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1TDD-net: a tiny defect detection network for printed circuit boards显示文摘Tiny defect detection (TDD) which aims to perform the quality control of printed circuit boards (PCBs) is a basic and essential task in the production of most electronic products. Though significant progress has been made in PCB defect detection, traditional methods are still difficult to cope with the complex and diverse PCBs. To deal with these problems, this article proposes a tiny defect detection network (TDD-Net) to improve performance for PCB defect detection. In this method, the inherent multi-scale and pyramidal hierarchies of deep convolutional networks are exploited to construct feature pyramids. Compared with existing approaches, the TDD-Net has three novel changes. First, reasonable anchors are designed by using k-means clustering. Second, TDD-Net strengthens the relationship of feature maps from different levels and benefits from low-level structural information, which is suitable for tiny defect detection. Finally, considering the small and imbalance dataset, online hard example mining is adopted in the whole training phase in order to improve the quality of region-of-interest (ROI) proposals and make more effective use of data information. Quantitative results on the PCB defect dataset show that the proposed method has better portability and can achieve 98.90% mAP, which outperforms the state-of-arts. The code will be publicly available.Runwei Ding Linhui Dai Guangpeng Li Hong Liu 2019CAAI Transactions on Intelligence Technology2019,4,2:27
2Simultaneous virus identification and characterization of severe unexplained pneumonia cases using a metagenomics sequencing technique显示文摘Many viruses can cause respiratory diseases in humans.Although great advances have been achieved in methods of diagnosis,it remains challenging to identify pathogens in unexplained pneumonia(UP) cases.In this study,we applied next-generation sequencing(NGS) technology and a metagenomic approach to detect and characterize respiratory viruses in UP cases from Guizhou Province,China.A total of 33 oropharyngeal swabs were obtained from hospitalized UP patients and subjected to NGS.An unbiased metagenomic analysis pipeline identified 13 virus species in 16 samples.Human rhinovirus C was the virus most frequently detected and was identified in seven samples.Human measles virus,adenovirus B 55 and coxsackievirus A10 were also identified.Metagenomic sequencing also provided virus genomic sequences,which enabled genotype characterization and phylogenetic analysis.For cases of multiple infection,metagenomic sequencing afforded information regarding the quantity of each virus in the sample,which could be used to evaluate each viruses' role in the disease.Our study highlights the potential of metagenomic sequencing for pathogen identification in UP cases.Xiaohui Zou Guangpeng Tang Xiang Zhao Yan Huang Tao Chen Mingyu Lei Wenbing Chen Lei Yang Wenfei Zhu Li Zhuang Jing Yang Zhaomin Feng Dayan Wang Dingming Wang Yuelong Shu 2017Science China(Life Sciences)2017,60,3:14
3Risk factors for pertussis among children hospitalized for pertussis during 2016-2017, in Guizhou Province of China: a case-control study显示文摘Aim:Pertussis is a respiratory tract infection,the vaccine for which was introduced in the Expanded Programme on Immunization(EPI)in Guizhou Province of China(hereafter referred as Guizhou)in the 1980s.This vaccine rapidly decreased incidence rates of pertussis in the province,however,despite the wide high coverage of the diphtheria,tetanus and acellular pertussis(DTaP)combined vaccine,there has been a resurgence of pertussis since 2014.Even with this recent increase in disease transmission,risk factors for pertussis infection have not been evaluated in Guizhou.We aimed to provide information on pertussis risk factors and insight on designing targeted pertussis control policies and measures through this study.Methods:A 1:2 matched retrospective case-control study was conducted between 2016 and 2017,involving infants and children younger than 6 years old and parents of the participants.The enrolled cases included clinical and laboratory confirmed pertussis cases according to the WHO-recommended pertussis definition.Controls were selected from children in the same neighborhood who were not diagnosed with pertussis prior to our investigation and did not exhibit any clinical manifestations of pertussis.Results:The household size[Matched odds ratio(OR_(m)=1.4,95%confidence interval(Cl):1.1-1.7]and household members with antecedent cough(OR_(m)=3.6,95%CI:1.8-7.2)were significantly associated with their child’s pertussis onset.The parents’occupations were significantly associated with their child’s pertussis onset(ORm=9.4,95%CI:1.6-54.8,for mother side;ORm=4.5,95%CI:1.2-16.5,for father side),when they worked in the business and/or service industry.Having family members with a history of cough was an independent risk factor for pertussis[Adjusted odds ratio(AOR)=43.6,95%CI:2.7-694.0].Besides,the parents’demographic characteristics and DTaP doses were not found to be independent factors.Conclusion:Household exposure is an important risk factor for pertussis infection in infants and young children and should therefore be considered as a major factor during formulation of pertussis control policies and measures.Feng Jiang Keli Li Guangpeng Tang Yan Huang 2021Global Health Journal2021,5,2:3
4Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda显示文摘A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.DING Bo SHI Peng XIANGYU Jinggong ZHANG Yaping CHEN Dayuan SUN Qingyuan LI Guangpeng WANG Minkang LIU Yilong KANG Li HAN Zhiming SONG Xiangfen LI Jingsong CHEN Yuchun 2000Chinese Science Bulletin2000,45,20:3
5Optimization of Fermentation Process of Carrot Juice by Probiotics and Analysis of Aroma Components显示文摘Carrot juice was fermented by probiotic Lactobacillus casei( CTCF-LC1) and Lactobacillus reuteri( CTCF-LT1) with sensory score as the main evaluation index,and the fermentation process was optimized by single factor and response surface experiments.The optimum ratio of fermentative strains CTCF-LC1∶ CTCF-LT1 was 1∶ 1,fermentation time was 8 h,inoculum size was 3.1% and fermentation temperature was26.6℃.Under the optimum conditions,the sensory score of fermented carrot juice was 91.3,pH was 4.50,total acid content was 3.73 g/L,total sugar content was 54.25 g/L,and reducing sugar content was 28.41 g/L.The carrot juice has bright orange color and stable state.It not only has the fresh flavor of fruit and vegetable juice,but also has the unique aroma from lactic acid bacteria fermentation,as well as soft and even taste,moderate acidity and sweetness.The changes of aroma components of carrot juice before and after fermentation were analyzed by headspace solid phase microextraction( HSPME) coupled with gas chromatography-mass spectrometry( GC-MS).After fermentation,substances with medicinal flavor components were reduced,and a lot of floral and fruit-scented substances were produced,such as methyl myristate,β-pinene,phenylethanol,and geraniol.Therefore,the results of this study can provide a theoretical basis for the development of fermented fruit and vegetable juice products with unique flavor substances.Xiaotian LI Guangpeng LIU Jinglong LI Fengtao ZHU Bo ZHAO Yan ZHAO Fatao HE 2019Asian Agricultural Research2019,11,1:2
6Comparative Study on Functional Properties of Mulberry Black Tea Fermented by Phellinus igniarius and Eurotium cristatum显示文摘[Objectives]To compare functional properties of mulberry black tea fermented by Phellinus igniarius and Eurotium cristatum.[Methods]The functional components of mulberry black tea fermented by P.igniarius and E.cristatum,the deep processing and health care effects of two probiotic fungi were explored.[Results]The polysaccharide content in the tea fermented by P.igniarius was the highest at 0.410 mg/mL,and the hydroxyl radical scavenging rate was the strongest,reaching 60.31%;the content of flavonoids in the tea fermented by E.cristatum was the highest,reaching 60.65μg/mL,and the content of polysaccharides was 83.17%higher than that of mulberry black tea,reaching 0.279 mg/mL.[Conclusions]The mulberry black tea fermented by P.igniarius and E.cristatum has improved in different functional components,and has its own advantages in aroma and mouthfeel,so it has certain research value and market prospect.Yanrui MA Xuezhen LI Xuemei YANG Guangpeng LIU Le CHU Yan ZHAO Fatao HE 2022Medicinal Plant2022,13,3:2
7Towards Practical Application of Li-S Battery with High Sulfur Loading and Lean Electrolyte:Will Carbon-Based Hosts Win This Race?显示文摘As the need for high-energy–density batteries continues to grow, lithium-sulfur(Li–S) batteries have become a highly promising next-generation energy solution due to their low cost and exceptional energy density compared to commercially available Li-ion batteries. Research into carbon-based sulfur hosts for Li–S batteries has been ongoing for over two decades, leading to a significant number of publications and patents.However, the commercialization of Li–S batteries has yet to be realized. This can be attributed, in part, to the instability of the Li metal anode. However, even when considering just the cathode side, there is still no consensus on whether carbon-based hosts will prove to be the best sulfur hosts for the industrialization of Li–S batteries. Recently, there has been controversy surrounding the use of carbon-based materials as the ideal sulfur hosts for practical applications of Li–S batteries under high sulfur loading and lean electrolyte conditions. To address this question, it is important to review the results of research into carbon-based hosts, assess their strengths and weaknesses, and provide a clear perspective. This review systematically evaluates the merits and mechanisms of various strategies for developing carbon-based host materials for high sulfur loading and lean electrolyte conditions. The review covers structural design and functional optimization strategies in detail, providing a comprehensive understanding of the development of sulfur hosts. The review also describes the use of efficient machine learning methods for investigating Li–S batteries. Finally, the outlook section lists and discusses current trends, challenges, and uncertainties surrounding carbon-based hosts, and concludes by presenting our standpoint and perspective on the subject.Yi Gong Jing Li Kai Yang Shaoyin Li Ming Xu Guangpeng Zhang Yan Shi Qiong Cai Huanxin Li Yunlong Zhao 2023Nano-Micro Letters2023,15,9:1
8Matrix attachment regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgenic cells and transgenic mice显示文摘In the present study, follistatin(FST) gene expression vectors with either a bicistronic gene transfer cassette alone, or a bicistron gene cassette carrying a matrix attachment region(MAR) were constructed and transfected to bovine fetal fibroblasts. Evaluations of both the integration and expression of exogenous FST indicated that the p MAR-CAG-FST-IRES-Ac GFP1-poly A-MAR(pMAR-FST) vector had higher capacity to form monoclonal transgenic cells than the vector without MAR,though transient transfection and integration efficiency were similar with either construct. Remarkably, protein expression in transgenic cells with the p MAR-FST vector was significantly higher than that from the bicistronic vector. Exogenous FST was expressed in all of the p MARFST transgenic mice at F_0, F_1 and F_2. Total muscle growth in F_0 mice was significantly greater than in wild-type mice,with larger muscles in fore and hind limbs of transgenic mice. p MAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner spaces between sarcolemma, which suggests a correlation between transgene expression-associated muscle development and the trend of muscle growth. In conclusion, a p MAR-FST vector, which excluded the resistant genes and frame structure, enhances and stabilizes FST gene expressions in both transfected cells and transgenic mice.Xiaoming HU Jing GUO Chunling BAI Zhuying WEI Li GAO Tingmao HU Shorgan BOU Guangpeng LI 2016Frontiers of Agricultural Science and Engineering2016,3,1:1
9Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core显示文摘Guangpeng Ma Youjun Feng Feng Gao Jinzi Wang Cheng Liu Yijing Li 2005Biochemical and Biophysical Research Communications2005,,4:1
10In vivo and in vitro development of Tibetan antelope(Pantholops hodgsonii)interspecific cloned embryos显示文摘The Tibetan antelope is endemic to the Tibetan Plateau,China,and is now considered an endangered species.As a possible rescue strategy,the development of embryos constructed by interspecies somatic cell nuclear transfer(iSCNT)was examined.Tibetan antelope fibroblast cells were transferred into enucleated bovine,ovine and caprine oocytes.These cloned embryos were then cultured in vitro or in the oviducts of intermediate animals.Less than 0.5%of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst stage.However,when the cloned antelope-bovine embryos were transferred to caprine oviducts,about 1.6%of the embryos developed to the blastocyst stage.In contrast,only 0.7%of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine oviducts.The treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and caprine.When the antelope-bovine embryos,constructed from oocytes treated with roscovitine or trichostatin A,were cultured in rabbit oviducts 2.3%and 14.3%developed to blastocysts,respectively.It is concluded that although some success was achieved with the protocols used,interspecies cloning of Tibetan antelope presents difficulties still to be overcome.The mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.Guanghua SU Lei CHENG Yu GAO Kun LIU Zhuying WEI Chunling BAI Fengxia YIN Li GAO Guangpeng LI Shorgan BOU 2014Frontiers of Agricultural Science and Engineering2014,1,1:1
11Feasibility and physics potential of detecting ^(8)B solar neutrinos at JUNO显示文摘The Jiangmen Underground Neutrino Observatory(JUNO)features a 20 kt multi-purpose underground liquid scintillator sphere as its main detector.Some of JUNO's features make it an excellent location for^8B solar neutrino measurements,such as its low-energy threshold,high energy resolution compared with water Cherenkov detectors,and much larger target mass compared with previous liquid scintillator detectors.In this paper,we present a comprehensive assessment of JUNO's potential for detecting^8B solar neutrinos via the neutrino-electron elastic scattering process.A reduced 2 MeV threshold for the recoil electron energy is found to be achievable,assuming that the intrinsic radioactive background^(238)U and^(232)Th in the liquid scintillator can be controlled to 10^(-17)g/g.With ten years of data acquisition,approximately 60,000 signal and 30,000 background events are expected.This large sample will enable an examination of the distortion of the recoil electron spectrum that is dominated by the neutrino flavor transformation in the dense solar matter,which will shed new light on the inconsistency between the measured electron spectra and the predictions of the standard three-flavor neutrino oscillation framework.IfDelta m^(2)_(21)=4.8times10^(-5);(7.5times10^(-5))eV^(2),JUNO can provide evidence of neutrino oscillation in the Earth at approximately the 3sigma(2sigma)level by measuring the non-zero signal rate variation with respect to the solar zenith angle.Moreover,JUNO can simultaneously measureDelta m^2_(21)using^8B solar neutrinos to a precision of 20% or better,depending on the central value,and to sub-percent precision using reactor antineutrinos.A comparison of these two measurements from the same detector will help understand the current mild inconsistency between the value of Delta m^2_(21)reported by solar neutrino experiments and the KamLAND experiment.Angel Abusleme Thomas Adam Shakeel Ahmad Sebastiano Aiello Muhammad Akram Nawab Ali Fengpeng An Guangpeng An Qi An Giuseppe Andronico Nikolay Anfimov Vito Antonelli Tatiana Antoshkina Burin Asavapibhop João Pedro Athayde Marcondes de André Didier Auguste Andrej Babic Wander Baldini Andrea Barresi Eric Baussan Marco Bellato Antonio Bergnoli Enrico Bernieri David Biare Thilo Birkenfeld Sylvie Blin David Blum Simon Blyth Anastasia Bolshakova Mathieu Bongrand Clément Bordereau Dominique Breton Augusto Brigatti Riccardo Brugnera Riccardo Bruno Antonio Budano Max Buesken Mario Buscemi Jose Busto Ilya Butorov Anatael Cabrera Hao Cai Xiao Cai Yanke Cai Zhiyan Cai Antonio Cammi Agustin Campeny Chuanya Cao Guofu Cao Jun Cao Rossella Caruso Cédric Cerna Jinfan Chang Yun Chang Pingping Chen Po-An Chen Shaomin Chen Shenjian Chen Xurong Chen Yi-Wen Chen Yixue Chen Yu Chen Zhang Chen Jie Cheng Yaping Cheng Alexander Chepurnov Davide Chiesa Pietro Chimenti Artem Chukanov Anna Chuvashova Gérard Claverie Catia Clementi Barbara Clerbaux Selma Conforti Di Lorenzo Daniele Corti Salvatore Costa Flavio Dal Corso Christophe De La Taille Jiawei Deng Zhi Deng Ziyan Deng Wilfried Depnering Marco Diaz Xuefeng Ding Yayun Ding Bayu Dirgantara Sergey Dmitrievsky Tadeas Dohnal Georgy Donchenko Jianmeng Dong Damien Dornic Evgeny Doroshkevich Marcos Dracos Frédéric Druillole Shuxian Du Stefano Dusini Martin Dvorak Timo Enqvist Heike Enzmann Andrea Fabbri Lukas Fajt Donghua Fan Lei Fan Can Fang Jian Fang Marco Fargetta Anna Fatkina Dmitry Fedoseev Vladko Fekete Li-Cheng Feng Qichun Feng Richard Ford Andrey Formozov Amélie Fournier Haonan Gan Feng Gao Alberto Garfagnini Alexandre Göttel Christoph Genster Marco Giammarchi Agnese Giaz Nunzio Giudice Franco Giuliani Maxim Gonchar Guanghua Gong Hui Gong Oleg Gorchakov Yuri Gornushkin Marco Grassi Christian Grewing Maxim Gromov Vasily Gromov Minghao Gu Xiaofei Gu Yu Gu Mengyun Guan Nunzio Guardone Maria Gul Cong Guo Jingyuan Guo Wanlei Guo Xinheng Guo Yuhang Guo Paul Hackspacher Caren Hagner Ran Han Yang Han Miao He Wei He Tobias Heinz Patrick Hellmuth Yuekun Heng Rafael Herrera Daojin Hong YuenKeung Hor Shaojing Hou Yee Hsiung Bei-Zhen Hu Hang Hu Jianrun Hu Jun Hu Shouyang Hu Tao Hu Zhuojun Hu Chunhao Huang Guihong Huang Hanxiong Huang Qinhua Huang Wenhao Huang Xingtao Huang Yongbo Huang Jiaqi Hui Wenju Huo Cédric Huss Safeer Hussain Antonio Insolia Ara Ioannisian Daniel Ioannisyan Roberto Isocrate Kuo-Lun Jen Xiaolu Ji Xingzhao Ji Huihui Jia Junji Jia Siyu Jian Di Jiang Xiaoshan Jiang Ruyi Jin Xiaoping Jing Cécile Jollet Jari Joutsenvaara Sirichok Jungthawan Leonidas Kalousis Philipp Kampmann Li Kang Michael Karagounis Narine Kazarian Amir Khan Waseem Khan Khanchai Khosonthongkee Patrick Kinz Denis Korablev Konstantin Kouzakov Alexey Krasnoperov Svetlana Krokhaleva Zinovy Krumshteyn Andre Kruth Nikolay Kutovskiy Pasi Kuusiniemi Tobias Lachenmaier Cecilia Landini Sébastien Leblanc Frederic Lefevre Liping Lei Ruiting Lei Rupert Leitner Jason Leung Demin Li Fei Li Fule Li Haitao Li Huiling Li Jiaqi Li Jin Li Kaijie Li Mengzhao Li Nan Li Nan Li Qingjiang Li Ruhui Li Shanfeng Li Shuaijie Li Tao Li Weidong Li Weiguo Li Xiaomei Li Xiaonan Li Xinglong Li Yi Li Yufeng Li Zhibing Li Ziyuan Li Hao Liang Hao Liang Jingjing Liang Jiajun Liao Daniel Liebau Ayut Limphirat Sukit Limpijumnong Guey-Lin Lin Shengxin Lin Tao Lin Jiajie Ling Ivano Lippi Fang Liu Haidong Liu Hongbang Liu Hongjuan Liu Hongtao Liu Hu Liu Hui Liu Jianglai Liu Jinchang Liu Min Liu Qian Liu Qin Liu Runxuan Liu Shuangyu Liu Shubin Liu Shulin Liu Xiaowei Liu Yan Liu Alexey Lokhov Paolo Lombardi Claudio Lombardo Kai Loo Chuan Lu Haoqi Lu Jingbin Lu Junguang Lu Shuxiang Lu Xiaoxu Lu Bayarto Lubsandorzhiev Sultim Lubsandorzhiev Livia Ludhova Fengjiao Luo Guang Luo Pengwei Luo Shu Luo Wuming Luo Vladimir Lyashuk Qiumei Ma Si Ma Xiaoyan Ma Xubo Ma Jihane Maalmi Yury Malyshkin Fabio Mantovani Francesco Manzali Xin Mao Yajun Mao Stefano MMari Filippo Marini Sadia Marium Cristina Martellini Gisele Martin-Chassard Agnese Martini Davit Mayilyan Axel Müller Ints Mednieks Yue Meng Anselmo Meregaglia Emanuela Meroni David Meyhöfer Mauro Mezzetto Jonathan Miller Lino Miramonti Salvatore Monforte Paolo Montini Michele Montuschi Nikolay Morozov Pavithra Muralidharan Massimiliano Nastasi Dmitry VNaumov Elena Naumova Igor Nemchenok Alexey Nikolaev Feipeng Ning Zhe Ning Hiroshi Nunokawa Lothar Oberauer Juan Pedro Ochoa-Ricoux Alexander Olshevskiy Domizia Orestano Fausto Ortica Hsiao-Ru Pan Alessandro Paoloni Nina Parkalian Sergio Parmeggiano Teerapat Payupol Yatian Pei Nicomede Pelliccia Anguo Peng Haiping Peng Frédéric Perrot Pierre-Alexandre Petitjean Fabrizio Petrucci Luis Felipe Piñeres Rico Oliver Pilarczyk Artyom Popov Pascal Poussot Wathan Pratumwan Ezio Previtali Fazhi Qi Ming Qi Sen Qian Xiaohui Qian Hao Qiao Zhonghua Qin Shoukang Qiu Muhammad Rajput Gioacchino Ranucci Neill Raper Alessandra Re Henning Rebber Abdel Rebii Bin Ren Jie Ren Taras Rezinko Barbara Ricci Markus Robens Mathieu Roche Narongkiat Rodphai Aldo Romani Bedřich Roskovec Christian Roth Xiangdong Ruan Xichao Ruan Saroj Rujirawat Arseniy Rybnikov Andrey Sadovsky Paolo Saggese Giuseppe Salamanna Simone Sanfilippo Anut Sangka Nuanwan Sanguansak Utane Sawangwit Julia Sawatzki Fatma Sawy Michaela Schever Jacky Schuler Cédric Schwab Konstantin Schweizer Dmitry Selivanov Alexandr Selyunin Andrea Serafini Giulio Settanta Mariangela Settimo Muhammad Shahzad Vladislav Sharov Gang Shi Jingyan Shi Yongjiu Shi Vitaly Shutov Andrey Sidorenkov FedorŠimkovic Chiara Sirignano Jaruchit Siripak Monica Sisti Maciej Slupecki Mikhail Smirnov Oleg Smirnov Thiago Sogo-Bezerra Julanan Songwadhana Boonrucksar Soonthornthum Albert Sotnikov Ondrej Sramek Warintorn Sreethawong Achim Stahl Luca Stanco Konstantin Stankevich DušanŠtefánik Hans Steiger Jochen Steinmann Tobias Sterr Matthias Raphael Stock Virginia Strati Alexander Studenikin Gongxing Sun Shifeng Sun Xilei Sun Yongjie Sun Yongzhao Sun Narumon Suwonjandee Michal Szelezniak Jian Tang Qiang Tang Quan Tang Xiao Tang Alexander Tietzsch Igor Tkachev Tomas Tmej Konstantin Treskov Andrea Triossi Giancarlo Troni Wladyslaw Trzaska Cristina Tuve Stefan van Waasen Johannes van den Boom Guillaume Vanroyen Nikolaos Vassilopoulos Vadim Vedin Giuseppe Verde Maxim Vialkov Benoit Viaud Cristina Volpe Vit Vorobel Lucia Votano Pablo Walker Caishen Wang Chung-Hsiang Wang En Wang Guoli Wang Jian Wang Jun Wang Kunyu Wang Lu Wang Meifen Wang Meng Wang Ruiguang Wang Siguang Wang Wei Wang Wenshuai Wang Xi Wang Xiangyue Wang Yangfu Wang Yaoguang Wang Yi Wang Yifang Wang Yuanqing Wang Yuman Wang Zhe Wang Zheng Wang Zhimin Wang Zongyi Wang Apimook Watcharangkool Lianghong Wei Wei Wei Yadong Wei Liangjian Wen Christopher Wiebusch Steven Chan-Fai Wong Bjoern Wonsak Diru Wu Fangliang Wu Qun Wu Wenjie Wu Zhi Wu Michael Wurm Jacques Wurtz Christian Wysotzki Yufei Xi Dongmei Xia Yuguang Xie Zhangquan Xie Zhizhong Xing Benda Xu Donglian Xu Fanrong Xu Jilei Xu Jing Xu Meihang Xu Yin Xu Yu Xu Baojun Yan Xiongbo Yan Yupeng Yan Anbo Yang Changgen Yang Huan Yang Jie Yang Lei Yang Xiaoyu Yang Yifan Yang Haifeng Yao Zafar Yasin Jiaxuan Ye Mei Ye Ugur Yegin Frédéric Yermia Peihuai Yi Xiangwei Yin Zhengyun You Boxiang Yu Chiye Yu Chunxu Yu Hongzhao Yu Miao Yu Xianghui Yu Zeyuan Yu Chengzhuo Yuan Ying Yuan Zhenxiong Yuan Ziyi Yuan Baobiao Yue Noman Zafar Andre Zambanini Pan Zeng Shan Zeng Tingxuan Zeng Yuda Zeng Liang Zhan Feiyang Zhang Guoqing Zhang Haiqiong Zhang Honghao Zhang Jiawen Zhang Jie Zhang Jingbo Zhang Peng Zhang Qingmin Zhang Shiqi Zhang Tao Zhang Xiaomei Zhang Xuantong Zhang Yan Zhang Yinhong Zhang Yiyu Zhang Yongpeng Zhang Yuanyuan Zhang Yumei Zhang Zhenyu Zhang Zhijian Zhang Fengyi Zhao Jie Zhao Rong Zhao Shujun Zhao Tianchi Zhao Dongqin Zheng Hua Zheng Minshan Zheng Yangheng Zheng Weirong Zhong Jing Zhou Li Zhou Nan Zhou Shun Zhou Xiang Zhou Jiang Zhu Kejun Zhu Honglin Zhuang Liang Zong Jiaheng Zou 2021Chinese Physics C2021,45,2:0
12Screening and Properties of Functional Probiotics for Apple Fermentation显示文摘[Objectives]To screen functional probiotics for apple fermentation and study their probiotic properties.[Methods]A total of 35 strains were screened from traditional fermented foods in different regions.After primary screening,re-screening and research on growth characteristics,two characteristic strains,CTCF-LC1(LC1,with strong acid production ability)and CTCF-LT1(LT1,with strong adaptability),were obtained.[Results]The results of physiological and biochemical identification and 16S DNA-based identification show that LC1 is Lactobacillus casei and LT1 is Lactobacillus reuteri.Their fermentation performance in apple juice was tested,and it was found that the two strains grew well in the fermentation broth,and the total number of colonies could reach 10^(9) cfu/mL.The final pH value of LC1 fermentation broth dropped below 3.5,and the total acid content exceeded 12 g/L.The pH of LT1 fermentation broth dropped to 3.9,and the total acid content also exceeded 6 g/L.In the study on the antioxidation property of apple juice,it was found that after high temperature and high pressure steam sterilization,the DPPH free radical scavenging rate of apple juice extract increased by 35.8%,and potassium ferricyanide reduction capability increased by 68%.After fermentation by LC1 and LT1,the potassium ferricyanide reducing power of apple juice was increased by 42.9%.[Conclusions]This has a good application prospect in the development of fruit and vegetable juice fermented beverages.Yanrui MA Xiaotian LI Fatao HE Guangpeng LIU Fengtao ZHU Guifang QIN Le CHU Jiwu WANG 2022Asian Agricultural Research2022,14,3:0
13Prediction of forest fire occurrence in China under climate change scenarios显示文摘Climate change has an impact on forest fire patterns.In the context of global warming,it is important to study the possible effects of climate change on forest fires,carbon emission reductions,carbon sink effects,forest fire management,and sustainable development of forest ecosystems.This study is based on MODIS active fire data from 2001-2020 and the influence of climate,topography,vegetation,and social factors were integrated.Temperature and precipitation information from different scenarios of the BCC-CSM2-MR climate model were used as future climate data.Under climate change scenarios of a sustainable low development path and a high conventional development path,the extreme gradient boosting model predicted the spatial distribution of forest fire occurrence in China in the 2030s(2021-2040),2050s(2041-2060),2070s(2061-2080),and2090s(2081-2100).Probability maps were generated and tested using ROC curves.The results show that:(1)the area under the ROC curve of training data(70%)and validation data(30%)were 0.8465 and 0.8171,respectively,indicating that the model can reasonably predict the occurrence of forest fire in the study area;(2)temperature,elevation,and precipitation were strongly correlated with fire occurrence,while land type,slope,distance from settlements and roads,and slope direction were less strongly correlated;and,(3)based on future climate change scenarios,the probability of forest fire occurrence will tend to shift from the south to the center of the country.Compared with the current climate(2001-2020),the occurrence of forest fires in 2021-2040,2041-2060,2061-2080,and 2081-2100 will increase significantly in Henan Province(Luoyang,Nanyang,S anmenxia),Shaanxi Province(Shangluo,Ankang),Sichuan Province(Mianyang,Guangyuan,Ganzi),Tibet Autonomous Region(Shannan,Linzhi,Changdu),Liaoning Province(Liaoyang,Fushun,Dandong).Yakui Shao Guangpeng Fan Zhongke Feng Linhao Sun Xuanhan Yang Tiantian Ma XuSheng Li Hening Fu Aiai Wang 2023Journal of Forestry Research2023,34,5:0
14Effects of Different Sterilization Conditions on Active Components and Flavor of Apple Vinegar显示文摘[Objectives]To explore the effects of different sterilization conditions on nutrition and flavor of apple vinegar.[Methods]Five kinds of high temperature short time(HTST)sterilization conditions were selected to treat apple vinegar,and the volatile aroma components and the content of active components in apple vinegar before and after sterilization were analyzed.[Results]Compared with the control,the contents of total acid and malic acid in the samples after sterilization changed little,but the contents of citric acid increased significantly(P<0.01),and the contents of total phenols,ascorbic acid and total flavonoids decreased.Ethyl acetate,isopentyl acetate,ethyl caprylate,phenethyl acetate,1-pentanol,phenylethyl alcohol,acetic acid,and sec-butyl ether were the characteristic aroma components which contributed to the flavor of apple vinegar.As sterilization temperature increased,the content of esters decreased,while the content of acids,alcohols and aldehydes increased.The contents of nutrition,active components and volatile aroma components in apple vinegar under 100℃and 30 s sterilization conditions had little change compared with other sterilization conditions,so 100℃and 30 s were the optimal sterilization conditions.[Conclusions]Under different sterilization conditions,the content of flavor components in apple vinegar will change greatly,which will affect the quality of apple vinegar.Yanrui MA Xuezhen LI Yongbo DOU Yuan MENG Yan ZHAO Gen LI Yanlin DONG Guangpeng LIU Le CHU Fengtao ZHU 2023Asian Agricultural Research2023,15,12:0
15Effect of Magnetic Field Technology on Preservation Quality of Fresh-cut Apple显示文摘[Objectives]The paper was to study the effect of magnetic field technology on physicochemical properties of fresh-cut apple during storage.[Methods]Using fresh-cut apples as materials,magnetic field assisted refrigeration and conventional refrigeration control groups were set up,and the preservation effects of magnetic field assisted refrigeration on fresh-cut apples were tested by measuring the changes of microorganisms and physicochemical indicators.In this experiment,the conditions of magnetic field assisted refrigeration group was set as follows:temperature 4℃,magnetic field intensity 5 mT,static magnetic field,and storage for 6 d.[Results]At 6 d post storage,the weight loss rate,total number of colonies and total number of fungi of fresh-cut apples increased;the L value and hardness showed a downward trend;the soluble solid content first increased and then decreased;and the total acid decreased first and then increased.The above indicators in magnetic field assisted refrigeration group changed more gently than that in conventional refrigeration group.Compared with conventional assisted refrigeration,the weight loss rate of fresh-cut apples treated by magnetic field combined with 4℃refrigeration was reduced by 68.00%;the hardness was increased by 12.23%;the total number of colonies and total number of fungi were reduced by 64.15%and 37.50%;and the L value did not change much.High content of soluble solids and total acid was maintained.[Conclusions]Magnetic field assisted refrigeration delays quality deterioration of fresh-cut apples during storage,prolongs shelf life,and further improves their edible quality and commodity value.Le CHU Jie SHEN Gen LI Ye SONG Yinfei MA Guangpeng LIU Maoyu WU Fatao HE Yan ZHAO 2023Asian Agricultural Research2023,15,3:0
16Pre‑rainy Season Rainstorms in South China—Risk Perception of the 11 April 2019 Rainstorm in Shenzhen City显示文摘With the acceleration of urbanization in South China, rainstorms and floods are threatening the safety of people in urban areas. The 11 April 2019(4·11 hereafter)rainstorm in Shenzhen City was a typical pre-rainy season rainstorm that caused great damage, yet such pre-rainy season events have not attracted sufficient attention in research.Risk perception of the public may indirectly affect their disaster preparedness, which is important for disaster management. In this study, we conducted a questionnaire survey that considered demographic factors and the level of risk perception, knowledge of risk, impact of the 4·11 rainstorm event on public risk perception, and degree of trust in the government. We used a two-factor model of risk perception to evaluate the factors that influenced public risk perception of the 4·11 rainstorm in Shenzhen. The main conclusions are: The 4·11 rainstorm improved public awareness of both risk and impact through the medium term, but the public’s perceived low probability of disaster occurrence and lack of knowledge of the pre-rainy season rainstorm phenomenon led to serious losses during this event. Although the public has high trust in the Shenzhen government, the management of rainstorm disasters in the pre-rainy season needs to be further improved.Xuran Sun Wei Zhou Guoming Zhang Lianyou Liu Guangpeng Wang Mingzhu Xiang Yuting Xiao Shufeng Qu Shouwei Li Jiaxue Li 2022International Journal of Disaster Risk Science2022,13,6:0
17Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2020显示文摘In 2020, the MOE Key Laboratory of Macromolecular Synthesis and Functionalization in Zhejiang University had made progresses in several aspects. First, a series of metal-free organoboron catalysts had been designed and synthesized facilely, exhibiting outstanding reactivity, thermalstability and productivity in different kinds of polymerization and cycloaddition reactions. Second, a variety of chalcogen(O, S, Se)-rich polymers had been synthesized via organocatalysis and fabricated to be the ionic conductive and photoluminescent materials. Third, diverse microenvironment-sensitive nanoparticles had been designed,and novel strategies had been realized, to enhance the therapeutic efficacy in cancer as well as biofilmassociated infections. Fourth, m6 A modification on cellular transcriptome-wide messenger RNA had been successfully mapped at single base resolution using a metabolic labeling method. Fifth, a hydrogel-based robot had been developed, showing swift locomotion as a response to dynamic light stimulations. Sixth,the conformation-size scaling law and the conformation evolution map of 2 D macromolecules in solution had been elucidated experimentally, in the single-layer graphene oxide model. Seventh, semitransparent polymer solar cells, promising as building-integrated photovoltaics, have been developed with the fine balance among power conversion efficiency, visible light transparency and infrared photon radiation rejection. Finally, long-range ordered bulk-heterojunctions of organic semiconductors had been achieved,and their superior optoelectronic properties and potential application in photoelectric conversion had been revealed. The related work progresses are reviewed in this paper.Jie Ren Xiao Shu Ya Wang Di Wang Guangpeng Wu Xinghong Zhang Qiao Jin Jianzhao Liu Ziliang Wu Zhen Xu Chang-Zhi Li Hanying Li 2022Chinese Chemical Letters2022,33,4:0
18Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2022显示文摘In 2022,The MOE Key Laboratory of Macromolecular Synthesis and Functionalization in Zhejiang University had achieved several important results.First,a series of well-defined dinuclear organoboron catalysts were developed to precisely control the enchainment of ether and carbonate segments during the copolymerization of CO_(2)and epoxides.Second,polyester had been synthesized through cationic copolymerization of cyclic anhydride.Third,ring-opening polymerization of carbon dioxide based valerolactone had been achieved,revealing the prospect of 3-ethylidene-6-vinyltetrahydro-2H-pyran-2-one(EVL)in utilizing CO_(2)and synthesizing functional polymers.Fourth,machine learning methods have been applied to biomaterial research,enabling high-throughput screening of functional biomaterial surfaces for implantable devices,and searching for potent antimicrobial peptides in whole combinatorial peptide libraries.Fifth,methods of characterization of biomacromolecule RNA transcription and manipulation of nucleoside modification were developed.Sixth,artificial enzymes-armed Bifidobacterium Longum probiotics were established to tune down gut inflammation.Seventh,three-dimensional(3D)printing technologies were used to engineer tough supramolecular hydrogels.Eighth,hydroplastic foaming graphene frameworks for acoustic and conductive polymer composites were provided for application.Ninth,aggregate photophysics about the nature of through-space interactions(TSIs)and manipulating their strength in small molecules with non-conjugated structure had been elucidated.Tenth,the forming mechanism of a newfound nested texture in poly(L-lactic acid)(PLLA)spherulitic films had been revealed.Finally,the isotropically dyeing mechanism of KDP single crystals grown from hydrogels have been explored.The related works are reviewed in this paper.Xumeng Deng Kaihao Chen Kai Pang Xiaoting Liu Minsong Gao Jie Ren Guanwen Yang Guangpeng Wu Chengjian Zhang Xufeng Ni Peng Zhang Jian Ji Jianzhao Liu Zhengwei Mao Ziliang Wu Zhen Xu Haoke Zhang Hanying Li 2024Chinese Chemical Letters2024,35,3:0
19The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,China显示文摘Since the birth of the first cloned sheep,somatic cell nuclear transfer technology has been successfully used to clone a variety of mammals.Cloned livestock have no apparent health risks,and the quality and safety of the cloned animal products are similar to non-cloned animals.The social behavior and environmental adaptability of postnatal cloned animals,especially when used for grassland farm production purposes,is unknown.In the present study,the cloned Dorper sheep equipped with GPS location devices were free-grazed in a harsh natural environment similar to conditions commonly experienced by Mongolian sheep.The main findings of this research were as follows.(1)Under free-grazing conditions,the cloned sheep showed excellent climatic and ecological adaptability.In extreme temperature conditions ranging from–30 to 40°C,the cloned sheep maintained acceptable body condition and behaved as other sheep.(2)The cloned sheep quickly adapted from a herd feeding strategy to the harsh environment and quickly exhibited a grazing regimen as other free-grazing sheep.(3)The cloned sheep exhibited free-grazing patterns and social behavior as other sheep.(4)The cloned sheep in the harsh environment thrived and produced healthy lambs.Overall,the cloned Dorper sheep exhibited excellent ecological adaptation,which is an important consideration for breeding meat sheep by cloning.The Dorper sheep readily adapted to the free-grazing conditions on the Mongolian plateau grassland,which attests to their ability to withstand harsh environmental conditions.Xinxin LI Huijuan WANG Guanghua SU Zhuying WEI Chunling BAI Wuni-MENGHE Yanhui HOU Changqing YU Shorgan BOU Guangpeng LI 2014Frontiers of Agricultural Science and Engineering2014,1,3:0
20Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer:a review显示文摘Oocytes are unique cells with the inherent capability to reprogram nuclei.The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer.The nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear reprogramming.The chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm chromatin.The oocyte first obtains its reprogramming capability in the early fetal follicle,and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle oocytes.The cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular reprogramming.Certain transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem cells.The maternal pronucleus exerts a predominant,transcriptiondependent effect on embryo cytofragmentation,with a lesser effect imposed by the ooplasm and the paternal pronucleus.With deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.Fengxia YIN Hui LIU Shorgan BOU Guangpeng LI 2014Frontiers of Agricultural Science and Engineering2014,1,2:0
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