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| 1 | Cold-chain transportation in the frozen food industry may have caused a recurrence of COVID-19 cases in destination:Successful isolation of SARS-CoV-2 virus from the imported frozen cod package surface显示文摘Coronavirus disease 2019(COVID-19)pandemic has spread in 220 countries/regions towreak havoc to human beings around theworld.At present,the second wave of COVID-19 has begun in many European countries.The complete control of COVID-19 is very urgent.Although China quickly brought the virus under control,there have been eight sporadic outbreaks in China since then.Both in Xinfadi of Beijing and Dalian outbreak of COVID-19,environmental swab samples related to imported cold chain food were tested nucleic acid positive for SARS-CoV-2.In this outbreak in Qingdao,we directly isolated SARS-CoV-2 from the cod outer package's surface swab samples.This is the first time worldwide,SARS-CoV-2 were isolated from the imported frozen cod outer package's surface,which showed that imported frozen food industry could import SARS-CoV-2 virus. | Peipei Liu Mengjie Yang Xiang Zhao Yuanyuan Guo Liang Wang Jing Zhang Wenwen Lei Weifang Han Fachun Jiang William J.Liu George F.Gao Guizhen Wu | 2020 | Biosafety and Health2020，2，4： | 41 |
| 2 | Analysis of pesticide residues in commercially available chenpi using a modified QuEChERS method and GC-MS/MS determination显示文摘To ensure the safety of the commercially available chenpi,a convenient and fast analytical method was developed for the determination of 133 pesticide residues in chenpi using gas chromatography-tandem mass spectrometry(GC-MS/MS).In this study,different extraction solvents,redissolution solvents and adsorbents were tested according to the recovery and purification effect to obtain a modified QuEChERS method.The samples were extracted with acetonitrile.During the clean-up step,octadecyl-modified silica(C18)and graphitized carbon black(GCB)were selected,and aminopropyl(NH2)was used instead of primary secondary amine(PSA)because of its weaker ion exchange capacity which had little effect on the recovery of ditalimfos.Samples were quantified by matrix-matched calibration with internal standards.All pesticides showed good linearity in the respective range,both with values of r2>0.99.The average recoveries of the pesticides spiked samples ranged from 70.0% to 112.2% with the RSDs of 0.2%–14.4%.The modified QuEChERS method was validated and applied to twenty real samples.Five pesticides were found in eight batches,but no pesticide exceeded the maximum residue limits(MRL,MRL reference to European commission). | Shuang Li Peipei Yu Ceng Zhou Ling Tong Dongxiang Li Zhiguo Yu Yunli Zhao | 2020 | Journal of Pharmaceutical Analysis2020，10，1： | 34 |
| 3 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 4 | Assessment of diurnal variation of summer precipitation over the Qilian Mountains based on an hourly merged dataset from 2008 to 2014显示文摘调查日报在在向东北西藏的高原的 Qilian 山的夏天降水的变化,时时,为这个区域的降水数量在 0.1 点用一个时时合并的降水产品在 2008-2014 的夏天期间被分析 ? 湵慦潶慲汢? 諟D禎澞?汣浩瑡 ? 档湡敧椠?? | LIU Xuemei ZHANG Mingjun WANG Shengjie WANG Jie ZHAO Peipei ZHOU Panpan | 2017 | Journal of Geographical Sciences2017，27，3： | 14 |
| 5 | A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein显示文摘最新新兴的中东呼吸症候群 coronavirus (MERS-CoV ) 能在人引起严重、致命的急性呼吸疾病。尽管有全球努力,潜力为一联系在未来流行不能被排除。有效相对的措施的发展是迫切的。MERS-CoV-specific 抗病毒的药或疫苗是还没可得到的。使用 MERS-CoV (MERS-RBD ) 的尖铁受体绑定领域使老鼠免疫,我们识别了二抵销 monoclonal 抗体(mAbs ) 4C2 和 2E6。两 mAbs potently 与高功效在 vitro 绑在 MERS-RBD 和块病毒入口。我们进一步由使在 Fab 碎片和 RBD 之间的建筑群结晶调查了他们中立化的机制,并且解决了 4C2 Fab/MERS-RBD 建筑群的结构。结构证明 4C2 认出部分重叠的 epitope 在 MERS-RBD 的受体绑定脚印,从而由位的阻碍者和接口残余竞争防碍病毒 / 受体相互作用。2E6 也堵住受体绑定,并且为绑定与 4C2 竞争到 MERS-RBD。基于结构,我们进一步由保存仅仅 paratope 残余并且从人的免疫球蛋白与对应物代替留下的氨基酸使 4C2 人性化。人性化的 4C2 (4C2h ) 抗体支撑了类似的抵销的活动和生物化学的特征到父母老鼠抗体。最后,我们证明 4C2h 能显著地在感染 MERS-CoV 的 Ad5-hCD26-transduced 鼠标的肺消除病毒 titers,因此在临床的设置为预防和治疗代表一个有希望的代理人。 | Yan Li YuhuaWan Peipei Liu Jincun Zhao Guangwen Lu Jianxun Qi Qihui Wang Xuancheng LU Ying Wu Weniun Liu Buchang Zhang Kwok-Yung Yuen Stanley Perlman George F Gao Jinghua Yan | 2015 | Cell Research2015，25，11： | 14 |
| 6 | Long Distance Transmission of SARS-CoV-2 from Contaminated Cold Chain Products to Humans——Qingdao City,Shandong Province,China,September 2020显示文摘Summary What is already known about this topic?Though coronavirus disease 2019(COVID-19)has largely been controlled in China,several outbreaks of COVID-19 have occurred from importation of cases or of suspected virus-contaminated products.Though several outbreaks have been traced to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)isolated on the outer packaging of cold chain products,live virus has not been obtained. | Huilai Ma Zhaoguo Wang Xiang Zhao Jun Han Yong Zhang Hong Wang Cao Chen Ji Wang Fachun Jiang Jie Lei Jingdong Song Shaofeng Jiang Shuangli Zhu Huihui Liu Dongyan Wang Yao Meng Naiying Mao Yanhai Wang Zhen Zhu Zhixiao Chen Bingling Wang Qinqin Song Haijun Du Qun Yuan Dong Xia Zhiqiang Xia Peipei Liu Yuchao Wu Zijian Feng Ruqin Gao George F.Gao Wenbo Xu | 2021 | China CDC weekly2021，3，30： | 13 |
| 7 | Effects of different mixing ratios on emissions from passenger cars fueled with methanol/gasoline blends显示文摘Regulated and unregulated emissions from four passenger cars fueled with methanol/gasoline blends at different mixing ratios (M15,M20,M30,M50,M85 and M100) were tested over the New European Driving Cycle (NEDC).Volatile organic compounds (VOCs) were sampled by Tenax TA and analyzed by thermal desorption-gas chromatograph/mass spectrometer (TD-GC/MS).Carbonyls were trapped on dinitrophenylhydrazine (DNPH) cartridges and analyzed by high performance liquid chromatography (HPLC).The results showed that total emissions of VOCs and BTEX (benzene,toluene,ethylbenzene,p,m,o-xylene) from all vehicles fueled with methanol/gasoline blends were lower than those from vehicles fueled with only gasoline.Compared to the baseline,the use of M85 decreased BTEX emissions by 97.4%,while the use of M15 decreased it by 19.7%.At low-to-middle mixing ratios (M15,M20,M30 and M50),formaldehyde emissions showed a slight increase while those of high mixing ratios (M85 and M100) were three times compared with the baseline gasoline only.When the vehicles were retrofitted with new three-way catalytic converters (TWC),emissions of carbon monoxide (CO),total hydrocarbon (THC),and nitrogen oxides (NOx) were decreased by 24%-50%,10%-35%,and 24%-58% respectively,compared with the cars using the original equipment manufacture (OEM) TWC.Using the new TWC,emissions of formaldehyde and BTEX were decreased,while those of other carbonyl increased.It is necessary that vehicles fueled with methanol/gasoline blends be retrofitted with a new TWC.In addition,the specific reactivity of emissions of vehicles fueled with M15 and retrofitted with the new TWC was reduced from 4.51 to 4.08 compared to the baseline vehicle.This indicates that the use of methanol/gasoline blend at a low mixing ratio may have lower effect on environment than gasoline. | Hong Zhao Yunshan Ge Jianwei Tan Hang Yin Jiadong Guo Wei Zhao Peipei Dai | 2011 | Journal of Environmental Sciences2011，23，11： | 12 |
| 8 | Prognostic implications of epidermal growth factor receptor variant Ⅲ expression and nuclear translocation in Chinese human gliomas显示文摘Objective: To determine the prognostic implications and clinical significance of epidermal growth factor receptor variant Ⅲ(EGFRvⅢ) expression and EGFRvⅢ nuclear translocation in Chinese human gliomas.Methods: We retrospectively examined EGFRvⅢ expression and EGFRvⅢ nuclear translocation using immunohistochemistry in specimens of 240 Chinese patients with glioma, including 84 World Health Organization(WHO) II gliomas, 84 WHO Ⅲ gliomas and 72 glioblastomas(WHO IV). Factors that correlated with EGFRvⅢ and EGFRvⅢ nuclear translocation expression were analyzed by the Chi-square test. Kaplan-Meier methodology and Cox regression were used for the survival analysis.Results: Log-rank tests showed that patient age, Karnofsky performance scale(KPS) score, tumor grade,EGFRvⅢ expression, EGFRvⅢ nuclear translocation, 1 p/19 q codeletion, isocitrate dehydrogenase(IDH)mutation, Ki-67 labeling index and O6-methylguanine-DNA methyltransferase(MGMT) status(P<0.05) were significantly correlated with overall survival(OS) time. Multivariate Cox regression analysis revealed that patient age, tumor grade, EGFRvⅢ nuclear translocation, 1 p/19 q codeletion, and IDH mutation(P<0.05) were significantly correlated with OS. Patients with a high level of EGFRvⅢ nuclear translocation(≥7%) had both significantly shorter OS [hazard ratio(HR): 1.920, 95% confidence interval(95% CI): 1.228-3.003, P=0.004] and progression-free survival(PFS) times(HR: 1.661, 95% CI: 1.116-2.471, P=0.012) than those with a low level of EGFRvⅢ nuclear translocation(<7%).Conclusions: A high level of EGFRvⅢ nuclear translocation in glioma is an independent factor indicating a poor prognosis, but EGFRvⅢ expression is not an independent clinical prognostic factor. The level of EGFRvⅢ nuclear translocation maybe a novel and crucial prognostic biomarker in glioma. | Kaiyuan Yang Xiaohui Ren Liyuan Tao Peipei Wang Haihui Jiang Li Shen Yiming Zhao Yong Cui Mingxiao Li Song Lin | 2019 | Chinese Journal of Cancer Research2019，31，1： | 8 |
| 9 | Malware Detection Algorithm Based on the Attention Mechanism and ResNet显示文摘The cost of misclassifying a malware program as normal is often higher than that of misclassifying a normal program as malware.Therefore,how to improve the detection accuracy of malware programs is a very important problem.This paper proposes a deep learning malware program detection algorithm based on attention mechanism.Word2Vec model is used to map the Application programming interface(API)into word vectors,and all word vectors of each sample are arranged into a matrix with the same size.On this basis,residual network is used to extract features of samples.The features are input into the attention mechanism to learn the similarity between samples.Then,the features are weighted with the similarity to obtain the new features with better robustness.The new features and the original features are added element by element to obtain the sample features more suitable for classification.Finally,samples are classified by classifier.Experiments show that the classification effect of the proposed method is better than that of the traditional machine learning method. | WANG Lele WANG Binqiang ZHAO Peipei LIU Ruyi LIU Jiangang MIAO Qiguang | 2020 | Chinese Journal of Electronics2020，29，6： | 5 |
| 10 | Reemergent Cases of COVID-19--Xinjiang Uygur Autonomous Region,China,July 16,2020显示文摘Before the coronavirus disease 2019(COVID-19)outbreak on July 16,2020,no new COVID-19 cases were reported in Urumqi City of Xinjiang for 150 consecutive days.During this outbreak,826 confirmed COVID-19 cases have been reported in Urumqi.The phylogenetic characteristics of COVID-19 virus from clinical specimens of the initial 4 cases in the Urumqi outbreak were sequenced. | Cao Chen Hemuti Ma Zhiyuan Jia Xiang Zhao Dayan Wang Jun Zhao Zhenguo Gao Peipei Liu Yang Song Zhixiao Chen Yuchao Wu Yao Meng Guizhen Wu Wenbo Xu Xucheng Fan Yong Zhang | 2020 | China CDC weekly2020，2，39： | 5 |
| 11 | Anisotropic thermoelectric properties of layered compound SnSe2显示文摘Similar to high performance SnSe thermoelectrics, SnSe2 is also a layered structured semiconductor.However, its anisotropic thermoelectric properties are less experimentally investigated. In this work,Cl-doped SnSe2 bulk materials are successfully prepared, and their thermal stability and anisotropic transport properties are systematically studied. Unexpectedly, different from the theoretical prediction and other typical layered thermoelectric compounds like Bi_2Te_3, the out-of-plane zT_c value is higher than in-plane zT_a for the same composition. The zT value is significantly enhanced by Cl doping. A maximum zT_c of ~0.4 at 673 K is achieved in SnSe_(1.88)Cl_(0.12), twice higher than previously reported Cl-doped SnSe_2 synthesized by the solvothermal method. | Peipei Xu Tiezheng Fu Jiazhan Xin Yintu Liu Pingjun Ying Xinbing Zhao Hongge Pan Tiejun Zhu | 2017 | Science Bulletin2017，62，24： | 4 |
| 12 | Roles of low?density lipoproteinreceptor?related protein 1 in tumors显示文摘Low-density lipoprotein receptor-related protein 1(LRP1,also known as CD91),a multifunctional endocytic and cell signaling receptor,is widely expressed on the surface of multiple cell types such as hepatocytes,fibroblasts,neurons,astrocytes,macrophages,smooth muscle cells,and malignant cells.Emerging in vitro and in vivo evidence demonstrates that LRP1 is critically involved in many processes that drive tumorigenesis and tumor progression.For example,LRP1 not only promotes tumor cell migration and invasion by regulating matrix metalloproteinase(MMP)-2and MMP-9 expression and functions but also inhibits cell apoptosis by regulating the insulin receptor,the serine/threonine protein kinase signaling pathway,and the expression of Caspase-3.LRPI-mediated phosphorylation of the extracellular signal-regulated kinase pathway and c-jun N-terminal kinase are also involved in tumor cell proliferation and invasion.In addition,LRP1 has been shown to be down-regulated by microRNA-205 and methylation of LRP1CpG islands.Furthermore,a novel fusion gene,LRP1-SNRNP25,promotes osteosarcoma cell invasion and migration.Only by understanding the mechanisms of these effects can we develop novel diagnostic and therapeutic strategies for cancers mediated by LRP1. | Peipei Xing Zhichao Liao Zhiwu Ren Jun Zhao Fengju Song Guowen Wang Kexin Chen Jilong Yang | 2016 | Chinese Journal of Cancer2016，35，1： | 4 |
| 13 | Quantitative Prediction of Reduction in Large Pipe Setting Round Process显示文摘The control manner during the process to ensure the quality of pipe products mainly relies on the operator's experience, so it is very necessary to study the setting round process and obtain its spring-back law. The setting round process is shaping an oval section pipe into circular section, so it is difficult to provide a quantificational analysis for its spring-back process because of the curvature inequality of pipe section neutral layer. However, the spring-back law of the circle-oval process can be easily predicted. The experimental method is firstly used to establish the equivalent effect between the setting round process and the circle-oval process. The setting round process can be converted into the circle-oval process. There are two difficulties in the theoretical analysis for the circle-oval process: elastic-plastic bending problem of curved beam; statically indeterminate problem. A quantitative analytic method for the circle-oval process is presented on the basis of combination of the spring-back law of plane curved beam with the element dividing idea in finite element method. The ovality after unloading versus the relative reduction is plotted with analytical and experimental results respectively, which shows a fair agreement. Finally, the method of quantitative prediction of reduction for large pipe setting round is given based on the equivalent effect and the analytical results. Five pipes, which are needed to be set round, are used to carry out experiment so as to verify this method. The results of verification experiment indicates that, in the experimental range, the residual ovality are all under 0.35% after the once only setting round with the theoretical prediction reductions. It is much less than the 1% requirement of pipe standard. Applying the established theoretical analysis is able to correct the pipe ovality with sufficient accuracy, which provides theoretical direction to plant use. | ZHAO Jun ZHAN Peipei MA Rui ZHAI Ruixue | 2013 | Chinese Journal of Mechanical Engineering2013，26，4： | 4 |
| 14 | Genomic characterizations of H4 subtype avian influenza viruses from live poultry markets in Sichuan province of China, 2014–2015显示文摘Dear Editor,Avian influenza viruses (AIVs) have posed a serious threat to poultry production and public health. To date, more than fourteen AIV subtypes that are able to infect human beings have been documented. Also, it is suggested that new subtypes may be reported in the future, owing to the migration of wild birds and live poultry transportation (Gao, 2018).Poultry may act as a potential incubator for novel subtypes of avian influenza virus (Bi et al., 2016a; Bi et al., 2016b; Liu et al., 2014a; Su et al., 2017). Up to date, the H7N9 AIV emerged in February 2013 has caused 1,567 human cases,with a fatality rate of 39.2%(http://gffzz36b398b81f8a4a4ch5b9f6k9nv56x6u96.ffgz.tsg.suse.edu.cn/influenza/ | Chuansong Quan Ting Huang Xiuwei Chen Jie Zhang Qianli Wang Cheng Zhang Tiyan Zhang Lijun Zhou Liumei Shu Chunrong Long Lei Yang Xunbo Du Yingze Zhao Peipei Liu Hao Song Weifeng Shi Yuhai Bi Qiang Lv William J.Liu George F.Gao | 2018 | Science China(Life Sciences)2018，61，9： | 4 |
| 15 | A Neuronal Pathway that Commands Deceleration in Drosophila Larval Light-Avoidance显示文摘When facing a sudden danger or aversive condition while engaged in on-going forward motion,animals transiently slow down and make a turn to escape.The neural mechanisms underlying stimulation-induced deceleration in avoidance behavior are largely unknown.Here, we report that in Drosophila larvae, light-induced deceleration was commanded by a continuous neural pathway that included prothoracicotropic hormone neurons, eclosion hormone neurons, and tyrosine decarboxylase 2 motor neurons(the PET pathway). Inhibiting neurons in the PET pathway led to defects in lightavoidance due to insufficient deceleration and head casting.On the other hand, activation of PET pathway neurons specifically caused immediate deceleration in larval locomotion. Our findings reveal a neural substrate for the emergent deceleration response and provide a new understanding of the relationship between behavioral modules in animal avoidance responses. | Caixia Gong Zhenhuan Ouyang Weiqiao Zhao Jie Wang Kun Li Peipei Zhou Ting Zhao Nenggan Zheng Zhefeng Gong | 2019 | Neuroscience Bulletin2019，35，6： | 3 |
| 16 | Genomic characterization of SARS-CoV-2 identified in a reemerging COVID-19 outbreak in Beijing's Xinfadi market in 2020显示文摘After 56 days without coronavirus disease 2019(COVID-19)cases,reemergent cases were reported in Beijing,China on June 11,2020.Here,we report the genetic characteristics of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)sequenced from the clinical specimens of 4 human cases and 2 environmental samples.The nucleotide similarity among six SARS-CoV-2 genomes ranged from 99.98%to 99.99%.Compared with the reference strain of SARS-CoV-2(GenBank No.NC_045512),all six genome sequences shared the same substitutions at nt241(C→T),nt3037(C→T),nt14408(C→T),nt23403(A→G),nt28881(G→A),nt28882(G→A),and nt28883(G→C),which are the characteristic nucleotide substitutions of L-lineage European branch I.This was also proved by themaximum likelihood phylogenetic tree based on the full-length genome of SARS-CoV-2.They also have a unique shared nucleotide substitution,nt6026(C→T),which is the characteristic nucleotide substitution of SARS-CoV-2 in Beijing's Xinfadi outbreak.It is noteworthy that there is an amino acid D614Gmutation caused by nt23403 substitution in all six genomes,which may enhance the virus's infectivity in humans and help it become the leading strain of the virus to spread around the world today.It is necessary to continuously monitor the genetic variation of SARS-CoV-2,focusing on the influence of key mutation sites of SARS-CoV-2 on viral transmission,clinical manifestations,severity,and course of disease. | Yong Zhang Yang Pan Xiang Zhao Weifeng Shi Zhixiao Chen Sheng Zhang Peipei Liu Jinbo Xiao Wenjie Tan Dayan Wang William J.Liu Wenbo Xu Quanyi Wang Guizhen Wu | 2020 | Biosafety and Health2020，2，4： | 3 |
| 17 | Functional Near-Infrared Spectroscopy Neurofeedback of Cortical Target Enhances Hippocampal Activation and Memory Performance显示文摘Dear Editor,Neuromodulation,a rapidly expanding field attracting wide attention over recent decades,facilitates human cognition,behavior,and pathology by modifying the activity of specific neural targets.Human brain functions can be modified by exogenous brain neuromodulation techniques that deliver physical energy(e.g.,electrical current or magnetic pulses)into the brain[1],such as deep brain stimulation,transcranial magnetic stimulation,and tran-scranial direct current stimulation. | Xin Hou Xiang Xiao Yilong Gong Yihan Jiang Peipei Sun Juan Li Zheng Li Xiaojie Zhao Li Yao Antao Chen Chaozhe Zhu | 2021 | Neuroscience Bulletin2021，37，8： | 3 |
| 18 | Tracking SARS-CoV-2 Omicron diverse spike gene mutations identifies multiple inter-variant recombination events显示文摘The current pandemic of COVID-19 is fueled by more infectious emergent Omicron variants.Ongoing concerns of emergent variants include possible recombinants,as genome recombination is an important evolutionary mechanism for the emergence and re-emergence of human viral pathogens.In this study,we identified diverse recombination events between two Omicron major subvariants(BA.1 and BA.2)and other variants of concern(VOCs)and variants of interest(VOIs),suggesting that co-infection and subsequent genome recombination play important roles in the ongoing evolution of SARS-CoV-2.Through scanning high-quality completed Omicron spike gene sequences. | Junxian Ou Wendong Lan Xiaowei Wu Tie Zhao Biyan Duan Peipei Yang Yi Ren Lulu Quan Wei Zhao Donald Seto James Chodosh Zhen Luo Jianguo Wu Qiwei Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 3 |
| 19 | Extraction,Isolation and Structural Characterization of Polysaccharides from a Red Alga Gloiopeltis furcata显示文摘Three kinds of polysaccharides:GFW,GFH and GFA,were sequentially extracted from a red alga Gloiopeltis furcatawith 25℃and 85℃water,and 60℃ 4%NaOHwater solution.Based on the defatted alga,the yields of the polysaccharide were57.9%,2.5%and 2.6%,respectively.Their monosaccharide compositions,average molecular weights and structural characters weredetermined by gas chromatography(GC),high performance liquid chromatography(HPLC),fourier transform infrared spectroscopy(FTIR)or13C-NMR spectroscopy.The results showed that GFW,GFH and GFA were all composed of D-galactose(Gal)and3,6-anhydro-L-galactose(AnG),and particularly GFA also contained xylose(Xyl).The average molecular weights of GFW,GFHand GFA were 22.6 kD,26.5 kD and 49.8 kD,respectively,with the respective sulfate content 31.2%,25.1%and 22.7%.The data ofFTIR and13C-NMR confirmed the sulfate ester location at C6 of galactose.It is concluded that all the three polysaccharides extractedfrom Gloiopeltis furcata were sulfated galactans,two being sulfated-agarose,and one being xylose-containing sulfated galactan. | YU Guangli HU Yannan YANG Bo ZHAO Xia WANG Peipei JI Guoli WU Jiandong GUAN Huashi | 2010 | Journal of Ocean University of China2010，9，2： | 3 |
| 20 | Arenobufagin is a novel isoform-specific probe for sensing human sulfotransferase 2A1显示文摘Human cytosolic sulfotransferase 2 A1(SULT2A1) is an important phase II metabolic enzyme. The detection of SULT2A1 is helpful for the functional characterization of SULT2A1 and diagnosis of its related diseases. However, due to the overlapping substrate specificity among members of the sulfotransferase family, it is difficult to develop a probe substrate for selective detection of SULT2A1. In the present study, through characterization of the sulfation of series of bufadienolides, arenobufagin(AB) was proved as a potential probe substrate for SULT2A1 with high sensitivity and specificity. Subsequently, the sulfation of AB was characterized by experimental and molecular docking studies. The sulfate-conjugated metabolite was identified as AB-3-sulfate.The sulfation of AB displayed a high selectivity for SULT2A1 which was confirmed by in vitro reaction phenotyping assays. The sulfation of AB by human liver cytosols and recombinant SULT2A1 both obeyed Michaelis-Menten kinetics, with similar kinetic parameters. Molecular docking was performed to understand the interaction between AB and SULT2A1, in which the lack of interaction with Met-137 and Tyr-238 of SULT2A1 made it possible to eliminate substrate inhibition of AB sulfation. Finally, the probe was successfully used to determine the activity of SULT2A1 and its isoenzymes in tissue preparations of human and laboratory animals. | Xiangge Tian Chao Wang Peipei Dong Yue An Xinyu Zhao Weiru Jiang Gang Wang Jie Hou Lei Feng Yan Wang Guangbo Ge Xiaokui Huo Jing Ning Xiaochi Ma | 2018 | Acta Pharmaceutica Sinica B2018，8，5： | 2 |
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