| 2 | Effect of Mono-2-ethyhexyl Phthalate on DNA Methylation in Human Prostate Cancer LNCaP Cells显示文摘Objective To evaluate whether mono(2‐ethylhexyl) phthalate(MEHP) affects genomic DNA methylation and the methylation status of some specific genes such as patched gene(PTCH) and smoothened gene(SMO) in LNCaP cells. Methods LNCaP cells were treated with MEHP(0, 1, 5, 10, and 25 μmol/L) for 3 days. An ELISA assay was preformed to detect genomic methylation, including 5‐methylcytosine(5‐mC) and 5‐hydroxymethylcytosine(5‐hmC) content. A pyrosequencing assay was applied to assess DNA methylation in PTCH and SMO gene promoters. The correlation between DNA methylation and gene expression was assessed. Results The proportion of cytosines with 5‐mC methylation in LNCaP cells was significantly decreased by MEHP(1, 5, 10, and 25 μmol/L) in a dose‐dependent manner(P < 0.01). For genes in the Hedgehog pathway, there was no significant MEHP concentration‐dependent difference in the DNA methylation of PTCH and SMO. Conclusion MEHP might affect the progression of prostate cancer through its effect on global DNA methylation. | WU Jian Hui CHEN Jiao WANG Yong XIA Bin WANG Rong ZHAO Yan WANG Qin Xia SONG Qi YAO Shun Heng ZHANG Yun Hui SUN Zu Yue | 2017 | Biomedical and Environmental Sciences2017，30，9： | 1 |
| 3 | Discrete element method study of solid descending and residence properties in COREX shaft furnace with center gas supply device显示文摘Solid descending and residence properties in COREX shaft furnace with a center gas supply device (CGSD) were studied using the discrete element method. Solid flow pattern, residence time distribution, and local solid residence time (SRT) were analyzed. Moreover, the effects of the rotation speed of screws, the radius, and height of CGSD were investigated. The results show that the solid flow is dominantly a plug flow in the shaft furnace with the CGSD. In the upper region of the shaft furnace for gas-solid reduction, uniform distribution of local SRT is observed, which is preferable to match the even gas distribution expected due to the CGSD and slots. For different rotation speeds of the screws, the distribution of solid flow patterns is similar. The averaged residence time, dispersed plug volume fraction, and dead volume fraction decrease with increasing the rotation speed of the screws. The radius of the CGSD affects the solid residence in the shaft furnace. Local SRT above the CGSD increases with the increase in the radius of CGSD, possibly leading to the formation of more agglomerates. Hence, the radius of CGSD should be chosen carefully. The height of CGSD has a minor effect on solid residence in the shaft furnace. | Heng Zhou Qin-fu Hou Ming-yin Kou Shun Yao Sheng-li Wu | 2019 | Journal of Iron and Steel Research(International)2019，26，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员