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| 1 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 2 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 3 | Surface Oxygen Injection in Tin Disulfide Nanosheets for Efficient CO_(2) Electroreduction to Formate and Syngas显示文摘Surface chemistry modification represents a promising strategy to tailor the adsorption and activation of reaction intermediates for enhancing activity.Herein,we designed a surface oxygen-injection strategy to tune the electronic structure of SnS_(2) nanosheets,which showed effectively enhanced electrocatalytic activity and selectivity of CO_(2) reduction to formate and syngas(CO and H_(2)).The oxygen-injection SnS_(2) nanosheets exhibit a remarkable Faradaic efficiency of 91.6%for carbonaceous products with a current density of 24.1 mA cm^(−2) at−0.9 V vs RHE,including 83.2%for formate production and 16.5%for syngas with the CO/H_(2) ratio of 1:1.By operando X-ray absorption spectroscopy,we unravel the in situ surface oxygen doping into the matrix during reaction,thereby optimizing the Sn local electronic states.Operando synchrotron radiation infrared spectroscopy along with theoretical calculations further reveals that the surface oxygen doping facilitated the CO_(2) activation and enhanced the affinity for HCOO*species.This result demonstrates the potential strategy of surface oxygen injection for the rational design of advanced catalysts for CO_(2) electroreduction. | Tao Chen Tong Liu Tao Ding Beibei Pang Lan Wang Xiaokang Liu Xinyi Shen Sicong Wang Dan Wu Dong Liu Linlin Cao Qiquan Luo Wei Zhang Wenkun Zhu Tao Yao | 2021 | Nano-Micro Letters2021，13，11： | 3 |
| 4 | Specific Regulation of m^(6)A by SRSF7 Promotes the Progression of Glioblastoma显示文摘Serine/arginine-rich splicing factor 7(SRSF7),a known splicing factor,has been revealed to play oncogenic roles in multiple cancers.However,the mechanisms underlying its oncogenic roles have not been well addressed.Here,based on N6-methyladenosine(m^(6)A)co-methylation network analysis across diverse cell lines,we find that the gene expression of SRSF7 is positively correlated with glioblastoma(GBM)cell-specific m^(6)A methylation.We then indicate that SRSF7 is a novel m^(6)A regulator,which specifically facilitates the m^(6)A methylation near its binding sites on the mRNAs involved in cell proliferation and migration,through recruiting the methyltransferase complex.Moreover,SRSF7 promotes the proliferation and migration of GBM cells largely dependent on the presence of the m^(6)A methyltransferase.The two m^(6)A sites on the mRNA for PDZ-binding kinase(PBK)are regulated by SRSF7 and partially mediate the effects of SRSF7 in GBM cells through recognition by insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2).Together,our discovery reveals a novel role of SRSF7 in regulating m^(6)A and validates the presence and functional importance of temporal-and spatial-specific regulation of m^(6)A mediated by RNA-binding proteins(RBPs). | Yixian Cun Sanqi An Haiqing Zheng Jing Lan Wenfang Chen Wanjun Luo Chengguo Yao Xincheng Li Xiang Huang Xiang Sun Zehong Wu Yameng Hu Ziwen Li Shuxia Zhang Geyan Wu Meisongzhu Yang Miaoling Tang Ruyuan Yu Xinyi Liao Guicheng Gao Wei Zhao Jinkai Wang Jun Li | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 5 | Effect of Metallothionein on Cell Cycle,Apoptosis Rate and Subsets Distribution of Lymphocytes in Peripheral Blood of Dairy Cattle under Heat Stress显示文摘[Objective]This study aimed to research the effect of metallothionein on cell cycle,apoptosis rate and subsets distribution of lymphocytes in peripheral blood of dairy cows under heat stress,so as to perfect the regulative mechanism researches of MT to anti-heat stress.[Method]Twenty lactating Chinese Holstein cows were randomly divided into four groups(A,B,C and D),and injected with 0,4.0,8.0 and 12.0 mg Zn-metallothionein,respectively by intravenous route.Blood samples were collected at 1st,16th,31st,46thand 61stday,and the dynamic changes of cell cycle,apoptosis rate and subsets distribution of lymphocytes were determined.[Result]The apoptosis rate of cells in group B and C was lower than those in group A by 26.63%(P>0.05)and 24.84%(P>0.05)respectively.The number of cells in the G0/G1phage in trial groups was increased and the number of cells in the S and G2/M phages tended to decrease,but there were no significant differences(P>0.05).The number of CD3+T cell in three trial groups was greater than those in group A by 7.02%(P>0.05),5.45%(P>0.05)and 3.85%(P>0.05)respectively,while the number of CD4+T cell in trial groups was higher than those in control group by31.04%(P<0.05),35.68%(P<0.05)and 39.34%(P<0.05)respectively.The number of CD8+T cell and the levels of CD4+/CD8+in trial groups were increased observably,but significant difference(P<0.05)was observed in the levels of CD4+/CD8+between groups A and C only.It demonstrated that exogenous Zn-metallothionein can decrease apoptosis rate,improve cell cycle and regulate subsets distribution of lymphocytes in dairy cattle in a dose-dependent manner.[Conclusion]This study will provide scientific basis for safe utilization of MT in dairy industry. | Jiajie LUO Lili LI Bin ZHANG LiZhuan WU Zijun LI Ying PENG JueXin FAN XinYi LAN JinShun ZHAN Jun FANG | 2013 | Agricultural Science & Technology2013，14，10： | 1 |
| 6 | Modulatory effects of Lactiplantibacillus plantarum on chronic metabolic diseases显示文摘The increased global incidence of chronic metabolic diseases,a vital threat to human health and a burden on our healthcare systems,includes a series of clinical metabolic syndromes such as obesity,diabetes,hypertension,and dyslipidemia.One of the well-known probiotic microorganisms,Lactiplantibacillus plantarum plays an important role in promoting human health,including inhibiting the occurrence and development of a variety of chronic metabolic diseases.The present study provides an overview of the preventive and therapeutic effects of L.plantarum on diabetes,obesity,non-alcoholic fatty liver disease,kidney stone disease,and cardiovascular diseases in animal models and human clinical trials.Ingesting L.plantarum demonstrated its ability to reduce inflammatory and oxidative stress levels by regulating the production of cytokines and short-chain fatty acids(SCFAs),the activity of antioxidant enzymes,and the balance of intestinal microbial communities to alleviate the symptoms of chronic metabolic diseases.Furthermore,updated applications and technologies of L.plantarum in food and biopharmaceutical industries are also discussed.Understanding the characteristics and functions of L.plantarum will guide the development of related probiotic products and explore the modulatory benefit of L.plantarum supplementations on the prevention and treatment of multiple chronic metabolic diseases. | Lei Tian Ruixiang Zhao Xinyi Xu Zhiwei Zhou Xiaofang Xu Dongmei Luo Zhiqiang Zhou Yu Liu Ariel Kushmaro Robert S.Marks András Dinnyés Qun Sun | 2023 | Food Science and Human Wellness2023，12，4： | 1 |
| 7 | Corrosion Resistance of an Electrodeposition Zinc Coating Containing CeO2 Nanopartieles 显示文摘 | He Jianping Luo Xinyi Chen Sujing | 2002 | Materials Protection2002，37，9： | 1 |
| 8 | Manipulating nonlinear exciton polaritons in an atomically-thin semiconductor with artificial potential landscapes显示文摘Exciton polaritons in atomically thin transition-metal dichalcogenide microcavities provide a versatile platform for advancing optoelectronic devices and studying the interacting Bosonic physics at ambient conditions.Rationally engineering the favorable properties of polaritons is critically required for the rapidly growing research.Here,we demonstrate the manipulation of nonlinear polaritons with the lithographically defined potential landscapes in monolayer WS_(2)microcavities.The discretization of photoluminescence dispersions and spatially confined patterns indicate the deterministic on-site localization of polaritons by the artificial mesa cavities.Varying the trapping sizes,the polariton-reservoir interaction strength is enhanced by about six times through managing the polariton–exciton spatial overlap.Meanwhile,the coherence of trapped polaritons is significantly improved due to the spectral narrowing and tailored in a picosecond range.Therefore,our work not only offers a convenient approach to manipulating the nonlinearity and coherence of polaritons but also opens up possibilities for exploring many-body phenomena and developing novel polaritonic devices based on 2D materials. | Yuan Luo Quanbing Guo Xinyi Deng Sanjib Ghosh Qing Zhang Hongxing Xu Qihua Xiong | 2023 | Light(Science & Applications)2023，12，10： | 1 |
| 9 | CB1R-stabilized NLRP3 inflammasome drives antipsychotics cardiotoxicity显示文摘Long-term use of antipsychotics is a common cause of myocardial injury and even sudden cardiac deaths that often lead to drug withdrawn or discontinuation.Mechanisms underlying antipsychotics cardiotoxicity remain largely unknown.Herein we performed RNA sequencing and found that NLRP3 inflammasome-mediated pyroptosis contributed predominantly to multiple antipsychotics cardiotoxicity.Pyroptosis-based small-molecule compound screen identified cannabinoid receptor 1(CB1R)as an upstream regulator of the NLRP3 inflammasome.Mechanistically,antipsychotics competitively bond to the CB1R and led to CB1R translocation to the cytoplasm,where CB1R directly interacted with NLRP3 inflammasome via amino acid residues 177–209,rendering stabilization of the inflammasome.Knockout of Cb1r significantly alleviated antipsychotic-induced cardiomyocyte pyroptosis and cardiotoxicity.Multi-organ-based investigation revealed no additional toxicity of newer CB1R antagonists.In authentic human cases,the expression of CB1R and NLRP3 inflammasome positively correlated with antipsychotics-induced cardiotoxicity.These results suggest that CB1R is a potent regulator of the NLRP3 inflammsome-mediated pyroptosis and smallmolecule inhibitors targeting the CB1R/NLRP3 signaling represent attractive approaches to rescue cardiac side effects of antipsychotics. | Liliang Li Pan Gao Xinru Tang Zheng Liu Mengying Cao Ruoyu Luo Xiaoqing Li Jing Wang Xinyi Lin Chao Peng Zhihong Li Jianhua Zhang Xian Zhang Zhonglian Cao Yunzeng Zou Li Jin | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 1 |
| 10 | High performance sandwich structured Si thin film anodes with LiPON coating显示文摘 | Xinyi LUO Jialiang LANG Shasha LV Zhengcao LI | 2018 | Frontiers of Materials Science2018，12，2： | 1 |
| 11 | The transcription factor KLF14 regulates macrophage glycolysis and immune function by inhibiting HK2 in sepsis显示文摘Sepsis is a heterogeneous syndrome induced by a dysregulated host response to infection.Glycolysis plays a role in maintaining the immune function of macrophages,which is crucial for severely septic patients.However,how the pathways that link glycolysis and macrophages are regulated is still largely unknown.Here,we provide evidence to support the function of KLF14,a novel Krüppel-like transcription factor,in the regulation of glycolysis and the immune function of macrophages during sepsis.KLF14 deletion led to significantly increased mortality in lethal models of murine endotoxemia and sepsis.Mechanistically,KLF14 decreased glycolysis and the secretion of inflammatory cytokines by macrophages by inhibiting the transcription of HK2.In addition,we confirmed that the expression of KLF14 was upregulated in septic patients.Furthermore,pharmacological activation of KLF14 conferred protection against sepsis in mice.These findings uncover a key role of KLF14 in modulating the inflammatory signaling pathway and shed light on the development of KLF14-targeted therapeutics for sepsis. | Yuan Yuan Guangjian Fan Yuqi Liu Lu Liu Tong Zhang Pengfei Liu Qing Tu Xinyi Zhang Shiyuan Luo Liangfang Yao Feng Chen Jingbao Li | 2022 | Cellular & Molecular Immunology2022，19，4： | 1 |
| 12 | Ultrasmall Coordination Polymers for Alleviating ROS-Mediated Inflammatory and Realizing Neuroprotection against Parkinson's Disease显示文摘Parkinson's disease(PD)is the second most common neurodegenerative disease globally,and there is currently no effective treatment for this condition.Excessive accumulation of reactive oxygen species(ROs)and neuroinflammation are major contributors to PD pathogenesis.Herein,ultrasmall nanoscale coordination polymers(NCPs)coordinated by ferric ions and natural product curcumin(Cur)were exploited,showing efficient neuroprotection by scavenging excessive radicals and suppressing neuroinflammation.In a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced mouse PD model,such ultrasmall Fe-Cur NCPs with prolonged blood circulation and BBB traversing capability could effectively alleviate oxidative stress,mitochondrial dysfunction,and inflammatory condition in the midbrain and striatum to reduce PD symptoms.Thus,this study puts forth a unique type of therapeutics-based NCPs that could be used for safe and efficient treatment of PD with potential in clinical translation. | Guowang Cheng Xueliang Liu Yujing Liu Yao Liu Rui Ma Jingshan Luo Xinyi Zhou Zhenfeng Wu Zhuang Liu Tongkai Chen Yu Yang | 2022 | Research2022，，4： | 0 |
| 13 | Investigation on the short-term outcome and prognostic impact of predisposition,and precipitants in inpatients with chronic liver disease from Chinese AcuTe on CHronic LIver FailurE(CATCH-LIFE)cohorts显示文摘Aim:The study aimed to investigate the short-term outcomes of hospitalized patients with chronic liver disease(CLDs)and assess the prognostic impact of predisposition and precipitants,which currently remains unclear.Methods:The study included 3970 hospitalized patients with CLDs from two prospective longitudinal multicenter studies(NCT02457637 and NCT03641872)conducted in highly endemic hepatitis B virus(HBV)areas.Competing risk analysis was used to evaluate the effect of predispositions,including the etiology and severity of CLDs and precipitants;on sequential 28,90,and 365-day liver transplantation(LT)-free mortality.Results:Among all enrolled patients,76.8%of adverse outcomes(including death and LT)within one year occurred within 90 days.Compared with alcoholic etiology,the association of HBV etiology with poorer outcomes was remarkably on the 28th day(hazard ratio[HR],1.81;95%confidence interval[CI],1.07-3.06;p=0.026);however,and dimin-ished or became insignificant at 90 days and 365 days.Cirrhosis increased the adjusted risk for 365-day(HR,1.50;CI,1.13-1.99;p=0.004)LT-free mortality when compared with noncirrhosis.In patients with cirrhosis,prior decompensation(PD)independently increased the adjusted risk of 365-day LT-free mortality by 1.25-fold(p=0.021);however,it did not increase the risk for 90-day mortality.Neither the category nor the number of precipitants influenced the adjusted risk of 28 or 90-day LT-free mortality.Conclusions:The 90-day outcome should be considered a significant endpoint for evaluating the short-term prognosis of hospitalized patients with CLD.Predisposing factors,other than etiology,mainly affected the delayed(365-day)outcome.Timely effective therapy for CLD etiology,especially antiviral treatments for HBV,and post-discharge long-term surveillance monitoring in cirrhotic patients undergoing PD are suggested to enhance disease management and reduce mortality. | Yan Zhang Wenting Tan Xiaobo Wang Xin Zheng Yan Huang Beiling Li Zhongji Meng Yanhang Gao Zhiping Qian Feng Liu Xiaobo Lu Jia Shang Yubao Zheng Weituo Zhang Shan Yin Wenyi Gu Tongyu Wang Jianyi Wei Zixuan Shen Guohong Deng Yi Zhou Yixin Hou Qun Zhang Shue Xiong Jing Liu Liyuan Long Ruochan Chen Jinjun Chen Xiuhua Jiang Sen Luo Yuanyuan Chen Chang Jiang Jinming Zhao Liujuan Ji Xue Mei Jing Li Tao Li Rongjiong Zheng Xinyi Zhou Haotang Ren Yu Shi Hai Li for the CATCH‐LIFE Study Investigators of Chinese(Acute‐on)Chronic Liver Failure(CLIF)Consortium(Ch‐CLIFC) | 2023 | Portal Hypertension & Cirrhosis2023，2，3： | 0 |
| 14 | Correction to: The transcription factor KLF14 regulates macrophage glycolysis and immune function by inhibiting HK2 in sepsis显示文摘Correction to:Cellular&Molecular Immunology http://gffzzd3cc09b8251d45dfsfpncnx5c0wfk6c5v.ffgz.tsg.suse.edu.cn/10.1038/s41423-021-00806-5,published online 4 January 2022 In the version of this article initially published,two unintended errors were made during manuscript editing.The corresponding author’s name was misspelled and therefore incorrectly listed as Jingbao Li with the email address lijingbaoshanghai@163.com. | Yuan Yuan Guangjian Fan Yuqi Liu Lu Liu Tong Zhang Pengfei Liu Qing Tu Xinyi Zhang Shiyuan Luo Liangfang Yao Feng Chen Jinbao Li | 2022 | Cellular & Molecular Immunology2022，19，5： | 0 |
| 15 | Coupling effects of hydrological characteristics and nutrient load in sediments on the trophic state of reservoirs显示文摘Currently,lakes and artificial reservoirs are increasingly threatened by eutrophication,which is the result of the combined action of many natural and anthropogenic factors.In the past,the effect of nutrient load on the trophic state of water bodies has attracted much attention,while few studies have addressed the effect of hydrological characteristics.Therefore,to reveal the coupling effects of hydrological characteristics and nutrient load in sediments on the trophic state of water bodies,this study collected relevant data from 36 lakes and reservoirs across China.Pearson correlation analysis showed that trophic level index was positively and significantly correlated with nutrient load in sediments and hydraulic retention time,while it was negatively correlated with mean depth and hydraulic load.The principal component analysis showed that the nutrient load was the first major component that influenced the trophic state of water bodies,followed by the mean depth and hydraulic retention time.Eutrophication was prone to occur in water bodies with mean depth less than 7 m and hydraulic retention time greater than 14 d,and the trophic level index regression equation with hydrological characteristics and nutrient load in sediments was derived by multiple regression analysis.This study revealed that the trophic state of water bodies influenced by both nutrient load and hydrological characteristics.It provides a new idea to reduce the occurrence of eutrophication in reservoirs by using the artificial hydrological regulation capacity of reservoirs. | Fan Xinyi Yue Yihong Mei Lin Li Xiaoying Hu Zhehui Luo Jiajie Liu Liu Wang Fushun | 2021 | Acta Geochimica2021，40，4： | 0 |
| 16 | Corrigendum to “The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs” [Genes & Diseases 5 (2018) 62–74]显示文摘The authors regret having an image assembly error in Figure 5Ca,in which the image for the 'Oh dBiFP-AdRFp'group was erroneously duplicated with an overlapping image from the'36h BiFP dBIFP-AdR-simH19'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C. Haydon Hue H. Luu Lan Zhou Russell R. Reid Tong-Chuan He Xingye Wu | 2023 | Genes & Diseases2023，10，2： | 0 |
| 17 | Corrigendum to “Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes (imPODs)” [Genes & Diseases 5 (2018) 137–149]显示文摘The authors regret having an image assembly error in Figure 3A,in which the image for 'imPOD Synaptopodin DAPl stain'groupwas erroneouslyduplicatedwiththe imagefrom the'tsPOD-33C SynaptopodinDAPIstain'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C. Haydon Hue H. Luu Tong-Chuan He Hua Gan | 2023 | Genes & Diseases2023，10，2： | 0 |
| 18 | β-Cyclodextrin derived full-spectrum fluorescent carbon dots: The formation process investigation and biological applications显示文摘Carbon dots(CDs), a new building unit, have been revolutionizing the fields of biomedicine, bioimaging, and optoelectronics with their excellent physical, chemical, and biological properties. However, the difficulty of preparing excitation-dependent full-spectrum fluorescent CDs has seriously hindered their further research in fluorescence emission mechanisms and biomedicine. Here, we report full-spectrum fluorescent CDs that exhibit controlled emission changes from purple(380 nm) to red(613 nm) at room temperature by changing the excitation wavelength, and the excitation dependence was closely related to the regulation of sp2 and sp3 hybrid carbon structures by β-cyclodextrin-related groups. In addition,by regulating the content of β-cyclodextrin, the optimal quantum yields of full-spectrum fluorescent CDs were 8.97%, 8.35%, 7.90%, 9.69% and 17.4% at the excitation wavelengths of 340, 350, 390, 410 and 540 nm,respectively. Due to their excellent biocompatibility and color tunability, full-spectrum fluorescent CDs emitted bright and steady purple, blue, green, yellow, and red fluorescence in MCF-7 cells. Moreover, we optimized the imaging conditions of CDs and mitochondrial-specific dyes;and realized the mitochondrialtargeted co-localization imaging of purple, blue and green fluorescence. After that, we also explored the effect of full-spectrum fluorescent CDs in vivo fluorescence imaging through the intratumorally, subcutaneously, and caudal vein, and found that full-spectrum fluorescent CDs had good fluorescence imaging ability in vivo. | Peide Zhu Wenjing Li Yuqi Zhang Qiang Sun Yi Lin Ailin Qiu Xinyi Chen Yujin Zhou Guangfu Wu Yifan Li Zhiqiang Yu Quan Xu Dixian Luo Lulu Cai | 2023 | Chinese Chemical Letters2023，34，10： | 0 |
| 19 | Biocompatible and breathable healthcare electronics with sensing performances and photothermal antibacterial effect for motion-detecting显示文摘With the booming development of smart wearable devices,flexible multifunctional composites with high sensitivity and well health therapy have evoked great interest for next-generation healthcare electronics.However,the weak biocompatibility,low breathability,and narrow sensing range greatly hinder the development of healthcare sensors.Herein,a porous,flexible and conductive MXene/Polydimethylsiloxane/Polydopamine/Polyurethane Sponge(MXene/PDMS/PDA/PU)nanocomposite is developed as a promising motion-detecting device with good flexibility,breathability,sensing performance,photothermal therapy and antibacterial activity.Benefiting from the porous structure and biocompatible surface,this multifunctional sensor is further fabricated into a diagnostic and therapeutic system for monitoring human body motion and performing hot therapy/antibacterial treatment in the application of sports injury site.Moreover,both the wireless smart insole and cushion are constructed to gait monitoring and sit position detecting.This multifunctional hybrid sponge not only demonstrates great potential for motion monitoring sensors but also exhibits wide potential in wearable medical assistive and therapeutic systems. | Xinyi Wang Yan Tao Shaoshan Pan Xue Fang Congcong Lou Yunqi Xu Jianpeng Wu Min Sang Liang Lu Xinglong Gong Tianzhi Luo Shouhu Xuan | 2022 | npj Flexible Electronics2022，6，1： | 0 |
| 20 | Isolation,identification,and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation显示文摘To identify the cause of mass mortality of adult Macrobrachium rosenbergii in a farm in Gaoyou City,Jiangsu Province,China,a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying M.rosenbergii and identified as Aeromonas dhakensis by purification culture,biochemical characterization,and 16S rRNA and gyrB gene sequence analysis.The results of the challenge test revealed that the strain was highly pathogenic and the 50%lethal dose(LD_(50))in 72 h to M.rosenbergii was 1.54×10^(5)CFU/mL.The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes,including ascV,aexT,aer,act,lip,ompAI,gcaT,acg,and exu,supporting the strong virulence of strain DKQ-1 to M.rosenbergii.Histopathological observation of the hepatopancreas,gills,and intestines indicated that DKQ-1 injection into M.rosenbergii could cause serious tissue damage,which further supported the strong virulence of this strain.In addition,a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics,resistant to 9 kinds of antibiotics,and had intermediate resistance to spectinomycin and kanamycin.This study is the first report of A.dhakensis isolated from M.rosenbergii and provided a reference for the pathogen identification of bacterial diseases in M.rosenbergii,and for the prevention and treatment caused by A.dhakensis. | Xin PENG Haihui TU Xinyi YAO Xuan LAN Zhenxiao ZHONG Jinping LUO Qiongying TANG Shaokui YI Zhenglong XIA Guoliang YANG | 2024 | Journal of Oceanology and Limnology2024，42，2： | 0 |
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