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| 1 | Ridge subduction and porphyry copper-gold mineralization:An overview显示文摘Many large porphyry Cu-Au deposits are connected to adakitic rocks known to be closely associated with ridge subduction. For example, there are several subducting ridges along the east Pacific margin, e.g., in Chile, Peru, and South America, most of which are associated with large porphyry Cu-Au deposits. In contrast, there are much fewer ridge subductions on the west Pacific margin and porphyry Cu-Au deposits are much less there, both in terms of tonnage and the number of deposits. Given that Cu and Au are moderately incompatible elements, oceanic crust has much higher Cu-Au concentrations than the mantle and the continental crust, and thus slab melts with their diagnostic adakitic chemistry have systematically higher Cu and Au, which is favorable for mineralization. Considering the geotherm of subducting slabs in the Phanerozoic, ridge subduction is the most favorable tectonic setting for this. Therefore, slab melting is the likely link in the spatial association between ridge subduction and Cu-Au deposits. Geochemical signatures of slab melting and hence maybe ridge subduction in less eroded regions in eastern China, the central Asian orogenic belt etc. may indicate important exploration targets for large porphyry Cu-Au deposits. | SUN WeiDong LING MingXing YANG XiaoYong FAN WeiMing DING Xing LIANG HuaYing | 2010 | Science China Earth Sciences2010，53，4： | 97 |
| 2 | Exogenous plant MIR168a specifically targets mammalian LDLRAPI: evidence of cross-kingdom regulation by microRNA显示文摘 | Lin Zhang Dongxia Hou Xi Chen Donghai Li Lingyun Zhu Yuj ing Zhang Jing Li Zhen Bian Xiangying Liang Xing Cai Yuan Yin Cheng Wang Tianfu Zhang Dihan Zhu Dianmu Zhang Jie Xu Qun Chen Yi Ba Jing Liu Qiang Wang Jianqun Chen Jin Wang Meng Wang Qipeng Zhang Junfeng Zhang Ke Zen Chen-Yu Zhang | 2012 | Cell Research2012，22，1： | 155 |
| 3 | Tumor-secreted miR-214 induces regulatory T cells: a major link between immune evasion and tumor growth显示文摘 | Yuan Yin Xing Cai Xi Chen Hongwei Liang Yujing Zhang Jing Li Zuoyun Wang Xiulan Chen Wen Zhang Seiji Yokoyama Cheng Wang Liang Li Limin Li Dongxia Hou Lei Dong Tao Xu Takachika Hiroi Fuquan Yang Hongbin Ji Junfeng Zhang Ke Zen Chen-Yu Zhang | 2014 | Cell Research2014，24，10： | 33 |
| 4 | Hydroxysafflor yellow A improves learning and memory in a rat model of vascular dementia by increasing VEGF and NR1 in the hippocampus显示文摘Hydroxysafflor yellow A(HSYA) has angiogenesisregulating and neuro-protective effects, but its effects on vascular dementia(VaD) are unknown. In this study, 30 adult Sprague-Dawley rats were randomly allocated to five groups: normal, sham-operation, VaD alone(bilateral carotid artery occlusion), VaD plus saline(control), and VaD plus HSYA. One week after operation, the HSYA group received one daily tail-vein injection of 0.6 mg/100 g HSYA for two weeks. Five weeks after operation, the spatial memory of all five groups was evaluated by the water maze task, and synaptic plasticity in the hippocampus was assessed by the long-term potentiation(LTP) method. Vascular endothelial growth factor(VEGF) and N-methyl-Daspartic acid receptor 1(NR1) expression in the hippocampus was detected via Western blot. We found that, compared with the group with VaD alone, the group with HSYA had a reduced escape latency in the water maze(P <0.05), and the LTP at CA3-CA1 synapses in the hippocampus was enhanced(P <0.05). Western blot in the late-phase VaD group showed slight up-regulation of VEGF and downregulation of NR1 in the hippocampus, while HSYA significantly up-regulated both VEGF and NR1. Theseresults suggested that HSYA promotes angiogenesis and increases synaptic plasticity, thus improving spatial learning and memory in the rat model of VaD. | Nan Zhang Mengya Xing Yiyi Wang Hao Liang Zhuo Yang Fudong Shi Yan Cheng | 2014 | Neuroscience Bulletin2014，30，3： | 30 |
| 5 | A review of lithium-ion battery safety concerns:The issues,strategies,and testing standards显示文摘Efficient and reliable energy storage systems are crucial for our modern society.Lithium-ion batteries(LIBs)with excellent performance are widely used in portable electronics and electric vehicles(EVs),but frequent fires and explosions limit their further and more widespread applications.This review summarizes aspects of LIB safety and discusses the related issues,strategies,and testing standards.Specifically,it begins with a brief introduction to LIB working principles and cell structures,and then provides an overview of the notorious thermal runaway,with an emphasis on the effects of mechanical,electrical,and thermal abuse.The following sections examine strategies for improving cell safety,including approaches through cell chemistry,cooling,and balancing,afterwards describing current safety standards and corresponding tests.The review concludes with insights into potential future developments and the prospects for safer LIBs. | Yuqing Chen Yuqiong Kang Yun Zhao Li Wang Jilei Liu Yanxi Li Zheng Liang Xiangming He Xing Li Naser Tavajohi Baohua Li | 2021 | Journal of Energy Chemistry2021，30，8： | 29 |
| 6 | Treatment of chronic prostatitis in Chinese men显示文摘The aim of this study is to assess the status of treatment of chronic prostatitis(CP)in Chinese men.A population-based cross-sectional survey was performed,in which 15000 men aged between 15 and 60 years were randomly selected to receive a questionnaire designed to assess National Institutes of Health Chronic Prostatitis Symptoms Index(NIH-CPSI)status,therapeutic efficacy and 28 other items.A total of 12743 men(84.95%)completed the questionnaire,of whom 1071(8.4%)were identified as having prostatitis-like symptoms and 517(4.5%)were diagnosed with CP according to NIH-CPSI criteria and prostatitis-like symptomatology.Of the CP patients,372(65%)underwent long-term routine treatment 12 times per year.Additionally,217(72.8%)patients received antibiotic therapy and 215(79.3%)men showed therapeutic effects.The treatment cost USD 1151(8059 yuan)per person per year on average.Most CP patients received routine treatment,in most cases with antibiotics.Treatment was costly and most CP patients were not satisfied with its effectiveness.Antibacterial treatment might have been effective primarily in patients with bacterial disease. | Chao-Zhao Liang Hong-Jun Li Zhi-Ping Wang Jun-Ping Xing Wei-Lie Hu Tao-Fu Zhang Wei-Wei Ge Zong-Yao Hao Xian-Sheng Zhang Jun Zhou Yu Li Zheng-Xing Zhou Zhi-Guo Tang | 2009 | Asian Journal of Andrology2009，11，2： | 27 |
| 7 | Possible early Neoproterozoic magmatism associated with slab window in the Pingshui segment of the Jiangshan-Shaoxing suture zone: Evidence from zircon LA-ICP-MS U-Pb geochronology and geochemistry显示文摘We report here geochemical data, U-Pb zircon ages, and Hf isotopes for the high-Mg diorites (HMDs), Nb-enriched basaltic porphyrys (NEBPs) and plagiogranites (PLAGs) in the Pingshui segment of the Jiangshan-Shaoxing suture zone. The HMDs are characterized by high Mg# (>60), high Na and LREE contents, depletion of HREE and HFSE, and pronounced positive εNd(t) values of 7.0 to 7.7, similar to some adakitic high-Mg andesites. The NEBPs are relatively Na-rich (Na2O/K2O>6) and display high abundances of P2O5 (~1.00%), TiO2 (~3.08%) and HFSE (e.g., Nb=9.53-10.27 ppm). Their Nd isotopic compositions (εNd(t)=6.8-8.0) are comparable to those of the HMDs. The PLAGs are metaluminous (A/CNK=0.84-0.89) and sodic (Na2O/K2O>10). Their depletion in HFSE (e.g., Nb, Ta) is consistent with 'SSZ-type' plagiogranite. Zircon LA-ICP-MS U-Pb dating yields an age of 932±7 Ma for the HMD, 916±6 Ma for the NEBP, and 902±5 Ma for the PLAG, respectively, indicating that they were products of early Neoproterozoic magmatism. The PLAGs exhibit relatively high zircon Hf isotopes and positive εHf(t) values of 11.0 to 16.2, consistent with their Nd isotopic data (εNd(t)=7.5-8.4). Such features are similar to those of oceanic plagiogranites in ophiolites and distinct from those of crust-derived granites. The PLAGs were most likely derived from partial melting of subducted oceanic crust in an active continental margin. Considering these results in the context of the regional geology, we suggest that a slab window in the subducting oceanic crust between the Yangtze Block and Cathaysia Block was possibly the principal cause for the unique arc magmatism in the area. The upwelling asthenosphere below the slab window may have provided significant thermodynamic conditions. | CHEN ZhiHong GUO KunYi DONG YongGuan CHEN Rong LI LongMing LIANG YiHong LI ChunHai YU XiMing ZHAO Ling XING GuangFu | 2009 | Science China Earth Sciences2009，52，7： | 26 |
| 8 | A Genome-Wide Transcription Analysis Reveals a Close Correlation of Promoter INDEL Polymorphism and Heterotic Gene Expression in Rice Hybrids显示文摘杂种优势,或混合活力,指二个生来的变化的混合子孙在展出提高的生长或农学的性能的现象。尽管研究的世纪长的历史关于杂种优势的基因基础产生了几个假设,位于杂种优势和 heterotic 基因表示下面的分子的机制留下逃犯。这里,我们在米饭报导二个 heterotic 十字的染色体宽的基因表示分析,利用它的充分定序的染色体。约 79% 基因是从 heterotic 十字的二个集合在幼苗射击表示的差别,包括许多抄写因素基因,并且展出了基因行动的多重模式。在生来的父母之间的 ortholog 基因的通常认为的倡导者区域的比较揭示了广泛的顺序变化,特别地小的插入 / 删除(INDEL ) ,许多哪个在通常认为的 cis 规章的元素的形成 / 混乱的结果。一起,这些结果建议那在抄写因素和多态的倡导者的表示之间的组合相互影响在混血儿的 cis 规章的元素是位于 heterotic 基因行动下面的一嘴巧的分子的机制并且这样在米饭的杂种优势。 | Hui-Yong Zhang Hang He Liang-Bi Chen Lei Li Man-Zhong Liang Xiang-Feng Wang Xi-Gang Liu Guang-Ming He Run-Sheng Chen Li-Geng Ma Xing Wang Deng | 2008 | Molecular Plant2008，1，5： | 25 |
| 9 | 电针和经皮穴位电刺激技术在生殖医学中的应用:专家共识(英文)显示文摘本文从传统针刺治疗不孕症的选穴依据与现代电针/经皮穴位电刺激技术的相关原理着手,从最优刺激参数选择、穴位辨证、疗程与治疗次数的确定及基本原理等方面阐述了电针/经皮穴位电刺激技术在多囊卵巢综合症、取卵镇痛、卵巢储备功能降低、胚胎移植以及男性少/弱精症等生殖领域中的具体应用。首次对电针/经皮穴位电刺激技术在生殖领域优势病种中的应用进行了系统的分析和总结,在最优刺激参数选择、穴位辨证、疗程与治疗次数的确定等方面提出了一系列专家共识,并为临床实践提供了指导意见和理论依据。 | Fan QU Rong LI Wei SUN Ge LIN Rong ZHANG Jing YANG Li TIAN Guo-gang XING Hui JIANG Fei GONG Xiao-yan LIANG Yan MENG Jia-yin LIU Li-ying ZHOU Shu-yu WANG Yan WU Yi-jing HE Jia-yu YE Song-ping HAN Ji-sheng HAN | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 24 |
| 10 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 11 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 12 | Active corrosion protection of super-hydrophobic corrosion inhibitor intercalated Mg-Al layered double hydroxide coating on AZ31 magnesium alloy显示文摘Magnesium alloys,the advanced lightweight structural materials,have been successfully applied in the manufacturing field.Unfortunately,their poor corrosion resistance restrains the potential wide applications.In this work,anti-corrosion coatings were fabricated via the insitu growth of the corrosion inhibitors intercalated magnesium-aluminum layered double hydroxide(Mg-Al LDH)on AZ31 magnesium alloy and then post-sealing it by a super-hydrophobic coating.SEM,XRD,EDS,FTIR,XPS and contact angle test were conducted to analyze physical/chemical features of these coatings.Potentiodynamic polarization curves and electrochemical impedance spectroscopy were recorded to assess the anti-corrosion performance of prepared coatings.Surprisingly,Mg-Al LDH with molybdate intercalation and lauric acid modification achieves the excellent corrosion inhibition performance(99.99%)due to the multicomponent synergistic effect such as the physical protection of Mg-Al LDH,the corrosion inhibition of molybdate and super-hydrophobic properties of lauric acid.This work presents a scientific perspective and novel design philosophy to fabricate the efficient anti-corrosion coating to protect magnesium alloys and then expand their potential applications in other field. | Xin Wang Chuan Jing Yuxiang Chen Xiushuang Wang Gang Zhao Xing Zhang Liang Wu Xiaoying Liu Biqin Dong Yuxin Zhang | 2020 | Journal of Magnesium and Alloys2020，8，1： | 20 |
| 13 | Leap Motion-based virtual reality training for improving motor functional recovery of upper limbs and neural reorganization in subacute stroke patients显示文摘Virtual reality is nowadays used to facilitate motor recovery in stroke patients. Most virtual reality studies have involved chronic stroke patients; however, brain plasticity remains good in acute and subacute patients. Most virtual reality systems are only applicable to the proximal upper limbs(arms) because of the limitations of their capture systems. Nevertheless, the functional recovery of an affected hand is most difficult in the case of hemiparesis rehabilitation after a stroke. The recently developed Leap Motion controller can track the fine movements of both hands and fingers. Therefore, the present study explored the effects of a Leap Motion-based virtual reality system on subacute stroke. Twenty-six subacute stroke patients were assigned to an experimental group that received virtual reality training along with conventional occupational rehabilitation, and a control group that only received conventional rehabilitation. The Wolf motor function test(WMFT) was used to assess the motor function of the affected upper limb; functional magnetic resonance imaging was used to measure the cortical activation. After four weeks of treatment, the motor functions of the affected upper limbs were significantly improved in all the patients, with the improvement in the experimental group being significantly better than in the control group. The action performance time in the WMFT significantly decreased in the experimental group. Furthermore, the activation intensity and the laterality index of the contralateral primary sensorimotor cortex increased in both the experimental and control groups. These results confirmed that Leap Motion-based virtual reality training was a promising and feasible supplementary rehabilitation intervention, could facilitate the recovery of motor functions in subacute stroke patients. The study has been registered in the Chinese Clinical Trial Registry(registration number: Chi CTR-OCH-12002238). | Zun-rong Wang Ping Wang Liang Xing Li-ping Mei Jun Zhao Tong Zhang | 2017 | Neural Regeneration Research2017，12，11： | 19 |
| 14 | Pretreatment HIV drug resistance in adults initiating antiretroviral therapy in China,2017显示文摘Background:After the scale-up of antiretroviral therapy(ART)for HIV infected people,increasing numbers of patients have pretreatment drug resistance(PDR).In this study,the prevalence of PDR was evaluated in adults initiating antiretroviral therapy in China.Methods:Blood samples were obtained from 1943 patients who initiated antiretroviral therapy(ART)in 2017 from 13 provinces or cities in China.Pol sequences were used to analyze drug resistance and construct transmission networks.Logistic regression model was used to estimate the potential factors associated with PDR.Results:In total,1711 eligible patients(76.0%male;87.8%aged≥25 years)were included,of which 117(6.8%)had PDR.The highest rates of PDR were 12.2%in Liangshan Prefecture of Sichuan and 9.3 and 8.9%in Dehong and Lincang Prefecture of Yunnan.A multivariate logistic regression analysis revealed that PDR was significantly higher among intravenous drug users(adjusted Odds Ratio(aOR)=2.64,95%CI:1.57–4.44)and individuals from Liangshan,Dehong,and Lincang(aOR=2.04,95%CI:1.26–3.30).In total,754 sequences were used to generate 164 transmission networks.Five transmission networks had two or three sequences containing the same mutations,two networks contained subjects from Liangshan,and one network contained subjects from Dehong.Conclusions:Overall,the PDR prevalence was moderate,with a particularly high prevalence in areas with severe HIV epidemics.These results indicate the importance of continuous PDR monitoring in patients initiating antiretroviral therapy. | Rui-Hua Kang Shu-Jia Liang Yan-Ling Ma Shu Liang Lin Xiao Xin-Hui Zhang Hong-Yan Lu Xiao-Qin Xu Shui-Bin Luo Xiao-Guang Sun Lin Chen Jian-Mei He Guo-Hui Wu Ling-Jie Liao Hui Xing Yi-Ming Shao Yu-Hua Ruan | 2020 | Infectious Diseases of Poverty2020，9，3： | 17 |
| 15 | Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway显示文摘AIM To explore the induction effects and mechanism of Solanum lyratum Thumb(ST) on human hepatocellularcarcinoma SMMC-7721 cells through the mitochondrial pathway.METHODS The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine m RNA expression of Fas, Fas L, caspase-8, caspase-3, p53 and Bcl-2 genes.RESULTS Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased(P<0.05). Besides, the m RNA expression of Fas L and Bcl-2 significantly decreased(P<0.05) while the m RNA expression of Fas, caspase-8, caspase-3 and p53 increased significantly. When compared with the positive control group, the experimental groups with 5 mg/L ST ethanol extracts showed effects similar to the positive control group.CONCLUSION ST ethanol extracts induced the apoptosis of hepatocellular carcinoma SMMC-7721 cells through up-regulated Fas, caspase-8, caspse-3 and p53, and down-regulated Fas L and Bcl-2 in the mitochondrial pathway. | Xiao-Qiang Mo Hong-Yu Wei Gan-Rong Huang Ling-Yuan Xu Yu-Li Chen Jiang Qi Wei Xian Yan-Chun Qin Lian-Deng Wei Li-Juan Zhao Yan-Qiang Huang Wei Xing Hong-Qin Pu Peng-Ya Wei Chao-Gan Li Qiu-Chun Liang | 2017 | World Journal of Gastroenterology2017，23，6： | 14 |
| 16 | Vascular endothelial growth factor attenuates hepatic sinusoidal capillarization in thioacetamide-induced cirrhotic rats显示文摘AIM: To investigate the effect of vascular endothelial growth factor (VEGF) transfection on hepatic sinusoidal capillarization. METHODS: Enhanced green fluorescent protein (EGFP)/ VEGF transfection was confirmed by immunofluorescence microscopy and immunohistoche-mistry both in primary hepatocytes and in normal liver. Cirrhotic rats were generated by thioacetamide (TAA) administration and then divided into a treatment group, which received injections of 400 μg of plasmid DNA encoding an EGFP- VEGF fusion protein, and a blank group, which received an equal amount of normal saline through the portal vein. The portal vein pressure was measured in the normal and cirrhotic state, in treated and blank groups. The average number of fenestrae per hepatic sinusoid was determined using transmission electron microscopy (TEM), while the relative abundance of VEGF transcripts was examined by Gene array. RESULTS: Green fluorescent protein was observed in the cytoplasms of liver cells under immunofluorescence microscopy 24 h after transfection with EGFP/VEGF plasmid in vitro. Staining with polyclonal antibodies against VEGF illustrated that hepatocytes expressedimmunodetectable VEGF both in vitro and in vitro. There were significant differences in the number of fenestrae and portal vein pressures between normal and cirrhotic rats (7.40 ± 1.71 vs 2.30 ± 1.16 and 9.32 ± 0.85 cmH2O vs 17.92 ± 0.90 cmH2O, P < 0.01), between cirrhotic and treated rats (2.30 ± 1.16 cmH2O vs 4.60 ± 1.65 and 17.92 ± 0.90 cmH2O vs 15.52 ± 0.93 cmH2O, P < 0.05) and between the treatment group and the blank group (4.60 ± 1.65 cmH2O vs 2.10 ± 1.10 cmH2O and 15.52 ± 0.93 cmH2O vs 17.26 ± 1.80 cmH2O, P < 0.05). Gene- array analysis revealed that the relative abundance of transcripts of VEGF family members decreased in the cirrhotic state and increased after transfection. CONCLUSION: Injection of a plasmid encoding VEGF through the portal vein is an effective method to induce the formation of fenestrae and decrease portal vein pressure in cirrhotic rats. Therefore, it may be a good choice for treating hepatic cirrhosis and portal hypertension. | Hao Xu Bao-Min Shi Xiao-Fei Lu Feng Liang Xing Jin Tai-Huang Wu Jian Xu | 2008 | World Journal of Gastroenterology2008，14，15： | 14 |
| 17 | Mechanism of combined use of vitamin D and puerarin in anti-hepatic fibrosis by regulating the Wnt/β-catenin signalling pathway显示文摘AIM To reveal the protective mechanism of the combined use of vitamin D and puerarin in the progression of hepatic fibrosis induced by carbon tetrachloride(CCl4).METHODS Eight-week-old male Wistar rats were randomly divided into a normal control group(C group), a CCl4 group(CCl4 group), a vitamin D group(V group), a puerarin group(P group), and a combined group of vitamin D and puerarin(V + P group), each of which contained ten rats. In this way, we built a rat model of CCl4-induced hepatic fibrosis with intervention by vitamin D, puerarin, or a combination of the two. After eight weeks, the mice were sacrificed to collect serum and liver specimens. Blood was collected to detect the hyaluronic acid(HA). We also measured hydroxyproline(Hyp) and prepared paraffin sections of liver. After Sirius red staining, the liver specimens were observed under a microscope. RT-PCR and western blot analysis were adopted to detect the mRNA and the proteinlevels of Collagen I, Collagen III, Wnt1, and β-catenin in the liver tissues, respectively.RESULTS Hepatic fibrosis was observed in the CCl4 group. In comparison, hepatic fibrosis was attenuated in the V, P, and V + P groups: the HA level in blood and the Hyp level in liver were reduced, and the mRNA levels of Collagen I, Collagen III, Wnt, and β-catenin in liver were also decreased, as well as the protein levels of Wnt1 and β-catenin. Among these groups, the V + P group demonstrated the greatest amelioration of hepatic fibrosis.CONCLUSION The combined application of vitamin D and puerarin is capable of alleviating CCl4-induced hepatic fibrosis of rats. As to the mechanism, it is probably because the combined use is able to silence the Wnt1/β-catenin pathway, suppress the activation of hepatic stellate cells, and reduce the secretion of collagen fibers, therefore improving the anti-hepatic fibrosis effect. | Gan-Rong Huang Si-Jun Wei Yan-Qiang Huang Wei Xing Lu-Yao Wang Ling-Ling Liang | 2018 | World Journal of Gastroenterology2018，24，36： | 12 |
| 18 | Coagulation efficiency and flocs characteristics of recycling sludge during treatment of low temperature and micro-polluted water显示文摘Drinking water treatment sludge,characterized as accumulated suspended solids and organic and inorganic matter,is produced in large quantities during the coagulation process.The proper disposal,regeneration or reuse of sludge is,therefore,a significant environmental issue.Reused sludge at low temperatures is an alternative method to enhance traditional coagulation efficiency.In the present study,the recycling mass of mixed sludge and properties of raw water (such as pH and turbidity) were systematically investigated to optimize coagulation efficiency.We determined that the appropriate dosage of mixed sludge was 60 mL/L,effective initial turbidity ranges were below 45.0 NTU,and optimal pH for DOMs and turbidity removal was 6.5-7.0 and 8.0,respectively.Furthermore,by comparing the flocs characteristics with and without recycling sludge,we found that floc structures with sludge were more irregular with average size growth to 64.7 μm from 48.1 μm.Recycling sludge was a feasible and successful method for enhancing pollutants removal,and the more irregular flocs structure after recycling might be caused by breakage of reused flocs and incorporation of powdered activated carbon into larger flocs structure.Applied during the coagulation process,recycling sludge could be significant for the treatment of low temperature and micro-polluted source water. | Zhiwei Zhou Yanling Yang Xing Li Wei Gao Heng Liang Guibai Li | 2012 | Journal of Environmental Sciences2012，24，6： | 12 |
| 19 | Hyperbaric Oxygen Treatment Improves Hearing Level via Attenuating TLR4/NF-κB Mediated Inflammation in Sudden Sensorineural Hearing Loss Patients显示文摘Objective Hyperbaric oxygen treatment(HBOT)has demonstrated efficacy in improving hearing levels of patients with idiopathic sudden sensorineural hearing loss(ISSHL);however,the underlying mechanisms are not well understood.HBOT alleviates the inflammatory response,which is mediated by Toll-like receptor(TLR)4 and nuclear factor(NF)-κB.In this study we investigated whether HBOT attenuates inflammation in ISHHL patients via alteration of TLR4 and NF-κB expression.Methods ISHHL patients(n=120)and healthy control subjects(n=20)were enrolled in this study.Patients were randomly divided into medicine group treated with medicine only(n=60)and HBO group receiving both HBOT and medicine(n=60).Audiometric testing was performed pre-and posttreatment.TLR4,NF-кB,and TNF-αexpression in peripheral blood of ISSHL patients and healthy control subjects was assessed by ELISA before and after treatment.Results TLR4,NF-κB,and TNF-αlevels were upregulated in ISSHL patients relative to healthy control subjects;the levels were decreased following treatment and were lower in the HBO group than that in the medicine group post-treatment(P<0.05 and P<0.01).Conclusion HBOT alleviates hearing loss in ISSHL patients by suppressing the inflammatory response induced by TLR4 and NF-κB signaling. | LIU Xue Hua LIANG Fang JIA Xing Yuan ZHAO Lin ZHOU Yan YANG Jing | 2020 | Biomedical and Environmental Sciences2020，33，5： | 12 |
| 20 | New strategy for designing orangish-redemitting phosphor via oxygen-vacancyinduced electronic localization显示文摘Phosphor-converted white-light-emitting diodes(pc-WLED)have been extensively employed as solid-state lighting sources,which have a very important role in people’s daily lives.However,due to the scarcity of the red component,it is difficult to realize warm white light efficiently.Hence,red-emitting phosphors are urgently required for improving the illumination quality.In this work,we develop a novel orangish-red La_(4)GeO_(8):Bi^(3+) phosphor,the emission peak of which is located at 600 nm under near-ultraviolet(n-UV)light excitation.The full width at half maximum(fwhm)is 103 nm,the internal quantum efficiency(IQE)exceeds 88%,and the external quantum efficiency(EQE)is 69%.According to Rietveld refinement analysis and density functional theory(DFT)calculations,Bi^(3+) ions randomly occupy all La sites in orthorhombic La_(4)GeO_(8).Importantly,the oxygen-vacancy-induced electronic localization around the Bi3+ions is the main reason for the highly efficient orangish-red luminescence.These results provide a new perspective and insight from the local electron structure for designing inorganic phosphor materials that realize the unique luminescence performance of Bi^(3+) ions. | Yi Wei Gongcheng Xing Kang Liu Guogang Li Peipei Dang Sisi Liang Min Liu Ziyong Cheng Dayong Jin Jun Lin | 2019 | Light(Science & Applications)2019，8，1： | 12 |
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