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73篇 您的检索式:作者名="Xijun Li"
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1A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs显示文摘African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig industry.An efficacious vaccine is urgently required.Here,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted viruses.The virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV challenge.Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV.Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu 2020Science China(Life Sciences)2020,63,5:72
2Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020显示文摘African swine fever virus(ASFV)has been circulating in China for more than two years,and it is not clear whether the biological properties of the virus have changed.Here,we report on our surveillance of ASFVs in seven provinces of China,from June to December,2020.A total of 22 viruses were isolated and characterized as genotype II ASFVs,with mutations,deletions,insertions,or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018(HLJ/18),the earliest isolate in China.Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a nonhemadsorbing(non-HAD)phenotype.Four isolates were tested for virulence in pigs;two were found to be as highly lethal as HLJ/18.However,two non-HAD isolates showed lower virulence but were highly transmissible;infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease,whereas 103 TCID50 dose caused non-lethal,sub-acute or chronic disease,and persistent infection.The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control.Encheng Sun Zhenjiang Zhang Zilong Wang Xijun He Xianfeng Zhang Lulu Wang Wenqing Wang Lianyu Huang Fei Xi Haoyue Huangfu Ghebremedhin Tsegay Hong Huo Jianhong Sun Zhijun Tian Wei Xia Xuewu Yu Fang Li Renqiang Liu Yuntao Guan Dongming Zhao Zhigao Bu 2021Science China(Life Sciences)2021,64,5:46
3Temperature pattern dynamics in shocked porous materials显示文摘The physical fields in porous materials under strong shock wave reaction are very complicated. We simulate such systems using the grain contact material point method. The complex temperature fields in the material are treated with the morphological characterization. To compare the structures and evolution of characteristic regimes under various temperature thresholds, we introduce two concepts, structure similarity and process similarity. It is found that the temperature pattern dynamics may show high similarity under various conditions. Within the same material, the structures and evolution of high-temperature regimes may show high similarity if the shock strength and temperature threshold are chosen appropriately. For process similarity in materials with high porosity, the required temperature threshold increases parabolically with the impact velocity. When the porosity becomes lower, the increasing rate becomes higher. For process similarity in different materials, the required temperature threshold and the porosity follow a power-law relationship in some range.XU AiGuo*, ZHANG GuangCai, LI Hua, YING YangJun, YU XiJun & ZHU JianShi National Key Laboratory of Computational Physics, Institute of Applied Physics and Computational Mathematics, Beijing 100088, China 2010Science China(Physics,Mechanics & Astronomy)2010,53,8:7
4Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency syndrome显示文摘Sijunzi decoction (SJZD) is a Chinese classical formula to treat spleen qi deficiency syndrome (SQDS) and has been widely used for thousands of years.However,the quality control (QC) standards of SJZD are insufficient.Chinmedomics has been designed to discover and verify bioactive compounds of a variety of formularapidly.In this study,we used Chinmedomics to evaluate the SJZD's efficacy against SQDS to discover the potential quality-markers (q-markers) for QC.A total of 56 compounds in SJZD were characterized in vitro,and 23 compounds were discovered in vivo.A total of 58 biomarkers were related to SQDS,and SJZD can adjust a large proportion of marker metabolites to normal level and then regulate the metabolic profile to the health status.A total of 10 constituents were absorbed as effective ingredients that were associated with overall efficacy.We preliminarily determined malonyl-ginsenoside Rb2 and ginsenoside Ro as the q-markers of ginseng;dehydrotumulosic acid and dihydroxy lanostene-triene-21-acid as the q-markers of poria;glycyrrhizic acid,isoglabrolide,and glycyrrhetnic acid as the q-markers of licorice;and 2-atractylenolide as the q-marker of macrocephala.According to the discovery of the SJZD q-markers,we can establish the quality standard that is related to efficacy.Qiqi Zhao Xin Gao Guangli Yan Aihua Zhang Hui Sun Ying Han Wenxiu Li Liang Liu Xijun Wang 2020Frontiers of Medicine2020,14,3:7
5Massive cranium from Harbin in northeastern China establishes a new Middle Pleistocene human lineage显示文摘It has recently become clear that several human lineages coexisted with Homo sapiens during the late Middle and Late Pleistocene.Here,we report an archaic human fossil that throws new light on debates concerning the diversification of the Homo genus and the origin of H.sapiens.The fossil was recovered in Harbin city in northeastern China,with a minimum uranium-series age of 146 ka.This cranium is one of the best preserved Middle Pleistocene human fossils.Its massive size,with a large cranial capacity(1,420 mL)falling in the range of modern humans,is combined with a mosaic of primitive and derived characters.It differs from all the other named Homo species by presenting a combination of features,such as long and low cranial vault,a wide and low face,large and almost square orbits,gently curved but massively developed supraorbital torus,flat and low cheekbones with a shallow canine fossa,and a shallow palate with thick alveolar bone supporting very large molars.The excellent preservation of the Harbin cranium advances our understanding of several less-complete late Middle Pleistocene fossils from China,which have been interpreted as local evolutionary intermediates between the earlier species Homo erectus and later H.sapiens.Phylogenetic analyses based on parsimony criteria and Bayesian tip-dating suggest that the Harbin cranium and some other Middle Pleistocene human fossils from China,such as those from Dali and Xiahe,form a third East Asian lineage,which is a part of the sister group of the H.sapiens lineage.Our analyses of such morphologically distinctive archaic human lineages from Asia,Europe,and Africa suggest that the diversification of the Homo genus may have had a much deeper timescale than previously presumed.Sympatric isolation of small populations combined with stochastic long-distance dispersals is the best fitting biogeographical model for interpreting the evolution of the Homo genus.Xijun Ni Qiang Ji Wensheng Wu Qingfeng Shao Yannan Ji Chi Zhang Lei Liang Junyi Ge Zhen Guo Jinhua Li Qiang Li Rainer Grun Chris Stringer 2021The Innovation2021,2,3:6
6Atomic Fe-Zn dual-metal sites for high-efficiency pH-universal oxygen reduction catalysis显示文摘An effective electrocatalyst being highly active in all pH range for oxygen reduction reaction(ORR)is crucial for energy conversion and storage devices.However,most of the high-efficiency ORR catalysis was reported in alkaline conditions.Herein,we demonstrated the preparation of atomically dispersed Fe-Zn pairs anchored on porous N-doped carbon frameworks(Fe-Zn-SA/NC),which works efficiently as ORR catalyst in the whole pH range.It achieves high half-wave potentials of 0.78,0.85 and 0.72 V in 0.1 M HClO4,0.1 M KOH and 0.1 M phosphate buffer saline(PBS)solutions,respectively,as well as respectable stability.The performances are even comparable to Pt/C.Furthermore,when assembled into a Zn-air battery,the high power density of 167.2 mWcm−2 and 120 h durability reveal the feasibility of Fe-Zn-SA/NC in real energy-related devices.Theoretical calculations demonstrate that the superior catalytic activity of Fe-Zn-SA/NC can be contributed to the lower energy barriers of ORR at the Fe-Zn-N6 centers.This work demonstrates the potential of Fe-Zn pairs as alternatives to the Pt catalysts for efficient catalytic ORR and provides new insights of dual-atom catalysts for other energy conversion related catalytic reactions.Jie Xu Shuhua Lai Defeng Qi Min Hu Xianyun Peng Yifan Liu Wei Liu Guangzhi Hu Heng Xu Fan Li Chao Li Jia He Longchao Zhuo Jiaqiang Sun Yuan Qiu Shusheng Zhang Jun Luo Xijun Liu 2021Nano Research2021,14,5:6
7A conservative local discontinuous Galerkin method for the solution of nonlinear Schrdinger equation in two dimensions显示文摘In this study, we present a conservative local discontinuous Galerkin(LDG) method for numerically solving the two-dimensional nonlinear Schrdinger(NLS) equation. The NLS equation is rewritten as a firstorder system and then we construct the LDG formulation with appropriate numerical flux. The mass and energy conserving laws for the semi-discrete formulation can be proved based on different choices of numerical fluxes such as the central, alternative and upwind-based flux. We will propose two kinds of time discretization methods for the semi-discrete formulation. One is based on Crank-Nicolson method and can be proved to preserve the discrete mass and energy conservation. The other one is Krylov implicit integration factor(IIF) method which demands much less computational effort. Various numerical experiments are presented to demonstrate the conservation law of mass and energy, the optimal rates of convergence, and the blow-up phenomenon.ZHANG RongPei YU XiJun LI MingJun LI XiangGui 2017Science China Mathematics2017,60,12:5
8Traffic Characteristics Based Dynamic Radio Resource Management in Heterogeneous Wireless Networks显示文摘The traffic with tidal phenomenon in Heterogeneous Wireless Networks(HWNs)has radically increased the complexity of radio resource management and its performance analysis.In this paper,a Simplified Dynamic Hierarchy Resource Management(SDHRM)algorithm exploiting the resources dynamically and intelligently is proposed with the consideration of tidal traffic.In network-level resource allocation,the proposed algorithm first adopts wavelet neural network to forecast the traffic of each sub-area and then allocates the resources to those sub-areas to maximise the network utility.In connection-level network selection,based on the above resource allocation and the pre-defined QoS requirement,three typical network selection policies are provided to assign traffic flow to the most appropriate network.Furthermore,based on multidimensional Markov model,we analyse the performance of SDHRM in HWNs with heavy tailed traffic.Numerical results show that our theoretical values coincide with the simulation results and the SDHRM can improve the resource utilization.WEN Juan SHENG Min ZHANG Yan WANG Xijun LI Yuzhou 2014China Communications2014,11,1:4
9m6A RNA methylation-mediated HNF3γreduction renders hepatocellular carcinoma dedifferentiation and sorafenib resistance显示文摘Hepatocyte nuclear factor 3γ(HNF3γ)is a hepatocyte nuclear factor,but its role and clinical significance in hepatocellular carcinoma(HCC)remain unclear.Herein,we report that HNF3γexpression is downregulated in patient HCC and inversely correlated with HCC malignancy and patient survival.Moreover,our data suggested that the HNF3γreduction in HCC could be mediated by METTL14-dependent m6A methylation of HNF3γmRNA.HNF3γexpression was increased during hepatic differentiation and decreased in dedifferentiated HCC cells.Interestingly,HNF3γdelivery promoted differentiation of not only HCC cells but also liver CSCs,which led to suppression of HCC growth.Mechanistic analysis suggested an HNF3γ-centered regulatory network that includes essential liver differentiation-associated transcription factors and functional molecules,which could synergistically facilitate HCC cell differentiation.More importantly,enforced HNF3γexpression sensitized HCC cells to sorafenib-induced growth inhibition and cell apoptosis through transactivation of OATP1B1 and OATP1B3 expression,which are major membrane transporters for sorafenib uptake.Clinical investigation showed that patient-derived HCC xenografts with high HNF3γexpression exhibited a sorafenib response and patients with high HCC HNF3γlevels benefited from sorafenib therapy.Together,these results suggest that HNF3γplays an essential role in HCC differentiation and may serve as a therapeutic target and predictor of sorafenib benefit in patients.Tengfei Zhou Shichao Li Daimin Xiang Junyu Liu Wen Sun Xiuliang Cui Beifang Ning Xiao Li Zhuo Cheng Weiqi Jiang Cheng Zhang Xijun Liang Liang Li Xin Cheng Liu Hui Hongyang Wang Jin Ding 2020Signal Transduction and Targeted Therapy2020,5,1:4
10Geochemical provenancing and direct dating of the Harbin archaic human cranium显示文摘As one of the most complete archaic human fossils,the Harbin cranium provides critical evidence for studying the diversification of the Homo genus and the origin of Homo sapiens.However,the unsystematic recovery of this cranium and a long and confused history since the discovery impede its accurate dating.Here,we carried out a series of geochemical analyses,including non-destructive X-ray fluorescence(XRF),rare earth elements(REE),and the Sr isotopes,to test the reported provenance of the Harbin cranium and get better stratigraphic constraints.The results show that the Harbin cranium has very similar XRF element distribution patterns,REE concentration patterns,and Sr isotopic compositions to those of the Middle Pleistocene-Holocene mammalian and human fossils recently recovered from the Harbin area.The sediments adhered in the nasal cavity of the Harbin cranium have a 87Sr/86Sr ratio of 0.711898,falling in the variation range measured in a core drilled near the Dongjiang Bridge,where the cranium was discovered during its reconstruction.The regional stratigraphic correlations indicate that the Harbin cranium was probably from the upper part of the Upper Huangshan Formation of the Harbin area,which has an optically stimulated luminescence dating constraint between 138 and 309 ka.U-series disequilibrium dating(n=10)directly on the cranium suggests that the cranium is older than 146 ka.The multiple lines of evidence from our experiments consistently support the theory that the Harbin cranium is from the late Middle Pleistocene of the Harbin area.Our study also shows that geochemical approaches can provide reliable evidence for locating and dating unsystematically recovered human fossils,and potentially can be applied to other human fossils without clear provenance and stratigraphy records.Qingfeng Shao Junyi Ge Qiang Ji Jinhua Li Wensheng Wu Yannan Ji Tao Zhan Chi Zhang Qiang Li Rainer Grun Chris Stringer Xijun Ni 2021The Innovation2021,2,3:3
11Common-Ion Effect Triggered Highly Sustained Seawater Electrolysis with Additional NaCl Production显示文摘Developing efficient seawater-electrolysis system for mass production of hydrogen is highly desirable due to the abundance of seawater.However,continuous electrolysis with seawater feeding boosts the concentration of sodium chloride in the electrolyzer,leading to severe electrode corrosion and chlorine evolution.Herein,the common-ion effect was utilized into the electrolyzer to depress the solubility of NaCl.Specifically,utilization of 6M NaOH halved the solubility of NaCl in the electrolyte,affording efficient,durable,and sustained seawater electrolysis in NaCl-saturated electrolytes with triple production of H_(2),O_(2),and crystalline NaCl.Ternary NiCoFe phosphide was employed as a bifunctional anode and cathode in simulative and Ca/Mg-free seawater-electrolysis systems,which could stably work under 500 mA/cm^(2) for over 100 h.We attribute the high stability to the increased Na^(+)concentration,which reduces the concentration of dissolved Cl-in the electrolyte according to the common-ion effect,resulting in crystallization of NaCl,eliminated anode corrosion,and chlorine oxidation during continuous supplementation of Ca/Mg-free seawater to the electrolysis system.Pengsong Li Shiyuan Wang Imran Ahmed Samo Xingheng Zhang Zhaolei Wang Cheng Wang Yang Li Yiyun Du Yang Zhong Congtian Cheng Wenwen Xu Xijun Liu Yun Kuang Zhiyi Lu Xiaoming Sun 2020Research2020,,1:3
12A numerical study of passenger side airbag deployment based on arbitrary Lagrangian-eulerian method显示文摘The passenger side airbags(PAB)are usually larger than the driver airbags.Therefore,the inflator of PAB is more powerful with high mass rate.In this paper,an Arbitrary Lagrangian-Eulerian(ALE)method based computational method is developed to simulate the deployment of a PAB.The tank test is used to test the property of the inflator.Through comparison of numerical and experimental results,the ALE method is validated.Based on a failed airbag test,a smaller sub-airbag is placed inside PAB to disperse the gas flow to directions which are less damaging.By applying dynamic relaxation,the initial mesh corresponding to the experimental terms is obtained.The results indicate that the interior pressure and impact force coincide with the test data,and the method in this paper is capable of capturing airbag deploying process of the PAB module accurately.WANG XiJun ZONG Zhi ZHAO Yong ZOU Li 2015Science China(Technological Sciences)2015,58,3:3
13Two-in-one solution using insect wings to produce graphene-graphite films for efficient electrocatalysis显示文摘Natural organisms contain rich elements and naturally optimized smart structures,both of which have inspired various innovative concepts and desig ns in human society.In particular,several natural organisms have been used as element sources to synthesize low-cost and environmentally friendly electrocatalysts for the oxygen reduction reaction(ORR)in fuel cells and metal-air batteries,which are clean energy devices.However,to date,no naturally optimized smart structures have been employed in the synthesis of ORR catalysts,including graphene-based materials.Here,we demonstrate a novel strategy to synthesize graphene-graphite films(GGFs)by heating butterfly wings coated with FeCI3 in N2,in which the full power of natural organisms is utilized.The wings work not only as an element source for GGF generation but also as a porous supporting structure for effective nitrogen doping,two-dimensional spreading,and double-face exposure of the GGFs.These GGFs exhibit a half-wave potential of 0.942 V and a H2O2 yield of<0.07%for ORR electrocatalysis;these values are comparable to those for the best commercial Pt/C and all previously reported ORR catalysts in alkaline media.This two-in-one strategy is also successful with cicada and dragonfly wings,indicating that it is a universal,green,and cost-effective method for developing high-performance graphene-based materials.Huaiyu Li Lihan Zhang Long Li Chaowen Wu Yajiao Huo Ying Chen Xijun Liu Xiaoxing Ke Jun Luo Gustaaf Van Tendeloo 2019Nano Research2019,12,1:3
14H7N9 virus infection triggers lethal cytokine storm by activating gasdermin E-mediated pyroptosis of lung alveolar epithelial cells显示文摘The H7N9 influenza virus emerged in China in 2013,causing more than 1560 human infections,39% of which were fatal.A ‘cytokine storm’ in the lungs of H7N9 patients has been linked to a poor prognosis and death;however,the underlying mechanism that triggers the cytokine storm is unknown.Here,we found that efficient replication of the H7N9 virus in mouse lungs activates gasdermin E(GSDME)-mediated pyroptosis in alveolar epithelial cells,and that the released cytosolic contents then trigger a cytokine storm.Knockout of Gsdme switched the manner of death of A549 and human primary alveolar epithelial cells from pyroptosis to apoptosis upon H7N9 virus infection,and Gsdme knockout mice survived H7N9 virus lethal infection.Our findings reveal that GSDME activation is a key and unique mechanism for the pulmonary cytokine storm and lethal outcome of H7N9 virus infection and thus opens a new door for the development of antivirals against the H7N9 virus.Xiaopeng Wan Jiqing Li Yupeng Wang Xiaofei Yu Xijun He Jianzhong Shi Guohua Deng Xianying Zeng Guobin Tian Yanbing Li Yongping Jiang Yuntao Guan Chengjun Li Feng Shao Hualan Chen 2022National Science Review2022,9,1:3
15Identification of New Resistance Loci Against Sheath Blight Disease in Rice Through Genome-Wide Association Study显示文摘Sheath blight(SB) caused by the soil borne pathogen Rhizoctonia solani is one of the most serious global rice diseases. Breeding resistant cultivar is the most economical and effective strategy to control the disease. However, no rice varieties are completely resistant to SB, and only a few reliable quantitative trait loci(QTLs) linked with SB resistance have been identified to date. In this study, we conducted a genome-wide association study(GWAS) of SB resistance using 299 varieties from the rice diversity panel 1(RDP1) that were genotyped using 44 000 high-density single nucleotide polymorphism(SNP) markers. Through artificial inoculation, we found that only 36.5% of the tested varieties displayed resistance or moderate resistance to SB. In particular, the aromatic and aus sub-populations displayed higher SB resistance than the tropical japonica(TRJ), indica and temperate japonica sub-populations. Seven varieties showed similar resistance levels to the resistant control YSBR1. GWAS identified at least 11 SNP loci significantly associated with SB resistance in the three independent trials, leading to the identification of two reliable QTLs, qSB-3 and qSB-6, on chromosomes 3 and 6. Using favorable alleles or haplotypes of significantly associated SNP loci, we estimated that both QTLs had obvious effects on reducing SB disease severity and can be used for enhancing SB resistance, especially in improving SB resistance of TRJ sub-population rice varieties. These results provided important information and genetic materials for developing SB resistant varieties through breeding.CHEN Zongxiang FENG Zhiming KANG Houxiang ZHAO Jianhua CHEN Tianxiao LI Qianqian GONG Hongbing ZHANG Yafang CHEN Xijun PAN Xuebiao LIU Wende WANG Guoliang ZUO Shimin 2019Rice science2019,26,1:3
16Novel P22-monoclonal antibody based blocking ELISA for the detection of African swine fever virus antibodies in serum显示文摘African swine fever(ASF)is a highly infectious,transboundary viral disease of domestic and wild pigs,and is currently the most serious threat to world swine production,resulting in significant economic loss.In the absence of vaccines and treatments,the control of the disease entirely depends on accurate and early diagnosis accompanied by the culling of infected pigs.Thus,a highly specific and sensitive diagnostic assay is required during an outbreak and surveillance of the disease.In this study,a highly sensitive,specific,rapid and repeatable P22-monoclonal antibody-based blocking enzyme-linked immunosorbent assay(bELISA)assay was developed for the detection of antibodies against genotype I and II African swine fever viruses(ASFVs).A total of 806 pig serum samples were tested to evaluate the performance of the diagnostic assay.To determine the PI(percent Inhibition)cut-off value,receiver-operating characteristic(ROC)analysis was applied.According to the ROC analysis of the data,98.10%specificity and 100%sensitivity were recorded when the threshold cut-off value of PI was established at 47%.In addition,the assay was able to detect ASFV antibodies as early as 9 days post-infection when serum samples from experimentally infected pigs were used.Taking all together,the results of the present study indicated that the P22-mAb based bELISA assay can be used for rapid and accurate detection of antibodies against ASFV,which could play a valuable role in the containment and prevention of ASFV as an alternative to other serological diagnostic methods.Also,this study will assist researchers to further investigate the immunogenic importance of P22 protein in ASFV infection.Ghebremedhin Tsegay Weldu Tesfagaber Yuanmao Zhu Xijun He Wan Wang Zhenjiang Zhang Encheng Sun Jinya Zhang Yuntao Guan Fang Li Renqiang Liu Zhigao Bu Dongming Zhao 2022Biosafety and Health2022,4,4:2
17Chemically - modified activated carbon with ethylenediamine for selective solid -phase extraction and preconcentration of metal ions显示文摘LI Zhenhua CHANG Xijun ZOU Xiaojun ZHU Xiangbing NIE Rong HU Zheng LIRuijun 2009Anal Chim Acta2009,632,2:1
18Samarium (Ⅲ) adsorption on bentonite modified with N - (2 - hydroxyethyl ) ethyl- enediamine 显示文摘LI DANDAN CHANG XIJUN HU ZHENG 2011Talanta2011,83,:1
19Adsorption of chromium(Ⅲ):mercury(Ⅱ)and lead(Ⅱ)ions onto 4-aminoantipyrine immobilized bentonite显示文摘Wang Qihui Chang Xijun Li Dandan 0,,2:1
20H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che 2013Science2013,,6144:1
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