|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 2 | p38 MAPK inhibition alleviates experimental acute pancreatitis in mice显示文摘BACKGROUND: The mitogen-activated protein kinases (MAPKs) signaling pathway is involved in inflammatory process. However,the mechanism is not clear. The present study was to investigate the role of p38 MAPK in acute pancreatitis in mice.METHODS: Mice were divided into 4 groups: saline control; acute pancreatitis induced with repeated injections of cerulein; control plus p38 MAPK inhibitor SB203580; and acute pancreatitis plus SB203580. The pancreatic histology, pancreatic enzymes, cytokines, myeloperoxidase activity, p38 MAPK and heat shock protein (HSP) 60 and 70 were evaluated.RESULTS: Repeated injections of cerulein resulted in acute pancreatitis in mice, accompanying with the activation of p38 MAPK and overexpression of HSP60 and HSP70 in the pancreatic tissues. Treatment with SB203580 significantly inhibited the activation of p38 MAPK, and furthermore, inhibited the expression of HSP60 and HSP70 in the pancreas, the inflammatory cytokines in the serum, and myeloperoxidase activity in the lung.CONCLUSION: The p38 MAPK signaling pathway is involved in the regulation of inflammatory response and the expression of HSP60 and HSP70 in acute pancreatitis. | Ming-Hua Cao Jing Xu Hai-Dong Cai Zhong-Wei Lv Ya-Jing Feng Kun Li Chun-Qiu Chen Yong-Yu Li | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，1： | 15 |
| 3 | Acupuncture at Baihui and Dazhui reduces brain cell apoptosis in heroin readdicts显示文摘Acupuncture at Baihui(GV20) and Dazhui(GV14) reduces neuronal loss and attenuates ultrastructural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction through changes to brain cell ultrastructure remains unknown. In this study, cell apoptosis was observed in the hippocampus and frontal lobe of heroin readdicted rats by electron microscopy. Immunohistochemical staining displayed a reduction in Bcl-2 expression and an increase in Bax expression in the hippocampus and frontal lobe. After rats were given acupuncture at Baihui and Dazhui, the pathological damage in the hippocampus and frontal lobe was significantly reduced, Bcl-2 expression was upregulated and Bax expression was downregulated. Acupuncture exerted a similar effect with methadone, a commonly used drug for clinical treatment of drug addiction. Experimental findings suggest that acupuncture at Dazhui and Baihui can prevent brain cell apoptosis in heroin readdicted rats. | Xiaorong Hou Rongjun Zhang Hang Lv Xinghui Cai Guangchuan Xie Xiaoge Song | 2014 | Neural Regeneration Research2014，9，2： | 12 |
| 4 | Preparation of a Crosslinked Chitosan Coated Calcium Sulfate Whisker and Its Reinforcement in Polyvinyl Chloride显示文摘A calcium sulfate whisker(CSW) coated with glutaraldehyde crosslinked chitosan(GACS) was prepared to reinforce polyvinyl chloride(PVC) in this study. The results show that the optimum concentration of both chitosan(CS) and glutaraldehyde(GA) is 0.05 wt%. The tensile strength, impact strength, flexural modulus and vicat softening temperature of the PVC composite with 12 wt% of modified CSW are increased by 17.5%, 40.4%, 0.8% and 3.8% compared with those of the PVC composite with 12 wt% of unmodified CSW, and by 2.9%, 42.4%, 27.1% and 6.8% compared with those of pure PVC, respectively. The dynamic mechanical analysis results indicate that the modified CSW/PVC composite exhibits much higher storage modulus and glass transition temperature than those of unmodified CSW/PVC composite and pure PVC.In addition, the modified CSW/PVC composite also demonstrates good thermal properties with a high rapidest decomposition temperature(Trpd) and char residue. The scanning electron microscopy images of tensile-fractured surfaces show that the modified CSW has a strong interfacial adhesion with PVC matrix. | Jiayang Cui Yangben Cai Wenjin Yuan Zhongfei Lv Shiai Xu | 2016 | Journal of Materials Science & Technology2016，32，8： | 12 |
| 5 | Treatment of coking wastewater by a novel electric assisted micro-electrolysis filter显示文摘A newly designed electric assisted micro-electrolysis filter(E-ME) was developed to investigate its degradation efficiency for coking wastewater and correlated characteristics. The performance of the E-ME system was compared with separate electrolysis(SE) and micro-electrolysis(ME) systems. The results showed a prominent synergistic effect on COD removal in E-ME systems. Gas chromatography/mass spectrometry(GC–MS) analysis confirmed that the applied electric field enhanced the degradation of phenolic compounds.Meanwhile, more biodegradable oxygen-bearing compounds were detected. SEM images of granular activated carbon(GAC) showed that inactivation and blocking were inhibited during the E-ME process. The effects of applied voltage and initial p H in E-ME systems were also studied. The best voltage value was 1 V, but synergistic effects existed even with lower applied voltage. E-ME systems exhibited some p H buffering capacity and attained the best efficiency in neutral media, which means that there is no need to adjust p H prior to or during the treatment process. Therefore, E-ME systems were confirmed as a promising technology for treatment of coking wastewater and other refractory wastewater. | Ruosong Xie Miaomiao Wu Guangfei Qu Ping Ning Yingying Cai Pei Lv | 2018 | Journal of Environmental Sciences2018，30，4： | 12 |
| 6 | Mechanism of aqueous fructus aurantii immaturus extracts in neuroplexus of cathartic colons显示文摘AIM: To examine the effect of aqueous fructus aurantii immaturus(FAI) extracts on the intestinal plexus of cathartic colons.METHODS: Cathartic colons were induced in rats with dahuang,a laxative used in traditional Chinese medicine. Once the model was established(after approximately 12 wk),rats were administered mosapride(1.54 mg/kg) or various doses of aqueous FAI extracts(1-4 g/kg) for 14 d. Transit function was assessed using an ink propulsion test. Rats were then sacrificed,and the ultramicrostructure of colonic tissue was examined using transmission electron microscopy. The expression of the 5-hydroxytryptamine receptor 4(5-HTR4) and neurofilament-H was assessed in colon tissues using real-time PCR,Western blot,and immunohistochemistry.RESULTS: Mosapride and high dose(4 g/kg) of aqueous FAI extracts significantly improved the bowel movement in cathartic colons compared to untreated model colons as measured by the intestinal transit rate(70.06 ± 7.25 and 72.02 ± 8.74,respectively,vs 64.12 ± 5.19; P < 0.05 for both). Compared to controls,the ultramicrostructure of cathartic colons showed signsof neural degeneration. Treatment with mosapride and aqueous FAI extracts resulted in recovery of ultrastructural pathology. Treatment with mosapride alone upregulated the gene and protein expression of 5-HTR4 compared to untreated controls(P < 0.05 for both). Treatment with aqueous FAI extracts(≥ 2 g/kg) increased 5-HTR4 m RNA levels(P < 0.05),but no change in protein level was observed by Western blot or immunohistochemistry. The m RNA and protein levels of neurofilament-H were significantly increased with mosapride and ≥ 2 g/kg aqueous FAI extracts compared to controls(P < 0.05 for all).CONCLUSION: Aqueous FAI extracts and mosapride strengthen bowel movement in cathartic colons via increasing the expression of 5-HTR4 and neurofilament-H. | Shi-Yi Wang Yan-Ping Liu Yi-Hong Fan Lu Zhang Li-Jun Cai Bin Lv | 2015 | World Journal of Gastroenterology2015，21，31： | 11 |
| 7 | Characteristics analysis on high density spatial sampling seismic data显示文摘中国的大陆人免职盆被复杂地质的结构和各种各样的水库岩性学描绘。因此,高精确探索方法被需要。高密度空间采样是一种新技术增加地震探索的精确性。我们简短讨论点来源和接收装置技术,分析高密度在 situ 方法的空间采样,介绍吉季斯· J · O 介绍的对称的采样原则。Vermeer,并且讨论高密度从波浪地连续性的观点的空间采样技术。我们强调高密度的分析空间采样特征,包括高密度,首先,裂缝为近的表面结构的调查有利,改善静态的修正精确,在到增加的短偏移量的稠密的接收装置间距的使用在浅深度的有效范围,和思考成像的精确性。协调噪音不是 aliased 和噪音分析精确,抑制作为结果增加。空间采样提高的高密度各种各样的数学变换的波浪地连续性和精确性,它受益飘动地分离。最后,我们指出空间采样技术是的高密度的困难的部分处理的数据。更多的研究需要在分析并且处理地震数据的巨大的数量的方法上被做。 | Cai Xiling Liu Xuewei Deng Chunyan Lv Yingme | 2006 | Applied Geophysics2006，3，1： | 11 |
| 8 | Potential roles of EZH2, Bmi-1 and mi R-203 in cell proliferation and invasion in hepatocellular carcinoma cell line Hep3B显示文摘AIM: To investigate the potential roles of enhancer of zeste homolog2(EZH2), Bmi-1 and mi R-203 in cell proliferation and invasion in hepatocellular carcinoma(HCC) cell line Hep3 B.METHODS: A total of 73 patients who underwent surgical resection at Fuzong Clinical Medical College of Fujian Medical University were enrolled in this study. Hep3 B cells were cultivated in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37?℃. Vectors that containing c DNA of the EZH2 gene or mi R-203 targeted sh RNA plasmid were constructed, and then transfected into Hep3 B cells. The m RNA expression of mi R-203, EZH2, and Bmi-1 was analyzed using quantitative real-time polymerase chain reaction analysis, and the protein levels of EZH2 and Bmi-1 were detected by Western blot analysis. Effect of EZH2 or mi R-203 on cell proliferation was observed by methyl thiazolyl tetrazolium assay, and cell apoptosis was assessed using flow cytometry. Besides, effect of EZH2 or mi R-203 on tumor cell invasion was detected using Transwell assay.RESULTS: The m RNA levels of EZH2 and Bmi-1 in HCC tissues and in Hep3 B cells were significantly higher compared with those in normal samples(P < 0.01), while mi R-203 level was significantly lower in HCC tissues(P < 0.01). Hep3 B cells transfected with EZH2-sh RNA or mi R-203-sh RNA showed lower expression levels of EZH2 and Bmi-1(P < 0.05). Compared with controls, Hep3 B cells transfected with EZH2-sh RNA had relative slow cell proliferation, indicating that low expression of EZH2 and Bmi-1 and overexpression of mi R-203 could inhibit Hep3 B cell proliferation(P < 0.05). The average apoptosis rate of Hep3 B cells transfected with EZH2-sh RNA vector was about 18.631%, while that of Hep3 B cells transfected with sh RNA vector was about 5.33%, suggesting that EZH2 was down-regulated by transfecting with EZH2-sh RNA, and the down-regulated EZH2 contributed to the cell apoptosis. Low expression of EZH2 and Bmi-1 and overexpression of mi R-203 could reduce Hep3 B cell invasion(P < 0.05).CONCLUSION: Our study suggests that EZH2 and Bmi-1 are up-regulated while mi R-203 is downregulated in Hep3 B cells. Mi R-203 may contribute to the metastasis and enhance apoptosis of HCC cells by regulating EZH2 and Bmi-1. Our study may provide a theoretical basis for metastasis of HCC and targeted therapy of HCC. | Fang Yang Li-Zhi Lv Qiu-Cheng Cai Yi Jiang | 2015 | World Journal of Gastroenterology2015，21，47： | 11 |
| 9 | The effect of a seasonal stratification variation on the load exerted by internal solitary waves on a cylindrical pile显示文摘On the basis of Morison's empirical formula and modal separation method in estimating the force and torque exerted by internal solitary waves(ISWs) on a cylindrical pile, it is found that the loads exerted by the ISWs change largely in different seasons at the same site of the continental shelf in the South China Sea(SCS) even under the condition that the amplitudes of ISWs are the same. Thus, the effect of a seasonal water stratification variation on the force and torque exerted by the ISWs is investigated, and a three-parameter stratification model is employed. It is shown that the loads exerted by the ISWs depend largely on the water stratification. The stronger the water stratification, the larger the force and the torque; when the depth where the maximum thermocline appears is deepened, the force decreases but the torque increases; when the width of the thermocline is narrowed, the force increases but the torque decreases. The seasonal variation of the force and the torque exerted by the ISWs in four seasons in the SCS is thus explained. | CAI Shuqun XU Jiexin CHEN Zhiwu XIE Jieshuo DENG Xiaodong LV Haibin | 2014 | Acta Oceanologica Sinica2014，33，7： | 9 |
| 10 | Hard X-ray Imager (HXI) onboard the ASO-S mission显示文摘Hard X-ray Imager(HXI)is one of the three scientific instruments onboard the Advanced Spacebased Solar Observatory(ASO-S)mission,which is proposed for the 25th solar maximum by the Chinese solar community.HXI is designed to investigate the non-thermal high-energy electrons accelerated in solar flares by providing images of solar flaring regions in the energy range from 30 keV to 200 keV.The imaging principle of HXI is based on spatially modulated Fourier synthesis and utilizes about 91 sets of bi-grid sub-collimators and corresponding LaBr3 detectors to obtain Fourier components with a spatial resolution of about 3 arcsec and a time resolution better than 0.5 s.An engineering prototype has been developed and tested to verify the feasibility of design.In this paper,we present background,instrument design and the development and test status of the prototype. | Zhe Zhang Deng-Yi Chen Jian Wu Jin Chang Yi-Ming Hu Yang Su Yan Zhang Jian-Ping Wang Yao-Ming Liang Tao Ma Jian-Hua Guo Ming-Sheng Cai Yong-Qiang Zhang Yong-Yi Huang Xiao-Yan Peng Zong-Bin Tang Xuan Zhao Hong-He Zhou Lian-Guo Wang Jing-Xing Song Miao Ma Guang-Zhou Xu Jian-Feng Yang Di Lu Ying-Hong He Jin-You Tao Xiao-Long Ma Bao-Gang Lv Yan-Ping Bai Cai-Xia Cao Yu Huang Wei-Qun Gan | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 9 |
| 11 | HIV and intestinal parasite co-infections among a Chinese population: an immunological profile显示文摘Background:Parasite infections often result in a switch of the human body’s predominant immune reaction from T-helper 1(Th1)-type to Th2-type.Hence,parasite infections are widely expected to accelerate the progression of human immunodeficiency virus(HIV)infections to acquired immunodeficiency syndrome(AIDS).In the People’s Republic of China,both parasitic diseases and AIDS are epidemic in certain rural areas,and co-infections are relatively common.However,no population-based studies have yet investigated the frequency of HIV and parasite co-infections,and its effects on immune responses.We studied(1)the immune status of an HIV-infected population,and(2)the effect of co-infection of HIV and intestinal parasites on selected parameters of the human immune system.Methods:A total of 309 HIV-infected individuals were recruited and compared to an age-matched and sex-matched control group of 315 local HIV-negative individuals.Questionnaires were administered to all participants to obtain information on sociodemographic characteristics,sanitation habits,family income,and recent clinical manifestations.Two consecutive stool samples and 10 ml samples of venous blood were also collected from each individual for the diagnosis of parasite infections and quantitative measurements of selected cytokines and CD4+T-lymphocytes,respectively.Results:During the study period,79 HIV-infected individuals were not under highly active antiretroviral therapy(HAART)and were thus included in our analysis;the prevalence of intestinal helminth infections was 6.3%and that of protozoa was 22.8%.The most common protozoan infections were Blastocystis hominis(B.hominis)(13.9%)and Cryptosporidium spp.(10.1%).The prevalence of Cryptosporidium spp.in HIV-infected individuals was significantly higher than that in HIV negative individuals(P<0.05).Compared to the non-co-infected population,no significant difference was found for any of the measured immunological indicators(P>0.05).However,the following trends were observed:IFN-γlevels were lower,but the IL-4 level was higher,in the population co-infected with HIV and helminths.In the population co-infected with HIV and B.hominis,the IL-2 level was higher.The population co-infected with HIV and Cryptosporidium spp.had markedly lower CD4+T-lymphocyte counts.Conclusion:According to the immunologic profile, co-infection with helminths is disadvantageous to HIV-infected individuals. It was associated with a shift in the Th1/Th2 balance in the same direction as that caused by the virus itself, which might indicate an acceleration of the progress from an HIV infection to AIDS. Co-infection with Cryptosporidium spp. was not associated with a significant change in immune factors but co-infection with Cryptosporidium spp. was associated with a reduced level of CD4 + T-lymphocytes, confirming the opportunistic nature of such infections. Co-infection with B. hominis, on the other hand, was associated with an antagonistic shift in the immunological profile compared to an HIV infection. | Li-Guang Tian Tian-Ping Wang Shan Lv Feng-Feng Wang Jian Guo Xiao-Mei Yin Yu-Chun Cai Mary Kathryn Dickey Peter Steinmann Jia-Xu Chen | 2013 | Infectious Diseases of Poverty2013，2，1： | 8 |
| 12 | Nasopharyngeal adenoid cystic carcinoma: magnetic resonance imaging features in ten cases显示文摘Nasopharyngeal adenoid cystic carcinoma (NACC) is a rare malignancy with high local invasiveness. To date, there is no consensus on the imaging characteristics of NACC. To address this, we retrospectively reviewed 10 cases of NACC and summarized the magnetic resonance imaging (MRI) features. MR images of 10 patients with histologically validated NACC were reviewed by two experienced radiologists. The location, shape, margin, signal intensity, lesion texture, contrast enhancement patterns, local invasion, and cervical lymphadenopathy of all tumors were evaluated. Clinical and pathologic records were also reviewed. No patients were positive for antibodies against Epstein-Barr virus (EBV). The imaging patterns of primary tumors were classified into two types as determined by location, shape, and margin. Of all patients, 7 had tumors with a type 1 imaging pattern and 3 had tumors with a type 2 imaging pattern. The 4 tubular NACCs were all homogeneous tumors, whereas 3 (60%) of 5 cribriform NACCs and the sole solid NACC were heterogeneous tumors with separations or central necrosis on MR images. Five patients had perineural infiltration and intracranial involvement, and only 2 had cervical lymphadenopathy. Based on these results, we conclude that NACC is a local, aggressive neoplasm that is often negative for EBV infection and associated with a low incidence of cervical lymphadenopathy. Furthermore, MRI features of NACC vary in locations and histological subtypes. | Xue-Wen Liu Chuan-Miao Xie Hui Li Rong Zhang Zhi-Jun Geng Yun-Xian Mo Jing Zhao Mu-Yan Cai Yan-Chun Lv Pei-Hong Wu | 2012 | Chinese Journal of Cancer2012，31，1： | 8 |
| 13 | Case report of primary splenic angiosarcoma with hepatic metastases显示文摘Primary splenic angiosarcoma(PSA) is the most unusual type of malignancy with early multifocal metastasis through hematogenous spread. PSA is generally believed to originate from splenic sinusoidal vascular endothelium with a high rate of metastasis and to have a poor prognosis. Its etiology and pathogenetic mechanisms have not yet been clearly described. Thus far, only approximately 200 cases have been reported. PSA has variable symptomatology with the potential to present with life-threatening complications. The diagnosis of PSA is challenging; and often late. PSA should be considered in the differential diagnosis of patients with splenomegaly and anemia of unknown etiology. Surgical treatment with splenectomy is considered the only curative intervention for potential long-term disease-free survival. Early diagnosis and treatment are very important. It is important that clinical doctors improve the understanding of PSA. Herein, we report one rare case of PSA with hepatic metastases, along with a review of the current literature. | Fang Chen Hai-Feng Jin Yi-Hong Fan Li-Jun Cai Zhuo-Yi Zhang Bin Lv | 2015 | World Journal of Gastroenterology2015，21，39： | 8 |
| 14 | Detection of Atrazine Residue in Food Samples by a Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay显示文摘Atrazine(AT,2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine)has been detected in ground water in several areas of the United States for many years,as well as in China,wherein the growth rate of its | LV Zhi Qiang WANG Cai Hong WANG Ting Ting CHEN Cui Cui WANG Ying NING Bao An LIU Ming LIU Jian Qing BAI Jia Lei PENG Yuan GAO Zhi Xian | 2013 | Biomedical and Environmental Sciences2013，26，5： | 5 |
| 15 | Structural shift of gut microbiota during chemopreventive effects of epigallocatechin gallate on colorectal carcinogenesis in mice显示文摘AIM To investigate the effect of epigallocatechin gallate(EGCG) on structural changes of gut microbiota in colorectal carcinogenesis.METHODS An azoxymethane(AOM)/dextran sodium sulfate(DSS)-induced colitis mouse model was established. Fortytwo female FVB/N mice were randomly divided into the following three groups: group 1(10 mice, negative control) was treated with vehicle, group 2(16 mice, positive control) was treated with AOM plus vehicle, and group 3(16 mice, EG) was treated with AOM plus EGCG. For aberrant crypt foci(ACF) evaluation, the colons were rapidly took out after sacrifice, rinsed with saline, opened longitudinally, laid flat on a polystyrene board, and fixed with 10% buffered formaldehyde solution before being stained with 0.2% methylene blue in saline. For tumor evaluation, the colon was macroscopically inspected and photographed, then the total number of tumors was enumerated and tumor size measured. For histological examination, the fixed tissues were paraffin-embedded and sectioned at 5 mm thickness. Microbial genomic DNA was extracted from fecal and intestinal content samples using a commercial kit. The V4 hypervariable regions of 16 S r RNA were PCR-amplified with the barcoded fusion primers. Using the best hit classification option, the sequences from each sample were aligned to the RDP 16 S r RNA training set to classify the taxonomic abundance in QIIME. Statistical analyses were then performed.RESULTS Treatment of mice with 1% EGCG caused a significant decrease in the mean number of ACF per mouse, when compared with the model mice treated with AOM/DSS(5.38 ± 4.24 vs 13.13 ± 3.02, P < 0.01). Compared with the positive control group, 1% EGCG treatment dependently decreased tumor load per mouse by 85%(33.96 ± 6.10 vs 2.96 ± 2.86, respectively, P < 0.01). All revealed that EGCG could inhibit colon carcinogenesis by decreasing the number of precancerous lesions as well as solid tumors, with reduced tumor load and delayed histological progression of CRC. During the cancerization, the diversity of gut microbiota increased, potential carcinogenic bacteria such as Bacteroides were enriched, and the abundance of butyrate-producing bacteria(Clostridiaceae, Ruminococcus, etc.) decreased continuously. In contrast, the structure of gut microbiota was relatively stable during the intervention of EGCG on colon carcinogenesis. Enrichment of probiotics(Bifidobacterium, Lactobacillu, etc.) might be a potential mechanism for EGCG's effects on tumor suppression. Via bioinformatics analysis, principal coordinate analysis and cluster analysis of the tumor formation process, we found that the diversity of gut microbiota increased in the tumor model group while that in the EGCG interfered group(EG) remained relatively stable.CONCLUSION Gut microbiota imbalance might be a potential mechanism for the prevention of malignant transformation by EGCG, which is significant for diagnosis, treatment, prognosis evaluation, and prevention of colorectal cancer. | Xin Wang Tao Ye Wen-Jie Chen You Lv Zong Hao Jun Chen Jia-Ying Zhao Hui-Peng Wang Yuan-Kun Cai | 2017 | World Journal of Gastroenterology2017，23，46： | 5 |
| 16 | Corrosion resistance properties of AZ31 magnesium alloy after Ti ion implantation显示文摘Magnesium alloys have a wide range of applications in industry; however, their corrosion resistance, wear resistance, and hardness are rather poor, which limit their applications. Ti ion was implanted into the AZ31 magnesium alloy surface by metal vapor vacuum arc (MEVVA) implanter. This metal arc ion source has a broad beam and high current capabilities. The implantation energy was fixed at 45 keV and the dose was at 9 × 1017 cm?2. Through ion implantation, Ti ion implantation layer with approximately 900 nm in thickness was directly formed on the surface of AZ31 magnesium alloy, by which its surface property greatly improved. The chemical states of some typical elements of the ion implantation layer were analyzed by means of X-ray photoelectron spectroscopy (XPS), while the cross sectional morphology of the ion im-plantation layer and the phase structure were observed by means of scanning electron microscopy (SEM) and X-ray diffraction (XRD). The property of corrosion resistance of the Ti ion implanted layer was studied by the CS300P electrochemistry corrosion workstation in 3.5% NaCl solution. The results showed that the property of corrosion resistance was enhanced remarkably, while the corrosion velocity was obviously slowed down. | CHEN Fei ZHOU Hai CAI Suo LV Fanxiu LI Chengming | 2007 | Rare Metals2007，26，2： | 4 |
| 17 | Liver transplant for 70 patients with end-stage liver diseases显示文摘BACKGROUND: Liver transplantation has evolved as a successful treatment for patients with end-stage liver cirrhosis and acute liver failure. Postoperative survival rates have increased to 90% in 1 year and 80% in 5 years as a result of improvements in immunosuppression, perioperative management and surgical techniques. However, a wide range of postoperative complications are of technical or medical origin. This study was undertaken to determine the relationship between the technical improvements and optimal timing of surgery and its outcome. METHODS: From April 1999 to October 2005, typical orthotopic or piggyback liver transplantation was performed in 70 patients (58 men and 12 women, aged 19-74 years). Twenty-four patients had liver carcinoma and cirrhosis, and 46 had benign liver disease. RESULTS: All patients survived the operation and 14 died in the first month after surgery because of respiratory failure (6), respiratory failure accompanied by acute renal failure (4), intra-abdominal hemorrhage and infection (2), and cerebral edema (2). A total of 76 complications occurred in the 70 patients after operation: pneumonia (34), right pleural effusion (11), bile leakage (7), postoperative intra-abdominal hemorrhage and infection (4), acute renal failure (4), acute rejection (3), wound infection (2), biliary tract stenosis (2), severe cholangitis derived from cholelith (2), morphological alteration of biliary tree (2), cerebral edema (2), empyema (1), chronic rejection (1), and wound hematoma (1). Finally, 33 patients survived more than 6 months, 16 more than 1 year, 4 more than 2 years, and 2 more than 6 years after operation. The perioperative survival rate was 80% in this series.CONCLUSIONS: Liver transplantation is an effective treatment for patients with end-stage liver disease. To obtain good results, improvements of surgical technique, optimal timing and better postoperative care are needed. | Jiang, Yi Lv, Li-Zhi Cai, Qiu-Cheng Zhang, Kun Hu, Huan-Zhang Zhang, Shao-Geng Yang, Fang Wei, Wei-Ming Zhang, Xiao-Jin Chen, Yong-Biao Pan, Fan | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，1： | 4 |
| 18 | Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse显示文摘Drugs can cause obvious damage to the brain. To verify the relationship between acupuncture, neurotrophic factor expression and brain cell structural changes, this study established a rat model of heroin relapse using intramuscular injection of increasing amounts of heroin. During the detoxification period, rat models received acupuncture at Baihui(DU20) and Dazhui(DU14). Electron microscopy demonstrated that the structure of the ventral tegmental area in heroin relapse rats gradually became normalized after acupuncture treatment. Immunohistochemical staining exhibited that the expression of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor increased in the ventral tegmental area following acupuncture. Moreover, the effects were similar to that of methadone, a type of medicine called an opioid. Results suggested that acupuncture at Baihui and Dazhui protected brain neurons against injury in rats with heroin relapse by promoting brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. | Rongjun Zhang Xinghui Cai Xiaoge Song Chaoyang Dong Xiaorong Hou Lei Lv | 2014 | Neural Regeneration Research2014，9，3： | 4 |
| 19 | Hot deformation behavior and microstructure evolution of a Mg–Gd–Nd–Y–Zn alloy显示文摘The hot deformation behavior of homogenized Mg–6.5Gd–1.3Nd–0.7Y–0.3Zn alloy was investigated during compression at temperatures of 250–400 ℃ and at strain rates ranging from 0.001 to 0.100 s^(-1). Microstructure analyses show that the flow behaviors are associated with the deformation mechanisms. At the lower temperatures(250–300 ℃), deformation twinning is triggered due to the difficult activation of dislocation cross-slip. Dynamic recrystallization(DRX) accompanied by dynamic precipitation occurs at the temperature of 350 ℃ and influences the softening behavior of the flow.DRX that develops extensively at original grain boundaries is the main softening mechanism at the high temperature of 400 ℃ and eventually brings a more homogeneous microstructure than that in other deformation conditions. The volume fraction of the DRXed grains increases with temperature increasing and decreases with strain rate increasing. | Xiu-Li Hou Yan Li Peng Lv Jie Cai Le Ji Qing-Feng Guan | 2016 | Rare Metals2016，35，7： | 4 |
| 20 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员