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| 1 | Comparison of alternative extraction methods for secretome profiling in human hepatocellular carcinoma cells显示文摘Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media, which are rich in salts and other compounds that interfere with most proteomics techniques, presents a problem for secretome studies. Ultrafiltration, precipitation, and dialysis are three major extraction methods that can be used to overcome this problem. The present study for the first time, compared the merits and shortcomings of these three methods, without bias. Centrifugal ultrafiltration provided the best extraction efficiency, and precipitation provided the highest number of identifiable proteins. The three methods yielded closely related, but different, information on the secretome; thus, they should be considered complementary or, at least, supplementary methods. Three hundred and sixty unique proteins were identified, including 211 potential secreted proteins. Compared with previous studies, this study also identified 42 new secreted proteins. The present study not only offers a reference for the selection of secretome extraction methods, but also expands the secretome database for the investigation of hepatocellular carcinoma. | CAO Jing SHEN ChengPin ZHANG Jun YAO Jun SHEN HuaLi LIU YinKun LU HaoJie YANG PengYuan | 2011 | Science China(Life Sciences)2011，54，1： | 8 |
| 2 | Protein expression and fucosylated glycans of the serum haptoglobin-β subunit in hepatitis B virus-based liver diseases显示文摘Glycosylation,调整 glycoproteins 的配置和功能,是最重要的 translational 以后修正。学习是在 glycan 和浆液 haptoglobin- 子单元(Hp-) 的蛋白质部分观察微分模式的这的目的与肝炎 B 从病人净化了病毒(HBV ) 感染,肝肝硬化(LC ) ,或 hepatocellular 癌(HCC ) 。2-D 胶化电气泳动和多路的 proteomics 染色技术被采用调查 Hp- glycan 水平是否与蛋白质水平成正比。Multi-lectin 污点,高效的液体层析(HPLC ) ,和西方的污点分析被执行识别 Hp- 份量上的 glycoform。我们的实验证明到 Hp- 的 glycosylated 形式的全部的浆液 Hp- 的比率与不同的肝疾病在病人之中变化了。全部的 Hp- 蛋白质表示水平比 LC 在 HCC 是高得多的,当 fucosylated Hp- 的一个增长比例也在 HBV 和健康控制与那相比在 LC 和 HCC 病人被观察时。微分 fucosylation 被 HPLC 和反人的 SialylLewis X 抗体进一步作为路易斯十世结构识别。在结论, Hp- glycan 和全部的蛋白质表示的异常引申可以是为早 hepatocarcinogenesis 的有希望的 biomarker。 | Hong Shu Shu Zhang Xiaonan Kang Shan Li Xue Qin Chun Sun Haojie Lu Yinkun Liu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，7： | 6 |
| 3 | Serum paraoxonase 1 heteroplasmon, a fucosylated, and sialylated glycoprotein in distinguishing early hepatocellular carcinoma from liver cirrhosis patients显示文摘浆液 glycoproteins 的异常 glycan 结构在 hepatocellular 癌(HCC ) 的不同阶段创造唯一的模式,它为 HCC 的早诊断提供潜在的 glycan biomarkers。在这研究,双人脚踏车 lectin 亲密关系层析使用 aleuria aurantia lectin ( AAL )和小麦细菌凝集素( WGA )被处理从 27 肝肝硬化( LC )和 27 个早 HCC 病人净化 fucosylated 和 sialylated 浆液 glycoproteins ,在哪个 122 glycoproteins 被液体层析双人脚踏车团 spectrometry ( LC-MSMS )最后外面屏蔽。在 LC-MSMS 识别的 122 蛋白质之中,他们中的 8 个仅仅在 HCC 浆液被识别, 6 仅仅在 LC 浆液存在。浆液 paraoxonase (PON1 ) 1 是从 47 个单个病人的 immunoprecipitated 并且在 LC 比那些在 HCC 浆液由 lectins,出现提高的 fucosylation 和 sialylation 弄污浆液。在为 AAL 反应的 PON1 的 ROC 曲线(AUROC ) 下面的区域在把早 HCC 区分开来与 LC 与 71.4% 的敏感和 94.7% 的特性是 0.892。同样, WGA 反应的 PON1 与 95.2% 的敏感和 78.9% 的特性有 0.902 的 AUROC。数据显示浆液 PON1 的 glycan 差别可能为把早 HCC 与 LC 病人区分开来提供同样潜在的 glycan biomarkers。 | Chun Sun Pei Chen Qiaopei Chen Lu Sun Xiaonan Kang Xue Qin Yinkun Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，9： | 6 |
| 4 | Quantitative analysis of site-specific N-glycans on sera haptoglobin βchain in liver diseases显示文摘在有临床的申请的潜力的 glycoproteins 的 N-glycans 的地点特定的描述是重要的。在我们的以前的报告,链被发现在肝疾病不同的 sera haptoglobin (Hp ) 的全面 N-glycans。Hp 链包含 N-glycosylation 的四个潜在的地点。在这研究,我们以一种地点特定的方式在 Hp 链上调查了 N-glycans 的潜在的变化。在象有肝炎 B 病毒( HBV )的病人一样的健康个人的 Sera Hp 链,肝肝硬化( LC )和 hepatocellular 癌( HCC )被净化,消化并且使遭到了到液体 chromatography-electrospray 电离高度精力碰撞分离团 spectrometry ,它允许鉴定和 glycopeptide 的结构决心,象在每 glycopeptide 上在场的 glycans 的相对 quantification 一样。量的结果表明 NLFLN207HSEN211ATAK 和 fucosylated 的 sialylation 根本组织在 HBV 病人和健康个人与那些相比在 LC 和 HCC 病人显著地增加的 glycopeptides。在各种各样的肝疾病的 Hp 链的一套不同 N-glycan 模式被决定了。因此, sialylated 和 Hp 链力量的 fucosylated glycoforms 与早 hepatocarcinogenesis 有关并且可能也作为为 LC 和 HCC 病人的新奇微分标记有用。 | Shu Zhang Kai Jiang Chun Sun Haojie Lu Yinkun Liu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 3 |
| 5 | The Significance of MMP-9 Over MMP-2 in HCC Invasiveness and Recurrence of Hepatocellular Carcinoma After Curative Resection显示文摘 | Rongxin Chen Jiefeng Cui Changde Xu Tongchun Xue Kun Guo Dongmei Gao Yinkun Liu Shenglong Ye Zhenggang Ren | 2012 | Annals of Surgical Oncology2012，，3： | 2 |
| 6 | Prevalence and risk factors of hypertension based on repeated measurements in Chinese children and adolescents显示文摘 | Linghui Meng Yajun Liang Junting Liu Yuehua Hu Yinkun Yan Jie Mi | 2013 | Blood Pressure2013，，1： | 1 |
| 7 | Active canopy sensor-based precision N management strategy for rice显示文摘 | Yinkun Yao Yuxin Miao Shanyu Huang Lei Gao Xiaobo Ma Guangming Zhao Rongfeng Jiang Xinping Chen Fusuo Zhang Kang Yu Martin Gnyp Georg Bareth Cheng Liu Liqin Zhao Wen Yang Huamin Zhu | 2012 | Agronomy for Sustainable Development2012，，4： | 1 |
| 8 | Differentialpro-teomic analysis ofhuman hepatocellular carcinoma celllinemetastasis-associ-ated proteins显示文摘 | Cui Jiefeng Liu Yinkun Pan Boshen | 2002 | J Cancer Res Clin Oncol2002，130，10： | 1 |
| 9 | Tumor-derived secretory clusterin induces epithelial–mesenchymal transition and facilitates hepatocellular carcinoma metastasis显示文摘 | Cun Wang Kai Jiang Xiaonan Kang Dongmei Gao Chun Sun Yan Li Lu Sun Shu Zhang Xiaohui Liu Weizhong Wu Pengyuan Yang Kun Guo Yinkun Liu | 2012 | International Journal of Biochemistry and Cell Biology2012，，12： | 1 |
| 10 | Mutation and overexpression of the β-catenin gene may play an important role in primary hepatocellular carcinoma among Chinese people显示文摘 | Jian Cui Xinda Zhou Yinkun Liu Zhaoyou Tang | 2001 | Journal of Cancer Research and Clinical Oncology2001，，9： | 1 |
| 11 | Prediction of chronic hepatitis B, liver cirrhosis and hepatocellular carcinoma by SELDI-based serum decision tree classification显示文摘 | Jiefeng Cui Xiaonan Kang Zhi Dai Cheng Huang Haijun Zhou Kun Guo Yan Li Yu Zhang Ruixia Sun Jie Chen Yang Li Zhaoyou Tang Toshimasa Uemura Yinkun Liu | 2007 | Journal of Cancer Research and Clinical Oncology2007，，11： | 1 |
| 12 | The impact of body weight trajectory from childhood on chronic inflammation in adulthood: The Bogalusa Heart Study显示文摘Importance:The impact of long-term burden of excessive body weight,beginning in childhood,on inflammatory status in adulthood has been poorly described.Objective:To characterize the longitudinal body mass index(BMI)trajectory from childhood and examine its relationship with inflammatory status in adulthood.Methods:We included 1285 adults who had 4-15 repeat measurements of BMI from childhood to adulthood.The area under the curve(AUC)of growth curves was calculated to characterize long-term burden(total AUC)and trends(incremental AUC)of BMI.Results:After adjusting for covariates,higher values of BMI in terms of childhood and adulthood,as well as total and incremental AUC,were strongly associated with elevated levels of adult C-reactive protein(CRP)in the four race-sex groups.There were significant differences in linear and nonlinear curve parameters between the normal and high CRP groups for all race-sex groups(P<0.01).Compared with participants who had consistently low BMI in both childhood and adulthood,participants with high BMI in adulthood had higher CRP levels(P<0.001),irrespective of their childhood BMI status;participants with high BMI in childhood but low BMI in adulthood had similar adult CRP levels.Interpretation:The impact of excessive body weight on inflammation is cumulative and exacerbated over time.The influence of childhood overweight/obesity on inflammatory status in adulthood can be alleviated by reducing adiposity in adulthood. | Yinkun Yan Shengxu Li Yang Liu Lydia Bazzano Jiang He Jie Mi Wei Chen | 2021 | Pediatric Investigation2021，5，1： | 0 |
| 13 | Characteristics of pediatric inpatients with primary and secondary hypertension显示文摘Importance:Extensive population-based studies have explored the prevalence of primary hypertension(HTN)in children and adolescents.However,there is little published data on the characteristics of different types of pediatric HTN and the causes of secondary HTN.Objective:To investigate the characteristics of different types of pediatric HTN and the causes of secondary HTN in a hospital setting.Methods:The study cohort comprised pediatric inpatients(<18 years of age)discharged with a diagnosis of HTN from Beijing Children's Hospital during 2015-2020.Pediatric patients with HTN were allocated to secondary and primary HTN groups on the basis of comprehensive analyses of their diagnoses,family history of HTN,and findings on physical examination,as documented in their medical records.The Mann-Whitney U test,χ^(2) and Fisher's exact test were used to assess differences in characteristics of patients with different HTN types and causes of secondary HTN.Results:Data of 1470 inpatients with HTN from 18 clinical departments were included in the analysis.Among them,458(31.2%)had primary HTN,and 1012(68.8%)had secondary HTN.Compared with patients had primary HTN,children with secondary HTN were younger and had lower body mass indexes and longer lengths of stay.Moreover,children with primary HTN had mostly been managed by the Endocrinology and Cardiology Departments,75.8%of them having obesity-related comorbidities.In contrast,most patients with secondary HTN had been managed by the Nephrology Department,renal diseases being the leading cause of their HTN(46.3%).Interpretation:Secondary HTN is more common than primary HTN in pediatric clinical settings,renal diseases being the leading cause of secondary HTN. | Yijun Chen Peiyu Ye Xiaorong Liu Chunxiu Gong Caifeng Li Yue Yuan Huyong Zheng Xin Xu Hongbo Dong Qin Kong Yinkun Yan Jie Mi | 2021 | Pediatric Investigation2021，5，1： | 0 |
| 14 | Reliability and consistency assessment of land cover products atmacro and local scales in typical cities显示文摘Urban areas have higher heterogeneity compared to natural areas,it is crucial to assessfine-resolution land cover products and discover how they differ in urban areas so that they can be efficiently used for various application scenarios.In this study,five typical cities in China were chosen as study areas to evaluate four commonly used 30 m land cover products:GLC_FCS30-2020,FROM-GLC30-2017,Globeland30-2020,and CLCD-2019.We analyzed the reliability of these four products using validation samples as well as by examining their area and spatial pattern consistency.Given the limitations of traditional accuracy assessments at the macro level,we added a local area evaluation to further examine the classification details in these products.The macro results indicated that four land cover products within urban areas have a similar overall accuracy,surpassing 76%,but there was a low consistency among them,ranging from 42.21%to 61.13%.The local accuracy assessment illustrated that GLC_FCS30-2020 and FROM-GLC30-2017 performed well in reflecting the intricate details of the city,however,the four products exhibited varying degrees of misclassifications and omissions.These phenomena suggest that more sophisticated algorithms are needed to consider urban particularities sincefine-resolution land cover products may fail to capture complex urban details. | Wenxi Shi Xiang Zhao Jiacheng Zhao Siqing Zhao Yinkun Guo Naijing Liu Na Sun Xiaozheng Du Mengmeng Sun | 2023 | International Journal of Digital Earth2023，16，1： | 0 |
| 15 | Effects of galectin-3 inhibition on endometrial cell cycle and adhesion显示文摘Galectin-3(gal-3)and its ligands have been implicated in cell transformation and cancer metastasis.Gal-3 protein has been found in uterine epithelial cells adjacent to implanting blastocysts in different cell types.In order to investigate the role of gal-3 in the establishment of human endometrial receptivity,the expression of gal-3 in human endometrial cell line RL95-2 was silenced by RNA inter-ference technology using gal-3 specific small RNA.The expression of gal-3 was detected by the reverse transcriptase-polymerase chain reaction and Western blot analysis.After the suppression of gal-3,cell cycle changes and the expres-sion of integrin b1 were detected by flow cytometry.The adhesive ability of RL95-2 cells was analyzed by the adhesion test.Gal-3 siRNA transfection efficiency reached 70%–90%.The expression of gal-3 mRNA and protein in RL95-2 cells was strongly inhibited by 70%–90%after RNA interference.Inhibition of gal-3 expression decreased S-phase but increased G1 phase cells.Integrin b1 expression was down-regulated,and the adhesive ability of RL95-2 cells to fibronectin(FN)was significantly reduced.Gal-3 may be involved in the establishment of endometrial receptivity by regulating the proliferation and adhesion of endometrial cells.The influence on adhesion may be related with the integrin modulation. | LEI Caixia ZHANG Wei SUN Xiaowei DU Guoping WANG Li LIU Yinkun | 2007 | Frontiers of Medicine2007，1，4： | 0 |
| 16 | A four-channel time-interleaved 30-GS/s 6-bit ADC in 0.18 μm SiGe BiCMOS technology显示文摘Dear editor,Analog-to-digital converters(ADCs)with GHz bandwidth have widely been used in wideband communication systems,data acquisition systems and test and measurement instruments.As key elements of these systems,wideband high speed ADCs in multi-GHz range are in increasing demands.Folding and interpolating ADCs are perfectly suitable to these performance because | Xiaoge ZHU Danyu WU Lei ZHOU Yinkun HUANG Jin WU Xinyu LIU | 2017 | Science China(Information Sciences)2017，60，12： | 0 |
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