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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | EVALUATION OF THE INTROGRESSED LINES AND SCREENING FOR ELITE GERMPLASM IN GOSSYPIUM显示文摘In this research, 155 cotton introgressed lines from interspecific hybridization have been col- lected for the purpose of evaluating the effects of enhancement of new upland cotton germplasm by interspecific hybridization, screening for elite germ- plasm and improving cotton breeding. Through iden- tification of various agricultural traits, we found that different wild cotton species had different capacities in terms of fiber quality, disease resistance, tolerance of abiotic stress, pest resistance and so on. SSR molecular marker technology has been used to de- tect the exotic genetic elements in interspecific hy- brids, and 25 SSR specific loci that can be classified into two groups were found among 15 pairs of SSR primers. Results also showed that 8 exotic germ- plasm (Gossypium barbadense, G. arboreum and G. thurberi, etc) had genetic transmission toward upland cotton. A strategy of screening elite germplasm with exotic genes based on the molecular marker-assisted breeding techniques was suggested, and 18 lines with high-quality fiber and 4 lines with resistance to Fusanrium wilt and Verticillium wilt have been ob- tained. | PANG Chaoyou DU Xiongming MA Zhiying | 2006 | Chinese Science Bulletin2006，51，3： | 4 |
| 3 | Antisense expression of Gossypium barbadense UGD6 in Arabidopsis thaliana significantly alters cell wall composition显示文摘Uridine diphosphate-glucose dehydrogenase(UGD,EC1.1.1.22 oxidizes UDP-Glc(UDP-D-glucose)to UDP-Glc A(UDP-Dglucuronate),a critical precursor of cell wall polysaccharides.Gb UGD6 from Gossypium barbadense is more highly expressed late in the elongation of cotton fibers(15 d post-anthesis(DPA))and during the stage of secondary cell wall thickening(30DPA).Subcellular localization analysis in onion epidermis revealed that fluorescently labeled Gb UGD6 protein was distributed throughout the cell membrane,as well as the nucleus and vacuoles.Examination of UGD function in Arabidopsis revealed that the antisense Gb UGD6 lines had shorter roots,deferred blossoming,compared to wild-type plants.Activities of associated enzymes were also affected by UGD reduction,and biochemical analysis of cell wall samples showed an increase in cellulose levels and a decrease in UGP-Glc A contents.The results of the present study as well as previous studies on UGD support the conclusion that UGD plays a major role in synthesizing polysaccharides synthesis in the cell wall. | Jie Han Yuxin Pan Xingfen Wang Yan Zhang Zhiying Ma | 2016 | Science China(Life Sciences)2016，59，2： | 4 |
| 4 | Two haplotype-resolved,gap-free genome assemblies for Actinidia latifolia and Actinidia chinensis shed light on the regulatory mechanisms of vitamin C and sucrose metabolism in kiwifruit显示文摘Kiwifruit is a recently domesticated horticultural fruit crop with substantial economic and nutritional value,especially because of the high content of vitamin C in its fruit.In this study,we de novo assembled two telomere-to-telomere kiwifruit genomes from Actinidia chinensis var.‘Donghong’(DH)and Actinidia latifolia‘Kuoye’(KY),with total lengths of 608327852 and 640561626 bp for 29 chromosomes,respectively.With a burst of structural variants involving inversion,translocations,and duplications within 8.39 million years,the metabolite content of DH and KY exhibited differences in saccharides,lignans,and vitamins.A regulatory ERF098 transcription factor family has expanded in KY and Actinidia eriantha,both of which have ultra-high vitamin C content.With each assembly phased into two complete haplotypes,we identified allelic variations between two sets of haplotypes,leading to protein sequence variations in 26494 and 27773 gene loci and allele-specific expression of 4687 and 12238 homozygous gene pairs.Synchronized metabolome and transcriptome changes during DH fruit development revealed the same dynamic patterns in expression levels and metabolite contents;free fatty acids and flavonols accumulated in the early stages,but sugar substances and amino acids accumulated in the late stages.The AcSWEET9b gene that exhibits allelic dominance was further identified to positively correlate with high sucrose content in fruit.Compared with wild varieties and other Actinidia species,AcSWEET9b promoters were selected in red-flesh kiwifruits that have increased fruit sucrose content,providing a possible explanation on why red-flesh kiwifruits are sweeter.Collectively,these two gap-free kiwifruit genomes provide a valuable genetic resource for investigating domestication mechanisms and genome-based breeding of kiwifruit. | Xue Han Yilin Zhang Qiong Zhang Ni Ma Xiaoying Liu Wenjing Tao Zhiying Lou Caihong Zhong Xing Wang Deng Dawei Li Hang He | 2023 | Molecular Plant2023，16，2： | 3 |
| 5 | Si-doping bone composite based on protein template- mediated assembly for enhancing bone regeneration显示文摘 | Qin YANG Yingying DU Yifan WANG Zhiying WANG Jun MA Jianglin WANG Shengmin ZHANG | 2017 | Frontiers of Materials Science2017，11，2： | 3 |
| 6 | The G-protein α subunit GhGPA positively regulates Gossypium hirsutum resistance to Verticillium dahliae via induction of SA and JA signaling pathways and ROS accumulation显示文摘Verticillium wilt, a devastating disease in cotton caused by Verticillium dahliae, reduces cotton quality and yield. Heterotrimeric GTP-binding proteins, consisting of Ga, Gb, and Gc subunits, transducers of receptor signaling, function in a wide range of biological events. However, the function of Ga proteins in the regulation of defense responses in plants is largely unexplored, except for a few reports on model species. In the present study, a cotton G-protein a-subunit-encoding gene(GhGPA) was isolated from Verticillium wilt-resistant Gossypium hirsutum(upland cotton) cv. ND601. GhGPA transcription was up-regulated under V. dahliae stress, with higher expression in tolerant than in susceptible cotton cultivars.Subcellular localization revealed GhGPA to be located in the plasma membrane. GhGPA shows high(85.0%) identity with Arabidopsis AT2 G26300(AtGPA1), and AtGPA1 gpa1-4 mutants displayed susceptibility to V. dahliae. Ectopic expression of GhGPA successfully restored the resistance of Arabidopsis gpa1-4 mutants to Verticillium wilt and made them more resistant than the wild type. Overexpression of GhGPA in Arabidopsis markedly increased the resistance and resulted in dramatic up-regulation of pathogenesis-related(PR) genes and increased in H2 O2 accumulation and salicylic acid(SA) and jasmonic acid(JA) contents. However, suppressing GhGPA expression via virus-induced gene silencing(VIGS)increased susceptibility to Verticillium wilt, down-regulated the expression of PR and marker genes in SA and JA signaling pathways, and reduced H2 O2 content. The contents of SA and JA in Arabidopsis gpa1-4 and VIGS cotton were lower than those in the wild type and empty-vector control. However,GhGPA-overexpressing Arabidopsis contained more SA and JA than the wild type when inoculated with V. dahliae. Thus, GhGPA plays a vital role in Verticillium wilt resistance by inducing SA and JA signaling pathways and regulating the production of reactive oxygen species. These findings not only broaden our knowledge about the biological role of GhGPA, but also shed light on the defense mechanisms involving GhGPA against V. dahliae in cotton. | Bin Chen Yan Zhang Jun Yang Man Zhang Qingming Ma Xingfen Wang Zhiying Ma | 2021 | The Crop Journal2021，9，4： | 3 |
| 7 | Variation of the Vaginal Microbiome During and After Pregnancy in Chinese Women显示文摘A comprehensive profiling of the vaginal microbial communities and their variability enables an accurate description of the microbiome in women.However,there is a lack of studies available on Chinese women.In the present study,the composition of the vaginal microbiota during pregnancy and the 6-week postpartum period of 454 Chinese women was characterized by sequencing the V3-V4 region of the 16S ribosomal RNA(rRNA)gene.The vaginal microbiome showed variations during pregnancy and the postpartum period based on the abortion history,hypertensive disorders,delivery mode,and maternal age.Co-variation of 22 bacterial taxa,including the Lactobacillus genus and two of its species,may account for the common characteristics of the vaginal microbiome under scenarios of different medical histories and pregnancy outcomes.In contrast,discriminant bacterial species were significantly different between women who had preterm birth(PTB)with and without premature rupture of membranes(PROM),and the community state type(CST)IV-A without any predominant Lactobacillus species in the microbiota was more prevalent during pregnancy in the PROM-PTB cases,suggesting that specific bacterial species could be considered to distinguish between different types of PTB.By providing data on Chinese women,this study will enrich the knowledge of the human microbiome and contribute to a better understanding of the association between the vaginal microbiome and reproductive health. | Xiaoai Zhang Qingzhi Zhai Jinfeng Wang Xiuling Ma Bo Xing Hang Fan Zhiying Gao Fangqing Zhao Wei Liu | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 2 |
| 8 | Study of severe and rare complications of transarterial chemoembolization (TACE) for liver cancer显示文摘 | Jinglin Xia Zhenggang Ren Shenglong Ye Dilip Sharma Zhiying Lin Yuhong Gan Yi Chen Ninglin Ge Zengchen Ma Zhiquan Wu Jia Fan Lunxiu Qin Xinda Zhou Zhaoyou Tang Binghui Yang | 2006 | European Journal of Radiology2006，，3： | 2 |
| 9 | Non-gridded library: a new approach for BAC (bacterial artificial chromosome) exploitation in hexaploid wheat (Triticum aestivum)显示文摘 | MA ZHIYING SONG WEINING PETER J SHARP | 2000 | Nucleic Acids Research2000，28，24： | 1 |
| 10 | Effect of Mo and Ni content on the mechanical properties of low carbon and alloy steel casting sample for coal-mining machinery显示文摘 | Ma Zhiying Yang Yitao | 2012 | Advanced Materials Research2012，，： | 1 |
| 11 | Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum显示文摘Gossypium herbaceum var. africanum is the only wild cotton species within the cultivated diploid G. herbaceum. As the A sub-genome donor of tetraploid cotton, it is characterized by its resistance to insects, diseases, and other adversities. We have constructed the first bacterial artificial chromosome library (BAC) for G. herbaceum var. africanum. With high quality and broad coverage, this library includes 75000 clones, with an average insert size of about 115 kb and fewer than 4% empty clones. Our library is approximately five-fold the size of the A-genome (1667 Mb) and it provides 99.3% probability for isolating genes of interest or their sequences. Using nine SSR markers that are located on five different chromosomes and linked with resistance to Verticillium wilt, seven of nine could amplify the 40 superpools and got 1-14 hits. Because of its moderate wide coverage and relative large insert size, this library will be an important genomic resource for classifying and analyzing the evolution of cotton species, as well as for isolating disease-resistance genes and control elements. | GAO HaiYan WANG XingFen LIU Fang PENG RenHai ZHANG Yan CHENG Hua MA ZhiYing WANG KunBo | 2013 | Chinese Science Bulletin2013，58，26： | 1 |
| 12 | Mining cotton fiber strength candidate genes based on transcriptome mapping显示文摘Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength. | LIU HengWei WANG XingFen PAN YuXin SHI RuiFeng ZHANG GuiYin MA ZhiYing | 2009 | Chinese Science Bulletin2009，54，24： | 1 |
| 13 | Conversion of surgically verified unresectable to resectable hepatocellular carcinoma显示文摘 | Zhaoyou Tang Yeqin Yu Zengchen Ma Rong Yang Xinda Zhou Kangda Liu Jizhen Lu Yanming Bao Zhiying Lin Binghui Yang | 1989 | Chinese Journal of Cancer Research1989，，3： | 1 |
| 14 | Activation tagging, an efficient tool for functional analysis of the rice genome显示文摘 | Shuyan Wan Jinxia Wu Zhiguo Zhang Xuehui Sun Yaci Lv Ci Gao Yingda Ning Jun Ma Yupeng Guo Qian Zhang Xia Zheng Caiying Zhang Zhiying Ma Tiegang Lu | 2009 | Plant Molecular Biology (-)2009，，1： | 1 |
| 15 | Thiophene and naphthalene-based double helix:Synthesis,structures and chirality显示文摘Both racemate and enantiomer of a novel double helix,binaphthylcyclooctaterthiophene(BN-COTh),which is a DNA-like molecule constructed by two single helices intertwined with each other via covalent bonds,have been synthesized with two building blocks,cycloocta-tetrathiophene(COTh) and cyclooctadinaphthyldithiophene(CONT) fused together via Negishi coupling reaction.Another homologue,dinaphthylcyclooctaterthiophene(DN-COTh) has been employed together as a model compound.Besides the synthetic work,BN-COTh and DN-COTh have been investigated by studying their crystal structures,spectroscopic behaviors,chiral resolution and chiral characteristics,including circular dichroism(CD) spectra and optical rotations.In addition,the novel crystal of enantiomer of(R,R,R)-BN-COTh has been explored.The enantiomer molecules packing along b-axis to form a larger and extended assembly packing due to intermolecular interactions between the enantiomer molecules and chloroform molecules in crystal. | Chunmei Zhao Zhiying Ma Chunli Li Li Xu Hua Wang | 2021 | Chinese Chemical Letters2021，32，1： | 1 |
| 16 | Non-gridded library: a new approach for BAC(bacterial artificial chromosome) exploitation in hexaploid wheat (Triticum aestivum)显示文摘 | MA Zhiying Song Weining Peter J | 2000 | Nucleic Acids Research2000，28，24： | 1 |
| 17 | Influence of maize straw amendment on soil-borne diseases of winter wheat显示文摘 | Wenchao Zhen Shutong Wang Chengyin Zhang Zhiying Ma | 2009 | Frontiers of Agriculture in China2009，，1： | 1 |
| 18 | Recommendations for the diagnosis and treatment of paroxysmal kinesigemc dyskinesia: an expert consensus in China显示文摘Paroxysmal dyskinesias are a group of neurological diseases characterized by intermittent episodes of involuntary movements with different causes.Paroxysmal kinesigenic dyskinesia(PKD)is the most common type of paroxysmal dyskinesia and can be divided into primary and secondary types based on the etiology.Clinically,PKD is characterized by recurrent and transient attacks of involuntary movements precipitated by a sudden voluntary action.The major cause of primary PKD is genetic abnormalities,and the inheritance pattern of PKD is mainly autosomal-dominant with incomplete penetrance.The proline-rich transmembrane protein 2(PRRT2)was the first identified causative gene of PKD,accounting for the majority of PKD cases worldwide.An increasing number of studies has revealed the clinical and genetic characteristics,as well as the underlying mechanisms of PKD.By seeking the views of domestic experts,we propose an expert consensus regarding the diagnosis and treatment of PKD to help establish standardized clinical evaluation and therapies for PKD.In this consensus,we review the clinical manifestations,etiology,clinical diagnostic criteria and therapeutic recommendations for PKD,and results of genetic analyses in PKD patients performed in domestic hospitals. | Li Cao Xiaojun Huang Ning Wang Zhiying Wu Cheng Zhang Weihong Gu Shuyan Cong Jianhua Ma Ling Wei Yanchun Deng Qi Fang Qi Niu Jin Wang Zhaoxia Wang You Yin Jinyong Tian Shufen Tian Hongyan Bi Hong Jiang Xiaorong Liu Yang Lu Meizhen Sun Jianjun Wu Erhe Xu Tao Chen Tao Chen Xu Chen Wei Li Shujian Li Qinghua Li Xiaonan Song Ying Tang Ping Yang Yun Yang Min Zhang Xiong Zhang Yuhu Zhang Ruxu Zhang Yi Ouyang Jintai Yu Quanzhong Hu Qing Ke Yuanrong Yao Zhe Zhao Xiuhe Zhao Guohua Zhao Furu Liang Nan Cheng Jianhong Han Rong Peng Shengdi Chen Beisha Tang | 2021 | Translational Neurodegeneration2021，10，1： | 1 |
| 19 | Ultrasound‐Targeted Microbubble Destruction Enhances Gene Expression of micro RNA ‐21 in Swine Heart via Intracoronary Delivery显示文摘 | Yangchun Liu Lang Li Qiang Su Tao Liu Zhiying Ma Huafeng Yang | 2015 | Echocardiography2015，，： | 1 |
| 20 | Effect of Mo and Ni content on theMechanical Properties of Low Carbon and Alloy Steel Casting Samplefor Coal-Mining Machinery 显示文摘 | Ma Zhiying Yang Yitao | 2012 | Advanced Materials Research2012，，： | 1 |
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