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| 1 | Exosomes from Suxiao Jiuxin pill-treated cardiac mesenchymal stem cells decrease H3K27 demethylase UTX expression in mouse cardiomyocytes in vitro显示文摘Suxiao Jiuxin 药片(SJP ) 是为在中国的急性冠的症候群的处理的繁体中文药,它包含二个主要部件, tetramethylpyrazine (TMP ) 和龙脑(BOR ) 。因此远然而,在心脏的微型环境上位于 SJP 的有益的效果下面的分子的机制是未知的。心脏的间充质的干细胞(C-MSCs ) 通过 microvesicles (exosomes ) 的版本与 cardiomyocytes (厘米) 交流恢复心脏的动态平衡并且得到修理,部分地通过 epigenetic 规章的机制。在这研究,我们检验了 SJP 处理是否改变了 C-MSC-derived exosomes (SJP-Exos ) 引起 epigenetic 在接受者厘米的色彩的改变。从老鼠心孤立的 C-MSC 是有 SJP (SJP-Exos ) 的 pretreated, TMP (TMP-Exos ) 或 BOR (BOR-Exos ) 。然后, HL-1 房间,一根鼠标 cardiomyocyte 线,从控制与 exosomes 被对待 C-MSCs (Ctrl-Exos ) , SJP-Exos, TMP-Exos 或 BOR-Exos。有 SJP-Exos 的处理显著地增加了 histone 的蛋白质层次 3 离氨酸 27 trimethylation (H3K27me3 ) ,为心脏的 transcriptional 抑制的一个关键 epigenetic 染色质标记,在 HL-1 房间。为了推进,探索 SJP-Exo-mediated H3K27me3 upregulation 的机制,我们在对待 exosome 的 HL-1 房间估计了关键 histone methylases (EZH1, EZH2 和 EED ) 和 demethylases (JMJD3 和 UTX ) 的 mRNA 表示层次。有 SJP-Exo 的处理有选择地在接受者 HL-1 房间压制了 UTX 表示。而且, PCNA,房间复制的一个内长的标记,比在 Ctrl-Exo-treated HL-1 房间在 SJP-Exo-treated HL-1 房间是显著地更高的。这些结果证明 SJP-Exos 增加 cardiomyocyte 增长并且证明 SJP 能调制 C-MSC-derived exosomes 引起 epigenetic 染色质在接受者 cardiomyocytes 改变;因而, SJP-Exos 可能被用来支持 cardiomyocyte 增长。 | Xiao-fen RUAN Yongdun LI Cheng-wei JU Yan SHEN Wei LEI Can CHEN Yang LI Hong YU Yu-tao LIU II-man KIM Xiao-long WANG Neal L WEINTRAUB Yaoliang TANG | 2018 | Acta Pharmacologica Sinica2018，39，4： | 23 |
| 2 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 3 | Suxiao Jiuxin pill promotes exosome secretion from mouse cardiac mesenchymal stem cells in vitro显示文摘心脏的间充质的干细胞(C-MSCs ) 是在损害以后在心修理起一个作用的内长的心脏的 stromal 房间。C-MSC-derived exosomes (Exo ) 对尖锐心肌的 ischemia/reperfusion 导致的 apoptosis 显示出保护的效果。Suxiao Jiuxin 药片(SJP ) 是为尖锐心肌的局部缺血的处理在中国使用的一个繁体中文药(TCM ) 公式,它作为主要部件包含 tetramethylpyrazine (TMP ) 和龙脑(BOR ) 。在这研究,我们调查了 SJP 处理是否在 vitro 从 C-MSCs 影响了 exosome 版本。从老鼠准备的 C-MSCs 与 SJP (62.5 g/mL ) 被对待, TMP (25 g/mL ) 或 BOR (15 g/mL ) 。用 acetylcholinesterase 活动试金,我们发现 SJP 和 TMP 处理显著地与控制乙醇处理相比增加了 exosome 分泌物。中立 sphingomyelinase 2 (nSMase2 ) 小径在 exosome 形成是重要的并且包装。但是 nSMase2 mRNA 的既不水平也不蛋白质的水平改变了后面的 SJP, TMP 或 BOR 处理,建议那 SJP 经由一条 nSMase2 独立的小径刺激了 exosome 版本。Rab27a 和 Rab27b GTPases 控制了 exosome 分泌物小径的不同步骤。我们证明 SJP 处理显著地与控制处理相比增加了 Rab27a, SYTL4 (Rab27a 受动器) 和 Rab27b 的蛋白质层次。SJP 处理另外显著地 upregulated Rab27b 的 mRNA 水平,而非 Rab27a。而且, C-MSC-exosome 版本的导致 SJP 的增加被 Rab27b 禁止击倒,建议那 SJP 经由一条 GTPase 依赖的小径从 C-MSCs 支持 exosome 分泌物。这研究在 modulating 为 SJP 揭示新奇机制心脏的动态平衡。 | Xiao-fen RUAN Cheng-wei JU Yan SHEN Yu-tao LIU II-man KIM Hong YU Neal WEINTRAUB Xiao-long WANG Yaoliang TANG | 2018 | Acta Pharmacologica Sinica2018，39，4： | 19 |
| 4 | Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction 显示文摘 | Tang YaoLiang Zhao Qiang Qin Xinyu | 2005 | Ann Thorac Surg2005，80，1： | 1 |
| 5 | Extracellular vesicles as novel biomarkers and pharmaceutic targets of diseases显示文摘 | Yanfang CHEN Yaoliang TANG Guo-Chang FAN Dayue Darrel DUAN | 2018 | Acta Pharmacologica Sinica2018，39，4： | 1 |
| 6 | Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction 显示文摘 | Tang Yaoliang Zhao Qiang Qin Xinyu | 2005 | Ann Thorac Surg2005，80，1： | 1 |
| 7 | Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium显示文摘 | Tang Yaoliang Zhao Qiang Zhang YC | 2004 | Regulatory Peptides2004，117，1： | 1 |
| 8 | Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury显示文摘 | Lijuan Chen Yingjie Wang Yaohua Pan Lan Zhang Chengxing Shen Gangjian Qin Muhammad Ashraf Neal Weintraub Genshan Ma Yaoliang Tang | 2013 | Biochemical and Biophysical Research Communications2013，，3： | 1 |
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