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| 1 | Evidence chain-based causality identification in herb-induced liver injury: exemplification of a well-known liver-restorative herb Polygonum multiflorum显示文摘草药的药最近在美国作为导致药的肝损害(DILI ) 的第二个很普通的原因被认出了。然而,识别一些植物的 DILI 诱发性的可靠方法例如 Heshouwu (蓼 multiflorum 的弄干的根) ,留下缺乏。在这研究,有肝机能障碍和 Heshouwu DILI 的 147 个报导文学的案例的 12 307 个住院病人的一个总数被屏蔽。一个一般算法仅仅显示了那 22.5%(9/40 ) 并且(45/147 ) 分别地, 30.6% 所有住院和文学案例报告表明 Heshouwu 的 DILI 诱发性的高概率。由对比,(19/20 )95% 生药学,植物化学,和 metabolomic 有希望地调查的所有情况测试展出高度可能的诱发性,包括不正确地以前被归因的一个病人和从由 pharmacognostic 证据的 Heshouwu 诱发性被排除的一个案例。污染也是的毒素(重金属,杀虫剂,和 mycotoxins ) 从 Heshouwu DILI 诱发性排除了。为 Heshouwu DILI 诊断地址安全的这些屏蔽方法的客观性担心考虑包含芪的草药的药和饮食的补充。 | Jiabo Wang Zhijie Ma Ming Niu Yun Zhu Qingsheng Liang Yanling Zhao Jingyuan Song Zhaofang Bai Yaming Zhang Ping Zhang Na Li Yakun Meng Qi Li Lushan Qin Guangju Teng Junling Cao Baosen Li Shilin Chen Yonggang Li Zhengsheng Zou Honghao Zhou Xiaohe Xiao | 2015 | Frontiers of Medicine2015，9，4： | 47 |
| 2 | Facile Synthesis and Application of Ag-Chemically Converted Graphene Nanocomposite显示文摘An in situ chemical synthesis approach has been employed to prepare an Ag-chemically converted graphene (CCG) nanocomposite. The reduction of graphene oxide sheets was accompanied by generation of Ag nanoparticles. The structure and composition of the nanocomposites were confirmed by means of transmission electron microscopy (TEM), atomic force microscopy (AFM) and X-ray diffraction. TEM and AFM results suggest a homogeneous distribution of Ag nanoparticles (5-10 nm in size) on CCG sheets. The intensities of the Raman signals of CCG in such nanocomposites are greatly increased by the attached silver nanoparticles, i.e., there is surface-enhanced Raman scattering activity. In addition, it was found that the antibacterial activity of free Ag nanoparticles is retained in the nanocomposites, which suggests they can be used as graphene-based biomaterials. | Jianfeng Shen Min Shi Na Li Bo Yan Hongwei Ma Yizhe Hu Mingxin Ye | 2010 | Nano Research2010，3，5： | 29 |
| 3 | Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine显示文摘Gegen Qinlian Decoction(GQD),a traditional Chinese medicine(TCM)formula,has long been used for the treatment of common metabolic diseases,including type 2 diabetes mellitus.However,the main limitation of its wider application is ingredient complexity of this formula.Thus,it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action.Here,we compared the effects of GQD and berberine,a hypothetical key active pharmaceutical ingredient of GQD,on a diabetic rat model by comprehensive analyses of gut microbiota,short-chain fatty acids,proinflammatory cytokines,and ileum transcriptomics.Our results show that berberine and GQD had similar effects on lowering blood glucose levels,modulating gut microbiota,inducing ileal gene expression,as well as relieving systemic and local inflammation.As expected,both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria,including Faecalibacterium and Roseburia,thereby attenuating intestinal inflammation and lowering glucose.Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD.Moreover,concentration of serum proinflammatory cytokines and expression of immune-related genes,including Nfkb1,Stat1,and Ifnrg1,in pancreatic islets were significantly reduced after treatment.Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota.The strategy employed would facilitate further standardization and widespread application of TCM in many diseases. | Xizhan Xu Zezheng Gao Fuquan Yang Yingying Yang Liang Chen Lin Han Na Zhao Jiayue Xu Xinmiao Wang Yue Ma Lian Shu Xiaoxi Hu Na Lyu Yuanlong Pan Baoli Zhu Linhua Zhao Xiaolin Tong Jun Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 29 |
| 4 | Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats显示文摘AIM: To observe the efficacy and mechanism of grainsized moxibustion at different acupoints in a rat model of ulcerative colitis(UC). METHODS: Sprague-Dawley rats were randomly divided into control, UC model, grain-sized moxibustion at a single acupoint(CV 12), grain-sized moxibustion at two acupoints(CV 12 and CV 4), grain-sized moxibustion at three acupoints(CV 12, CV 4, and ST 36), and medication groups(n = 8/group). The UC model was established by enema of trinitrobenzene sulfonic acid. Direct moxibustion was used once a day for 7 d. Disease activity index(DAI) was evaluated before and after the treatment. Morphologic changes of intestinal tissue were observed under an optical microscope. The expression of tumor necrosis factor(TNF)-α and p38 mitogen-activated protein kinase(p38MAPK) in colonic tissue was detected using Western blot, and the levels of occludin and zonula occludens-1(ZO-1) m RNAs were detected using reverse transcription PCR. RESULTS: Compared with the control group, the intestinal mucosae were incomplete in the model group, glandular structures were irregular, and submucosae were edematous, hyperemic, and infiltrated with inflammatory cells. The DAI scores and expression ofTNF-α and p38 MAPK were increased significantly in the model group compared to controls(P s < 0.01), while the m RNA levels of occludin and ZO-1 were reduced significantly(P s < 0.01). Compared with the model group, colonic mucosa and the arrangement of glands were complete and regular in the treatment groups. DAI scores and the expression of TNF-α and p38 MAPK were reduced significantly in moxibustion groups compared to controls(P s < 0.01), while the m RNA levels of occludin and ZO-1 were increased significantly(P s < 0.01). The improvements in the above indices in the three acupoints group and the medication group were superior to those in the single and two acupoints groups(all P < 0.05). CONCLUSION: Reduction of TNF-α and p38 MAPK and increased expression of occludin and ZO-1 in colonic tissue represent a potential mechanism for improved intestinal mucosal tissue repair with grain-sized moxibustion. | Tie-Ming Ma Na Xu Xian-De Ma Zeng-Hua Bai Xing Tao Hong-Chi Yan | 2016 | World Journal of Gastroenterology2016，22，8： | 30 |
| 5 | Latest Progress of the Chinese Meteorological Satellite Program and Core Data Processing Technologies显示文摘In this paper,the latest progress,major achievements and future plans of Chinese meteorological satellites and the core data processing techniques are discussed.First,the latest three FengYun(FY)meteorological satellites(FY-2H,FY-3D,and FY-4A)and their primary objectives are introduced Second,the core image navigation techniques and accuracies of the FY meteorological satellites are elaborated,including the latest geostationary(FY-2/4)and polar-orbit(FY-3)satellites.Third,the radiometric calibration techniques and accuracies of reflective solar bands,thermal infrared bands,and passive microwave bands for FY meteorological satellites are discussed.It also illustrates the latest progress of real-time calibration with the onboard calibration system and validation with different methods,including the vicarious China radiance calibration site calibration,pseudo invariant calibration site calibration,deep convective clouds calibration,and lunar calibration.Fourth,recent progress of meteorological satellite data assimilation applications and quantitative science produce are summarized at length.The main progress is in meteorological satellite data assimilation by using microwave and hyper-spectral infrared sensors in global and regional numerical weather prediction models.Lastly,the latest progress in radiative transfer,absorption and scattering calculations for satellite remote sensing is summarized,and some important research using a new radiative transfer model are illustrated. | Peng ZHANG Qifeng LU Xiuqing HU Songyan GU Lei YANG Min MIN Lin CHEN Na XU Ling Sun Wenguang BAI Gang MA Di XIAN | 2019 | Advances in Atmospheric Sciences2019，36，9： | 26 |
| 6 | Compensation trends of the angulation of first molars:retrospective study of 1403 malocclusion cases显示文摘We investigated the compensatory trends of mesiodistal angulation of first molars in malocclusion cases. We compared differences in the angulation of first molars in different developmental stages, malocclusion classifications and skeletal patterns. The medical records and lateral cephalogrammes of 1 403 malocclusion cases taken before treatment were measured to evaluate compensation of molar angulation in relation to the skeletal jaw. The cases were stratified by age, Angle classification and skeletal patterns. Differences in the mesiodistal angulation of the first molars were compared among the stratifications. We observed three main phenomena. First,angulation of the upper first molar varied significantly with age and tipped most distally in cases aged,12 years and least distally in cases aged.16 years. The lower first molar did not show such differences. Second, in Angle Class II or skeletal Class II cases, the upper first molar was the most distally tipped, the lower first molar was the most mesially tipped, and opposite angulation compensation was observed in Class III cases. Third, in high-angle cases, the upper and lower first molars were the most distally tipped, and opposite angulation compensation was observed in low-angle cases. These data suggest that the angulation of the molars compensated for various growth patterns and malocclusion types. Hence, awareness of molar angulation compensation would help to adjust occlusal relationships, control anchorage and increase the chances of long-term stability. | Hong Su Bing Han Sa Li Bin Na Wen Ma Tian-Min Xu | 2014 | International Journal of Oral Science2014，6，3： | 22 |
| 7 | Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment. | Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 23 |
| 8 | Crystal structure and biochemical analyses reveal Beclin 1 as a novel membrane binding protein显示文摘Beclin 1 基因是 haplo 不够的肿瘤 suppressor 并且在 autophagy 起一个必要作用。然而, Beclin 1 由工作的分子的机制仍然保持大部分未知。这里,我们在 1.6 Å 报导 Beclin 1 的 evolutionarily 保存的领域(ECD ) 的水晶结构;分辨率。Beclin 1 ECD 展览一以前 unreported 褶层,与在中央轴附近相称性地安排的三次结构的重复。Beclin 1 ECD 定义膜绑定领域的一个新奇的类,与为与 cardiolipin 充实的类脂化合物膜的强壮的偏爱。在 Beclin 1 ECD 的一个表面环的尖端,包括三芳香的氨基酸,与类脂化合物膜充当一根恐水病的手指到伙伴,因而导致膜和 liposomes 的变丑。这些芳香的残余的变化使 Beclin 1 变为不能稳定地在 vitro 与类脂化合物膜联系并且不能充分在 vivo 在 Beclin 1-knockdown 房间救 autophagy。这些观察为译解形成一个重要框架 Beclin 1 的生物功能。 | Weljlao Huang Wooyoung Chol Wanqiu Hu Na Mi Qiang Guo Meisheng Ma Mei Liu Yuan Tian Peilong Lu Feng-Liang Wang Haiteng Deng Lei Liu Ning Gao Li Yu Yigong Shi | 2012 | Cell Research2012，22，3： | 21 |
| 9 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 10 | Pretreatment with a combination of ligustrazine and berberine improves cardiac function in rats with coronary microembolization显示文摘瞄准:我们证明了 ligustrazine 和 berberine 的联合在老鼠在血小板激活和煽动性的反应上生产更有效的抑制尖锐心肌的梗塞与也相比代理人独自一个。在我们评估了的这研究,在一只老鼠的 ligustrazine 和 berberine 的联合的有益的效果冠的 microembolization (CME ) 为 .Methods 建模:SD 老鼠与 ligustrazine, berberine, ligustrazine+berberine,或 clopidogrel 被对待 2 个星期。当处理完成了时, CME 被钠 laurate 的注射导致进左室,当妨碍上升主动脉时。所有老鼠为血液动力学的大小被把管子插进。血样品为生物化学的分析,流动 cytometry,和 ELISA 被收集。心纸巾为组织病理学说和随后的蛋白质 analyses.Results 被孤立:有 ligustrazine 的联合的预告的处理(27 mg· kg −1·d−1) 和 berberine (90 mg· kg −1·d−1) 显著地改善了心脏的功能,和减少的心肌的坏死,煽动性的房间渗入, microthrombosis 和浆液 CK-MB 在 CME 铺平老鼠。另外,这联合显著地减少了血浆 ET-1 层次和 von Willebrand 因素,禁止的导致自动数据处理的血小板激活,和减少的 TNFα, IL-1β,在浆液的 ICAM-1 和 RANTES 层次和心纸巾。这联合的保护的效果是比 ligustrazine 或 berberine 的那些更突出的到那些积极控制 clopidogrel 独自一个,却可比较(6.75 mg· kg −1·d−1).Conclusion:ligustrazine 和 berberine 的联合显著地经由包含 antiplatelet 和反煽动性的效果的机制在老鼠 CME 模型改进了心脏的功能。 | Ying ZHANG Xiao-juan MA Chun-yu GUO Ming-ming WANG Na KOU Hui-min MAO Da-zhuo SHI | 2016 | Acta Pharmacologica Sinica2016，37，4： | 19 |
| 11 | Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an aggressive malignancy with extremely skewed ethnic and geographic distributions.Increasing evidence indicates that targeting the tumor microenvironment(TME)represents a promising therapeutic approach in NPC,highlighting an urgent need to deepen the understanding of the complex NPC TME.Here,we generated single-cell transcriptome profiles for 7581 malignant cells and 40,285 immune cells from fifteen primary NPC tumors and one normal sample.We revealed malignant signatures capturing intratumoral transcriptional heterogeneity and predicting aggressiveness of malignant cells.Diverse immune cell subtypes were identified,including novel subtypes such as CLEC9A^+dendritic cells(DCs).We further revealed transcriptional regulators underlying immune cell diversity,and cell–cell interaction analyses highlighted promising immunotherapeutic targets in NPC.Moreover,we established the immune subtype-specific signatures,and demonstrated that the signatures of macrophages,plasmacytoid dendritic cells(pDCs),CLEC9A^+DCs,natural killer(NK)cells,and plasma cells were significantly associated with improved survival outcomes in NPC.Taken together,our findings represent a unique resource providing in-depth insights into the cellular heterogeneity of NPC TME and highlight potential biomarkers for anticancer treatment and risk stratification,laying a new foundation for precision therapies in NPC. | Yu-Pei Chen Jian-Hua Yin Wen-Fei Li Han-Jie Li Dong-Ping Chen Cui-Juan Zhang Jia-Wei Lv Ya-Qin Wang Xiao-Min Li Jun-Yan Li Pan-Pan Zhang Ying-Qin Li Qing-Mei He Xiao-Jing Yang Yuan Lei Ling-Long Tang Guan-Qun Zhou Yan-Ping Mao Chen Wei Ke-Xu Xiong Hong-Bo Zhang Shi-Da Zhu Yong Hou Ying Sun Michael Dean Ido Amit Kui Wu Dong-Ming Kuang Gui-Bo Li Na Liu Jun Ma | 2020 | Cell Research2020，30，11： | 19 |
| 12 | Characteristics of sewage sludge and distribution of heavy metal in plants with amendment of sewage sludge显示文摘In order to better understand land application of sewage sludge, the characterization of heavy metals and organic pollutants were investigated in three different sewage sludges in Shanghai City, China. It was found that the total concentrations of Cd in all of sewage sludge and total concentrations of Zn in Jinshan sewage sludge, as well as those of Zn, Cu, and Ni in Taopu sludge are higher than Chinese regulation limit of pollutants for sludge to be used in agriculture. Leachability of Hg in all of studied samples and that of Cd in Taopu sewage sludge exceed the limit values of waste solid extraction standard in China legislation. Based on the characteristics for three kinds of sewage sludge, a pot experiment was conducted to investigate the effect of soil amended with Quyang sewage sludge on the accumulation of heavy metal by Begonia semperflorens-hybr; Ophiopogon japonicus (L.F.) Ker-Gaw; Loropetalum chindense-var. rubrum; Dendranthema morifolium; Viola tricolor; Antirrhinum majus; Buxus radicans Sieb; Viburnum macrocephalum; Osmanthus fragrans Lour; Cinnamomum camphora siebold and Ligustrum lucidum ait. Results showed that 8 species of plant survived in the amended soil, and moreover they flourished as well as those cultivated in the control soil. The heavy metal concentration in plants varied with species, As, Pb, Cd and Cr concentration being the highest in the four herbaceous species studied, particularly in the roots of D. morifolium. These plants, however, did not show accumulator of As, Pb, Cd and Cr. The highest concentration of Ni and Hg was found in the roots of D. morifolium, followed by the leaves of B. semperflorens-hybr. Levels of Zn and Cu were much higher in D. morifolium than in the other plant species. D. morifolium accumulated Ni, Hg, Cu and Zn, which may contribute to the decrease of heavy metal contents in the amended soil. Treatment with sewage sludge did not significantly affect the uptake of heavy metals by the L. chindense-var. rubrum, however, it significantly affected the uptake of heavy metals by D. morifolium. | DAI Jia-yin CHEN Ling ZHAO Jian-fu MA Na | 2006 | Journal of Environmental Sciences2006，18，6： | 18 |
| 13 | Ionic Liquid-Assisted One-Step Hydrothermal Synthesis of TiO2-Reduced Graphene Oxide Composites显示文摘我们为可溶的减少的 graphene 氧化物表(RGO ) 和钛氧化物(TiO2 ) 的准备的制造表明了灵巧、有效的策略用一出修改一步舞的 nanoparticle-RGO composites 热水的方法。graphene 氧化物能容易作为 reductant 与维生素酸在 solvothermal 条件下面被减少,这被发现,与 RGO 表面上的 TiO2 粒子的伴随物生长。TiO2-RGO 合成被 Fourier 变换彻底地描绘了红外线的光谱学,拉曼光谱学, X 光检查衍射, X 光检查光电子光谱学,和 thermogravimetric 分析。显微镜学技术(扫描电子显微镜学,原子力量显微镜学,和传播电子显微镜学) 被采用了探查词法特征以及调查 RGO 表的脱落。TiO2-RGO 氢的合成展出优秀光催化进化。 | Jianfeng Shen Min Shi Bo Yan Hongwei Ma Na Li Mingxin Ye | 2011 | Nano Research2011，4，8： | 18 |
| 14 | Expression of p57^(kip2), Rb protein and PCNA and their relationships with clinicopathology in human pancreatic cancer显示文摘AIM: To investigate the effects of inhibiting factor of cellcycle regulation p57kip2, retinoblastinoma protein (Rb protein)and proliferating cell nuclear antigen (PCNA) in the genesisand progression of human pancreatic cancer.METHODS: The expression of p57kip2, Rb protein and PCNAin tumor tissues and adjacent tissues of 32 patients withpancreatic cancer was detected with SP immunohistochemicaltechnique.RESULTS: p57kip2 protein positive-expression rate in tumortissues of pancreatic cancer was 46.9 %, which was lowerthan that in adjacent pancreatic tissues (75.0 %) (x2=5.317,P<0.05), p57kip2 protein positive-expression correlatedsignificantly with tumor cell differentiation (well-differentiationversus moderate or low-differentiation, P<0.05) but did notcorrelate significantly with lymph node metastasis (lymph nodemetastasis versus non-lymph node metastasis, P>0.05); Rbgene protein positive-expression rate in tumor tissues was50.0 %, which was also lower than that in adjacent pancreatictissues (78.1%) (x2=5.497, P<0.05); PCNA positive-expression rate was 71.9 %, being higher than that inadjacent pancreatic tissues (43.8 %) (x2=5.189, P<0.05),PCNA positive-expression also correlated significantly withtumor cell differentiation and lymph node metastasis (well-differentiation versus moderate or low- differentiation, lymphnode metastasis versus non-lymph node metastasis, P<0.05).Rb protein positive-expression rate in the tumor tissues ofp57kip2 protein positive-expression group was 53.3 %; andRb protein positive-expression rate in the tumor tissues ofp57kip2 protein negative-expression group was 47.1%. Therewas no significant relationship between the two groups(r=0.16507, P>0.05).CONCLUSION: The decreased expression of p57kip2, Rbprotein or over-expression of PCNA protein might contributeto the genesis or progression of pancreatic cancer, p57kip2,Rb protein and PCNA may play an important role in genesisand progression of pancreatic cancer. | Hui Yue Yan-Li Na Xin-Li Feng Shu-Ren Ma Fu-Lin Song Bo Yang Department of Gastroenterology and Pathology, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China | 2003 | World Journal of Gastroenterology2003，9，2： | 15 |
| 15 | Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger显示文摘Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain(FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity(ORAC), cellular antioxidant activity(CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species(ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-?, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide(LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid. | YIN Zhi Na WU Wen Jia SUN Chong Zhen LIU Hui Fan CHEN Wen Bo ZHAN Qi Ping LEI Zhuo Gui XIN Xuan MA Juan Juan YAO Kun MIN Tian ZHANG Meng Meng WU Hui | 2019 | Biomedical and Environmental Sciences2019，32，1： | 14 |
| 16 | The Qitai radio telescope显示文摘This study presents a general outline of the Qitai radio telescope(QTT)project.Qitai,the site of the telescope,is a county of Xinjiang Uygur Autonomous Region of China,located in the east Tianshan Mountains at an elevation of about 1800 m.The QTT is a fully steerable,Gregorian-type telescope with a standard parabolic main reflector of 110 m diameter.The QTT has adopted an umbrella support,homology-symmetric lightweight design.The main reflector is active so that the deformation caused by gravity can be corrected.The structural design aims to ultimately allow high-sensitivity observations from 150 MHz up to115 GHz.To satisfy the requirements for early scientific goals,the QTTwill be equipped with ultra-wideband receivers and large field-of-view multi-beam receivers.A multi-function signal-processing system based on RFSo C and GPU processor chips will be developed.These will enable the QTT to operate in pulsar,spectral line,continuum and Very Long Baseline Interferometer(VLBI)observing modes.Electromagnetic compatibility(EMC)and radio frequency interference(RFI)control techniques are adopted throughout the system design.The QTT will form a world-class observational platform for the detection of lowfrequency(nano Hertz)gravitational waves through pulsar timing array(PTA)techniques,pulsar surveys,the discovery of binary black-hole systems,and exploring dark matter and the origin of life in the universe.The QTT will also play an important role in improving the Chinese and international VLBI networks,allowing high-sensitivity and high-resolution observations of the nuclei of distant galaxies and gravitational lensing systems.Deep astrometric observations will also contribute to improving the accuracy of the celestial reference frame.Potentially,the QTT will be able to support future space activities such as planetary exploration in the solar system and to contribute to the search for extraterrestrial intelligence. | Na Wang Qian Xu Jun Ma Zhiyong Liu Qi Liu Hailong Zhang Xin Pei Maozheng Chen Richard N.Manchester Kejia Lee Xingwu Zheng Hans J.Kärcher Wulin Zhao Hongwei Li Dongwei Li Martin Süss Matthias Reichert Zhongyi Zhu Congsi Wang Mingshuai Li Rui Li Ning Li Guljaina Kazezkhan Wenming Yan Gang Wu Lang Cui Ming Zhang Haitao Li | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，8： | 14 |
| 17 | CXCR7/CXCL12 axis is involved in lymph node and liver metastasis of gastric carcinoma显示文摘AIM To investigate the role of CXC chemokine receptor(CXCR)-7 and CXCL12 in lymph node and liver metastasis of gastric carcinoma.METHODS In 160 cases of gastric cancer, the expression of CXCR7 and CXCL12 in tumor and matched tumoradjacent non-cancer tissues, in the lymph nodes around the stomach and in the liver was detected using immunohistochemistry to analyze the relationship between CXCR7/CXCL12 expression and clinicopathological features and to determine whether CXCR7 and CXCL12 constitute a biological axis to promote lymph node and liver metastasis of gastric cancer. Furthermore, the CXCR7 gene was silenced and overexpressed in human gastric cancer SGC-7901 cells, and cell proliferation, migration and invasiveness were measured by the MTT, wound healing and Transwell assays, respectively. RESULTS CXCR7 expression was up-regulated in gastric cancer tissues(P = 0.011). CXCR7/CXCL12 expression was significantly related to poor tumor differentiation, high tumor stage and lymph node(r = 0.338, P = 0.000) and liver metastasis(r = 0.629, P = 0.000). The expression of CXCL12 in lymph node and liver metastasis was higher than that in primary gastric cancer tissues(χ2 = 6.669, P = 0.010; χ2 = 25379, P = 0.000), and the expression of CXCL12 in lymph node and liver metastasis of gastric cancer was consistent with the positive expression of CXCR7 in primary gastric cancer(r = 0.338, P = 0.000; r = 0.629, P = 0.000). Overexpression of the CXCR7 gene promoted cell proliferation, migration and invasion. Silencing of the CXCR7 gene suppressed SGC-7901 cell proliferation, migration and invasion. Human gastric cancer cell lines expressed CXCR7 and showed vigorous proliferation and migratory responses to CXCL12.CONCLUSION The CXCR7/CXCL12 axis is involved in lymph node and liver metastasis of gastric cancer. CXCR7 is considered a potential therapeutic target for the treatment of gastric cancer. | Qi Xin Na Zhang Hai-Bo Yu Qin Zhang Yan-Fen Cui Chuan-Shan Zhang Zhe Ma Yan Yang Wei Liu | 2017 | World Journal of Gastroenterology2017，23，17： | 14 |
| 18 | 6min步行试验中不同指令对行走距离的影响显示文摘6min步行试验(6-minwalktest,6MWT)作为测定心肺功能的一种方法,一直以使患者走的尽可能远为目标,然而,特定的步行指令对患者6MWT测定的影响尚不清楚。方法:纳入肺动脉高压(pulmonaryarterialhypertension,PAH)、特发性肺间质纤维化(idiopathicpulmonaryfibrosis,IPF)和其他类型的间质性肺疾病(interstitiallungdisease,ILD)患者, | 刘莉 叶鹏 Weir NA Brown AW Shlobin OA Smith MA Reffett T Battle E Ahmad S Nathan SD | 2014 | 中华高血压杂志2014，22，2： | 13 |
| 19 | The Performance of Whole Genome Amplification Methods and Next-Generation Sequencing for Pre-Implantation Genetic Diagnosis of Chromosomal Abnormalities显示文摘Reliable and accurate pre-implantation genetic diagnosis(PGD) of patient's embryos by next-generation sequencing(NGS) is dependent on efficient whole genome amplification(WGA) of a representative biopsy sample. However, the performance of the current state of the art WGA methods has not been evaluated for sequencing. Using low template DNA(15 pg) and single cells, we showed that the two PCR-based WGA systems Sure Plex and MALBAC are superior to the REPLI-g WGA multiple displacement amplification(MDA) system in terms of consistent and reproducible genome coverage and sequence bias across the 24 chromosomes, allowing better normalization of test to reference sequencing data. When copy number variation sequencing(CNV-Seq) was applied to single cell WGA products derived by either Sure Plex or MALBAC amplification, we showed that known disease CNVs in the range of 3e15 Mb could be reliably and accurately detected at the correct genomic positions. These findings indicate that our CNV-Seq pipeline incorporating either Sure Plex or MALBAC as the key initial WGA step is a powerful methodology for clinical PGD to identify euploid embryos in a patient's cohort for uterine transplantation. | Na Li Li Wang Hui Wang Minyue Ma Xiaohong Wang Yi Li Wenke Zhang Jianguang Zhang David S.Cram Yuanqing Yao | 2015 | Journal of Genetics and Genomics2015，42，4： | 13 |
| 20 | Peroxisome proliferator-activated receptor-γ is essential in the pathogenesis of gastric carcinoma显示文摘AIM:To investigate whether peroxisome proliferator-activated receptor γ (PPAR-γ) is expressed in human gastric carcinoma and whether PPAR-γ is a potential target for gastric carcinoma therapy.METHODS: PPAR-γ protein in gastric carcinoma was examined by immunohistochemistry. In the gastric carcinoma cell line MGC803, PPAR-γ, survivin, Skp2 and p27 protein and mRNA were examined by Western blotting and real-time reverse transcription-polymerase chain reaction, respectively; proliferation was examined by MTT; apoptosis was examined by chromatin staining with Hoechst 33342 and fluorescence activated cell sorting (FACS). and cell cycle was examined by FACS; the knockdown of PPAR-γ was done by RNA interference.RESULTS: A high level of expression of PPAR-γ was observed in human gastric carcinoma and in a human gastric carcinoma cell line MGC803. The PPAR-γ agonist 15-deoxy-12,14-prostaglandin J2 (15d-PGJ2) inhibited growth, and induced apoptosis and G1/G0 cell cycle arrest in MGC803 cells in a concentration-dependent and time-dependent manner. The effect of 15d-PGJ2 on MGC803 cells was not reversed by the selective and irreversible antagonist GW9662 for PPAR-γ. Furthermore, survivin and Skp2 expression were decreased, whereasp27 expression was enhanced following 15d-PGJ2 treatment in a dose-dependent manner in MGC803 cells. Interestingly, we also found that small interfering RNA for PPAR-γ inhibited growth and induced apoptosis in MGC803 cells. The inhibition of PPAR-γ function may be a potentially important and novel modality for treatment and prevention of gastric carcinoma.CONCLUSION: A PPAR-γ agonist inhibited growth of human gastric carcinoma MGC803 cells by inducing apoptosis and G1/G0 cell cycle arrest with the involvement of survivin, Skp2 and p27 and not via PPAR-γ. | Xiu-Mei Ma Hong Yu Na Huai | 2009 | World Journal of Gastroenterology2009，15，31： | 13 |
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