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| 1 | Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression显示文摘Recently,increasing evidence has suggested the association between gut dysbiosis and Alzheimer's disease(AD)progression,yet the role of gut microbiota in AD pathogenesis remains obscure.Herein,we provide a potential mechanistic link between gut microbiota dysbiosis and neuroinflammation in AD progression.Using AD mouse models,we discovered that,during AD progression,the alteration of gut microbiota composition leads to the peripheral accumulation of phenylalanine and isoleucine,which stimulates the differentiation and proliferation of pro-inflammatory T helper 1(Thl)cells.The brain-infiltrated peripheral Th1 immune cells are associated with the Ml microglia aaivation,contributing to AD-associated neuroinflammation.Importantly,the elevation of phenylalanine and isoleucine concentrations and the increase of Th1 cell frequency in the blood were also observed in two small independent cohorts of patients with mild cognitive impairment(MCI)due to AD.Furthermore,GV-971,a sodium oligomannate that has demonstrated solid and consistent cognition improvement in a phase 3 clinical trial in China,suppresses gut dysbiosis and the associated phenylalanine/isoleucine accumulation,harnesses neuroinflammation and reverses the cognition impairment.Together,our findings highlight the role of gut dysbiosis-promoted neuroinflammation in AD progression and suggest a novel strategy for AD therapy by remodelling the gut microbiota. | Xinyi Wang Guangqiang Sun Teng Feng Jing Zhang Xun Huang Tao Wang Zuoquan Xie Xingkun Chu Jun Yang Huan Wang Shuaishuai Chang Yanxue Gong Lingfei Ruan Guanqun Zhang Siyuan Yan Wen Lian Chen Du Dabing Yang Qingli Zhang Feifei Lin Jia Liu Haiyan Zhang Changrong Ge Shifu Xiao Jian Ding Meiyu Geng | 2019 | Cell Research2019，29，10： | 192 |
| 2 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 3 | Deep Learning for Wireless Physical Layer: Opportunities and Challenges显示文摘Machine learning(ML) has been widely applied to the upper layers of wireless communication systems for various purposes, such as deployment of cognitive radio and communication network. However, its application to the physical layer is hampered by sophisticated channel environments and limited learning ability of conventional ML algorithms. Deep learning(DL) has been recently applied for many fields, such as computer vision and natural language processing, given its expressive capacity and convenient optimization capability. The potential application of DL to the physical layer has also been increasingly recognized because of the new features for future communications, such as complex scenarios with unknown channel models, high speed and accurate processing requirements; these features challenge conventional communication theories. This paper presents a comprehensive overview of the emerging studies on DL-based physical layer processing, including leveraging DL to redesign a module of the conventional communication system(for modulation recognition, channel decoding, and detection) and replace the communication system with a radically new architecture based on an autoencoder. These DL-based methods show promising performance improvements but have certain limitations, such as lack of solid analytical tools and use of architectures that are specifically designed for communication and implementation research, thereby motivating future research in this field. | Tianqi Wang Chao-Kai Wen Hanqing Wang Feifei Gao Tao Jiang Shi Jin | 2017 | China Communications2017，14，11： | 51 |
| 4 | Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility显示文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF. | Lijun Ding Guijun Yan Bin Wang Lu Xu Yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang Yanjun Yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou Yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun | 2018 | Science China(Life Sciences)2018，61，12： | 43 |
| 5 | Progress and recent trend in MILD combustion显示文摘Moderate or intense low oxygen dilution(MILD)combustion plays a significant role in the mitigation of combustion-generated pollutants and greenhouse gases whilst meeting thermal efficiency needs.However,due to the lack of the fundamental knowledge on this combustion,there is a misconception that MILD combustion should be established by high preheating of the air,which has limited its application.Our research and development on this combustion has been performed for several years. We have found that the requirements for establishing the MILD combustion are more relaxed than previously.It is also revealed that this combustion of different type,i.e.,non-premixed,partially premixed and fully premixed,can be achieved by firing various fuels(i.e.,gaseous,liquid and solid fuels).It is suggested that the application of the MILD combustion can be expanded significantly.The present review summarizes the progress and recent trend made in the R&D of this combustion and recommends further fundamental studies for improving our knowledge and widening its applications. | LI PengFei MI JianChun DALLY B B WANG FeiFei WANG Lin LIU ZhaoHui CHEN Sheng ZHENG ChuGuang | 2011 | Science China(Technological Sciences)2011，54，2： | 31 |
| 6 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 7 | Mechanisms of resistance to third-generation EGFR tyrosine kinase inhibitors显示文摘 | Shuhang Wang Yongping Song Feifei Yan Delong Liu | 2016 | Frontiers of Medicine2016，10，4： | 21 |
| 8 | Characterization of drug responses of mini patient-derived xenografts in mice for predicting cancer patient clinical therapeutic response显示文摘Background:Patient-derived organoids and xenografts(PDXs)have emerged as powerful models in functional diag-nostics with high predictive power for anticancer drug response.However,limitations such as engraftment failure and time-consuming for establishing and expanding PDX models followed by testing drug efficacy,and inability to subject to systemic drug administration for ex vivo organoid culture hinder realistic and fast decision-making in selecting the right therapeutics in the clinic.The present study aimed to develop an advanced PDX model,namely MiniPDX,for rapidly testing drug efficacy to strengthen its value in personalized cancer treatment.Methods:We developed a rapid in vivo drug sensitivity assay,OncoVee®MiniPDX,for screening clinically relevant regimens for cancer.In this model,patient-derived tumor cells were arrayed within hollow fiber capsules,implanted subcutaneously into mice and cultured for 7 days.The cellular activity morphology and pharmacokinetics were systematically evaluated.MiniPDX performance(sensitivity,specificity,positive and negative predictive values)was examined using PDX as the reference.Drug responses were examined by tumor cell growth inhibition rate and tumor growth inhibition rate in PDX models and MiniPDX assays respectively.The results from MiniPDX were also used to evaluate its predictive power for clinical outcomes.Results:Morphological and histopathological features of tumor cells within the MiniPDX capsules matched those both in PDX models and in original tumors.Drug responses in the PDX tumor graft assays correlated well with those in the corresponding MiniPDX assays using 26 PDX models generated from patients,including 14 gastric cancer,10 lung cancer and 2 pancreatic cancer.The positive predictive value of MiniPDX was 92%,and the negative predictive value was 81%with a sensitivity of 80%and a specificity of 93%.Through expanding to clinical tumor samples,Min-iPDX assay showed potential of wide clinical application.Conclusions:Fast in vivo MiniPDX assay based on capsule implantation was developed-to assess drug responses of both PDX tumor grafts and clinical cancer specimens.The high correlation between drug responses of paired MiniPDX and PDX tumor graft assay,as well as translational data suggest that MiniPDX assay is an advanced tool for personalized cancer treatment. | Feifei Zhang Wenjie Wang Yuan Long Hui Liu Jijun Cheng Lin Guo Rongyu Li Chao Meng Shan Yu Qingchuan Zhao Shun Lu Lili Wang Haitao Wang Danyi Wen | 2018 | Cancer Communications2018，38，1： | 20 |
| 9 | Review on supermolecules as chemical drugs显示文摘Supramolecular medicinal chemistry field has been a quite rapidly developing,increasingly active and newly rising interdiscipline which is the new expansion of supramolecular chemistry in pharmaceu-tical sciences,and is gradually becoming a relatively independent scientific area. Supramolecular drugs could be defined as medicinal supermolecules formed by two or more molecules through non-covalent bonds. So far a lot of supermolecules as chemical drugs have been widely used in clinics. Supermolecules as chemical drugs,i.e. supramolecular chemical drugs or supramolecular drugs,which might have the excellences of lower cost,shorter period,higher potential as clinical drugs for their successful research and development,may possess higher bioavailability,better biocompatibility and drug-targeting,fewer multidrug-resistances,lower toxicity,less adverse effect,and better curative effects as well as safety,and therefore exhibit wide potential application. These overwhelming advan-tages have drawn enormous special attention. This paper gives the definition of supramolecular drugs,proposes the concept of supramolecular chemical drugs,and systematically reviews the recent ad-vances in the research and development of supermolecules,including organic and inorganic complex ones as chemical drugs in the area of antitumor,anti-inflammatory,analgesic,antimalarial,antibacterial,antifungal,antivirus,anti-epileptic,cardiovascular agents and magnetic resonance imaging agents and so on. The perspectives of the foreseeable future and potential application of supramolecules as chemical drugs are also presented. | ZHOU ChengHe GAN LinLing ZHANG YiYi ZHANG FeiFei WANG GuangZhou JIN Lei GENG RongXia | 2009 | Science China Chemistry2009，52，4： | 17 |
| 10 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 11 | A GPCR/secretase complex regulates β- and γ-secretase specificity for Aβ production and contributes to AD pathogenesis显示文摘尾 - 地点的 Dysregulation 劈开应用软件的酶(BACE ) 或纬 - secretase 导致异常生产淀粉 -- 尾肽(尾) 并且贡献 Alzheimer 的疾病(广告) 的病原学。自从这些 secretases,调停众多的蛋白质的解朊的处理,因为不可避免的不希望得到的副作用,小成功都没被获得由 secretase 禁止者对待广告。因此,解开具有重要性这些 secretases 的规章的机制。这里,我们证明那未 - opioid 受体(欧洲粪金龟子) 支持由 BACE1 和纬 - 处理尾先锋蛋白质(应用软件) secretase,然而并非槽口, N-cadherin 或 APLP 的。进一步的调查表明那只欧洲粪金龟子与 BACE1 和纬 - secretase 形成建筑群,并且欧洲粪金龟子的激活调停为应用软件 endoproteolysis secretases/receptor 建筑群排序的 co-endocytic。受体的机能障碍延迟 BACE1 和纬 - secretase 并且这样的 endocytosis 尾的生产。一致地,击倒或欧洲粪金龟子的 antagonization 减少 secretase 活动并且改善一个尾病理和 A 尾 - 依赖者行为的赤字,但是不影响在广告模型老鼠处理槽口, N-cadherin 或 APLP。我们的学习不仅揭开为为尾生产调整 secretase 的特性而且建议形成的那干预或 GPCR/secretase 建筑群的 trafficking 的 DOR/secretase 建筑群的形成的分子的机制能对广告导致新策略,潜在地与更少副作用。 | Lin Teng Jian Zhao Feifei Wang Lan Ma Gang Pei | 2010 | Cell Research2010，20，2： | 14 |
| 12 | Pretreatment with Danhong injection protects the brain against ischemia-reperfusion injury显示文摘Danhong injection(DHI),a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii,is widely used in China for treating acute ischemic stroke.In the present study,we explored the neuroprotective efficacy of DHI in a rat model of temporary middle cerebral artery occlusion,and evaluated the potential mechanisms underlying its effects.Pretreatment with DHI(0.9 and 1.8 mL/kg)resulted in a significantly smaller infarct volume and better neurological scores than pretreatment with saline.Furthermore,DHI significantly reduced the permeability of the blood-brain barrier,increased occludin protein expression and decreased neutrophil infiltration,as well as profoundly suppressing the upregulation of matrix metallopeptidase-9 expression seen in rats that had received vehicle.Matrix metallopeptidase-2 expression was not affected by ischemia or DHI.Moreover,DHI(1.8 mL/kg)administered 3 hours after the onset of ischemia also improved neurological scores and reduced infarct size.Our results indicate that the neuroprotective efficacy of DHI in a rat model of cerebral ischemia-reperfusion injury is mediated by a protective effect on the blood-brain barrier and the reversal of neutrophil infiltration. | Shaoxia Wang Hong Guo Xumei Wang Lijuan Chai Limin Hu Tao Zhao Buchang Zhao Xiaoxu Tan Feifei Jia | 2014 | Neural Regeneration Research2014，9，15： | 12 |
| 13 | Effect of high-strength ammonia nitrogen acclimation on sludge activity in sequencing batch reactor显示文摘The effect of high-strength ammonia nitrogen acclimation on sludge activity in sequencing batch reactor (SBR) was investigated. Two batch experiments, RUN1 and RUN2, were conducted with the influent ammonia nitrogen concentrations 60 and 500 mg/L, respectively. The sludges inoculated from RUN1 and RUN2 were used to treat a series of influent with ammonia nitrogen concentrations of 59, 232, 368, 604 and 1152 mg/L. It is found that the activated sludge acclimated to higher ammonia nitrogen concentrations revealed higher COD and NH 4 + -N removal efficiencies, and slower DHA decrease. The results confirmed that the activities of the bacteria in activated sludge in SBR were inhibited by high-strength ammonia nitrogen, whereas the activated sludge acclimated to high-strength ammonia nitrogen showed substantial resistance to inhibition by influents containing high levels of ammonia nitrogen. | Feifei Wang, Yuanhong Ding, Lei Ge, Hongqiang Ren, Lili Ding State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China. | 2010 | Journal of Environmental Sciences2010，22，11： | 12 |
| 14 | Secondary Crystallization of Na_2CO_3-Modified HZSM-5 Zeolites with Tetrapropylammonium Hydroxide and Their Catalytic Performance in Thiophene Alkylation Reaction显示文摘The Na2CO3-modified HZSM-5 zeolites were further treated by tetrapropylammonium hydroxide(TPAOH) solution. The effect of TPAOH concentration on the secondary crystallization process was investigated. The resulting samples were characterized by a complementary combination of X-ray diffraction, N2 adsorption/desorption, scanning electron microscopy, X-ray fluorescence spectroscopy, XPS, 27 Al and 29 Si magic-angle spinning nuclear magnetic resonance spectroscopy, BET and temperature-programmed desorption techniques. The results showed that the secondary crystallization of the HZSM-5 zeolite could result in migration of non-framework species from the internal channels to the zeolite surface and their transformation into framework species. The catalytic activity of these modified samples for thiophene alkylation was evaluated. Both the activity and stability of the catalysts were improved after secondary crystallization. | Liu Dongmei Kong Feifei Zhai Yuchun Wang Haiyan | 2015 | China Petroleum Processing & Petrochemical Technology2015，17，3： | 10 |
| 15 | Paleomagnetic results of Late Paleozoic rocks from northern Qiangtang Block in Qinghai-Tibet Plateau, China显示文摘Tectonic evolution of the Tethys and the boundary between the Gondwanaland and the Eurasia during the Carboniferous and Permian remain hotly debated. Qiangtang region in the Qinghai-Tibet Plateau may be a key place to study these problems. A paleomagnetic study was conducted on the Late Paleozoic rocks in the northern Qiangtang region (33.7°N, 86.7°W), Tibet. Two sites (21 samples) in the Upper Carboniferous, eleven sites (101 samples) in the Permian, and two sites (16 samples) in the Lower Triassic were investigated. The rock magnetic data revealed hematite and magnetite as the main magnetic carriers. In stepwise thermal demagnetization and/or combined alternating field (AC) demagnetization, two characteristic components in the majority of the samples were identified as (1) the Low-temperature Component (LTC), characterized by northerly decli- nation and moderate to steep inclination, corresponding to a pole position overlay with the present North Pole. A minority of the samples present single component, and their directions are the same as (2) the High-temperature Component (HTC) of double components. The combined single-component and HTC data of the Permian can pass the R-test at 95% level and the F-test at 99% level, as well as the BC-test. The pole position from the Late Carboniferous is at 31.8°S, 45.7°E with dp=2.1, dm=3.9, that from the Early and Middle (Late) Permian is at 31.7°S, 46.8°E with dp=9.2, dm=16.9 (34.4°N, 54.1°E with dp=6.9, dm=12.5) respectively, and that from the Early Triassic is at 16.9°S, 22.5°E with dp=4.9, dm=9.2. These pole positions are different from the other poles for the Qiangtang Block, which suggests the single-component and HTC directions are probably a primary magnetization and the northern Qiangtang Block was paleogeographically situated at low latitudes in the Northern Hemisphere in the Late Paleozoic. | CHENG Xin WU HanNing GUO Qiang HOU BaoNing XIA LingYan WANG HaiJun DIAO ZongBao HUO FeiFei JI WenHua LI RongShe CHEN ShouJian ZHAO ZhenMing LIU XiaoJi | 2012 | Science China Earth Sciences2012，55，1： | 9 |
| 16 | Wnt/β-catenin signaling in cancers and targeted therapies显示文摘Wnt/β-catenin signaling has been broadly implicated in human cancers and experimental cancer models of animals.Aberrant activation of Wnt/β-catenin signaling is tightly linked with the increment of prevalence,advancement of malignant progression,development of poor prognostics,and even ascendence of the cancer-associated mortality.Early experimental investigations have proposed the theoretical potential that efficient repression of this signaling might provide promising therapeutic choices in managing various types of cancers.Up to date,many therapies targeting Wnt/β-catenin signaling in cancers have been developed,which is assumed to endow clinicians with new opportunities of developing more satisfactory and precise remedies for cancer patients with aberrant Wnt/β-catenin signaling. | Fanyuan Yu Changhao Yu Feifei Li Yanqin Zuo Yitian Wang Lin Yao Chenzhou Wu Chenglin Wang Ling Ye | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 8 |
| 17 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 18 | Measurement-based Frequency Dynamic Response Estimation Using Geometric Template Matching and Recurrent Artificial Neural Network显示文摘Understanding power system dynamics after an event occurs is essential for the purpose of online stability assessment and control applications.Wide area measurement systems(WAMS)based on synchrophasors make power system dynamics visible to system operators,delivering an accurate picture of overall operating conditions.However,in actual field implementations,some measurements can be inaccessible for various reasons,e.g.,most notably communication failure.To reconstruct these inaccessible measurements,in this paper,the radial basis function artificial neural network(RBF-ANN)is used to estimate the system dynamics.In order to find the best input features of the RBF-ANN model,geometric template matching(GeTeM)and quality-threshold(QT)clustering are employed from the time series analysis to compute the similarity of frequency dynamic responses in different locations of the power system.The proposed method is tested and verified on the Eastern Interconnection(EI)transmission system in the United States.The results obtained indicate that the proposed approach provides a compact and efficient RBF-ANN model that accurately estimates the inaccessible frequency dynamic responses under different operating conditions and with fewer inputs. | Feifei Bai Xiaoru Wang Yilu Liu Xinyu Liu Yue Xiang Yong Liu | 2016 | CSEE Journal of Power and Energy Systems2016，2，3： | 8 |
| 19 | Xenograft tumors derived from malignant pleural effusion of the patients with non-small-cell lung cancer as models to explore drug resistance显示文摘Background:Non-small cell lung cancer(NSCLC)patients with epidermal growth factor receptor(EGFR)mutations or anaplastic lymphoma kinase(ALK)fusions show dramatic responses to specific tyrosine kinase inhibitors(TKIs);however,after 10-12 months,secondary mutations arise that confer resistance.We generated a murine xenograft model using patient-derived NSCLC cells isolated from the pleural fluid of two patients with NSCLC to investigate the mechanisms of resistance against the ALK-and EGFR-targeted TKIs crizotinib and osimertinib,respectively.Methods:Genotypes of patient biopsies and xenograft tumors were determined by whole exome sequencing(WES),and patients and xenograft-bearing mice received targeted treatment(crizotinib or osimertinib)accordingly.Xenograft mice were also treated for prolonged periods to identify whether the development of drug resistance and/or treatment responses were associated with tumor size.Finally,the pathology of patients biopsies and xenograft tumors were compared histologically.Results:The histological characteristics and chemotherapy responses of xenograft tumors were similar to the actual patients.WES showed that the genotypes of the xenograft and patient tumors were similar(an echinoderm microtu-bule-associated protein-like 4-ALK(EML4-ALK)gene fusion(patient/xenograft:CTC15035EML4-ALK)and EGFR L858R and T790M mutations(patient/xenograft:CTC15063EGFR L858R,T790M)).After continuous crizotinib or osimertinib treatment,WES data suggested that acquired ALK E1210K mutation conferred crizotinib resistance in the CTC15035EML4-ALK xenograft,while decreased frequencies of EGFR L858R and T790M mutations plus the appearance of v-RAF murine sarcoma viral oncogene homolog B(BRAF)G7V mutations and phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha(PIK3C2A)A86fs frame shift mutations led to osimertinib resistance in the CTC15063EGFR L858R,T790M xenografts.Conclusions:We successfully developed a new method of generating drug resistance xenograft models from liquid biopsies using microfluidic technology,which might be a useful tool to investigate the mechanisms of drug resist-ance in NSCLC. | Yunhua Xu Feifei Zhang Xiaoqing Pan Guan Wang Lei Zhu Jie Zhang Danyi Wen Shun Lu | 2018 | Cancer Communications2018，38，1： | 7 |
| 20 | A lentiviral vector-based therapeutic vaccine encoding Ag85B-Rv3425 potently increases resistance to acute tuberculosis infection in mice显示文摘为 multidrug 抵抗的肺结核(TB ) 和到为 TB 的新奇治疗学的途径的发展的广泛地药抵抗的 TB 电话注意的很少处理选择。因为他们能导致抗原特定的细胞的有免疫力的回答,治疗学的疫苗正在答应候选人,它在 Mycobacterium 肺结核(鱼雷快艇) 的消除起一个重要作用。在这研究,新奇 lentiviral 向量为交付鱼雷快艇特定的熔化蛋白质 Ag85B-Rv3425 的治疗学的疫苗被构造。结果证明这支 recombinant lentivirus 疫苗单个注射的能在老鼠触发抗原特定的 Th1 类型有免疫力的回答。更重要地,有急性感染的老鼠在肺和脾由显著地减少的鱼雷快艇负担从这支疫苗的一个单个剂量的政府有益于很多以及与未经治疗的老鼠相比在肺稀释了损害。这些结果为 TB 的免疫疗法显示了这支新奇疫苗的好前景。 | Enzhuo Yang Feifei Wang Ying Xu Honghai Wang Yong Hu Hongbo Shen Zheng W. Chen | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 6 |
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