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| 1 | Zircon U-Pb dating confirms existence of a Caledonian scheelite-bearing aplitic vein in the Penggongmiao granite batholith, South Hunan显示文摘Granite at Penggongmiao is a large batholith in the Nanling Range, with an outcrop area of over 900 km 2 . There are many scheelite-quartz veins around the granite. LA-ICP-MS U-Pb dating was carried out for zircons from the granite. The middlecoarse-grained biotite granite has U-Pb ages of 435 to 436 Ma. Ages of 426.5±2.5 Ma were obtained for aplitic dyke cross-cutting the granite. The scheelite of magmatic origin in the aplite dyke was identified from petrographic investigation. This demonstrates that W-bearing granites of Early Paleozoic (corresponding to the Caledonian orogensis in the traditional sense) occur in the Nanling Range. This finding has important implications for the ore-forming potential of Paleozoic granites and on the extent of Mesozoic mineralization. Thus it merits performing an intensive study of Paleozoic granites in South China. The occurrences of aplite or microgranite may be an indicative of the Caledonian tungsten granites and associated W mineralization. | ZHANG WenLan WANG RuCheng LEI ZeHeng HUA RenMin ZHU JinChu LU JianJun XIE Lei CHE XueDong ZHANG RongQing YAO Yuan CHEN Jun | 2011 | Chinese Science Bulletin2011，56，19： | 23 |
| 2 | Activation of sonic hedgehog signaling pathway is an independent potential prognosis predictor in human hepatocellular carcinoma patients显示文摘Objective:The activation of hedgehog(HH) pathway is implicated in the development of human malignancies including hepatocellular carcinoma(HCC).However,the clinical impact of HH activation in HCC patients is still unclear.This study was conducted to confirm whether the expression of HH pathway components was associated with HCC progression and clinical outcome.Methods:This study was a sample-expanded and prolonged follow up of one of our previous studies.It included 46 HCC patients who underwent surgical treatment from 2002 to 2005.The expression of sonic HH(SHH),patched-1(PTCH1),smoothened(SMOH) and glioma-associated oncogene-1(GLI1) genes in tumor and adjacent normal tissues extracted from the patients were examined by reverse transcriptionpolymerase chain reaction(RT-PCR) to explore the relationship between these genes and the clinical prognosis of HCC.Results:The expression levels of SHH,PTCH1,SMOH and GLI1 in HCC tissues were 60.87%,50.00%,32.61% and 54.35%,respectively.The expression levels of SHH-related molecules were relatively intense in cancer tissue,but insignificantly correlated with any clinicopathological factors of tumor.Transcriptional factor GLI1 was the only molecule associated with poor prognosis among the HCC patients.The expression of GLI1 gene in tumor tissues was significantly related with disease-free survival(DFS)(P=0.042) and overall survival(OS)(P=0.030).The simultaneous expression of GLI1 in tumor and adjacent normal liver tissues correlated with DFS(P<0.029) and OS(P<0.025).Conclusions:HH signaling activation is an important event in the development of human HCC.The expression of GLI1 in SHH pathway is possibly involved in HCC progression,which may be a useful prognostic indicator of HCC. | Li Che Yan-Hua Yuan Jun Jia Jun Ren | 2012 | Chinese Journal of Cancer Research2012，24，4： | 15 |
| 3 | Review grain yield and nitrogen use efficiency in rice production regions in China显示文摘As one of the staple food crops, rice(Oryza sativa L.) is widely cultivated across China, which plays a critical role in guaranteeing national food security. Most previous studies on grain yield or/and nitrogen use efficiency(NUE) of rice in China often involved site-specific field experiments, or small regions with insufficient data, which limited the representation for the current rice production regions. In this study, a database covering a wide range of climate conditions, soil types and field managements across China, was developed to estimate rice grain yield and NUE in various rice production regions in China and to evaluate the relationships between N rates and grain yield, NUE. According to the database for rice, the values of grain yield, plant N accumulation, N harvest index(HIN), indigenous N supply(INS), internal N efficiency(IE_N), reciprocal internal N efficiency(RIE_N), agronomic N use efficiency(AE_N), partial N factor productivity(PEPN), physiological N efficiency(PE_N), and recover efficiency of applied N(RE_N) averaged 7.69 t ha^(–1), 152 kg ha^(–1), 0.64 kg kg^(–1), 94.1 kg kg^(–1), 53.9 kg kg^(–1), 1.98 kg kg^(–1), 12.6 kg kg^(–1), 48.6 kg kg^(–1), 33.8 kg kg^(–1), and 39.3%, respectively. However, the corresponding values all varied tremendously with large variation. Rice planting regions and N rates had significant influence on grain yield, N uptake and NUE values. Considering all observations, N rates of 200 to 250 kg ha^(–1) commonly achieved higher rice grain yield compared to less than 200 kg N ha^(–1) and more than 250 kg N ha^(–1) at most rice planting regions. At N rates of 200 to 250 kg ha^(–1), significant positive linear relationships were observed between rice grain yield and AE_N, PE_N, RE_N, IE_N, and PFPN, and 46.49, 24.64, 7.94, 17.84, and 88.24% of the variation in AE_N, PE_N, RE_N, IE_N, and PFPN could be explained by grain yield, respectively. In conclusion, in a reasonable range of N application, an increase in grain yield can be achieved accompanying by an acceptable NUE. | CHE Sheng-guo ZHAO Bing-qiang LI Yan-ting YUAN Liang LI Wei LIN Zhi-an HU Shu-wen SHEN Bing | 2015 | Journal of Integrative Agriculture2015，14，12： | 12 |
| 4 | Red River barrier and Pleistocene climatic fluctuations shaped the genetic structure of Microhyla fissipes complex (Anura: Microhylidae) in southern China and Indochina显示文摘 | Zhi-Yong YUAN Chatmongkon SUWANNAPOOM Fang YAN Nikolay A. POYARKOV JR Sang Ngoc NGUYEN Hong-man CHEN Siriwadee CHOMDEJ Robert W, MURPHY Jing CHE | 2016 | Current Zoology2016，62，6： | 11 |
| 5 | Body mass index does not affect the survival of pancreatic cancer patients显示文摘AIM to evaluate the association of body mass index(b MI) with the overall survival of pancreatic ductal adenocarcinoma(PDAC) patients.METHODS A retrospective analysis of PDAC patients diagnosed in the National Cancer Center of China between January 1999 and December 2014 was performed. these patients were categorized into four b MI groups(< 18.5, 18.5-22.9, 23-27.4 and ≥ 27.5 kg/m2). χ2 tests for comparison of the proportions of categorical variables, and Student's t-test or Mann-Whitney test for continuous variables were employed. Survival analysis was performed with the Kaplan-Meyer method. their HRs of mortality and 95%CIs were estimated using the Cox proportional hazards model.RESULTS With a median age of 59.6 years(range: 22.5-84.6 years), in total 1783 PDAC patients were enrolled in this study. their mean usual b MI was 24.19 ± 3.53 for the whole cohort. More than half of the patients(59.3%) experienced weight loss during the disease onset and progression. Compared with healthy-weight individuals, newly diagnosed patients who were overweight or obese had more severe weight loss during their disease onset and progression(P < 0.001). Individuals who were overweight or obese were associated with positive smoking history(P < 0.001). A significant difference in comorbidity of diabetes(P = 0.044) and coronary artery disease(P < 0.001) was identified between high b MI and normal-weight patients. After a median follow-up of 8 mo, the survival analysis showed no association between b MI and the overall survival(P = 0.90, n = 1783). When we stratified the whole cohort by pancreatic cancer stage, no statistically significant association between b MI and overall survival was found for resectable(P = 0.99, n = 217), unresectable locally advanced(P = 0.90, n = 316) and metastatic patients(P = 0.88, n = 1250), respectively. the results did not change when we used the b MI at diagnosis.CONCLUSION Our results showed no significance of b MI for the overall survival of PDAC patients. | Qing-long Jiang Cheng-feng Wang Yan-tao tian Huang Huang Shui-Sheng Zhang Dong-bing Zhao Jie Ma Wei Yuan Yue-Min Sun Xu Che Jian-Wei Zhang Yun-Mian Chu Ya-Wei Zhang Ying-tai Chen | 2017 | World Journal of Gastroenterology2017，23，34： | 7 |
| 6 | Gasdermin E-derived caspase-3 inhibitors effectively protect mice from acute hepatic failure显示文摘Programmed cell death(PCD),including apoptosis,apoptotic necrosis,and pyroptosis,is involved in various organ dysfunction syndromes.Recent studies have revealed that a substrate of caspase-3,gasdermin E(GSDME),functions as an effector for pyroptosis;however,few inhibitors have been reported to prevent pyroptosis mediated by GSDME.Here,we developed a class of GSDME-derived inhibitors containing the core structure of DMPD or DMLD.Ac-DMPD-CMK and Ac-DMLD-CMK could directly bind to the catalytic domains of caspase-3 and specifically inhibit caspase-3 activity,exhibiting a lower IC50 than that of Z-DEVD-FMK.Functionally,Ac-DMPD/DMLD-CMK substantially inhibited both GSDME and PARP cleavage by caspase-3,preventing apoptotic and pyroptotic events in hepatocytes and macrophages.Furthermore,in a mouse model of bile duct ligation that mimics intrahepatic cholestasis-related acute hepatic failure,Ac-DMPD/DMLD-CMK significantly alleviated liver injury.Together,this study not only identified two specific inhibitors of caspase-3 for investigating PCD but also,more importantly,shed light on novel lead compounds for treating liver failure and organ dysfunctions caused by PCD. | Wan-feng Xu Quan Zhang Chu-jie Ding Hui-yong Sun Yuan Che Hai Huang Yun Wang Jia-wei Wu Hai-ping Hao Li-juan Cao | 2021 | Acta Pharmacologica Sinica2021，42，1： | 7 |
| 7 | An individualized immune signature of pretreatment biopsies predicts pathological complete response to neoadjuvant chemoradiotherapy and outcomes in patients with esophageal squamous cell carcinoma显示文摘No clinically available biomarkers can predict pathological complete response(pCR)for esophageal squamous cell carcinomas(ESCCs)with neoadjuvant chemoradiotherapy(nCRT).Considering that antitumor immunity status is an important determinant for nCRT,we performed an integrative analysis of immune-related gene profiles from pretreatment biopsies and constructed the first individualized immune signature for pCR and outcome prediction of ESCCs through a multicenter analysis.During the discovery phase,14 differentially expressed immune-related genes(DEIGs)with greater than a twofold change between pCRs and less than pCRs( | Chaoqi Zhang Guochao Zhang Nan Sun Zhen Zhang Liyan Xue Zhihui Zhang Haijun Yang Yuejun Luo Xiaoli Zheng Yonglei Zhang Yufen Yuan Ruixue Lei Zhaoyang Yang Bo Zheng Le Wang Yun Che Feng Wang Sihui Wang Shugeng Gao Qi Xue Yi Zhang Jie He | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 7 |
| 8 | Phylogenetic Relationships of the Genus Paramesotriton(Caudata: Salamandridae) with the Description of a New Species from Qixiling Nature Reserve, Jiangxi, Southeastern China and a Key to the species显示文摘The matrilineal genealogy of the genus Paramesotriton is hypothesized based on DNA sequences from mitochondrial NADH subunit two(ND2) and its flanking tRNAs(tRNATrp and a partial tRNAAla). The genealogy identifies a highly divergent, unnamed lineage from Qixiling Nature Reserve, Jiangxi, China and places it as the sister taxon of P. chinensis. The newly discovered population differs from other congeners by several features of external morphology including having large clusters of dark brown conical warts on the dorsum of the head, lateral surface of the body and dorsolateral ridges. Its intermittent dorsal vertebral ridge is the same color as other parts of the dorsum and tail narrows gradually from the base to the tip. Further, the new population differs from all congeners by an uncorrected P-distance of more than 9.38% in ND2. Consequently, we describe the new species of Asian warty newt(Salamandridae) as Paramesotriton qixilingensis sp. nov. | Zhiyong YUAN Haipeng ZHAO Ke JIANG Mian HOU Lizhong HE Robert W.MURPHY Jing CHE | 2014 | Asian Herpetological Research2014，5，2： | 7 |
| 9 | Natatanuran frogs used the Indian Plate to step-stone disperse and radiate across the Indian Ocean显示文摘Investigating the evolutionary history of widespread higher taxa, subjected to multiple tectonic events, can provide evidence for or against various palaeogeographical models of early Earth history (1,2)Contemporary biotic distributions have been strongly influenced by events associated with the breakup of Gondwana into present-day Africa,Antarctica, Australia, South America,Madagascar and India, during the Late Mesozoic and Early Palaeogene (2)The fragmentation of Gondwana and subsequent tectonic drift ultimately allowed biotic exchanges between Laurasia and Gondwana [2,3], influencing the global distributions of many taxa. | Zhi-Yong Yuan Bao-Lin Zhang Christopher J.Raxworthy David W.Weisrock Paul M.Hime Jie-Qiong Jin Emily M.Lemmon Alan R.Lemmon Sean D.Holland Michelle L.Kortyna Wei-Wei Zhou Min-Sheng Peng Jing Che Elizabeth Prendini | 2019 | National Science Review2019，6，1： | 5 |
| 10 | Nutrient uptake requirements with increasing grain yield for rice in China显示文摘Improved estimates of nutrient requirements for rice(Oryza sativa L.)in China are essential to optimize fertilization regulation for increasing grain yields and reducing the potential of environmental negative influences,especially under high-yielding intensive systems.A database involving rice grain yields,nutrient concentrations and accumulations collected from on-field station experiments in the literatures published from 2000 to 2013 in China was developed to understand the relationships between grain yields and plant nutrient uptakes,and to quantify nutrient requirements for different yield levels.Considering all data sets,rice grain yield ranged from 1.4 to 15.2t ha^(-1)with the mean value of 7.84t ha^(-1),and ca.10.4%of yield observations were higher than the yield barrier level of 10 t ha^(-1).N requirement to produce one ton grain was 21.10 kg for the yield range<4.0t ha^(-1)with a high variation of 45.8%.Except of the yield range<4.0t ha^(-1),the values of N requirement,firstly increased from 18.78 kg for yield range 4.0-5.5 t ha^(-1)to 20.62 kg for yield range 7.0-8.5 t ha^(-1),then decreased slightly to 19.67 and19.17 kg for the yield range 8.5-10 and>10 t ha^(-1),respectively.Phosphorus(P)and potassium(K)requirements showed increasing trends,from 3.51 and 19.87 kg per t grain for<4.0 t ha^(-1)yield range to 4.10 and 21.70 kg for>10.0t ha^(-1)range.In conclusion,nutrient requirement varied with increasement of grain yield,and N,P and K presented various response trends,increasing,declining or stagnating,which would be of great benefit for improving fertilizer strategies. | CHE Sheng-guo ZHAO Bing-qiang LI Yan-ting YUAN Liang LIN Zhi-an HU Shu-wen SHEN Bing | 2016 | Journal of Integrative Agriculture2016，15，4： | 4 |
| 11 | Self-transforming ultrathin α-Co(OH)2 nanosheet arrays from metal-organic framework modified graphene oxide with sandwichlike structure for efficient electrocatalytic oxygen evolution显示文摘Developing efficient and low-cost electrocatalysts for oxygen evolution reaction(OER)with high electrochemical activity and durability for diverse renewable and sustainable energy technologies remains challenging.Herein,an ultrasonic-assisted and coordination modulation strategy is developed to construct sandwich-like metal-organic framework(MOF)derived hydroxide nanosheet(NS)arrays/graphene oxide(GO)composite via one-step self-transformation route.Inducing from unsteady state,the dodecahedral ZIF-67 with Co^2+in tetrahedral coordination auto-converts into defect-rich ultrathin layered hydroxides with the interlayered ion NO3-.The self-transforming a-Co(OH)2/GO nanosheet arrays from ZIF-67(Co(OH)2-GNS)change the coordination mode of Co^2+and bring about the exposure of more metal active sites,thereby enhancing the spatial utilization ratio within the framework.As monometal-based electrocatalyst,the optimized Co(OH)2-GNS exhibits remarkable OER catalytic performance evidenced by a low overpotential of 259 mV to achieve a current density of 10 mA·cm-2 in alkaline medium,even exceeding commercial RuO2.During the oxygen evolution process,electron migration can be accelerated by the interfacial/in-plane charge polarization and local electric field,corroborated by the off-axis electron holography.Density functional theory(DFT)calculations further studied the collaboration between ultrathin Co(OH)2 NS and GO,which leads to lower energy barriers of intermediate products and greatly promotes electrocatalytic property. | Mengqiu Huang Weiwei Liu Lei Wang Jiwei Liu Guanyu Chen Wenbin You Jie Zhang Lijun Yuan Xuefeng Zhang Renchao Che | 2020 | Nano Research2020，13，3： | 4 |
| 12 | HEIHE WATERSHED ALLIED TELEMETRY EXPERIMENTAL RESEARCH (HiWATER): Scientific Objectives and Experimental Design显示文摘 | Li Xin Cheng Guodong Liu Shaomin Xiao Qing Ma Mingguo Jin Rui Che Tao Liu Qinhuo Wang Weizhen Qi Yuan Wen Jianguang Li Hongyi Zhu Gaofeng Guo Jianwen Ran Youhua Wang Shuoguo Zhu Zhongli Zhou Jian Hu Xiaoli Xu Ziwei | 2013 | Bulletin of the American Meteorological Society2013，，8： | 3 |
| 13 | Synthesis and antitumor activity of novel 10-amino acids ester homocamptothecin analogues显示文摘九外消旋的 homocamptothecin 衍生物被综合,在 vitro antitumor,活动被标准 MTT 方法评估。结果比 iritecan 显示出复合有的更高的 antitumor 活动的那一些。 | Liang You Wan Nian Zhang Zhen Yuan Miao Wei Guo Xiao Ying Che Wen Ya Wang Chun Quan Sheng Jiang Zhong Yao Ting Zhou | 2008 | Chinese Chemical Letters2008，19，7： | 3 |
| 14 | Design and optimization of low-energy transfer orbit to Mars with multi-body environment显示文摘This paper discusses the problem of design and optimization of low-energy transfer orbit with multi-body environment. A new integrative method is proposed to effectively solve the problem, in which the parameterized patched manifolds in CR3BP(circular restricted three-body problems), the shape-based method with multi-body environment, the homotopic method with multi-body environment, and the low-thrust capturing and descending algorithm with multi-body environment are all included. Firstly, the parameters describing the patched manifolds in CR3 BP are optimized until the least total absolute velocity increment has been got, including the employment of the shape-based method with multi-body environment. Secondly, the low-thrust control laws of the transfer orbit are optimized employing the homotopic method with multi-body environment that transfers the fuel optimization problem to an easier energy optimization problem. Thirdly, the low-thrust descending orbit around Mars is computed using the laws proposed in this paper. As a typical example, the Earth-Mars transfer orbit design is discussed. The results showed that the parameters describing the patched manifolds could be optimized by the DE(differential evolution) algorithm effectively; the homotopic method with multi-body environment could get the optimal value that meets the first order optimality conditions; and the low-thrust descending orbit could effectively be captured by Mars and finally become a circular parking orbit around it by the hypothesis control laws proposed in this paper. It shows that the final fuel cost is much less than the optimal transfer in the patched two-body problems. In conclusion, the method proposed in this paper could effectively solve the low-energy low-thrust optimal control problem in multi-body environment for the future deep space explorations. | LU Yi LI HengNian LI JiSheng CHE Zheng YANG YiKang YANG Yuan SUN Yang | 2015 | Science China(Technological Sciences)2015，58，10： | 3 |
| 15 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 16 | The mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus显示文摘A mutation network for the hemagglutinin gene(HA) of the novel type A(H1N1) influenza virus was constructed.Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from Mexico was likely the original type in this epidemic.Based on the 658A and 1408T mutations in HA,the viruses evolving into this epidemic were divided into three categories,the Mexico,the transitional and the New York type.The three groups of viruses presented distinctive clustering features in their geographic distributions. | HE YunGang DING GuoHui BIAN Chao HUANG Zhong LAN Ke SUN Bing WANG XueCai LI YiXue WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang JIN WeiRong XIONG Hui DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenAn ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN Li | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 17 | Gender associates with both susceptibility to infection and pathogenesis of SARS-CoV-2 in Syrian hamster显示文摘Epidemiological studies of the COVID-19 patients have suggested the male bias in outcomes of lung illness.To experimentally demonstrate the epidemiological results,we performed animal studies to infect male and female Syrian hamsters with SARS-CoV-2.Remarkably,high viral titer in nasal washings was detectable in male hamsters who presented symptoms of weight loss,weakness,piloerection,hunched back and abdominal respiration,as well as severe pneumonia,pulmonary edema,consolidation,and fibrosis.In contrast with the males,the female hamsters showed much lower shedding viral titers,moderate symptoms,and relatively mild lung pathogenesis.The obvious differences in the susceptibility to SARS-CoV-2 and severity of lung pathogenesis between male and female hamsters provided experimental evidence that SARS-CoV-2 infection and the severity of COVID-19 are associated with gender. | Lunzhi Yuan Huachen Zhu Ming Zhou Jian Ma Rirong Chen Yao Chen Liqiang Chen Kun Wu Minping Cai Junping Hong Lifeng Li Che Liu Huan Yu Yali Zhang Jia Wang Tianying Zhang Shengxiang Ge Jun Zhang Quan Yuan Yixin Chen Qiyi Tang Honglin Chen Tong Cheng Yi Guan Ningshao Xia | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 3 |
| 18 | A new species of rain-pool frog (Dicroglossidae: Fejervarya) from western Thailand显示文摘We describe a new species, Fejervarya muangkanensis sp. nov., based on a series of specimens collected from Ban Tha Khanun, Thong Pha Phum District, Kanchanaburi Province, Thailand. The new species is easily distinguished from its congeners by morphological and molecular data, and can be diagnosed by the following characters:(1) small size(adult male snout-vent length(SVL) 33.5 mm; female SVL 40.0–40.9 mm);(2) tympanum small, discernible but unclear;(3) poorly developed toe webbing;(4) no lateral line system in adults;(5) characteristic 'Fejervaryan' lines present in females; and(6) femoral glands absent. Molecular phylogenetic analysis of mitochondrial 16 S r RNA further supports it as a distinct lineage and distinguishes it from its congeners for which sequences are available. | Chatmongkon Suwannapoom Zhi-Yong Yuan Ke Jiang Fang Yan Wei Gao Jing Che | 2017 | Zoological Research2017，38，5： | 3 |
| 19 | Glucose oxidase as a blocking agent-based signal amplification strategy for the fabrication of label-free amperometric immunosensors显示文摘An effective electrochemical signal amplification strategy based on enzyme membrane modification and redox probe immobilization was proposed to construct an amperometric immunosensor.L-cysteine@ferrocene functionalized chitosan,which possessed not only efficient redox-activity but also excellent film-forming ability,was coated on the bare glass carbon electrode. Moreover,the thiol groups(SH)in the ferrocenyl compound were used for gold nanoparticles immobilization via the strong bonding interaction,which could further be utilized for the immobilization of antibody biomolecules with well-retained bioactivities.Finally,glucose oxidase(GOD)as the enzyme membrane was employed to block the possible remaining active sites and avoid the nonspecific adsorption.With the excellent electrocatalytic properties of GOD towards glucose,the amplification of antigen-antibody interaction and the enhanced sensitivity could be achieved.Under the optimal conditions,the linear range of the proposed immunosensor for the determination of carcinoembryonic antigen(CEA)was from 0.05 to 100 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).Moreover,the immunosensor exhibited good selectivity,stability and reproducibility, which provided a promising potential for clinical immunoassay. | SONG ZhongJu YUAN Ruo CHAI YaQin CHE Xin LV Ping | 2011 | Science China Chemistry2011，54，3： | 3 |
| 20 | A biomimetic and bioactive scaffold with intelligently pulsatile teriparatide delivery for local and systemic osteoporosis regeneration显示文摘Osteoporosis is one of the most disabling consequences of aging,osteoporotic fractures and higher risk of the subsequent fractures leading to substantial disability and deaths,indicating both local fractures healing and the early anti-osteoporosis therapy are of great significance.Teriparatide is strong bone formation promoter effective in treating osteoporosis,while side effects limit clinical applications.Traditional drug delivery is lack of sensitive and short-term release,finding a new non-invasive and easily controllable drug delivery to not only repair the local fractures but also improve total bone mass has remained a great challenge.Thus,bioinspired by the natural bone components,we develop appropriate interactions between inorganic biological scaffolds and organic drug molecules,achieving both loaded with the teriparatide in the scaffold and capable of releasing on demand.Herein,biomimetic bone microstructure of mesoporous bioglass,a near-infrared ray triggered switch,thermosensitive liposomes based on a valve,and polydopamine coated as a heater is developed rationally for osteoporotic bone regeneration.Teriparatide is pulsatile released from intelligent delivery,not only rejuvenating osteoporotic bone defect,but also presenting strong systemic anti-osteoporosis therapy.This biomimetic bone carrying novel drug delivery platform is well worth expecting to be a new promising strategy and clinically commercialized to help patients survive from the osteoporotic fracture. | Lingbin Che Ying Wang Dongyong Sha Guangyi Li Ziheng Wei Changsheng Liu Yuan Yuan Dianwen Song | 2023 | Bioactive Materials2023，，1： | 3 |
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