|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | PTEN encoding product:a marker for tumorigenesis and progression of gastric carcinoma显示文摘AIM: To detect the expression of PTEN encoding productin normal mucosa, intestinal metaplasia (IM), dysplasia andcarcinoma of the stomach, and to investigate its clinicalimplication in tumorigenesis and progression of gastriccarcinoma.METHODS: Formalin-fixed paraffin embedded specimens from184 cases of gastric carcinoma, their adjacent normal mucosa,IM and dysplasia were evaluated for PTEN protein expressionby SABC immunohistochemistry. PTEN expression wascompared with tumor stage, lymph node metastasis, Lauren'sand WHO's histological classification of gastric carcinoma.Expression of VEGF was also detected in 60 cases of gastriccarcinoma and its correlation with PTEN was concerned.RESULTS: The positive rates of PTEN protein were 100 %(102/102), 98.5 %(65/66), 66.7 % (4/6) and 47.8 %(88/184)in normal mucosa, IM, dysplasia and carcinoma of the stomach,respectively. The positive rates in dysplasia and carcinomawere lower than in normal mucosa and IM (P<0.01).Advanced gastric cancers expressed less frequent PTEN thanearly gastric cancer (42.9 % v567.6 %, P<0.01). The positiverate of PTEN protein was lower in gastric cancer with thanwithout lymph node metastasis (40.3 % v563.3 %, P<0.01).PTEN was less expressed in diffuse-type than in intestinal-type gastric cancer (41.5 % v557.8 %,P<0.05). Signet ringcell carcinoma showed the expression of PTEN at the lowestlevel (25.0 %, 7/28); less than well and moderatelydifferentiated ones (P<0.01). Expression of PTEN was notcorrelated with expression of VEGF (P>0.05).CONCLUSION: Loss or reduced expression of PTEN proteinoccures commonly in tumorigenesis and progression of gastriccarcinoma. It is suggested that PTEN can be an objective markerfor pathologically biological behaviors of gastric carcinoma. | Lin Yang Li-Ge Kuang Hua-Chuan Zheng Jin-Yi Li Dong-Ying Wu Su-Min Zhang Yan Xin No.4 Lab,Cancer Institute,The First Affiliated Hospital,China Medical University,Shenyang 110001,China Ying Chen Shen Yang Gynecology & Obstetrics Hospital,Shenyang 110014,China | 2003 | World Journal of Gastroenterology2003，9，1： | 127 |
| 2 | Clinical Features of Adult/Adolescent Atopic Dermatitis and Chinese Criteria for Atopic Dermatitis显示文摘 | Ping Liu Yan Zhao Zhang-Lei Mu Qian-Jin Lu Qian-Jin L U Li Zhang Xu Yao Min Zheng Yi-Wen Tang Xin-Xiano Lu Xiu-Juan xia You-Kun Lin Yu-Zhen Li Cai-Xia Tu Zhi-Rong Yao Jin-Hua Xu Wei Li Wei Lai Hui-Min Yang Hong-Fu Xie Xiu-Ping Han Zhi-Qiang Xie Xiang Nong Zai-Pei Guo Dan-Qi Deng Tong-Xin Shi Jian-Zhong Zhang | 2016 | Chinese Medical Journal2016，，7： | 64 |
| 3 | Diagnosis, treatment, and prevention of 2019 novel coronavirus infection in children: experts' consensus statement显示文摘Since the outbreak of 2019 novel coronavirus infection (2019-nCoV) in Wuhan City,China,by January 30,2020,a total of 9692 confirmed cases and 15,238 suspected cases have been reported around 31 provinces or cities in China.Among the confirmed cases,1527 were severe cases,171 had recovered and been discharged at home,and 213 died.And among these cases,a total of 28 children aged from 1 month to 17 years have been reported in China.For standardizing prevention and management of 2019-nCoV infections in children,we called up an experts' committee to formulate this experts' consensus statement.This statement is based on the Novel Coronavirus Infection Pneumonia Diagnosis and Treatment Standards (the fourth edition) (National Health Committee) and other previous diagnosis and treatment strategies for pediatric virus infections.The present consensus statement summarizes current strategies on diagnosis,treatment,and prevention of 2019-nCoV infection in children. | Kunling Shen Yonghong Yang Tianyou Wang Dongchi Zhao Yi Jiang Runming Jin Yuejie Zheng Baoping Xu Zhengde Xie Likai Lin Yunxiao Shang Xiaoxia Lu Sainan Shu Yan Bai Jikui Deng Min Lu Leping Ye Xuefeng Wang Yongyan Wang Liwei Gao | 2020 | World Journal of Pediatrics2020，16，3： | 56 |
| 4 | Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo显示文摘 | Lin Y Luo E Chen X Liu L Qiao J Yan Z Li Z Tang W Zheng X Tian W | 2006 | 中国生物学文摘2006，20，5： | 31 |
| 5 | The General Design and Technology Innovations of CAP1400显示文摘行波堆为一次通过式燃料循环反应堆,其利用堆芯自增殖大大降低了对浓缩和后处理的需求。自增殖将次临界换料燃料转化为新的临界燃料,从而使增殖燃烧波得以扩散。该理念建立在增殖燃烧波和燃料的相对移动的基础上。因此,燃料或增殖燃烧波相对于固定的观察器而言是移动的。行波堆最实用的体现就是能够在将核反应保持在同一位置的同时移动燃料——有时行波堆也被称为'驻波堆'。行波堆能够使用换料铀燃料运行,换料铀燃料包括完全贫化铀、天然铀和低浓缩铀燃料(即235U含量为5.5%或更低的燃料),这些燃料通常在快谱中达不到临界状态。轻水反应堆卸出的乏燃料也可以作为行波堆的换料燃料。上述情况均无需后处理即可实现极高的燃料利用率和燃料废物量的显著降低。当换料燃料为贫化铀时,行波堆的最大优势得以实现,即在启动后,无需浓缩设施,就可维持最先启动的反应堆和一连串后续的反应堆的运行。自2006年起,泰拉能源公司(Terra Power)与50多个机构高度协作,开展了概念设计、工程设计和相关技术开发活动,力争到2026年实现将第一个机组投入使用。本文总结了行波堆技术,包括它的发展计划及其进展,分析了行波堆的社会和经济效益。 | Mingguang Zheng Jinquan Yan Jun Shenttu Lin Tian Xujia Wang Zhongming Qiu | 2016 | Engineering2016，2，1： | 27 |
| 6 | Effects of dietary Clostridium butyricum supplementation on growth performance,intestinal development,and immune response of weaned piglets challenged with lipopolysaccharide显示文摘Background: Weanling pigs, with immature immune system and physiological function, usually experience postweaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, and immunity of weaned pigs challenged with lipopolysaccharide(LPS).Methods: In Experiment(Exp.) 1,144 weaned piglets were weaned at 21 d and randomly assigned to six groups,with six replicates per group and four pigs per replicate, receiving a control diet(CON) or diet supplemented with antibiotics(AB) or C. butyricum(CB)(0.1%, 0.2%, 0.4%, or 0.8%), respectively. All diets in Exp. 1 were a highly digestible basal diet, with 3,000 mg/kg zinc oxide supplied in the first 2 wk only. In Exp. 2, 180 piglets were weaned at 21 d and randomly assigned to five groups, with six replicates per group and six pigs per replicate, receiving CON, AB, or CB(0.2%, 0.4%, or 0.6%) diets. The digestibility of diets was lower than those in Exp. 1, and did not include zinc oxide. At 36 d of Exp. 2, 12 piglets were selected from each of the CON and 0.4% CB groups, six piglets were intraperitoneally injected with LPS(50 μg/kg body weight) and the other six piglets with normal saline;animals were killed at 4 h after injection to collect blood, intestine, and digesta samples for biochemical analysis.Results: In Exp. 1, CB and AB diets had no effect on growth performance of piglets. In Exp. 2, 0.4% CB decreased feed-gain ratio(P < 0.1), diarrhea score(P < 0.05), and increased duodenal, jejunal, and ileal villus height and jejunal villus height/crypt depth(P < 0.05). The 0.4% CB decreased the plasma tumor necrosis factor(TNF) α(P < 0.05) but increased ileal mucosa IL-10 and TLR2 mRNA expression(P < 0.05). Furthermore, 0.4% CB altered the microbial profile, with Bacillus and Ruminococcaceae UGG-003 at genus level and Lactobacillus casei and Parasutterella secunda at species level were higher than CON in colonic content(P < 0.05).Conclusions: Dietary C. butyricum supplementation had positive effects on growth of weaned piglets with less digestible diets. There was a tendency to reduce the feed-gain ratio, which could reduce feed costs in pig production. Moreover, C. butyricum decreased post-weaning diarrhea by improving the intestinal morphology,intestinal microflora profile, and immune function. | Ling Chen Shuang Li Jie Zheng Wentao Li Xuemei Jiang Xilun Zhao Jian Li Lianqiang Che Yan Lin Shengyu Xu Bin Feng Zhengfeng Fang De Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 26 |
| 7 | Regulatory effect of a Chinese herbal medicine formula on nonalcoholic fatty liver disease显示文摘BACKGROUND Non-alcoholic fatty liver disease(NAFLD)has become a major cause of chronic liver disease.The Chinese herbal medicine(CHM)Dachaihu decoction(DCHD)has been proved to treat NAFLD with good efficacy in previous studies.Based on the TCM principle of formula formation,we divided DCHD into soothing liver part,invigorating spleen part,and dredging intestine part.Marshall officially proposed the concept of“intestinal-hepatic axis”,which systematically explains the interactions between the intestine and liver.We hypothesized that the effect of CHM on NAFLD is achieved by regulating the liver and intestine.Thus,we aimed to investigate the possible effect of a CHM formula on NAFLD in a rat model.AIM To investigate the effects of a CHM formula(a decoction of Chinese thorowax root,scutellaria root,and white peony root)on NAFLD and its regulatory effect on the“intestinal-liver”axis.METHODS Sixty rats were randomly divided into control,model,pioglitazone hydrochloride(PH),and CHM(a decoction of Chinese thorowax root,scutellaria root,and white peony root)groups.An NAFLD rat model was established using a high-fat highfructose diet for 16 wk.From the 13th week,rats were administered with PH or a decoction of Chinese thorowax,scutellaria,and white peony root(CHM group)for 4 wk.Rats in the control group and model group were administered with an equal volume of distilled water.At the end of the study,blood was collected via the abdominal aorta.Liver tissues were harvested and any morphological changes were observed by hematoxylin-eosin(HE)staining,Oil red O staining,and Masson staining.In addition,blood lipids,liver function markers,and triglyceride(TG)in liver tissues were analyzed.The levels of transforming growth factor-β1(TGF-β1),tumor necrosis factor-α(TNF-α),Toll-like receptor-4(TLR4),and nuclear factor-kappa B(NF-кB)in liver tissues and secreted immunoglobulin A(sIgA)in intestinal tissues were analyzed by ELISA,and protein and mRNA expression of occludin and zonula occludens-1(ZO-1)in the intestine were measured using Western blot and reverse transcriptionquantitative polymerase chain reaction,respectively.The endotoxin level in plasma was detected by endpoint chromogenic assay.RESULTS Compared to the normal control group,the liver coefficient,serum TG,total cholesterol(TC),low density lipoprotein(LDL),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),blood glucose,plasma endotoxin,and the levels of TG,TNF-α,TGF-β,NF-kB,and TLR4 in liver tissues increased significantly in the model group,while serum high density lipoprotein(HDL),intestinal sIgA,and protein and mRNA expression of occludin and ZO-1 decreased significantly in the model group(P<0.01).PH and CHM attenuated the elevated liver coefficient,serum TG,TC,LDL,AST,and ALT,blood glucose,plasma endotoxin,and the levels of TG,TNF-α,TGF-β,NF-kB,and TLR4 in liver tissues and increased serum HDL levels compared to the model group(P<0.01).Intestinal sIgA and the protein and mRNA expression of intestinal occludin and ZO-1 were significantly increased in the PH group compared to the model and CHM groups(P<0.01).CONCLUSION The decoction of Chinese thorowax root,scutellaria root,and white peony root is beneficial in regulating lipid metabolism and liver function,which indicates that it has a good effect on the liver.To a certain extent,this CHM formula can affect both the liver and intestine,while its effect on the liver is superior to that on the intestine. | Jia-Min Yang Yan Sun Min Wang Xin-Lei Zhang Shu-Jing Zhang Yu-Shan Gao Lin Chen Meng-Yao Wu Lu Zhou Yu-Mei Zhou Yue Wang Feng-Jie Zheng Yu-Hang Li | 2019 | World Journal of Gastroenterology2019，25，34： | 24 |
| 8 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 9 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 10 | Cold adaptation in pigs depends on UCP3 in beige adipocytes显示文摘 | Jun Lin Chunwei Cao Cong Tao Rongcai Ye Meng Dong Qiantao Zheng Chao Wang Xiaoxiao Jiang Guosong Qin Changguo Yan Kui Li John R. Speakman Yanfang Wang Wanzhu Jin Jianguo Zhao | 2017 | Journal of Molecular Cell Biology2017，9，5： | 20 |
| 11 | Multilineage differentiation of adipose-derived stromal cells from GFP transgenic mice显示文摘 | Lin Y Chen X Yan Z Liu L Tang W Zheng X Li Z Qiao J Li S Tian W | 2006 | 中国生物学文摘2006，20，5： | 16 |
| 12 | Efficacy of postoperative antiviral combined transcatheter arterial chemoembolization therapy in prevention of hepatitis B-related hepatocellular carcinoma recurrence显示文摘 | YAN Qiang NI Jun ZHANG Guo-lei YAO Xing YUAN Wen-bin ZHOU Lin ZHENG Shu-sen | 2013 | Chinese Medical Journal2013，，5： | 16 |
| 13 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 14 | OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models显示文摘AIM: To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique. METHODS: Using OB glue paste technique, orthtopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed. RESULTS: The success rates of orthotopic transplan- tation of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%. CONCLUSION: OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors. | Jun Shi Pin-Kang Wei Shen Zhang Zhi-Feng Qin Jun Li Da-Zhi Sun Yan Xiao Zhi-Hong Yu Hui-Ming Lin Guo-Jing Zheng Xiao-Mei Su Ya-Lin Chen Yan-Fang Liu Ling Xu | 2008 | World Journal of Gastroenterology2008，14，30： | 15 |
| 15 | Reduced Expression of Programmed Cell Death 5 Protein in Tissue of Human Prostate Cancer显示文摘Objective To investigate the expression of programmed cell death 5(PDCD5) in tissues of normal human prostate(NP),benign prostatic hyperplasia(BPH),and prostate cancer(PCa) in order to assess the clinical role of PDCD5 in PCa.Methods PDCD5 expression was determined by EnVision immunohistochemical staining in formalin-fixed and paraffin-embedded specimens obtained from 12 subjects with NP,22 with BPH,and 22 with PCa.In addition,PCa cases were classified as low/middle-risk(Gleason sum≤7) and high-risk(Gleason sum>7) on the basis of Gleason grade.Positive expression rates and intensity of PDCD5 protein were observed under light microscope and analyzed with computer imaging technique.Expression of PDCD5 was compared among different prostatic tissues.Results The expression of PDCD5 was significantly lower in tissue of PCa than in tissues of NP and BPH(P<0.01).However,there was no significant difference in PDCD5 expression between tissues of NP and BPH.In addition,the expression of PDCD5 was further downregulated with the increase of Gleason sum in PCa.Conclusions By downregulating apoptosis,low PDCD5 expression may play an important role in the occurrence and development of PCa.PDCD5 is supposed to have a potential clinical value to be a new predictor of progression and target of gene therapy in PCa. | Yue-jun Du Lin Xiong Yan Lou Wan-long Tan Shao-bin Zheng | 2009 | Chinese Medical Sciences Journal2009，24，4： | 15 |
| 16 | Effects of viral infection and microbial diversity on patients with sepsis:A retrospective study based on metagenomic next-generation sequencing显示文摘BACKGROUND: The study aims to investigate the performance of a metagenomic next-generationsequencing (NGS)-based diagnostic technique for the identifi cation of potential bacterial and viral infectionsand eff ects of concomitant viral infection on the survival rate of intensive care unit (ICU) sepsis patients.METHODS: A total of 74 ICU patients with sepsis who were admitted to our institution from February1, 2018 to June 30, 2019 were enrolled. Separate blood samples were collected from patients for bloodcultures and metagenomic NGS when the patients’ body temperature was higher than 38 °C. Patients’demographic data, including gender, age, ICU duration, ICU scores, and laboratory results, were recorded.The correlations between pathogen types and sepsis severity and survival rate were evaluated.RESULTS: NGS produced higher positive results (105 of 118;88.98%) than blood cultures(18 of 118;15.25%) over the whole study period. Concomitant viral infection correlated closelywith sepsis severity and had the negative effect on the survival of patients with sepsis. However,correlation analysis indicated that the bacterial variety did not correlate with the severity of sepsis.CONCLUSIONS: Concurrent viral load correlates closely with the severity of sepsis and thesurvival rate of the ICU sepsis patients. This suggests that prophylactic administration of antiviraldrugs combined with antibiotics may be benefi cial to ICU sepsis patients. | Li-wei Duan Jin-long Qu Jian Wan Yong-hua Xu Yi Shan Li-xue Wu Jin-hao Zheng Wei-wei Jiang Qi-tong Chen Yan Zhu Jian Zhou Wen-bo Yu Lei Pei Xi Song Wen-fang Li Zhao-fen Lin | 2021 | World Journal of Emergency Medicine2021，12，1： | 14 |
| 17 | 新疆传统发酵奶酪中乳酸菌的益生特性研究(英文)显示文摘目的:分离鉴定新疆牧民家庭自制的4份奶酪样品中的6株乳酸菌,研究其益生特性。创新点:通过对新疆传统发酵乳制品中乳酸菌的分离及其抗氧化和降脂特性的测定,筛选到益生特性良好同时也能延长模式生物秀丽线虫寿命的菌株。方法:对分离自传统发酵乳制品中的乳酸菌进行生理生化分析和16S rDNA分子生物学鉴定,筛选出6株乳酸菌,并测定其耐酸、耐胆盐、抑菌、亲水性和共聚集能力;测定其抗氧化、降解胆固醇和甘油三酯的能力;测定乳酸菌对秀丽线虫寿命的影响。结论:实验表明,该6株菌具有较好的耐酸性,在胆盐环境中也有较好的存活率。同时,这6株菌均有清除自由基和降解脂肪的能力。此外,其中3株具有延长秀丽线虫寿命的能力。综上所述,这6株菌具有较好的益生特性,可作为优良的潜在益生菌株。 | Ramila AZAT Yan LIU Wei LI Abdurihim KAYIR Ding-bo LIN Wen-wen ZHOU Xiao-dong ZHENG | 2016 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2016，17，8： | 13 |
| 18 | A study on arsenic trioxide inducing in vitro apoptosis of gastric cancer cell lines显示文摘INTRODUCTION Cell apoptosis,which involves the biologic regulation of the numbers and vital activity of cells,is an important metaboloc process in both normal cells and tumor cells. | Qin Long Gu Ning Li Li Zheng Gang Zhu Hao Ran Yin Yan Zhen Lin | 2000 | World Journal of Gastroenterology2000，6，3： | 12 |
| 19 | A modified protocol with rituximab and intravenous immunoglobulin in emergent ABO-incompatible liver transplantation for acute liver failure显示文摘BACKGROUND: The established procedure for ABO-incompatible liver transplantation(ABO-I LT) was too complicated to be used in case of emergency. We developed a protocol consisting of rituximab and intravenous immunoglobulin(IVIG) for ABO-I LT in patients with acute liver failure(ALF).METHODS: The data from 101 patients who had undergone liver transplantation(LT) for ALF were retrospectively analyzed.The patients were divided into two groups: ABO-compatible liver transplantation group(ABO-C LT, n=66) and ABO-I LT group(n=35). All the patients in the ABO-I LT group received a single dose of rituximab(375 mg/m2) and IVIG(0.4 g/kg per day) at the beginning of the operation. IVIG was administered for 10 consecutive days after LT. Plasma exchange, splenectomy and graft local infusion were omitted in the protocol.Quadruple immunosuppressive therapy including basiliximab,corticosteroids, tacrolimus and mycophenolatemofetil was used to reinforce immunosuppression.RESULTS: The 3-year cumulative patient survival rates in the ABO-I LT and ABO-C LT groups were 83.1% and 86.3%,respectively(P>0.05), and the graft survival rates were 80.0%and 86.3%, respectively(P>0.05). Two patients(5.7%) suffered from antibody-mediated rejection in the ABO-I LT group.Other complications such as acute cellular rejection, biliary complication and infection displayed no significant differences between the two groups.CONCLUSIONS: The simplified treatment consisting of rituximab and IVIG prevented antibody-mediated rejection for LT of blood-type incompatible patients. With this treatment, the patients did not need plasma exchange, splenectomy and graft local infusion. This treatment was safe and efficient for LT of the patients with ALF. | Tian Shen Bing-Yi Lin Jun-Jun Jia Zhuo-Yi Wang Li Wang Qi Ling Lei Geng Sheng Yan Shu-Sen Zheng | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，4： | 11 |
| 20 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
您所在的机构暂未开通此部分的功能，请联系管理员