expression in heart of rats with stress-induced pre-hypertension based on gene chip technology显示文摘OBJECTIVE:To explore electro-acupuncture's(EA's)effect on gene expression in heart of rats with stress-induced pre-hypertension and try to reveal its biological mechanism based on gene chip technology.METHODS:Twenty-seven Wistar male rats were randomly divided into 3 groups.The stress-induced hypertensive rat model was prepared by electric foot-shocks combined with generated noise.Molding cycle lasted for 14 days and EA intervene was applied on rats in model + EA group during model preparation.Rat Gene 2.0 Sense Target Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) method.RESULTS:Compared with blank control group,390 genes were changed in model group;compared with model control group,330 genes were changed in model + EA group.Significance analysis of gene function showed that the differentially expressed genes are those involved in biological process,molecular function and cellular components.RT-PCR result of the screened key genes is consistent with that of gene chip test.CONCLUTION:EA could significantly lower blood pressure of stress-induced pre-hypertension rats and affect its gene expression profile in heart.Genes that related to the contraction of vascular smooth muscle may be involved in EA's anti-hypertensive mechanism. | Guo Yan Xie Xiaojia Guo Changqing Wang Zhaoyang Liu Qingguo | 2015 | Journal of Traditional Chinese Medicine2015，35，3： | 10 |
| 6 | Expression of HMGB2 indicates worse survival of patients and is required for the maintenance of Warburg effect in pancreatic cancer显示文摘高活动性组蛋白质(HMG ) 是第二很丰富的染色质蛋白质并且在活跃或不活跃的染色质域的建立施加全球 genomic 函数。通过和 nucleosomes,抄写因素,改变 nucleosome 机器和 histones 的相互作用, HMG 家庭蛋白质贡献响应快速的环境变化抄写调节的罚款。哺乳动物的高活动性组 B (HMGB ) 被领域用一条长酸的尾巴跟随了的二个双人脚踏车 HMG 盒子描绘。最近的研究证明 HMGB 的那高表情在许多癌症被发现了,例如前列腺,肾,卵巢、胃的癌症。然而,他们在胰腺的癌症的角色很少被报导了。在这研究,我们估计了 HMGB 蛋白质的诊断、预示的值,包括 HMGB1, HMGB2,和 HMGB3,在从癌症染色体地图集(TCGA ) 数据集的胰腺的癌症。我们的结果证明 HMGB2 在胰腺的癌症预言了差的预后。在 vitro,研究证明 silencing HMGB2 禁止了房间增长和生存能力。机械学地,我们的结果证明 silencing HMGB2 减少了组织缺氧可诱导的因素 1 (HIF1 ) 蛋白质水平和禁止的调停 HIF1 的 glycolysis 处理。进一步的分析显示包括 GLUT1, HK2,和 LDHA,指向 HIF1 的 glycolytic 基因都是预示的因素并且断然与 HMGB2 表示相关。一起拿,我们为胰腺的癌症发现了新预示、预兆的标记,并且在癌症在 glycolytic 控制使 HMGB2 的新奇函数清楚些。 | Xin Cai Hongjian Ding Yanxia Liu Gaofeng Pan Qingguo Li Zhen Yang Weiyan Liu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，2： | 9 |
| 7 | Sini powder ameliorates the inflammatory response in rats with stress-induced non-alcoholic fatty liver disease by inhibiting the nuclear factor kappa-B/pyrin domain-containing protein 3 pathway显示文摘OBJECTIVE: To evaluate the effectiveness of Sini powder for the treatment of non-alcoholic fatty liver disease(NAFLD) in rats and the molecular mechanisms involved.METHODS: A rat model of stress-induced NAFLD was established by a combination of long-term tethering and feeding of a high-fat, high-calorie diet. These rats were then intragastrically administered with either simvastatin, Sini powder, or vehicle for 1 week. The body mass and field test scores for each group were recorded weekly. Serum aspartate aminotransferase and alanine aminotransferase activities, and triglyceride, total cholesterol,and free fatty acid concentrations were measured.Liver tissue histopathology was examined on hematoxylin and eosin-stained paraffin sections and oil red O-stained frozen sections. The hepatic m RNA expression of nuclear factor kappa-B(NF-κB),NOD-, LRR-, and pyrin domain-containing protein 3(NLRP3), apoptosis-associated speck-like protein containing CARD(ASC), and caspase-1 were measured by reverse transcription-polymerase chain reaction(RT-PCR). The hepatic protein concentrations of NF-κB and NLRP3, ASC, caspase-1, interleukin-1β(IL-1β), interleukin-6(IL-6), and the serum concentrations of IL-1β and IL-6 were measured by enzyme-linked immunosorbent assay.RESULTS: Compared with the Blank group, rats in the Compound model group showed significant pathologic manifestations of NAFLD, and the expression of NF-κB, NLRP3, ASC, caspase-1, IL-1βand IL-6 were significantly higher(all P < 0.01). Both simvastatin and Sini powder significantly ameliorated the NAFLD pathology and the abnormal expression of NF-κB, NLRP3, ASC, caspase-1, IL-1β, and IL-6(all P < 0.01).CONCLUSION: Sini powder inhibits the inflamma-tory response in rats with NAFLD, which is mediated by NF-κB/NLRP3, IL-1β, and IL-6, reduces the effects of psychological stress, and improves lipid metabolism. Therefore, Sini powder may be effective for the treatment of stress-related NAFLD through multiple mechanisms. | Mu Jie Cheng Fafeng Wang Qingguo Wang Xueqian Zhu Wenxiang Ma Chongyang Yin Xiangjun Ren Beida Lian Yajun Du Xin Zhang Haixia Liu Shuling Zhang Shuang | 2020 | Journal of Traditional Chinese Medicine2020，40，2： | 9 |
| 8 | Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation显示文摘在房间期间,分割要求忠诚有丝分裂的锭子汇编和染色体分离的基因信息的稳定的传播。运用了 GTPase 戏在有丝分裂的锭子汇编的一个关键角色。然而,在锭子的 Ran-GTP 的一个化学坡度的产生怎么被联合到有丝分裂的 translational 以后修正,从来没被描绘过。这里,我们解决了复杂结构与核苷酸版本因素 Mog1 跑了并且描出一个新奇有丝分裂特定的调整 acetylation 的 Ran-Mog1 相互作用在染色体分离期间。我们的指导结构的功能的分析表明那 Mog1 与 RCC1 竞争为以一种 GTP/GDP-dependent 方式变有约束力。生物化学的描述表明了那 Mog1 固定跑阻止 RCC1 有约束力、随后的 GTP 装载。令人惊讶地,跑了是 TIP60 的真正的底层,并且由 TIP60 的 Lys134 的 acetylation 解放 Mog1 从在有丝分裂期间变有约束力。重要地,这个得到 acetylation 的开关对 RCC1 变有约束力支持 Ran-GTP 的高水平,它为染色体排列是必要的。这些结果建立一以前 TIP60 由调节在提供 Ran-GTP 水平的 homeostatic 控制的规章的机制运用了的 uncharacterized 为在有丝分裂的染色体分离的受动器绑定。 | Xiaoling Bao Heng Liu Xing Liu Ke Ruan Yonshui Zhang Zhiyong Zhang Qi Hu Ying Liu Saima Akram Jiahai Zhang Qingguo Gong Wenwen wang Xiao Yuan Jian-Li Lingli Zhao Zhen Dou Ruijun Tian Xuebiao Yao Jihui Wu Yunyu Shi | 2018 | Journal of Molecular Cell Biology2018，10，1： | 8 |
| 9 | Identification and PID Control for a Class of Delay Fractional-order Systems显示文摘In this paper,a new model identification method is developed for a class of delay fractional-order system based on the process step response.Four characteristic functions are defined to characterize the features of the normalized fractionalorder model.Based on the time scaling technology,two identification schemes are proposed for parameters' estimation.The scheme one utilizes three exact points on the step response of the process to calculate model parameters directly.The other scheme employs optimal searching method to adjust the fractional order for the best model identification.The proposed two identification schemes are both applicable to any stable complex process,such as higher-order,under-damped/over-damped,and minimum-phase/nonminimum-phase processes.Furthermore,an optimal PID tuning method is proposed for the delay fractionalorder systems.The requirements on the stability margins and the negative feedback are cast as real part constraints(RPC)and imaginary part constraints(IPC).The constraints are implemented by trigonometric inequalities on the phase variable,and the optimal PID controller is obtained by the minimization of the integral of time absolute error(ITAE) index.Identification and control of a Titanium billet heating process is given for the illustration. | Zhuoyun Nie Qingguo Wang Ruijuan Liu Yonghong Lan | 2016 | IEEE/CAA Journal of Automatica Sinica2016，3，4： | 6 |
| 10 | Robust superhydrophobic polyurethane sponge functionalized with perfluorinated graphene oxide for efficient immiscible oil/water mixture, stable emulsion separation and crude oil dehydration显示文摘In recent years, graphene oxide(GO), prepared by the modified Hummers’ method, and its derivatives have become a focus of research owing to their outstanding physical and chemical properties and low cost. Drawing inspiration from the mussel protein,a facile and environmentally-friendly method was employed to fabricate superhydrophobic/superoleophilic reduced graphene oxide(rGO) derivative. The preparation comprises two steps: coating GO nanosheets with polydopamine(PDA) and subsequent reaction with 1H,1H,2H,2H-perfluorodecanethiol. Due to the excellent adhesive ability of PDA, the resulting f PDA modified rGO nanosheets(rGO-f PDA) were firmly immobilized onto polyurethane(PU) sponge skeleton by a simple drop-coating method. The as-prepared rGO-f PDA functionalized sponge exhibited superhydrophobic behavior with a water contact angle of 162°±2°, high organic adsorption capacity, recyclability and stable oil/water separation behavior under different acidic/alkaline conditions. Due to its facile fabrication technique and outstanding properties, the superhydrophobic-superoleophilic PU-rGOf PDA sponge holds great promise as an oil adsorbent for cleaning up large-scale pollution of oil and organic solvents, and dehydrating crude oil. | CAO Ning GUO JingYu BOUKHERROUB Rabah SHAO QingGuo ZANG XiaoBei LI Jin LIN XueQiang JU Hong LIU EnYang ZHOU ChaoFan LI HuiPing | 2019 | Science China(Technological Sciences)2019，62，9： | 6 |
| 11 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 12 | Effects of electroacupuncture at Taichong(LR 3) and Baihui(DU 20)on cardiac hypertrophy in rats with spontaneous hypertension显示文摘OBJECTIVE: To investigate the effects of electroacupuncture(EA) at Taichong(LR 3) and Baihui(DU 20)on myocardial hypertrophy in spontaneously hypertensive rats(SHRs).METHODS: Thirty-six SHRs were randomly assigned to model, EA, and Losartan groups, with twelve rats per group. Twelve Wistar Kyoto rats were selected as the normal control group. Systolic blood pressure(SBP) and cardiac function were measured in all rats.Expression levels of factors associated with the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR) pathway were evaluated by Western blotting and real-time PCR.Pathological changes of the heart tissue were observed by hematoxylin-eosin staining.RESULTS: After treatment, enhanced SBP was significantly decreased in the EA and Losartan groups compared with the model group(P < 0.01). Echocardiographic and morphological analyses revealed that enhanced end-diastolic interventricular septal thickness and left ventricular posterior wall thickness, as well as ratio of left ventricular weight to body weight were markedly diminished in the EA and Losartan groups(P < 0.01 or P < 0.05), while reduced left ventricular end-diastolic dimension and left ventricular ejection fraction were significantly ameliorated(P < 0.01). Real-time PCR and western blotting analyses showed that the expression levels of PI3K,Akt, and mT OR in SHRs were significantly up-regulated by EA and Losartan(P < 0.01), while the expression levels of PTEN and ANP were down-regulated(P < 0.01).CONCLUSION: EA at Taichong(LR 3) and Baihui(DU20) inhibited the development of cardiac hypertrophy and improved the cardiac function in SHRs, possibly through regulation of the PI3K/Akt/mTOR signalling pathway. | Ji Zhi Zhang Yue Liang Jingrong Wu Jiaojuan Jia Wenrui Wang Zhaoyang Liu Qingguo | 2019 | Journal of Traditional Chinese Medicine2019，39，4： | 6 |
| 13 | Upregulated KPNA2 promotes hepatocellular carcinoma progression and indicates prognostic significance across human cancer types显示文摘Hepatocellular carcinoma(HCC)is one of the most aggressive cancers worldwide.Identification of the molecular mechanisms underlying the development and progression of HCC is particularly important.Here,we demonstrated the expression pattern,clinical significance,and function of Karyopherin α2(KPNA2)in HCC.The expression of KPNA2 was upregulated in tumor tissue and negatively associated with the survival time,and a significant correlation between KPNA2 expression and aggressive clinical characteristics was established.Both in vitro and in vivo experiments demonstrated that knockdown of KPNA2 reduced migration and proliferation capacities of HCC cells,while over-expression of KPNA2 increased these malignant characteristics.The analysis of the Cancer Genome Atlas cohorts also reveals that high-KPNA2 expression is associated with poor outcome in multiple cancer types.In addition,gene sets enrichment analysis exhibited cell cycle and DNA replication as the top altered pathways in the high-KPNA2 expression group in HCC and other two cancer types.Overall,this study identified KPNA2 as a potential diagnostic and prognostic biomarker in HCC and other neoplasms,probably by regulating cell cycle and DNA replication. | Xinggang Guo Zhiheng Wang Jianing Zhang Qingguo Xu Guojun Hou Yuan Yang Chuanpeng Dong Gang Liu Chenhua Liang Lei Liu Weiping Zhou Hui Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 6 |
| 14 | Single W18O49 nanowires: A multifunctional nanoplatform for computed tomography imaging and photothermal/ photodynamic/radiation synergistic cancer therapy显示文摘联合治疗是通常基于复杂 nanostructures 的利用,多重部件为 photothermal 作为相片热的精力变换器, photo-sensitizers,或剂量 intensifiers 工作的有希望的癌症治疗策略治疗(PTT ) ,光力学的治疗(太平洋夏季时间) ,或放射治疗(RT ) 。在这研究, ultrathin 钨氧化物 nanowires (W 18 O 49) 用一条 solvothermal 途径被综合并且作为多功能的 theranostic nanoplatform 检验了。在 vitro 并且在 vivo,分析证明这些 nanowires 能导致广泛的热 -- 并且汗衫在 980 nm 在附近下面的癌症房间的调停氧的损坏红外线(NIR ) 激光刺激。他们也被显示作为加强在放射治疗期间局部地并且有选择地提高放热精力免职的代理人的放射剂量工作。独自比作导致 NIR 的 PTT/PDT 和 RT, W 18 O 49-based synergistic tri 形式的治疗根除了异种皮移植肿瘤和没有复发被观察在以内一 9 月列在后面起来。而且,钨元素的强壮的 X 光检查变细能力(Z = 74, 4.438 厘米 2???? ?? ?? 佦 ? 畳 ?? 猯扵￣?? | Jianjian Qiu Qingfeng Xiao Xiangpeng Zheng Libo Zhang Huaiyong Xing Dalong Ni Yanyan Liu Shengjian Zhang Qingguo Ren Yanqing Hua Kuaile Zhao Wenbo Bu | 2015 | Nano Research2015，8，11： | 5 |
| 15 | Advances and prospects of PVDF based polymer electrolytes显示文摘Exploring highly foldable batteries with no safety hazard is a crucial task for the realization of portable,wearable,and implantable electric devices.Given these concerns,developing solid-state batteries is one of the most promising routes to achieve this aspiration.Because of the excellent flexibility and processability,polyvinylidene fluoride(PVDF) based electrolytes possess great potential to pack high energy density flexible batteries,however,suffers the various intrinsic shortcomings such as inferior ionic conductivity,a high degree of crystallinity,and lack of reactive groups.Clearing the progress of the present state and concluding the specific challenges faced by PVDF based electrolytes will help to develop PVDF based polymer batteries.In this review,we summarize the recent progress of gel polymer electrolytes and all solid polymer electrolytes based on PVDF.The ion transport mechanisms and preparation methods of PVDF based electrolytes are briefly introduced.Meanwhile,the current design principle and properties of electrolytes are highlighted and systematically discussed.Some peculiar modified strategies performed in lithium-sulfur batteries and lithium-oxygen batteries are also included.Finally,this review describes the challenges and prospects of some solid-state electrolytes to provide strategies for manufacturing high-performance PVDF electrolytes aimed at practical application with flexible requirements. | Yixin Wu Yu Li Yang Wang Qian Liu Qingguo Chen Minghua Chen | 2022 | Journal of Energy Chemistry2022，31，1： | 5 |
| 16 | Possible mechanisms underlying treatment of Alzheimer’s disease with Traditional Chinese Medicine: active components, potential targets and synthetic pathways of Bulao Elixir显示文摘OBJECTIVE: To elucidate the mechanisms underlying the treatment of Alzheimer’s disease(AD) with Traditional Chinese Medicine(TCM), by examining the active components, potential targets and synthetic pathways of Bulao Elixir(BLE).METHODS: The Absorption, Distribution, Metabolism, Excretion(ADME)/Toxicology(T) calculation was used to screen the active components of Bulao Elixir. Based on the TCM Systems Pharmacology Analysis Platform(TCMSP database) and a text mining tool(GoPubMed database), we predicted and screened the active components of Bulao Elixir and its therapeutic targets for AD. Using the Database for Annotation, Visualization and Integrated Discovery(DAVID), we obtained the targets for AD. Cytoscape software was used to establish a network map of the active component-target and target-pathway of Bulao Elixir. Gene function, related biological processes and signaling pathways were analyzed using the DAVID database.RESULTS: Twelve active components were selected from 196 components of Bulao Elixir. Among 2209 targets, 102 effective targets were selected, and 30 important targets were identified via matching with the disease targets. After further analysis, 14 core targets were identified. Enrichment analysis revealed that most of these important targets were involved in multiple biological processes, including apoptosis, inflammatory reactions, and cell regulation cycles. The synthetic pathways for AD treatment were identified after analyzing and confirming the relevant pathways, providing potentially useful information for diagnosis and treatment methods for AD.CONCLUSION: The current study elucidated the potential treatment mechanisms of Bulao Elixir in AD using network pharmacology, providing a foundation for further clarification of its treatment targets. | Ren Beida Cheng Fafeng Wang Xueqian Wan Yuxiang Ji Wenting Du Xin Zhang Shuang Liu Shilling Ma Chongyang Xiong Yiliang Hao Gaoting Wang Qingguo | 2020 | Journal of Traditional Chinese Medicine2020，40，3： | 4 |
| 17 | Effects of different acupuncture manipulations on protein expression in the parietal cortex of spontaneously hypertensive rats显示文摘Objective: To analyze the effects of twirling reinforcing and reducing manipulations on protein expression in parietal cortex of spontaneously hypertensive rats(SHRs), and elucidate the main mechanisms and differences between two manipulations in hypertension treatment.Methods: Rats were randomly divided into the control, model, twirling reinforcing manipulation(TRFM),and twirling reducing manipulation(TRDM) groups. The control and model groups received catch and fixation stimulations once a day for 14 days. The TRFM and TRDM groups were intervened once a day for 20 min for 14 days. On days 0, 2, 6, 10, and 14 after acupuncture, rat systolic blood pressures(SBPs) were measured. Differential protein(DP) expression in the rat parietal cortices was detected. Thereafter, GO functional significance and KEGG pathway enrichment analyses were performed.Results: Compared with the model group, SBP of rats in the TRDM and TRFM groups decreased on days 6 and 10 of acupuncture, respectively(P=.009;P <.001). Moreover, SBP of the TRDM group was significantly lower than that of the TRFM group on days 10 and 14 of acupuncture(P=.015;P=.013).Compared with control group, 601 and 1040 DPs were up-and downregulated, respectively, in the model group. Compared with model group, 44 and 28 up-and downregulated DPs were expressed, respectively,in the TRFM group. Compared with model group, expression of 616 and 427 up-and downregulated DPs,respectively, was found in the TRDM group. After combining the results of GO and KEGG enrichment analyses, five and one pathways were found to be related to the central antihypertensive mechanism of the parietal cortex during twirling reducing and reinforcing manipulations, respectively.Conclusion: TRDM showed a more effective antihypertensive effect on SHRs than TRFM;this antihypertensive effect was related to the regulation of different proteins and their biological functions. | Jiaojuan Wu Tianxiao Zeng Jingrong Liang Xudong Zhang Qi Xie Taotao Lv Pui Yee Wong Zhi Ji Qingguo Liu | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，3： | 4 |
| 18 | Mechanism underlying effect of Chaihu Shugan San(柴胡疏肝散)on major depressive disorder:a network pharmacology-based study显示文摘OBJECTIVE:To investigate the mechanism underpinning the effect of Chaihu Shugan San(柴胡疏肝散,CHSGS)on major depressive disorder(MDD).METHODS:We searched the compound components of from seven herbal ingredients of CHSGS from TCMSP,SymMap,ETCM,NPASS databases,and the chemical structure of the compound from PubChem,and collected the compound targets from TCMSP and TargetNet databases,and MDD-related targets from DiseaseGene Network.We established protein-protein interaction in the STRING database.Through gene mapping,topology analysis and enrichment analysis,the core targets and pathways of CHSGS for MDD,and the involved biological processes(BP),cell components(CC),and molecular functions(MF)were predicted.RESULTS:We collected a total of 1135 CHSGS compounds.After screening by ADME standards and the five rules of Ribinski,we obtained 99 different chemical components with different chemical structures,and related targets of 183 different CHSGS compounds.In the DiseaseGene Network,a total of 740 relevant targets for MDD were collected.Through gene mapping and topological analysis,62 related targets of CHSGS for MDD,24 targets with topological Chinese herbal medicine were obtained.Through enrichment analysis,10 relevant pathways and 3 core pathways were obtained with the involvement of 127 BP,27 CC,and 43 MF.CONCLUSION:There are multiple targets and signaling pathways are involved in the action of CHSGS in the treatment of MDD. | MU Jie ZHANG Shuang TANG Feifei LIU Shuling ZHANG Qianyi DU Qianlei WANG Xueqian WANG Qingguo CHENG Fafeng | 2021 | Journal of Traditional Chinese Medicine2021，41，2： | 4 |
| 19 | Harmonic frequency mixing using high T_c superconductor Josephson junction mounted on pulse tube cryocooler显示文摘A frequency mixing system including microwave coupling and intermediate frequency (IF) measurement arrangements is designed. In lieu of liquid nitrogen, a pulse tube cryocooler is used to cool the whole system. With YBa2Cu3O7/Yttrium stabilized zirconia (YBCO/YSZ) bicry-stal Josephson junction as the mixing element, 36th harmonic frequency mixing at the 8 mm waveband is obtained. | Weiwei Xu Qingguo Liu Gangdong Zhou Zhengming Ji Peiheng Wu | 2001 | Chinese Science Bulletin2001，46，18： | 4 |
| 20 | Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq显示文摘Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid genome.In this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations.We applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic traits.Candidate causative genes at these loci were detected based on functional clues.Taken together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient way.The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html. | Mengjiao Chen Weijuan Fan Feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli Zheng Qi Zhang Jiaxin Chen Jie Qiu Xin Wei Ziru Chen Peng Zhang Dong Pei Jun Yang Xuehui Huang | 2021 | Molecular Plant2021，14，4： | 4 |
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