| 1 | A Preliminary Study on Combination Therapy of Artemisinin Dimer Oxime and Topotecan against Nonsmall Cell Lung Cancer in Mice显示文摘Background: Artemisinin dimer oxime – dimer molecule synthesized from artemisinin possesses high bioavailability and marked in vitro anticancer activities against solid tumor?derived cell lines, endothelial cell proliferation, migration, and angiogenic processes. Numerous murine models have been developed to study human cancer. The most widely used models are the human tumor xenograft mouse model. Materials and Methods: In this study, human tumor cells(NCI?H640, 1 × 107 in 100 μL) are implanted subcutaneously, or 1 × 107 in 50 μL in the thoracic cavity, in athymic nude mice(nu/nu). The implanted cells were allowed to grow for 10 days before initiation of drug treatment(dimer oxime and topotecan, ip). Tumor volume and thoracic/body weight ratio were recorded. Results: We successfully established subcutaneous and thoracic xenografts with human nonsmall cell lung cancer cell line xenografts in athymic nude mice in only 10 days. Using these models, we attempted treatment of xenografts with topotecan – a known anticancer drug and artemisinin dimer oxime or combination of these two drugs. Combination therapy showed a significant reduction in tumor volume and tumor/body weight. Treatments with combination of topotecan and dimer oxime resulted in the reduced mortality rates in comparison with untreated mice. Conclusions: Xenograft tumor models are useful for preclinical screening of new pharmacophores. From this preliminary study, it appears that combination of dimer oxime and topotecan may be used as chemotherapeutic agents against nonsmall cell lung cancer. Further studies are needed to evaluate other combination treatment regimens as well as the mechanism(s) of action. | Mohammad K.Ashfaq Mohamed Sadek Abdel-Bakky Mir Tahir Maqbool Waseem Gul Mahmoud A.ElSohly | 2018 | World Journal of Traditional Chinese Medicine2018，4，1： | 3 |
| 5 | Influence of rangeland protection and seasonal grazing on aboveground vegetation,forage quality and weight gain of small ruminants–a study in Thar Desert,Pakistan显示文摘The Thar Desert,Sindh,Pakistan is characterized by low productivity.Besides,economy is based on agriculture,livestock and mining,nevertheless,livestock graze freely on public and private land.The aim of this research was to determine biomass production and to evaluate the effects of continuous and seasonal grazing on protected and unprotected plots.A 45 ha protected rangeland area of Hurrabad in the Umerkot Thar desert was selected and divided into three blocks of 15 ha each.Blocks of the same size were also established in unprotected area.The data for vegetation biomass,canopy cover,forage nutrients and weight gain of animals in two seasons(spring and summer)was collected from both protected and unprotected sites.The results showed that biomass significantly increased in summer in both sites.However,the biomass values in protected sites were significantly higher.Similarly,the vegetation cover also seemed to increase in summer in both protected(90.7%±0.29%)and unprotected sites(39.2%±0.09%).The foliar concentrations of all nutrients varied significantly with season.The average final live-weight gain for does on the protected grazing sites during the 42-day period in spring and the 96 days after the monsoon was almost double that of does grazing on the unprotected site during 2016 and 2017(P<0.05).The study concludes that the protection of grazing lands during certain periods can lead to better production of vegetation and livestock and improve range conditions. | ISLAM Muhammad RAZZAQ Abdul HASSAN Sawsan ZUBAIR Muhammad KALROO Muhammad Waseem KHAN Attaullah GUL Shamim AHMAD Sarfraz RISCHKOWSKY Barbara Ann LOUHAICHI Mounir | 2023 | Journal of Mountain Science2023，20，2： | 0 |
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