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| 1 | FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis显示文摘 | Xu Zhao Ying Yang Bao-Fa Sun Yue Shi Xin Yang Wen Xiao Ya-Juan Hao Xiao-Li Ping Yu-Sheng Chen Wen-Jia Wang Kang-Xuan Jin Xing Wang Chun-Min Huang Yu Fu Xiao-Meng Ge Shu-Hui Song Hyun Seok Jeong Hiroyuki Yanagisawa Yamei Niu Gui-Fang Jia Wei Wu Wei-Min Tong Akimitsu Okamoto Chuan He Jannie M Rendtlew Danielsen Xiu-Jie Wang Yun-Gui Yang | 2014 | Cell Research2014，24，12： | 109 |
| 2 | Extensive translation of circular RNAs driven by N6-methyladenosine显示文摘广泛的 pre-mRNA 拼接背在人的 transcriptome 产生众多的圆形的 RNA (circRNAs ) 。然而,这些 circRNAs 的生物功能仍然保持大部分不清楚。这里,我们报导那 N 6-methyladenosine (m 6 一) , RNA 的最丰富的基础修正,在人的房间从 circRNAs 支持蛋白质翻译的有效开始。我们发现那一致 m 6 A 主题在 circRNAs 和单个 m 6 一个地点是足够的驾驶翻译开始。这 m 6A 驱动的翻译要求开始因素 eIF4G2 和 m 6 读者 YTHDF3,并且被 methyltransferase METTL3/14 提高,在热吃惊之上由 demethylase FTO,和 upregulated 禁止了。通过介绍的 polysome 的进一步的分析,计算预言和集体 spectrometry 揭示那 m 6 circRNAs 的 A 驱动的翻译是普遍的,与有翻译潜力的几百内长的 circRNAs。我们的学习扩展人的 transcriptome 的编码风景,并且在对环境应力的细胞的回答建议导出 circRNA 的蛋白质的一个角色。 | Yun Yang Xiaojuan Fan Miaowei Mao Xiaowei Song Ping Wu6,7 Yang Zhang Yongfeng Jin Yi Yang Ling-Ling Chen Yang Wang Catherine CL Wong Xinshu Xiao Zefeng Wang | 2017 | Cell Research2017，27，5： | 246 |
| 3 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 4 | Recent Advances on the Technologies to Increase Fertilizer Use Efficiency显示文摘To increase fertilizer use efficiency (FUE) and to minimize its negative impact on environment have been the focal points in the world for a long time. It is very important to increase FUE in China for its relatively low FUE and serious losses of nutrients. Recent advances of the technologies to increase FUE are reviewed in this article. These include site-specific and real-time nitrogen management, non-destructive quick test of the nitrogen status of plants, new types of slow release and controlled release fertilizers, site-specific nutrient management, and use of urease inhibitor and nitrification inhibitor to decrease nitrogen losses. Future outlook in technologies related to FUE improvement is also discussed. | YAN Xiang JIN Ji-yun HE Ping LIANG Ming-zao | 2008 | Agricultural Sciences in China2008，7，4： | 35 |
| 5 | The Relationship between Glucose Excursion and Cognitive Function in Aged Type 2 Diabetes Patients显示文摘Objective Evidence suggests that type 2 diabetes (T2DM) is associated with an increased risk of dementia and that glucose variability is an independent risk factor for diabetic complications.This study investigated the relationship between glucose excursion and cognitive function in aged T2DM patients.Methods A total of 248 aged T2DM patients wore a continuous glucose monitoring system (CGMS) for 3 days in order to evaluate glucose excursion,including mean amplitude of glycemic excursions (MAGE) and mean of daily difference (MODD).All subjects were evaluated with a number of accepted cognitive function tests,including themini‐mental status examination (MMSE).The relationship between MAGE and MODD and performance on these cognitive tests was assessed.Results The MAGE and MMSE score were negatively correlated,likewise with the correlation between MODD and MMSE.Liner multivariate regression analysis showed that MAGE and MODD were also negatively related to MMSE independent of age,sex,glycemic control,hypertension,smoking,or coronary heart disease history.Conclusion Glucose excursion is related to cognitive function in aged T2DM patients.Elevated glucose excursion decreased the MMSE score,which reflects general cognitive function.Thus,therapy aimed at controlling glucose excursion may be beneficial for maintaining cognitive function in aged T2DM patients. | ZHONG Yuan ZHANG Xiao Yan MIAO Ya ZHU Jie Hua YAN Hong WANG Bei Yun JIN Jun HU Ting Jun JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 34 |
| 6 | cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions显示文摘癌症房间被知道经历新陈代谢的 reprogramming 然而,支撑幸存和快速的增长 oncogenic 损害怎么在各种各样的强调条件下面协调新陈代谢的开关,尚待充分被阐明。这里,我们显示出葡萄糖或夫酸安的那剥夺,为癌症房间的二主要营养来源,被伴随由的戏剧性地激活的丝氨酸生合成小径(SSP ) 提高了 cMyc 表示。我们进一步鉴别那 cMyc 刺激了 SSP 激活由 transcriptionally 多重 SSP 酶的 upregulating 表示。而且,我们证明 cMyc 便于的 SSP 激活导致了提高的谷胱甘肽(GSH ) 生产,房间周期前进和 nucleic 酸合成,它在剥夺营养素的条件下面特别为房间幸存和增长是必要的。我们进一步揭开了那 phosphoserine 磷酸酶( PSPH ), SSP 小径的最后的限制率的酶,在 vitro 并且在 vivo 为 驾驶cMyc 的癌症前进是批评的,并且重要地, PSPH 的异常表示高度在 hepatocellular 与死亡被相关癌( HCC )病人,一般来说建议在这 调整cMyc 的酶,或 SSP 激活之间的一种潜在的原因的关系,并且癌症开发。一起拿,我们的结果表明 cMyc 的那异常表情导致提高的 SSP 激活,新陈代谢的开关的必要部分,在剥夺营养素的条件下面便于癌症前进。 | Linchong Sun Libing Song Qianfen Wan Gongwei Wu Xinghua Li Yinghui Wang Jin Wang Zhaoji Liu Xiuying Zhong Xiaoping He Shengqi Shen Xin Pan Ailing Li Yulan Wang Ping Gao Huiru Tang Huafeng Zhang | 2015 | Cell Research2015，25，4： | 33 |
| 7 | The abnormal expression of retinoic acid receptor-β,p53 and Ki67 protein,n normal,premalignant and malignant esophageal tissues显示文摘AIM: Esophageal cancer remains a significant healthiproblem worldwide. It is important to investigate alterationsin expression of retinoic acid receptor-β, p53 and Ki67proteins in esophageal carcinogenesis.METHODS: To find biomarkers for early identification ofesophageal cancer, we analyzed the retinoic acid receptor-j3,p53 protein and the proliferation marker Ki67 in surgicalspecimens of normal, mildly, and severely dysplastic andmalignant esophageal tissues by in situ hybridization ofRNA and immunohistochemistry.RESULTS: RAR-β was expressed in 94.3 %(33/35) of normalmucosae, 67.8 %(19/28) of the mild, 58.1% (18/31) of thesevere ieaions and 53.2 % (116/218) of tumor samples. RAR-β mRNA was expressed in 62.7 % (42/67), 55.1% (43/78) and29.2 % (7/24) of well, moderated and poorly differentiatedSSCs. The p53 and Ki67 proteins were 5.9 % (2/34) of thenormal mucosa. P53 and Ki67 stained positively in 10.7 % (3/28) and 21.4 % (6/28) of mild dysplasia, and 51.6 %(16/31)and 58. 1% (18/31) of severely dysplasia respectively.Samples from esophageal cancer showed no higher levers ofp53 and Ki67 expression than seen in severely dysplasticlesions. There was significant difference of RAR-β、 p53 andKi67 expression between normal mucosa and dysplatictissue or esophageal cancer.CONCLUSION: Loss of RAR-β expression and acctnulation ofp53 and Ki67 proteins may serve as biomarkers for earlyidentification of esophageal cancer in the high-riskpopulations. | Min Xu Sheng-Zu Chen,Department of Nuclear Medcine,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 10002,1,China Yu-Lan Jin Jun Fu Hong Huang Ping Qu Hai-Mei Tian ZhaoYang Liu Wei Zhang,Central Laboratory for Tumor Biology,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，2： | 34 |
| 8 | The Association of Maternal Body Composition and Dietary Intake with the Risk of Gestational Diabetes Mellitus during the Second Trimester in a Cohort of Chinese Pregnant Women显示文摘Objective To investigate the association of maternal body composition and dietary intake with the risk of gestational diabetes mellitus(GDM). Methods A total 154 GDM subjects and 981 controls were enrolled in a prospective cohort study in 11 hospitals from May 20, 2012 to December 31, 2013. Bioelectrical impedance analysis and dietary surveys were used to determine body composition and to evaluate the intake of nutrients in subjects at 21-24 weeks' gestation(WG). Logistic regression analysis was applied to explore the relationships of maternal body composition and dietary intake with the risk of GDM morbidity. Results Age, pre-pregnant body weight(BW), and body mass index(BMI) were associated with increased risk of GDM. Fat mass(FM), fat mass percentage(FMP), extracellular water(ECW), BMI, BW, energy, protein, fat, and carbohydrates at 21-24 WG were associated with an increased risk of GDM. In contrast, fat free mass(FFM), muscular mass(MM), and intracellular water(ICW) were associated with a decreased risk of GDM. Conclusion Maternal body composition and dietary intake during the second trimester of pregnancy were associated with the risk of GDM morbidity. | Xu Qing Gao Zhi Ying Li Li Ming Wang Lu Zhang Qian Teng Yue Zhao Xia Ge Sheng Jing Hong Jiang Yang Yong Tao Liu Xiao Jun Lyu Chun Jian Mao Lun Yu Xiao Ming Liu Ying Hua Kong Ai Jing Yang Xue Yan Liu Zhao Zhang Yong Wang Jin Zhang Xin Sheng Xue Chang Yong Lu Yan Ping | 2016 | Biomedical and Environmental Sciences2016，29，1： | 29 |
| 9 | Telomerase activity in gastric cancer and its clinical implications显示文摘INTRODUCTIONThedevelopmentofcarcinomaresultsfrommultipleindependentgeneticchangesthatactivateprotooncogenesorinactivatetheac... | ZHAN Wen Hua, MA Jin Ping, PENG Jun Sheng, GAO Jing Song, CAI Shi Rong, WANG Jian Ping, ZHENG Zhang Qing and WANG Lei | 1999 | World Journal of Gastroenterology1999，5，4： | 25 |
| 10 | Effect of Long-Term Application of K Fertilizer and Wheat Straw to Soil on Crop Yield and Soil K Under Different Planting Systems显示文摘Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied. The experiments on long-term application of K fertilizer and wheat straw to soil in Hebei fluvo aquic soil and Shanxi brown soil in northern China were begun in 1992. The results showed that K fertilizer and straw could improve the yields of wheat and maize with the order of NPK + St > NPK > NP + St > NP, and treatment of K fertilizer made a significant difference to NP, and the efficiency of K fertilizer in maize was higher than in wheat under rotation system of Hebei. In contrast with Shanxi, the wastage of soil potassium was a more serious issue in the rotation system in Hebei, only treatment of NPK + St showed a surplus of potassium and the others showed a wane. K fertilizer and straw could improve the content of water-soluble K, nonspecifically adsorbed K, non-exchangeable K, mineral K, and total K in contrast to NP; however, K fertilizer and straw reduce the proportion of mineral K and improve proportion of other forms of potassium in the two locating sites. Compared with the beginning of orientation, temporal variability character of soil K content and proportion showed a difference between the two soil types; furthermore, there was a decrease in the content of mineral K and total K simultaneously in the two locating sites. As a whole, the effect of K fertilizer applied to soil directly excelled to wheat straw to soil. Wheat straw to soil was an effective measure to complement potassium to increase crop yield and retard the decrease of soil K. | TAN De-shui JIN Ji-yun HUANG Shao-wen LI Shu-tian HE Ping | 2007 | Agricultural Sciences in China2007，6，2： | 25 |
| 11 | Screening and breeding of high taxol producing fungi by genome shuffling显示文摘To apply the fundamental principles of genome shuffling in breeding of taxol-producing fungi, Nodulisporium sylviform was used as starting strain in this work. The procedures of protoplast fusion and genome shuffling were studied. Three hereditarily stable strains with high taxol production were obtained by four cycles of genome shuffling. The qualitative and quantitative analysis of taxol produced was confirmed using thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and LC-MS. A high taxol producing fungus, Nodulisporium sylviform F4-26, was obtained, which produced 516.37 μg/L taxol. This value is 64.41% higher than that of the starting strain NCEU-1 and 31.52%―44.72% higher than that of the parent strains. | ZHAO Kai, PING WenXiang, ZHANG LiNa, LIU Jun, LIN Yan, JIN Tao & ZHOU DongPo Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China | 2008 | Science China(Life Sciences)2008，51，3： | 25 |
| 12 | Chinese Trauma Surgeon Association for management guidelines of vacuum sealing drainage application in abdominal surgeries-Update and systematic review显示文摘Vacuum sealing drainage (VSD) is frequently used in abdominal surgeries. However, relevant guidelines are rare. Chin ese Trauma Surge on Associati on orga nized a committee composed of 28 experts across China in July 2017, aiming to provide an evidence-based recommendation for the application of VSD in abdominal surgeries.Eleven questions regarding the use of VSD in abdominal surgeries were addressed:(1) which type of materials should be respectively chosen for the intraperitoneal cavity, retroperitoneal cavity and superficial incisions?(2) Can VSD be preventively used for a high-risk abdominal incision w让h primary suture?(3) Can VSD be used in severely contaminated/infected abdominal surgical sites?(4) Can VSD be used for temporary abdominal cavity closure under some special conditions such as severe abdominal trauma, infection, liver transplantation and intra-abdominal volume increment in abdominal compartment syndrome?(5) Can VSD be used in abdominal organ inflammation, injury, or postoperative drainage?(6) Can VSD be used in the treatment of intestinal fistula and pancreatic fistula?(7) Can VSD be used in the treatment of intra-abdominal and extra-peritoneal abscess?(8) Can VSD be used in the treatment of abdominal wall wounds, wound cavity, and defects?(9) Does VSD in crease the risk of bleeding?(10) Does VSD increase the risk of intestinal wail injury?(11) Does VSD increase the risk of peritoneal adhesion? Focusing on these questions, evidence-based recommendations were given accordingly. VSD was strongly recommended regarding the questions 2-4. Weak recommendations were made regarding questions 1 and 5-11. Proper use of VSD in abdominal surgeries can lower the risk of infection in abdominal incisions with primary suture, treat severely contaminated/infected surgical sites and facilitate temporary abdominal cavity closure. | Yang Li Pei-Yuan Li Shi-Jing Sun Yuan-Zhang Yao Zhan-Fei Li Tao Liu Fan Yang Lian-Yang Zhang Xiang-Jun Bai Jing-Shan Huo Wu-Bing He Jun Ouyang Lei Peng Ping Hu Yan-An Zhu Ping Jin Qi-Feng Shao Yan-Feng Wang Rui-Wu Dai Pei-Yang Hu Hai-Ming Chen Ge-Fei Wang Yong-Gao Wang Hong-Xu Jin Chang-Ju Zhu Qi-Yong Zhang Biao Shao Xi-Guang Sang Chang-Lin Yin | 2019 | Chinese Journal of Traumatology2019，22，1： | 26 |
| 13 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 14 | APC and K-ras gene mutation in aberrant crypt foci of human colon显示文摘AIM To study the genetic alteration in ACF andto define the possibility that ACF may be a veryearly morphological lesion with molecularchanges, and to explore the relationshipbetween ACF and colorectal adenoma evencarcinoma.METHODS DNA from 35 CRC, 15 adenomas, 34ACF and 10 normal mucus was isolated by meansof microdissection. Direct gene sequencing of K-ras gene including codon 12, 13 and 61 as well asthe mutation cluster region (MCR) of APC genewas performed.RESULTS K-ras gene mutation frequency inACF, adenoma and carcinoma was 17.6% (6/34), 13.3% (2/ 15), and 14.3% (5/ 35)respectively, showing no difference ( P > 0.05)in K-fas gene mutation among three pathologicprocedures. The K-ras gene mutation inadenoma, carcinoma and 4 ACF restricted incodon 12 (GGT→GAT), but the other 2 mutationsfrom ACF located in codon 13 (GGC→GAC). K-res gene mutation was found more frequently inolder patients and patients with polypoidcancer. No mutation in codon 61 was found in thethree tissue types. Mutation rate of APO gene inadenoma and carcinoma was 22.9% (8/35) and26.7% (4/ 15), which was higher than ACF(2.9%) (P < 0.05). APC gene mutation incarcinoma was not correlated with age ofpatients, location, size and differentiation oftumor.CONCLUSION ACF might be a very earlymorphological lesion in the tumorogenesis ofcolorectal tumor. The morphological feature andgene mutation status was different in ACF andadenoma. ACF is possibly putative'microadenoma' that might be the precursor ofadenoma. In addition, the development of asubgroup of colorectal carcinomas mightundergo a way of 'normal epithelium→ ACF→carcinomas'. | Ping Yuan~1 Meng Hong Sun~2 Jin Sheng Zhang~1 Xiong Zeng Zhu~2 Da Ren Shi~2 ~1Department of Pathology,Medical College of Fudan University,~2Department of Pathology,Cancer Hospital/Cancer Institute,Fudan University,Shanghai 200032,ChinaDr.Ping Yuan Studying Province.studying in Medical College of Fudan University,worked in Department of Pathology,Wannan Medical College,having eighteen papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 20 |
| 15 | Neuroprotective mechanisms and translational potential of therapeutic hypothermia in the treatment of ischemic stroke显示文摘Stroke is a leading cause of disability and death,yet effective treatments for acute stroke has been very limited.Thus far,tissue plasminogen activator has been the only FDA-approved drug for thrombolytic treatment of ischemic stroke patients,yet its application is only applicable to less than 4–5% of stroke patients due to the narrow therapeutic window(< 4.5 hours after the onset of stroke) and the high risk of hemorrhagic transformation.Emerging evidence from basic and clinical studies has shown that therapeutic hypothermia,also known as targeted temperature management,can be a promising therapy for patients with different types of stroke.Moreover,the success in animal models using pharmacologically induced hypothermia(PIH) has gained increasing momentum for clinical translation of hypothermic therapy.This review provides an updated overview of the mechanisms and protective effects of therapeutic hypothermia,as well as the recent development and findings behind PIH treatment.It is expected that a safe and effective hypothermic therapy has a high translational potential for clinical treatment of patients with stroke and other CNS injuries. | Jin Hwan Lee James Zhang Shan Ping Yu | 2017 | Neural Regeneration Research2017，12，3： | 19 |
| 16 | 基于轮廓特征及灰度相似度匹配的接触网绝缘子缺陷检测(英文)显示文摘目的:在图像缺陷样本少和一致性差的情况下,实现精确可靠的接触网绝缘子缺陷检测。创新点:提山一种基于瓷片轮廓特征及灰度相似度匹配的融合算法,实现了绝缘子瓷片的轮廓提取及绝缘子各瓷片的精准分离,并构建了基于瓷片间距和灰度相似度匹配的绝缘子缺陷检测模型。方法:1.采用同一个绝缘子相邻瓷片两两比较的方法进行缺陷检测,解决图像缺陷样本少和一致性差的问题。2.分两步进行检测(Fig.2):(1)基于水平梯度特征提取绝缘子各瓷片轮廓,并对瓷片轮廓内像素进行复原;(2)计算瓷片间距和灰度相似度,并与设置的阈值进行比较以区分正常绝缘子和缺陷绝缘子。结论:1.实验表明,基于轮廓特征及灰度相似度匹配的方法能够有效区分正常绝缘子和缺陷绝缘子。2.在图片数据集中,测试达到了99.50%的高召回率和91.71%的高精确度,满足了目前较高水平的接触网绝缘子缺陷检测的要求。 | Ping TAN Xu-feng LI Jin-mei XU Ji-en MA Fei-jie WANG Jin DING You-tong FANG Yong NING | 2020 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2020，21，1： | 19 |
| 17 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 18 | Efficacy and safety of Shexiang Baoxin pill(MUSKARDIA)in patients with stable coronary artery disease:a multicenter,double-blind,placebo-controlled phase IV randomized clinical trial显示文摘Background:The Shexiang Baoxin Pill(MUSKARDIA)has been used for treating coronary artery disease(CAD)and angina for more than 30 years in China.Nevertheless,methodologically sound trials on the use of MUSKARDIA in CAD patients are scarce.The aim of the study is to determine the effects of MUSKARDIA as an add-on to optimal medical therapy(OMT)in patients with stable CAD.Methods:A total of 2674 participants with stable CAD from 97 hospitals in China were randomized 1:1 to a MUSKARDIA or placebo group for 24 months.Both groups received OMT according to local tertiary hospital protocols.The primary outcome was the occurrence of a major adverse cardiovascular event(MACE),defined as a composite of cardiovascular death,non-fatal myocardial infarction(MI),or non-fatal stroke.Secondary outcomes included all-cause mortality,non-fatal MI,non-fatal stroke,hospitalization for unstable angina or heart failure,peripheral revascularization,angina stability and angina frequency.Results:In all,99.7%of the patients were treated with aspirin and 93.0%with statin.After 2 years of treatment,the occurrence of MACEs was reduced by 26.9%in the MUSKARDIA group(MUSKARDIA:1.9%vs.placebo:2.6%;odds ratio=0.80;95%confidence interval:0.45-1.07;P=0.2869).Angina frequency was significantly reduced in the MUSKARDIA group at 18 months(P=0.0362).Other secondary endpoints were similar between the two groups.The rates of adverse events were also similar between the two groups(MUSKARDIA:17.7%vs.placebo:17.4%,P=0.8785).Conclusions:As an add-on to OMT,MUSKARDIA is safe and significantly reduces angina frequency in patients with stable CAD.Moreover,the use of MUSKARDIA is associated with a trend toward reduced MACEs in patients with stable CAD.The results suggest that MUSKARDIA can be used to manage patients with CAD.Trial registration:chictr.org.cn,No.ChiCTR-TRC-12003513. | Jun-Bo Ge Wei-Hu Fan Jing-Min Zhou Hai-Ming Shi Fu-Sui Ji Yang Wu Yu-Lan Zhao Jun Qian Yuan-Zhe Jin Ying-Wu Liu Sheng-Huang Wang Sheng-Hu He Ping Yang Jie Wu Feng Lu Zi-Shan Hou | 2021 | Chinese Medical Journal2021，，2： | 17 |
| 19 | Soil Bacterial Communities Under Different Long-Term Fertilization Regimes in Three Locations Across the Black Soil Region of Northeast China显示文摘Although soil bacteria play critical roles in agro-ecosystems, the knowledge of their response to long-term fertilization across the black soil region of Northeast China is limited. In this study, we sequenced 16 S rRNA genes to assess the effects of four long-term fertilization regimes—non-fertilization(NoF), chemical fertilizer(CF), manure(M), and chemical fertilizer plus manure(CFM)—on soil properties and bacterial communities in three locations, the northern, middle, and southern parts, across the black soil region. Results showed that the influence of fertilization regimes on soil properties varied significantly among the three locations. Manure fertilization significantly increased microbial biomass carbon and relative abundance of copiotrophic bacteria. Principal component analysis(PCA)showed that the total bacterial communities were separated into three groups according to the sampling location despite long-term fertilization, and that soil pH was the most important factor in shifting bacterial communities. In addition, similar fertilization regimes resulted in different influences on bacterial community composition, and the most influential soil properties varied among the three locations. Our results highlighted that geographical separation was a more dominant factor affecting bacterial communities than fertilization, and that long-term similar fertilization regimes did not induce consistent changes in bacterial community composition in the black soil region. | HU Xiaojing LIU Junjie WEI Dan ZHU Ping CUI Xi'an ZHOU Baoku CHEN Xueli JIN Jian LIU Xiaobing WANG Guanghua | 2018 | Pedosphere2018，28，5： | 17 |
| 20 | Beclin 1 cleavage by caspase-3 inactivates autophagy and promotes apoptosis显示文摘Autophagy and apoptosis are both highly regulated biological processes that play essential roles in tissue homeostasis,development and diseases.Autophagy is also described as a mechanism of death pathways,however,the precise mechanism of how autophagy links to cell death remains to be fully understood.Beclin 1 is a dual regulator for both autophagy and apoptosis.In this study we found that Beclin 1 was a substrate of caspase-3 with two cleavage sites at positions 124 and 149,respectively.Furthermore,the autophagosome formation occurred,followed by the appearance of morphological hallmarks of apoptosis after staurosporine treatment.The cleavage products of Beclin 1 reduced autophagy and promoted apoptosis in HeLa cells and the cells in which Beclin 1 was stably knocked down by specific shRNA.In addition,the cleavage of Beclin 1 resulted in abrogating the interaction between Bcl-2 with Beclin 1,which could be blocked by z-VAD-fmk.Thus,our results suggest that the cleavage of Beclin 1 by caspase-3 may contribute to inactivate autophagy leading towards augmented apoptosis. | Yushan Zhu Lixia Zhao Lei Liu Ping Gao Weili Tian Xiaohui Wang Haijing Jin Haidong Xu Quan Chen | 2010 | Protein & Cell2010，1，5： | 17 |
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