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| 1 | 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana显示文摘5-Methylcytosine (m 5 C ) 是描绘得好的 DNA 修正,并且主要也在初核质和优核质在丰富的非编码的 RNA 被报导。然而,分发和 m 5 在植物 mRNAs 的 C 仍然保持大部分未知。这里,我们报导 RNA m 5 在由使用 m 5 C RNA immunoprecipitation 由一条深定序的途径列在后面(m 5 C-RIP-seq ) 。LC-MS/MS 和点污点分析揭示 m 5 在各种各样的纸巾并且在不同发展阶段的 C mRNA 修正。m 5 C-RIP-seq 分析识别了 6045 m 5 在 4465 的 C 山峰在年轻幼苗表示了基因。我们发现那 m 5 C 在编码序列,二座山峰在开始 codons 以后并且在站 codons 前立即定位了被充实,并且随低翻译与 mRNAs 被联系活动。我们进一步表明了那 RNA (cytosine-5 )-methyltransferase,tRNA 特定的 methyltransferase 4B (TRM4B ) ,展览 m 5 C RNA methyltransferase 活动。在 TRM4B 的变化在根开发和减少的 m 5 C 山峰。TRM4B 影响涉及根开发的基因的抄本层次,它断然与他们的 mRNA 稳定性和 m 5 C 层次。我们的结果建议那 m 5 在 mRNA 的 C 是新 epitranscriptome 标记 inArabidopsis,并且这修正的那条规定是基因的不可分的部分规章的网络位于 \O 下面植物开发。 | Xuean Cui Zhe Liang Lisha Shen Qian Zhang Shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu | 2017 | Molecular Plant2017，10，11： | 19 |
| 2 | An H3K27me3 demethylase-HSFA2 regulatory loop orchestrates transgenerational thermomemory in Arabidopsis显示文摘Global warming has profound effects on plant growth and fitness.Plants have evolved sophisticated epigenetic machinery to respond quickly to heat,and exhibit transgenerational memory of the heat-induced release of post-transtriptional gene silencing(PTGS).However,how thermomemory is transmitted to progeny and the physiological relevance are elusive.Here we show that heat-induced HEAT SHOCK TRANSCRIPTION FACTOR A2(HSFA2)directly activates the H3K27me3 demethylase RELATIVE OF EARLY FLOWERING 6(REF6),which in turn derepresses HSFA2.REF6 and HSFA2 establish a heritable feedback loop,and activate an E3 ubiquitin ligase,SUPPRESSOR OF GENE SILENCING 3(SGS3)-INTERACTING PROTEIN 1(SGIP1).SGIP1-mediated SGS3 degradation leads to inhibited biosynthesis of trans-acting siRNA(tasiRNA).The REF&HSFA2 loop and reduced tasiRNA converge to release HEAT-INDUCED TAS1 TARGET 5(HTT5),which drives early flowering but attenuates immunity.Thus,heat induces transmitted phe no types via a coordi nated epigenetic network involvi ng histone demethylases,tran scriptio n factors,and tasiRNAs,ensuring reproductive success and transgenerational stress adaptation. | Junzhong Liu Lili Feng Xueting Gu Xian Deng Qi Qiu Qun Li Yingying Zhang Muyang Wang Yiwen Deng Ertao Wang Yuke He Isabel Baurle Jianming Li Xiaofeng Cao Zuhua He | 2019 | Cell Research2019，29，5： | 10 |
| 3 | WSe2 2D p-type semiconductor-based electronic devices for information technology:Design,preparation,and applications显示文摘The pioneering exfoliation of monolayer tungsten diselenide has greatly inspired researchers toward semiconducting applications.WSe2 belongs to a family of transition-metal dichalcogenides.Similar to graphene,WSe2 and analogous dichalcogenides have layered structures with weak van der Waals interactions between two adjacent layers.First,the readers are presented with the fundamentals of WSe2,such as types,morphologies,and properties.Here,we report the characterization principles and practices such as microscopy,spectroscopy,and diffraction.Second,the methods for obtaining high-quality WSe2,such as exfoliation,hydrothermal and chemical vapor deposition,are briefly listed.With advantages of light weight,flexibility,and high quantum efficiency,2D materials may have a niche in optoelectronics as building blocks in p-n junctions.Therefore,we introduce a state-of-the-art demonstration of heterostructure devices employing the p-type WSe2 semiconductor.The device architectures include field-effect transistors,photodetectors,gas sensors,and photovoltaic solar cells.Due to its unique electronic,optical,and energy band properties,WSe2 has been increasingly investigated due to the conductivity of the p-type charge carrier upon palladium contact.Eventually,the dynamic research on WSe2 and van der Waals heterostructures is summarized to arouse the passion of the 2D research community. | Qilin Cheng Jinbo Pang Dehui Sun Jingang Wang Shu Zhang Fan Liu Yuke Chen Ruiqi Yang Na Liang Xiheng Lu Yanchen Ji Jian Wang Congcong Zhang Yuanhua Sang Hong Liu Weijia Zhou | 2020 | InfoMat2020，2，4： | 5 |
| 4 | Effects of Neo-Tethyan evolution on the petroleum system of Persian Gulf Superbasin显示文摘Considering the Neo-Tethyan tectonic process and the resulting environmental changes,a geodynamic model of“one-way train loading”is proposed to analyze the formation and evolution mechanism of the Persian Gulf Superbasin with the most abundant hydrocarbons in the world.The Persian Gulf Superbasin has long been in a passive continental margin setting since the Late Paleozoic in the process of unidirectional subduction,forming a superior regional space of hydrocarbon accu-mulation.During the Jurassic-Cretaceous,the Persian Gulf Superbasin drifted slowly at low latitudes,and developed multiple superimposed source-reservoir-caprock assemblages as a combined result of several global geological events such as the Hadley Cell,the Equatorial Upwelling Current,and the Jurassic True Polar Wander.The collision during the evolution of the foreland basin since the Cenozoic led to weak destruction,which was conducive to the preservation of oil and gas.Accordingly,it is be-lieved that the slow drifting and long retention in favorable climate zone of the continent are the critical factors for hydrocarbon enrichment.Moreover,the prospects of hydrocarbon potential in other continents in the Neo-Tethyan were proposed. | ZHU Rixiang ZHANG Shuichang WAN Bo ZHANG Wang LI Yong WANG Huajian LUO Beiwei LIU Yuke HE Zhiliang JIN Zhijun | 2023 | Petroleum Exploration and Development2023，50，1： | 4 |
| 5 | miR-4458 inhibits the epithelial-mesenchymal transition of hepatocellular carcinoma cells by suppressing the TGF-β signaling pathway via targeting TGFBR1显示文摘Hepatocellular carcinoma(HCC)is one of the most lethal cancers in the world.MicroRNAs play a pivotal role in the progression of various cancers.To date,very lttle attention has beerl paid to miR-4458.Therefore,the aim of our study was to explore the function and underlying molecular mech-anism of miR-4458 in HCC.We found that the expression of miR-4458 was reduced in HCC tissues and cell lines.Forced overexpression of miR-4458 inhibited the migration,invasion,and epithelial-mesenchymal transition(EMT)of HCC cells,while downregulation of miR-4458 promoted the aggressive phenotype.Furthermore,transforming growth factor beta receptor 1(TGFBR1),the modulator of the TGF-βsignaling pathway,was verified to be a novel target gene of miR-4458 by dual-luciferase reporter gene assay.Upregulated miR-4458 dramatically abolished TGFBR1 and p-Smad2/3 expression,thus blocking the TGF-βsig naling pathway.Moreover,restoration of TGFBR1 partially rescued the miR-4458-mediated suppressive effect on the migration,invasion,and EMT and reactivated the TGFβsignaling pathway in HCC cells.In summary,our findings first demonstrated a mechanism of miR 4458 in HCC cell migration,invasion,and EMT by regulating the TGFβsignaling pathway via directly targeting TGFBR1. | Yuke Zhang Kun Shi Hang Liu Wei Chen Yunhai Luo Xufu Wei Zhongjun Wu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 4 |
| 6 | METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most prevalent cancers in the world,with a high likelihood of metastasis and a dismal prognosis.The reprogramming of glucosemetabolism is critical in the development ofHCC.TheWarburg effect has recently been confirmed to occur in a variety of cancers,including HCC.However,little is known about the molecular biological mechanisms underlying the Warburg effect in HCC cells.In this study,we sought to better understand how methyltransferase 5,N6-adenosine(METTL5)controls the development of HCC and theWarburg effect.Methods:In the current study,quantitative real-time polymerase chain reaction and Western blotting were used to detect the expression of METTL5 in HCC tissues and cell lines.Several different cell models and animal models were established to determine the role of METTL5 in glucose metabolism reprogramming and the underlying molecularmechanism of HCC.Glutathione-S-transferase pulldown,coimmunoprecipitation,RNA sequencing,non-targeted metabolomics,polysome profiling,and luciferase reporter assays were performed to investigate the molecular mechanisms of METTL5 in HCC cells.Results:We discovered that METTL5 drove glucose metabolic reprogramming to promote the proliferation and metastasis of HCC.Mechanistically,upregulation of METTL5 promoted c-Myc stability and thus activated its downstream glycolytic genes lactate dehydrogenase A(LDHA),enolase 1(ENO1),triosephosphate isomerase 1(TPI1),solute carrier family 2 member 1(SLC2A1),and pyruvate kinase M2(PKM2).The c-Box and ubiquitin binding domain(UBA)regions of ubiquitin specific peptidase 5(USP5)binded to c-Myc protein and inhibited K48-linked polyubiquitination of c-Myc.Further study revealed that METTL5 controled the USP5 translation process,which in turn regulated the ubiquitination of c-Myc.Furthermore,we identified cAMP responsive element binding protein 1(CREB1)/P300 as a critical transcriptional regulator ofMETTL5 that promoted the transcription of METTL5 in HCC.In patient-derived tumor xenograft(PDX)models,adenovirus-mediated knockout of METTL5 had a good antitumor effect and prolonged the survival of PDX-bearing mice.Conclusions:These findings point to a novel mechanism by which CREB1/P300-METTL5-USP5-c-Myc controls abnormal glucose metabolism and promotes tumor growth,suggesting that METTL5 is a potential therapeutic target and prognostic biomarker for HCC. | Peng Xia Hao Zhang Haofeng Lu Kequan Xu Xiang Jiang Yuke Jiang Xiangdong Gongye Zhang Chen Jie Liu Xi Chen Weijie Ma Zhonglin Zhang Yufeng Yuan | 2023 | Cancer Communications2023，43，3： | 3 |
| 7 | Self-Sensing Test Method for the Temperature of Piezoelectric Stacks显示文摘A self-sensing test method for the temperature of piezoelectric stack,based on the high correlation between the static capacitance and the stack temperature,is proposed in order to construct a self-sufficient methodology of temperature measurement. Firstly,a theoretical model of static capacitance of the piezoelectric stack under preload was set up,and the influence of preload on the static capacitance was analyzed. Secondly,the correctness of the model was verified by static capacitance test experiments under various preloading conditions. Finally, the temperature measurement experiments at low-temperature stage for two kinds of piezoelectric stacks,namely the lowtemperature-resistant piezoelectric stack and conventional piezoelectric stack, were conducted under various preloading conditions using a polynomial fitting method. The results,which validate the accuracy of the test method,show that the maximum temperature deviations of the two kinds of piezoelectric stack are 3.9 ℃ and 2.8 ℃,respectively,when the preload force is close to the specified value. The test method uses the piezoelectric stack itself as a temperature sensor,which does not require additional equipment for temperature sensing,so that the space and equipment cost could be economized. And the test for static capacitance is concise and convenient,which indicates that in the cooling process,a concise and efficient test of the temperature of the piezoelectric stack could be realized so as to grasp the current temperature change in time. | SHEN Xing LI Yang LIANG Lei ZHANG Lei DAI Yuke CHEN Yuchen YANG Jinchuan | 2019 | Transactions of Nanjing University of Aeronautics and Astronautics2019，36，1： | 2 |
| 8 | Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis显示文摘RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers.However,efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers remains challenging.Recently,more and more attention has been paid to applying nucleic acid nanoassemblies in diagnosing and treating.Due to the flexibility and deformability of nucleic acids,the nanoassemblies could be fabricated with different shapes and structures.With hybridization,nucleic acid nanoassemblies,including DNA and RNA nanostructures,can be applied to enhance RNA therapeutics and diagnosis.This review briefly introduces the construction and properties of different nucleic acid nanoassemblies and their applications for RNA therapy and diagnosis and makes further prospects for their development. | Mengnan Zhao Rujing Wang Kunmeng Yang Yuhong Jiang Yachen Peng Yuke Li Zhen Zhang Jianxun Ding Sanjun Shi | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 2 |
| 9 | Fine-scale activity patterns of large-and medium-sized mammals in a deciduous broadleaf forest in the Qinling Mountains,China显示文摘The composition of animal species and interactions among them are widely known to shape ecological communities and fine-scale(e.g.,<1 km)monitoring of animal communities is essential for understanding the relationships among animals and plants.Although the co-existence of large-and medium-sized species has been studied across different scales,research on fine-scale interactions of herbivores in deciduous broadleaf forests is limited.Camera trapping of large-and medium-sized mammals was carried out over a 1 year period within a 25 ha deciduous broadleaf forest dynamics plot in the Qinling Mountains,China.Fourteen species of large-and medium-sized mammals,including six carnivores,six ungulates,one primate and one rodent species were found.Kernel density estimations were used to analyse the diel or 24 h activity patterns of all species with more than 40 independent detections and general linear models were developed to explore the spatial relationships among the species.The combination of overlapping diel activity patterns and spatial associations showed obvious niche separation among six species:giant panda(Ailuropoda melanoleuca David),takin(Budorcas taxicolor Hodgson),Reeves’s muntjac(Muntiacus reevesi Ogilby),tufted deer(Elaphodus cephalophus Milne-Edwards),Chinese serow(Capricornis milneedwardsii David)and wild boar(Sus scrofa Linnaeus).Long-term fine-scale monitoring is useful for providing information about the co-existence of species and their interactions.The results demonstrate the importance for fine-scale monitoring of animals and plants for improving understanding of species interactions and community dynamics. | Yuke Zhang Xiangbo He Xuehua Liu Melissa Songer Haishan Dang Quanfa Zhang | 2021 | Journal of Forestry Research2021，32，6： | 1 |
| 10 | Distinct mechanism of receptor and nonreceptor tyrosine kinase activation by reactive oxygen species in vascular smooth muscle cells: role of metalloprotease and protein kinase Cδ 显示文摘 | Laura J Bloem Lan Yu | 2003 | Mol Cell Biol2003，23，1： | 1 |
| 11 | A new method of activated carbon loading MnO2 to formaldehyde degradation显示文摘 | Yang Yuke Zhang Hua Jin Shaogang | | 0，，： | 1 |
| 12 | PRMT6 physically associates with nuclear factor Y to regulate photoperiodic flowering in Arabidopsis显示文摘The timing of floral transition is critical for reproductive success in flowering plants.In long-day(LD)plant Arabidopsis,the floral regulator gene FLOWERING LOCUS T(FT)is a major component of the mobile florigen.FT expression is rhythmically activated by CONSTANS(CO),and specifically accumu-lated at dusk of LDs.However;the underlying mechanism of adequate regulation of FT transcription in response to day-length cues to warrant flowering time still remains to be investigated.Here,we identify a homolog of human protein arginine methyltransferases 6(HsPRMT6)in Arabidopsis,and confirm AtPRMT6 physically interacts with three positive regulators of flowering Nuclear Factors YC3(NF-YC3),NF-YC9,and NF-YB3.Further investigations find that AtPRMT6 and its encoding protein accumulate at dusk of LDs.PRMT6-mediated H3 R2me2a modification enhances the promotion of NF-YCs on FT transcription in response to inductive LD signals.Moreover,AtPRMT6 and its homologues proteins AtPRMT4a and AtPRMT4b coordinately inhibit the expression of FLOWERING LOCUS C,a suppressor of FT.Taken together,our study reveals the role of arginine methylation in photoperiodic pathway and how the PRMT6-mediating H3R2me2a system interacts with NF-CO module to dynamically control FT expression and facilitate flowering time. | Pingxian Zhang Xiulan Li Yifan Wang Weijun Guo Sadaruddin Chachar Adeel Riaz Yuke Geng Xiaofeng Gu Liwen Yang | 2021 | aBIOTECH2021，2，4： | 1 |
| 13 | Behavior and functional modeling methods of doubly salient electromagnetic generators for aircraft electrical power system applications显示文摘The Doubly Salient Electromagnetic Generator(DSEG) is a promising candidate in aircraft generator application due to the simplicity, robustness and reliability. However, the field windings and the armature windings are strongly coupled, which makes the inductance characteristics non-linear and too complex to model. The complex model with low precision also leads to difficulties in modeling and analysis of the entire aircraft Electrical Power System(EPS). A behavior level modeling method based on modified inductance Support Vector Machine(SVM) is proposed. The Finite Element Analysis(FEA) inductance data are modified based on the experiment results to improve the precision. A functional level modeling method based on input–output characteristics SVM is also proposed. The two modeling methods are applied to a 9 kW DSEG prototype. The steady state and transient process precision of the proposed methods are proved by comparing with the experiment results. Meanwhile, the modeling time consumption, the application time consumption and the calculation resource demand are compared. The DSEG behavior and functional modeling methods provide precious results with high efficiency, which accelerates theoretical analysis and expands the application foreground of the DSEG in the aircraft EPS. | Yanwu XU Zhuoran ZHANG Li YU Yuke SHI | 2019 | Chinese Journal of Aeronautics2019，32，2： | 1 |
| 14 | Water-dominated vegetation activity across biomes in mid-latitudinal eastern China显示文摘 | Wu Xiuchen Liu Hongyan Ren Ji He Siyuan Zhang Yuke | 2009 | Geophysical Research Letters2009，，4： | 1 |
| 15 | Gulong shale oil enrichment mechanism and orderly distribution of conventional–unconventional oils in the Cretaceous Qingshankou Formation,Songliao Basin,NE China显示文摘Through the study of organic matter enrichment,hydrocarbon generation and accumulation process of black shale of the Cretaceous Qingshankou Formation in the Songliao Basin,the enrichment mechanism of Gulong shale oil and the distribution of conventional–unconventional oil are revealed.The Songliao Basin is a huge interior lake basin formed in the Early Cretaceous under the control of the subduction and retreat of the western Pacific plate and the massive horizontal displacement of the Tanlu Fault Zone in Northeast China.During the deposition of the Qingshankou Formation,strong terrestrial hydrological cycle led to the lake level rise of the ancient Songliao Basin and the input of a large amount of nutrients,resulting in planktonic bacteria and algae flourish.Intermittent seawater intrusion events promoted the formation of salinization stratification and anoxic environment in the lake,which were beneficial to the enrichment of organic matters.Biomarkers analysis confirms that the biogenic organic matter of planktonic bacteria and algae modified by microorganisms plays an important role in the formation of high-quality source rocks with high oil generation capability.There are four favorable conditions for the enrichment of light shale oil in the Qingshankou Formation of the Gulong Sag,Songliao Basin:the moderate organic matter abundance and high oil potential provide sufficient material basis for oil enrichment;high degree of thermal evolution makes shale oil have high GOR and good mobility;low hydrocarbon expulsion efficiency leads to a high content of retained hydrocarbons in the source rock;and the confinement effect of intra-layer cement in the high maturity stage induces the efficient accumulation of light shale oil.The restoration of hydrocarbon accumulation process suggests that liquid hydrocarbons generated in the early(low–medium maturity)stage of the Qingshankou Formation source rocks accumulated in placanticline and slope after long-distance secondary migration,forming high-quality conventional and tight oil reservoirs.Light oil generated in the late(medium–high maturity)stage accumulated in situ,forming about 15 billion tons of Gulong shale oil resources,which finally enabled the orderly distribution of conventional–unconventional oils that are contiguous horizontally and superposed vertically within the basin,showing a complete pattern of“whole petroleum system”with conventional oil,tight oil and shale oil in sequence. | ZHANG Shuichang ZHANG Bin WANG Xiaomei FENG Zihui HE Kun WANG Huajian FU Xiuli LIU Yuke YANG Chunlong | 2023 | Petroleum Exploration and Development2023，50，5： | 0 |
| 16 | Palladium-Catalyzed One-Step Synthesis of Stereodefined Difunctionalized Glycals显示文摘Efficiently modifying glycals by directly introducing functional groups into their double bonds is a longstanding challenge.Here,the strategy of introducing two different functional groups into 1-iodoglycals to obtain modified glycals in one step through palladium catalysis was reported for the first time,and the modified glycals contained stereodefined tetrasubstituted olefins.Using this method,various difunctionalized glycals that were difficult to form by other routes were synthesized in moderate to good yields.Control experiments and density functional theory calculations show that the palladium catalyst played dual roles in this transformation,namely,inducing nucleophilic substitution and catalyzing Suzuki coupling.The reaction intermediate was isolated and confirmed by X-ray crystallographic analysis.Furthermore,the gram-scale synthesis and facile deprotection of the target compound enhances the practicality of this strategy. | Xiao-Ping Gong Yuke Li Hong-Chao Liu Zhe Zhang Zhi-Jie Niu Ya-Nan Ding Yang An Xi Chen Yan-Chong Huang Rui-Qiang Jiao Xue-Yuan Liu Yong-Min Liang | 2023 | CCS Chemistry2023，5，3： | 0 |
| 17 | TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity显示文摘Drought is a major environmental stress limiting global wheat(Triticum aestivum)production.Exploring drought tolerance genes is important for improving drought adaptation in this crop.Here,we cloned and characterized TaTIP41,a novel drought tolerance gene in wheat.TaTIP41 is a putative conserved component of target of rapamycin(TOR)signaling,and the Ta TIP41 homoeologs were expressed in response to drought stress and abscisic acid(ABA).The overexpression of Ta TIP41 enhanced drought tolerance and the ABA response,including ABA-induced stomatal closure,while its downregulation using RNA interference(RNAi)had the opposite effect.Furthermore,Ta TIP41 physically interacted with TaTAP46,another conserved component of TOR signaling.Like TaTIP41,TaTAP46 positively regulated drought tolerance.Furthermore,TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase(PP2A)catalytic subunits,such as TaPP2A-2,and inhibited their enzymatic activities.Silencing TaPP2A-2 improved drought tolerance in wheat.Together,our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat,and their potential application in improving wheat environmental adaptability. | Jianhui Ma Yuke Geng Hong Liu Mengqi Zhang Shujuan Liu Chenyang Hao Jian Hou Youfu Zhang Daijing Zhang Weijun Zhang Xueyong Zhang Tian Li | 2023 | Journal of Integrative Plant Biology2023，65，9： | 0 |
| 18 | A discussion of irrational stockpiling behaviour during crisis显示文摘One of the ubiquitous human behaviours observed in natural disasters and humanitarian crisis is irrational stock-piling(also known as hoarding or panic buying).Limited,distorted and exaggerated information during crisis disturbs people’s judgement and results in aberrant actions which can be explained with economics and psychol-ogy theories.The objective of this paper is to examine the perplexing stockpiling phenomena during disasters like COVID-19 pandemic and discuss its immediate and long-term impact on economy,society and local communities. | Yiqun Chen Abbas Rajabifard Soheil Sabri Katie Elisabeth Potts Farhad Laylavi Yuke Xie Yibo Zhang | 2020 | Journal of Safety Science and Resilience2020，1，1： | 0 |
| 19 | SpectraTr:A novel deep learning model for qualitative analysis of drug spectroscopy based on transformer structure显示文摘The drug supervision methods based on near-infrared spectroscopy analysis are heavily dependent on the chemometrics model which characterizes the relationship between spectral data and drug categories.The preliminary application of convolution neural network in spectral analysis demonstrates excellent end-to-end prediction ability,but it is sensitive to the hyper-parameters of the network.The transformer is a deep-learning model based on self-attention mechanism that compares convolutional neural networks(CNNs)in predictive performance and has an easy-todesign model structure.Hence,a novel calibration model named SpectraTr,based on the transformer structure,is proposed and used for the qualitative analysis of drug spectrum.The experimental results of seven classes of drug and 18 classes of drug show that the proposed SpectraTr model can automatically extract features from a huge number of spectra,is not dependent on pre-processing algorithms,and is insensitive to model hyperparameters.When the ratio of the training set to test set is 8:2,the prediction accuracy of the SpectraTr model reaches 100%and 99.52%,respectively,which outperforms PLS DA,SVM,SAE,and CNN.The model is also tested on a public drug data set,and achieved classification accuracy of 96.97%without preprocessing algorithm,which is 34.85%,28.28%,5.05%,and 2.73%higher than PLS DA,SVM,SAE,and CNN,respectively.The research shows that the SpectraTr model performs exceptionally well in spectral analysis and is expected to be a novel deep calibration model after Autoencoder networks(AEs)and CNN. | Pengyou Fu Yue Wen Yuke Zhang Lingqiao Li Yanchun Feng Lihui Yin Huihua Yang | 2022 | Journal of Innovative Optical Health Sciences2022，15，3： | 0 |
| 20 | Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity显示文摘Neural progenitor cells(NPC)are those that are the source of neural cells for cell transplantation and gene therapy.The shortage in quantity and the limited life spans of primary cultured cells limit its widespread use in basic research.Immortalized NPC,which also possess the capacity of self-renewal and can proliferate infinitely,can produce a large number of NPCs with stable phenotype and genotype.Here we report that an immortalized neural progenitor cell strain,which we named as INPC,was suc-cessfully established by gene-transfer of simian virus 40 large T antigen gene mediated by liposomes.The INPC retained the biological characteristics of its original cells and provided a safe and reliable cell platform for the treat-ment of central nervous system diseases and transgenic cell transplantation.INPC could express low levels of MHC antigens which was down-regulated after differentiation.This indicates that INPC possesses poor immunogenicity.The immortalized cells may show good long-term survival and do not elicit an acute immunological response follow-ing transplantation. | Feng GAO Yuke TIAN Hui YANG Ke AN Ying XU Xuebi TIAN Chuanhan ZHANG | 2008 | Frontiers of Medicine2008，2，1： | 0 |
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