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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility显示文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF. | Lijun Ding Guijun Yan Bin Wang Lu Xu Yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang Yanjun Yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou Yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun | 2018 | Science China(Life Sciences)2018，61，12： | 43 |
| 3 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 4 | Heterogeneous oxidation of cyclohexanone catalyzed by TS-1:Combined experimental and DFT studies显示文摘 | Changjiu Xia Long Ju Yi Zhao Hongyi Xu Bin Zhu Feifei Gao Min Lin Zhenyu Dai Xiaodong Zou Xingtian Shu | 2015 | 催化学报2015，36，6： | 11 |
| 5 | Review of Remotely Sensed Imagery Classification Patterns Based on Object-oriented Image Analysis显示文摘With the wide use of high-resolution remotely sensed imagery, the object-oriented remotely sensed informa- tion classification pattern has been intensively studied. Starting with the definition of object-oriented remotely sensed information classification pattern and a literature review of related research progress, this paper sums up 4 developing phases of object-oriented classification pattern during the past 20 years. Then, we discuss the three aspects of method- ology in detail, namely remotely sensed imagery segmentation, feature analysis and feature selection, and classification rule generation, through comparing them with remotely sensed information classification method based on per-pixel. At last, this paper presents several points that need to be paid attention to in the future studies on object-oriented RS in- formation classification pattern: 1) developing robust and highly effective image segmentation algorithm for multi-spectral RS imagery; 2) improving the feature-set including edge, spatial-adjacent and temporal characteristics; 3) discussing the classification rule generation classifier based on the decision tree; 4) presenting evaluation methods for classification result by object-oriented classification pattern. | LIU Yongxue LI Manchun MAO Liang XU Feifei HUANG Shuo | 2006 | Chinese Geographical Science2006，16，3： | 9 |
| 6 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 7 | Xenograft tumors derived from malignant pleural effusion of the patients with non-small-cell lung cancer as models to explore drug resistance显示文摘Background:Non-small cell lung cancer(NSCLC)patients with epidermal growth factor receptor(EGFR)mutations or anaplastic lymphoma kinase(ALK)fusions show dramatic responses to specific tyrosine kinase inhibitors(TKIs);however,after 10-12 months,secondary mutations arise that confer resistance.We generated a murine xenograft model using patient-derived NSCLC cells isolated from the pleural fluid of two patients with NSCLC to investigate the mechanisms of resistance against the ALK-and EGFR-targeted TKIs crizotinib and osimertinib,respectively.Methods:Genotypes of patient biopsies and xenograft tumors were determined by whole exome sequencing(WES),and patients and xenograft-bearing mice received targeted treatment(crizotinib or osimertinib)accordingly.Xenograft mice were also treated for prolonged periods to identify whether the development of drug resistance and/or treatment responses were associated with tumor size.Finally,the pathology of patients biopsies and xenograft tumors were compared histologically.Results:The histological characteristics and chemotherapy responses of xenograft tumors were similar to the actual patients.WES showed that the genotypes of the xenograft and patient tumors were similar(an echinoderm microtu-bule-associated protein-like 4-ALK(EML4-ALK)gene fusion(patient/xenograft:CTC15035EML4-ALK)and EGFR L858R and T790M mutations(patient/xenograft:CTC15063EGFR L858R,T790M)).After continuous crizotinib or osimertinib treatment,WES data suggested that acquired ALK E1210K mutation conferred crizotinib resistance in the CTC15035EML4-ALK xenograft,while decreased frequencies of EGFR L858R and T790M mutations plus the appearance of v-RAF murine sarcoma viral oncogene homolog B(BRAF)G7V mutations and phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha(PIK3C2A)A86fs frame shift mutations led to osimertinib resistance in the CTC15063EGFR L858R,T790M xenografts.Conclusions:We successfully developed a new method of generating drug resistance xenograft models from liquid biopsies using microfluidic technology,which might be a useful tool to investigate the mechanisms of drug resist-ance in NSCLC. | Yunhua Xu Feifei Zhang Xiaoqing Pan Guan Wang Lei Zhu Jie Zhang Danyi Wen Shun Lu | 2018 | Cancer Communications2018，38，1： | 7 |
| 8 | A lentiviral vector-based therapeutic vaccine encoding Ag85B-Rv3425 potently increases resistance to acute tuberculosis infection in mice显示文摘为 multidrug 抵抗的肺结核(TB ) 和到为 TB 的新奇治疗学的途径的发展的广泛地药抵抗的 TB 电话注意的很少处理选择。因为他们能导致抗原特定的细胞的有免疫力的回答,治疗学的疫苗正在答应候选人,它在 Mycobacterium 肺结核(鱼雷快艇) 的消除起一个重要作用。在这研究,新奇 lentiviral 向量为交付鱼雷快艇特定的熔化蛋白质 Ag85B-Rv3425 的治疗学的疫苗被构造。结果证明这支 recombinant lentivirus 疫苗单个注射的能在老鼠触发抗原特定的 Th1 类型有免疫力的回答。更重要地,有急性感染的老鼠在肺和脾由显著地减少的鱼雷快艇负担从这支疫苗的一个单个剂量的政府有益于很多以及与未经治疗的老鼠相比在肺稀释了损害。这些结果为 TB 的免疫疗法显示了这支新奇疫苗的好前景。 | Enzhuo Yang Feifei Wang Ying Xu Honghai Wang Yong Hu Hongbo Shen Zheng W. Chen | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 6 |
| 9 | Synthesis of magnetic polyphosphazene-Ag composite particles as surface enhanced Raman spectroscopy substrates for the detection of melamine显示文摘Magnetic polyphosphazene(MPZS) particles coated by Ag nanoparticles(MPZS-Ag) have been developed as surface enhanced Raman spectroscopy(SERS) substrates for sensitive detection of melamine in aqueous solutions and milk samples.5,5’-Dithiobis-(2-nitrobenzoic acid)(DTNB) was used as model analyte to test the SERS activity of the MPZS-Ag particles.The prepared MPZS-Ag particles possess both magnetic responsiveness and excellent SERS properties.SERS detection of different concentrations of melamine aqueous solutions and spiked milk samples were performed by the MPZS-Ag particles.The limit of detection(LOD) of the melamine in aqueous solutions was 10^-7 mol/L(0.0126 mg/L) and 0.6 mg/L in real milk samples using the MPZS-Ag particles as SERS substrates.The LOD of the melamine are much lower than the safety values of Food and Drug Administration and Codex Alimentarius Commission.These results indicate that the MPZS-Ag particles have promising application prospect for SERS analysis in food safety fields. | Ci Huang Feifei Lu Ke Xu Guanjun Ding Lijun You Jiabin Wang Qiqing Zhang | 2019 | Chinese Chemical Letters2019，30，12： | 6 |
| 10 | Synergistic graphene/aluminum surface plasmon coupling for zinc oxide lasing improvement显示文摘免费电子的集体摆动在材料的表面上产生电浆子。耳语画廊 microcavity 有效地从它的内部墙基于全部的思考在它的表面上限制轻地。当这些二种电磁波遇见对方时,多于在耳语画廊 microcavity 与 graphene 和艾尔 nanoparticles 的单层是涂的以后, 50 褶层和阀值被减少,从单个 ZnO microrod 的刺激排出物被提高。放射激光的表演的改进被归因于与 ZnO 激子 graphene/Al 表面电浆子联合的 synergistic 精力。放射激光的特征和联合机制系统地被调查。 | Qiuxiang Zhu Feifei Qin Junfeng Lu Zhu Zhu Haiyan Nan Zengliang Shi Zhenhua Ni Chunxiang Xu | 2017 | Nano Research2017，10，6： | 6 |
| 11 | Geochronology and geochemistry of mafic dykes in the Helanshan complex: Implications for Mesozoic tectonics in the North China Craton显示文摘The Helanshan tectonic belt(HTB) is a major tectonic divide between the Alxa and Ordos blocks in the North China Craton. The geochronology and petrogenesis of the mafic dykes in the northern HTB are keys to understanding the tectonic evolution of this belt. The mafic dykes, intruded into the Neoarchean-Paleoproterozoic metamorphic basement, are mainly composed of diabase with a mineral assemblage of plagioclase(45%-60%), pyroxene(25%-35%), minor quartz and Fe-Ti oxides. The LA-ICPMS U-Pb analysis of zircon grains from representative dykes yield a weighted mean age of 206 ± 1.9 Ma, which represents the crystallization age of the dyke. The diabases show high contents of Fe_2 O_3~T(11.88-17.55 wt.%), low contents of SiO_2(45.65-50.95 wt.%) and MgO(3.31-5.50 wt.%) with low Mg#(=100×MgO/(MgO + FeO) atomic ration) of 33-44. They are characterized by enrichment of light rare earth elements(LREEs) and large ion lithophile elements(LILEs)(e.g., Rb, Ba and Pb), and slight depletion of high field strength elements(HFSEs). These features suggest that the magma has undergone extensive fractionation of olivine and pyroxene but only minor crustal contamination during its evolution. Their high Sm contents and La/Sm ratios, and low Sm/Yb ratios indicate that magma from which the dykes formed was derived from low degree(about 5%) partial melting of an enriched garnet + spinel lherzolite mantle source. Together with regional geology, these geochemical and geochronological data suggest that the mafic dykes in the HTB were formed in an intracontinental extensional setting during the late Triassic. | Zhenghui Li Xiaoming Liu Yunpeng Dong M.Santosh Feifei Zhang Jie Xu | 2018 | Geoscience Frontiers2018，9，6： | 6 |
| 12 | Influence of Tourists′ Environmental Tropisms on Their Attitudes to Tourism and Nature Conservation in Natural Tourist Destinations: A Case Study of Jiuzhaigou National Park in China显示文摘People′s environmental tropism has an effect on their understanding of tourism-nature relationship,and also influences their attitudes to natural resources utilization and environmental protection.Taking Jiuzhaigou National Park of China as a study case,the authors conducted some quantitative analyses with the tools of SPSS 16.0 and LISREL 8.7,to explore the influence of tourists′ environmental tropism on their attitudes to tourism and nature conservation in the natural tourist destination.Three hypotheses and the theoretic model of influences of tourists′ environmental tropism on their attitudes to tourism and nature conservation have been tested and accepted based on the Structural Equation Model analysis on survey data collected in Jiuzhaigou National Park of China.Some conclusions were drawn as follows: 1) tourists′ human-prioritized concept influences their cognition to tourism-nature relationship.This concept has a positive influence on their supportive attitudes to tourism,but a negative influence on their attitudes to nature conservation;2) tourists′ human-nature coordination concept has a positive influence on their supportive attitudes to both tourism and nature conservation,especially to the latter.This paper generally proves that human′s environmental tropism does have an influence on their attitudes to tourism and nature conservation in natural tourist destinations. | CHENG Shaowen ZHANG Jie LU Shaojing XU Feifei ZHANG Honglei | 2011 | Chinese Geographical Science2011，21，3： | 5 |
| 13 | Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip显示文摘Genotyping platforms,as critical supports for genomics,genetics,and molecular breeding,have been well implemented at national institutions/universities in developed countries and multinational seed companies that possess high-throughput,automatic,large-scale,and shared facilities.In this study,we integrated an improved genotyping by target sequencing(GBTS)system with capture-in-solution(liquid chip)technology to develop a multiple single-nucleotide polymorphism(mSNP)approach in which mSNPs can be captured from a single amplicon.From one 40K maize mSNP panel,we developed three types of markers(40K mSNPs,251K SNPs,and 690K haplotypes),and generated multiple panels with various marker densities(1K–40K mSNPs)by sequencing at different depths.Comparative genetic diversity analysis was performed with genic versus intergenic markers and di-allelic SNPs versus non-typical SNPs.Compared with the one-amplicon-one-SNP system,mSNPs and within-mSNP haplotypes are more powerful for genetic diversity detection,linkage disequilibrium decay analysis,and genome-wide association studies.The technologies,protocols,and application scenarios developed for maize in this study will serve as a model for the development of mSNP arrays and highly efficient GBTS systems in animals,plants,and microorganisms. | Zifeng Guo Quannv Yang Feifei Huang Hongjian Zheng Zhiqin Sang Yanfen Xu Cong Zhang Kunsheng Wu Jiajun Tao Boddupalli MPrasanna Michael SOlsen Yunbo Wang Jianan Zhang Yunbi Xu | 2021 | Plant Communications2021，2，6： | 5 |
| 14 | Plasmon enhancement for Vernier coupled single-mode lasing from ZnO/Pt hybrid microcavities显示文摘开发单个模式操作并且改进放射激光以便保证 microlasers 和 nanolasers 的实际适用性的表演是必要的。在这份报纸,有 ZnO microcomb 的改变的 nanoscaled 空气差距的二六角形的 microteeth 被用来构造联合耳语画廊洞。这被做完成没有要求任何复杂或复杂的操作完成与 nanoscale 精确放,基于游标效果放射激光的一个稳定的单个模式。光获得和相应紫外放射激光表演通过结合磅 nanoparticles 的局部性的表面电浆子极大地被改进。ZnO/Pt 混合 microcavities 完成了单个模式与更高的方面模式抑制比率和更低的阀值放射激光的紧张的七倍的改进。导致了这改进的机制详细被描述了。 | Yueyue Wang Feifei Qin Junfeng Lu Jitao Li Zhu Zhu Qiuxiang Zhu Ye Zhu Zengliang Shi Chunxiang Xu | 2017 | Nano Research2017，10，10： | 5 |
| 15 | Microwave photonics: radio-over-fiber links, systems, and applications [Invited]显示文摘Microwave photonics(MWPs)uses the strength of photonic techniques to generate,process,control,and distribute microwave signals,combining the advantages of microwaves and photonics.As one of the main topics of MWP,radio-over-fiber(RoF)links can provide features that are very difficult or even impossible to achieve with traditional technologies.Meanwhile,a considerable number of signal-processing subsystems have been carried out in the field of MWP as they are instrumental for the implementation of many functionalities.However,there are still several challenges in strengthening the performance of the technology to support systems and applications with more complex structures,multiple functionality,larger bandwidth,and larger processing capability.In this paper,we identify some of the notable challenges in MWP and review our recent work.Applications and future direction of research are also discussed. | Kun Xu Ruixin Wang Yitang Dai Feifei Yin Jianqiang Li Yuefeng Ji Jintong Lin | 2014 | Photonics Research2014，2，4： | 4 |
| 16 | Trust-Based Context-Aware Mobile Social Network Service Recommendation显示文摘The service recommendation mechanism as a key enabling technology that provides users with more proactive and personalized service is one of the important research topics in mobile social network(MSN). Meanwhile,MSN is susceptible to various types of anonymous information or hacker actions. Trust can reduce the risk of interaction with unknown entities and prevent malicious attacks. In our paper,we present a trust-based service recommendation algorithm in MSN that considers users' similarity and friends' familiarity when computing trustworthy neighbors of target users. Firstly,we use the context information and the number of co-rated items to define users' similarity. Then,motivated by the theory of six degrees of space,the friend familiarity is derived by graph-based method. Thus the proposed methods are further enhanced by considering users' context in the recommendation phase. Finally,a set of simulations are conducted to evaluate the accuracy of the algorithm. The results show that the friend familiarity and user similarity can effectively improve the recommendation performance,and the friend familiarity contributes more than the user similarity. | XU Jun ZHONG Yuansheng ZHU Wenqiang SUN Feifei | 2017 | Wuhan University Journal of Natural Sciences2017，22，2： | 4 |
| 17 | Plasmon-enhanced ZnO whispering-gallery mode lasing显示文摘 | Chunxiang Xu Feifei Qin Qiuxiang Zhu Junfeng Lu Yueyue Wang Jitao Li Yi Lin Qiannan Cui Zengliang Shi Arumugam Gowri Manohari | 2018 | Nano Research2018，11，6： | 4 |
| 18 | Effect of Li+ ion concentration on upconversion emission and temperature sensing behavior of La2O3:Er3+ phosphors显示文摘The effects of Li^+ co-doping concentration on the structure, upconversion luminescence and temperature sensing behavior of Er^(3+):La_2O_3 phosphors were investigated. X-ray diffraction and scanning electron microscopy observations reveal that Li^+ ion co-doping can change the lattice parameter of La_2O_3 host and increase the particle size of the samples. The optical investigation shows that co-doping of Li^+ ions can enhance the upconversion emission of Er^(3+) ions in La_2O_3 matrix effectively. Most importantly, the temperature sensing sensitivity of the samples is found to be dependent on Li^+ co-doping concentration,when the emission intensity ratio of the(~2H_(11/2)→~4 I_(15/2)) and(~4 S_(3/2)→~4 I_(15/2)) transitions of Er^(3+) is chosen as the thermometric index. Both of the optimum upconversion luminescence and temperature sensing sensitivity are obtained for 7 mol% Li^+ co-doped sample. When the Li^+ concentration is beyond 7 mol%,both the quenching in upconversion intensity and the degradation of temperature sensitivity are observed, which may be due to the serious distortion in local crystal field around Er^(3+) ions caused by the excess Li^+ ions. | Guangrun Chen Ruoshan Lei Shiqing Xu Huanping Wang Shilong Zhao Feifei Huang Yin Tian | 2018 | Journal of Rare Earths2018，36，2： | 4 |
| 19 | Sequential infection with H1N1 and SARS-CoV-2 aggravated COVID-19 pathogenesis in a mammalian model, and covaccination as an effective method of prevention of COVID-19 and influenza显示文摘Influenza A virus may circulate simultaneously with the SARS-CoV-2 virus,leading to more serious respiratory diseases during this winter.However,the influence of these viruses on disease outcome when both influenza A and SARS-CoV-2 are present in the host remains unclear.Using a mammalian model,sequential infection was performed in ferrets and in K18-MCE2 mice,with SARS-CoV-2 infection following H1N1.We found that co-infection with H1N1 and SARS-CoV-2 extended the duration of clinical manifestation of COVID-19,and enhanced pulmonary damage,but reduced viral shedding of throat swabs and viral loads in the lungs of ferrets.Moreover,mortality was increased in sequentially infected mice compared with single-infection mice.Compared with singlevaccine inoculation,co-inoculation of PiCoVacc(a SARS-CoV-2 vaccine)and the flu vaccine showed no significant differences in neutralizing antibody titers or virus-specific immune responses.Combined immunization effectively protected K18-MCE2 mice against both H1N1 and SARS-CoV-2 infection.Our findings indicated the development of systematic models of co-infection of H1N1 and SARS-CoV-2,which together notably enhanced pneumonia in ferrets and mice,as well as demonstrated that simultaneous vaccination against HINT and SARS-CoV-2 may be an effective prevention strategy for the coming winter. | Linlin Bao Wei Deng Feifei Qi Qi Lv Zhiqi Song Jiangning Liu Hong Gao Qiang Wei Pin Yu Yanfeng Xu Yajin Qu Fengdi Li Jing Xue Shuran Gong Mingya Liu Guanpeng Wang Shunyi Wang Binbin Zhao Bin Cong Chuan Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 4 |
| 20 | SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice显示文摘Background:Cardiovascular diseases(CVDs)and diabetes mellitus(DM)are top two chronic comorbidities that increase the severity and mortality of COVID-19.However,how SARS-CoV-2 alters the progression of chronic diseases remain unclear.Methods:We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in mice.SARS-CoV-2’s impacts on pathogenesis of chronic diseases were studied through histopathological,virologic and molecular biology analysis.Results:Pre-existing CVDs resulted in viral invasion,ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 infection.Viral infection increased fasting blood glucose and reduced insulin response in DM model.Bone mineral density decreased shortly after infection,which associated with impaired PI3K/AKT/mTOR signaling.Conclusion:We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic diseases.Pre-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection,which further aggravated the pre-existing diseases.This work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection. | Yuanwu Ma Dan Lu Linlin Bao Yajin Qu Jiangning Liu Xiaolong Qi Lei Yu Xu Zhang Feifei Qi Qi Lv Yunpeng Liu Xudong Shi Caixian Sun Jing Li Jie Wang Yunlin Han Kai Gao Wei Dong Ning Liu Shan Gao Jing Xue Qiang Wei Sidan Pan Hong Gao Lianfeng Zhang Chuan Qin | 2021 | Animal Models and Experimental Medicine2021，4，1： | 3 |
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