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| 1 | A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs显示文摘African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig industry.An efficacious vaccine is urgently required.Here,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted viruses.The virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV challenge.Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV. | Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu | 2020 | Science China(Life Sciences)2020，63，5： | 72 |
| 2 | Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020显示文摘African swine fever virus(ASFV)has been circulating in China for more than two years,and it is not clear whether the biological properties of the virus have changed.Here,we report on our surveillance of ASFVs in seven provinces of China,from June to December,2020.A total of 22 viruses were isolated and characterized as genotype II ASFVs,with mutations,deletions,insertions,or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018(HLJ/18),the earliest isolate in China.Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a nonhemadsorbing(non-HAD)phenotype.Four isolates were tested for virulence in pigs;two were found to be as highly lethal as HLJ/18.However,two non-HAD isolates showed lower virulence but were highly transmissible;infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease,whereas 103 TCID50 dose caused non-lethal,sub-acute or chronic disease,and persistent infection.The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control. | Encheng Sun Zhenjiang Zhang Zilong Wang Xijun He Xianfeng Zhang Lulu Wang Wenqing Wang Lianyu Huang Fei Xi Haoyue Huangfu Ghebremedhin Tsegay Hong Huo Jianhong Sun Zhijun Tian Wei Xia Xuewu Yu Fang Li Renqiang Liu Yuntao Guan Dongming Zhao Zhigao Bu | 2021 | Science China(Life Sciences)2021，64，5： | 46 |
| 3 | mm Wave communications for 5G: implementation challenges and advances显示文摘The requirement of the fifth generation(5 G) wireless communication for high throughput motivates the wireless industry to use the mm Wave(millimeter wave) communications for its wide bandwidth advantage. To compensate the heavy path loss and increase the communications capacity, phased array beamforming and massive multiple-input multiple-output(MIMO) techniques are employed at both the user equipment(UE) and base stations(BS). Considering the commercial requirements, 5 G mm Wave large array systems should be implemented in an energy-and cost-efficient way with a small form factor. To address above issues and realize a reliable communications link, taking into account the particular characteristics of5 G mm Wave systems, this paper firstly examines the design challenges and trade-offs in system implementations, then some of the design strategies are summarized. At last, recent advance in RF front-end circuits and receiver sub-systems is then highlighted. | Lianming LI Dongming WANG Xiaokang NIU Yuan CHAI Linhui CHEN Long HE Xu WU Fuchun ZHENG Tiejun CUI Xiaohu YOU | 2018 | Science China(Information Sciences)2018，61，2： | 19 |
| 4 | Pore structure characterization and seepage analysis of ionic rare earth orebodies based on computed tomography images显示文摘Pore network structure of ore body is a diffusion channel of leaching agent solution that exerts a significant influence on seepage.The ore body structure,pore distribution,pore and throat size,and pore network characteristics of topsoil,weathered,and semiweathered layers of ionic rare earth ore in southern Jiangxi Province were explored in this study.The effect of leaching operation on the pore structure was investigated,and main factors affecting the seepage were analyzed.Results showed that the semiweathered layer presents a dense structure and a small number of unconnected pores.Pores of topsoil and weathered layers are mainly long and narrow column openings with some planar fractures.Even pore distribution and large size span were observed.Compared with the weathered layer,the topsoil layer demonstrates larger voids,smaller average pore volume and equivalent radius,and fewer coordination throats;however,the average equivalent radius of the throat in the topsoil layer is larger and largescale channels exist through ore body vertically.Hence,permeability of the topsoil layer is significantly higher than that of the weathered layer.Colloidal clay minerals migrate easily and the occurrence of silting in the small porosity blocks the throat and significantly decreases the permeability of the ore body in the leaching process.The equivalent radius of the throat is the key to the seepage.Reducing the migration of fine particles is an effective measure to protect the throat and shorten the leaching period. | Xianping Luo Yongbing Zhang Hepeng Zhou Kunzhong He Boyuan Zhang Dongming Zhang Weijing Xiao | 2022 | International Journal of Mining Science and Technology2022，32，2： | 5 |
| 5 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 6 | Inhibition characteristics of circulating current in parallel inverter driving system of mine hoist显示文摘 | Kuanfang He Yong Wang Dongming Xiao | 2016 | International Journal of Coal Science & Technology2016，3，1： | 3 |
| 7 | Periostin mediates epithelial-mesenchymal transition through the MAPK/ERK pathway in hepatoblastoma显示文摘Objective: The aim of the present study was to analyze the prognostic factors in patients with hepatoblastoma(HB) in our single center and to evaluate periostin(POSTN) expression in HB and its association with clinicopathological variables. In addition, the underlying mechanism of how POSTN promotes HB progression was discussed.Methods: POSTN expression was investigated in HB tumors by immunohistochemistry(IHC), immunofluorescence(IF) and Western blot(WB). The association among POSTN expression, clinicopathological features and overall survival(OS) was also evaluated. The migration and adhesion ability of HB cells were measured using chemotaxis and cell-matrix adhesion assays,respectively. Epithelial-mesenchymal transition(EMT)-associated markers and activation of the ERK pathway were detected by WB.Results: HB patients had poor prognosis which displayed lymph node metastasis, vascular invasion, POSTN and vimentin expression. POSTN expression was also associated with lymph node metastasis. Furthermore, overexpressed POSTN promoted migration and the adhesive ability of HB cells in vitro. In addition, we demonstrated that POSTN activated the MAPK/ERK pathway, upregulated the expression of Snail and decreased the expression of OVOL2. Finally, POSTN promoted the expression of EMT-associated markers.Conclusions: POSTN might modulate EMT via the ERK signaling pathway, thereby promoting cellular migration and invasion.Our study also suggests that POSTN may serve as a therapeutic biomarker in HB patients. | Lu Chen Xiangdong Tian Wenchen Gong Bo Sun Guangtao Li Dongming Liu Piao Guo Yuchao He Ziye Chen Yuren Xia Tianqiang Song Hua Guo | 2019 | Cancer Biology & Medicine2019，16，1： | 3 |
| 8 | Cytosolic phospholipase A2α modulates cell-matrix adhesion via the FAK/paxillin pathway in hepatocellular carcinoma显示文摘Objective: To explore the effect of cytosolic phospholipase A2α(cPLA2α) on hepatocellular carcinoma(HCC) cell adhesion and the underlying mechanisms.Methods: Cell adhesion, detachment, and hanging-drop assays were utilized to examine the effect of cPLA2α on the cell-matrix and cell-cell adhesion. Downstream substrates and effectors of cPLA2α were screened via a phospho-antibody microarray.Associated signaling pathways were identified by the functional annotation tool DAVID. Candidate proteins were verified using Western blot and colocalization was investigated via immunofluorescence. Western blot and immunohistochemistry were used to detect protein expression in HCC tissues. Prognosis evaluation was conducted using Kaplan-Meier and Cox-proportional hazards regression analyses.Results: Our findings showed that cPLA2α knockdown decreases cell-matrix adhesion but increases cell-cell adhesion in HepG2 cells. Microarray analysis revealed that phosphorylation of multiple proteins at specific sites were regulated by cPLA2α. These phosphorylated proteins were involved in various biological processes. In addition, our results indicated that the focal adhesion pathway was highly enriched in the cPLA2α-relevant signaling pathway. Furthermore, cPLA2α was found to elevate phosphorylation levels of FAK and paxillin, two crucial components of focal adhesion. Moreover, localization of p-FAK to focal adhesions in the plasma membrane was significantly reduced with the downregulation of cPLA2α. Clinically, cPLA2α expression was positively correlated with p-FAK levels. Additionally, high expression of both cPLA2α and p-FAK predicted the worst prognoses for HCC patients.Conclusions: Our study indicated that cPLA2α may promote cell-matrix adhesion via the FAK/paxillin pathway, which partly explains the malignant cPLA2α phenotype seen in HCC. | Piao Guo Yuchao He Lu Chen Lisha Qi Dongming Liu Ziye Chen Manyu Xiao Liwei Chen Yi Luo Ning Zhang Hua Guo | 2019 | Cancer Biology & Medicine2019，16，2： | 2 |
| 9 | Orderly coexistence and accumulation models of conventional and unconventional hydrocarbons in Lower Permian Fengcheng Formation,Mahu sag,Junggar Basin显示文摘By using the latest geological,seismic,drilling and logging data,this article studies the basic conditions for the formation of the total petroleum system and the orderly coexisting characteristics and accumulation models of conventional&unconventional reservoirs in the Lower Permian Fengcheng Formation in the Junggar Basin.Controlled by thermal evolution,hydrocarbon generation and expulsion process of the high-quality source rocks in alkaline lake as well as the characteristics of multi-type reservoirs(conglomerate,sandstone,dolomite and shale),conventional structure-lithologic reservoirs and tight oil and shale oil reservoirs controlled by source-reservoir structure have been formed.On the plane,mature conventional reservoirs,medium-high mature tight oil,and medium-high mature shale oil reservoirs coexist orderly from the slope area around Mahu sag to the sag.Based on the orderly coexisting characteristics of conventional and unconventional reservoirs in the Fengcheng Formation,it is clear that oil and gas in the Fengcheng Formation accumulate continuously over a large area in three accumulation models:integrated source-reservoir,source-reservoir in close contact,and separated source-reservoir model.The three accumulation models differ in relationship between source-reservoir structure,reservoir lithology and spatial distribution,hydrocarbon migration,oil and gas type.It is pointed out that the conventional&unconventional oil and gas should be explored and developed as a whole to achieve an overall breakthrough of the total petroleum system.This study is expected to enrich the geological theory of oil and gas enrichment in continental basins and to provide an analogy for exploration and research in other hydrocarbon-rich sags. | ZHI Dongming TANG Yong HE Wenjun GUO Xuguang ZHENG Menglin HUANG Liliang | 2021 | Petroleum Exploration and Development2021，48，1： | 2 |
| 10 | Novel P22-monoclonal antibody based blocking ELISA for the detection of African swine fever virus antibodies in serum显示文摘African swine fever(ASF)is a highly infectious,transboundary viral disease of domestic and wild pigs,and is currently the most serious threat to world swine production,resulting in significant economic loss.In the absence of vaccines and treatments,the control of the disease entirely depends on accurate and early diagnosis accompanied by the culling of infected pigs.Thus,a highly specific and sensitive diagnostic assay is required during an outbreak and surveillance of the disease.In this study,a highly sensitive,specific,rapid and repeatable P22-monoclonal antibody-based blocking enzyme-linked immunosorbent assay(bELISA)assay was developed for the detection of antibodies against genotype I and II African swine fever viruses(ASFVs).A total of 806 pig serum samples were tested to evaluate the performance of the diagnostic assay.To determine the PI(percent Inhibition)cut-off value,receiver-operating characteristic(ROC)analysis was applied.According to the ROC analysis of the data,98.10%specificity and 100%sensitivity were recorded when the threshold cut-off value of PI was established at 47%.In addition,the assay was able to detect ASFV antibodies as early as 9 days post-infection when serum samples from experimentally infected pigs were used.Taking all together,the results of the present study indicated that the P22-mAb based bELISA assay can be used for rapid and accurate detection of antibodies against ASFV,which could play a valuable role in the containment and prevention of ASFV as an alternative to other serological diagnostic methods.Also,this study will assist researchers to further investigate the immunogenic importance of P22 protein in ASFV infection. | Ghebremedhin Tsegay Weldu Tesfagaber Yuanmao Zhu Xijun He Wan Wang Zhenjiang Zhang Encheng Sun Jinya Zhang Yuntao Guan Fang Li Renqiang Liu Zhigao Bu Dongming Zhao | 2022 | Biosafety and Health2022，4，4： | 2 |
| 11 | Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda显示文摘The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in China.Because of its rapid spread and great risks in China,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management strategies.Here,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and China.Strain identification of 163 individuals collected from America,Africa and China showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the Chinese and African populations.Moreover,population geno-mics analysis showed that populations from Africa and China have close relationship with significantly genetic differentiation from American populations.Taken toge-ther,FAWs invaded into China were most likely origi-nated from Africa.Comparative genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to FAW.Treatments of Chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the pesticides.These findings will be useful in developing effective strategies for manage-ment of FAW in China and other invaded areas. | Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu Yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan Jiang Yongwei Zhang Hanhong Xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang | 2022 | Protein & Cell2022，13，7： | 2 |
| 12 | Characterization of deep ground geothermal field in Jiahe Coal Mine显示文摘Research into the characteristics of geothermal fields is important for the control of heat damage in mines. Based on measured geothermal data of boreholes from 200 m to 1200 m in a Jiahe Coal Mine, we demonstrate non-linear but increasing relations of both geo-temperatures and geothermal gradients with increases depth. Numerically, we fitted the relationship between geo-temperatures and depth, a first-order exponential decay curve, formulated as: T(h) = 4.975 + 23.08 exp(h/1736.1). | Zhang Yi Guo Dongming He Manchao Jiang Yaodong Yang Ching | 2011 | Mining Science and Technology2011，21，3： | 2 |
| 13 | Tumor microenvironment responsive drug delivery systems显示文摘Conventional tumor-targeted drug delivery systems(DDSs)face challenges,such as unsatisfied systemic circulation,low targeting efficiency,poor tumoral penetration,and uncontrolled drug release.Recently,tumor cellular molecules-triggered DDSs have aroused great interests in addressing such dilemmas.With the introduction of several additional functionalities,the properties of these smart DDSs including size,surface charge and ligand exposure can response to different tumor microenvironments for a more efficient tumor targeting,and eventually achieve desired drug release for an optimized therapeutic efficiency.This review highlights the recent research progresses on smart tumor environment responsive drug delivery systems for targeted drug delivery.Dynamic targeting strategies and functional moieties sensitive to a variety of tumor cellular stimuli,including pH,glutathione,adenosine-triphosphate,reactive oxygen species,enzyme and inflammatory factors are summarized.Special emphasis of this review is placed on their responsive mechanisms,drug loading models,drawbacks and merits.Several typical multi-stimuli responsive DDSs are listed.And the main challenges and potential future development are discussed. | Qunye He Jun Chen Jianhua Yan Shundong Cai Hongjie Xiong Yanfei Liu Dongming Peng Miao Mo Zhenbao Liu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 2 |
| 14 | Study of LED array fill light based on parallel particle swarm optimization in greenhouse planting显示文摘Agricultural productivity is crucial to the economy.The output and quality of crops have a direct impact on people’s daily lives and a country’s food and clothing.Therefore,harvesting high-quality crops efficiently and maximizing yield per unit area are the most important goals pursued by farmers.As an important parameter of plant growth,light intensity is one of the important factors that affects plant growth and development,morphological establishment and accumulation of functional chemical substances.When light intensity cannot meet the plant’s needs,the optimal light intensity or uneven light distribution will have a greater impact on plant growth and development.This paper aims to address the optimal plant light intensity problem.The paper presents an expert system technology database storing the empirical value of real-time light intensity values and compares it with a parallel particle swarm optimization algorithm to identify the optimal locations where LED lights need to be turned on and where drive circuit lit LED arrays need to be situated,to identify the number of LED fill lights and to solve light intensity optimization problems. | Feifei He Lihua Zeng Dongming Li Zhenhui Ren | 2019 | Information Processing in Agriculture2019，6，1： | 1 |
| 15 | Effects of cerebral ischemia-reperfusion on pharmacokinetic fate of paeoniflorin after intravenous administration of Paeoniae Radix extract in rats显示文摘 | He Xihui Xin g Dongming Ding Yi | 2004 | J Ethnopharm2004，94，23： | 1 |
| 16 | Photo-irradiation for preparation, modification and stimulation of polymeric membranes显示文摘 | He Dongming Susanto H Ulbricht M | 2009 | Progress in Polymer Science2009，34，1： | 1 |
| 17 | Raman and UV-visible absorption spectra of ionpaired aggregates of copper porphyrings 显示文摘 | Chen Dongming Zhang Yinghui He Tianjing | 2002 | Spectrochimica Acta Part A2002，58，： | 1 |
| 18 | Sisal pretreatment of thermoplastic starch composites mechanical properties and degradation properties显示文摘 | He Hezhi Wang Kexiang Yao Dongming | 2013 | The influence of plastic science and technology2013，9，2： | 1 |
| 19 | Effects of cerebral ischemia-reperfusion on pharmacokinetic fate of paeoniflorin after intravenous administration of Paeoniae Radix extract in rats显示文摘 | He Xihui Xing Dongming Ding Yi | 2004 | J Ethnopharmacol2004，94，： | 1 |
| 20 | Raman and UV-visible absorption spectra of ion-paired aggregates of copper porphyrins显示文摘 | CHEN Dongming ZHANG Yinghui HE Tianjing | 2002 | Spectrochimica Acta Part A2002，58，： | 1 |
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