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| 1 | PROTAC-induced BTK degradation as a novel therapy for mutated BTK C481S induced ibrutinib-resistant B-cell malignancies显示文摘 | Yonghui Sun Xingwang Zhao Ning Ding Hongying Gao Yue Wu Yiqing Yang Meng Zhao Jinseok Hwang Yuqin Song Wanli Liu Yu Rao | 2018 | Cell Research2018，28，7： | 29 |
| 2 | Enhanced delivery of hydrophilic peptides in vitro by transdermal microneedle pretreatment显示文摘The aims of this study were to investigate the utility of solid microneedle arrays(150 mm in length)in enhancing transdermal delivery of peptides and to examine the relationship between peptide permeation rates and D_(2)O flux.Four model peptides were used(Gly-Gln-Pro-Arg[tetrapeptide-3,456.6 Da],Val-Gly-Val-Ala-Pro-Gly[hexapeptide,498.6 Da],AC-Glu-Glu-Met-Gln-Arg-Arg-NH2[acetyl hexapeptide-3,889 Da]and Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2[oxytocin,1007.2 Da]).The influence of microneedle pretreat-ment on skin permeation was evaluated using porcine ear skin with Franze diffusion cell.Peptide permeation across the skin was significantly enhanced by microneedle pretreatment,and permeation rates were dependent on peptide molecular weights.A positive correlation between D_(2)O flux and acetyl hexapeptide-3 clearances suggests that convective solvent flow contributes to the enhanced transdermal peptide delivery.It is concluded that solid microneedle arrays are effective devices to enhance skin delivery of peptides. | Suohui Zhang Yuqin Qiu Yunhua Gao | 2014 | Acta Pharmaceutica Sinica B2014，4，1： | 6 |
| 3 | Observations of nonlinear oscillation phenomena in metallic solids显示文摘A great number of in situ observations proved that at normal temperature and pressure there exists a nonlinear oscillation phenomenon in the solid alloys. This kind of oscillation phenomenon exhibits fluid-like character in localized areas, fluctuating with crystal rotation, cell’s moving, expansion and contraction. | Houxiu Gao Yuqin Yang Jingwu Yang Anwei Liu | 1999 | Chinese Science Bulletin1999，44，1： | 6 |
| 4 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 5 | Methionine deficiency and its hydroxy analogue influence chicken intestinal 3-dimensional organoid development显示文摘Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal regeneration.The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal development.Here,we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore the influence of methionine deficiency and MHA on intestinal organoid development.The results showed that 125-mm cell strainer exhibited the highest efficiency for chicken embryo crypt harvesting.We found that transforming growth factor-b inhibitor(A8301)supplementation promoted enterocyte differentiation at the expense of the proliferation of intestinal stem cells(ISC).The mitogen-activated protein kinase p38 inhibitor(SB202190)promoted intestinal organoid formation and enterocyte differentiation but suppressed the differentiation of enteroendocrine cells,goblet cells and Paneth cells.However,the suppression of enteroendocrine cell and Paneth cell differentiation by SB202190 was alleviated at the presence of A8301.The glycogen synthase kinase 3 inhibitor(CHIR99021),valproic acid(VPA)alone and their combination promoted chicken intestinal organoid formation and enterocyte differentiation at the expense of the expression of Paneth cells and goblet cells.Chicken serum significantly improved organoid formation,especially in the presence of A8301,SB202190,CHIR99021,and VPA,but inhibited the differentiation of Paneth cells and enteroendocrine cells.Chicken serum at a concentration of 0.25%meets the requirement of chicken intestinal organoid development,and the beneficial effect of chicken serum on chicken intestinal organoid culture could not be replaced by fetal bovine serum and insulin-like growth factor-1.Moreover,commercial mouse organoid culture medium supplemented with A8301,SB202190,CHIR99021,VPA,and chicken serum promotes chicken organoid budding.Based on the chicken intestinal organoid model,we found that methionine deficiency mimicked by cycloleucine suppressed organoid formation and organoid size,and this effect was reinforced with increased cycloleucine concentrations.Methionine hydroxy analogue promoted regeneration of ISC but decreased cell differentiation compared with the results obtained with L-methionine.In conclusion,our results provide a potentially excellent guideline for chicken intestinal organoid culture and insights into methionine function in crypt development. | Youli Wang Qihang Hou Yuqin Wu Yanwei Xu Yan Liu Jing Chen Lingling Xu Yuming Guo Shuai Gao Jianmin Yuan | 2022 | Animal Nutrition2022，，1： | 4 |
| 6 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 7 | A multicenter retrospective cohort study of ketogenic diet therapy in 481 children with infantile spasms显示文摘Background:Ketogenic diet(KD)therapy is one of the main treatments for drug-resistant epilepsy.However,the KD therapy has been applied in only a small number of infantile spasm cases.In this large multicenter study,we investigated the efficacy of KD therapy in the treatment of infantile spasms.Methods:In this retrospective,multicenter cohort study,clinical data from main epilepsy centers were analyzed.Patients were classified into different groups according to age,type of drug and whether glucocorticoid was used before initiation of KD.Results:From October 2014 to March 2020,481 patients(308 males and 173 females)with infantile spasms were treated with the KD therapy.The age of the patients ranged from 2 months to 20 years,with a mean age of 1 year and 10 months.The number of anti-seizure medications(ASMs)used before KD initiation ranged 0-6,with a median of 3.In different time from initiation(1,3,6,and 12 months),the rates of seizure freedom after KD were 6.9,11.6;16.0 and 16.8%,respectively(x^(2)=27.1772,P<0.0001).There was a significant difference in the rate of seizure freedom between 3 months and 1 month(x^(2)=6.5498,P=0.0105)groups,and 6 months and 3 months(x^(2)=3.8478,P=0.0498)groups,but not between 12 months and 6 months(x^(2)=0.1212,P=0.7278)groups.The rates of effectiveness were 44.7;62.8,49.1 and 32.0%(x^(2)=93.2674,P<0.0001),respectively.The retention rates were 94.0,82.5,55.7 and 33.1%(x^(2)=483.7551,P<0.0001),correspondingly.The rate of effectiveness and the retention rate of KD were significantly different among the 1,3,6 and 12 months.KD treatment was the first choice in 25 patients(5.2%),55 patients(11.4%)started KD after the failure of the first ASM,158 patients(32.8%)started KD after the failure of the second ASM,157 patients(32.6%)started KD after the failure of the third drug,and 86 patients(17.9%)started KD after the failure of the fourth and more.The KD effect was not related to the number of ASMs used before KD startup(P>0.05).Two hundred and eighteen patients(45.3%)failed to respond to corticotropin or glucocorticoid before initiation.There was no significant difference in the effectiveness rate at different time points between the group of KD therapy after glucocorticoid failure and the group after non-hormone failure (x^(2)=0.8613,P=0.8348).The rate of adverse events of KD in 1,3,6,and 12 months after KD initiation were 22.3,21.7,16.8 and 6.9%,respectively.The adverse events mainly occurred during the first 3 months of KD,and the main adverse events were gastrointestinal disturbance and constipation.Conclusions:The efficacy of the KD treatment for infantile spasms was not affected by age,medication,and glucocorticoid use before initiation.KD is one of the effective treatments for infantile spasms. | Yuanzhen Ye Dan Sun Hua Li Jianmin Zhong Rong Luo Baomin Li Dengna Zhu Dan Li Shaoping Huang Yuwu Jiang Nong Xiao Yucai Chen Yuqin Zhang Mei Yu Xiaoyun Shen Li Gao Guo Zheng Congmin Zhao Baoqiang Yuan Jianxiang Liao Jiong Qin CAAE KD group | 2022 | Acta Epileptologica2022，5，1： | 2 |
| 8 | Animal exercise studies in cardiovascular research:Current knowledge and optimal design--A position paper of the Committee on Cardiac Rehabilitation,Chinese Medical Doctors’Association显示文摘Growing evidence has demonstrated exercise as an effective way to promote cardiovascular health and protect against cardiovascular diseases However,the underlying mechanisms of the beneficial effects of exercise have yet to be elucidated.Animal exercise studies are widely used to investigate the key mechanisms of exercise-induced cardiovascular protection.However,standardized procedures and well-established evaluation indicators for animal exercise models are needed to guide researchers in carrying out effective,high-quality animal studies using exercise to prevent and treat cardiovascular diseases.In our review,we present the commonly used animal exercise models in cardiovascular research and propose a set of standard procedures for exercise training,emphasizing the appropriate measurements and analysis in these chronic exercise models.We also provide recommendations for optimal design of animal exercise studies in cardiovascular research,including the choice of exercise models,control of exercise protocols,exercise at different stages of disease,and other considerations,such as age,sex,and genetic background.We hope that this position paper will promote basic research on exercise-induced cardiovascular protection and pave the way for successful translation of exercise studies from bench to bedside in the prevention and treatment of cardiovascular diseases. | Yihua Bei Lei Wang Rongjing Ding Lin Che Zhiqing Fan Wei Gao Qi Liang Shenghui Lin Suixin Liu Xiao Lu Yuqin Shen Guifu Wu Jian Yang Guolin Zhang Wei Zhao Lan Guo Junjie Xiao | 2021 | Journal of Sport and Health Science2021，10，6： | 2 |
| 9 | The roles of ADIPOQ genetic variations in cancer risk: evidence from published studies显示文摘 | Yeqiong Xu Bangshun He Yuqin Pan Ling Gu Zhenlin Nie Liping Chen Rui Li Tianyi Gao Shukui Wang | 2013 | Molecular Biology Reports2013，，2： | 1 |
| 10 | Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice显示文摘 | Yuqin Men Aizhen Zhang Liwen Zhang Yecheng Jin Zhishuo Wang Jing Zhao Xiaolin Yu Jian Zhang Jiangang Gao | 2016 | Frontiers of Medicine2016，10，4： | 1 |
| 11 | Further Understanding of the Food Safety Problem显示文摘Frequent occurrence of food quality and safety proves that it is not effective to solve the Problem only from mechanism and supervision mechanism. Instead,it may expand solution ideas from external environment inducing changes of social institutions. Edible agricultural products are raw materials of foods,so their quality and safety are decisive for food quality and safety. Combining with concept of quality and safety of edible agricultural products,from social economy,science,technology and culture,environment cognition,this paper made a further understanding of food quality and safety. It found that the quality and safety of domestic edible agricultural products are not completely resulted from human factor,and not completely quality and safety problem in practical sense. Design of problem solutions should consider such external factors as economic level and consumption concept,dual character of science and technology,cultural quality of the masses,and moral trait of the masses,and enhance matching of building of regulation tools with external environment. | Xingxing MEI Zhongchao FENG Pinghua HE Yawen GAO Yuqin DAI | 2015 | Asian Agricultural Research2015，7，7： | 1 |
| 12 | Preparation and blood compatibility of electrospun PLA/curcumin composite membranes显示文摘 | Yan Chen Jie Lin Yuqin Wan Yanna Fei Hongbo Wang Weidong Gao | 2012 | Fibers and Polymers2012，，10： | 1 |
| 13 | Comparative studies on thermal performance of water-in- glass evacuated tube solar water heaters with different collector tilt-angles 显示文摘 | Tang Runsheng Yang Yuqin Gao Wenfeng | 2011 | Solar Energy2011，85，7： | 1 |
| 14 | Observations of nonlinear oscillation phenomena in metallic solids显示文摘 | Gao Houxiu Yang Yuqin Yang Jingwu | 1998 | Chinese Science Bulletin1998，43，15： | 1 |
| 15 | Observation of non-linear oscillation on solid surface 显示文摘 | Gao Houxiu Yang Yuqin Yang Jingwu | 1998 | Chinese Science Bulletin1998，43，15： | 1 |
| 16 | TiO_(2)@NH_(2)-MIL-125(Ti) composite derived from a partial-etching strategy with enhanced carriers' transfer for the rapid photocatalytic Cr(Ⅵ) reduction显示文摘Metal-organic frameworks (MOFs)-based composites have been widely applied as photocatalysts because of their synergistic effect between the two individual component.Herein,TiO_(2)@NH_(2)-MIL-125(Ti) nanocomposites which possess unsaturated titanium–oxo clusters,mesoporous structure,and intimate interface were successfully constructed via an in-situ distilled water-etched route.The X-ray photoelectron spectroscopy (XPS) measurements indicated strong electronic interaction between TiO_(2)and NH_(2)-MIL-125(Ti),confirming the formation of TiO 2@NH_(2)-MIL-125(Ti) nanocomposite.Photoelectrochemical and thermodynamics measurements showed that TiO_(2)@NH_(2)-MIL-125(Ti)nanocomposites have improved charge separation efficient and decreased transfer resistance of the carriers within the heterojunction interfaces,which facilitates the photoexcited electrons transfer and reduction of the Cr(VI) species.Therefore,the optimal TiO_(2)@NH_(2)-MIL-125(Ti)nanocomposite demonstrated superior performance compared to NH_(2)-MIL-125(Ti) and NH_(2)-MIL-125(Ti) derived TiO_(2).Based on the free radical trapping experiment and electron paramagnetic resonance (EPR) measurements,a possible type-II scheme was proposed for the enhanced photocatalytic activity over the TiO_(2)@NH_(2)-MIL-125(Ti) nanocomposite. | Fang Xu Wanning Cao Jinzhou Li Songsong Zhi Zhiyong Gao Yuqin Jiang Wei Li Kai Jiang Dapeng Wu | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，4： | 0 |
| 17 | WTAP regulates postnatal development of brown adipose tissue by stabilizing METTL3 in mice显示文摘Brown adipocyte maturation during postnatal development is essential for brown adipose tissue(BAT)to protect animals against cold.Impaired maturation of brown adipocytes leads to cold intolerance.However,the molecular mechanisms that determine the maturation of brown adipocytes during postnatal development are not fully understood.Here,we identify Wilms’tumor 1-associating protein(WTAP)as an essential regulator in the postnatal development and maturation of BAT.BAT-specific knockout of Wtap(Wtap-BKO)severely impairs maturation of BAT in vivo by decreasing the expression of BAT-selective genes,leading to the whitening of interscapular BAT(iBAT).Single nucleus RNA-sequencing analysis shows the dynamic changes of cell heterogeneity in iBAT of Wtap-BKO mice.Adult mice with WTAP deficiency in BAT display hypothermic and succumb to acute cold challenge.Mechanistically,WTAP deficiency decreases m6A mRNA modification by reducing the protein stability of METTL3.BAT-specific overexpression of Mettl3 partially rescues the phenotypes observed in Wtap-BKO mice.These data demonstrate that WTAP/METTL3 plays an essential role in iBAT postnatal development and thermogenesis. | Yuqin Wang Xinzhi Li Cenxi Liu Liying Zhou Lei Shi Zhiguo Zhang Long Chen Ming Gao Lanyue Gao Yuanyuan Xu He Huang Jin Li Zheng Chen | 2022 | Life Metabolism2022，1，3： | 0 |
| 18 | Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms显示文摘Rheumatoid arthritis(RA)is a common chronic inflammatory disorder that usually affects joints.It was found that roburic acid(RBA),an ingredient from anti-RA herb Gentiana macrophylla Pall.,displayed strong anti-inflammatory activity.However,its medical application is limited by its hydrophobicity,lack of targeting capability and unclear functional mechanism.Here,we constructed a pH responsive dual-target drug delivery system hitchhiking RBA(RBA-NPs)that targeted both CD44 and folate receptors,and investigated its pharmacological mechanism.In rat RA model,the nanocarriers effectively delivered RBA to inflammatory sites and significantly enhanced the therapeutic outcomes compared with free RBA,as well as strongly reducing inflammatory cytokine levels and promoting tissue repair.Following analysis revealed that M1 macrophages in the joints were reprogrammed to M2 phenotype by RBA.Since the balance of pro-and anti-inflammatory macrophages play important roles in maintaining immune homeostasis and preventing excessive inflammation in RA,this reprogramming is likely responsible for the anti-RA effect.Furthermore,we revealed that RBA-NPs drove M1-to-M2 phenotypic switch by down-regulating the glycolysis level via blocking ERK/HIF-1α/GLUT1 pathway.Thus,our work not only developed a targeting delivery system that remarkably improved the anti-RA efficiency of RBA,but also identified a potential molecular target to reversely reprogram macrophages though energy metabolism regulation. | Na Jia Yunzhen Gao Min Li Yi Liang Yuwen Li Yunzhu Lin Shiqi Huang Qing Lin Xun Sun Qin He Yuqin Yao Ben Zhang Zhirong Zhang Ling Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，8： | 0 |
| 19 | Er-xian ameliorates myocardial ischemia-reperfusion injury in rats through RISK pathway involving estrogen receptors显示文摘Curculigo orchioides(CUR)and Epimedium(EPI)are traditional Chinese medicines with estrogen-like biological activity,called Xianmao and Xianlingpi(Er-xian)in Chinese.However,whether Er-xian exerts protective effects on myocardial ischemia-reperfusion injury(MIRI)is unknown.This study aimed to investigate the cardioprotective effects of Er-xian preconditioning against MIRI and the underlying mechanisms.CUR or EPI was administered intragastrically to aged female rats as a monotherapy or combination therapy.2 weeks later,a rat MIRI model was established.Myocardial infarction size,myocardial morphology,cTnT,cell apoptosis rate,intracellular calcium concentration,mitochondrial permeability transition pore(MPTP)opening and reperfusion injury salvage kinase(RISK)signaling pathway molecules were observed after the surgery.To evaluate the mechanisms of Er-xian,estrogen receptors antagonists ICI 182780 and G15 were used.In this study,Er-xian notably alleviated myocardial tissue damage,maintained mitochondrial morphology,reduced infarct size and cardiac markers,and increased sera levels of E2.Moreover,Er-xian inhibited calcium overload and mPTP opening,and decreased cardiomyocyte apoptosis.We found that the dual therapy of CUR and EPI elicited more noticeable results than CUR or EPI monotherapy.The significant protective effects of Er-xian on ischemia-reperfusion myocardium were attributed to the up-regulation of AKT,ERK1/2 and GSK-3βphosphorylation levels.The cardioprotective effects of Er-xian were significantly reduced after estrogen receptor blockade,especially GPER30.These results indicate that Er-xian attenuates MIRI through RISK signaling pathway and estrogen receptors are the critical mediators. | QIANG Mingmin HAO Jiping LIU Huihui YIN Jia ZHANG Hui YANG Jinxin MENG Hudie CHEN Yuqing GAO Yuqin | 2022 | Chinese Journal of Natural Medicines2022，20，12： | 0 |
| 20 | Tprn is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice显示文摘Tprn encodes the taperin protein,which is concentrated in the tapered region of hair cell stereocilia in the inner ear.In humans,TPRN mutations cause autosomal recessive nonsyndromic deafness(DFNB79)by an unknown mechanism.To determine the role of Tprn in hearing,we generated Tprn-null mice by clustered regularly interspaced short palindromic repeat/Cas9 genome-editing technology from a CBA/CaJ background.We observed significant hearing loss and progressive degeneration of stereocilia in the outer hair cells of Tprn-null mice starting from postnatal day 30.Transmission electron microscopy images of stereociliary bundles in the mutant mice showed some stereociliary rootlets with curved shafts.The central cores of the stereociliary rootlets possessed hollow structures with surrounding loose peripheral dense rings.Radixin,a protein expressed at stereocilia tapering,was abnormally dispersed along the stereocilia shafts in Tprn-null mice.The expression levels of radixin andβ-actin significantly decreased.We propose that Tprn is critical to the retention of the integrity of the stereociliary rootlet.Loss of Tprn in Tprn-null mice caused the disruption of the stereociliary rootlet,which resulted in damage to stereociliary bundles and hearing impairments.The generated Tprn-null mice are ideal models of human hereditary deafness DFNB79. | Yuqin Men Xiujuan Li Hailong Tu Aizhen Zhang Xiaolong Fu Zhishuo Wang Yecheng Jin Congzhe Hou Tingting Zhang Sen Zhang Yichen Zhou Boqin Li Jianfeng Li Xiaoyang Sun Haibo Wang Jiangang Gao | 2019 | Frontiers of Medicine2019，13，6： | 0 |
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