|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway显示文摘Epalrestat is a noncompetitive and reversible aldose reductase inhibitor used for the treatment of diabetic neuropathy. This study assumed that epalrestat had a protective effect on diabetic peripheral nerve injury by suppressing the expression of aldose reductase in peripheral nerves of diabetes mellitus rats. The high-fat and high-carbohydrate model rats were established by intraperitoneal injection of streptozotocin. Peripheral neuropathy occurred in these rats after sustaining high blood glucose for 8 weeks. At 12 weeks after streptozotocin injection, rats were intragastrically administered epalrestat 100 mg/kg daily for 6 weeks. Transmission electron microscope revealed that the injuries to myelinated nerve fibers, non-myelinated nerve fibers and Schwann cells of rat sciatic nerves had reduced compared to rats without epalrestat administuation. Western blot assay and immunohistochemical results demonstrated that after intervention with epalrestat, the activities of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase gradually increased, but aldose reductase protein expression gradually diminished. Results confirmed that epalrestat could protect against diabetic peripheral neuropathy by relieving oxidative stress and suppressing the polyol pathway. | Qing-rong Li Zhuo Wang Wei Zhou Shou-rui Fan Run Ma Li Xue Lu Yang Ya-shan Li Hong-li Tan Qi, ng-hua Shao Hong-ying Yang | 2016 | Neural Regeneration Research2016，11，2： | 42 |
| 2 | The Sunway TaihuLight supercomputer: system and applications显示文摘The Sunway Taihu Light supercomputer is the world's first system with a peak performance greater than 100 PFlops. In this paper, we provide a detailed introduction to the Taihu Light system. In contrast with other existing heterogeneous supercomputers, which include both CPU processors and PCIe-connected many-core accelerators(NVIDIA GPU or Intel Xeon Phi), the computing power of Taihu Light is provided by a homegrown many-core SW26010 CPU that includes both the management processing elements(MPEs)and computing processing elements(CPEs) in one chip. With 260 processing elements in one CPU, a single SW26010 provides a peak performance of over three TFlops. To alleviate the memory bandwidth bottleneck in most applications, each CPE comes with a scratch pad memory, which serves as a user-controlled cache. To support the parallelization of programs on the new many-core architecture, in addition to the basic C/C++and Fortran compilers, the system provides a customized Sunway Open ACC tool that supports the Open ACC2.0 syntax. This paper also reports our preliminary efforts on developing and optimizing applications on the Taihu Light system, focusing on key application domains, such as earth system modeling, ocean surface wave modeling, atomistic simulation, and phase-field simulation. | Haohuan FU Junfeng LIAO Jinzhe YANG Lanning WANG Zhenya SONG Xiaomeng HUANG Chao YANG Wei XUE Fangfang LIU Fangli QIAO Wei ZHAO Xunqiang YIN Chaofeng HOU Chenglong ZHANG Wei GE Jian ZHANG Yangang WANG Chunbo ZHOU Guangwen YANG | 2016 | Science China(Information Sciences)2016，59，7： | 45 |
| 3 | A Novel QTL qTGW3 Encodes the GSK3/ SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice显示文摘谷物尺寸和形状在米饭是谷物重量和产量的重要决定因素。这里,我们报导一个新主要量的特点在米饭的地点(QTL ) , qTGW3,那种控制谷物尺寸和重量。这个地点, qTGW3,编码 OsSK41 (也作为 OsGSK5 知道) , GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like 家庭的一个成员。带 OsSK41 的 loss-of-function 等位基因的瑞斯 near-isogenic 线增加了谷物长度和重量。我们证明那 OsSK41 交往与并且 phosphorylates 植物生长素反应因素 4 (OsARF4 ) 。有 OsARF4 的 OsSK41 的合作表示在米饭原物增加 OsARF4 的累积。OsARF4 的功能的损失导致更大的米饭谷物。定序 RNA 分析建议 OsARF4 和 OsSK41 镇压下游的基因的一个普通集合的表示, ? 包括一些植物生长素应答的基因,在米饭谷物开发期间。在 qTGW3 的 OsSK41 的 loss-of-function 形式代表没被指向的基因编辑或 QTL 节节上升广泛地在 OsSK41 功能的米饭 breeding.?Suppression 利用了的稀罕等位基因提高米饭谷物尺寸和重量。因此,我们的学习在米饭谷物开发揭示 OsSK41 的重要角色 ?? 并且在另外的谷物庄稼在米饭并且也许为谷物产量的基因改进提供新候选人基因。 | Zejun Hu Sun-Jie Lu Mei-Jing Wang Haohua He Le Sun Hongru Wang Xue-Huan Liu Ling Jiang Jing-Liang sun Xiaoyun Xin Wei Kong Chengcai Chu Hong-Wei Xue Jinshui Yang Xiaojin Luo Jian-Xiang Liu | 2018 | Molecular Plant2018，11，5： | 47 |
| 4 | Design and engineering implementation of non-supplementary fired compressed air energy storage system: TICC-500显示文摘The integration and accommodation of the wind and solar energy pose great challenges on today’s power system operation due to the intermittent nature and volatility of the wind and solar resources.High efficient large-scale electrical energy storage is one of the most effective and economical solutions to those problems.After the comprehensive review of the existing storage technologies,this paper proposes an overall design scheme for the Non-supplementary Fired Compressed Air Energy Storage(NFCAES)system,including system design,modeling and efficiency assessment,as well as protection and control.Especially,the design principles of the multistage regenerative,i.e.heat recovery system which is used to fully recycle and utilize the waste heat from compression are provided,so as the overall system efficiency evaluation method.This paper theoretically ascertains the storage decoupling rules in the potential and internal energy of molecular compressed air and reveals the conversion mechanism of gas,heat,power,electricity and other forms of energy.On this basis,a 500-k W physical simulation system of CAES system(TICC-500,Tsinghua-IPCCAS-CEPRI-CAES)is built,which passed a system-wide 420-k W load power generation test with less pollution and zero carbon emissions.Besides,the multi-form energy conversion of multi-stage regenerative CAES and storage efficiency is verified,especially its incomparable superiority in solving the uncertainty problem in wind and solar power generation.Finally,the propaganda and application scenario of the CAES system in China is introduced. | MEI Sheng Wei WANG Jun Jie TIAN Fang CHEN Lai Jun XUE Xiao Dai LU Qiang ZHOU Yuan ZHOU Xiao Xin | 2015 | Science China(Technological Sciences)2015，58，4： | 37 |
| 5 | Oral health status in Sichuan Province: findings from the oral health survey of Sichuan, 2015–2016显示文摘To investigate oral health status in the residents of Sichuan Province, southwest China, a cross-sectional study was performed using the latest Oral Health Survey Basic Methods recommended by the World Health Organization. A multistage stratified random cluster-sampling method was used to enroll participants from the following three groups: children aged 3–5 years,adolescents aged 12 years, and people aged 65–74 years. In these three groups, the mean numbers of teeth that were affected by caries were 3.28, 0.86 and 5.13, respectively, resulting in a prevalence of 63.47%, 37.20% and 83.20%, respectively.Relative to the high rate of decayed teeth, the prevalence of fillings was very low in all age groups(0.97%, 7.24% and 5.43%,respectively). In the 12-year-old adolescent group, only 3.61% had good pit and fissure sealing. In addition, the rate of dental fluorosis was 24.80%, and the Community Fluorosis Index value was 0.39. In the elder group, the community periodontal index was2.92. The prevalence in the elderly of having lost at least one tooth was 75.54%. Additionally, 4.44% of these participants were edentulous. The incidence of dental prosthesis was 51.75%, the proportion with a removable partial denture, a fixed denture, full dentures, dental implants and an informal fixed bridge was 21.59%, 11.45%, 4.64%, 0 and 16.67%, respectively. In this study,8.2% of the elderly participants were affected by different types of oral mucosal lesions. Among such lesions, recurrent aphthous ulcers were most prevalent(2.69%) and oral lichen planuses were second(1.6%). The conclusion presented in this survey is that oral diseases, especially dental caries and periodontal disease, are frequent and common in Sichuan province, China. Moreover, the treatment rate is very low, and primary prevention and treatment options are therefore urgently needed in this population. | Wei Yin Ying-Ming Yang Hong Chen Xue Li Zhuo Wang Li Cheng Qiu-Dan Yin Hong-Zhi Fang Wei Fei Fang-Lin Mi Min-Hai Nie Tao Hu Xue-Dong Zhou | 2017 | International Journal of Oral Science2017，9，1： | 30 |
| 6 | Study of spatial distribution of sandy desertification in North China in recent 10 years显示文摘Sandy desertification is a land degradation characterized by wind erosion, mainly resulted from the excessive human activities in arid, semiarid and part of sub-humid regions in North China. It is one of main kinds of desertification/land degradation as well as water-soil erosion and salinization in China. Rapid and continuous spread of sandy desertification during last 50 years has created a major environmental and socio-economic problem in North China. Remote sensing monitored results in 2000 showed that the sandy desertified land area has been 38.57×104 km2. The area of potential to slightly sandy desertified land is 13.93×104 km2, moderately land 9.977×104 km2, severely land 7.909×104 km2 and very severely land 6.756×104km2. Sandy desertification mainly occurs in the semi-arid mixed farming-grazing zone and its northern rangeland zone, semi-arid dryland rainfed cropping zone and arid oasis-desert margin zone. The average annually developmental rate of sandy desertified land increased from 2,100tion in North China is 'overall deterioration, while local rehabilitation'. Already achieved rehabilitation results and monitoring assessment show that about 60% of desertified land in North China can be restored under the conditions of rational land-use ways and intensity. | WANG Tao, WU Wei, XUE Xian, SUN Qingwei & CHEN GuangtingKey Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China Department of Environment Engineering, Peking University, Beijing 100871, China | 2004 | Science China Earth Sciences2004，47，z1： | 28 |
| 7 | Development of Oryza rufipogon and O. sativa Introgression Lines and Assessment for Yield-related Quantitative Trait Loci显示文摘基因渗入线人口有效地在印射量的特点 loci (QTL ) 被使用,识别有利基因,发现隐藏的遗传变异,评估处于多重条件的 QTL 的行动或相互作用并且提供为植物繁殖和基因研究的有利试验性的材料。在这研究,先进回交和连续 selfing 策略被用来开发基因渗入线(IL ) ,它源于 Oryza rufipogon Griff 的就职。从 Yuanjiang 县镇定,中国的云南省,著名计算机生产厂商他施主,和精英 indica 栽培变种 Teqing (O。sativa L.) ,接受者。从 O 的 Introgressionsegments。rufipogon 用 179 次多态的简单顺序重复(SSR ) 被屏蔽在每 IL 的染色体的标记。基因渗入线人口带的 Introgressed 片断包含了盖住整个 O 的 120 IL。rufipogon 染色体。同型结合的 O 的吝啬的数字。rufipogonsegments 每基因渗入线是大约 3.88。introgressed 片断的平均长度是近似的 25.5 厘米,并且有的大约 20.8% 这些片断缩放不到 10 厘米。eachIL 的染色体怀有 O 的 chromosomal 碎片。rufipogon 从 0.54% ~ 23.7%,与 5.79% 的全面一般水准。在每个地点, O 的替换的比率。rufipogon 等位基因有范围 of1.67-9.33,与 5.50 的一般水准。大量改变在词法并且 yield-relatedtraits 也在基因渗入线人口被发现。用单个点的分析,为产量和产量构成因素的 37 通常认为的 QTL 的一个总数与解释 phenotypic 的 7%-20% 在二个地点被检测。十九 QTL (51.4%) 在两个地点被检测,并且从在十五 loci (40.5%) 的 O.rufipogon 的等位基因在 Teging 背景改进了收益和产量构成因素。这些 O。rufipogon-O。sativa 基因渗入线将为从普通野米饭识别并且使用有利基因用作遗传物质…… | Lubin Tan | 2007 | Journal of Integrative Plant Biology2007，49，6： | 28 |
| 8 | Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor显示文摘 | Lijie Wang Wei Xue Lei Yan Xiaosa Li Jia Wei Miaomiao Chen Jing Wu Bei Yang Li Yang Jia Chen | 2017 | Cell Research2017，27，10： | 26 |
| 9 | Differentiation of isolated human umbilical cord mesenchymal stem cells into neural stem cells显示文摘AIM:To investigate whether umbilical cord human mesenchymal stem cell(UC-MSC)was able to differentiate into neural stem cell and neuron in vitro.·METHODS:The umbilical cords were obtained from pregnant women with their written consent and the approval of the Clinic Ethnics Committee.UC-MSC were isolated by adherent culture in the medium contains 20%fetal bovine serum(FBS),then they were maintained in the medium contain 10%FBS and induced to neural cells in neural differentiation medium.We investigated whether UC-MSC was able to differentiate into neural stem cell and neuron in vitro by using flow cytometry,reverse transcriptase-polymerase chain reaction(RT-PCR)and immunofluorescence(IF)analyzes.·RESULTS:A substantial number of UC-MSC was harvested using the tissue explants adherent method at about 2wk.Flow cytometric study revealed that these cells expressed common markers of MSCs,such as CD105(SH2),CD73(SH3)and CD90.After induction of differentiation of neural stem cells,the cells began to form clusters;RT-PCR and IF showed that the neuron specific enolase(NSE)and neurogenic differentiation 1-positive cells reached 87.3%±14.7%and 72.6%±11.8%,respectively.Cells showed neuronal cell differentiation after induced,including neuron-like protrusions,plump cell body,obviously and stronger refraction.RT-PCR and IF analysis showed that microtubule-associated protein 2(MAP2)and nuclear factor-M-positive cells reached 43.1%±10.3%and 69.4%±19.5%,respectively.·CONCLUSION:Human umbilical cord derived MSCscan be cultured and proliferated in vitro and differentiate into neural stem cells,which may be a valuable source for cell therapy of neurodegenerative eye diseases. | Song Chen Wei Zhang Ji-Ming Wang Hong-Tao Duan Jia-Hui Kong Yue-Xin Wang Meng Dong Xue Bi Jian Song | 2016 | International Journal of Ophthalmology(English edition)2016，9，1： | 22 |
| 10 | Exosomes containing miR-21 transfer the characteristic of cisplatin resistance by targeting PTEN and PDCD4 in oral squamous cell carcinoma显示文摘 | Tao Liu Gang Chen Dawei Sun Minghui Lei Yongqiang Li Changming Zhou Xiaodong Li Wei Xue Hong Wang Chunjun Liu Jiang Xu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，9： | 22 |
| 11 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 12 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 13 | The Yanshan orogeny and late Mesozoic multi-plate convergence in East Asia—Commemorating 90th years of the “Yanshan Orogeny”显示文摘The Yanshan movement/orogeny has been proposed for 90 years, which is of special significance in the history of geological research in China. This study conducted a review by synthesizing major achievements regarding episodic deformation features, sedimentary and magmatic records of the Yanshan orogeny in China, and clarified the episodic tectono-magmatism and its geodynamic origins. The tectonic implications of the Yanshan orogeny are discussed in the context of global plate tectonics and supercontinent reconstruction. Lines of evidence from structural, sedimentary and magmatic data suggest that the Yanshan orogeny represents a regional-scale tectonic event that affected the entire China continent in late Mesozoic period. Numerous age and structural constraints consistently indicate that the Yanshan orogeny was initiated in the Jurassic(at ~170±5 Ma). and was characterized by alternating stages of crustal shortening at ~170–136 Ma, crustal extension at ~135–90 Ma, and weak shortening at ~80 Ma. The 170–136 Ma crustal shortening was reflected in the generation of two regional stratigraphic unconformities(the Tiaojishan and Zhangjiakou unconformities), which were initially named the A and B episodes of 'the Yanshan Orogeny' by Mr.Wong Wenhao in 1928. Geodynamically, the Yanshan orogeny in East Asia was associated with nearly coeval oceanic subduction and continental convergence in the Paleo-Pacific, Neo-Tethys, and Mongol-Okhotsk tectonic domains. As a consequence, three giant accretionary-collisional tectonic systems were formed along the continental margins of East Asia, i.e., the Mongol-Okhotsk, Bangonghu-Nujiang, and SE China subduction-and collision-related accretionary systems. The Yanshan orogeny induced widespread crustal-scale folding and thrusting, tectonic reactivation of long-lived zones of crustal weakness,and extensive magmatism and mineralization in intraplate regions. Based on the time principle of supercontinent assembly and break-up, we propose that the mid-Late Jurassic multi-plate convergence in East Asia might represent the initiation of the assembly of the Amasia supercontinent, and the Yanshan orogeny might be the first 'stirrings' that is a prerequisite for the birth of the Amasia supercontinent. | Shuwen DONG Yueqiao ZHANG Hailong LI Wei SHI Huaimin XUE Jianhua LI Shiqi HUANG Yongchao WANG | 2018 | Science China Earth Sciences2018，61，12： | 21 |
| 14 | Experimental research on overlying strata movement and fracture evolution in pillarless stress-relief mining显示文摘 | Junhua Xue Hanpeng Wang Wei Zhou Bo Ren Changrui Duan Dongsheng Deng | 2015 | International Journal of Coal Science & Technology2015，2，1： | 21 |
| 15 | Variation of head and facial morphological characteristics with increased age of Han in Southern China显示文摘We investigated 13940 (6735 male, 7250 female) adult head and facial physical attributes from 19 different Han ethnic groups in 10 southern-China provinces, and calculated 12 head and facial indexes. Indexes were used to analyze the variation of head and facial morphological characteristics with increased age. Results showed that as age increases: (1) Head breadth, minimum frontal breadth, face breadth, interocular breadth, external biocular breadth, lip height, lip thickness, head circumference, auricular height, length-breadth head index, length-height head index, and lip-index values decline significantly in a linear fashion. (2) Nose breadth, mouth breadth, morphological facial height, upper-lip height, physiognomic ear length, physiognomic ear breadth, visor skin-fold, and vertical head-facial index values significantly increase in a linear fashion. | LI YongLan ZHENG LianBin YU KeLi LU ShunHua ZHANG XingHua LI YuLing WANG Yang XUE Hong DENG Wei | 2013 | Chinese Science Bulletin2013，58，4： | 20 |
| 16 | Infection with novel coronavirus(SARS-CoV-2)causes pneumonia in Rhesus macaques显示文摘The 2019 novel coronavirus(SARS-CoV-2)outbreak is a major challenge for public health.SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases,with a mortality rate of^6.4%worldwide(based on World Health Organization daily situation report).However,the dynamics of viral infection,replication and shedding are poorly understood.Here,we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2.After intratracheal inoculation,the first peak of viral RNA was observed in oropharyngeal swabs one day post infection(1 d.p.i.),mainly from the input of the inoculation,while the second peak occurred at 5 d.p.i.,which reflected on-site replication in the respiratory tract.Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals,characterized by hyperemia and edema,and infiltration of monocytes and lymphocytes in alveoli.We also identified SARS-CoV-2 RNA in respiratory tract tissues,including trachea,bronchus and lung;and viruses were also re-isolated from oropharyngeal swabs,bronchus and lung,respectively.Furthermore,we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2.The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics. | Chao Shan Yan-Feng Yao Xing-Lou Yang Yi-Wu Zhou Ge Gao Yun Peng Lian Yang Xue Hu Jin Xiong Ren-Di Jiang Hua-Jun Zhang Xiao-Xiao Gao Cheng Peng Juan Min Ying Chen Hao-Rui Si Jia Wu Peng Zhou Yan-Yi Wang Hong-Ping Wei Wei Pang Zheng-Fei Hu Long-Bao Lv Yong-Tang Zheng Zheng-Li Shi Zhi-Ming Yuan | 2020 | Cell Research2020，30，8： | 19 |
| 17 | Wnt and BMP signaling crosstalk in regulating dental stem cells:Implications in dental tissue engineering显示文摘Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling.Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance,essential oral functions and the quality of life.Regenerative dentistry holds great promise in treating oral/dental disorders.The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells,along with the signaling mechanisms governing stem cell self-renewal and differentiation.In this review,we first summarize the biological characteristics of seven types of dental stem cells,including dental pulp stem cells,stem cells from apical papilla,stem cells from human exfoliated deciduous teeth,dental follicle precursor cells,periodontal ligament stem cells,alveolar bone-derived mesenchymal stem cells(MSCs),and MSCs from gingiva.We then focus on how these stem cells are regulated by bone morphogenetic protein(BMP)and/or Wnt signaling by examining the interplays between these pathways.Lastly,we analyze the current status of dental tissue engineering strategies that utilize oral/dental stem cells by harnessing the interplays between BMP and Wnt pathways.We also highlight the challenges that must be addressed before the dental stem cells may reach any clinical applications.Thus,we can expect to witness significant progresses to be made in regenerative dentistry in the coming decade. | Fugui Zhang Jinlin Song Hongmei Zhang Enyi Huang Dongzhe Song Viktor Tollemar Jing Wang Jinhua Wang Maryam Mohammed Qiang Wei Jiaming Fan Junyi Liao Yulong Zou Feng Liu Xue Hu Xiangyang Qu Liqun Chen Xinyi Yu Hue H.Luu Michael J.Lee Tong-Chuan He Ping Ji | 2016 | Genes & Diseases2016，3，4： | 16 |
| 18 | Droplet deposition and efficiency of fungicides sprayed with small UAV against wheat powdery mildew显示文摘Small unmanned aerial vehicle(UAV),a timely and effective pesticide sprayer,can be used to spray pesticide in a high efficiency without harming the crop,and is especially suitable for spraying for small farms and hills in Asian countries.In recent years,it has been chosen as a key and new technique for pesticide application.This paper studied the impact of UAV(UAV N-3)spraying parameters(different working height and different spraying concentrations)on the deposition of droplets on the wheat canopy and the prevention of powdery mildew.Water sensitive paper was used as the sampler to collect the pesticide droplets and image processing software Deposit Scan was used to compute the coverage rate of droplets on the wheat top layer to acquire the proportional distribution of droplets on the wheat lower layer.The experimental results showed the impact of spraying height on the distribution of droplets on the wheat upper layer was quite significant,when the spraying height was 5.0 m and the spraying speed was 4 m/s,the coverage rate of droplets on the wheat lower layer was the largest,as it was 45.6%of that on the upper layer,the droplets distribution was the most uniform,and the coefficient of variation was 33.13%.450 g/hm^(2)(dosage registered)of triadimefon SC(44%)was sprayed by the UAV,the control efficiency reached 55.1%after applying which was better than 20%and 40%of dosage decreased for each hectare,and the applying effect(35.6%)of 20%dosage decreased for each hectare had no significant difference from the applying effect(34.6%)applied by a knapsack-type electric sprayer.At 10 d after applying,the prevention effect realized by UAV was lower than that realized by a knapsack-type electric sprayer,and it may be correlated to the meteorological condition and water amount in the pesticide sprayed.Thus,when UAV spraying was chosen to prevent wheat powdery mildew under a serious disease situation,an auxiliary agent for spraying could be added to prolong the retention of pesticide on the plant surface to extend the pesticide effect.This study can provide a reference for the optimized design,performance upgrade and reasonable application of small UAV sprayers. | Weicai Qin Xinyu Xue Shaoming Zhang Wei Gu Baokun Wang | 2018 | International Journal of Agricultural and Biological Engineering2018，11，2： | 16 |
| 19 | Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients显示文摘BACKGROUND The methylated septin 9(mSEPT9) assay was the first blood-based test approved by the United States Food and Drug Administration as a colorectal screening test.However, the diagnostic and prognostic role of preoperative mSEPT9 for colorectal cancer(CRC) in Chinese patients is still unknown.AIM To improve the understanding of diagnostic and prognostic factors, serum m SEPT9 was detected in Chinese CRC patients.METHODS A retrospective analysis of 354 cases, of which 300 had CRC and 54 were normal,was performed in China. Patients' characteristics, treatments, and laboratory data, including age, the date of surgery, Union for International Cancer Control(UICC) stages, distant metastasis(M), and so on, were collected. Methylation levels of SEPT9 were quantified by quantitative, methylation-specific polymerase chain reaction before surgery. In addition, the effects of mSEPT9 on the occurrence and prognosis of 330 CRC cases from The Cancer Genome Atlas(TCGA) database were evaluated using bioinformatics analyses. Potential prognostic factors for overall survival(OS) and progression-free survival(PFS)were evaluated by Kaplan-Meier univariate analysis.RESULTSIn Chinese CRC patients, positive mSEPT9 was strongly associated with advanced UICC stages, deeper invasion by the primary tumor, and more distant metastasis. Methylation levels of SEPT9 were stage-dependent and showed a stepwise increase in UICC stages(I–IV), primary tumor categories(T1–T4),regional node categories(N0–N2), and distant metastasis categories(M0–M1).The patients with positive mSEPT9 showed a tendency toward lower PFS. After analyzing TCGA clinical data, the high mSEPT9 group was found to be obviously correlated only with more distant metastasis. The patients with high mSEPT9 levels showed a tendency toward lower OS. Besides, nine meaningful mSEPT9 sites were found to provide guidance for the follow-up studies.CONCLUSION MSEPT9 analysis may add valuable information to current tumor staging. Serum m SEPT9 in Chinese CRC patients appears to offer promising novel prognostic markers and might be considered for monitoring CRC recurrence. | Xue Yang Zhi-Jie Xu Xi Chen Shuang-Shuang Zeng Long Qian Jie Wei Mei Peng Xiang Wang Wan-Li Liu Hong-Ying Ma Zhi-Cheng Gong Yuan-Liang Yan | 2019 | World Journal of Gastroenterology2019，25，17： | 16 |
| 20 | Ethnopharmacology, Phytochemistry, Pharmacology, Toxicology and Clinical Applications of Radix Astragali显示文摘Radix Astragali(RA),a traditional Chinese medicine from the dried root of Astragalus species,is widely distributed throughout the temperate regions of the world.The major bioactive constituents of RA are triterpene glycosides,flavonoids,saponins,and alkaloids,and these compounds mostly exert pharmacological activities on the cardiovascular,immune,respiratory,and hepatic systems.This review summarizes the recent studies on RA and provides a comprehensive summary regarding the status of resources,ethnopharmacology,phytochemistry,pharmacology,toxicology,clinical application,and patent release of RA.We hope this review can provide a guidance for further development of therapeutic agents from RA. | ZHANG Chun-hong YANG Xiao WEI Jing-ran CHEN Na-mu-han XU Jian-ping BI Ya-qiong YANG Min GONG Xue LI Zi-yan REN Kai HAN Qi-heng ZHANG Lei LI Xue JI Ming-yue WANG Cong-cong LI Min-hui | 2021 | Chinese Journal of Integrative Medicine2021，27，3： | 16 |
您所在的机构暂未开通此部分的功能，请联系管理员