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| 1 | Breast magnetic resonance imaging in patients with occult breast carcinoma: evaluation on feasibility and correlation with histopathological findings显示文摘是的背景一个不平常的演讲,秘密主要乳癌在临床的实践仍然是诊断、治疗学的挑战。这研究试图回顾地估计胸的可行性在有恶意的腋的 lymphadenopathy 和未知主要恶意的病人的磁性的回声成像(MRI ) ,和与有秘密的胸癌的 35 个女人全部的组织病理学说的 characteristics.Methods A 的关联与动态提高对比的胸 MRI 被评估。完全使连续节在所有情况中被使用。MRI 性能被估计并且与 35 个病人中的 21 个被发现组织学地有主要的胸癌的组织病理学说的 findings.Results 相关。21 个病人中的二十个有反常先生调查结果, 1 个病人让正常 MRI 学习。留下 14 个病人, 10 在 MRI 和外科上是否定的。MRI 和没有相应肿瘤上的四有的可疑改进被发现。在 45% 的改进在 55% 被发现的有质量的损害(11/20 ) 和 ductual 和部分改进。主要肿瘤的平均直径是 15 公里。侵略 ductal 癌在 81% 被发现(17/21 ) 。17 侵略 ductual 癌之一太小,不能被分级。留下中的十四个 16 作为等级 II 被分类并且 2 是等级我。35 个病人中的 32 个收到了雌激素受体,孕酮受体和人表皮的生长因素受体 2 考试并且 32 中的 12 个是有小尺寸的三元组否定的胸 carcinoma.Conclusions 团损害,有 ductal 或片断改进的损害是在有秘密乳癌的病人的普通 MRI 特征。主要肿瘤的主导的类型是有中等组织病理学说的等级的侵略 ductal 癌。三元组否定的胸癌的率可能在秘密乳癌是更高的。 | LU Hong XU Yi-lin ZHANG Shu-ping LANG Rong-gang Chi S. Zee LIU Pei-fang FU Li | 2011 | Chinese Medical Journal2011，，12： | 31 |
| 2 | Astragalus polysaccharide stimulates glucose uptake in L6 myotubes through AMPK activation and AS160/TBCID4 phosphorylation显示文摘 | Jian LIU Jing-fang ZHANG Jin-zhi LU De-ling ZHANG Ke LI Ke SU Jing WANG Ye-min ZHANG Nian WANG Si-tu YANG Lang BU Jing-ping OU-YANG | 2013 | Acta Pharmacologica Sinica2013，34，1： | 26 |
| 3 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 4 | Numerical Prediction and Performance Experiment in a Deep-well Centrifugal Pump with Different Impeller Outlet Width显示文摘The existing research of the deep-well centrifugal pump mainly focuses on reduce the manufacturing cost and improve the pump performance,and how to combine above two aspects together is the most difficult and important topic.In this study,the performances of the deep-well centrifugal pump with four different impeller outlet widths are studied by the numerical,theoretical and experimental methods in this paper.Two stages deep-well centrifugal pump equipped with different impellers are simulated employing the commercial CFD software to solve the Navier-Stokes equations for three-dimensional incompressible steady flow.The sensitivity analyses of the grid size and turbulence model have been performed to improve numerical accuracy.The flow field distributions are acquired and compared under the design operating conditions,including the static pressure,turbulence kinetic energy and velocity.The prototype is manufactured and tested to certify the numerical predicted performance.The numerical results of pump performance are higher than the test results,but their change trends have an acceptable agreement with each other.The performance results indicted that the oversize impeller outlet width leads to poor pump performances and increasing shaft power.Changing the performance of deep-well centrifugal pump by alter impeller outlet width is practicable and convenient,which is worth popularizing in the engineering application.The proposed research enhances the theoretical basis of pump design to improve the performance and reduce the manufacturing cost of deep-well centrifugal pump. | SHI Weidong ZHOU Ling LU Weigang PEI Bing LANG Tao | 2013 | Chinese Journal of Mechanical Engineering2013，26，1： | 20 |
| 5 | Association between the level of CD4^+T lymphocyte microRNA-155 and coronary artery disease in patients with unstable angina pectoris显示文摘Objective To study the association between the expression of microRNA-155(miRNA-155)in peripheral blood CD4^+T lymphocytes and the level of semrn interferon-7(IFN-7)concentration and the severity of coronary artery disease (CAD).Methods After coronary angiography,252patients with suspected unstable angina pectoris (UAP)were divided into the UAP group (128patients with CAD confirmed by angiography)and the control group (124patients without CAD confirmed by angiography).Fresh peripheral blood was extracted 16-24h before coronary angiography,CD4^+T lymphocytes was tested using immunomagnetic beads,the expression ofmiRNA-155was tested using quantitative PCR and the expression of IFN-7was tested using enzyme-linked immunosorbent assay (ELISA).According to the results of angiography,Gensini score of coronary artery lesions was analyzed.Furthermore,we also analysis the association between the level of miRNA-155in peripheral blood CD4^+T lymphocytes,the level of serum IFN-γand Gensini score of coronary lesion.Results The levels ofmiRNA-155(0.49±0.08vs.0.23±0.09)and IFN-7(227.58±26.01vs.141.23±17.89)in the UAP group were significantly higher than that of the control group,the difference was statistically significant.The level of miRNA-155and IFN-γwere positively correlated with Gensini score of CAD (r =0.534,r =0.713,respectively,all P <0.05).The level of miRNA-155was positively correlated with the level of IFN-γ,(r =0.686,P <0.05).Conclusions The level of miRNA-155in peripheral blood CD4^+T lymphocytes and the level of IFN-γ are closely correlated with the severity of CAD. | Zi-Liang WE Hai-Li LU Qiang SU Lang Li | 2018 | Journal of Geriatric Cardiology2018，15，10： | 16 |
| 6 | Mushroom Poisoning Outbreaks-China,2020显示文摘What is already known about this topic?Acute liver failure,rhabdomyolysis,acute renal failure,and hemolysis caused by poisonous mushrooms are the most important mushroom poisoning threats to the Chinese population.The most notorious lethal mushrooms are the species from genera Amanita,Lepiota,and Galerina that cause acute liver failure,and Russula subnigricans that leads to rhabdomyolysis.What is added by this report?In 2020,the total number of investigations reached 676,involving an estimated 102 species of poisonous mushrooms,24 of which were newly recorded in China.Gyromitra venenata was newly discovered in incidents in Yunnan and Guizhou provinces and were the first reported poisonings due to gyromitrins in China since 2000.The rare poisoning Shiitake mushroom dermatitis was recorded in China.Hemolysis poisoning caused by Paxillus involutus was recorded for the second time since the beginning of the new century,resulting in one death in Inner Mongolia Autonomous Region.What are the implications for public health practice?Promoting knowledge about safe consumption of mushrooms is essential to reduce mushroom poisonings.It is not wise to collect and eat wild mushrooms.For southwestern provinces such as Yunnan,especially,caution must be exercised with unfamiliar mushroom species. | Haijiao Li Hongshun Zhang Yizhe Zhang Jing Zhou Yu Yin Qian He Shaofeng Jiang Peibin Ma Yutao Zhang Ke Wen Yuan Yuan Nan Lang Bowen Cheng Junjia Lu Chengye Sun | 2021 | China CDC weekly2021，3，3： | 13 |
| 7 | Study on Random Initiation Phenomenon for Sympathetic Detonation of Explosive显示文摘It is important to understand the characteristics of explosive sympathetic detonation for explosive safety.Sympathetic reaction test of GHL(RDX/Al/Binder)explosive charges with shell are conducted.A model of the sympathetic reaction test is established.The elements-apart method and nodes random-failure method are used in the model to describe the expansion progress of shell expanding and the randomly forming process of fragments.Random detonation phenomena of acceptor charge are simulated. | Lang CHEN Chen WANG Chang-gen FENG Feng LU Jian-ying LU Xiao-feng WANG Xin GUO | 2013 | Defence Technology(防务技术)2013，9，4： | 12 |
| 8 | Mushroom Poisoning Outbreaks--China,2019显示文摘What is already known about this topic?Mushroom poisoning is becoming one of the most serious food safety issues in China,which is responsible for nearly a half of all oral poisoning deaths.What is added by this report?In China,many mushrooms were previously“recorded”as poisonous.In this study,about 70 species obtained from mushroom poisoning incidents including several new records were confirmed accurately by morphological and molecular evidence in 2019,and spatial and temporal distribution characters of 13 lethal mushrooms were summarized systematically.What are the implications for public health practice?Precise and timely species identification is of pivotal importance in mushroom incidents.More efforts and cooperation are continued to be needed urgently for the governments,CDC staff,doctors and mycologists in future. | Haijiao Li Hongshun Zhang Yizhe Zhang Kaiping Zhang Jing Zhou Yu Yin Shaofeng Jiang Peibin Ma Qian He Yutao Zhang Ke Wen Yuan Yuan Nan Lang Junjia Lu Chengye Sun | 2020 | China CDC weekly2020，2，2： | 11 |
| 9 | Chinese expert consensus on the non-invasive imaging examination pathways of stable coronary artery disease显示文摘 | Yun-Dai CHEN Wei-Yi FANG Ji-Yan CHEN Zhan-Ming FAN Chuan-Yu GAO Jun-Bo GE Zuo-Xiang HE Yong HUO Lang LI Si-Jin LI Xi-Lie LU Bin LV Ju-Ying QIAN Ya-Jun SHI Zhu-Jun SHEN Jing WANG Yi-Ning WANG Lei XU Li YANG Bo YU Mei ZHANG Jun-Jie YANG Shu-Yang ZHANG Xiao-Li ZHANG Shi-Hua ZHAO Yang ZHENG Yu-Chi HAN Guang ZHI | 2018 | Journal of Geriatric Cardiology2018，15，1： | 10 |
| 10 | Proportion of Uterine Malignant Tumors in Patients with Laparoscopic Myomectomy: A National Multicenter Study in China显示文摘 | Hua Yang Xiao-Chuan Li Chen Yao Jing-He Lang Hang-Mei Jin Ming-Rong Xi Gang Wang Lu-Wen Wang Min Hao Yan Ding Jie Chen Jian-Qing Zhang Lu Han Cheng-Xiu Guo Xiang Xue Yan Li Jian-Hua Zheng Man-Hua Cui Huai-Fang Li Guang-Shi Tao Long Chen Su-Min Wang An-Wei LU Ze-Hua Huang Qing Liu Ya-Li Zhuang Xiang-Hua Huang Gen-Hai Zhu Ou-Ping Huang Li-Na Hu Mu-Jun Li Hong-Lin Zhou Jing-Hui Song Lan Zhu | 2017 | Chinese Medical Journal2017，，22： | 10 |
| 11 | 雷帕霉素对小鼠百草枯中毒导致肺纤维化的治疗作用(英文)显示文摘目的:评估雷帕霉素(RAPA)对小鼠百草枯(PQ)中毒导致肺纤维化的治疗作用,并初步探讨其作用机制。创新点:首次在小鼠PQ中毒的模型中证明RAPA可明显抑制肺纤维化,且此作用与转化生长因子-β1(TGF-β1)的抑制相关。方法:将C57BL/6J雄鼠分为对照组(腹腔注射生理盐水)和实验组(腹腔注射10 mg/kg PQ)。实验组根据采用的治疗不同,可分为以下四组:PQ组、PQ+RAPA组、PQ+MP(甲强龙)组和PQ+MP+RAPA组。用苏木精-伊红染色法(H&E)和马松(Masson)三色染色法观察肺组织病理结构变化,用酶联免疫吸附测定(ELISA)试剂盒检测肺组织中的羟脯氨酸(HYP)含量,用免疫组化和免疫印迹的方法检测TGF-β1和α-平滑肌肌动蛋白(α-SMA)的表达水平。结论:本实验中小鼠的肺组织病理染色结果显示,RAPA治疗能缓解肺纤维化导致的病理改变(图3)。ELISA实验结果显示,RAPA治疗28天后可显著降低肺组织中HYP的含量(图4)。免疫组化和免疫印迹实验结果显示,RAPA治疗能明显下调肺组织中TGF-β1和α-SMA的高表达(图5和6)。综上所述,RAPA在治疗PQ中毒导致的肺纤维化中有重要价值。 | Xue SHAO Meng LI Chong LUO Ying-ying WANG Ying-ying LU Shi FENG Heng LI Xia-bing LANG Yu-cheng WANG Chuan LIN Xiu-jin SHEN Qin ZHOU Hong JIANG Jiang-hua CHEN | 2015 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2015，16，1： | 7 |
| 12 | A 14-year multi-institutional collaborative study of Chinese pelvic floor surgical procedures related to pelvic organ prolapse显示文摘Background:It has been a global trend that increasing complications related to pelvic floor surgeries have been reported over time.The current study aimed to outline the development of Chinese pelvic floor surgeries related to pelvic organ prolapse(POP)over the past 14 years and investigate the potential influence of enhanced monitoring conducted by the Chinese Association of Urogynecology since 2011.Methods:A total of 44,594 women with POP who underwent pelvic floor surgeries between October 1,2004 and September 30,2018 were included from 22 tertiary academic medical centers.The data were reported voluntarily and obtained from a database.We compared the proportion of each procedure in the 7 years before and 7 years after September 30,2011.The data were analyzed by performing Z test(one-sided).Results:The number of different procedures during October 1,2011-September 30,2018 was more than twice that during October 1,2004-September 30,2011.Regarding pelvic floor surgeries related to POP,the rate of synthetic mesh procedures increased from 38.1%(5298/13,906)during October 1,2004-September 30,2011 to 46.0%(14,107/30,688)during October 1,2011-September 30,2018,whereas the rate of non-mesh procedures decreased from 61.9%(8608/13,906)to 54.0%(16,581/30,688)(Z=15.53,P<0.001).Regarding synthetic mesh surgeries related to POP,the rates of transvaginal placement of surgical mesh(TVM)procedures decreased from 94.1%(4983/5298)to 82.2%(11,603/14,107)(Z=20.79,P<0.001),but the rate of laparoscopic sacrocolpopexy(LSC)procedures increased from 5.9%(315/5298)to 17.8%(2504/14,107).Conclusions:The rate of synthetic mesh procedures increased while that of non-mesh procedures decreased significantly.The rate of TVM procedures decreased while the rate of LSC procedures increased significantly.Trial registration number:NCT03620565,http://gffzz111276e5ed644ca6squxwunuwukqk66co.ffgz.tsg.suse.edu.cn. | Zhi-Jing Sun Xiu-Qi Wang Jing-He Lang Tao Xu Yong-Xian Lu Ke-Qin Hua Jin-Song Han Huai-Fang Li Xiao-Wen Tong Ping Wang Jian-Liu Wang Xin Yang Xiang-Hua Huang Pei-Shu Liu Yan-Feng Song Hang-Mei Jin Jing-Yan Xie Lu-Wen Wang Qing-Kai Wu Jian Gong Yan Wang Li-Qun Wang Zhao-Ai Li Hui-Cheng Xu Zhi-Jun Xia Li-Na Gu Qing Liu Lan Zhu | 2021 | Chinese Medical Journal2021，，2： | 7 |
| 13 | Experimental and numerical study on fretting wear and fatigue of full-scale railway axles显示文摘This study investigated the fretting wear and fatigue of full-scale railway axles.Fatigue tests were conducted on full-scale railway axles,and the fretting wear and fretting fatigue in the fretted zone of the railway axles were analysed.Three-dimensional finite element models were established based on the experimental results.Then,multi-axial fatigue parameters and a linear elastic fracture mechanics-based approach were used to investigate the fretting fatigue crack initiation and propagation,respectively,in which the role of the fretting wear was taken into account.The experimental and simulated results showed that the fretted zone could be divided into zones I-III according to the surface damage morphologies.Fretting wear alleviated the stress concentration near the wheel seat edge and resulted in a new stress concentration near the worn/unworn boundary in zone II,which greatly promoted the fretting crack initiation at the inner side of the fretted zone.Meanwhile,the stress concentration also increased the equivalent stress intensity factor range DKeq below the mating surface,and thus promoted the propagation of fretting fatigue crack.Based on these findings,the effect of the stress redistribution resulting from fretting wear is suggested to be taken into account when evaluating the fretting fatigue in railway axles. | Lang Zou Dongfang Zeng Yabo Li Kai Yang Liantao Lu Caiqin Yuan | 2020 | Railway Engineering Science2020，28，4： | 7 |
| 14 | Intercropping of rice varieties increases the eff iciency of blast control through reduced disease occurrence and variability显示文摘Creating a crop-heterogeneous system by intraspecific mixtures of different rice varieties can substantially reduce blast diseases.Such variety mixtures provide an ecological approach for effective disease control,maintaining high yields with the minimum fungicide applications.Whether such an approach is universally applicable for random rice variety combinations and what is the variation pattern of the diseases under intercropping still remains unclear.We conducted two-year large-scale field experiments involving 47 rice varieties/lines and 98 variety-combinations to compare the occurrence of rice blast in monoculture and intercropping plots at multiple sites.In the experiments,the plant height of the selected traditional varieties was about 30 cm taller,and their life cycle was 10 days longer,than that of the improved rice varieties.The monoculture included either traditional or modern rice varieties grown in separate plots.The intercropping included both traditional and modern rice varieties planted together in the same plots.Results from the field experiments under natural disease conditions demonstrated significant reduction for rice blast disease in intercropping plots,compared with that in monoculture plots.For traditional varieties,the average blast incidence reduced from^26%in monoculture to^10%in intercropping,and the disease severity reduced from^17 in monoculture to^5 in intercropping.For modern varieties,the average blast incidence reduced from^19%in monoculture to^10%in intercropping,and the severity from^10 in monoculture to^4 in intercropping.Traditional rice varieties(~72%)had a much greater increase in the efficiency of disease control than modern varieties(~60%).In addition,substantially lower values of variance in the blast incidence and severity was detected among the variety combinations in intercropping plots than in monoculture plots.Based on these results,we conclude that the intercropping or mixture of rice varieties greatly reduces the occurrence and variation of rice blast disease in particular variety combinations,which makes the intercropping system more stable and consistent for disease suppression on a large scale of rice cultivation. | HAN Guang-yu LANG Jie SUN Yan WANG Yun-yue ZHU You-yong LU Bao-rong | 2016 | Journal of Integrative Agriculture2016，15，4： | 6 |
| 15 | 温带草原退化后土壤微生物群落结构和功能的变化显示文摘草原退化是草原生产力维持面临的一个重大挑战,这一过程显著影响着草原生态系统的能量流动和土壤养分变化过程,进而直接或间接地影响着土壤微生物。我们的研究目的首先是调查不同草原退化程度(即未退化、中度退化和严重退化)如何影响着内蒙古温带草原的土壤微生物组成、多样性和功能,其次是阐明哪些生物和非生物因素导致了这些变化。我们的研究主要通过高通量测序技术分析土壤微生物的群落组成,并且采用FAPROTAX工具和FUNGuild工具分别预测细菌群落和真菌群落的功能。研究发现:草原退化显著降低了土壤细菌的多样性,但对真菌多样性影响不大。地下生物量、土壤有机碳和总氮与细菌的多样性变化呈显著正相关关系。草原退化显著增加了绿弯菌门的相对丰度(由2.48%提高到8.40%),降低了厚壁菌门的相对丰度(由3.62%降低到1.08%)。其次,草原退化也显著增加了球囊菌门的相对丰度(从0.17%提高到1.53%),降低了担子菌门的相对丰度(从19.30%降低到4.83%)。致病菌的相对丰度在草原退化过程中显著下降。此外,草原退化对土壤细菌群落的功能有显著的影响,尤其是与土壤碳氮循环相关的土壤细菌群落。我们的结果表明,土壤细菌群落对草原退化的响应比真菌群落更敏感。 | Yang Yu Lang Zheng Yijun Zhou Weiguo Sang Jianing Zhao Lu Liu Chao Li Chunwang Xiao | 2021 | Journal of Plant Ecology2021，14，3： | 5 |
| 16 | Expression of p53 in myocardium following coronary microembolization inrats and its significance显示文摘Background Cardiomyocyte apoptosis is a primary cause for coronary microembolization (CME)-induced cardiac dysfunction, p53induces cell growth retardation and apoptosis through stress pathway. The present study investigated the mechanism of p53-induced myocar-dial apoptosis and cardiac dysfunction by activating the mitochondrion apoptotic pathway following CME. Methods Forty SD rats wereequally divided into microembolization (CME), sham operation (sham), CME+siRNA-p53, and CME+control-p53 groups. The CME ratmodel was established by injecting microembolization spheres via the left ventricle. Cardiac ultrasound, TUNEL, fluorescence quantitativePCR, and Western blot were used to assess the cardiac function indicators, cardiomyocyte apoptosis, and the expressions of mRNA and pro-tein in myocardial tissues, respectively. Results Echocardiography revealed a significantly reduced cardiac function of the CME group thanthe sham group while the CME-induced cardiac dysfunction was improved in the CME+siRNA-p53 group. The indicators of myocardialapoptosis in the CME group increased significantly than the sham group; those of the CME+siRNA-p53 group decreased significantly thanthe CME group. Fluorescence quantitative PCR and Western blot demonstrated that p53, Bbc3 (PUMA), and cleaved caspase-3 expressionswere significantly increased, and BCL-2 expression was declined in myocardial tissues of the CME group compared to the sham group. Acontrasting result was observed in the CME+siRNA-p53 group as compared to the CME group. Conclusions P53 is involved in theCME-induced cardiac dysfunction, which may up-regulate Bbc3 to activate BCL-2/caspase3 mitochondrial apoptotic pathway and inducemyocardial apoptosis. Inhibiting the p53 expression can effectively suppress this pathway, thereby reducing myocardial apoptosis and car-diac dysftmction. | Yu-Han SUN Qiang SU Lang LI Xian-Tao WANG Yuan-Xi LU Jia-Bao LIANG | 2017 | Journal of Geriatric Cardiology2017，14，5： | 5 |
| 17 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 18 | Effect of toll-like receptor 3 agonist poly I:C on intestinal mucosa and epithelial barrier function in mouse models of acute colitis显示文摘AIM To investigate potential effects of poly I:C on mucosal injury and epithelial barrier disruption in dextran sulfate sodium(DSS)-induced acute colitis.METHODS Thirty C57BL/6 mice were given either regular drinking water(control group) or 2%(w/v) DSS drinking water(model and poly I:C groups) ad libitum for 7 d. Poly I:C was administrated subcutaneously (20 μg/mouse) 2 h prior to DSS induction in mice of the poly I:C group. Severity of colitis was evaluated by disease activity index, body weight, colon length, histology and myeloperoxidase (MPO) activity, as well as the production of proinflammatory cytokines, including tumor necrosis factor-α(TNF-α), interleukin 17(IL-17) and interferon-γ (IFN-γ). Intestinal permeability was analyzed by the fluorescein isothiocyanate labeleddextran (FITC-D) method. Ultrastructural features of the colon tissue were observed under electron microscopy. Expressions of tight junction(TJ) proteins, including zo-1, occludin and claudin-1, were measured by immunohistochemistry/immunofluorescence, Western blot and real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS DSS caused significant damage to the colon tissue in the model group. Administration of poly I:C dramatically protected against DSS-induced colitis, as demonstrated by less body weight loss, lower disease activity index score, longer colon length, colonic MPO activity, and improved macroscopic and histological scores. It also ameliorated DSS-induced ultrastructural changes of the colon epithelium, as observed under scanning electron microscopy, as well as FITC-D permeability. The m RNA and protein expressions of TJ protein, zo-1, occludin and claudin-1 were also found to be significantly enhanced in the poly I:C group, as determined by immunohistochemistry/immunofluorescence, Western blot and RT-q PCR. By contrast, poly I:C pretreatment markedly reversed the DSS-induced up-regulated expressions of the inflammatory cytokines TNF-α, IL-17 and IFN-γ.CONCLUSION Our study suggested that poly I:C may protect against DSS-induced colitis through maintaining integrity of the epithelial barrier and regulating innate immune responses, which may shed light on the therapeutic potential of poly I:C in human colitis. | Hong-Wei Zhao Yue-Hong Yue Hua Han Xiu-Li Chen Yong-Gang Lu Ji-Min Zheng Hong-Tao Hou Xiao-Meng Lang Li-Li He Qi-Lu Hu Zi-Qian Dun | 2017 | World Journal of Gastroenterology2017，23，6： | 5 |
| 19 | Application of Percutaneous Balloon Mitral Valvuloplasty in Patients of Rheumatic Heart Disease Mitral Stenosis Combined with Tricuspid Regurgitation显示文摘 | Zhang-Qiang Chen Lang Hong Hong Wang Lin-Xiang Lu Qiu-Lin Yin Heng-Li Lai Hua-Tai Li Xiang Wang | 2015 | Chinese Medical Journal2015，，11： | 5 |
| 20 | 3D printing of high-performance micro-supercapacitors with patterned exfoliated graphene/carbon nanotube/silver nanowire electrodes显示文摘Micro-supercapacitors(MSCs)show great potential as on-chip energy storage devices for portable electronics.The major flaw of thin-film MSCs is their low energy density.To improve the energy density,thicker electrodes are required.However,the fabrication of MSCs with thick electrodes remains a challenge.In this work,a novel 3D printing method is employed to fabricate high-performance MSCs with interdigitated exfoliated graphene(EG)/carbon nanotube(CNT)/silver nanowire(Ag NW)electrodes.The nanowelding of Ag NW junction plays a critical role in the realization of 3D printing.To enhance the electrochemical performances of EG,phosphorus atoms are incorporated into the carbon framework with 1.7 at%.The areal capacitance of the 3D printed MSC is 21.6 mF cm^(-2)at a scan rate of 0.01 V s^(-1).The areal energy density of the MSC ranges from 0.5 to 2μWh cm^(-2)with a maximum power density of 2.5 mW cm^(-2). | LIU Lang LU JunYong LONG XinLin ZHOU Ren LIU YingQuan WU YiTing YAN KangWei | 2021 | Science China(Technological Sciences)2021，64，5： | 4 |
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