|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Puerarin reduces apoptosis in rat hippocampal neurons cultured in high glucose medium by modulating the p38 mitogen activated protein kinase and c-Jun N-terminal kinase signaling pathways显示文摘OBJECTIVE: To investigate the neuroprotective effect of puerarin on rat hippocampal neurons cultured in high glucose medium, and to examine the role of the p38 mitogen activated protein kinase(p38 MAPK) and c-Jun N-terminal kinase(JNK) signaling pathways in this effect.METHODS: Primary cultures of hippocampal neurons were prepared from newborn Sprague Dawley rats. Neuron-specific enolase immunocytochemistry was used to identify neurons. The neurons were cultured with normal medium(control group) or with high-glucose medium(high-glucose group),and puerarin(puerarin group), a p38 MAPK inhibitor(SB239063; p38 MAPK inhibitor group) or a JNK inhibitor(SP600125; JNK inhibitor group) were added. After 72 h of treatment, terminal deoxynucleotidyl transferase-mediated d UTP nick end labeling assay was performed to detect apoptosis, and western blotting was used to assess protein levels of p-p38, p38, p-JNK and JNK.RESULTS: In the high-glucose group, the neuronal apoptosis rate and the p-p38/p38 and p-JNK/JNK ratios were higher than in the control group. The p38 MAPK and JNK inhibitors prevented this increase in the apoptosis rate. The apoptosis rates in the puerarin group, the p38 MAPK inhibitor group and the JNK inhibitor group were significantly decreased compared with the high-glucose group.Moreover, protein levels of p-p38 and p-JNK were significantly reduced, and the p-p38/p38 and p-JNK/JNK ratios were decreased in the puerarin group compared with the high-glucose group. In addition, compared with the high-glucose group,p-p38 levels and the p-p38/p38 ratio were reduced in the p38 MAPK inhibitor group, and p-JNK levels and the p-JNK/JNK ratio were decreased in the JNK inhibitor group.CONCLUSION: Puerarin attenuates neuronal apoptosis induced by high glucose by reducing the phosphorylation of p38 and JNK. | Xu Xiaohan Wang Jingbo Zhang Hong Tian Guoqing Liu Yuqin | 2016 | Journal of Traditional Chinese Medicine2016，36，1： | 13 |
| 2 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 3 | Methionine deficiency and its hydroxy analogue influence chicken intestinal 3-dimensional organoid development显示文摘Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal regeneration.The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal development.Here,we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore the influence of methionine deficiency and MHA on intestinal organoid development.The results showed that 125-mm cell strainer exhibited the highest efficiency for chicken embryo crypt harvesting.We found that transforming growth factor-b inhibitor(A8301)supplementation promoted enterocyte differentiation at the expense of the proliferation of intestinal stem cells(ISC).The mitogen-activated protein kinase p38 inhibitor(SB202190)promoted intestinal organoid formation and enterocyte differentiation but suppressed the differentiation of enteroendocrine cells,goblet cells and Paneth cells.However,the suppression of enteroendocrine cell and Paneth cell differentiation by SB202190 was alleviated at the presence of A8301.The glycogen synthase kinase 3 inhibitor(CHIR99021),valproic acid(VPA)alone and their combination promoted chicken intestinal organoid formation and enterocyte differentiation at the expense of the expression of Paneth cells and goblet cells.Chicken serum significantly improved organoid formation,especially in the presence of A8301,SB202190,CHIR99021,and VPA,but inhibited the differentiation of Paneth cells and enteroendocrine cells.Chicken serum at a concentration of 0.25%meets the requirement of chicken intestinal organoid development,and the beneficial effect of chicken serum on chicken intestinal organoid culture could not be replaced by fetal bovine serum and insulin-like growth factor-1.Moreover,commercial mouse organoid culture medium supplemented with A8301,SB202190,CHIR99021,VPA,and chicken serum promotes chicken organoid budding.Based on the chicken intestinal organoid model,we found that methionine deficiency mimicked by cycloleucine suppressed organoid formation and organoid size,and this effect was reinforced with increased cycloleucine concentrations.Methionine hydroxy analogue promoted regeneration of ISC but decreased cell differentiation compared with the results obtained with L-methionine.In conclusion,our results provide a potentially excellent guideline for chicken intestinal organoid culture and insights into methionine function in crypt development. | Youli Wang Qihang Hou Yuqin Wu Yanwei Xu Yan Liu Jing Chen Lingling Xu Yuming Guo Shuai Gao Jianmin Yuan | 2022 | Animal Nutrition2022，，1： | 4 |
| 4 | Distribution of elements extracted from symptom patterns and characteristics of polysomnograph of common symptom patterns of insomnia with Traditional Chinese Medicine显示文摘OBJECTIVE: To analyze the distribution and combined regulation of elements of symptom patterns in the diagnosis of insomnia with Traditional Chinese Medicine(TCM).METHODS: The samples were collected from the patients, diagnosed with insomnia, of Henan Province Hospital of TCM between June 2011 and September 2013. The symptom patterns in insomnia were extracted. Next, symptom differentiation,characteristics of polysomnography(PSG), distribution and combined regulation of these symptom patterns were conducted by tests.RESULTS: In total, 286 eligible patients were recruited. The main locations of the disease symptom elements were the brain and heart, and the main characteristics of the disease symptom elements were phlegm-heat, Yin-deficiency and Qi-stagnation. The elements from two or three symptom patterns were commonly manifested in patients with insomnia, especially from three symptom patterns. We also found that all TCM symptom patterns had an effect on polysomnographic indicators in PSG tests.CONCLUSION: The elements of symptom patterns in insomnia were identified as mainly fire-heat and phlegm-heat. The most common patterns of excess were pathogenic fire derived from stagnation of liver-Qi, and mental disturbance due to phlegm-heat,while the most common patterns of deficiency in both the heart and the spleen. There are many differences in PSG indicators of different syndrome patterns of insomnia. | Cui Yinglin Zheng Weifeng Xu Li Meng Yi Wang Yuqin Chang Xuehui | 2016 | Journal of Traditional Chinese Medicine2016，36，5： | 4 |
| 5 | Transforming Co3O4 nanosheets into porous N-doped CoxOy nanosheets with oxygen vacancies for the oxygen evolution reaction显示文摘Cobalt oxides have been widely investigated as promising replacements for noble metal-based catalysts for oxygen evolution reaction(OER). Herein, we, for the first time, have obtained porous CoxOy nanosheets with N-doping and oxygen vacancies by etching Co3O4 nanosheets with NH3 plasma. Comparing with the pristine Co3O4 nanosheets(1.79 V), the porous CoxOy nanosheets with N-doping and oxygen vacancies have a much lower potential of 1.51 V versus RHE to reach the current density of 10 mA cm-2. The obtained sample has a lower Tafel slope of 68 m V dec-1 than the pristine Co3O4 nanosheets(234 mV dec-1).The disclosed Co^2+, which is responsible for the formation of active sites(CoOOH), N-doping and oxygen vacancies, gives rise to better performance of OER. | Lei Xu Yuqin Zou Zhaohui Xiao Shuangyin Wang | 2019 | Journal of Energy Chemistry2019，28，8： | 3 |
| 6 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 7 | Micro-spherical ZnSnO3 material prepared by microwave-assisted method and its ethanol sensing properties显示文摘Monodispersed ZnSnO3 microspheres are successfully prepared via a facile microwave-assisted method together with subsequently calcination treatment.Powder X-ray diffraction(PXRD)results indicate that the structure of the products shifted from crystalline to amorphous under high-tempe rature treatments.Field emission scanning electron microscope(FESEM)and the transmission electron microscope(TEM)observations demonstrate that the as-obtained products are composed of uniform microspheres with rough surfaces and the mean diameter is measured as-700 nm.Moreover,the morphology of ZnSnO3 microspheres can be well controlled by adjusting the ratio of Zn2+and Sn4+.The gas sensing properties of ZnSnO3 microspheres with different ratios of Zn2+/Sn4+are investigated.Our results indicate that the ZnSnO3 microspheres exhibit good selectivity and high sensitivity towards ethanol at the optimum working temperature of 230℃.When the sensor is exposed 50 ppm ethanol,the value of response is 47 and the response/recovery times are 11 s and 12 s,respectively. | Dan Zhang Yuqin Zhang Yu Fan Na Luo Zhixuan Cheng Jiaqiang Xu | 2020 | Chinese Chemical Letters2020，31，8： | 3 |
| 8 | Hybrid dual-channel phototransistor based on ID t-Se and 2D ReS2 mixed-dimensional heterostructures显示文摘The com bination of mixed-dimensional semiconducti ng materials can provide additional freedom to construct integrated n anoscale electronic and optoelectronic devices with diverse functionalities. In this work, we report a high-performanee dual-channel phototransistor based on one-dimensional (1D)/two-dimensional (2D) trigonal selenium (t-Se)/ReS2 heterostructures grown by chemical vapor deposition. The injection and separati on efficie ncy of photoge nerated electro n-hole pairs can be greatly improved due to the high-quality in terfacial con tact betwee n t-Se nano belts and ReS2 films. Compared with bare ReS2 film devices, the dual-cha nnel phototra nsistor based on t-Se/ReS2 heterostructure exhibits considerable enhancement with the responsivity (R) and detectivity (D^*) up to 98 A·W^-1 and 6 x 10^10 Jones at 400 nm illumination with an in tensity of 1.7 mW·cm^-2, respectively. Besides, the respo nse time can also be reduced by three times of magnitude to less than 50 ms due to the type-11 band alignment at the in terface. This study opens up a promising ave nue for high-performa nee photodetectors by constructing mixed-dimensional heterostructures. | Jingkai Qin Hang Yan Gang Qiu Mengwei Si Peng Miao Yuqin Duan Wenzhu Shao Liang Zhen Chengyan Xu Peide D Ye | 2019 | Nano Research2019，12，3： | 2 |
| 9 | A review on COVID-19 transmission,epidemiological features,prevention and vaccination显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused hundreds of millions of infections and millions of deaths over past two years.Currently,many countries have still not been able to take the pandemic under control.In this review,we systematically summarized what we have done to mitigate the COVID-19 pandemic,from the perspectives of virus transmission,public health control measures,to the development and vaccination of COVID-19 vaccines.As a virus most likely coming from bats,the SARS-CoV-2 may transmit among people via airborne,faecal-oral,vertical or foodborne routes.Ourmeta-analysis suggested that the R0 of COVID-19 was 2.9(95%CI:2.7–3.1),and the estimates in Africa and Europe could be higher.The median Rt could decrease by 23–96%following the nonpharmacological interventions,including lockdown,isolation,social distance,and face mask,etc.Comprehensive intervention and lockdown were the most effective measures to control the pandemic.According to the pooled R0 in our meta-analysis,there should be at least 93.3%(95%CI:89.9–96.2%)people being vaccinated around the world.Limited amount of vaccines and the inequity issues in vaccine allocation call for more international cooperation to achieve the antiepidemic goals and vaccination fairness. | Yuqin Zhang Gonghua Wu Shirui Chen Xu Ju Wumitijiang Yimaer Wangjian Zhang Shao Lin Yuantao Hao Jing Gu Jinghua Li | 2022 | Medical Review2022，2，1： | 2 |
| 10 | Sulfonic groups functionalized Zr-metal organic framework for highly catalytic transfer hydrogenation of furfural to furfuryl alcohol显示文摘The highly selective catalytic transfer hydrogenation(CTH)of furfural(FF)to furfuryl alcohol(FOL)is a significant route of biomass valorization.Herein,a series microporous Zr-metal organic framework(ZrMOF)functionalized by sulfonic groups are prepared.Based on the comprehensive structural characterizations by means of X-ray diffraction(XRD),scanning electron microscopy(SEM),transmission electron microscopy(TEM),N2 physisorption,Thermogravimetric(TG)and Fourier transformed infrared spectroscopy(FTIR),we find that sulfonic acid(–SO_(3)H)functional groups are tethered on the UIO-66 without affecting the structure of the framework.Systematic characterizations(NH_(3)-TPD,CO_(2)-TPD,and in-situ FTIR)demonstrate that modifying of sulfonic groups on UIO-66 results in the formation of stronger Lewis acidic-basic and Brnsted acidis sites.The cooperative role of the versatile Lewis acidic-basic and Brnsted acidic sites in 60%mol fraction of sulfonic acid-containing UIO-66(UIO-S_(0.6))retain high surface area and exhibit excellent catalytic performance of 94.7%FOL yield and 16.9 h^(-1).turnover number(TOF)under mild conditions.Kinetic experiments reveal that the activation energy of the CTH of furfural(FF)over UIO-S_(0.6) catalyst is as low as 50.8 k J mol^(-1).Besides,the hydrogen transfer mechanism is investigated through isotope labeling experiments,exhibiting that theβ-H in isopropanol is transferred to the a-C of FF by forming six-membered intermediates on the Lewis acidic-basic and Brnsted acidic sites of the UIO-S_(0.6),which is the rate-determining step in the formation of FOL. | Jingcheng Wu Dong Liang Xiangbo Song Tingsen Liu Tianyi Xu Shuangyin Wang Yuqin Zou | 2022 | Journal of Energy Chemistry2022，31，8： | 2 |
| 11 | Extraction of Lepidium apetalum Seed Oil Using Supercritical Carbon Dioxide and Anti-Oxidant Activity of the Extracted Oil显示文摘 | Wei Xu Kedan Chu Huang Li Lidian Chen Yuqin Zhang Xuchong Tang | | 0，，12： | 1 |
| 12 | The roles of ADIPOQ genetic variations in cancer risk: evidence from published studies显示文摘 | Yeqiong Xu Bangshun He Yuqin Pan Ling Gu Zhenlin Nie Liping Chen Rui Li Tianyi Gao Shukui Wang | 2013 | Molecular Biology Reports2013，，2： | 1 |
| 13 | Effects of annealing treatment and pH on preparation of citrate-stabilized PtRu/C catalyst 显示文摘 | XU Yuqin XIE Xiaofeng GUO Jianwei | 2006 | J Power Sources2006，162，： | 1 |
| 14 | Nano-effects quantum-like properties in electrospun nanofibers显示文摘 | He Jihuan Wan Yuqin Xu Lan | 2006 | Chaos Solitons & Fractals2006，33，： | 1 |
| 15 | Extraction of Lepidium apet alum Seed Oil Using Supercritical Carbon Dioxide and Anti-Oxidant Activity of the Extracted Oil显示文摘 | Wei Xu Kedan Chu Huang Li Lidian Chen Yuqin Zhang Xuchong Tang | 2011 | Molecules2011，16，10： | 1 |
| 16 | Research on Optimization of Recloser Placement of DG-enhanced Distribution Networks显示文摘 | ZHANG Li XU Yuqin WANG Zengping | | 0，，0885： | 1 |
| 17 | Micro Retail Enterprise in China: Healthier and More Mature显示文摘 | Walter Huber Yuqin Luan Yuning Ji Xinyue Xu Ziheng Xu | 2017 | Journal of US-China Public Administration2017，14，6： | 1 |
| 18 | A specific oligodeoxynucleotide promotes the differentiation of osteoblasts via ERK and p38 MAPK pathways 显示文摘 | Xu Hou Yuqin Shen Chao Zhang | 2012 | Int J Mol Sci2012，13，7： | 1 |
| 19 | Effect and mechanism of CyPA in radiosensitization of lung adenocarcinoma using CRISPR/Cas9 technology显示文摘Objective:To explore the role of cyclophilin A(CyPA)in sensitization of lung adenocarcinoma to radiotherapy using CRISPR/Cas9 technology.Methods:A CyPA knockout human lung adenocarcinoma cell line H1975 was established by CRISPR/Cas9 technology.Groups included a control group(wildtype),CyPA knockout group,traditional Chinese medicine(TCM)extract with Fuzhengzengxiao decoction group,and TCM extract with Fuzhengzengxiao decoction t CyPA knockout group.Each group was exposed to radiation at doses of 0,2,4,6,and 8 Gy.After 24 h,MTT assays were used to determine the survival rate of lung cancer cells and calculate radiosensitivity.The qPCR was used to measure mRNA expression of DDIT3,CDKN1A,and CDC25A associated with DNA damage repair.Results:Without irradiation,Fuzhengzengxiao decoction reduced the survival rate of lung adenocarcinoma cells(P<.0001).After irradiation,TCM extract with Fuzhengzengxiao decoction,CyPA knockout,and TCM extract with Fuzhengzengxiao decoction t CyPA knockout groups had reduced survival rates(P<.0001)and radiosensitivity was increased significantly.Expression of DDIT3,CDKN1A,and CDC25A was upregulated after knockout of CyPA(P<.0001).Expression of DDIT3 and CDC25A was increased after irradiation in wildtype cells treated with TCM extract with Fuzhengzengxiao decoction(DDIT3,P<.0001;CDC25A,P紏.0059).The TCM extract with Fuzhengzengxiao decoction t CyPA knockout group also had increased expression of DDIT3 and CDC25A after irradiation(P<.0001).Conclusion:Fuzhengzengxiao decoction significantly decreases the survival rate of lung cancer cells,and its mechanism may be related to radiosensitization by decreasing expression of CyPA and inducing G1/S cell cycle arrest. | Jinkun Ma Lin Xu Jinchang Huang Qiaoli Zhang Yuqin Qiu Xuewei Qi Lina Wang Yuchan Cao Zeyu Liu Shiyun Liu | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 1 |
| 20 | Effects of annealing treatment and pH on preparation of cit- rate stabilized PtRu/C catalyst显示文摘 | Xu Yuqin Xie Xiaofeng Guo Jianwei | 2006 | Journal of Pow- er Sources2006，162，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员