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| 1 | Associations of CYP3A4, NR1I2, CYP2C19 and P2RY12 polymorphisms with clopidogrel resistance in Chinese patients with ischemic stroke显示文摘瞄准:在亚洲人口有 antiplatelet 药 clopidogrel 抵抗(CR ) 的高发生。因为 clopidogrel 是 prodrug,编码涉及它的 biotransformation 的酶的基因的多型性可以是主要有影响的因素。这研究的目标是与 ischemic stroke.Methods 在中国病人与 CR 调查 CYP3A4, NR1I2, CYP2C19 和 P2RY12 基因的多型性的协会:有 ischemic 击的 191 个病人的一个总数被注册。病人与 clopidogrel 被对待至少 5 天。血小板功能被轻传播 aggregometry 测量。SNP NR1I2 (rs13059232 ) , CYP3A4 *1G (rs2242480 ) , CYP2C19 *2 (rs4244285 ) 和 P2RY12 (rs2046934 ) 是 genotyped.Results:在这张人口的 CR 率是 36% 。CYP2C19 *2 变体是为 CR 的一个风险因素(*2/*2+wt/*2 对 wt/wt,或:2.366, 95% CI:1.180-4.741, P=0.014 ) ,而 CYP3A4 *1G 变体在 CR 上有保护的效果(*1/*1 对 *1G/*1G+*1/*1G, 或:2.360, 95% CI:1.247-4.468, P=0.008 ) 。NR1I2 (rs13059232 ) 多型性中等与 CR 被联系(CC 对 TT+TC,或:0.533, 95% CI:0.286-0.991, P=0.046 ) 。在 P2RY12 (rs2046934 ) 的 C 等位基因被预言是为 CR 的一个保护的因素(CC+TC 对 TT,或:0.407, 95% CI:0.191-0.867, P=0.018 ) 。另外,一个协会在高血压和 CR (P=0.022 ) 之间被发现 .Conclusion:有 CYP2C19 *2 等位基因和高血压的个人在反血栓治疗期间在 CR 的高风险。CYP3A4 *1G 等位基因, P2RY12 (rs2046934 ) C 等位基因和 NR1I2 (rs13059232 ) CC 遗传型可以是为 CR 的保护的因素。学习的联系 SNP 可能是有用的与 ischemic 击在中国病人预言 clopidogrel 抵抗。 | Rui LIU Zi-yi ZHOU Yi-bei CHEN Jia-li LI Wei-bang YU Xin-meng CHEN Min ZHAO Yuan-qi ZHAO Ye-feng CAI Jing JIN Min HUANG | 2016 | Acta Pharmacologica Sinica2016，37，7： | 39 |
| 2 | The altered expression of inflammation-related microRNAs with microRNA-155 expression correlates with Th17 differentiation in patients with acute coronary syndrome显示文摘MicroRNAs(miRNAs)are a novel class of small,non-coding RNAs that play a significant role in both inflammatory and cardiovascular diseases.Immune cells,especially T helper(Th)cells,are critical in the development of atherosclerosis and the onset of acute coronary syndrome(ACS).To assess whether inflammation-related miRNAs(such as miR-155,146a,21,125a-5p,125b,31)are involved in the imbalance of Th cell subsets in patients with ACS,we measured the expression of related miRNAs in patients with acute myocardial infarction(AMI),unstable angina(UA),stable angina(SA)and chest pain syndrome(CPS);analyzed the relationship between miRNA expression and the frequency of Th cell subsets;and observed the co-expression of miR-155 and IL-17A in peripheral blood mononuclear cells(PBMCs)of patients with ACS.The results showed that the expression of miR-155 in the PBMCs of patients with ACS was decreased by approximately 60%,while the expression of both miR-21 and miR-146a was increased by approximately twofold.The expression patterns of miRNAs in plasma correlated with those in PBMCs,except for miR-21,which was increased by approximately sixfold in the AMI group and showed no significant difference between the UA group and the CPS group.We also found that the expression of miR-155 inversely correlated with the frequency of Th17 cells(r520.896,P,0.01)and that miR-155 was co-expressed with IL-17A in patients with ACS.In conclusion,our study revealed the expression patterns of inflammation-related miRNAs in patients with ACS and found that miR-155 may be associated with Th17 cell differentiation. | Rui Yao Yulan Ma Youyou Du Mengyang Liao Huanhuan Li Wei Liang Jing Yuan Zhijun Ma Xian Yu Hong Xiao Yuhua Liao | 2011 | Cellular & Molecular Immunology2011，8，6： | 32 |
| 3 | Effect of Bcl-2 and Bax on survival of side population cells from hepatocellular carcinoma cells显示文摘AIM: To understand the role and significance of side population (SP) cells from hepatocellular carcinoma (HCC) in hepatocarcinogenesis, development, relapse and metastasis, we simulated the denutrition conditions that cancer cells experience in clinical therapy, observed the different anti-apoptosis ability of SP cells and non-SP cells under such conditions, and established the possible effects of P53, Bcl-2 and Bax on survival of SP cells. METHODS: We used ? ow cytometry to analyze and sort the SP and non-SP cells in established HCC lines MHCC97 and hHCC. We evaluated cell proliferation by methyl thiazolyl tetrazolium (MTT) assay and investigated the expression of p53, bcl-2 and bax genes during denutrition, by RT-PCR and immuno? uorescence staining. RESULTS: The percentage of SP cells in the two established HCC lines was 0.25% and 0.5%, respectively. SP cells had greater anti-apoptosis and proliferation ability than non-SP cells. Expression of Bcl-2 and Bax in SP and non-SP cells differed during denutrition. The former was up-regulated in SP cells, and the latter was up-regulated in non-SP cells. CONCLUSION: It may be that different upstream molecules acted and led to different expression levels of Bcl-2 and Bax in these two cell lines. There was a direct relationship between up-regulation of Bcl-2 and down-regulation of Bax and higher anti-apoptosis ability in SP cells. It may be that the existence and activity of SP cells are partly responsible for some of the clinical phenomena which are seen in HCC, such as relapse or metastasis. Further research on SP cells may have potential applications in the f ield of anticancer therapy. | Jing Fan Ren Li Rui Zhang Hai-Liang Liu Ning Zhang Fu-Qing Zhang Ke-Feng Dou | 2007 | World Journal of Gastroenterology2007，13，45： | 27 |
| 4 | Changes of Urban Wetland Landscape Pattern and Impacts of Urbanization on Wetland in Wuhan City显示文摘In this study,remote sensing data of Wuhan City,Hubei Province,China in 1996-2001 were selected to ex-tract wetland landscape information.Several landscape indices were used to evaluate the changes of landscape pattern within the five years,including patch number,patch density,patch fractal dimension,landscape diversity,dominance,evenness,and fragmentation indexes.Then,transformation probabilities of wetland landscapes into non-wetland land-scapes were calculated based on Markov Model,and on these grounds the relationship between changes of wetland landscape pattern and urban construction was analyzed.The results showed that fragmentation degree of all wetland types increased,lake area declined,and dominance of natural wetland decreased.The reasons for these results were mainly because of urban construction.According to the features of abundant wetland in Wuhan City,we suggested that protection of wetland landscape should cooperate with urban construction,which means wetland should become im-portant part of urban landscape. | WANG Xuelei NING Longmei YU Jing XIAO Rui LI Tao | 2008 | Chinese Geographical Science2008，18，1： | 27 |
| 5 | Springback equation of small curvature plane bending显示文摘A new analytical method for springback of small curvature plane bending is addressed with unloading rule of classical elastic-plastic theory and principle of strain superposition.We start from strain analysis of plane bending which has initial curvature,and the theoretic derivation is on the widely applicable basic hypotheses.The results are unified to geometry constraint equations and springback equation of plane bending,which can be evolved to straight beam plane bending and pure bending.The expanding and setting round process is one of the situations of plane bending,which is a bend-stretching process of plane curved beam.In the present study,springback equation of plane bending is used to analyze the expanding and setting round process,and the results agree with the experimental data.With a reasonable prediction accuracy,this new analytical method for springback of plane bending can meet the needs of applications in engineering. | ZHAO Jun YIN Jing MA Rui MA LiXia | 2011 | Science China(Technological Sciences)2011，54，9： | 27 |
| 6 | Manganese is critical for antitumor immune responses via cGAS-STING and improves the efficacy of clinical immunotherapy显示文摘CD8^+T cell-mediated cancer clearance is often suppressed by the interaction between inhibitory molecules like PD-1 and PD-L1,an interaction acts like brakes to prevent T cell overreaction under normal conditions but is exploited by tumor cells to escape the immune surveillance.Immune checkpoint inhibitors have revolutionized cancer therapeutics by removing such brakes.Unfortunately,only a minority of cancer patients respond to immunotherapies presumably due to inadequate immunity.Antitumor immunity depends on the activation of the cGAS-STING pathway,as STING-deficient mice fail to stimulate tumor-infiltrating dendritic cells(DCs)to activate CD8^+T cells.STING agonists also enhance natural killer(NK)cells to mediate the clearance of CD8^+T cell-resistant tumors.Therefore STING agonists have been intensively sought after.We previously discovered that manganese(Mn)is indispensable for the host defense against cytosolic dsDNA by activating cGAS-STING.Here we report that Mn is also essential in innate immune sensing of tumors and enhances adaptive immune responses against tumors.Mn-insufficient mice had significantly enhanced tumor growth and metastasis,with greatly reduced tumor-infiltrating CD8^+T cells.Mechanically,Mn^2+promoted DC and macrophage maturation and tumor-specific antigen presentation,augmented CD8^+T cell differentiation,activation and NK cell activation,and increased memory CD8^+T cells.Combining Mn^2+with immune checkpoint inhibition synergistically boosted antitumor efficacies and reduced the anti-PD-1 antibody dosage required in mice.Importantly,a completed phase 1 clinical trial with the combined regimen of Mn^2+and anti-PD-1 antibody showed promising efficacy,exhibiting type I IFN induction,manageable safety and revived responses to immunotherapy in most patients with advanced metastatic solid tumors.We propose that this combination strategy warrants further clinical translation. | Mengze Lv Meixia Chen Rui Zhang Wen Zhang Chenguang Wang Yan Zhang Xiaoming Wei Yukun Guan Jiejie Liu Kaichao Feng Miao Jing Xurui Wang Yun-Cai Liu Qian Mei Weidong Han Zhengfan Jiang | 2020 | Cell Research2020，30，11： | 27 |
| 7 | Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system显示文摘MicroRNAs (miRNAs ) 是内长的 noncoding RNA (~ 22 nt ) 在细胞质在 post-transcriptional 水平调整目标基因表示。在房间原子核的要求功能的 argonaute 家庭蛋白质提示了我们假设那 miRNAs 的 miRNAs 和 miRNA 的存在的最近的发现可以也在房间原子核有规章的功能。在这研究,我们证明老鼠 miR-709 主要位于各种各样的房间类型的原子核并且它的原子本地化模式很快在 apoptotic 刺激之上变化。在房间原子核, miR-709 直接在 pri-miR-15a/16-1 上绑在一个 19-nt miR-709 识别元素并且阻止它处理成 pre-miR-15a/16-1,导致 miR-15a/16-1 成熟的抑制。而且,原子 miR-709 通过 miR-15a/16-1 小径参予房间 apoptosis 的规定。在摘要,现在的学习提供一 miRNA 能由直接在原子核指向他们的主要抄本控制另外的 miRNAs 的生物的续生说的第一条证据。 | Rui Tang Limin Li Dihan Zhu Dongxia Hou Ting Cao Hongwei Gu Jing Zhang Junyuan Chen Chen-Yu Zhang Ke Zen | 2012 | Cell Research2012，22，3： | 23 |
| 8 | Bacterial community in sediment from the Western Pacific 'Warm Pool' and its relationship to environment显示文摘Total DNAs were extracted from different sections of deep sea sediment core sample collected from the Western Pacific 'Warm Pool'. The bacterial 16S ribosomal DNA (rDNA) clone libraries were constructed and analyzed by PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing. The bacterial communities in these samples and their relationship to environment were analyzed consequently. The results indicated that among eight main bacterial groups found in these sediments, members of the γ-Proteobacteria were most abundant in each section of sediment core sample and the genus Colwellia belonging to γ-Proteobacteria was dominant in this area. Members of the α-Proteobacteria were found commonly existing in these samples, while members belonging to β-Proteobacteria were seldom detected. The diversity of bacterial communities from different sections of sediment core sample was δ- and ε-Proteo- bacteria and the bacterial group including genera Cytopahga, Flexibacteria and Bacteroides (CFB group). These bacteria all were inversely proportional to the depth of sediment. Phylogenetic analysis showed that there were 18%-30% and 15%-25% of total bacterial communities related to methane and sulfur metabolism respectively in each section of core sample, implicating that the metabolism of sulfur and methane played an important role in the substance and energy cycles of the Western Pacific 'Warm Pool'. | ZENG Runying ZHAO Jing ZHANG Rui LIN Nianwei | 2005 | Science China Earth Sciences2005，48，2： | 21 |
| 9 | Efficient Targeted Genome Modification in Maize Using CRISPR/Cas9 System显示文摘CRISPR(clustered regularly interspaced short palindromic repeats)/Cas9 system, which is a newly developed technology for targeted genome modification, has been successfully used in a number of species. In this study, we applied this technology to carry out targeted genome modification in maize. A marker gene Zmzb7 was chosen for targeting. The sg RNA-Cas9 construct was transformed into maize protoplasts, and indel(insertion and deletion) mutations could be detected. A mutant seedling with an expected albino phenotype was obtained from screening 120 seedlings generated from 10 callus events. Mutation efficiency in maize heterochromatic regions was also investigated. Twelve sites with different expression levels in maize centromeres or pericentromere regions were selected. The sg RNACas9 constructs were transformed into protoplasts followed by sequencing the transformed protoplast genomic DNA. The results show that the genes in heterochromatic regions could be targeted by the CRISPR/Cas9 system efficiently, no matter whether they are expressed or not. Meanwhile, off-target mutations were not found in the similar sites having no PAM(protospacer adjacent motif) or having more than two mismatches. Together, our results show that the CRISPR/Cas9 system is a robust and efficient tool for genome modification in both euchromatic and heterochromatic regions in maize. | Chao Feng Jing Yuan Rui Wang Yang Liu James A. Birchler Fangpu Han | 2016 | Journal of Genetics and Genomics2016，43，1： | 20 |
| 10 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 11 | Infantile hepatic hemangioendothelioma:A clinicopathologic study in a Chinese population显示文摘AIM:To investigate whether the clinicopathologic features of infantile hemangioendothelioma(IHE) of the liver in a Chinese population are similar to the features observed in other races.METHODS:The clinical data,radiological findings,histopathological changes and outcome of 12 cases of IHE diagnosed by the Department of Pathology,West China Hospital over the last 10 years were analyzed retrospectively.Immunohistochemical studies were carried out using antibodies against CD31,CD34,Factor Ⅷ,cytokeratin 8 and cytokeratin 18.RESULTS:The 12 patients were aged from fetal to 5 years(three males and nine females).The tumor was presented with different clinical manifestations,mainly as an asymptomatic,palpable,upper abdominal mass,except for the two fetuses who were detected antena-tally by ultrasound.In one patient,this presentation was accompanied by an initial severe pneumothorax.No symptoms of congestive heart failure were present and neither congenital abnormalities nor vascular tumors in the skin or other organs were found.Laboratory abnormalities included leukocytosis(40%),anemia(60%),thrombocytosis(60%),hyperbilirubinemia(16.7%),abnormal liver function(50%) and increased α-fetoprotein(80%).Based on radiological findings and gross specimens,the tumor presented as a solitary lesion or a multifocal space-occupying lesion.The tumor size ranged from 5.0 cm × 3.5 cm × 2.0 cm to 13.8 cm × 9.0 cm × 7.7 cm,and the 0.2-1.1 cm nodules were diffusely distributed within the multifocal tumor.Seven cases were surgically resected,three cases underwent biopsy and the two fetuses were aborted.Histologically,nine cases were classified as typeⅠ and three as type Ⅱ,presenting aggressive morphologic features,immature vessels,active mitosis and necrosis.An inflammatory component,predominantly eosinophilic granulocytes,sometimes obscured the nature of the tumor.Ten patients are alive after a follow-up of 1-9 years.Based on immunohistochemistry,the endothelial cells in all cases were positive for CD31,CD34 and polyclonal factor Ⅷ antigen,whereas the scattered hyperplasia bile ducts were positive for cytokeratin 8 and cytokeratin 18.CONCLUSION:The clinical manifestations of IHE are non-specific.There is no significant correlation between histological type and prognosis.The clinicopathologic features of IHE in Chinese patients may provide a clue to further evidence-based studies. | Zhang Zhang,Hui-Jiao Chen,Wen-Juan Yang,Hong Bu,Bing Wei,Xiao-Yu Long,Jing Fu,Rui Zhang,Yun-Bi Ni,Hong-Ying Zhang,Department of Pathology,West China Hospital,Sichuan University,Guoxuexiang 37,Chengdu 610041,Sichuan Province,China | 2010 | World Journal of Gastroenterology2010，16，36： | 16 |
| 12 | Comparison of visual performance between conventional LASIK and wavefront-guided LASIK with iris-registration显示文摘背景:激光原位 keratomileusis (LASIK ) 成为了一个有效、通常执行的过程减少折射错误。为了推进,增加手术后的视觉质量,指导波前的折射外科是在设定的外科的一个研究热点。这研究被进行把指导波前的 LASIK 的视敏度,高顺序的错误,和对比敏感与虹登记和常规 LASIK 作比较。方法:109 个病人的 211 只近视眼睛在这未来的研究被注册并且随机把组划分了成二:组(94 只眼睛) 和常规 LASIK 组织的指导波前的 LASIK (wg LASIK )(117 只眼睛) 。Wavescan 波前象差计被用来与 6.0 公里学生尺寸,和 Optec 6500 visual 分析 Zernike 系数和更高的顺序错误的 root-mean-square (RMS ) 功能仪器被用来在两个组在外科前后在 5 空间频率下面测量对比敏感(CS ) 。结果:未改正的视敏度(UCVA ) 和在 wg LASIK 组的吝啬的球形的等价物(SE ) 比在常规 LASIK 的那些显著地好(UCVA, z = 2.339, P = 0.019;SE, t = 2.838, P = 0.005 ) 在在外科以后的 3 个月。而且, Z ( 3 )的增加(-3), Z ( 3 )( 1 ), Z ( 3 )( 3 ), Z ( 4 )( 0 ), Z ( 5 )(-1), Z ( 5 )( 1 ), Z ( 5 )( 5 )和 Z ( 6 )(在 wg LASIK 组的-6)在常规 LASIK 组是比那统计上小的( P <
0.05 )。在 wg LASIK 组,与更高的顺序错误的外科手术前的 RMS 的更高的数量看(RMSh = 0.30 microm ) 在外科(33.3%) 以后在 3 个月在常规 LASIK 组比那些显示出统计上更低的增加(13.5%)(P = 0.004 ) 。并且第 4 顺序球面象差(4thSA ) 和根平均数的价值在 wg LASIK 第 6 顺序错误(RMS6 ) 摆平组是比在在有更高外科手术前的散光(= 1.0D ) 的眼睛的传统的组的那些显著地低的(4thSA, P = 0.03;RMS6, P = 0.02 ) 。Wg LASIK 比相应地外科手术前的价值组织显示出的更好的 CS 价值有或没有在在外科以后的 3 个月的瞪视的根本空间的频率当常规 LASIK 组织时,显示的减少的 CS 与瞪视每度除了 1.5 和 3 个周期珍视。二个组之间的差别是统计上重要的(P <
0.001 ) 。结论:有虹登记的指导波前的 LASIK 是有效的减少特别球形的更高的顺序错误和昏迷错误,并且与常规 LASIK 相比改进手术后的视敏度和对比敏感。有虹登记的指导波前的 LASIK 的应用程序与更高外科手术前的散光对有更高外科手术前的 RMSh 值和眼睛的眼睛特别地合适。 | ZHANG Jing ZHOU Yue-hua WANG Ning-li LI Rui | 2008 | Chinese Medical Journal2008，，2： | 15 |
| 13 | Effects of Plasma Lipids and Statins on Cognitive Function显示文摘 | Rui Li Tian-Jun Wang Pei-Yuan Lyu Yang Liu Wei-Hong Chen Ming-Yue Fan Jing Xu | 2018 | Chinese Medical Journal2018，，4： | 14 |
| 14 | Single and competitive adsorption affinity of heavy metals toward peanut shell-derived biochar and its mechanisms in aqueous systems显示文摘Converting peanut shells into biochar by pyrolysis was considered an environmentally friendly and efficient method for agricultural solid waste disposal.The properties of peanut shell-derived biochar(PBC)under different temperature and its adsorption capacity of heavy metals were investigated.It was found that PBC400 exhibited the highest cumulative capability for heavy metals elimination in single solute because of its high specific surface area and rich functional groups.Furthermore,the competitive adsorption revealed that PBC had a substantial difference in adsorption affinity from diverse heavy metal ions,sorption capacity decreased as Pb2+>Cu2+>Cd2+>Ni2+>Zn2+,which was lower than in a single solute.The adsorption process using selected biochar was optimized with respect to p H,reaction time,adsorbent dose,and initial concentration of heavy metals.The kinetic data was well fitted with PSO model,and the Langmuir model was adopted for adsorption equilibrium data in both cases of single solutes and mixed solutes for all heavy metals,which indicated that the removal course was primarily explained by monolayer adsorption,and chemical adsorption occupied an important role.Therefore,peanut shells derived biochar could be a potential and green adsorbent for wastewater treatment. | Rui Shan Yueyue Shi Jing Gu YazhuoWang Haoran Yuan | 2020 | Chinese Journal of Chemical Engineering2020，28，5： | 14 |
| 15 | Prolonged manganese exposure induces severe deficits in lifespan,development and reproduction possibly by altering oxidative stress response in Caenorhabditis elegans显示文摘We examined the possible multiple defects induced by acute and prolonged exposure to high levels of manganese(Mn) solution by monitoring the endpoints of lifespan,development,reproduction,and stress response.Our data suggest that acute exposure(6 h) to Mn did not cause severe defects of life span,development,and reproduction,similarly,no significant defect could be found in animals exposed to a low concentration of Mn(2.5 μmol/L) for 48 h.In contrast,prolonged exposure(48 h) to high Mn concentrations(75 and 200 μmol/L) resulted in significant defects of life span,development,and reproduction,as well as the increase of the percentage of population with hsp-16.2::gfp expression indicating the obvious induction of stress responses in exposed animals.Moreover,prolonged exposure(48 h) to high concentrations(75 and 200 μmol/L) of Mn decreased the expression levels of antioxidant genes of sod-1,sod-2,sod-3,and sod-4 compared to control.Therefore,prolonged exposure to high concentrations of Mn will induce the severe defects of life span,development,and reproduction in nematodes possibly by affecting the stress response and expression of antioxidant genes in Caenorhabditis elegans. | XIAO Jing | 2009 | Journal of Environmental Sciences2009，21，6： | 13 |
| 16 | Laser performance upgrade for precise ICF experiment in SG-Ⅲ laser facility显示文摘The SG-Ⅲlaser facility(SG-Ⅲ)is the largest laser driver for inertial confinement fusion(ICF)researches in China,which has 48 beamlines and can deliver 180 kJ ultraviolet laser energy in 3 ns.In order to meet the requirements of precise physics experiments,some new functionalities need to be added to SG-Ⅲand some intrinsic laser performances need upgrade.So at the end of SG-Ⅲ's engineering construction,the 2-year laser performance upgrade project started.This paper will introduce the newly added functionalities and the latest laser performance of SG-Ⅲ.With these function extensions and performance upgrade,SG-Ⅲis now fully prepared for precise ICF experiments and solidly paves the way towards fusion ignition. | Wanguo Zheng Xiaofeng Wei Qihua Zhu Feng Jing Dongxia Hu Xiaodong Yuan Wanjun Dai Wei Zhou Fang Wang Dangpeng Xu Xudong Xie Bin Feng Zhitao Peng Liangfu Guo Yuanbin Chen Xiongjun Zhang Lanqin Liu Donghui Lin Zhao Dang Yong Xiang Rui Zhang Fang Wang Huaiting Jia Xuewei Deng | 2017 | Matter and Radiation at Extremes2017，2，5： | 13 |
| 17 | Glycyrrhizic acid attenuates CCl_4-induced hepatocyte apoptosis in rats via a p53-mediated pathway显示文摘AIM: To investigate the effect of glycyrrhizic acid (GA) on carbon tetrachloride (CCl4)-induced hepatocyte apo-ptosis in rats via a p53-dependent mitochondrial path-way. METHODS: Forty-five male Sprague-Dawley rats were randomly and equally divided into three groups, the control group, the CCl4 group, and the GA treatment group. To induce liver fibrosis in this model, rats were given a subcutaneous injection of a 40% solution of CCl4 in olive oil at a dose of 0.3 mL/100 g body weight biweekly for 8 wk, while controls received the same isovolumetric dose of olive oil by hypodermic injection, with an initial double-dose injection. In the GA group,rats were also treated with a 40% solution of CCl4 plus 0.2% GA solution in double distilled water by the intraperitoneal injection of 3 mL per rat three times a week from the first week following previously published methods, with modifications. Controls were given the same isovolumetric dose of double distilled water. Liver function parameters, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were de-termined. Pathologic changes in the liver were detected by hematoxylin and eosin staining. Collagen fibers were evaluated by Sirius red staining. Hepatocyte apoptosis was investigated using the terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) assay and the cleaved caspase-3 immunohistochemistry assay. The expression levels of p53 and apoptosis-related proteins were evaluated by immunohistochemistry or Western blotting analysis. RESULTS: After 8 wk of treatment, GA significantly re-duced serum activity of ALT (from 526.7 ± 57.2 to 342 ± 44.8, P<0.05) and AST (from 640 ± 33.7 to 462.8 ± 30.6, P<0.05), attenuated the changes in liver his-topathology and reduced the staging score (from 3.53 ± 0.74 to 3.00 ± 0.76, P<0.05) in CCl4 -treated rats. GA markedly reduced the positive area of Sirius red and the ratio of the hepatic fibrotic region (from 7.87% ± 0.66% to 3.68% ± 0.32%, P<0.05) compared with the CCl4 group. GA also decreased the expression level of cleaved caspase-3 compared to the CCl4 group. TU-NEL assay indicated that GA significantly diminished the number of TUNEL-positive cells compared with the CCl4 group (P<0.05). GA treatment clearly decreased the level of p53 (P<0.05) detected by immunohis-tochemistry and Western blotting analysis. Compared with the CCl4 group, we also found that GA reduced the Bax/Bcl-2 ratio (P<0.05), the expression of cleaved caspase-3 (P<0.05), cleaved caspase-9 (P<0.05), and inhibited cytochrome C and second mitochondria-derived activator of caspases (Smac) release from mito-chondria to cytoplasm, i.e. , GA reduced the expressionlevel of Smac, which inhibited c-IAP1 activity (P<0.05), ultimately inhibiting the activity of caspase-3, according to Western blotting analysis. As a result, GA suppressed activation of the caspase cascades and prevented he-patocyte apoptosis. | Xiao-Ling Guo Bo Liang Xue-Wei Wang Fu-Gang Fan Jing Jin Rui Lan Jing-Hui Yang Xiao-Chun Wang Lei Jin Qin Cao | 2013 | World Journal of Gastroenterology2013，19，24： | 12 |
| 18 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 19 | Therapeutic effect in patients with coronary heart disease based on information analysis from Traditional Chinese Medicine four diagnostic methods显示文摘OBJECTIVE:To study information gained by Traditional Chinese Medicine(TCM)four diagnostic methods and characteristics of syndrome development before and after treatment in patients with coronary heart disease,and to probe into assessment indexes of therapeutic effects with distinctive TCM features.METHODS:Information from the four diagnostic methods before and after treatment in 100 patients with coronary heart disease was collected using the TCM interrogation scale,a pulse condition instrument,a tongue-face diagnosis instrument,anda voice diagnosis information collection system.Changes in the four diagnostic method results from before and after treatment were analyzed with frequency analysis and t-test methods.RESULTS:Before treatment,deficiency syndrome complicated with hyperactivity of pathogenic factors was most common.After treatment,deficiency syndrome was most common.This change from complex syndromes to single syndromes indicates disease and syndrome alleviation.Frequencies of symptoms gained by interrogation after treatment for syndrome of deficiency of heart-Qi,syndrome of deficiency of heart-Yin,and turbid phlegm syndrome were all less severe than those before treatment.Parameters of face color,color on all sub-regions of tongue,color of tongue fur,septic and greasy fur,and lip color after treatment all had significant changes.After treatment,part of the voice diagnosis parameters in the deficiency of heart-Yin and turbid phlegm syndromes had significant changes,but no significant changes were found in the deficiency of heart-Qi syndrome.CONCLUSION:Use of the TCM four diagnostic methods can provide an effective basis forTCM syndrome diagnosis,observation of development of state of illness,and evaluation of clinical therapeutic effects. | Yiqin Wang Jin Xu Rui Guo Chaoxia Xu Yiming Hao Chunfeng Chen Yijian Hong Xiangqun Xiao Wenjie Xu Jing Hong Zhao Lei | 2014 | Journal of Traditional Chinese Medicine2014，34，1： | 12 |
| 20 | SbHKT1;4, a member of the high‐affinity potassium transporter gene family from Sorghum bicolor, functions to maintain optimal Na^+/K^+ balance under Na^+ stress显示文摘In halophytic plants, the high‐affinity potassium transporter HKT gene family can selectively uptake Kt in the presence of toxic concentrations of Nat. This has so far not been well examined in glycophytic crops. Here, we report the characterization of SbHKT1;4, a member of the HKT gene family from Sorghum bicolor. Upon Nat stress, SbHKT1;4 expression was more strongly upregulated in salt‐tolerant sorghum accession, correlating with a better balanced Nat/ Kt ratio and enhanced plant growth. Heterogeneous expression analyses in mutants of Saccharomyces cerevisiae and Arabidopsis thaliana indicated that overexpressing SbHKT1;4 resulted in hypersensitivity to Nat stress, and such hypersensitivity could be alleviated with the supply of elevated levels of Kt, implicating that SbHKT1;4 may mediate Kt uptake in the presence of excessive Nat. Further electrophysiological evidence demonstrated that SbHKT1;4 could transport Nat and Kt when expressed in Xenopus laevis oocytes. The relevance of the finding that SbHKT1;4 functions to maintain optimal Nat/Kt balance under Nat stress to the breeding of salt‐tolerant glycophytic crops is discussed. | Tian‐Tian Wang Zhi‐Jie Ren Zhi‐Quan Liu Xue Feng Rui‐Qi Guo Bao‐Guo Li Le‐Gong Li Hai‐Chun Jing | 2014 | Journal of Integrative Plant Biology2014，56，3： | 11 |
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