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| 1 | Metabolic analyses reveal different mechanisms of leaf color change in two purple-leaf tea plant (Camellia sinensis L.) cultivars显示文摘Purple-leaf tea plants,as anthocyanin-rich cultivars,are valuable materials for manufacturing teas with unique colors or flavors.In this study,a new purple-leaf cultivar“Zixin”(“ZX”)was examined,and its biochemical variation and mechanism of leaf color change were elucidated.The metabolomes of leaves of“ZX”at completely purple,intermediately purple,and completely green stages were analyzed using ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF-MS).Metabolites in the flavonoid biosynthetic pathway remained at high levels in purple leaves,whereas intermediates of porphyrin and chlorophyll metabolism and carotenoid biosynthesis exhibited high levels in green leaves.In addition,fatty acid metabolism was more active in purple leaves,and steroids maintained higher levels in green leaves.Saponin,alcohol,organic acid,and terpenoid-related metabolites also changed significantly during the leaf color change process.Furthermore,the substance changes between“ZX”and“Zijuan”(a thoroughly studied purple-leaf cultivar)were also compared.The leaf color change in“Zijuan”was mainly caused by a decrease in flavonoids/anthocyanins.However,a decrease in flavonoids/anthocyanins,an enhancement of porphyrin,chlorophyll metabolism,carotenoid biosynthesis,and steroids,and a decrease in fatty acids synergistically caused the leaf color change in“ZX”.These findings will facilitate comprehensive research on the regulatory mechanisms of leaf color change in purple-leaf tea cultivars. | Jiazhi Shen Zhongwei Zou Xuzhou Zhang Lin Zhou Yuhua Wang Wanping Fang Xujun Zhu | 2018 | Horticulture Research2018，5，1： | 22 |
| 2 | Exogenous abscisic acid significantly affects proteome in teaplant (Camellia sinensis) exposed to drought stress显示文摘Tea[Camellia sinensis(L.)O.Kuntze]is an important economic crop,and drought is the most important abiotic stress affecting yield and quality.Abscisic acid(ABA)is an important phytohormone responsible for activating drought resistance.Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes,along with crop management practices that can mitigate drought stress.The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.Leaf protein patterns of tea plants under simulated drought stress[(polyethylene glycol(PEG)-treated]and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis(2-DE),followed by matrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF)mass spectrometry(MS).Among the 72 protein spots identified by MALDI-TOF MS,16 proteins were downregulated and two were upregulated by exogenous ABA.The upregulated proteins have roles in glycolysis and photosystem II stabilization.Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism,control of reactive oxygen species(ROS),defense,signaling or nucleic acid metabolism.The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress.The results support the importance of the role that ABA plays in the tea plant during drought stress,by improving protein transport,carbon metabolism and expression of resistance proteins. | Lin Zhou Hui Xu Sue Mischke Lyndel W Meinhardt Dapeng Zhang Xujun Zhu Xinghui Li Wanping Fang | 2014 | Horticulture Research2014，1，1： | 12 |
| 3 | Synthesis of barium strontium titanate nanorods in reverse microemulsion 显示文摘 | Wanping Chen Qian Zhu | 2007 | Materials Letters2007，61，： | 1 |
| 4 | A propagation model of computer virus with nonlinear vaccination probability显示文摘 | Chenquan Gan Xiaofan Yang Wanping Liu Qingyi Zhu | 2013 | Communications in Nonlinear Science and Numerical Simulation2013，，： | 1 |
| 5 | Synthesis of barium strontium titanate nanorods in reverse microemulsion 显示文摘 | Wanping Chen Qi' an Zhu | 2007 | Materials Letters2007，61，16： | 1 |
| 6 | Synthesis of barium strontium titanatc nanorods in reverse microemulsion显示文摘 | CHEN Wanping ZHU Qi'an | 2007 | Matcrials Letters2007，61，16： | 1 |
| 7 | Recognition for avian influenza virus proteins based on support vector machine and linear discriminant analysis显示文摘Total 200 properties related to structural characteristics were employed to represent structures of 400 HA coded proteins of influenza virus as training samples. Some recognition models for HA proteins of avian influenza virus (AIV) were developed using support vector machine (SVM) and linear discriminant analysis (LDA). The results obtained from LDA are as follows: the identification accuracy (Ria) for training samples is 99.8% and Ria by leave one out cross validation is 99.5%. Both Ria of 99.8% for training samples and Ria of 99.3% by leave one out cross validation are obtained using SVM model, respectively. External 200 HA proteins of influenza virus were used to validate the external predictive power of the resulting model. The external Ria for them is 95.5% by LDA and 96.5% by SVM, respectively, which shows that HA proteins of AIVs are preferably recognized by SVM and LDA, and the performances by SVM are superior to those by LDA. | LIANG GuiZhao CHEN ZeCong YANG ShanBin MEI Hu ZHOU Yuan YANG Li ZHOU Peng YANG ShengXi SHU Mao LIAO ChunYang WU ShiRong LI GenRong HE Liu GAO JianKun Gan MengYu LI DeJing CHEN GuoPing WANG GuiXue LONG Sha JING JuHua ZHENG XiaoLin ZENG Hui ZHANG QiaoXia ZHANG MengJun YANG Qi TIAN FeiFei TONG JianBo WANG JiaoNa LIU YongHong LI Bo QIU LiangJia CAI ShaoXi ZHAO Na YANG Yan SU XiaLi SONG Jian CHEN MeiXia ZHANG XueJiao SUN JiaYing LI JingWei CHEN GuoHua CHEN Gang DENG Jie PENG ChuanYou ZHU WanPing XU LuoNan WU YuQuan LIAO LiMin LI Zhi LI Jun LU DaJun SU QinLiang HUANG ZhengHu ZHOU Ping LI ZhiLiang | 2008 | Science China Chemistry2008，51，2： | 0 |
| 8 | Preparation and Characterization of Nanostructured Lipid Carrier Loaded with UVA/UVB Filters显示文摘A new photoprotective system based on encapsulating UVA (butyl methoxydibenzoylmethane, BMBM) and UVB (octyl methoxycinnamate, OMC) filters into nanostructured lipid carriers (NLC) has been prepared to develop cosmetic formulations with effective UV protection. BMBM/OMC-loaded NLC was prepared by ultrasonication-homogenisation, and analysed by particle size, zeta potential (ZP), encapsulation efficiency (EE), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). Moreover, the UV protection property and photostability were investigated and compared with BMBM/OMC-conventional emulsions. The particle size and ZP of BMBM/OMC-loaded NLC were 310.24 nm and -33.6 mV, EE of BMBM and OMC were 85.46% and 99.32%. SEM, FTIR and DSC analysis confirmed BMBM and OMC entrapped in the lipid matrix core and the structure was stable during storage. Compared with conventional emulsion, BMBM/OMC-loaded NLC displayed perfect photo protection property in whole UV range. The photostability studies showed that the NLC can improve the photostability of sunscreens. | Zhang Qianjie Zhao Xiaowei Song Lili Ou Wenhua Zhang WanPing Zhu Haiyang | 2018 | China Detergent & Cosmetics2018，3，4： | 0 |
| 9 | Progress and perspective on intercropping patterns in tea plantations显示文摘Intercropping,as one of the complex ecological cultivations,is an important tea plantation pattern.Compared to sole-cropping tea plantations,intercropping can improve the above-and below-ground environment,which is beneficial to tea plant growth,the formation of high tea quality and the increase of tea yield.In this review,we summarized the impacts of intercropping on the tea plantation environment(microclimate,biomass,soil nutrients,microorganisms and heavy metals),tea plants growth and tea yield.We then analyzed how intercropping affects the growth and metabolism of tea plants based on the impact of intercropping on the environment.As a result,the achievements and progress of tea plantation intercropping are summarized,the remaining problems of the current research on intercropping tea plantations are highlighted,and provide new insights for the advanced research of intercropping in tea plantations. | Xiaogang Lei Ting Wang Bin Yang Yu Duan Lin Zhou Zhongwei Zou Yuanchun Ma Xujun Zhu Wanping Fang | 2022 | Beverage Plant Research2022，2，1： | 0 |
| 10 | Targeted metabolomics reveals dynamic changes during the manufacturing process of Yuhua tea,a stir-fried green tea显示文摘The chemical composition of Yuhua tea is in a state of constant change during tea processing.However,the dynamic changes in this complex metabolic process remain unclear.Therefore,we detected the changes of compounds in the five stages of processing of Yuhua tea,and carried out metabolomic analysis based on UPLC–MS.In total,898 metabolites were detected in the five different stages.In the spreading and fixation processes,the differences in metabolites were the most significant,with 127 and 150 metabolites,respectively,undergoing significant changes.During the spreading period,the levels of amino acids and their derivatives,as well as flavonoids,increased sharply,and most continued to increase or stabilized after spreading.In addition,only 26 and 95 metabolites changed significantly during the rolling and drying processes,respectively.Orthogonal projections to latent structures-discriminant analysis showed that the metabolomics of tea changed significantly during the manufacturing process,especially amino acids and derivatives,flavonoids and lipids.This study provides a comprehensive overview of the metabolic changes during the processing of stir-fried green tea,which has potential significance for quality control and flavor improvement of tea. | Kexin Zhang Taiyu Ren Jieren Liao Shan Wang Zhongwei Zou Yuanchun Ma Fang Li Wanping Fang Xujun Zhu | 2021 | Beverage Plant Research2021，1，1： | 0 |
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