|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Apoptosis during β-mercaptoethanol-induced differentiation of adult adipose-derived stromal cells into neurons显示文摘β-mercaptoethanol can induce adipose-derived stromal cells to rapidly and efficiently differentiate into neurons in vitro.However,because of the short survival time of the differentiated cells,clinical applications for this technique are limited.As such,we examined apoptosis of neurons differentiated from adipose-derived stromal cells induced with β-mercaptoethanol in vitro using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and transmission electron microscopy.The results revealed that the number of surviving cells decreased and apoptosis rate increased as induction time extended.Taken together,these results suggest that apoptosis occurring in the process of adipose-derived stromal cells differentiating into neurons is the main cause of cell death.However,the mechanism underlying cellular apoptosis should be researched further to develop methods of controlling apoptosis for clinical applications. | Yanan Cai Xiaodong Yuan Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 19 |
| 2 | A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes显示文摘The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves.After induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic morphology.The cell quantity gradually decreased with prolonged induction time.Nestin,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not expressed.These results suggest that the induced astrocytes reached their peak at 14 days.Further optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time. | Ya Ou Xiaodong Yuan Yanan Cai Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 12 |
| 3 | Effect of warming Yang and removing blood stasis method on matrix metalloproteinases/tissue inhibitor metalloproteinases levels secreted by cultured endometrial cells from patients with endometriosis显示文摘OBJECTIVE:To investigate the effect of Chinese medicines using the warming Yang and removing blood stasis method on levels of matrix metalloproteinases(MMPs)/tissue inhibitor metalloproteinases(TIMPs) secreted by cultured endometrial cells from patients with endometriosis.METHODS:Ectopic and eutopic endometrial cells obtaind from 15 endometriosis patients were cultured in vitro,and divided randomly into five groups:high dose;moderate dose;low dose;nemestran;blank control.The three dose groups were treated with a decoction prepared according to the principle of warming Yang and removing blood stasis;nemestran and 0.9%NaCI were administered to the nemestran group and balnk control group,respectively.Eutopic endometrial cells obtaind from 10 hysteromyoma patients were cultured in vitro,as the normal control group,0.9%NaCI were administered to the normal control group.Cell culture supernatants were collected and levels of matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9),tissue inhibitor metalloproteinase-1(TIMP-1) and tissue inhibitor metalloproteinase-2(TIMP-2) detected by enzyme-linked immuno sorbent assay(ELISA).RESULTS:Compared with the normal control group,levels of MMP-1,MMP-2,and MMP-9 in eutopic and ectopic endometrium cell supernatants in the blank control group were increased,whereas levels of TIMP-1 and TIMP-2 were decreased(P <0.05).Compared with the blank control group,levels of MMP-1 and MMP-2 in ectopic and eutopic endometrium cell supernatants cultured in low-dose,middle-dose,and high-dose groups were decreased,whereas levels of TIMP-1 and TIMP-2 were increased significantly(P < 0.05).CONCLUSION:The warming Yang and removing blood stasis method affects expression of MMPs andTIMPs. | Huang Yanhui Shen Lin Cai Anhe Xiao Jing | 2015 | Journal of Traditional Chinese Medicine2015，35，5： | 10 |
| 4 | Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions显示文摘β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K+ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions. | Xiaodong Yuan Yanan Cai Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，34： | 8 |
| 5 | Autophagy and apoptosis during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro显示文摘β-mercaptoethanol can induce adult adipose-derived stromal cells to rapidly and efficiently differentiate into typical neuron-like cells in vitro.Immunohistochemistry showed that neuron specific enolase and neurofilament-200 expression gradually increased with the extension of induction time,and peaked at 5 hours.By contrast,glial fibrillary acidic protein was negatively expressed at all time points.Induced cells possessed a typical Nissl body,apoptosis showing condensed chromatin in the nucleus,autophagosomes with a bilayered membrane and autolysosomes in the cytoplasm at 5 hours.TUNEL assay and immunohistochemistry and immunofluorescence demonstrated that apoptosis and caspase-3 expression increased and peaked at 8 hours.Immunohistochemistry and immunofluorescence showed that microtubule-associated protein light chain 3 gradually increased with induction and reached a peak at 5 hours.These results indicate that autophagy played an important role in protecting cells during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro. | Yanhui Lu Xiaodong Yuan Ya Ou Yanan Cai Shujuan Wang Qiaoyu Sun Wenli Zhang | 2012 | Neural Regeneration Research2012，7，16： | 5 |
| 6 | The trend of the APRGP velocity field and plates movement derived from GPS data显示文摘High-precision data processing theories and methods for the wide area GPS network–––Asia Pacific Regional Geodesy Project (APRGP), are systematically discussed in this article. With the software bernese5.0 update, the annual results of APRGP coordinate, velocity field and the trend of plate movement are reestimated, and the corresponding accuracy of adjustment with different constraints, also the velocity field of APRGP, are presented and analyzed. Conclusions could be drawn that Eurasian plate (EURA) rotates clockwise, the Australian plate (AUST) and the Indian plate (INDI) move from southwest to northeast, while the Pacific plate (PCFC) is drifting to northwest, which shows agreement with those of IGS results. | CHENG PengFei , WANG Hua, CHENG YingYan, WEN HanJiang & CAI YanHui Chinese Academy of Surveying and Mapping, Beijing 100039, China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，4： | 5 |
| 7 | Aerodynamic configuration integration design of hypersonic cruise aircraft with inward-turning inlets显示文摘In this work, a novel airframe/propulsion integration design method of the wing-body configuration for hypersonic cruise aircraft is proposed, where the configuration is integrated with inward-turning inlets. With the help of this method, the major design concern of balancing the aerodynamic performance against the requirements for efficient propulsion can be well addressed. A novel geometric parametrically modelling method based on a combination of patched class and shape transition(CST) and COONs surface is proposed to represent the configuration, especially a complex configuration with an irregular inlet lip shape. The modelling method enlarges the design space of components on the premise of guaranteeing the configuration integrity via special constraints imposed on the interface across adjacent surfaces. A basic flow inside a cone shaped by a dual-inflection-point generatrix is optimized to generate the inward-turning inlet with improvements of both compression efficiency and flow uniformity. The performance improvement mechanism of this basic flow is the compression velocity variation induced by the variation of the generatrix slope along the flow path. At the design point, numerical simulation results show that the lift-to-drag ratio of the configuration is as high as 5.2 and the inlet works well with a high level of compression efficiency and flow uniformity. The design result also has a good performance on off-design conditions. The achievement of all the design targets turns out that the integration design method proposed in this paper is efficient and practical. | Jifei WANG Jinsheng CAI Chuanzhen LIU Yanhui DUAN Yaojie YU | 2017 | Chinese Journal of Aeronautics2017，30，4： | 5 |
| 8 | Overexpression of miR-101 suppresses collagen synthesis by targeting EZH2 in hypertrophic scar fibroblasts显示文摘Background:MicroRNA-101(miR-101)is a tumor suppressor microRNA(miRNA)and its loss is associated with the occurrence and progression of various diseases.However,the biological function and target of miR-101 in the pathogenesis of hypertrophic scars(HS)remains unknown.Methods:We harvested HS and paired normal skin(NS)tissue samples from patients and cultured their fibroblasts(HSF and NSF,respectively).We used quantitative reverse transcriptase polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),enzyme-linked immunosorbent assays(ELISA)and Western blot analyses to measure mRNA levels and protein expression of miR-101,enhancer of zeste homolog 2(EZH2),collagen 1 and 3(Col1 and Col3)andα-smooth muscle actin(α-SMA)in different in vitro conditions.We also used RNA sequencing to evaluate the relevant signaling pathways and bioinformatics analysis and dual-luciferase reporter assays to predict miR-101 targets.We utilized a bleomycin-induced fibrosis mouse model in which we injected miR-101 mimics to evaluate collagen deposition in vivo.Results:We found low expression of miR-101 in HS and HSF compared to NS and NSF.Overexpressing miR-101 decreased Col1,Col3 andα-SMA expression in HSF.We detected high expression of EZH2 in HS and HSF.Knockdown of EZH2 decreased Col1,Col3 andα-SMA in HSF.Mechanistically,miR-101 targeted the 3-untranslated region(3UTR)of EZH2,as indicated by the decreased expression of EZH2.Overexpressing EZH2 rescued miR-101-induced collagen repression.MiR-101 mimics effectively suppressed collagen deposition in the bleomycin-induced fibrosis mouse model.Conclusions:Our data reveal that miR-101 targets EZH2 in HS collagen production,providing new insight into the pathological mechanisms underlying HS formation. | Jie Li Yan Li Yunchuan Wang Xiang He Jing Wang Weixia Cai Yanhui Jia Dan Xiao Jian Zhang Ming Zhao Kuo Shen Zichao Li Wenbin Jia Kejia Wang Yue Zhang Linlin Su Huayu Zhu Dahai Hu | 2021 | Burns & Trauma2021，9，1： | 3 |
| 9 | The Notch pathway attenuates burn-induced acute lung injury in rats by repressing reactive oxygen species显示文摘Background:Acute lung injury(ALI)is a common complication following severe burns.The underlying mechanisms of ALI are incompletely understood;thus,available treatments are not sufficient to repair the lung tissue after ALI.Methods:To investigate the relationship between the Notch pathway and burn-induced lung injury,we established a rat burn injury model by scalding and verified lung injury via lung injury evaluations,including hematoxylin and eosin(H&E)staining,lung injury scoring,bronchoalveolar lavage fluid and wet/dry ratio analyses,myeloperoxidase immunohistochemical staining and reac-tive oxygen species(ROS)accumulation analysis.To explore whether burn injury affects Notch1 expression,we detected the expression of Notch1 and Hes1 after burn injury.Then,we extracted pulmonary microvascular endothelial cells(PMVECs)and conducted Notch pathway inhibition and activation experiments,via aγ-secretase inhibitor(GSI)and OP9-DLL1 coculture,respectively,to verify the regulatory effect of the Notch pathway on ROS accumulation and apoptosis in burn-serum-stimulated PMVECs.To investigate the regulatory effect of the Notch pathway on ROS accumulation,we detected the expression of oxidative-stress-related molecules such as superoxide dismutase,nicotinamide adenine dinucleotide phosphate(NADPH)oxidase(NOX)2,NOX4 and cleaved caspase-3.NOX4-specific small interfering RNA(siRNA)and the inhibitor GKT137831 were used to verify the regulatory effect of the Notch pathway on ROS via NOX4.Results:We successfully established a burn model and revealed that lung injury,excessive ROS accumulation and an inflammatory response occurred.Notch1 detection showed that the expression of Notch1 was significantly increased after burn injury.In PMVECs challenged with burn serum,ROS and cell death were elevated.Moreover,when the Notch pathway was suppressed by GSI,ROS and cell apoptosis levels were significantly increased.Conversely,these parameters were reduced when the Notch pathway was activated by OP9-DLL1.Mechanistically,the inhibition of NOX4 by siRNA and GKT137831 showed that the Notch pathway reduced ROS production and cell apoptosis by downregulating the expression of NOX4 in PMVECs.Conclusions:The Notch pathway reduced ROS production and apoptosis by downregulating the expression of NOX4 in burn-stimulated PMVECs.The Notch-NOX4 pathway may be a novel therapeutic target to treat burn-induced ALI. | Weixia Cai Kuo Shen Peng Ji Yanhui Jia Shichao Han Wanfu Zhang Xiaolong Hu Xuekang Yang Juntao Han Dahai Hu | 2022 | Burns & Trauma2022，10，1： | 2 |
| 10 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 11 | Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants显示文摘 | Yanhui Peng Wuling Lin Weiming Cai Rajeev Arora | 2007 | Planta2007，，3： | 1 |
| 12 | Lactate:a prospective target for therapeutic intervention in psychiatric disease显示文摘Although antipsychotics that act via monoaminergic neurotransmitter modulation have considera ble therapeutic effect,they cannot completely relieve clinical symptoms in patients suffering from psychiatric disorde rs.This may be attributed to the limited range of neurotransmitters that are regulated by psychotropic drugs.Recent findings indicate the need for investigation of psychotropic medications that target less-studied neurotransmitte rs.Among these candidate neurotransmitters,lactate is developing from being a waste metabolite to a glial-neuronal signaling molecule in recent years.Previous studies have suggested that cerebral lactate levels change considerably in numerous psychiatric illnesses;animal experiments have also shown that the supply of exogenous la ctate exerts an antidepressant effect.In this review,we have described how medications targeting newer neurotransmitte rs offer promise in psychiatric diseases;we have also summarized the advances in the use of lactate(and its corresponding signaling pathways)as a signaling molecule.In addition,we have described the alterations in brain lactate levels in depression,anxiety,bipolar disorder,and schizophrenia and have indicated the challenges that need to be overcome before brain lactate can be used as a therapeutic target in psychopharmacology. | Yanhui Cai Haiyun Guo Tianle Han Huaning Wang | 2024 | Neural Regeneration Research2024，19，7： | 0 |
| 13 | AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple(Ananas comosus)显示文摘Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is poorly understood. Here, we studied the pineapple WRKY gene, AcWRKY28, by generating AcWRKY28 over-expressing transgenic pineapple plants. Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that “plant-pathogen interaction” pathway genes, including 9calcium-dependent protein kinases (CPKs), were up-regulated in AcWRKY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one-hybrid assays revealed AcCPK12, AcCPK3, AcCPK8, AcCPK1, and AcCPK15 as direct targets of AcWRKY28. Consistently, the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt, drought, and disease resistance. This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes. | Qiao Zhou Samaranayaka Vidana Gamage Nirosha Priyadarshani Rongjuan Qin Han Cheng Tiantian Luo Myat Hnin Wai Mohammad Aqa Mohammadi Yanhui Liu Chang Liu Hanyang Cai Xiaomei Wang Yeqiang Liu Yuan Qin Lulu Wang | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 14 | Catalytic Conversion of C_(1) Molecules on Atomically Precise Metal Nanoclusters显示文摘Metal nanoclusters with accurate compositions and precise crystalline structures hold remarkable attention in serving as a unique model catalyst for well-defined correlations between structure and catalytic activity.More importantly,these metal nanoclusters exhibit strong quantum confinement effects,which differ from their larger nanoparticles in a number of catalytic reactions.This review focuses on recent advances of atomically precise metal nanoclusters for C_(1) compound conversion(C_(O),CO_(2),CH_(4),and HCOOH),including thermally-driven catalysis,photocatalysis,and electrocatalysis.The reaction mechanisms are discussed at an atomic-or even electron-level.It is anticipated that the progress in this research area could be extended to catalytic applications of metal nanoclusters in C_(1) chemistry. | Dan Yang Yongnan Sun Xiao Cai Weigang Hu Yihu Dai Yan Zhu Yanhui Yang | 2022 | CCS Chemistry2022，4，1： | 0 |
| 15 | Combined Effect of Outdoor Time and Other Modifiable Factors on Myopia Incidence Among Children and Adolescents—9 PLADs,China,2020显示文摘Summary What is already known about this topic?Myopia has been identified as a significant emerging challenge and policy priority among children and adolescents in China by the Ministry of Education and seven other departments.Limited research has been conducted to investigate the collective impact of outdoor time and other modifiable factors on the incidence of myopia. | Panliang Zhong Yunfei Liu Ning Ma Jiajia Dang Di Shi Shan Cai Ziyue Chen Yanhui Dong Peijin Hu Jun Ma Patrick W.C Lau Randall S.Stafford Yi Song | 2024 | China CDC weekly2024，6，9： | 0 |
| 16 | Safety and feasibility of laparoscopic surgery for colorectal and gastric cancer under the Chinese multi-site practice policy:admittance standards of competence are needed显示文摘Background:The multi-site practice(MSP)policy has been practiced in China over 10 years.This study aimed to investigate the safety and feasibility of performing laparoscopic surgery for colorectal cancer(LSCRC)and gastric cancer(LSGC)under the Chinese MSP policy.Methods:We collected and analysed the data from 1,081 patients who underwent LSCRC or LSGC performed by one gastrointestinal surgeon in his original hospital(n=573)and his MSP institutions(n=508)between January 2017 and December 2020.Baseline demographics,intraoperative outcomes,post-operative recovery,and pathological results were compared between the original hospital and MSP institutions,as well as between MSP institutions with and without specific competence(surgical skill,operative instrument,perioperative multi-discipline team).Results:In our study,690 patients underwent LSCRC and 391 patients underwent LSGC.The prevalence of post-operative complications was comparable for LSCRC(11.5%vs 11.1%,P=0.89)or LSGC(15.2%vs 12.6%,P=0.46)between the original hospital and MSP institutions.However,patients in MSP institutions without qualified surgical assistant(s)and adequate instruments experienced longer operative time and greater intraoperative blood loss.The proportion of patients with inadequate lymph-node yield was significantly higher in MSP institutions than in the original hospital for both LSCRC(11.5%vs 21.2%,P<0.01)and LSGC(9.8%vs 20.5%,P<0.01).Conclusion:For an experienced gastrointestinal surgeon,performing LSCRC and LSGC outside his original hospital under the MSP policy is safe and feasible,but relies on the precondition that the MSP institutions are equipped with qualified surgical skills,adequate operative instruments,and complete perioperative management. | Zhenghao Cai Haiqin Song Zhenfeng Huang Abraham Fingerhut Ximo Xu Hao Zhong Zhigang Li Yingjie Zhang Dachong Sha Dandan Bao Haibo Wang Binghua Cai Shangbo Hua Yanhui Zhang Jianguang Sun Ke Ye Jianwen Li Yong Lu Bo Feng | 2022 | Gastroenterology Report2022，10，1： | 0 |
| 17 | Dietary Pterostilbene Inhibited Colonic Inflammation in Dextran-Sodium-Sulfate-Treated Mice:A Perspective of Gut Microbiota显示文摘Dietary interventions based on the use of bioactive nutraceuticals might offer an effective adjuvant therapeutic and preventive method for inflammatory bowel disease by reshaping colitis-associated bacterial dysbiosis.The current study aimed to determine the antiinflammatory effect of pterostilbene(PTE,a methylated derivative of resveratrol)and its potential modulatory roles in gut microbiota in a dextran sodium sulfate(DSS)-induced colitis mouse model.Our results supported our hypothesis that dietary PTE exerted protective effects against colonic inflammation;evidenced by the reduced colonic tissue damage,decreased disease activity index,and lowered production of pro-inflammatory cytokines such as interferon gamma,interleukin(IL)-2,IL-4,and IL-6 in the colon of DSS-treated mice.Moreover,a-diversity analysis indicated that dietary PTE significantly improved gut microbial evenness and diversity.Noteworthy,PTE modified gut microbiota composition toward a healthier profile by boosting the richness of Bifidobacterium and decreasing the distribution of pathogenic Bilophila and Rc4-4.Pearson correlation analysis also revealed strong associations between the shifting of gut microbiota and expression of inflammatory cytokines in the colon.Overall,our study demonstrated that dietary PTE alleviated the severity of colitis in DSS-treated mice and gut microbiota may play an indispensable role in this process mechanistically. | Fang Li Qi Wang Yanhui Han Mingyue Song Xiaokun Cai Timothy Goulette Hang Xiao | 2021 | Infectious Microbes & Diseases2021，3，1： | 0 |
| 18 | Development levels of pediatric palliative care teams and the extent of palliative care understanding and implementation among pediatric oncologists in China显示文摘Importance:Pediatric palliative care(PPC)is an interdisciplinary collaboration that focuses on the prevention and relief of patient suffering.PPC has emerged as a critical field of medical expertise and practice.However,no information is available regarding the progress of PPC in the Chinese mainland.Objective:This study investigated the geographic distribution,team structure,and services of PPC teams in the Chinese mainland.It also investigated the level of understanding and implementation among pediatric oncologists regarding PPC.Methods:The PPC subspecialty group of the Pediatrics Society of the Chinese Medical Association included 45 PPC teams.The team structure and services were investigated using questionnaires mailed to the team leader of each PPC team.In addition,we sent questionnaires regarding the level of PPC understanding and implementation of PPC practices to 170 pediatric oncologists in 11 hospitals.Results:The geographical distribution of PPC teams is uneven in China.Most PPC teams are concentrated in the eastern provincial capital of China.Most PPC teams had limited staff and services.The level of PPC understanding was considerably limited across all demographics;most pediatric oncologists reported“some understanding”(n=71,41.8%)or“poor understanding”(n=50,29.4%).Only 62.9%of pediatric oncologists had experience providing advice to family members regarding PPC matters.Interpretation:China is currently experiencing a critical shortage of PPC resources.Most pediatric oncologists had a limited understanding of PPC and reported limited practical implementation of PPC,which leads to underutilization of PPC resources. | Siyu Cai Qiaohong Guo Xianjing Wang Ruixin Wang Peiyi Yang Yuchen Zhou Yanhui Luo Xuan Zhou Xiaoxia Peng | 2021 | Pediatric Investigation2021，5，4： | 0 |
| 19 | Epigenetic modification mechanisms of chloroplasts mutants in pineapple leaves during somatic regeneration显示文摘Somaclonal variation in tissue culture is a common phenomenon induced by various external or internal environmental conditions,resulting in heritable or non-heritable alterations in gene expression. One crucial mechanism involved in plant growth and development is epigenetic regulation. A highly dynamic epigenome can respond to environmental changes by regulating gene expression. DNA methylation is one of these epigenetic modifications that can alter gene expression in tissue-cultured pineapple plants. The underlying mechanism of such somaclonal variations in pineapple and the epigenetic regulation involvement in somaclonal variations has not been studied. This study performed DNA methylome and transcriptome sequencing of wild-type(WT) and mutant pineapple plants(WS, HW, and TW). We observed altered DNA methylation patterns in chlorophyll development in the mutants. Specifically, we noticed that the methylation levels in the CHG and CHH contexts were lower in the gene body regions compared to the upstream and downstream regions. We identified several thousand differentially methylated regions(DMRs) located at the gene body regions, some of which overlapped with the differentially expressed genes(DEGs). Functional enrichment analyses suggested that these genes were involved in chlorophyll metabolism. Thus, our results revealed that the transcriptional regulation of many chlorophyll metabolic essential genes could be regulated by DNA methylation caused by somaclonal variations and provided insights into epigenetic mechanisms underlying the regulation of chlorosis in pineapple plants. | Yanhui Liu S.V.G.N.Priyadarshani Meirong Chi Maokai Yan Mohammad Aqa Mohammadi Man Zhang Qiao Zhou Lulu Wang Tiantian Luo Myat Hnin Wai Xiaomei Wang Hanyang Cai Haifeng Wang Yuan Qin | 2023 | Horticultural Plant Journal2023，9，3： | 0 |
| 20 | Characterization of germline development and identification of genes associated with germline specification in pineapple显示文摘Understanding germline specification in plants could be advantageous for agricultural applications.In recent decades,substantial efforts have been made to understand germline speci fication in several plant species,including Arabidopsis,rice,and maize.However,our knowledge of germline speci fication in many agronomically important plant species remains obscure.Here,we characterized the female germline speci fication and subsequent female gametophyte development in pineapple using callose staining,cytological,and whole-mount immunolocalization analyses.We also determined the male germline speci fication and gametophyte developmental timeline and observed male meiotic behavior using chromosome spreading assays.Furthermore,we identi fied 229 genes that are preferentially expressed at the megaspore mother cell(MMC)stage during ovule development and 478 genes that are preferentially expressed at the pollen mother cell(PMC)stage of anther development using comparative transcriptomic analysis.The biological functions,associated regulatory pathways and expression patterns of these genes were also analyzed.Our study provides a convenient cytological reference for exploring pineapple germline development and a molecular basis for the future functional analysis of germline speci fi cation in related plant species. | Lihua Zhao Liping Liu Yanhui Liu Xianying Dou Hanyang Cai Mohammad Aslam Zhimin Hou Xingyue Jin Yi Li Lulu Wang Heming Zhao Xiaomei Wang Adrien Sicard Yuan Qin | 2021 | Horticulture Research2021，8，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员