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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Urotensin Ⅱ accelerates cardiac fibrosis and hypertrophy of rats induced by isoproterenol显示文摘目的:学习是否 urotensin II ( UII ),有势力 vasoconstrictive 肽,在心脏的肥大的发展和老鼠的 fibrogenesis 的 isinvolved 由 isoproterenol ( ISO )导致了 .Methods :三十只男 Wistar 老鼠随机被划分成 1 是的 3 groups.Group healthycontrol 组,组 2 是 ISO 组,并且组 3 是 ISO+UTI group.In 组 2 和 3 , ISO ( 5mg 中心点kg^(-1)中心点d^(-1))被给( sc )为 3 也是的 7 d.Group 从前每日在第一天内的 givenUTI [ 3 nmol/kg ( 5 ug/kg )。 | Yong-gang ZHANG Yu-guang LI Bao-guo LIU Rui-hong WEI Dong-ming WANG Xue-rui TAN Ding-fang BU Yong-zheng PANG Chao-shu TANG | 2007 | Acta Pharmacologica Sinica2007，28，1： | 36 |
| 3 | Predictive factors and unfavourable prognostic factors of interstitial lung disease in patients with polymyositis or dermatomyositis: a retrospective study显示文摘背景空隙的肺疾病(ILD ) 是在 polymyositis (下午) 和影响预后并且在治疗要求一条更好攻击的途径的 dermatomyositis (DM ) 的严肃的肺复杂并发症。这研究在最新诊断的下午, DM 和 amyopathic DM (ADM ) 调查了流行,特征,预兆的因素和 ILD 的不利预示的因素。到 2008 年 12 月, 197 连续下午的医药记录和在孙中山大学的第二所隶属于的医院的 DM 病人的从 2000 年 1 月的方法被考察排除重叠,少年,和联系恶意的案例。病人被分到 ILD (69 个病人) 和一个 non-ILD 组(128 个病人) 。临床的特征,实验室调查结果,和预后被比较。结果 multivariate 分析在发作显示了那老年(或 1.033 , 95%CI 1.009-1.058 , P=0.007 ),发烧(或 4.109 , 95%CI 1.926-8.767 , P < 0.001 )并且关节炎/关节痛(或 2.274 , 95%CI 1.101-4.695 , P=0.026 )是为在排除 anti-Jo-1 以后在 PM/DM 开发 ILD 的独立预兆的因素。关于 anti-Jo-1,发烧(或 4.912, 95%CI 2.121-11.376, P < 0.001 ) 与 ILD 被联系。在 ILD 病人的差的幸存与 ILD 临床的子集被联系( RR 0.122 , 95%CI 0.049-0.399 , P < 0.001 ), ADM/DM/PM-ILD ( RR 0.140 , 95%CI 0.031-0.476 , P=0.002 ),心脏的参与( RR 4.654 , 95%CI 1.391-15.577 , P=0.013 )并且浆液白朊水平( RR 0.910 , 95%CI 0.831-0.997 , P=0.042 )。与发烧介绍了的结论病人趋于有 PM/DM-associated ILD 的更高的频率。一个 Hamman-Rich-like 演讲, ADM-ILD,心脏的参与和 hypoalbuminemia 是在 ILD-PM/DM 的差的预示的因素。 | JI Su-yun ZENG Fan-qin GUO Qing TAN Guo-zhen TANG Hong-feng LUO Yi-jin TANG Zeng-qi HAN Yan-fang | 2010 | Chinese Medical Journal2010，，5： | 33 |
| 4 | Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System显示文摘 | Lu, Yuming Ye, Xiao Guo, Renming Huang, Jing Wang, Wei Tang, Jiuyou Tan, Longtao Zhu, Jian-kang Chu, Chengcai Qian, Yangwen | 2017 | Molecular Plant2017，10，9： | 28 |
| 5 | PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy显示文摘Mitophagy 是为损坏线粒体的特定的消除的选择 autophagy 的一种重要类型。(PINK1 )导致 PTEN 的通常认为的 kinase 蛋白质 1 催化 ubiquitin (Ub ) 的 phosphorylation 在 PINK1-Parkin-mediated mitophagy 的发作起一个关键作用。(PTEN-L ) 磷酸酶和 tensin 相当或相同的事物(PTEN ) 长是 PTEN 的最新识别的 isoform,与到它的 N 终点的 173 氨基酸的增加。这里,我们报导 PTEN-L 是经由它对 phosphorylated ubiquitin 的蛋白质磷酸酶活动的 mitophagy 的一个新奇否定管理者。我们发现 PTEN-L 在外部 mitochondrial 膜(OMM ) 本地化,而 PTEN-L 的删除支持, PTEN-L 的 overexpression 禁止, mitophagy 由各种各样的损坏线粒体的代理人导致了。机械学地, PTEN-L 能够有效地阻止 Parkin mitochondrial translocation,减少 Parkin phosphorylation,维持它的关上的不活跃的符合构造,并且禁止它的 E3 ligase 活动。更重要地, PTEN-L 在 vivo 减少 phosphorylated ubiquitin (pSer65-Ub ) 的水平,并且在 vitro,磷酸酶试金经由它的蛋白质磷酸酶活动证实那 PTEN-L dephosphorylates pSer65-Ub,独立于它的类脂化合物磷酸酶功能。一起拿,我们的调查结果为 ubiquitin 作为蛋白质磷酸酶表明 PTEN-L 的新奇功能,它抵抗在 mitophagy 正式就职和 mitophagy 的最终的抑制导致前馈控制机制的阻塞的调停 PINK1 的 ubiquitin phosphorylation。因此,理解 PTEN-L 的这新奇功能在控制 mitophagy 的分子的难题提供一关键错过片,在包括象 Parkinsons 那样的 neurodegenerative 混乱的许多重要人的疾病的一个批评过程疾病。 | Liming Wang Yik-Lam Cho Yancheng Tang Jigang Wang Jung-Eun Park Yajun Wu Chunxin Wang Yan Tong Ritu Chawla Jianbin Zhang Yin Shi Shuo Deng Guang Lu Yihua Wu Hayden Weng-Siong Tan Pornteera Pawijit Grace Gui-Yin Lim Hui-Ying Chan Jingzi Zhang Lei Fang Hanry Yu Yih-Cherng Liou Mallilankaraman Karthik Boon-Huat Bay Kah-Leong Lim Siu-Kwan Sze Celestial T. Yap Han-Ming Shen | 2018 | Cell Research2018，28，8： | 25 |
| 6 | Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells显示文摘Liver fibrosis is a consequence of chronic liver disease,causing morbidity and mortality.Interleukin-33(IL-33)is a critical mediator of inflammation,which may be involved in the development of liver fibrosis.Here,we investigated the role of IL-33 in human patients and experimental bile-duct ligation(BDL)-induced fibrosis in mice.We report increased hepatic IL-33 expression in the murine BDL model of fibrosis and in surgical samples obtained from patients with liver fibrosis.Liver injury,inflammatory cell infiltration and fibrosis were reduced in the absence of the IL-33/ST2 receptor,and the activation of hepatic stellate cells(HSCs)was decreased in ST2-deficient mice.Recombinant IL-33 activated HSCs isolated from C57BL/6 mice,leading to the expression of IL-6,TGF-β,α-SMA and collagen,which was abrogated in the absence of ST2 or by pharmacological inhibition of MAPK signaling.Finally,administration of recombinant IL-33 significantly increased hepatic inflammation in sham-operated BL6 mice but did not enhance BDL-induced hepatic inflammation and fibrosis.In conclusion,BDL-induced liver inflammation and fibrosis are dependent on ST2 signaling in HSCs,and therefore,the IL-33/ST2 pathway may be a potential therapeutic target in human patients with chronic hepatitis and liver fibrosis. | Zhongming Tan Qianghui Liu Runqiu Jiang Long Lv Siamak S Shoto Isabelle Maillet Valerie Quesniaux Junwei Tang Wenjie Zhang Beicheng Sun Bernhard Ryffel | 2018 | Cellular & Molecular Immunology2018，15，4： | 24 |
| 7 | Prevalence and risk factors of fatty liver disease in Chengdu,Southwest China显示文摘BACKGROUND:Fatty liver disease(FLD)is increasingly recognized as one of the most common chronic liver diseases in China.This study aimed to investigate the prevalence and risk factors of FLD in Chengdu,Southwest China,and to provide a relevant basis for the prevention and intervention of FLD. METHODS:Altogether 9094 subjects(4721 men and 4373 women)of over 18 years old who had received a medical checkup in the West China Hospital of Sichuan University between January and December 2007 were evaluated for FLD.FLD was diagnosed by ultrasonography.Body mass index(BMI),height,body weight,blood pressure,fasting plasma glucose(FPG),triglycerides(TG),total cholesterol (TCh),alanine aminotransferase(ALT),hepatitis B surface antigen(HBsAg),high-density lipoprotein cholesterol (HDL-C),and low-density lipoprotein cholesterol(LDL-C) were measured using routine laboratory methods. RESULTS:The overall prevalence of FLD was 12.5%,which was more than 3-fold higher in males than in females (18.9%vs.5.7%,χ2=359.624,P<0.001).The prevalence increased with age in females and males of less than 50 years.The prevalence of alcoholic,suspected alcoholic,and non-alcoholic FLD was 2.6%,3.6%,and 6.3%,respectively. Multiple logistic regression analyses showed that 10 factors (male sex,age,BMI,FPG,hypertension,TG,TCh,HDL-C, LDL-C,and ALT abnormalities)were closely related to FLD. In heavy drinkers,obesity increased the risk of FLD by 23.78-fold(95%CI,10.22-55.33),but heavy drinking was only associated with a 2-fold(95%CI,1.50-2.66)increased risk in obese subjects.CONCLUSIONS:The prevalence of FLD among a health- checkup population in Chengdu,Southwest China was lower than the published for other areas of China.FLD in Chengdu adults was found to be closely associated with sex, age,BMI,and other metabolic syndrome features. | Li, Hong Wang, You-Juan Tan, Ke Zeng, Li Liu, Li Liu, Feng-Jun Zhou, Tao-You Chen, En-Qiang Tang, Hong | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，4： | 22 |
| 8 | Preparation, characterization and photocatalytic activities of boron- and cerium-codoped TiO_2显示文摘Boron-codoped 和 cerium-codoped TiO2 光催化剂用modifiedsol胶化反应过程被综合并且由X光检查衍射( XRD )描绘了,X光检查 photoelectronspectroscopy ( XPS ),粒子尺寸分发( PSD ),弥漫的反射系列(医生), andBrunauer-Emmett-Teller (赌注) .The photocatalytic 活动被监视结果显示出的染料酸红 B ( ARB ) .The 的 thedegradation 评估准备光催化剂是主要由 titania , ceria ,和硼 oxide.The 结构组成的 | WEI Chao-hai TANG Xin-hu LIANG Jie-rong TAN Shu-ying | 2007 | Journal of Environmental Sciences2007，19，1： | 22 |
| 9 | Hemiballism-hemichorea induced by ketotic hyperglycemia: case report with PET study and review of the literature显示文摘Hemiballism-hemichorea(HB-HC)is commonly used to describe the basal ganglion dysfunction in non-ketotic hyperglycemic elderly patients.Here we report two elderly female patients with acute onset of involuntary movements induced by hyperglycemia with positive urine ketones.We described the computed tomography and magnetic resonance imaging findings in these two patients,which is similar to that of non-ketotic hyperglycemic HB-HC patients.FDG-PET was performed and the glucose metabolism in the corresponding lesion in these two patients was contradictory with each other.We tried to clarify the underlying mechanisms of HB-HC and explain the contradictory neuroradiological findings in FDG-PET as being performed at different clinical stages. | Yuyan Tan Xiaoyu Xin Qin Xiao Shengdi Chen Li Cao Huidong Tang | 2014 | Translational Neurodegeneration2014，3，1： | 21 |
| 10 | The material and biological characteristics of osteoinductive calcium phosphate ceramics显示文摘The discovery of osteoinductivity of calcium phosphate(Ca-P)ceramics has set an enduring paradigm of conferring biological regenerative activity to materials with carefully designed structural characteristics.The unique phase composition and porous structural features of osteoinductive Ca-P ceramics allow it to interact with signaling molecules and extracellular matrices in the host system,creating a local environment conducive to new bone formation.Mounting evidence now indicate that the osteoinductive activity of Ca-P ceramics is linked to their physicochemical and three-dimensional structural properties.Inspired by this conceptual breakthrough,many laboratories have shown that other materials can be also enticed to join the rank of tissue-inducing biomaterials,and besides the bones,other tissues such as cartilage,nerves and blood vessels were also regenerated with the assistance of biomaterials.Here,we give a brief historical recount about the discovery of the osteoinductivity of Ca-P ceramics,summarize the underlying material factors and biological characteristics,and discuss the mechanism of osteoinduction concerning protein adsorption,and the interaction with different types of cells,and the involvement of the vascular and immune systems. | Zhurong Tang Xiangfeng Li Yanfei Tan Hongsong Fan Xingdong Zhang | 2018 | Regenerative Biomaterials2018，5，1： | 20 |
| 11 | Ophiopogonin D maintains Ca2+ homeostasis in rat cardiomyocytes in vitro by upregulating CYP2J3/ EETs and suppressing ER stress显示文摘目的: 在心肌层的 CYP2J3 使 arachidonic 酸产生代射变化到 4 regioisomeric epoxyeicosatrienoic 酸(EET ) ,它在老鼠心举办多样的生物活动。在我们检验了 CYP2J3 是否涉及 ophiopogonin D (OPD ) 的 cardioprotective 效果的这研究, steroidal glycoside 从中国植物根值 ophiopogonis.Methods 孤立: 老鼠 cardiomyoblast 房间线(H9c2 房间) 被测试。细胞内部的 Ca 2+ 集中([Ca 2+] i ) 用 Fluo-4/AM 被测量。调整钙的分子的表示并且嗯应力发信号分子与 qRT-PCR 和西方的污点分析被测量。房间 apoptosis 与 Hoechst 33258 染色和 TUNEL 试金被确定。14,15-DHET 的水平, 14,15-EET 的稳定的代谢物,与 ELISA.Results 被估计: 血管收缩素 II (10 −6 mol/L ) 显著地减少了调整钙的分子(SERCA2a, PLB, RyR2 和 FKBP12.6 ) 的表示,并且提高[Ca 2+] 在 H9c2 房间的 i 。而且,血管收缩素 II 显著地增加了表示嗯应力发信号分子(GRP78,砍, p-JNK 和劈开的 caspase-12 ) 并且嗯调停压力的 apoptosis。OPD (100, 250 和 500 nmol/L ) dose-dependently 在正常 H9c2 房间增加了 CYP2J3 表示和 14,15-DHET 层次。有 OPD 的 H9c2 房间的预告的处理压制了血管收缩素在 Ca 2+ 动态平衡的导致 II 的畸形,嗯压力回答和 apoptosis。CYP2J3 的 Overexpression 或外长的 14,15-EET 的增加也阻止了血管收缩素在 Ca 2+ 动态平衡的导致 II 的畸形,而有 CYP2J3 siRNA 的 transfection 减少了 Ca 2+ 动态平衡上的 OPD 的效果。而且,细胞内部的 Ca 2+ chelator BAPTA 压制了血管收缩素导致 II 嗯在 H9c2 cells.Conclusion 的压力回答和 apoptosis : OPD 是可以在与 Ca 2+ 动态平衡的骚乱有关的心血管的疾病的处理提供一个治疗学的好处的新奇 CYP2J3 inducer 并且嗯应力。 | Wen-ting YOU Tao ZHOU Zeng-chun MA Qian-de LIANG Cheng-rong XIAO Xiang-lin TANG Hong-ling TAN Bo-li ZHANG Yu-guang WANG Yue GAO | 2016 | Acta Pharmacologica Sinica2016，37，3： | 19 |
| 12 | Ibrolipim increases ABCA1/G1 expression by the LXRα signaling pathway in THP-1 macrophage- derived foam cells显示文摘 | Si-guo CHEN Ji XIAO Xie-hong LIU Mei-mei LIU Zhong-cheng MO Kai YIN Guo-jun ZHAO Jin JIANG Li-bao CUI Chun-zhi TAN Wei-dong YIN Chao-ke TANG | 2010 | Acta Pharmacologica Sinica2010，31，10： | 18 |
| 13 | Simvastatin inhibits apoptosis of endothelial cells induced by sepsis through upregulating the expression of Bcl-2 and downregulating Bax显示文摘BACKGROUND: Many studies have showed that apoptosis of endothelial cells plays a curial role in the progress of sepsis. But the role of simvastatin in apoptosis of endothelial cells induced by sepsis is not clear. The present study aimed to investigate the role of simvastatin in apoptosis of endothelial cells induced by sepsis and its mechanism.METHODS: Human umbilical vein endothelial cells(HUVECs) were randomly divided into three groups: control group, sepsis serum intervention group(sepsis group) and simvastatin+sepsis serum intervention group(simvastatin group). After 24-hour incubation with corresponding culture medium, the relative growth rate of HUVECS in different groups was detected by MTT assay; the apoptosis of HUVECs was detected by Hoechst33258 assay and fl ow cytometry; and the expression of the Bcl-2 and Bax genes of HUVECs was detected by PCR.RESULTS: Compared with the sepsis group, HUVECs in the simvastatin group had a higher relative growth rate. Apoptotic HUVECs decreased significantly in the simvastatin group in comparison with the sepsis group. Expression of the Bcl-2 gene in HUVECs decreased obviously, but the expression of the Bax gene increased obviously after 24-hour incubation with sepsis serum; however, the expression of the Bcl-2 and Bax genes was just the opposite in the simvastatin group.CONCLUSIONS: Our study suggests that simvastatin can inhibit apoptosis of endothelial cells induced by sepsis through upregulating the expression of Bcl-2 and downregulating Bax. It may be one of the mechanisms for simvastatin to treat sepsis. | Hui Fu Qiao-sheng Wang Qiong Luo Si Tan Hua Su Shi-lin Tang Zheng-liang Zhao Li-ping Huang | 2014 | World Journal of Emergency Medicine2014，5，4： | 18 |
| 14 | Cardioprotection of Shenfu preparata on cardiac myocytes through cytochrome P450 2J3显示文摘OBJECTIVE:To evaluate whether Shenfu injection(SFI)protects against cardiac myocyte injury induced by Fupian injection(FPI)in vitro.METHODS:H9c2 cells were separately treated with FPI,Renshen injection(RSI)and SFI.Cell viability,lactate dehydrogenase(LDH)release,spontaneous beating rate of primative cardical cells,caspase-3/7 activity,cell apoptosis,and cytochrome P450 2J3(CYP2J3)mRNA expression were analyzed.RESULTS:The viability of H9c2 cells treated with SFI(37 and 75 mg/mL)was significantly higher than that of H9c2 cells treated with FPI(25 and 50 mg/mL)(P<0.05,P<0.01,respectively).LDH activity of H9c2 cells treated with SFI(75 mg/mL)was significantly decreased(P<0.01)compared with that of H9c2 cells treated with FPI(50 mg/mL).SFI(150 mg/mL)significantly attenuated FPI(100 mg/mL)-induced spontaneous beating rate decrease in primary myocardial cells after4-hour treatment.Compared with FPI(12 and 25 mg/mL),SFI(18 and 37 mg/mL)treatment could effectively reverse the change of caspase-3/7 activity(P<0.01 and P<0.01,respectively).Compared with FPI(6 and 25 mg/mL),apoptotic cells decreased significantly(P<0.05,P<0.01,respectively)when H9c2 cells were incubated with SFI(9 and 37 mg/mL).The expression of CYP2J3 mRNA was down-regulated by FPI,while RSI and SFI could up-regulate the expression of CYP2J3(P<0.01),which suggested the potential mechanism of protection of RSI against cardiac myocyte damage induced by FPI treatment.CONCLUSION:These observations indicate that SFI has the potential to exert cardioprotective effects against FPI toxicity.The effect was possibly correlated with the activation of CYP2J3. | Yong Xiao Zeng-chun Ma Yu-guang Wang Hong-ling Tan Xiang-ling Tang Qian-de Liang Cheng-rong Xiao Yue Gao | 2013 | Journal of Integrative Medicine2013，11，5： | 17 |
| 15 | Limit analysis of extended reach drilling in South China Sea显示文摘Extended reach wells (ERWs), especially horizontal extended reach well with a high HD (horizontal displacement) to TVD (true vertical depth) ratio, represent a frontier technology and challenge the drilling limitations. Oil and gas reservoir in beaches or lakes and offshore can be effectively exploited by using extended reach drilling (ERD) technology. This paper focuses on the difficult technological problems encountered during exploiting the Liuhua 11-1 oil fi eld in the South China Sea, China. Emphasis is on investigating the key subjects including prediction and control of open hole limit extension in offshore ERD, prediction of casing wear and its prevention and torque reduction,φ244.5mm casing running with floating collars to control drag force, and steerable drilling modes. The basic concept of limit extension in ERD is presented and the prediction method for open hole limit extension is given in this paper. A set of advanced drilling mechanics and control technology has been established and its practical results are verifi ed by fi eld cases. All those efforts may be signifi cant for further investigating and practicing ERD limit theory and control technology in the future. | Gao Deli Tan Chengjin Tang Haixiong | 2009 | Petroleum Science2009，6，2： | 17 |
| 16 | Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines显示文摘Osteoporosis is a frequent complication of chronic inflammatory diseases and increases in the pro-inflammatory cytokines make an important contribution to bone loss by promoting bone resorption and impairing bone formation. Omentin-1 is a newly identified adipocytokine that has anti-inflammatory effects, but little is known about the role of omentin-1 in inflammatory osteoporosis. Here we generated global omentin-1 knockout(omentin-1^(-/-)) mice and demonstrated that depletion of omentin-1 induces inflammatory bone loss-like phenotypes in mice, as defined by abnormally elevated pro-inflammatory cytokines, increased osteoclast formation and bone tissue destruction, as well as impaired osteogenic activities. Using an inflammatory cell model induced by tumor necrosis factor-α(TNF-α), we determined that recombinant omentin-1 reduces the production of proinflammatory factors in the TNF-α-activated macrophages, and suppresses their anti-osteoblastic and pro-osteoclastic abilities. In the magnesium silicate-induced inflammatory osteoporosis mouse model, the systemic administration of adenoviral-delivered omentin-1 significantly protects from osteoporotic bone loss and inflammation. Our study suggests that omentin-1 can be used as a promising therapeutic agent for the prevention or treatment of inflammatory bone diseases by downregulating the proinflammatory cytokines. | Shan-Shan Rao Yin Hu Ping-Li Xie Jia Cao Zhen-Xing Wang Jiang-Hua Liu Hao Yin Jie Huang Yi-Juan Tan Juan Luo Ming-Jie Luo Si-Yuan Tang Tuan-Hui Chen Ling-Qing Yuan Er-Yuan Liao Ran Xu Zheng-Zhao Liu Chun-Yuan Chen Hui Xie | 2018 | Bone Research2018，6，2： | 16 |
| 17 | Footprint Mismatch of Cervical Disc Prostheses with Chinese Cervical Anatomic Dimensions显示文摘 | Liang Dong Ming-Sheng Tan Qin-Hua Yan Ping Yi Feng Yang Xiang-Sheng Tang Qing-Ying Hao | 2015 | Chinese Medical Journal2015，，2： | 15 |
| 18 | miR-374b-5p suppresses RECK expression and promotes gastric cancer cell invasion and metastasis显示文摘AIM:To profile expression of micro RNAs(mi RNAs)in gastric cancer cells and investigate the effect of mi R-374b-5p on gastric cancer cell invasion and metastasis.METHODS:An mi RNA microarray assay was performed to identify mi RNAs differentially expressed in gastric cancer cell lines(MGC-803 and SGC-7901)compared with a normal gastric epithelial cell line.Upregulation of mi R-374b-5p was newly identified and confirmed via quantitative real-time reverse transcriptionPCR(q RT-PCR).MGC-803 cells were transfected with a synthesized anti-mi R-374b-5p sequence or a control vector using Lipofectamine reagent,or treated with transfection reagent alone or phosphate-buffered saline as controls.Rate of transfection was verified after 48 h by q RT-PCR.Cells were then subjected to transwell migration,wound scratch and cell counting kit-8 assays.A bioinformatic analysis to identify mi R-374b-5p target genes was performed using mi Randa,Pic Tar and Target Scan software.A dual luciferase reporter assay was performed to evaluate the influence of mi R-374b-5p on target gene activation,and q RT-PCR and Western blot were used to evaluate the levels of target m RNA and protein following transfection with mi R-374b-5p antisense oligonucleotides.RESULTS:The microarray profiling revealed downregulation of 14(fold change<0.667;P<0.05)and upregulation of 12(fold change>1.50;P<0.05)mi RNAs in MGC-803 and SGC-7901 cells compared with GES-1 controls.The upregulation of mi R-374b-5p(fold change=1.75 and 1.64 in MGC-803 and SGC-7901,respectively;P<0.05)was confirmed by q RT-PCR.Compared with the control groups,the restoration of mi R-374b-5p expression with anti-mi R-374b-5p significantly suppressed the metastasis,invasion and proliferation of MGC-803 cells.The bioinformatic analysis predicted that the 3’untranslated region(UTR)of reversion-inducing cysteine-rich protein with Kazal motif(RECK)contains three mi R-374b-5p target sequences.RECK was verified as a target gene in a dual luciferase reporter assay showing that activation of RECK 3’UTR-pmir GLO was increased by co-transfection with mi R-374b-5p.Finally,transfection of mi R-374b-5p antisense oligonucleotides increased m RNA and protein levels of RECK in MGC-803cells(P<0.05).CONCLUSION:These findings indicate that upregulation of mi R-374b-5p contributes to gastric cancer cell metastasis and invasion through inhibition of RECK expression. | Juan Xie Zhi-Hui Tan Xia Tang Ming-Shu Mo Yan-Ping Liu Run-Liang Gan Yi Li Li Zhang Guo-Qing Li | 2014 | World Journal of Gastroenterology2014，20，46： | 15 |
| 19 | Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress显示文摘BACKGROUND Abdominal paracentesis drainage(APD)is a safe and effective strategy for severe acute pancreatitis(SAP)patients.However,the effects of APD treatment on SAPassociated cardiac injury remain unknown.AIM To investigate the protective effects of APD on SAP-associated cardiac injury and the underlying mechanisms.METHODS SAP was induced by 5%sodium taurocholate retrograde injection in Sprague-Dawley rats.APD was performed by inserting a drainage tube with a vacuum ball into the lower right abdomen of the rats immediately after SAP induction.Morphological staining,serum amylase and inflammatory mediators,serum and ascites high mobility group box(HMGB)1,cardiac-related enzymes indexes and cardiac function,oxidative stress markers and apoptosis and associated proteins were assessed in the myocardium in SAP rats.Nicotinamide adenine dinucleotide phosphate oxidase activity and mRNA and protein expression were also examined.RESULTS APD treatment improved cardiac morphological changes,inhibited cardiac dysfunction,decreased cardiac enzymes and reduced cardiomyocyte apoptosis,proapoptotic Bax and cleaved caspase-3 protein levels.APD significantly decreased serum levels of HMGB1,inhibited nicotinamide adenine dinucleotide phosphate oxidase expression and ultimately alleviated cardiac oxidative injury.Furthermore,the activation of cardiac nicotinamide adenine dinucleotide phosphate oxidase by pancreatitis-associated ascitic fluid intraperitoneal injection was effectively inhibited by adding anti-HMGB1 neutralizing antibody in rats with mild acute pancreatitis.CONCLUSION APD treatment could exert cardioprotective effects on SAP-associated cardiac injury through suppressing HMGB1-mediated oxidative stress,which may be a novel mechanism behind the effectiveness of APD on SAP. | Yi Wen Hong-Yu Sun Zhen Tan Ruo-Hong Liu Shang-Qing Huang Guang-Yu Chen Hao Qi Li-Jun Tang | 2020 | World Journal of Gastroenterology2020，26，1： | 14 |
| 20 | Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff.显示文摘Diversity and nitrogenase activity of endophytic diazotrophs colonized in the wild rice Oryza rufipogon Griff grown in Boluo, Huilai County in Guangdong Province and Lingshui County in Hainan Province were studied. Thirty-seven isolates obtained from Oryza rufipogon were identified as putative endophytic nitrogen-fixing bacteria by ARA (acetylene reduction assay) test and further confirmed by PCR amplification of nifH gene fragments. All obtained strains have ARA activity and the same sized nifH gene fragments. Above the similarity level of 80%, the obtained isolates were assigned as Group Ⅰ to Ⅷ by the clustering of IS-PCR fingerprints. The SDS-PAGE whole-cell protein patterns were similar to those of IS-PCR fingerprints. Components and contents of fatty acid methyl esters (FAMEs) were used to differentiate the representative strains (Ls13, Ls8, BL1, BL12, HL6, Ls4) from Group Ⅰ to Group Ⅵ. The six representative strains showed significant difference in contents and components of cellular fatty acid methyl ester. 16S rDNA sequencing analysis showed that strains of Group Ⅰ to Ⅶ were located in Enterobacteraceae (γ-proteobacteria). Strains of Group Ⅰ and Group II were closely related to Klebsiella sp.; Strain Ls8 of Group Ⅱ was a little far away from the genus of Pantoea (homology level 96% with Pantoea agglomerans), which may represent a new species or genus in Enterobacteraceae; Strains of Groups IV and V belonged to different Enterobacter sp.; Strain Ls4 and Ls 9 representing Group Ⅵ were close to Citrobacter amalonaticus with 98% sequence similarity; Strain Ls15 of Group Ⅶ showed 98% sequence identity with Pantoea sp.; Strains of Group Ⅷ were assigned to the genus Ideonella (β-proteobacteria). Based on the above results, endophytic diazotrophs isolated from O. rufipogon showed great diversity and some diazotrophs showed high nitrogenase activity with 42.52 μmol/mL·h C2H4. Inoculation to rice tests indicated that the isolated endophytic diazotrophs significantly promoted the rice growth. | TAN ZhiYuan PENG GuiXiang XU PeiZhi AI ShaoYing TANG ShuanHu ZHANG GuoXia ZENG FengYun | 2009 | Chinese Science Bulletin2009，54，16： | 14 |
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