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| 1 | Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression显示文摘Recently,increasing evidence has suggested the association between gut dysbiosis and Alzheimer's disease(AD)progression,yet the role of gut microbiota in AD pathogenesis remains obscure.Herein,we provide a potential mechanistic link between gut microbiota dysbiosis and neuroinflammation in AD progression.Using AD mouse models,we discovered that,during AD progression,the alteration of gut microbiota composition leads to the peripheral accumulation of phenylalanine and isoleucine,which stimulates the differentiation and proliferation of pro-inflammatory T helper 1(Thl)cells.The brain-infiltrated peripheral Th1 immune cells are associated with the Ml microglia aaivation,contributing to AD-associated neuroinflammation.Importantly,the elevation of phenylalanine and isoleucine concentrations and the increase of Th1 cell frequency in the blood were also observed in two small independent cohorts of patients with mild cognitive impairment(MCI)due to AD.Furthermore,GV-971,a sodium oligomannate that has demonstrated solid and consistent cognition improvement in a phase 3 clinical trial in China,suppresses gut dysbiosis and the associated phenylalanine/isoleucine accumulation,harnesses neuroinflammation and reverses the cognition impairment.Together,our findings highlight the role of gut dysbiosis-promoted neuroinflammation in AD progression and suggest a novel strategy for AD therapy by remodelling the gut microbiota. | Xinyi Wang Guangqiang Sun Teng Feng Jing Zhang Xun Huang Tao Wang Zuoquan Xie Xingkun Chu Jun Yang Huan Wang Shuaishuai Chang Yanxue Gong Lingfei Ruan Guanqun Zhang Siyuan Yan Wen Lian Chen Du Dabing Yang Qingli Zhang Feifei Lin Jia Liu Haiyan Zhang Changrong Ge Shifu Xiao Jian Ding Meiyu Geng | 2019 | Cell Research2019，29，10： | 192 |
| 2 | Terrestrial carbon sinks in China and around the world and their contribution to carbon neutrality显示文摘Enhancing the terrestrial ecosystem carbon sink(referred to as terrestrial C sink) is an important way to slow down the continuous increase in atmospheric carbon dioxide(CO_(2)) concentration and to achieve carbon neutrality target.To better understand the characteristics of terrestrial C sinks and their contribution to carbon neutrality,this review summarizes major progress in terrestrial C budget researches during the past decades,clarifies spatial patterns and drivers of terrestrial C sources and sinks in China and around the world,and examines the role of terrestrial C sinks in achieving carbon neutrality target.According to recent studies,the global terrestrial C sink has been increasing from a source of (-0.2±0.9) Pg C yr^(-1)(1 Pg=1015g)in the 1960s to a sink of (1.9±1.1) Pg C yr^(-1) in the 2010s.By synthesizing the published data,we estimate terrestrial C sink of 0.20–0.25 Pg C yr^(-1) in China during the past decades,and predict it to be 0.15–0.52 Pg C yr^(-1) by 2060.The terrestrial C sinks are mainly located in the mid-and high latitudes of the Northern Hemisphere,while tropical regions act as a weak C sink or source.The C balance differs much among ecosystem types:forest is the major C sink;shrubland,wetland and farmland soil act as C sinks;and whether the grassland functions as C sink or source remains unclear.Desert might be a C sink,but the magnitude and the associated mechanisms are still controversial.Elevated atmospheric CO_(2) concentration,nitrogen deposition,climate change,and land cover change are the main drivers of terrestrial C sinks,while other factors such as fires and aerosols would also affect ecosystem C balance.The driving factors of terrestrial C sink differ among regions.Elevated CO_(2) concentration and climate change are major drivers of the C sinks in North America and Europe,while afforestation and ecological restoration are additionally important forcing factors of terrestrial C sinks in China.For future studies,we recommend the necessity for intensive and long-term ecosystem C monitoring over broad geographic scale to improve terrestrial biosphere models for accurately evaluating terrestrial C budget and its dynamics under various climate change and policy scenarios. | Yuanhe Yang Yue Shi Wenjuan Sun Jinfeng Chang Jianxiao Zhu Leiyi Chen Xin Wang Yanpei Guo Hongtu Zhang Lingfei Yu Shuqing Zhao Kang Xu Jiangling Zhu Haihua Shen Yuanyuan Wang Yunfeng Peng Xia Zhao Xiangping Wang Huifeng Hu Shiping Chen Mei Huang Xuefa Wen Shaopeng Wang Biao Zhu Shuli Niu Zhiyao Tang Lingli Liu Jingyun Fang | 2022 | Science China(Life Sciences)2022，65，5： | 32 |
| 3 | Toxicity of graphene oxide and multi-walled carbon nanotubes against human cells and zebrafish显示文摘Graphene possesses unique physical and chemical properties, which have inspired a wide range of potential biomedical applications. However, little is known about the adverse effects of graphene on the human body and ecological environment. The purpose of our work is to make assessment on the toxicity of graphene oxide (GO) against human cell line (human bone marrow neuroblastoma cell line and human epithelial carcinoma cell line) and zebrafish (Danio rerio) by comparing the toxic effects of GO with its sister, multi-walled carbon nanotubes (MWNTs). The results show that GO has a moderate toxicity to organisms since it can induce minor (about 20%) cell growth inhibition and slight hatching delay of zebrafish embryos at a dosage of 50 mg/L, but did not result in significant increase of apoptosis in embryo, while MWNTs exhibit acute toxicity leading to a strong inhibition of cell proliferation and serious morphological defects in developing embryos even at relatively low concentration of 25 mg/L. The distinctive toxicity of GO and MWNTs should be ascribed to the different models of interaction between nanomaterials and organisms, which arises from the different geometric structures of nanomaterials. Collectively, our work suggests that GO does actual toxicity to organisms posing potential environmental risks and the result is also shedding light on the geometrical structure-dependent toxicity of graphitic nanomaterials. | CHEN LiQiang HU PingPing ZHANG Li HUANG SiZhou LUO LingFei HUANG ChengZhi | 2012 | Science China Chemistry2012，55，10： | 12 |
| 4 | Recent progress in NIR-Ⅱ emitting lanthanide-based nanoparticles and their biological applications显示文摘The second near-infrared(NIR-Ⅱ,1000-1700 nm)window provides a superior optical platform with high resolution,deep penetration and high signal-to-noise ratios(SNRs),which results from the intrinsic low scattering and auto fluorescence in biological tissues.As one of the promising NIR-Ⅱemitting probes,lanthanide based nanoparticles(LnNPs)exhibit high photo stability and chemostability,long photoluminescence lifetimes,low long-term cytotoxicity and narrow emission bandwidths.All these merits have spurred the evolution of related bio-optics and a variety of biomedical applications of LnNPs.This mini-review discusses the most recent advances in both the design-the composition and surface modifications-and the applications of NIR-Ⅱemitting LnNPs in bioimaging,disease diagnosis and therapy.We also summarize the current limits and challenges facing the applications of LnNPs as well as discuss the directions of future development. | Suwan Ding Lingfei Lu Yong Fan Fan Zhang | 2020 | Journal of Rare Earths2020，38，5： | 9 |
| 5 | Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction显示文摘 | Xiao-E Jia Ke Ma Tao Xu Lei Gao Shuang Wu Cong Fu Wenjuan Zhang Zhizhang Wang Kaiyu Liu Mei Dong Changbin Jing Chunguang Ren Zhiwei Dong Yi Chen Yi Jin Qiuhua Huang Xing Chang Min Deng Li Li Lingfei Luo Jun Zhu Yongjun Dang Hung-Chun Chang Leonard I Zon Yi Zhou Saijuan Chen Weijun Pan | 2015 | Cell Research2015，25，8： | 8 |
| 6 | Variable structure control with sliding mode prediction for discrete-time nonlinear systems显示文摘新可变结构基于滑动模式预言控制算法因为分离时间的非线性的系统的一个类被介绍。由雇用一个特殊模特儿预言滑动模式价值的未来,;联合反馈修正;是的后退的地平线优化方法广泛地在预兆的控制策略上适用,一条分离时间的可变结构控制法律被构造。靠近环的系统被证明与未特别指出的边界有坚韧性到不确定性。数字模拟;摆钟实验结果说明靠近环的系统拥有需要的性能例如强壮的坚韧性,快集中;啁啾的消除。 | Lingfei XIAO Hongye SU Xiaoyu ZHANG Jian CHU | 2006 | 控制理论与应用(英文版)2006，4，2： | 4 |
| 7 | Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis显示文摘The function of serrate(SE)in miRNA biogenesis in Arabidopsis is well elucidated,whereas its role in plant drought resistance is largely unknown.In this study,we report that MdSE acts as a negative regulator of apple(Malus×domestica)drought resistance by regulating the expression levels of MdMYB88 and MdMYB124 and miRNAs,including mdm-miR156,mdm-miR166,mdm-miR172,mdm-miR319,and mdm-miR399.MdSE interacts with MdMYB88 and MdMYB124,two positive regulators of apple drought resistance.MdSE decreases the transcript and protein levels of MdMYB88 and MdMYB124,which directly regulate the expression of MdNCED3,a key enzyme in abscisic acid(ABA)biosynthesis.Furthermore,MdSE is enriched in the same region of the MdNECD3 promoter where MdMYB88/MdMYB124 binds.Consistently,MdSE RNAi transgenic plants are more sensitive to ABA-induced stomatal closure,whereas MdSE OE plants are less sensitive.In addition,under drought stress,MdSE is responsible for the biogenesis of mdm-miR399,a negative regulator of drought resistance,and negatively regulates miRNAs,including mdm-miR156,mdm-miR166,mdm-miR172,and mdm-miR319,which are positive regulators of drought resistance.Taken together,by revealing the negative role of MdSE,our results broaden our understanding of the apple drought response and provide a candidate gene for apple drought improvement through molecular breeding. | Xuewei Li Pengxiang Chen Yinpeng Xie Yan Yan Liping Wang Huan Dang Jing Zhang Lingfei Xu Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 4 |
| 8 | The role of China's terrestrial carbon sequestration 2010-2060 in offsetting energy-related CO_(2) emissions显示文摘Energy consumption dominates annual CO_(2) emissions in China.It is essential to significantly reduce CO_(2) emissions from energy consumption to reach national carbon neutrality by 2060,while the role of terrestrial carbon sequestration in offsetting energy-related CO_(2) emissions cannot be underestimated.Natural climate solutions(NCS),including improvements in terrestrial carbon sequestration,represent readily deployable options to offset anthropogenic greenhouse gas emissions.However,the extent to which China’s terrestrial carbon sequestration in the future,especially when target-oriented managements(TOMs)are implemented,can help to mitigate energy-related CO_(2) emissions is far from certain.By synthesizing available findings and using several parameter-sparse empirical models that have been calibrated and/or fitted against contemporary measurements,we assessed China’s terrestrial carbon sequestration over 2010-2060 and its contribution to offsetting national energy-related CO_(2) emissions.We show that terrestrial C sequestration in China will increase from 0.375±0.056(mean±standard deviation)Pg C yr^(-1) in the 2010s to 0.458±0.100 Pg C yr^(-1)under RCP2.6 and 0.493±0.108 Pg C yr^(-1) under the RCP4.5 scenario in the 2050s,when TOMs are implemented.The majority of carbon sequestration comes from forest,accounting for 67.8-71.4%of the total amount.China’s terrestrial ecosystems can offset 12.2-15.0%and 13.4-17.8%of energy-related peak CO_(2) emissions in 2030 and 2060,respectively.The implementation of TOMs contributes 11.9%of the overall terrestrial carbon sequestration in the 2020s and 23.7%in the 2050s.The most likely strategy to maximize future NCS effectiveness is a full implementation of all applicable cost-effective NCS pathways in China.Our findings highlight the role of terrestrial carbon sequestration in offsetting energy-related CO_(2) emissions and put forward future needs in the context of carbon neutrality. | Yao Huang Wenjuan Sun Zhangcai Qin Wen Zhang Yongqiang Yu Tingting Li Qing Zhang Guocheng Wang Lingfei Yu Yijie Wang Fan Ding Ping Zhang | 2022 | National Science Review2022，9，8： | 4 |
| 9 | CPPU may induce gibberellin-independent parthenocarpy associated with PbRR9 in‘Dangshansu’pear显示文摘Parthenocarpy is a valuable trait in self-incompatible plants,such as pear.N-(2-chloro-4-pyridyl)-N’-phenylurea(CPPU),a synthetic cytokinin analog,can induce parthenocarpy in pear(Pyrus spp.),but the mechanism of induction is unclear.To investigate the role of gibberellin in CPPU-induced parthenocarpy in pear,CPPU supplemented with paclobutrazol(PAC)was sprayed onto‘Dangshansu’pear.We found that the fruit set rate of pear treated with CPPU supplemented with PAC was identical to that in a CPPU-alone treatment group.In regard to cell development,CPPU mainly promoted hypanthium cell division and expansion,and PAC application had no influence on CPPU-induced cell development.RNA sequencing revealed that gibberellin 20 oxidase and gibberellin 3 oxidase genes were not differentially expressed following CPPU treatment.According to the analysis of fruit phytohormone content,the CPPU treatments did not induce gibberellin biosynthesis.These results suggest that CPPU-induced parthenocarpy may be gibberellin independent in‘Dangshansu’pear.After CPPU treatment,the indole acetic acid(IAA)content in fruit was significantly increased,and the abscisic acid(ABA)content was significantly decreased.Similarly,RNA sequencing revealed that many genes involved in the auxin and ABA pathways were significantly differentially expressed in the CPPU treatment groups;among them,indole-3-pyruvate monooxygenase(YUCCA)was significantly upregulated and 9-cis-epoxycarotenoid dioxygenase(NCED)was significantly downregulated.IAA and ABA may thus play important roles in CPPU-induced parthenocarpy.PbTwo-component response regulator9(PbRR9),PbYUCCA4,and PbNCED6 were then selected to further elucidate the mechanism of CPPU-induced parthenocarpy.A yeast one-hybrid assay indicated that PbRR9 can combine with the PbYUCCA4 and PbNCED6 promoters.Dual luciferase assays revealed that PbRR9 can promote and repress the activities of the PbYUCCA4 and PbNCED6 promoters,respectively.After the transient expression of PbRR9 in fruits,PbYUCCA4 expression was significantly upregulated,and PbNCED6 expression was significantly downregulated.This study uncovered a CPPU-induced parthenocarpy mechanism that is different from that in tomato.CPPU may upregulate PbYUCCA4 and downregulate PbNCED6 by upregulating PbRR9,thereby increasing IAA content and decreasing ABA content to ultimately induce parthenocarpy in‘Dangshansu’pear.However,because only a single time point was used and because‘botanical’and‘accessory’fruits have different structures,this conclusion is still preliminary. | Liu Cong Ting Wu Hanting Liu Huibin Wang Haiqi Zhang Guangping Zhao Yao Wen Qianrong Shi Lingfei Xu Zhigang Wang | 2020 | Horticulture Research2020，7，1： | 4 |
| 10 | Mobile Content Distribution with Vehicular Cloud in Urban VANETs显示文摘Plenty of multimedia contents such as traffic images, surveillance video, music and movie will flood into vehicular ad hoc networks. However, content distribution over VANETs is not a easy task, due to the high mobility of vehicles and intermittent connectivity. Infrastructure-based scheme can relieve the problem, but with a large amount of investment. In this paper, we propose a mobile content distribution scheme based on roadside parking cloud(RPC), which is formed by the parked car on the roadside, and mobile cloud(MC), which is formed by moving cars on the road. According to a trip history model, a mobile car can estimate its following trajectory. When it wants to download the content, gateway node of the RPC will work out a downloading schedule, which tells it how much chunks it can download from which RPCs. Moreover, the helper of the mobile car in mobile cloud would deliver specified chunks to it when there is lack of RPC in the following trip. Simulation results show that cloud-based scheme performs better than inter-vehicle communication approach and cluster-based scheme. | Haigang Gong Lingfei Yu Nianbo Liu Xue Zhang | 2016 | China Communications2016，13，8： | 3 |
| 11 | A Survey on Negative Transfer显示文摘Transfer learning(TL)utilizes data or knowledge from one or more source domains to facilitate learning in a target domain.It is particularly useful when the target domain has very few or no labeled data,due to annotation expense,privacy concerns,etc.Unfortunately,the effectiveness of TL is not always guaranteed.Negative transfer(NT),i.e.,leveraging source domain data/knowledge undesirably reduces learning performance in the target domain,and has been a long-standing and challenging problem in TL.Various approaches have been proposed in the literature to address this issue.However,there does not exist a systematic survey.This paper fills this gap,by first introducing the definition of NT and its causes,and reviewing over fifty representative approaches for overcoming NT,which fall into three categories:domain similarity estimation,safe transfer,and NT mitigation.Many areas,including computer vision,bioinformatics,natural language processing,recommender systems,and robotics,that use NT mitigation strategies to facilitate positive transfers,are also reviewed.Finally,we give guidelines on NT task construction and baseline algorithms,benchmark existing TL and NT mitigation approaches on three NT-specific datasets,and point out challenges and future research directions.To ensure reproducibility,our code is publicized at http://gffzz188fe103f8f1460as5kcwv5kqupq560b0.ffgz.tsg.suse.edu.cn/chamwen/NT-Benchmark. | Wen Zhang Lingfei Deng Lei Zhang Dongrui Wu | 2023 | IEEE/CAA Journal of Automatica Sinica2023，10，2： | 3 |
| 12 | Assessment of the risk of infectious aerosols leaking to the environment from BSL-3 laboratory HEPA air filtration systems using model bacterial aerosols显示文摘To assess the risk of infectious bacterial aerosols leaking to the environment, the filtration efficiency of a biosafety level 3(BSL-3) laboratory high-efficiency particulate(HEPA) filter was investigated using the aerosolized bacteria Serratia marcescens. The aerosol size was measured using an Andersen sampler. Eight first stage HEPA filters(numbered 1-8) were distributed in contaminated labs and exhausts from each0f the first stage HEPA filters were aggregated and filtered through one second stage HEPA filter before being released to the environment. In total, 8 first-stage and 1 second-stage HEPA filters from the BSL-3 air purification system were analyzed. No S. marcescens was detected in first stage filters 1,2,4,5,7 and 8 and the second stage HEPA filter. The filtration efficiencies against aerosolized S. marcescens were >99.9999%.First stage filter numbers 3 and 6 had filtration efficiencies of 99.9825% and 99.9906%, respectively. When filter number 3 was replaced by a new filter and the bracket for filter number 6 was sealed, no aerosolized S. marcescens was detected in the filtered air. Our work suggests that the BSL-3 laboratory HEPA filter air purification system is effective against bacterial aerosols, with little to no bacterial leakage into the environment. | Zhanbo Wen Wenhui Yang Na Li Jie Wang Lingfei Hu Jinsong Li Zhe Yin Ke Zhang Xiaokai Dong | 2014 | Particuology2014，12，2： | 2 |
| 13 | Asymmetric interfaces and high-TC ferromagnetic phase in La_(0.67)Ca_(0.33)MnO_(3)/SrRuO_(3)superlattices显示文摘Interfacial magnetism in functional oxide heterostructures not only exhibits intriguing physical phenomena but also implies great potential for device applications.In these systems,interfacial structural and electronic reconstructions are essential for improving the stability and tunability of the magnetic properties.In this work,we constructed ultra-thinLa_(0.67)Ca_(0.33)MnO_(3)/SrRuO_(3)(SRO)layers into superlattices,which exhibited a robust ferromagnetic phase.The high Curie temperature(TC)reaches 291 K,more than 30 K higher than that of bulk LCMO.We found that the LCMO/SRO superlattices consisted of atomically-sharp and asymmetric heterointerfaces.Such a unique interface structure can trigger a sizable charge transfer as well as a ferroelectric-like polar distortion.These two interfacial effects cooperatively stabilized the high-T_(C)ferromagnetic phase.Our results could pave a promising approach towards effective control of interfacial magnetism and new designs of oxide-based spintronic devices. | Lili Qu Da Lan Liang Si Chao Ma Shasha Wang Liqiang Xu Kexuan Zhang Feng Jin Zixun Zhang Enda Hua Binbin Chen Guanyin Gao Feng Chen Haifeng Du Karsten Held Lingfei Wang Wenbin Wu | 2021 | Nano Research2021，14，10： | 1 |
| 14 | Frequency tracking and locking method used in resonator micro-optic gyro 显示文摘 | Hong Lingfei Zhang Chunxi Feng Lishuang | 2012 | Sensor Letters2012，10，12： | 1 |
| 15 | A compact frequency-reconfigurable patch antenna for Beidou(COMPASS)navigation system显示文摘 | Sun Chao Zheng Huili Zhang Lingfei | 2014 | IEEE Antennas and Wireless Propagation Letters2014，13，5： | 1 |
| 16 | NSE, a potential bi- omarker, is closely connected to diabetic peripheral neuropathy 显示文摘 | Jianbo Li Lingfei Yan Hongman Zhang | 2013 | Diabetes Care2013，39，： | 1 |
| 17 | Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction显示文摘Background:Our previous work suggested that microtubule associated protein 4(MAP4)phosphorylation led to mitochondrial dysfunction in MAP4 phosphorylation mutant mice with cardiomyopathy,but the detailed mechanism was still unknown.Thus,the aim of this study was to investigate the potential mechanism involved in mitochondrial dysfunction responsible for cardiomyopathy.Methods:The present study was conducted to explore the potential mechanism underlying the mitochondrial dysfunction driven by MAP4 phosphorylation.Strain of mouse that mimicked constant MAP4 phosphorylation(S737 and S760)was generated.The isobaric tag for relative and absolute quantitation(iTRAQ)analysis was applied to the heart tissue.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG),and protein-protein interaction(PPI)were all analyzed on the basis of differential expressed proteins(DEPs).Results:Among the 72 cardiac DEPs detected between the two genotypes of mice,12 were upregulated and 60 were downregulated.GO analysis showed the biological process,molecular function,and cellular component of DEPs,and KEGG enrichment analysis linked DEPs to 96 different biochemical pathways.In addition,the PPI network was also extended on the basis of DEPs as the seed proteins.Three proteins,including mitochondrial ubiquitin ligase activator of NF-κB 1,reduced form of nicotinamide adenine dinucleotide(NADH)-ubiquinone oxidoreductase 75 kDa subunit,mitochondrial and growth arrest,and DNA-damage-inducible proteins-interacting protein 1,which play an important role in the regulation of mitochondrial function,may correlate with MAP4 phosphorylationinduced mitochondrial dysfunction.Western blot was used to validate the expression of the three proteins,which was consistent with iTRAQ experiments.Conclusions:These findings revealed that the DEPs caused by MAP4 phosphorylation in heart tissue using iTRAQ technique and may provide clues to uncover the potential mechanism of MAP4 phosphorylation-induced mitochondrial dysfunction. | Lingfei Li Junhui Zhang Qiong Zhang Yuesheng Huang Jiongyu Hu | 2019 | Burns & Trauma2019，7，1： | 1 |
| 18 | Broadband UHF RFID tag antenna with a pair of U slots mountable on metallic objects 显示文摘 | Mo Lingfei Zhang Hongjian Zhou Hongliang | 2008 | Electronics Letters2008，44，20： | 1 |
| 19 | Analysis of dipole-like UHF RFID tags close to metallic surfaces 显示文摘 | Mo Lingfei Zhang Hongjian Zhou Hongliang | 2009 | Journal of Zhejiang University--Science A2009，10，8： | 1 |
| 20 | Energy-efficient Traffic Grooming Under Sliding Scheduled Traffic Model for IP over WDM Optical Networks显示文摘In this paper,we investigate on the problem of energy-efficient traffic grooming under sliding scheduled traffic model for IP over WDM optical networks,so as to minimize the total energy consumption of the core network.We present a two-layer auxiliary graph model and propose a new energyefficient traffic grooming heuristic named Two-Dimension Green Traffic Grooming(TDGTG) algorithm,which takes both space and time factors into consideration for network energy efficiency.We compare our proposed TDGTG algorithm with the previous traffic grooming algorithms for scheduled traffic model in terms of total energy consumption and blocking probability.The simulation results in three typical carrier topologies show the efficiency of our proposed TDGTD algorithm. | ZHANG Xiaoning XU Lingfei | 2014 | China Communications2014，11，7： | 1 |
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