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| 1 | Human umbilical cord-derived mesenchymal stem cell therapy in patients with COVID-19:a phase 1 clinical trial显示文摘No effective drug treatments are available for coronavirus disease 2019(COVID-19).Host-directed therapies targeting the underlying aberrant immune responses leading to pulmonary tissue damage,death,or long-term functional disability in survivors require clinical evaluation.We performed a parallel assigned controlled,non-randomized,phase 1 clinical trial to evaluate the safety of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)infusions in the treatment of patients with moderate and severe COVID-19 pulmonary disease.The study enrolled 18 hospitalized patients with COVID-19(n=9 for each group).The treatment group received three cycles of intravenous infusion of UC-MSCs(3×107 cells per infusion)on days 0,3,and 6.Both groups received standard COVID-treatment regimens.Adverse events,duration of clinical symptoms,laboratory parameters,length of hospitalization,serial chest computed tomography(CT)images,the PaO2/FiO2 ratio,dynamics of cytokines,and IgG and IgM anti-SARS-CoV-2 antibodies were analyzed.No serious UC-MSCs infusion-associated adverse events were observed.Two patients receiving UC-MSCs developed transient facial flushing and fever,and one patient developed transient hypoxia at 12 h post UC-MSCs transfusion.Mechanical ventilation was required in one patient in the treatment group compared with four in the control group.All patients recovered and were discharged.Our data show that intravenous UC-MSCs infusion in patients with moderate and severe COVID-19 is safe and well tolerated.Phase 2/3 randomized,controlled,double-blinded trials with long-term follow-up are needed to evaluate the therapeutic use of UC-MSCs to reduce deaths and improve long-term treatment outcomes in patients with serious COVID-19. | Fanping Meng Ruonan Xu Siyu Wang Zhe Xu Chao Zhang Yuanyuan Li Tao Yang Lei Shi Junliang Fu Tianjun Jiang Lei Huang Peng Zhao Xin Yuan Xing Fan Ji-Yuan Zhang Jinwen Song Dawei Zhang Yanmei Jiao Limin Liu Chunbao Zhou Markus Maeurer Alimuddin Zumla Ming Shi Fu-Sheng Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 16 |
| 2 | Transcriptional activation and phosphorylation of OsCNGC9 confer enhanced chilling tolerance in rice显示文摘Low temperature is a major environmental factor that limits plant growth and productivity.Although transient elevation of cytoplasmic calcium has long been recognized as a critical signal for plant cold tolerance,the calcium channels responsible for this process have remained largely elusive.Here we report that OsCNGC9,a cyclic nucleotide-gated channel,positively regulates chilling tolerance by mediating cytoplasmic calcium elevation in rice(Oryza sativa).We showed that the loss-of-function mutant of OsCNGC9 is defective in cold-induced calcium influx and more sensitive to prolonged cold treatment,whereas OsCNGC9 overexpression confers enhanced cold tolerance.Mechanistically,we demonstrated that in response to chilling stress,OsSAPK8,a homolog of Arabidopsis thaliana OST1,phosphorylates and activates OsCNGC9 to trigger Ca2+influx.Moreover,we found that the transcription of OsCNGC9 is activated by a rice dehydration-responsive element-binding transcription factor,OsDREB1A.Taken together,our results suggest that OsCNGC9 enhances chilling tolerance in rice through regulating cold-induced calcium influx and cytoplasmic calcium elevation. | Jiachang Wang Yulong Ren Xi Liu Sheng Luo Xiao Zhang Xin Liu Qibing Lin Shanshan Zhu Hua Wan Yang Yang Yu Zhang Bin Lei Chunlei Zhou Tian Pan Yongfei Wang Mingming Wu Ruonan jing Yang Xu Meng Han Fuqing Wu Cailin Lei Xiuping Guo Zhijun Cheng Xiaoming Zheng Yihua Wang Zhigang Zhao Ling Jiang Xin Zhang Yong-Fei Wang Haiyang Wang Jianmin Wan | 2021 | Molecular Plant2021，14，2： | 10 |
| 3 | Electro-conductive Nanocrystalline Cellulose Film Filled with TiO_2-ReducedGraphene Oxide Nanocomposite显示文摘Imparting electro-conductive properties to nanocellulosebased products may render them suitable for applications in electronics,optoelectronics, and energy storage devices. In the present work, an electroconductive nanocrystalline cellulose(NCC) film filled with TiO2-reducedgraphene oxide(TiO2-RGO) was developed. Initially, graphene oxide(GO) was prepared using the modified Hummers method and thereafter photocatalytically reduced using TiO2 as a catalyst. Subsequently, an electro-conductive NCC film was prepared via vacuum filtration with the as-prepared TiO2-RGO nanocomposite as a functional filler. The TiO2-RGO nanocomposite and the NCC/TiO2-RGO film were systematically characterized. The results showed that the obtained TiO2-RGO nanocomposite exhibited reduced oxygen-containing group content and enhanced electroconductivity as compared with those of GO. Moreover, the NCC film filled with TiO2-RGO nanocomposite displayed an electro-conductivity of up to 9.3 S/m and improved mechanical properties compared with that of the control. This work could provide a route for producing electro-conductive NCC films, which may hold significant potential as transparent flexible substrates for future electronic device applications. | RuoNan Zhao YanJun Tang XiaoChuang Shen XingHua Hong YiMing Zhou | 2018 | Paper And Biomaterials2018，3，4： | 5 |
| 4 | Synthesis and characterization of a novel binuclear iron phthalocyanine/reduced graphene oxide nanocomposite for non-precious electrocatalyst for oxygen reduction显示文摘Binuclear iron phthalocyanine/reduced graphene oxide(bi-Fe Pc/RGO) nanocomposite with good electrocatalytic activity for ORR in alkaline medium was prepared in one step. High angle annular dark field image scanning transmission electron microscopy(HAADF-STEM) and energy dispersive X-ray spectroscopy element mapping results show bi-Fe Pc was uniformly distributed on RGO. An obvious cathodic peak located at about-0.23 V(vs. SCE) in CV and an onset potential of-0.004 V(vs. SCE) in LSV indicate the as-prepared bi-Fe Pc/RGO nanocomposite possesses high activity which is closed to Pt/C for ORR. The ORR on bi-Fe Pc/RGO nanocomposite follows four-electron transfer pathway in alkaline medium. Compared with Pt/C, there is only a slight decrease(about 0.02 V vs. SCE) for bi-Fe Pc/RGO nanocomposite when the methanol exists. The excellent activity and methanol tolerance in alkaline solutions proves that bi-Fe Pc/RGO nanocomposite could be considered as a promising cathode catalyst for alkaline fuel cells. | Ruonan Li Dongtang Zhang Yingyan Zhou Xiayan Wang Guangsheng Guo | 2016 | Science China Chemistry2016，59，6： | 3 |
| 5 | Exposure to dibutyl phthalate impairs lipid metabolism and causes inflammation via disturbing microbiota-related gut-liver axis显示文摘Dibutyl phthalate(DBP),a kind of typical environm ental pollutant,is widely used as plasticizers,and its neurotoxicity and developm ental toxicity have been found in recent years.However,whether oral DBP exposure w ill affect the homeostasis of gut m icrobiota and its adverse response in liver of m am m alians remain unclear.In the present study,10-week experimental cycles of vehicle or DBP(0.1 and 1mg/kg)were given to 6-week-old C57BL/6J mice by oral gavage.Our results revealed that the body weight of mice was increased after exposure to both low and high doses of DBP.The serum levels of hepatic triglyceride and total cholesterol were significantly increased in response to both doses of DBP.In addition,some pivotal genes related to lipogenesis were also increased in liver at the mRNA level.Evaluation of gut m icrobiota by 16S rRNA sequencing technology showed that 0.1 mg/kg DBP exposure significantly affected gut microbiota at the phylum and genus levels.Moreover,DBP exposure decreased mucus secretion and caused inflam m ation in the gut,leading to the im pairm ent of intestinal barrier function.Exposure to OBP inhibited the expression of peroxisome proliferator-activated receptor-y and activated the expression of nuclear factor kappa B.In addition,DBP exposure increased the level of lipopolysaccharide in serum,and increased the expression of toll-like receptor 4 and the levels of inflam m atory cytokines,such as interleukin(IL)-1β,IL-6,and tumor necrosis factor alpha,in the liver.These results indicated that exposure to DBP disturbed the homeostasis of gut m icrobiota,induced hepatic lipid m etabolism disorder,and caused liver inflam mation in mice via the related gut-liver axis signaling pathways. | Ze Xiong Yuyao Zeng Jiafeng Zhou Ruonan Shu Xiaoxian Xie Zhengwei Fu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，12： | 2 |
| 6 | Bio-ecological remediation of freshwater aquaculture environments:A systematic review and bibliometric analysis显示文摘Freshwater aquaculture plays an important role in the global aquaculture industry.The success of freshwater aquaculture relies on the integration of bio-ecological remediation technologies to ensure its sustainable development.This paper systematically reviews the global literatures on bio-ecological remediation of freshwater aquaculture environments from 1990 to 2021,and discusses in detail the research progress and status quo in this field using both bibliometric analysis and content analysis.Our results indicate that the number of publications per year has increased year by year,with a particularly rapid increase after 2015.Institutions and researchers from China and the USA publish the greatest number of papers and contribute the most to this field.By analyzing the research progress in this field,we also find that the protection and restoration of freshwater aquaculture environments has received attention since as early as 1990,such as the application of constructed wetlands and recirculating aquaculture systems.Recently,researchers have increasingly focused on the use of microbial communities in remediation.Furthermore,vanRijn,J's papers are of great value to the research in this field.In content analysis,the application of primary technologies,including plant selection,aquatic animals,microorganisms,and biological filtration and purification equipment,as well as ecological engineering technologies with combinations of various types of organisms,is described to improve the effectiveness and efficiency of remediation.The performance,advantages,disadvantages,or limitations and prospects of these technology applications are analyzed in detail.By comparison,ecological engineering is shown to be the best remediation technology,being most comprehensive,and well-established.Taken together,this paper composes and summarizes existing work in this field,and provides guidance for future research on the improvement of existing technologies and the development of new technologies,with the aim of enhancing remediation capability,improving ecological sustainability and ensuring economic viability. | Qiaohong Zhou Kaixuan Wu Lu Yao Ruonan Chen Shouzhuang Liu Hao Xing Linmei Nie Zhenbin Wu | 2024 | Water Biology and Security2024，3，1： | 0 |
| 7 | Design of the rare-earth-containing materials based on the micro-alloying phase equilibria, phase diagrams and phase transformations显示文摘Rare-earth(RE)elements,known as“industrial vitamins”,have permeated modern lives,especially in high-tech applications.Although the RE elements possess close chemical similarities and have been treated as“one element”in the periodic table,their characteristics differ from each other.The RE microalloying effect is the crux to ameliorate the physicomechanical and thermochemical properties of materials,thereby the study of RE-related phase diagrams becomes indispensable to the design and optimization of RE-containing materials.However,in reality,the knowledge base in this area is considerably scarce compared with that of other commonly-used elements.In this work,the phase equilibria,phase diagrams,phase transformations,and some recent examples of RE-containing materials design are summarized,with which one can predict the RE solubilities,the RE precipitates,as well as the corresponding service behaviors.The attainment of enhanced materials’properties suggests that the thermodynamic rules extracted from the phase diagrams could serve as fundamental criteria for the successful development of novel RE-containing materials. | Kai Xu Chengcheng Zhan Ming Lou Xuelian Xiao Ruonan Zhou Fangming Wang Xiaofei Hu Yuan Yuan Keke Chang | 2023 | Journal of Materials Science & Technology2023，，20： | 0 |
| 8 | Diagnosis and Treatment Guidelines for Mesenchymal Stem Cell Therapy for Coronavirus Disease 2019(Beijing,2021)显示文摘The coronavirus disease 2019(COVID-19)can be caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,and has led to millions of deaths among more than 100 million infected people around the world according to the declaration from World Health Organization.Dysregulated immune response of both the innate and adaptive immune systems is subsistent on COVID-19 patients,of which the degree are associated with disease severity,lung damage and long term functional disability.Current treatment options have included antiretroviral drugs,anti-inflammatory factors,antibodies,immune checkpoint inhibitors,and convalescent plasma therapy.More recently,mesenchymal stem cell(MSC)therapy has been explored for the management and control of COVID-19,particularly with the aim of preventing or at least mitigating respiratory co-morbidities.Though the safety and efficacy of stem cell therapy have been validated in multiple phase I–III clinical trials,to date,no standardized stem cell preparation,administration dosage or interval,product QA/QC testing,storage,transportation,or disposal protocols have been established.The present paper proposes a systematic methodology that addresses all the foregoing process steps and evaluation criteria for the efficacious and safe administration of MSCs in the treatment of patients infected with COVID-19. | Ruonan Xu Lei Shi Wei-Fen Xie Zhe Xu Fan-Ping Meng Jun-Liang Fu Xin Yuan Lei Huang Ming Shi Yonggang Li Yuanyuan Li Chen Yao Yu Zhang Zhongmin Liu Chenyan Gao Zhaohui Wu Shufang Meng Weidong Han Charlie Xiang Gui-Qiang Wang Taisheng Li Xiaoying Wang Yunxia Sun Zunyou Wu Wenhong Zhang Chunhua Zhao Yu Hu Tao Cheng Yuquan Wei Qi Zhou Fu-Sheng Wang | 2021 | Infectious Diseases & Immunity2021，1，2： | 0 |
| 9 | Clinical Epidemiology,Illness Profiles,and the Implication of COVID-19 Before and After the Nationwide Omicron Outbreak During the Winter of 2022显示文摘China.In this study,we aimed to investigate the clinical characteristics and outcomes of patients with coronavirus disease 2019(COVID-19)in the Beijing region.Methods:In this retrospective study,we enrolled inpatients admitted for COVID-19 in the Fifth Medical Center of Chinese PLA General Hospital in Beijing between November 10,2022,and January 30,2023.Demographic and clinical features and treatment outcomeswere comprehensively analyzed.We used logistic regression and linear regression analyses to explore the risk factors associated with disease severity and time of nucleic acid conversion,respectively.Results:A total of 1010 hospitalized patients with COVID-19 were enrolled.The median age was 43.0 years(interquartile range,28.0–63.0),and patients aged<60 years and≥60 years comprised 71.2%and 28.8%of total included patients,respectively.The clinical classification of mild(74.6%,753/1010),moderate(21.0%,212/1010),severe(2.7%,27/1010),and unidentified(1.8%,18/1010)was separately recorded;1005 patients were discharged,and 5 patients died in the hospital.The outbreak of the emerging epidemic witnessed an evident increase in the proportion of moderate(42.9%vs.16.4%)and severe(10.3%vs.1.1%)cases after December 7,2022.Patients with a moderate/severe classification had higher levels of procalcitonin,IL-6,serum ferritin,C-reactive protein,lactic dehydrogenase,serum urea nitrogen,and D-dimer and lower counts of CD4+T,CD8+T,and B cells(all P<0.001).Multivariable regression analysis revealed that increased odds of disease severity were associated with the following factors:age≥60 years,IL-6>7 pg/mL,lactic dehydrogenase level>245 U/L,cough,and fever at admission.Age≥80 years and chronic lung diseasewere independent risk factors in the nonmild group in elderly patients.In addition,the duration for nucleic acid to turn negativewas approximately 5.0 d(interquartile range,3.0–7.0).Prolonged time of nucleic acid conversion was associated with age≥60 years,serum urea nitrogen level>8.2 mmol/L,neutrophil count>7×10^(9)/L,and the presence of a chronic lung disease or carcinoma.Finally,unvaccinated patients accounted for 37.3%of enrolled patients;children and the elder people accounted for approximately half of that.The univariable analysis found that booster doses reduced disease severity and shortened the time of nucleic acid conversion in elderly patients.Conclusions:The outbreak ofOmicron rapidly increased the number of patientswith COVID-19 in Beijing.In elderly patients,booster doses may reduce disease severity and shorten the time of nucleic acid conversion.Healthcare systems should be optimized before an emerging epidemic outbreak. | Yuming Guo Zhe Xu Wen-Xin Wang Cheng Zhen Jinhua Hu Jinsong Mu Chengcheng Ji Xin Yuan Ruonan Xu Lei Huang Lei Shi Fanping Meng Junliang Fu Shuangnan Zhou Siyu Wang Fengyi Li Bo Tu Dawei Zhang Huihuang Huang Yufeng Mao Wen Xu Chao Zhang Xiuying Mu Jun Zhao Bo Jin Haibin Su Yinying Lu Yongqian Cheng Dong Ji Shaoli You Jinghui Dong Changchun Liu Mengmeng Zhang Yuanyuan Li Tianjun Jiang Yonggang Li Fu-Sheng Wang | 2023 | Infectious Diseases & Immunity2023，3，4： | 0 |
| 10 | HLA-mismatched allogeneic adoptive immune therapy in severely immunosuppressed AIDS patients显示文摘Severely immunosuppressed AIDS patients with recurrent opportunistic infections(Ols)represent an unmet medical need even in the era of antiretroviral therapy(ART).Here we report the development of a human leukocyte antigen(HLA)-mismatched allogeneic adaptive immune therapy(AAIT)for severely immunosuppressed AIDS patients.Twelve severely immunosuppressed AIDS patients with severe Ols were enrolled in this single-arm study.Qualified donors received subcutaneous recombinant granulocyte-colony-stimulating factor twice daily for 4-5 days to stimulate hematopoiesis.Peripheral blood mononuclear cells were collected from these donors via leukapheresis and transfused into the coupled patients.Clinical,immunological,and virological parameters were monitored during a 12-month follow-up period.We found AAIT combined with ART was safe and well-tolerated at the examined doses and transfusion regimen in all 12 patients.Improvements in clinical symptoms were evident throughout the study period.All patients exhibited a steady increase of peripheral CD4^(+)T cells from a median 10.5 to 207.5 cells/μl.Rapid increase in peripheral CD8^(+)T-cell count from a median 416.5 to 1206.5 cells/μl was found in the first 90 days since initiation of AAIT.In addition,their inflammatory cytokine levels and HIV RNA viral load decreased.A short-term microchimerism with donor cells was found.There were no adverse events associated with graft-versus-host disease throughout the study period.Overall,AAIT treatment was safe,and might help severely immunosuppressed AIDS patients to achieve a better immune restoration.A further clinical trial with control is necessary to confirm the efficacy of AAIT medication. | Ruonan Xu Ji-Yuan Zhang Bo Tu Zhe Xu Hui-Huang Huang Lei Huang Yan-Mei Jiao Tao Yang Chao Zhang En-Qiang Qin Tian-Jun Jiang Yun-Bo Xie Yuan-Yuan Li Lei Jin Chun-Bao Zhou Ming Shi Mei Guo Hui-Sheng Ai Linqi Zhang Fu-Sheng Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 0 |
| 11 | Outage-driven link selection for secure buffer-aided networks显示文摘In this paper, we investigate secure communication in a two-hop wireless network, where multiple buffer-aided relays assist to securely forward data from the sender to the destination threatened by a passive eavesdropper. To satisfy the security and delay requirements, we propose a novel link selection policy without the instantaneous wiretap channel state information. Different from the most current link selection policies,the proposed link selection policy is designed by joint considering the link secrecy outage probability and the buffer states. To evaluate the secrecy performance, we first characterize the system state transition matrix and the stationary state probability, and then derive the closed-form expressions for the secrecy outage probability and the secrecy rate. Moreover, we formulate a framework based on the queuing theory to analyze the end-to-end information delay at the source, the relays, and the queues, and derive the closed-form expression for the information delay. Finally, we conduct simulations to validate our theoretical performance analysis, and verify the performance improvement of the proposed outage-driven secure transmission scheme in terms of secrecy outage probability and information delay. | Dawei WANG Tianmi HE Fuhui ZHOU Julian CHENG Ruonan ZHANG Qihui WU | 2022 | Science China(Information Sciences)2022，65，8： | 0 |
| 12 | Young Leaf White Stripe encodes a P-type PPR protein required for chloroplast development显示文摘Pentatricopeptide repeat(PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice(Oryza sativa), the detailed molecular functions of many PPR proteins remain unclear.Here, we characterized a rice young leaf white stripe(ylws) mutant, which has defective chloroplast development during early seedling growth.Map-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear-and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2,and rps12, and editing of ndhA, ndhB, and rps14transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development. | Jie Lan Qibing Lin Chunlei Zhou Xi Liu Rong Miao Tengfei Ma Yaping Chen Changling Mou Ruonan Jing Miao Feng Thanhliem Nguyen Yulong Ren Zhijun Cheng Xin Zhang Shijia Liu Ling Jiang Jianmin Wan | 2023 | Journal of Integrative Plant Biology2023，65，7： | 0 |
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