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| 1 | Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97显示文摘ALM To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. METHODS Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97. andbiological characteristics of the target clones selected by in vivo screening were studied.``RESULTS Two clones with high MHCC97-H and IowMHCC9--L1 metastatic potential were isolated from theparent cell line. Compared with MHCC97-L. MHCC97-H hadsmaller cell size average cell diameter 43 um vs 50 μmand faster in vitro and in vivo growth rate tumor celldoubling time was 34.2 h vs 60.0 h. The main ranges ofchromosomes were 5.5 58 in MHCC97-H and 57 62 inMHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was 137.5 - 11 .0) cellsfield for MHC_C99--H vs 17.7 - 6.3) field for MHCC97-L.The proportions of cells in GO Gl phase. S phase, and G_ M phase for MHCC97-H MHCC97-L were 0.56 6.65.0.28 0.25 and 0.l6 0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5 wk after orthotopic implantation of tumor tissue were ( 24666 μg. L for MHCC97-H and (91- 66) μg' L 1 for MHCC97L. The pulmonary metastatic rate was 100% (10-10) vs40% 4- 10).``CONCLUSION Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis. | Yan Li Zhao-You Tang Sheng-Long Ye Yin-Kun Liu Jie Chen Qiong Xue Jun Chen Dong-Mei Gao Wei-Hua Bao Liver Cancer Institute and Zhongshan Hospital of Fudan University (Former Liver Cancer Institute of Shanghai Medical University),Shanghai 200032,China | 2001 | World Journal of Gastroenterology2001，7，5： | 111 |
| 2 | Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential显示文摘Metastatic human HCC model is needed for the studies on mechanism and intervention of metastatic recurrence. By using orthotopic implantation of histologically intact tissues of 30 surgical specimens, a patient like metastatic model of human HCC in nude mice (LCI-D20)and a Iow metastatic model of human HCC in nude mice LCI-D35 ) have been established. All mice with transplanted LCI-D20 tumors exhibited extremely high metastatic ability including spontaneous metastasis to liver, lungs, lymph nodes and peritoneal seeding.Remarkable difference was also found in expression of some of the invasiveness related genes and growth factors between the LCI-D20 and LCI-D35 tumors. PAI-Iincreased gradually following tumor progression in LCID20 model, and correlated with tumor size and AFP level,Phasic expression of tissue intercellular adhesion molecule-I in this model was also observed. Using corneal micropocket model, it was demonstrated that the vascular response induced by LCI-D20 tumor was stronger than that induced by LCI-D35 tumor. Similar report on metastatic human HCC model in nude mice and human HCC cell line with metastatic potential was rarely found in the literature. This LCI-D20 model has been widely used for the studies on intervention of metastasis, including antiangiogenesis, antisense approach, metalloproteinase inhibitor, differentiation inducer, etc. It is concluded that the establishment of metastatic human HCC model in nude mice and human HCC cell line with metastatic potential will provide important models for the in vivo and in vitro study of HCC invasiveness, angiogenesis as well as intervention of HCC recurrence. | Zhao-You Tang Fan-Xian Sun Jian Tian Sheng-Long Ye Yin-Kun Liu Kang-Da Liu Qiong Xue Jie Chen Jing-Lin Xia Lun-Xiu Qin Hui-Chuan Sun Lu Wang Jian Zhou Yan Li Zeng-Chen Ma Xin-Da Zhou Zhi-Quan Wu Zhi-Ying Lin Bing-Hui Yang Liver Cancer Institute of Fudan University and Zhongshan Hospital,Shanghai 200032,China | 2001 | World Journal of Gastroenterology2001，7，5： | 34 |
| 3 | Exotic origin of the Chinese continental shelf: new insights into the tectonic evolution of the western Pacific and eastern China since the Mesozoic显示文摘The effect of paleo-Pacific subduction on the geological evolution of the western Pacific and continental China is likely complex. Nevertheless, our analysis of the distribution of Mesozoic granitoids in the eastern continental China in space and time has led us to an interesting conclusion: The basement of the continental shelf beneath East and South China Seas may actually be of exotic origin geologically unrelated to the continental lithosphere of eastern China. By accepting the notion that the Jurassic–Cretaceous granitoids in the region are genetically associated with western Pacific subduction and the concept that subduction may cease to continue only if the trench is being jammed, then the termination of the granitoidmagmatism throughout the vast region at *88 ± 2 Ma manifests the likelihood of ‘‘sudden'', or shortly beforehand(*100 Ma), trench jam of the Mesozoic western Pacific subduction. Trench jam happens if the incoming‘‘plate'' or portion of the plate contains a sizeable mass that is too buoyant to subduct. The best candidate for such a buoyant and unsubductable mass is either an oceanic plateau or a micro-continent. We hypothesize that the basement of the Chinese continental shelf represents such an exotic, buoyant and unsubductable mass, rather than seaward extension of the continental lithosphere of eastern China. The locus of the jammed trench(i.e., the suture) is predictably located on the shelf in the vicinity of, and parallel to, the arc-curved coastal line of the southeast continental China. It is not straightforward to locate the locus in the northern section of the East China Sea shelf because of the more recent(\20 Ma) tectonic re-organization associated with the opening of the Sea of Japan. We predict that the trench jam at *100 Ma led to the re-orientation of the Pacific plate motion in the course of NNW direction as inferred from the age-progressive Emperor Seamount Chain of Hawaiian hotspot origin(its oldest unsubdued Meiji and Detroit seamounts are *82 Ma),making the boundary between the Pacific plate and the newly accreted plate of eastern Asia a transform fault at the location east of the continental shelf of exotic origin. This explains the apparent *40 Myr magmatic gap from *88to *50 Ma prior to present-day western Pacific subduction initiation. We propose that basement penetration drilling on well-chosen sites is needed to test the hypothesis in order to reveal the true nature of the Chinese continental shelf basement. This testing becomes critical and cannot longer be neglected in order to genuinely understand the tectonic evolution of the western Pacific and its effect on the geology of eastern China since the Mesozoic, includingthe cratonic lithosphere thinning, related magmatism/mineralization, and the mechanism of the subsequent South China Sea opening, while also offering novel perspectives on aspects of the plate tectonics theory. We also suggest the importance of future plate tectonic reconstruction of the western Pacific to consider the nature and histories of the Chinese continental shelf of exotic origin as well as the probable transform plate boundary from *100 to*50 Ma. Effort is needed to reveal the true nature and origin of the *88 ± 2 Ma granitic gneisses in Taiwan and the 110–88 Ma granitoids on the Hainan Island. | Yaoling Niu Yi Liu Qiqi Xue Fengli Shao Shuo Chen Meng Duan Pengyuan Guo Hongmei Gong Yan Hu Zhenxing Hu Juanjuan Kong Jiyong Li Jinju Liu Pu Sun Wenli Sun Lei Ye Yuanyuan Xiao Yu Zhang | 2015 | Science Bulletin2015，60，18： | 32 |
| 4 | Development of Oryza rufipogon and O. sativa Introgression Lines and Assessment for Yield-related Quantitative Trait Loci显示文摘基因渗入线人口有效地在印射量的特点 loci (QTL ) 被使用,识别有利基因,发现隐藏的遗传变异,评估处于多重条件的 QTL 的行动或相互作用并且提供为植物繁殖和基因研究的有利试验性的材料。在这研究,先进回交和连续 selfing 策略被用来开发基因渗入线(IL ) ,它源于 Oryza rufipogon Griff 的就职。从 Yuanjiang 县镇定,中国的云南省,著名计算机生产厂商他施主,和精英 indica 栽培变种 Teqing (O。sativa L.) ,接受者。从 O 的 Introgressionsegments。rufipogon 用 179 次多态的简单顺序重复(SSR ) 被屏蔽在每 IL 的染色体的标记。基因渗入线人口带的 Introgressed 片断包含了盖住整个 O 的 120 IL。rufipogon 染色体。同型结合的 O 的吝啬的数字。rufipogonsegments 每基因渗入线是大约 3.88。introgressed 片断的平均长度是近似的 25.5 厘米,并且有的大约 20.8% 这些片断缩放不到 10 厘米。eachIL 的染色体怀有 O 的 chromosomal 碎片。rufipogon 从 0.54% ~ 23.7%,与 5.79% 的全面一般水准。在每个地点, O 的替换的比率。rufipogon 等位基因有范围 of1.67-9.33,与 5.50 的一般水准。大量改变在词法并且 yield-relatedtraits 也在基因渗入线人口被发现。用单个点的分析,为产量和产量构成因素的 37 通常认为的 QTL 的一个总数与解释 phenotypic 的 7%-20% 在二个地点被检测。十九 QTL (51.4%) 在两个地点被检测,并且从在十五 loci (40.5%) 的 O.rufipogon 的等位基因在 Teging 背景改进了收益和产量构成因素。这些 O。rufipogon-O。sativa 基因渗入线将为从普通野米饭识别并且使用有利基因用作遗传物质…… | Lubin Tan | 2007 | Journal of Integrative Plant Biology2007，49，6： | 28 |
| 5 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 6 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 7 | Changes in distribution and microstructure of bauxite residue aggregates following amendments addition显示文摘Bauxite residue is a highly alkaline byproduct which is routinely discarded at residue disposal areas. Improving soil formation process to revegetate the special degraded lands is a promising strategy for sustainable management of the refining industry. A laboratory incubation experiment was used to evaluate the effects of gypsum and vermicompost on stable aggregate formation of bauxite residue. Aggregate size distribution was quantified by fractal theory, whilst residue microstructure was determined by scanning electron microscopy and synchrotron-based X-ray micro-computed tomography. Amendments addition increased the content of macro-aggregates(> 250 μm) and enhanced aggregate stability of bauxite residue. Following gypsum and vermicompost addition, fractal dimension decreased from 2.84 to 2.77, which indicated a more homogeneous distribution of aggregate particles. Images from scanning electron microscopy and three-dimensional microstructure demonstrated that amendments stimulate the formation of improved structure in residue aggregates. Pore parameters including porosity, pore throat surface area, path length, and path tortuosity increased under amendment additions. Changes in aggregate size distribution and microstructure of bauxite residue indicated that additions of gypsum and vermicompost were beneficial to physical condition of bauxite residue which may enhance the ease of vegetation. | Shengguo Xue Yuzhen Ye Feng Zhu Qiongli Wang Jun Jiang William Hartley | 2019 | Journal of Environmental Sciences2019，31，4： | 21 |
| 8 | Fecal microbiota transplantation improves metabolism and gut microbiome composition in db/db mice显示文摘Fecal microbiota transplantation(FMT)has become an effective strategy to treat metabolic diseases,including type 2 diabetes mellitus(T2DM).We previously reported that the intestinal microbiome had significant difference between individuals with normal glucose tolerance and T2DM in Chinese Kazak ethnic group.In this study,we investigated the effects of transplanted fecal bacteria from Kazaks with normal glucose tolerance(KNGT)in db/db mice.The mice were treated with 0.2 mL of fecal bacteria solution from KNGT daily for 10 weeks.We showed that the fecal bacteria from KNGT successfully colonized in the intestinal tract of db/db mice detected on day 14.In the FMT-treated db/db mice,the levels of fasting blood glucose,postprandial glucose,total cholesterol,triglyceride,and low-density lipoprotein–cholesterol were significantly downregulated,whereas high-density lipoprotein–cholesterol levels were upregulated.In the FMT-treated db/db mice,Desulfovibrio and Clostridium coccoides levels in gut were significantly decreased,but the fecal levels of Akkermansia muciniphila and colon histone deacetylase-3(HDAC3)protein expression were increased.At 8 weeks,both intestinal target bacteria and HDAC3 were correlated with glycolipid levels;Akkermansia muciniphila level was positively correlated with HDAC3 protein expression(r=+0.620,P=0.037).Our results suggest that fecal bacteria from KNGT could potentially be used to treat diabetic patients. | Pei-pei Zhang Lin-lin Li Xue Han Qin-wei Li Xu-hua Zhang Johnson J.Liu Ye Wang | 2020 | Acta Pharmacologica Sinica2020，41，5： | 19 |
| 9 | Zoned Zircon from Eclogite Lenses in Marbles from the Dabie-Sulu UHP Terrane,China: A Clear Record of Ultra-deep Subduction and Fast Exhumation显示文摘摘要:在从 Dabie-Sulu 的云石的 Eclogite 透镜超离频压力(UHP ) 岩层是深深地 subducted 元沉积的岩石。在这些岩石中的锆石被用来抑制年龄与其他天体作同方并且 UHP 变态在 subduction 期间,并且以后后退变态在发掘期间。继承(由岩屑形成) 并且变形锆石根据播送轻显微镜学,阴极射线发光(CL ) 成像,踪迹元素内容和矿物质包括被区分。矿物质包括的分发与变形锆石的 CL 成像结合了使联系锆石地区(领域) 到不同变形舞台可能。域 1 由组成圆,长方形和细长纤弱的核心与黑暗光想象,并且包含石英 eclogite 外形矿物质包括集合,显示的形成在下面高压(HP ) T = 的变形条件 | LIU Fulai A. GERDES P. T. ROBINSON XUE Huaimin YE Jianguo | 2007 | Acta Geologica Sinica(English Edition)2007，81，2： | 18 |
| 10 | Identification and differentiation of Panax ginseng,Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers显示文摘To differentiate traditional Chinese medicines(TCM) derived from congeneric species in TCM compound preparations is usually challenging. The roots of Panax ginseng(PG), Panax quinquefolium(PQ) and Panax notoginseng(PN) are used as popular TCM. They contain similar triterpenoid saponins(ginsenosides) as the major bioactive constituents. Thus far, only a few chemical markers have been discovered to differentiate these three species. Herein we present a multiple marker detection approach to effectively differentiate the three Panax species, and to identify them in compound preparations. Firstly, 85 batches of crude drug samples(including 32 PG, 30 PQ, and 23 PN) were analyzed by monitoring 40 major ginsenosides in the extracted ion chromatograms(EICs) using a validated LC–MS fingerprinting method. Secondly, the samples were clustered into different groups by pattern recognition chemometric approaches using PLS-DA and OPLS-DA models, and17 diagnostic chemical markers were discovered. Aside from the previously known Rf and p-F11, ginsenoside Rs1 could be a new marker to differentiate PG from PQ. Finally, the above multiple chemical markers were used to identify the Panax species in 60 batches of TCM compound preparations. | Wenzhi Yang Xue Qiao Kai Li Jingran Fan Tao Bo De-an Guo Min Ye | 2016 | Acta Pharmaceutica Sinica B2016，6，6： | 18 |
| 11 | Overexpression and Immunosuppressive Functions of Transforming Growth Factor 1,Vascular Endothelial Growth Factor and Interleukin-10 in Epithelial Ovarian Cancer显示文摘Objective: Transforming growth factor-1 (TGF-β1), vascular endothelial growth factor (VEGF), and interleukin-10 (IL-10) may be critical cytokines in the microenvironment of a tumor, playing roles in immune suppression. This study was conducted to elucidate the roles and immunosuppressive functions of these cytokines in epithelial ovarian cancer (EOC). Methods: The expression levels of TGF-β1, VEGF and IL-10 in malignant tissue were evaluated by immune-histochemistry and compared with corresponding borderline, benign, and tumor-free tissues. Moreover, relationships among the levels of these cytokines and correlations between expression and the prognosis of EOC were analyzed by Pearson rank correlations and multi-factor Logistic regression. The roles of TGF-β1, VEGF, and IL-10 in the immunosuppressive microenvironment of ovarian cancer were studied through dendritic cell (DC) maturation and CD4+CD25+FoxP3+Treg generation in vitro experiments. Results: TGF-β1, VEGF, and IL-10 were expressed in 100%, 74.69%, and 54.96% of EOC patients, respectively. TGF-β1 was an independent prognostic factor for EOC. IL-10 was significantly co-expressed with VEGF. In vitro, VEGF and TGF-β1 strongly interfered with DC maturation and consequently led to immature DCs, which secreted high levels of IL-10 that accumulated around the tumor site. TGF-β1 and IL-10 induced Treg generation without antigen presentation in DCs. Conclusions: TGF-β1, VEGF and IL-10 play important roles in EOC and can lead to frequent immune evasion events. | Chan-zhen Liu Li Zhang Xiao-hong Chang Ye-xia Cheng Hong-yan Cheng Xue Ye Tian-yun Fu Jun Chen Heng Cui | 2012 | Chinese Journal of Cancer Research2012，24，2： | 13 |
| 12 | Syntheses and Fungicidal Activities of Thiazole-5-carboxanilides Bearing Thioether Group显示文摘 | XUE Hansong LIU Aiping LIU Weidong LI Jianming REN Yeguo HUANG Lu HE Lian OU Xiaoming YE Jiao HUANG Mingzhi | 2016 | Chemical Research in Chinese Universities2016，32，5： | 13 |
| 13 | Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer显示文摘背景人的 epididymis 能分泌的蛋白质(HE4 ) 4 被证明了是为上皮的卵巢的癌的察觉的有希望的新奇 biomarker。与 CA125 相比, HE4 试金表明了一个改进能力与恶意、良性的疾病在骨盆的质量之间区别。尽管然而, HE4 是在卵巢的癌症的 overexpressed,是众所周知的,在卵巢的癌症的 carcinogenesis 和前进的 HE4 的角色在这研究仍然是 unkown.Methods,我们在卵巢的癌症的 carcinogenesis 和前进探索了 HE4 的角色。我们为 HE4 表示,和使用的 RNA 干扰(RNAi ) 屏蔽了九根卵巢的癌症房间线,我们在 CaoV3 和 SKOV3.ip1 的 silenced HE4 基因表示卵巢的癌症房间线。我们由在 vitro.Results HE4 基因 silencing 检验房间周期, apoptosis,增长和 transwell 迁居 / 侵略在转变显型上估计了 HE4 基因 silencing 的效果导致 G0/G1 拘捕并且在 CaoV3 和 SKOV3.ip1 房间阻止 G1 接近前进到 S 阶段。HE4 在在 vitro.Conclusion HE4 的 SKOV3.ip1 房间的击倒的也禁止的房间增长,移植和侵略可以涉及房间周期的规定并且支持卵巢的癌症移植和侵略。 | ZOU Shu-li CHANG Xiao-hong YE Xue CHENG Hong-yan CHENG Ye-xia TANG Zhi-jian ZHANG Zu-juan GAO Li CHEN Xin-hua CUI Heng | 2011 | Chinese Medical Journal2011，，19： | 12 |
| 14 | Advances in China's Organ Transplantation Achieved with the Guidance of Law显示文摘'Transplantation' (Volume 97,Number 8,April 27,2014) published an open letter from Professor Delmonico along with other seven professors to Mr.Xi Jinping,President of the People's Republic of China:China's Fight Against Corruption in Organ Transplantation.The article sharply posed this concern,thus evoking great attention at home and abroad within the transplant community.To this end,we hereby state our views and declare our position with regard to the concerns mentioned in the open letter about China's organ transplant undertaking. | Jie-Fu Huang Hai-Bo Wang Shu-Sen Zheng Yong-Feng Liu Bing-Yi Shi Zhong-Yang Shen Sheng-Shou Hu Qi-Fa Ye Wu-Jun Xue Xiao-Shun He Jing-Yu Chen Feng Huo | 2015 | Chinese Medical Journal2015，，2： | 12 |
| 15 | DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments显示文摘The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses.Through a detailed analysis of the Arabidopsis thaliana genome,82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS),PI-phosphate kinases (PIPK),phospholipases (PL),inositol polyphosphate phosphatases (IPPase),inositol polyphosphate kinases (IPK),PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK,PLC,PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly,DNA chip technology was employed to study the expression patterns of various isoforms.In total,79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf,stem and flower tissues,and leaves from plants treated with various hormones (auxin,cytokinin,gibberellin,abscisic acid and brassinosteroid) or environmental factors (temperature,calcium,sodium,drought,salicylic acid and jasmonic acid).Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions.In particular,the different isoforms of each family were specifically expressed in many cases,suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses. | Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on 'Plant Molecular Physiology and Signal Transduction', 300 Fenglin Road, 200032 Shanghai, China | 2004 | Cell Research2004，14，1： | 12 |
| 16 | Discrete Variable Structural Optimization based on Multidirectional Fuzzy Genetic Algorithm显示文摘Round method is the common method for discrete variable optimization in optimal design of complex mechanical structures;however,it has some disadvantages such as poor precision,simple model and lacking of working conditions' description,etc.To solve these problems,a new model is constructed by defining parameterized fuzzy entropy,and the rationality of parameterized fuzzy entropy is verified.And a new multidirectional searching algorithm is further put forward,which takes information of actual working conditions into consideration and has a powerful local searching capability.Then this new algorithm is combined with the GA by the fuzzy clustering algorithm(FCA).With the application of FCA,the optimal solution can be effectively filtered so as to retain the diversity and the elite of the optimal solution,and avoid the structural re-analysis phenomenon between the two algorithms.The structure design of a high pressure bypass-valve body is used as an example to make a structural optimization by the proposed HGA and finite element method(FEM),respectively.The comparison result shows that the improved HGA fully considers the characteristic of discrete variable and information of working conditions,and is more suitable to the optimal problems with complex working conditions.Meanwhile,the research provides a new approach for discrete variable structure optimization problems. | LAI Yinan DAI Ye BAI Xue CHEN Dongyan | 2012 | Chinese Journal of Mechanical Engineering2012，25，2： | 12 |
| 17 | The levels, sources and reactivity of volatile organic compounds in a typical urban area of Northeast China显示文摘Air concentrations of volatile organic compounds(VOCs) were continually measured at a monitoring site in Shenyang from 20 August to 16 September 2017. The average concentrations of alkanes, alkenes, aromatics and carbonyls were 28.54, 6.30, 5.59 and9.78 ppbv, respectively. Seven sources were identified by the Positive Matrix Factorization model based on the measurement data of VOCs and CO. Vehicle exhaust contributed the most(36.15%) to the total propene-equivalent concentration of the measured VOCs,followed by combustion emission(16.92%), vegetation emission and secondary formation(14.33%), solvent usage(10.59%), petrochemical industry emission(9.89%), petrol evaporation(6.28%), and liquefied petroleum gas(LPG) usage(5.84%). Vehicle exhaust, solvent usage and combustion emission were found to be the top three VOC sources for O_3 formation potential, accounting for 34.52%, 16.55% and 11.94%, respectively. The diurnal variation of the total VOCs from each source could be well explained by their emission characteristics,e.g., the two peaks of VOC concentrations from LPG usage were in line with the cooking times for breakfast and lunch. Wind rose plots of the VOCs from each source could reveal the possible distribution of the sources around the monitoring site. The O_3 pollution episodes during the measurement period were found to be coincident with the elevation of VOCs, which was mainly due to the air parcel from the southeast direction where petrochemical industry emission was found to be dominant, suggesting that the petrochemical industry emission from the southeast was probably a significant cause of O_3 pollution in Shenyang. | Zhuobiao Ma Chengtang Liu Chenglong Zhang Pengfei Liu Can Ye Chaoyang Xue Di Zhao Jichen Sun Yiming Du Fahe Chai Yujing Mu | 2019 | Journal of Environmental Sciences2019，31，5： | 12 |
| 18 | Mitosin/CENP-F is a conserved kinetochore protein subjected to cyto plasmic dynein-mediated poleward transport显示文摘Mitosin/CENP-F is a human nuclear protein transiently associated with the outer kinetochore plate in M phase and is involved in M phase progression. LEK1 and CMF1, which are its murine and chicken orthologs, however, are implicated in muscle differentiation and reportedly not distributed at kinetochores.We therefore conducted several assays to clarify this issue. The typical centromere staining patterns were observed in mitotic cells from both human primary culture and murine, canine, and mink cell lines. A C-terminal portion of LEK1 also conferred centromere localization. Our analysis further suggests conserved kinetochore localization of mammalian mitosin orthologs. Moreover, mitosin was associated preferentially with kinetochores of unaligned chromosomes. It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein. These properties resemble those of other kinetochore proteins important for the spindle checkpoint, thus implying a role of mitosin in this checkpoint. Therefore, mitosin family may serve as multifunctional proteins involved in both mitosis and differentiation. | ZHEN YE YANG, JING GUO, NING LI, MIN QIAN, SHENG NIAN WANG, XUE LIANG ZHULaboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China | 2003 | Cell Research2003，13，4： | 11 |
| 19 | HCWCI/Carbon Steel Bimetal Liner by Liquid-Liquid Compound Lost Foam Casting显示文摘Impact, friction and corrosion from the grinding balls and the grinding medium during the mineral processing result in liner breakage. Liner, made from Hadfield steel or alloyed steel, could not have served in wet grinding environment for more than ten months. Composite liner, made from HCWCI (high Cr white cast iron) and carbon steel, has been developed successfully with liquid-liquid composing process based on LFC (lost foam casting). The microstructure of composite was analyzed with optical microscope, SEM (scanning electron microscope)/EDX energy-dispersive X-ray and XRD (X-ray diffraction). According to micrograph, the combination region of two metals was staggered like dogtooth, no mixtures occurred between two liquid metals, and its interface presented excellent metallurgical bonding state. The results of mechanical property test show that, the hardness of HRC, the fracture toughness, and the bending strength are more than 61, 16.5 J/cm2 and 1 600 MPa, respectively. Comparison between liners made from bimetal composite and alloyed steel has also been investigated in industrial hematite ball mill. The results of eight months test in wet grinding environment prove that the service life of bimetal composite liner is three times as long as that of one made from alloyed steel. | XIAO Xiao-feng1,2, YE Sheng-ping2, YIN Wei-xin3, XUE Qiong4 (1. School of Mechanical Engineering and Automation, Wuhan Textile University, Wuhan 430073, Hubei, China 2. State Key Laboratory of Material Processing and Die and Mould Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China 3. Wuhan Zhike Abrasive-Resistant Material Science and Technology Development Co Ltd, Wuhan 430073, Hubei, China 4. College of Science, Wuhan University of Technology, Wuhan 430079, Hubei, China) | 2012 | Journal of Iron and Steel Research(International)2012，19，10： | 11 |
| 20 | Usefulness of human epididymis protein 4 in predicting cytoreductive surgical outcomes for advanced ovarian tubal and peritoneal carcinoma显示文摘Objective: Human epididymis protein 4(HE4) is a promising biomarker of epithelial ovarian cancer(EOC). But its role in assessing the primary optimal debulking(OD) of EOC remains unknown. The purpose of this study is to elucidate the ability of preoperative HE4 in predicting the primary cytoreductive outcomes in advanced EOC, tubal or peritoneal carcinoma.Methods: We reviewed the records of 90 patients with advanced ovarian, tubal or peritoneal carcinoma who underwent primary cytoreduction at the Department of Obstetrics and Gynecology of Peking University People's Hospital between November 2005 and October 2010. Preoperative serum HE4 and CA125 levels were detected with EIA kit. A receiver operating characteristic(ROC) curve was used to determine the most useful HE4 cut-off value. Logistic regression analysis was performed to identify significant preoperative clinical characteristics to predict optimal primary cytoreduction.Results: OD was achieved in 47.7%(43/48) of patients. The median preoperative HE4 level for patients with OD vs. suboptimal debulking was 423 and 820 pmol/L, respectively(P<0.001). The areas under the ROC curve for HE4 and CA125 were 0.716 and 0.599, respectively(P=0.080). The most useful HE4 cut-off value was 473 pmol/L. Suboptimal cytoreduction was obtained in 66.7%(38/57) of cases with HE4 ≥473 pmol/L compared with only 27.3%(9/33) of cases with HE4 <473 pmol/L. At this threshold, the sensitivity, specificity, positive predictive value(PPV) and negative predictive value(NPV) for diagnosing suboptimal debulking were 81%, 56%, 67%, and 73%, respectively. Logistic regression analysis showed that the patients with HE4 ≥473 pmol/L were less likely to achieve OD(odds ratio =5.044, P=0.002).Conclusions: Preoperative serum HE4 may be helpful to predict whether optimal cytoreductive surgery could be obtained or whether extended cytoreduction would be needed by an interdisciplinary team. | Zhijian Tang Xiaohong Chang Xue Ye Yi Li Hongyan Cheng Heng Cui | 2015 | Chinese Journal of Cancer Research2015，27，3： | 10 |
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