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| 1 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 2 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 3 | Advances in phase-sensitive optical time-domain reflectometry显示文摘Phase-sensitive optical time-domain reflectometry(Φ-OTDR)has attracted numerous attention due to its superior performance in detecting the weak perturbations along the fiber.Relying on the ultra-sensitivity of light phase to the tiny deformation of optical fiber,Φ-OTDR has been treated as a powerful technique with a wide range of applications.It is fundamental to extract the phase of scattering light wave accurately and the methods include coherent detection,I/Q demodulation,3 by 3 coupler,dual probe pulses,and so on.Meanwhile,researchers have also made great efforts to improve the performance ofΦ-OTDR.The frequency response range,the measurement accuracy,the sensing distance,the spatial resolution,and the accuracy of event discrimination,all have been enhanced by various techniques.Furthermore,lots of researches on the applications in various kinds of fields have been carried out,where certain modifications and techniques have been developed.Therefore,Φ-OTDR remains as a booming technique in both researches and applications. | Shuaiqi Liu Feihong Yu Rui Hong Weijie Xu Liyang Shao Feng Wang | 2022 | Opto-Electronic Advances2022，5，3： | 7 |
| 4 | Secalonic acid D induces cell apoptosis in both sensitive and ABCG2-overexpressing multidrug resistant cancer cells through upregulating c-Jun expression显示文摘Secalonic acid D(SAD) could inhibit cell growth in not only sensitive cells but also multidrug resistant(MDR) cells. However, the molecular mechanisms need to be elucidated. Here, we identified that SAD possessed potent cytotoxicity in 3 pairs of MDR and their parental sensitive cells including S1-MI-80 and S1,H460/MX20 and H460, MCF-7/ADR and MCF-7 cells. Furthermore, SAD induced cell G2/M phase arrest via the downregulation of cyclin B1 and the increase of CDC2 phosphorylation. Importantly, JNK pathway upregulated the expression of c-Jun in protein level and increased c-Jun phosphorylation induced by SAD, which was linked to cell apoptosis via c-Jun/Src/STAT3 pathway. To investigate the mechanisms of upregulation of c-Jun protein by SAD, the mR NA expression level and degradation of c-Jun were examined. We found that SAD did not alter the mR NA level of c-Jun but inhibited its proteasome-dependent degradation. Taken together, these results implicate that SAD induces cancer cell death through c-Jun/Src/STAT3 signaling axis by inhibiting the proteasome-dependent degradation of c-Jun in both sensitive cells and ATP-binding cassette transporter sub-family G member 2(ABCG2)-mediated MDR cells. | Hong Zhang Liyan Huang Liyang Tao Jianye Zhang Fang Wang Xu Zhang Liwu Fu | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 5 |
| 5 | A Self-organizing Bearings-only Target Tracking Algorithm in Wireless Sensor Network显示文摘在纯方位目标跟踪中,无线传感器网络根据各个节点测量的目标方位使用适应性滤波器跟踪目标。网络需要高性能的管理算法从而达到定位精度和能量节约两个方面的高性能水平。本文提出了一种网络目标跟踪算法。该算法在每个采样时刻通过自组织原理选择定位性能最好的一组节点测量目标。与传统的全局式节点选择算法相比,该算法避免了网络需要掌握全局节点位置的问题。依据该算法,每个节点判断自己是否工作仅仅根据它自己的位置信息和简单的数学计算。仿真结果证明这种算法与执行全局遍历的节点选择的算法具有相近的定位精度。而且,它具有很好的鲁棒性能和可扩展性。由于每个采样时刻只有一部分节点参与算法的执行,它的能量代价也是有限的。 | Xu Zhen Rui Liyang | 2009 | Chinese Journal of Aeronautics2009，22，6： | 5 |
| 6 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 7 | Spatial modulation of nanopattern dimensions by combining interference lithography and grayscale-patterned secondary exposure显示文摘Functional nanostructures are exploited for a variety of cutting-edge fields including plasmonics,metasurfaces,and biosensors,just to name a few.Some applications require nanostructures with uniform feature sizes while others rely on spatially varying morphologies.However,fine manipulation of the feature size over a large area remains a substantial challenge because mainstream approaches to precise nanopatterning are based on low-throughput pixel-by-pixel processing,such as those utilizing focused beams of photons,electrons,or ions.In this work,we provide a solution toward wafer-scale,arbitrary modulation of feature size distribution by introducing a lithographic portfolio combining interference lithography(IL)and grayscale-patterned secondary exposure(SE).Employed after the high-throughput IL,a SE with patterned intensity distribution spatially modulates the dimensions of photoresist nanostructures.Based on this approach,we successfully fabricated 4-inch wafer-scale nanogratings with uniform linewidths of<5%variation,using grayscale-patterned SE to compensate for the linewidth difference caused by the Gaussian distribution of the laser beams in the IL.Besides,we also demonstrated a wafer-scale structural color painting by spatially modulating the filling ratio to achieve gradient grayscale color using SE. | Zhuofei Gan Hongtao Feng Liyang Chen Siyi Min Chuwei Liang Menghong Xu Zijie Jiang Zhao Sun Chuying Sun Dehu Cui Wen-Di Li | 2022 | Light(Science & Applications)2022，11，5： | 3 |
| 8 | Recent Advances in Wide Bandgap Polymer Donors and Their Applications in Organic Solar Cells显示文摘Organic solar cells(OSCs)have attracted wide research interests in the past decades.In recent years,our group mainly focused on the new photovoltaic materials design and device engineering technologies for highly efficient OSCs.This account mainly summarizes our recent studies on the structural design of wide bandgap(WBG)polymers and their morphology control as well as applications in OSCs.Additionally,we introduce our work on binary and ternary blend devices and tandem solar cells containing these materials. | Xiaopeng Xu Liyang Yu Qiang Peng | 2021 | Chinese Journal of Chemistry2021，39，2： | 3 |
| 9 | Total ionizing radiation effects of 2-T SONOS for 130 nm/4 Mb NOR flash memory technology显示文摘In this paper,we have studied the total ionizing dose(TID)radiation response up to 2 Mrad(Si)of silicon-oxide-nitride-oxide-silicon(SONOS)memory cells and memory circuits,fabricated in a 130 nm complimentary metal-oxide-semiconductor(CMOS)SONOS technology.We explored the threshold voltage(VT)degradation mechanism and found that the VT shifts of SONOS cells depend on the charge state;simply programming the cell to a higher VT cannot compensate for the radiation induced VT loss.The off-state current(Ioff)increase in the SONOS cell is also studied in this paper.Both VT and Ioffdegradation would affect the memory system.Read data failures are mainly caused by VT shifts under irradiation,and program and erase failures are mainly caused by increased Ioff,which overloads the charge pumping circuit.By varying the reference current,our 4 Mb NOR flash chip has the potential to survive a radiation dose of 1 Mrad(Si)in read mode. | QIAO FengYing PAN LiYang YU Xiao MA HaoZhi WU Dong XU Jun | 2014 | Science China(Information Sciences)2014，57，6： | 3 |
| 10 | Characterization of oil component and solid particle of oily sludge treated by surfactant-assisted ultrasonication显示文摘The ultrasonic technique has been demonstrated to be a promising method for the disposal of hazardous oily sludge.However,the separation of oil from the surfaces of the solid particles is still difficult due to the strong interaction between the oil and solid particle.In this study,three types of surfactants were used to assist the ultrasonic treatment of oily sludge.The oil component,surface composition,and structure of the solid particle were determined.The results showed that different surfactants had different oil removal abilities.In the three surfactant-assisted sonication systems,the oil removal rate increased during the starting reaction period and then decreased with longer sonication time.The results of four components analysis suggested that surfactant easy to be ionized in water posed a better removal effect on resins,while the amphiphilic surfactant preferred saturates,aromatics and asphaltenes.The morphology analysis indicated that particle size was shattered into smaller ones by the ultrasonic process,and the wettability of the solid surface also changed during this treatment.The characterization of the oil component and solid particle during surfactant-assisted ultrasonication treatment will help to better understand the separation of oil from oily sludge and improve the oil recovery efficiency from oily sludge. | Zuhong Lin Fushuai Xu Lili Wang Liyang Hu Lingfu Zhu Jie Tan Zhifeng Li Tingting Zhang | 2021 | Chinese Journal of Chemical Engineering2021，34，6： | 2 |
| 11 | Multiple processes affecting surface seawater N_2O satura-tion anomalies in tropical oceans and Prydz Bay, Antarc-tica显示文摘We analyzed the N2O content of surface seawater sampled from Prydz Bay, Antarctica, on a cruise track between 30°S and 30°N during the twenty-second Chinese National Antarctic Research Expedition during austral summer, 2006. The surface water showed an average pN 2 O value of 311.9±7.6 nL·L -1 (14.1±0.4 nmol·L -1 ), which was slightly undersaturated. The air-sea N 2 O flux in the region was -0.3±0.8 μmol·m -2 ·d -1 ; however, N 2 O in the surface water was oversaturated in most stations along the cruise track. Saturation anomalies were greater than 10%, with a maximum of 54.7% being observed at the Equator, followed by 31% at 10°N in the Sulu Sea. The air-sea fluxes at these locations were 12.4 and 4 μmol·m -2 ·d -1 , respectively. Overall, the results indicated that surface water in Prydz Bay was near equilibrium with atmospheric N 2 O, and that ocean waters in lower latitudes acted as a N 2 O source. Physical processes such as stratification, ice-melt water dilution, and solar radiation dominate the factors leading to N 2 O saturation of surface water of Prydz Bay, while biological production and upwelling are primarily responsible for the N 2 O oversaturation of surface water observed in subtropical and tropical regions along the cruise track. | CHEN Liqi ZHAN Liyang XU Suqing ZHANG Jiexia ZHANG Yuanhui XU Guojie | 2012 | Advances in Polar Science2012，23，2： | 2 |
| 12 | Dehydro- genation of methyl-cyclohexane under multiphase reaction condition显示文摘 | Zhang Liyang Xu Guohua An Yue | 2006 | International Journal of Hydrogen Energy2006，319，15： | 1 |
| 13 | Conglomerates and sandstones from the Yintun Formation in Northern Liaoning Province: Implications for the Huronian Glaciation and reconstruction of the Columbia supercontinent显示文摘New U-Pb zircon geochronology data suggest that the Yintun Formation is younger man ~ 1895 Ma and is late Paleoproterozoic.The Yintun Formation may represent the first sedimentary cover of the North China Craton(NCC)based on its stratigraphic sequence and the ages of its sediments.Gravels in the conglomerates from the lower member of the Yintun Formation are characterized by obvious glacial origins.It is possible that these glacial gravels are the re-deposited sedimentary products of the Huronian Glaciation from the Indian continent.This can also provide sedimentary evidence for the relative location of the Indian continent and the NCC in the reconstruction of the Columbia supercontinent. | Liyang Zhang Bei Xu Zhiwen Luo Wen Liao | 2016 | Science Bulletin2016，61，17： | 1 |
| 14 | Improving Data Recovery in MPEG - 4 显示文摘 | Liyang XU Sunil K Mrinal M | 2005 | IEICE Trans D : Information2005，88，： | 1 |
| 15 | Decreased expression of micro RNA ‐21 correlates with the imbalance of Th17 and Treg cells in patients with rheumatoid arthritis显示文摘 | Liyang Dong Xuefeng Wang Jun Tan Hao Li Wei Qian Jianguo Chen Qiaoyun Chen Jun Wang Wenlin Xu Caihua Tao Shengjun Wang | 2014 | J. Cell. Mol. Med2014，，： | 1 |
| 16 | Responses of potential ammonia oxidation and ammonia oxidizers community to arsenic stress in seven types of soil显示文摘Soil arsenic contamination is of great concern because of its toxicity to human,crops,and soil microorganisms.However,the impacts of arsenic on soil ammonia oxidizers communities remain unclear.Seven types of soil spiked with 0 or 100 mg arsenic per kg soil were incubated for 180 days and sampled at days 1,15,30,90 and 180.The changes in the community composition and abundance of ammonia oxidizing bacteria(AOB)and ammonia oxidizing archaea(AOA)were analyzed by terminal restriction fragment length polymorphism(TRFLP)analysis,clone library sequencing,and quantitative PCR(qPCR)targeting amoA gene.Results revealed considerable variations in the potential ammonia oxidation(PAO)rates in different soils,but soil PAO was not consistently significantly inhibited by arsenic,probably due to the low bioavailable arsenic contents or the existence of functional redundancy between AOB and AOA.The variations in AOB and AOA communities were closely associated with the changes in arsenic fractionations.The amoA gene abundances of AOA increased after arsenic addition,whereas AOB decreased,which corroborated the notion that AOA and AOB might occupy different niches in arsenic-contaminated soils.Phylogenetic analysis of amoA gene-encoded proteins revealed that all AOB clone sequences belonged to the genus Nitrosospira,among which those belonging to Nitrosospira cluster 3a were dominant.The main AOA sequence detected belonged to Thaumarchaeal Group 1.1b,which was considered to have a high ability to adapt to environmental changes.Our results provide new insights into the impacts of arsenic on the soil nitrogen cycling. | Yanan Wang Xibai Zeng Yang Zhang Nan Zhang Liyang Xu Cuixia Wu | 2023 | Journal of Environmental Sciences2023，，5： | 0 |
| 17 | IMPROVEMENT OF TCAM-BASED PACKET CLASSIFICATION ALGORITHM显示文摘The feature of Ternary Content Addressable Memories(TCAMs) makes them particularly attractive for IP address lookup and packet classification applications in a router system. However,the limitations of TCAMs impede their utilization. In this paper,the solutions for decreasing the power consumption and avoiding entry expansion in range matching are addressed. Experimental results demonstrate that the proposed techniques can make some big improvements on the performance of TCAMs in IP address lookup and packet classification. | Xu Zhen Zhang Jun Rui Liyang Sun Jun | 2008 | Journal of Electronics(China)2008，25，2： | 0 |
| 18 | Engineered human spinal cord-like tissues with dorsal and ventral neuronal progenitors for spinal cord injury repair in rats and monkeys显示文摘Transplanting human neural progenitor cells is a promising method of replenishing the lost neurons after spinal cord injury (SCI), but differentiating neural progenitor cells into the diverse types of mature functional spinal cord neurons in vivo is challenging. In this study, engineered human embryonic spinal cord-like tissues with dorsal and ventral neuronal characters (DV-SC) were generated by inducing human neural progenitor cells (hscNPCs) to differentiate into various types of dorsal and ventral neuronal cells on collagen scaffold in vitro. Transplantation of DV-SC into complete SCI models in rats and monkeys showed better therapeutic effects than undifferentiated hscNPCs, including pronounced cell survival and maturation. DV-SC formed a targeted connection with the host’s ascending and descending axons, partially restored interrupted neural circuits, and improved motor evoked potentials and the hindlimb function of animals with SCI. This suggests that the transplantation of pre-differentiated hscNPCs with spinal cord dorsal and ventral neuronal characteristics could be a promising strategy for SCI repair. | Bai Xu Dingyang Liu Weiyuan Liu Ge Long Wenbin Liu Yayu Wu Xinghui He Yeyu Shen Peipei Jiang Man Yin Yongheng Fan He Shen Liyang Shi Qi Zhang Weiwei Xue Chen Jin Zhenni Chen Bing Chen Jiayin Li Yali Hu Xing Li Zhifeng Xiao Yannan Zhao Jianwu Dai | 2023 | Bioactive Materials2023，，9： | 0 |
| 19 | Soft Sensor with Light and Efficient Multi-scale Feature Method for Multiple Sampling Rates in Industrial Processing显示文摘In industrial process control systems,there is overwhelming evidence corroborating the notion that economic or technical limitations result in some key variables that are very difficult to measure online.The data-driven soft sensor is an effective solution because it provides a reliable and stable online estimation of such variables.This paper employs a deep neural network with multiscale feature extraction layers to build soft sensors,which are applied to the benchmarked Tennessee-Eastman process(TEP)and a real wind farm case.The comparison of modelling results demonstrates that the multiscale feature extraction layers have the following advantages over other methods.First,the multiscale feature extraction layers significantly reduce the number of parameters compared to the other deep neural networks.Second,the multiscale feature extraction layers can powerfully extract dataset characteristics.Finally,the multiscale feature extraction layers with fully considered historical measurements can contain richer useful information and improved representation compared to traditional data-driven models. | Dezheng Wang Yinglong Wang Fan Yang Liyang Xu Yinong Zhang Yiran Chen Ning Liao | 2024 | Machine Intelligence Research2024，21，2： | 0 |
| 20 | Optimization of Processing Technology for Roasted Licorice with Water by Orthogonal Method显示文摘[Objectives]To optimize the processing technology for roasted licorice with water.[Methods]Through the orthogonal experimental design,taking the water added,moistening time,frying temperature and frying time as the factors,and the content of glycyrrhizin and glycyrrhizic acid as the evaluation index,the processing technology for roasted licorice with water was optimized.[Results]The best processing technology of licorice was as follows:Pure licorice slices were mixed with water and moistened for 3 h,and then fried at 160℃for 6 min.20 kg of water was added to every 100 kg of licorice.[Conclusions]The best processing technology for roasted licorice with water was established,laying a foundation for the research and application of roasted licorice with water and its preparation. | Yali XU Xiaopeng LI Liyang HA Zhilong SHI Jian GU | 2020 | Medicinal Plant2020，11，3： | 0 |
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