|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | The expression and binding properties of the rice WRKY68 protein in the Xa21-mediated resistance response to Xanthomonas oryzae pv. Oryzae显示文摘Plant WRKY transcription factors are involved in various physiological processes, including biotic and abiotic stress responses, as well as developmental processes. In this study, the expression patterns of the WRKY68 protein during interactions between rice 4021 containing the bacterial blight resistance gene Xa21 and Xanthomonas oryzae pv. oryzae(Xoo) were investigated. A possible modified form of the WRKY68 protein appeared in the Xa21-mediated disease resistance response, and its expression levels were similar in compatible and incompatible responses, but differed significantly from that of the mock control treatment, suggesting that WRKY68 may be involved in the bacterial blight response in rice. To further understand WRKY68's roles in the resistance signaling pathway, WRKY68 recombinant protein was expressed in Escherichia coli and a microscale thermophoresis analysis was performed to investigate the interactions between WRKY68 and cis-elements in crucial pathogenesis-related(PR) genes. The results showed that the WRKY68 protein binds to W-boxes in the PR1 b promoter region, with an apparent dissociation constant of 25 nmol L–1, while the binding between WRKY68 and PR10 a was W-box independent. The results suggested that a possible modified form of the WRKY68 protein was induced during the interaction between rice and Xoo, which then regulated the activity of the downstream PR genes by binding with the W-boxes in the PR1 b gene's promoter region. Moreover, the constitutive transcription of the WRKY68 gene in dozens of rice tissues and the expression of the WRKY68 protein in leaves during all growth stages suggests that WRKY68 plays important roles in rice during normal growth processes. | YANG Shuo ZHOU Liang MIAO Liu-yang SHI Jia-nan SUN Cai-qiang FAN Wei LAN Jin-ping CHEN Hao LIU Li-juan DOU Shi-juan LIU Guo-zhen LI Li-yun | 2016 | Journal of Integrative Agriculture2016，15，11： | 6 |
| 3 | Optimizing interfacial electronic coupling with metal oxide to activate inert polyaniline for superior electrocatalytic hydrogen generation显示文摘Tuning and optimization of electronic structures and related reaction energetics are critical toward the rational design of efficient electrocatalysts.Herein,experimental and theoretical calculation demonstrate the originally inert N site within polyaniline(PANI)can be activated for hydrogen evolution by proper d-πinterfacial electronic coupling with metal oxide.As a result,the assynthesized WO3 assemblies@PANI via a facile redox-induced assembly and in situ polymerization,exhibits the electrocatalytic production of hydrogen better than other control samples including W18O49@PANI and most of the reported nobel-metal-free electrocatalysts,with low overpotential of 74 mV at 10 mA·cm−2 and small Tafel slope of 46 mV·dec−1 in 0.5M H2SO4(comparable to commercial Pt/C).The general efficacy of this methodology is also validated by extension to other metal oxides such as MoO3 with similar improvements. | Zhen-Feng Huang Jiajia Song Yonghua Du Shuo Dou Libo Sun Wei Chen Kaidi Yuan Zhengfei Dai Xin Wang | 2019 | Carbon Energy2019，1，1： | 5 |
| 4 | Effect of Rotational Speed on the Stability of Two Rotating Side-by-side Circular Cylinders at Low Reynolds Number显示文摘Flow around two rotating side-by-side circular cylinders of equal diameter D is numerically studied at the Reynolds number 40≤ Re ≤200 and various rotation rate θ_i. The incoming flow is assumed to be two-dimensional laminar flow. The governing equations are the incompressible Navier-Stokes equations and solved by the finite volume method(FVM). The ratio of the center-to-center spacing to the cylinder diameter is T/D=2. The objective of the present work is to investigate the effect of rotational speed and Reynolds number on the stability of the flow.The simulation results are compared with the experimental data and a good agreement is achieved. The stability of the flow is analyzed by using the energy gradient theory, which produces the energy gradient function K to identify the region where the flow is the most prone to be destabilized and the degree of the destabilization. Numerical results reveal that K is the most significant at the separated shear layers of the cylinder pair. With Re increases,the length of the wake is shorter and the vortex shedding generally exhibits a symmetrical distribution forθ_i<θ_(crit), It is also shown that the unsteady vortex shedding can be suppressed by rotating the cylinders in the counter-rotating mode. | DOU Huashu ZHANG Shuo YANG Hui SETOGUCHI Toshiaki KINOUE Yoichi | 2018 | Journal of Thermal Science2018，27，2： | 3 |
| 5 | Dynamic regulation of innate lymphoid cells in the mucosal immune system显示文摘The mucosal immune system is considered a local immune system,a term that implies regional restriction.Mucosal tissues are continually exposed to a wide range of antigens.The regulation of mucosal immune cells is tightly associated with the progression of mucosal diseases.Innate lymphoid cells(ILCs)are abundant in mucosal barriers and serve as first-line defenses against pathogens.The subtype changes and translocation of ILCs are accompanied by the pathologic processes of mucosal diseases.Here,we review the plasticity and circulation of ILCs in the mucosal immune system under physiological and pathological conditions.We also discuss the signaling pathways involved in dynamic ILC changes and the related targets in mucosal diseases. | Fei Shao Dou Yu Pengyan Xia Shuo Wang | 2021 | Cellular & Molecular Immunology2021，18，6： | 2 |
| 6 | Molecular diagnosis and treatment of meningiomas:an expert consensus(2022)显示文摘Meningiomas are the most common primary intracranial neoplasm with diverse pathological types and complicated clinical manifestations.The fifth edition of the WHO Classification of Tumors of the Central Nervous System(WHO CNS5),published in 2021,introduces major changes that advance the role of molecular diagnostics in meningiomas.To follow the revision of WHO CNS5,this expert consensus statement was formed jointly by the Group of Neuro-Oncology,Society of Neurosurgery,Chinese Medical Association together with neuropathologists and evidence-based experts.The consensus provides reference points to integrate key biomarkers into stratification and clinical decision making for meningioma patients. | Jiaojao Deng Lingyang Hua Liuguan Bian Hong Chen Ligang Chen Hongwei Cheng Changwu Dou Dangmurenjiapu Geng Tao Hong Hongming Ji Yugang Jiang Qing Lan Gang Li Zhixiong Liu Songtao Qi Yan Qu Songsheng Shi Xiaochuan Sun Hajun Wang Yongping You Hualin Yu Shuyuan Yue Jianming Zhang Xiaohua Zhang Shuo Wang Ying Mao Ping Zhong Ye Gong Group of Neuro-Oncology Society of Neurosurgery Chinese Medical Association | 2022 | Chinese Medical Journal2022，135，16： | 1 |
| 7 | Remediating fluoride from water using hydrous zirconium oxide显示文摘 | Xiaomin Dou Dinesh Mohan Charles U. Pittman Shuo Yang | 2012 | Chemical Engineering Journal2012，，： | 1 |
| 8 | The Zinc Finger Motif of Escherichia coli RecQ is Implicated in Both DNA Binding and Protein Folding 显示文摘 | LIU Jie lin RIGOLET P DOU Shuo xing | 2004 | J Biol Chem2004，279，42： | 1 |
| 9 | Planting uniformity performance of motor-driven maize precision seeding systems显示文摘Low accuracy planting uniformity affects yield.Seed meter type and forward speed typically interfere with the planting uniformity accuracy of motor-driven seeding systems.Two types of maize precision planters equipped with motor-driven planting systems are investigated in this study to ascertain the rule of planting uniformity in both simulated and field speeds.The simulated speed increases from 5 to 12 km/h at a 1 km/h interval in a laboratory environment.The test results show that the quality of feed index(QTFI)of the two planters decreased by 16.79%and 9.88%.This is primarily attributed to the increase in the miss index(MISS)by 11.62%and 9.70%,respectively.The field speed was set to four levels from 5 to 12 km/h in a field environment.The plant spacing scatter distribution results were analyzed,and the results of the two planters indicated that the average positive difference of the two planters linearly increased with the forward speed,and the negative difference of the two planters did not exhibit a linear correlation.The number of positive moving average points was 2.49 times greater than that of the negative moving average points of the finger pick-up maize precision planter,and 4.49 times in the air-suction maize precision planter.The results indicated that the increase of the positive difference of plant spacing is the major effect factor in the field planting uniformity of the two motor-driven maize precision planters.In addition,the plant spacing corresponded to the distribution frequency of the two planters in field was close to the target seed spacing of 25 cm with a max coefficient of variation(CV)of 21.55%and 20.66%,respectively,and those plant spacing values corresponded to max distribution frequency of the two planters at the four level field speeds were(24.69±0.63)cm and(25.63±0.32)cm,respectively.However,the multiples index(MUL)changed randomly affected by the increasing speed.The research results provide a direction for the optimization design of motor-driven maize precision planters. | Shuo Yang Changyuan Zhai Yuanyuan Gao Hanjie Dou Xueguan Zhao Yakai He Xiu Wang | 2022 | International Journal of Agricultural and Biological Engineering2022，15，5： | 0 |
| 10 | Space advanced technology demonstration satellite显示文摘The Space Advanced Technology demonstration satellite(SATech-01),a mission for low-cost space science and new technology experiments,organized by Chinese Academy of Sciences(CAS),was successfully launched into a Sun-synchronous orbit at an altitude of~500 km on July 27,2022,from the Jiuquan Satellite Launch Centre.Serving as an experimental platform for space science exploration and the demonstration of advanced common technologies in orbit,SATech-01 is equipped with 16 experimental payloads,including the solar upper transition region imager(SUTRI),the lobster eye imager for astronomy(LEIA),the high energy burst searcher(HEBS),and a High Precision Magnetic Field Measurement System based on a CPT Magnetometer(CPT).It also incorporates an imager with freeform optics,an integrated thermal imaging sensor,and a multi-functional integrated imager,etc.This paper provides an overview of SATech-01,including a technical description of the satellite and its scientific payloads,along with their on-orbit performance. | ZHANG XiaoFeng CHEN Wen ZHU XiaoCheng MENG Na HE JunWang BI XingZi ZHANG YongHe SHI Qi LI Fei LIU Rui FENG ZhengGong LIU Liu LI JinSong WU HaiChen XU DongXiao LI TaiJie HUANG JiangJiang LIU Shuo LI TianTong YU XianSheng GAO Yang ZHOU Heng BAN HanYu ZHANG YanLi ZHANG YueTing YANG YingQuan HE Tao DUAN XuLiang CHEN Xin WANG YaMin SUN AnTai ZHANG KuoXiang SUN Ying WANG YaoBin FAN ChengCheng XIONG ShaoLin LI XinQiao WEN XiangYang LING ZhiXing SUN XiaoJin ZHANG Chen BAI XianYong WANG ZhanShan DENG YuanYong TIAN Hui YANG JianFeng XUE HongBo SANG Peng LIU JinGuo ZHENG HuiLong ZHU Xiang HE JianWu LI Hui XU LuXiang XU ShuYan CHEN WenWu LIU ZhenDong WANG ZhaoLi MAO XiangLong GAO Rong LI ZongXuan DING GuoPeng WANG XinYu DOU RunJiang WENG LuBin LUO Hao WANG YaPing LIANG XianFeng FANG ZiRuo | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 11 | The neuropeptide CGRP enters the macrophage cytosol to suppress the NLRP3 inflammasome during pulmonary infection显示文摘The NLRP3 inflammasome plays an essential role in resistance to bacterial infection. The nervous system secretes multiple neuropeptides affecting the nervous system as well as immune cells. The precise impact of the neuropeptide CGRP on NLRP3 inflammasome activation is still unclear. Here, we show that CGRP negatively regulates the antibacterial process of host cells. CGRP prevents NLRP3 inflammasome activation and reduces mature IL-1β secretion. Following NLRP3 inflammasome stimulation that triggers endosome leakage, CGRP internalized to endosomal compartments is released into the cell cytosol. Cytosolic CGRP binds directly to NLRP3 and dismantles the NLRP3-NEK7 complex, which is crucial for NLRP3 inflammasome activation. CGRP administration exacerbates bacterial infection, while the treatment with a CGRP antagonist has the opposite effect. Our study uncovers a unique role of CGRP in inhibiting inflammasome activation during infections, which might shed new light on antibacterial therapies in the future. | Fangrui Zhu Dou Yu Xiwen Qin Yan Qian Juan Ma Weitao Li Qiannv Liu Chunlei Wang Yan Zhang Yi Li Dong Jiang Shuo Wang Pengyan Xia | 2023 | Cellular & Molecular Immunology2023，20，3： | 0 |
| 12 | Highly permeable reverse osmosis membranes incorporated with hydrophilic polymers of intrinsic microporosity via interfacial polymerization显示文摘Enhancing the water permeation while maintaining high salt rejection of existing reverse osmosis(RO)membranes remains a considerable challenge.Herein,we proposed to introduce polymer of intrinsic microporosity,PIM-1,into the selective layer of reverse osmosis membranes to break the trade-off effect between permeability and selectivity.A water-soluble a-LPIM-1 of low-molecular-weight and hydroxyl terminals was synthesized.These designed characteristics endowed it with high solubility and reactivity.Then it was mixed with m-phenylenediamine and together served as aqueous monomer to react with organic monomer of trimesoyl chloride via interfacial polymerization.The characterization results exhibited that more“nodule”rather than“leaf”structure formed on RO membrane surface,which indicated that the introduction of the high free-volume of a-LPIM-1 with three dimensional twisted and folded structure into the selective layer effectively caused the frustrated packing between polymer chains.In virtue of this effect,even with reduced surface roughness and unchanged layer thickness,the water permeability of prepared reverse osmosis membranes increased 2.1 times to 62.8 L·m^(-2)·h^(-1) with acceptable Na Cl rejection of 97.6%.This attempt developed a new strategy to break the trade-off effect faced by traditional polyamide reverse osmosis membranes. | Jing Dou Shuo Han Saisai Lin Zhikan Yao Lian Hou Lin Zhang | 2022 | Chinese Journal of Chemical Engineering2022，35，5： | 0 |
| 13 | Magnetic field induced synthesis of (Ni, Zn)Fe_(2)O_(4) spinel nanorod for enhanced alkaline hydrogen evolution reaction显示文摘Recently, the introduction of external fields (light, thermal, magnetism, etc.) during electrocatalysis reactions gradually becomes a new strategy to modulate the catalytic activities. In this work, an external magnetic field was innovatively employed for the synthesis progress of (Ni, Zn)Fe_(2)O_(4) spinel oxide (M-(Ni, Zn)Fe_(2)O_(4)). Results indicated the magnetic field (≤250 mT) would affect the morphology of catalyst due to the existing Fe ions, inducing the M-(Ni, Zn)Fe_(2)O_(4) nanosphere particles to be uniform and coral-like nanorods. In addition, the electronic structure of the catalyst was regulated by the valence state of Fe, changing the bonding of metal to oxygen atoms in different spinel sites. The results manifested that the M-(Ni, Zn)Fe_(2)O_(4) requires a lower overpotential of only 67 mV to deliver 10 mA cm−2 for hydrogen evolution reaction (HER) in alkaline electrolyte. Moreover, M-(Ni, Zn)Fe_(2)O_(4) respectively as the anode and cathode electrode for the overall water splitting, the catalysis system requires a cell voltage of only 1.76 V to gain a current density of 50 mA cm−2, combining with an excellent discharging stability after 10 h. This work provides a facile synthesis strategy toward the design of efficient non-noble metal catalysts for alkaline HER and overall water splitting. | Mingkun Xia Yan zhang Jiangyue Xiao Peng Zhao Zhongjun Hou Fanglin Du Dawei Chen Shuo Dou | 2023 | Progress in Natural Science:Materials International2023，33，2： | 0 |
| 14 | Deciphering the regulatory and catalytic mechanisms of an unusual SAM-dependent enzyme显示文摘S-adenosyl-1-methionine(SAM)-dependent enzymes regulate various disease-related behaviors in all organisms.Recently,the leporin biosynthesis enzyme LepI,a SAM-dependent enzyme,was reported to catalyze pericyclic reactions in leporin biosynthesis;however,the mechanisms underlying LepI activation and catalysis remain unclear.This study aimed to investigate the molecular mechanisms of LepI.Here,we reported crystal structures of LepI bound to SAM/5′-deoxy-5′-(methylthio)adenosine(MTA),S-adenosyl-homocysteine(SAH),and SAM/substrate states.Structural and biochemical analysis revealed that MTA or SAH inhibited the enzyme activities,whereas SAM activated the enzyme.The analysis of the substrate-bound structure of LepI demonstrated that this enzymatic retro-Claisen rearrangement was primarily driven by three critical polar residues His133,Arg197,Arg295 around the active site and assisted by SAM with unclear mechanism.The present studies indicate that the unique mechanisms underlying regulatory and catalysis of the unusual SAM-dependent enzyme LepI,not only strengthening current understanding of the fundamentally biochemical catalysis,but also providing novel insights into the design of SAM-dependent enzyme-specific small molecules. | Qiu Sun Yuehong Hu Yijun Gu Jiangkun Huang Jun He Lan Luo Yi Yang Shuo Yin Chao Dou Tianqi Wang Xianghui Fu Ling He Shiqian Qi Xiaofeng Zhu Shengyong Yang Xiawei Wei Wei Cheng | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 0 |
| 15 | Comparison of Obesity Prevalence among Middle and High School Graduates before and after the COVID-19 Lockdown显示文摘The world has been engulfed in a COVID-19 pandemic that has significantly affected the health and economics of the population.The Chinese authorities imposed lockdown measures to limit the spread of COVID-19 and stopped school programs for children and adolescents.Such measures have been associated with increased sedentary time and reduced physical activity[1-3].An online questionnaire study of youth in China compared activity patterns before and after the COVID-19 lockdown. | LU Yan Hui DOU Zi Yan ZHAO Kai Ping YAN Dong CHEN Shuo CHENG Jun Xia Glen M.BLAKE Karen HIND ZHANG Jing Bo CHENG Xiao Guang WU Jing | 2022 | Biomedical and Environmental Sciences2022，35，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员