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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 3 | ShHTL7 is a non-canonical receptor for strigolactones in root parasitic weeds显示文摘 | Yao, Ruifeng Wang, Fei Ming, Zhenhua Du, Xiaoxi Chen, Li Wang, Yupei Zhang, Wenhao Deng, Haiteng Xie, Daoxin | 2017 | Cell Research2017，27，6： | 15 |
| 4 | Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation显示文摘Pancreatic ductal adenocarcinoma(PDAC)has poor prognosis due to limited therapeutic options.This study examines the roles of genome-wide association study identified PDAC-associated genes as therapeutic targets.We have identified HNF4G gene whose silencing most effectively repressed PDAC cell invasiveness.HNF4G overexpression is induced by the deficiency of transcriptional factor and tumor suppressor SMAD4.Increased HNF4G are correlated with SMAD4 deficiency in PDAC tumor samples and associated with metastasis and poor survival time in xenograft animal model and in patients with PDAC(log-rank P=0.036;HR=1.60,95%CI=1.03–2.47).We have found that Metformin suppresses HNF4G activity via AMPK-mediated phosphorylation-coupled ubiquitination degradation and inhibits in vitro invasion and in vivo metastasis of PDAC cells with SMAD4 deficiency.Furthermore,Metformin treatment significantly improve clinical outcomes and survival in patients with SMAD4-deficient PDAC(log-rank P=0.022;HR=0.31,95%CI=0.14–0.68)but not in patients with SMAD4-normal PDAC.Pathway analysis shows that HNF4G may act in PDAC through the cell-cell junction pathway.These results indicate that SMAD4 deficiency-induced overexpression of HNF4G plays a critical oncogenic role in PDAC progression and metastasis but may form a druggable target for Metformin treatment. | Chengcheng Wang Taiping Zhang Quan Liao Menghua Dai Junchao Guo Xinyu Yang Wen Tan Dongxin Lin Chen Wu Yupei Zhao | 2021 | Protein & Cell2021，12，2： | 5 |
| 5 | The role of histone methylation in the development of digestive cancers:a potential direction for cancer management显示文摘Digestive cancers are the leading cause of cancer-related death worldwide and have high risks of morbidity and mortality.Histone methylation,which is mediated mainly by lysine methyltransferases,lysine demethylases,and protein arginine methyltransferases,has emerged as an essential mechanism regulating pathological processes in digestive cancers.Under certain conditions,aberrant expression of these modifiers leads to abnormal histone methylation or demethylation in the corresponding cancer-related genes,which contributes to different processes and phenotypes,such as carcinogenesis,proliferation,metabolic reprogramming,epithelial–mesenchymal transition,invasion,and migration,during digestive cancer development.In this review,we focus on the association between histone methylation regulation and the development of digestive cancers,including gastric cancer,liver cancer,pancreatic cancer,and colorectal cancer,as well as on its clinical application prospects,aiming to provide a new perspective on the management of digestive cancers. | Yuan Chen Bo Ren Jinshou Yang Huanyu Wang Gang Yang Ruiyuan Xu Lei You Yupei Zhao | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 6 | Evolution Process of Urban Spatial Pattern in Hubei Province Based on DMSP/OLS Nighttime Light Data显示文摘This paper intends to investigate the urban spatial patterns of Hubei Province and its evolution from three different perspectives: urban nodes, urban connections and urban clusters. The research adopts nighttime light imagery of cities in Hubei Province, the viewpoint of ′point-axis-area′ in the ′point-axis system′ theory, and employs light index model, gravity model and social network analysis. The findings are as follows: 1) In terms of urban nodes, the urbanization process of Hubei has been carried out mainly on the basis of external expansion rather than internal increasing. The polarization trend of urban connection network is strengthening. 2) As for urban connections, the estimation of urban connections using light index model is capable of containing various actual flow, and the connections are getting increasingly closer. 3) In regard to urban groups, seven urban groups of varying sizes have formed. On that basis, three stable and relatively independent urban groups as the centers, namely Wuchang, Yichang and Xiangyang emerge as well. But the structures of ′Wuhan Metropolitan Area′, ′Yichang-Jingzhou-Jingmen City Group′ and ′Xiangyang-Shiyen-Suizhou City Group′, which are defined by local development strategy in Hubei Province, are different from the above three urban groups. | ZHENG Wensheng RUN Jiying ZHUO Rongrong JIANG Yupei WANG Xiaofang | 2016 | Chinese Geographical Science2016，26，3： | 3 |
| 7 | Mechanisms of obesity-and diabetes mellitus-related pancreatic carcinogenesis:a comprehensive and systematic显示文摘Research on obesity-and diabetes mellitus(DM)-related carcinogenesis has expanded exponentially since these two diseases were recognized as important risk factors for cancers.The growing interest in this area is prominently actuated by the increasing obesity and DM prevalence,which is partially responsible for the slight but constant increase in pancreatic cancer(PC)occurrence.PC is a highly lethal malignancy characterized by its insidious symptoms,delayed diagnosis,and devastating prognosis.The intricate process of obesity and DM promoting pancreatic carcinogenesis involves their local impact on the pancreas and concurrent wholebody systemic changes that are suitable for cancer initiation.The main mechanisms involved in this process include the excessive accumulation of various nutrients and metabolites promoting carcinogenesis directly while also aggravating mutagenic and carcinogenic metabolic disorders by affecting multiple pathways.Detrimental alterations in gastrointestinal and sex hormone levels and microbiome dysfunction further compromise immunometabolic regulation and contribute to the establishment of an immunosuppressive tumor microenvironment(TME)for carcinogenesis,which can be exacerbated by several crucial pathophysiological processes and TME components,such as autophagy,endoplasmic reticulum stress,oxidative stress,epithelialmesenchymal transition,and exosome secretion.This review provides a comprehensive and critical analysis of the immunometabolic mechanisms of obesity-and DM-related pancreatic carcinogenesis and dissects how metabolic disorders impair anticancer immunity and influence pathophysiological processes to favor cancer initiation. | Rexiati Ruze Jianlu Song Xinpeng Yin Yuan Chen Ruiyuan Xu Chengcheng Wang Yupei Zhao | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 8 | Dielectr ic barr ier discharge plasma-assisted modification of g-C_(3)N_(4)/Ag_(2)O/TiO_(2)-NRs composite enhanced photoelectrocatalytic activity显示文摘Dielectric barrier discharge(DBD)plasma applied as surface treatment technology was employed for the modification of Ag_(2)O and graphitic carbon nitride(g-C_(3)N_(4))powders.Subsequently,the pretreated powders were sequentially loaded onto TiO_(2)nanorods(TiO_(2)-NRs)via electro-deposition,followed by calcination at N_(2)atmosphere.The results indicated that at the optimal plasma discharge time of 5 min for modification of g-C_(3)N_(4)and Ag_(2)O,photocurrent density of ternary composite was 6 times to bare TiO_(2)-NRs under UV-visible light irradiation.Phenol was degraded by using DBD plasma-modified g-C_(3)N_(4)/Ag_(2)O/TiO_(2)-NRs electrode to analyze the photoelectrocatalytic performance.The removal rate of phenol for g-C_(3)N_(4)-5/Ag_(2)O-5/TiO_(2)-NRs electrode was about 3.07 times to that for TiO_(2)-NRs electrode.During active species scavengers'analysis,superoxide radicals and hydroxyl radicals were the main oxidation active species for pollutants degradation.A possible electron-hole separation and transfer mechanism of ternary composite with high photoelectrocatalytic performance was proposed. | Yupei Long Chenchen Yuan Xiaomin Wang Dongyan Jin Hong Zhou Qiongyin Wang Chenyang Lu Yuqi Chen Yanqing Cong Qi Wang Yi Zhang | 2021 | Journal of Environmental Sciences2021，33，6： | 2 |
| 9 | Versatile.design and synthesis of mesoporous sulfonic acid catalysts显示文摘Mesoporous sulfonic acid catalysts(MSAC) are widely used in acid-catalyzed reactions, including biomass conversions with plenty of polar solvents and precursors. The catalytic efficiency of MSAC is greatly affected by the microenvironment around the sulfonic acid sites. In this review, the progress on modification of microenvironment of MSAC is reviewed over the past decade. Hydrophobic modification allows MSAC prevent the adhesion of water molecules onto sulfonic acid sites, to abate the risk of reduced acid strength and catalytic efficiency. In comparison, hydrophilic properties can bring positive effect on acidcatalyzed reactions with the aid of hydrophilic interaction between polar functional groups on MSAC and hydrophilic groups of specific substrates. Amphiphilic MSAC with tunable wettability for specific substrates and solvents tend to improve the efficiency in certain reactions with mixed solvents or reactants of different polarity, especially for biphasic systems of immiscible liquids. Furthermore, much attention has been attracted on modification of surface to simulate the microenvironment of homogeneous solvents and enzyme biocatalysts in recent research. New trends of this field are also highlighted. | Peng Wang Yupei Zhao Jian Liu | 2018 | Science Bulletin2018，63，4： | 2 |
| 10 | Selective potassium uptake via biocompatible zeolite–polymer hybrid microbeads as promising binders for hyperkalemia显示文摘Patients with chronic kidney disease are at high risk of hyperkalemia that is associated with various lifethreatening complications.Treatments primarily rely on orally administered potassium binding agents,along with low curative effects and various side effects.Herein,direct serum potassium uptake was realized via zeolite–heparin-mimicking-polymer hybrid microbeads.The preparation process involved the synthesis of the heparin-mimicking polymer via the in situ cross-linking polymerization of acrylic acid and N-vinylpyrrolidone in polyethersulfone solution,the fabrication of microbeads via zeolite-mixing,electro-spraying and phase-inversion,and the subsequent aqueous-phase modifications based on ion-exchange and metal-leaching.An ultra-high(about 88%)amount of zeolite could be incorporated and well locked inside the polymer matrix.Potassium uptake capability was verified in water,normal saline and human serum,showing high selectivity and fast adsorption.The microbeads exhibited satisfying blood compatibility,negligible hemolysis ratio,prolonged clotting time,inhibited contact activation,and enhanced antifouling property toward serum proteins and cells.The proposed approach toward zeolite–heparin-mimicking-polymer hybrid microbeads provided a cheap,efficient and safe treatment protocol of hyperkalemia for the high-risk patients. | Zhoujun Wang Wei Sun Zhiwei Wei Jianxu Bao Xin Song Yupei Li Haifeng Ji Jue Zhang Chao He Baihai Su Weifeng Zhao Changsheng Zhao | 2021 | Bioactive Materials2021，6，2： | 2 |
| 11 | Polybenzimidazole functionalized electrolyte with Li-wetting and self-fluorination functionalities for practical Li metal batteries显示文摘Rough Li plating,low ionic conductivity,and low thermal stability of conventional electrolytes post-primary challenges for achieving reliable high-capacity rechargeable lithium batteries,for which lithiummetal is frequently proposed as themost promising anode material.Conventional low-polarity commercial polypropylene/polyethylene separators fail to support the application of high-energy-density Li anodes due to their rigid physicochemical properties and the high reactivity of Li metal,leading to fatal dendrite formation and vigorous exothermic reaction with electrolytes.Herein,we develop a Li-wetting,flame-retardant binary polymer electrolyte by functionalizing poly(vinylidene fluoride)(PVDF)separators with nonflammable polybenzimidazole(PBI)to build safe room-temperature solid-state electrolyte membranes.A dendrite-free LiFePO4 cell with the solid polymer electrolyte(SPE)delivers a discharge capacity of 127 mAh g^(-1) at 25℃ with a capacity retention of 87.5%after 500 cycles at 0.5℃(0.15 mA cm^(-2)).Phase-field simulations and density functional theory calculations demonstrate that the negatively charged benzimidazole chains of PBI own superior affinity to lithium bis(trifluoromethanesulfonyl)imide(LiTFSI),and shares overlapping electron density with Li anode,giving rise to accelerated Li^(+)conduction at room temperature and uniform Li electrodeposition at the electrolyte/Li metal interface.The SPE is also flame-retardant since heat-resistant polytetrafluoroethylene and a dense,heat-blocking graphitized carbon layer are formed in intense heat throughdehydrogenation/fluorination of PVDF under the catalysis of Lewis base imidazole rings and the decomposition of benzimidazole rings in PBI.No such fire-resistant mechanism is ever reported in conventional electrolytes. | Dongjiang Chen Yuanpeng Liu Chuan Xia Yupei Han Qingwei Sun Xuchang Wang Wei Chen Xian Jian Weiqiang Lv Jianyi Ma Weidong He | 2022 | InfoMat2022，4，5： | 1 |
| 12 | Preparation of a novel sulfonated carbon catalyst for the etherification of isopentene with methanol to produce tert -amyl methyl ether显示文摘 | Yu Zhao Hezhi Wang Yupei Zhao Jianyi Shen | 2010 | Catalysis Communications2010，，9： | 1 |
| 13 | Targeting the Neddylation Pathway to Suppress the Growth of Prostate Cancer Cells: Therapeutic Implication for the Men’s Cancer显示文摘 | Xiaofang Wang Lihui Li Yupei Liang Chunjie Li Hu Zhao Dingwei Ye Menghong Sun Lak Shin Jeong Yan Feng Shen Fu Lijun Jia Xiaomao Guo Zhen Chen | 2014 | BioMed Research International2014，，： | 1 |
| 14 | Phase separation of the nuclear pore complex facilitates selective nuclear transport to regulate plant defense against pathogen and pest invasion显示文摘The nuclear pore complex(NPC),the sole exchange channel between the nucleus and cytoplasm,is composed of several subcomplexes,among which the central barrier determines the permeability/selectivity of the NPC to dominate the nucleocytoplasmic trafficking essential for many important signaling events in yeast and mammals.How plant NPC central barrier controls selective transport is a crucial question remaining to be elucidated.In this study,we uncovered that phase separation of the central barrier is critical for the permeability and selectivity of plant NPC in the regulation of various biotic stresses.Phenotypic assays of nup62 mutants and complementary lines showed that NUP62 positively regulates plant defense against Botrytis cinerea,one of the world’s most disastrous plant pathogens.Furthermore,in vivo imaging and in vitro biochemical evidence revealed that plant NPC central barrier undergoes phase separation to regulate selective nucleocytoplasmic transport of immune regulators,as exemplified by MPK3,essential for plant resistance to B.cinerea.Moreover,genetic analysis demonstrated that NPC phase separation plays an important role in plant defense against fungal and bacterial infection as well as insect attack.These findings reveal that phase separation of the NPC central barrier serves as an important mechanism to mediate nucleocytoplasmic transport of immune regulators and activate plant defense against a broad range of biotic stresses. | Jiaojiao Wang Gaofeng Pei Yupei Wang Dewei Wu Xiaokang Liu Gaoming Li Jianfang He Xiaolin Zhang Xiaoyi Shan Pilong Li Daoxin Xie | 2023 | Molecular Plant2023，16，6： | 1 |
| 15 | Guidelines for the diagnosis and treatment of acute pancreatitis in China (2021)显示文摘Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China. | Fei Li Shouwang Cai Feng Cao Rufu Chen Deliang Fu Chunlin Ge Chunyi Hao Jihui Hao Heguang Huang Zhixiang Jian Gang Jin Ang Li Haimin Li Shengping Li Weiqin Li Yixiong Li Tingbo Liang Xubao Liu Wenhui Lou Yi Miao Yiping Mou Chenghong Peng Renyi Qin Chenghao Shao Bei Sun Guang Tan Xiaodong Tian Huaizhi Wang Lei Wang Wei Wang Weilin Wang Junmin Wei Heshui Wu Wenming Wu Zheng Wu Changqing Yan Yinmo Yang Xiaoyu Yin Xianjun Yu Chunhui Yuan Taiping Zhang Yupei Zhao on behalf of the Chinese Pancreatic Surgery Association | 2021 | Journal of Pancreatology2021，4，2： | 1 |
| 16 | Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity显示文摘Pancreatic cancer is one of the most lethal human malignancies, partly because of its propensity for metastasis. However, highly metastatic human pancreatic cancer cell lines suitable for studies of metastasis are currently lacking. Here we established two highly metastatic human pancreatic cancer cell lines, MIA PaCa-2 In8 and Panc-1 In8, by Matrigel induction assay. The cell lines were further characterized both in vitro and in vivo. MIA PaCa-2 In8 and Panc-1 In8 cells demonstrated increased migration and invasion compared with their respective parental cells. Following injection into nude mice, MIA PaCa-2 In8 and Panc-1 In8 cells resulted in more pulmonary metastases compared with the parental cells. Furthermore, analyses of m RNA, long non-coding RNA, micro RNA, and methylation profiling revealed that these factors were aberrantly regulated in the highly metastatic cells,indicating that they probably affected metastasis. We thus established and characterized two highly metastatic human pancreatic cell lines that could be used as valuable tools for future investigations into the pathogenesis, metastasis, and potential treatment of human pancreatic cancer. | Gang Yang Huanyu Wang Mengyu Feng Lei You Lianfang Zheng Taiping Zhang Lin Cong Yupei Zhao | 2019 | Science China(Life Sciences)2019，62，6： | 1 |
| 17 | Author Correction: 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘In the initial published version of this article, there was a mistake in the P value for the correlation between gene-expression changes and 5 hmC changes in tumors. The correct P value should be same as the P value shown in Fig. S6A: 9.8?×?10?6 (mistakenly shown as “9.8?×?106” in the main text). This correction does not affect the description of results or the conclusions of this study, since the range of P value is between 0 and 1. | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2019 | Cell Research2019，29，7： | 1 |
| 18 | Effects of extracts from soothing-liver and invigorating-spleen formulas on the injury induced by oxidative stress in the hepatocytes of rats with non-alcoholic fatty liver disease induced by high-fat diet显示文摘OBJECTIVE:To investigate effects of the extracts from soothing-liver and invigorating-spleen formulas on the injury due to oxidative stress,mediated by the Nuclear factor-like 2(Nrf2)-Antioxidant response element(ARE) pathway,in the hepatocytes of rats with non-alcoholic fatty liver disease(NAFLD) induced by high-fat diet.METHODS:Soothing-liver and invigorating-spleen formula mixtures were prepared for five groups:normal,model,soothing-liver formula group(SLG),invigorating-spleen formula group(ISG),integrated formula group(IG).The rat model of NAFLD was induced by feeding rats a high-fat diet(HFD).After 16 weeks,the hepatic tissue was examined following Haematoxylin-Eosin(H&E) staining and with Transmission electron microscopy(TEM).Levels of hepatic lipids,serum lipids and hepatic functions were measured using a biochemical analyser.Hepatocytes were isolated from the livers of rats and were identified by cellar immunohistochemistry,cellular immunofluorescence and flow cytometry.The expression levels of Nrf2,Kelch-like epichlorohydrin-associated protein 1(Keap-1),haeme oxygenase-1(HO-1) and NAD(P)H:quinone oxidoreductase 1(NQO1) m RNAs were assessed by real-time fluorescence quantitative PCR.Nrf2,Keap-1,HO-1 and NQO1 proteins were measured by Western blotting.Finally,the levels of oxidative stress factors Superoxide Dismutase(SOD),malonaldehyde(MDA) and Glutathione peroxidase(GSH-Px) in hepatocytes were measured by WST-1,TBA and colorimetry.RESULTS:The H & E and TEM results showed that the NAFLD model rats successfully reproduced typical pathogenetic and histopathological features.The liver function and levels of hepatic lipids and serum lipids from the model rats were dramatically increased.Compared with the model group,the levels of hepatic lipids,serum lipids and hepatic function in the treatment groups were ameliorated to different degrees.The yields of purified hepatocytes in each rat were 4-5 × 108.The viability of the isolated hepatocytes was higher than 95%,with a purity over 93.2%.Cellular immunohistochemistry analysis showed that the hepatocytes were brown,while in the cellular immunofluorescence analysis,the hepatocytes showed green fluorescence.The expression levels of Nrf2,Keap-1,HO-1 and NQO1 m RNA and protein in the hepatocytes were significantly higher in the model group than in the normal group(P < 0.05,P < 0.01).Compared with the model group,the expression of Nrf2,Keap-1,HO-1 and NQO-1 m RNAs and proteins in all treatment groups increased,especially in the IG(P < 0.01).CONCLUSION:The extracts from soothing-liver and invigorating-spleen formulas may protect the liver against the injury induced by oxidative stress in hepatocytes by influencing the Nrf2-ARE pathway,which may be the mechanism having the potential for prevention and treatment of NAFLD. | Tu Guifang Gong Xiangwen Yang Qinhe Li Yuanyuan Wang Guanlong Liang Yinji Zhang Yupei Yan Haizhen Lin Chunmei Zhang Jinwen | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 1 |
| 19 | Magnesium cobalt silicate materials for reversible magnesium ion storage显示文摘 | Yupei Zheng Yanna NuLi Qiang Chen Ying Wang Jun Yang Jiulin Wang | 2012 | Electrochimica Acta2012，，： | 1 |
| 20 | 3-72 Early Embryonic Exposure of Ionizing Radiation Disrupts Zebrafish Pigmentation显示文摘Mitochondrial F1F0-ATPase has been shown to modulate pigmentation negatively in zebrafish[1;2]. Both of these works hypothesize that the alkalization of cellular endomembrane system, as induced by inhibition of mitochondrial ATPase-mediated transport of H+ ions, facilitates trafficking of Tyr to the melanosomes that enhance zebrafish pigmentation.Wang[3] et al. further identified that Beta adrenergic receptors ( -ARs) regulate zebrafish pigmentation partly by modulating F1F0-ATPase. These studies suggest the important role of mitochondrial F1F0-ATPase in zebrafish pigmentation, but whether IR could affect F1F0-ATPase function has not been studied. | Wang Yupei Zhou Xin Zhang Hong | 2015 | IMP & HIRFL Annual Report2015，，1： | 0 |
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