| 1 | Mechanism of improving skin barrier function in a mouse model of atopic dermatitis using Mahuang Lianqiao Chixiaodou decoction and its disassembled formula显示文摘Objective: To reveal the mechanism of Mahuang Lianqiao Chixiaodou decoction and its disassembled formula for improving the skin barrier function in a mouse model of atopic dermatitis(AD).Methods: Sixty specific-pathogen free male BALB/c mice were randomly divided into the control group,model group, whole formula group(WF), exterior-releasing formula group(ERF), interior-clearing formula group(ICF), and positive control group(PC). A mouse model of AD was established using the semiantigen 2,4-dinitrofluorobenzene induction method. The lesion scores, transepidermal water loss and p H, and skin histopathology of mice in each group were observed. The expressions of filaggrin, loricrin,and involucrin were detected by the streptavidin peroxidase immunohistochemical method and western blotting, and their mRNA expressions were detected by quantitative polymerase chain reaction.Results: Mice in the WF, ERF, ICF, and PC groups showed reduced skin lesion performance, improved histopathology, decreased skin lesion score, transepidermal water loss and pH, and upregulated expressions of proteins including filaggrin, loricrin, and involucrin, and their mRNAs. The most obvious regulatory effect was observed in the WF group, followed by the ICF, ERF, and PC groups, accordingly.Conclusions: Mahuang Lianqiao Chixiaodou decoction and its disassembled formula can improve the skin barrier function in a mouse model of AD by upregulating filaggrin, loricrin, and involucrin, and their mRNA expressions, and the most optimal effect was noted in the WF group, followed by the ICF and ERF groups, which suggests that the effect of clearing heat and resolving dampness in improving the skin barrier function of AD is more obvious and is one of the key treatments for AD. | Jingang Liu Yan Sun Huimin Yuan Yuda Li Jing Feng Yushan Gao Yang Tang Shujing Zhang Hang Wang Fengjie Zheng | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，4： | 2 |
| 6 | Identification and functional comparison of Bcl2 splicing isoforms in mouse embryonic stem cells显示文摘Embryonic stem cells(ESCs)provide an ideal model for investigating developmental processes and are great sources for developing regenerative m edicine.Harnessing apoptosis facilitates accurate recapitulation of signalling events during em bryogenesis and allows efficient expansion of the ESCs during differentiation.Bcl2,a key regulator of intrinsic anti-apoptotic pathway,encodes two splicing isoforms.However,the identification and functional comparison of Bcl2 splicing isoforms in mouse ESCs(mESCs)remains to be elucidated.Here,we provide the evidence that both Bcl2 splicing variants are expressed in mESCs.Despite the structural difference,they have similar subcellular localization. | Wang Xueyue Wang Jiaqi Cheng Yuda Wang Jiali Tian Yanping Zhang Junlei Liu Gaoke Xu Yixiao Liu Lianlian Ruan Yan Jian Rui | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 7 | In Situ Saturating Mutagenesis Screening Identifies a Functional Genomic Locus that Regulates Ucp1 Expression显示文摘A better understanding of the molecular mechanisms that control the UCP1 expression in brown and beige adipocytes is essential for us to modulate adipose cell fate and promote thermogenesis,which may provide a therapeutic view for the treatment of obesity and obesity-related diseases.To systematically identify cis-element(s)that transcriptionally regulates Ucp1,we here took advantage of the high-throughput CRIPSR-Cas9 screening system,and performed an in situ saturating mutagenesis screen,by using a customized sgRNA library targeting the~20 kb genomic region near Ucp1.Through the screening,we have identified several genomic loci that may contain key regulatory element for Ucp1 expression in cultured brown and white adipocytes in vitro,and in inguinal white adipose tissue in vivo.Our study highlights a broadly useful approach for studying cis-regulatory elements in a high-throughput manner. | Yan Qiu Xiaojian Liu Yingmin Sun Shuang Li Yuda Wei Cheng Tian Qiurong Ding | 2021 | Phenomics2021，1，1： | 0 |
| 8 | Development of a heart rate variability and complexity model in predicting the need for life-saving interventions amongst trauma patients显示文摘Background:Triage trauma scores are utilised to determine patient disposition,interventions and prognostication in the care of trauma patients.Heart rate variability(HRV)and heart rate complexity(HRC)reflect the autonomic nervous system and are derived from electrocardiogram(ECG)analysis.In this study,we aimed to develop a model incorporating HRV and HRC,to predict the need for life-saving interventions(LSI)in trauma patients,within 24 h of emergency department presentation.Methods:We included adult trauma patients(≥18 years of age)presenting at the emergency department of Singapore General Hospital between October 2014 and October 2015.We excluded patients who had non-sinus rhythms and larger proportions of artefacts and/or ectopics in ECG analysis.We obtained patient demographics,laboratory results,vital signs and outcomes from electronic health records.We conducted univariate and multivariate analyses for predictive model building.Results:Two hundred and twenty-five patients met inclusion criteria,in which 49 patients required LSIs.The LSI group had a higher proportion of deaths(10,20.41%vs 1,0.57%,p<0.001).In the LSI group,the mean of detrended fluctuation analysis(DFA)-α1(1.24 vs 1.12,p=0.045)and the median of DFA-α2(1.09 vs 1.00,p=0.027)were significantly higher.Multivariate stepwise logistic regression analysis determined that a lower Glasgow Coma Scale,a higher DFA-α1 and higher DFA-α2 were independent predictors of requiring LSIs.The area under the curve(AUC)for our model(0.75,95%confidence interval,0.66–0.83)was higher than other scoring systems and selected vital signs.Conclusions:An HRV/HRC model outperforms other triage trauma scores and selected vital signs in predicting the need for LSIs but needs to be validated in larger patient populations. | Aravin Kumar Nan Liu Zhi Xiong Koh Jayne Jie Yi Chiang Yuda Soh Ting Hway Wong Andrew Fu Wah Ho Takashi Tagami Stephanie Fook-Chong Marcus Eng Hock Ong | 2019 | Burns & Trauma2019，7，1： | 0 |
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